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Development of SCAR Marker for Detection of Sex Expression in Papaya (Carica papaya L.) from Several Genetic Backgrounds , Sobir; Sriani Sujiprihati; Evalina C. Pandia
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.875 KB) | DOI: 10.24831/jai.v36i3.1382

Abstract

Papaya plants are hermaphrodite, pistillate, or staminate. Sex inheritance in papaya is determined by a single gene locus with three alleles of M which is dominant for maleness, MH for hermaphrodites and m which is recessive for femaleness. Only fruits from hermaphrodite plants are marketed since they have the necessary commercial characteristics, i.e., they are pear-shaped and have thicker flesh and a smaller internal cavity. Increased papaya yield has been limited mainly by the ratio of female to hermaphrodite (1: 2) plants normally occurring in orchards. This ratio causes great losses to papaya producers. Identification of seedlings sex during nursery stage is of prime iportance. In order to obtain simple DNA markers to identify sex expression in papaya, five SCAR markers of 20-21 primers were utilized. Examination of these markers into 24 genotypes of papaya from 12 populations of different genetic background revealed that pair of primer PKBT-5 had successfully differentiated male and hermaphrodite plants from female plants. Hence, PKBT-5 pair of primer can be utilized as DNA marker for sex expression character identification in papayas.   Key words: Papaya, sex expression, SCAR marker
The Study of Genetic Diversity and Relationships on Carica sp. by Means of Random Amplified Polymorphic DNA (RAPD) Analysis Ahmad Satori; Sriani Sujiprihati; , Sobir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 30 No. 1 (2002): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (122.417 KB) | DOI: 10.24831/jai.v30i1.1425

Abstract

The genus of Carica comprises 21 species, among them three species have been domesticated and cultivated. They are Carica papaya, Carica candamarcencis and Carica monoica. In Indonesia, two species have been cultivated viz. Carica papaya and Carica candamarcencis. In this study, four accessions of Carica papaya species and one accession of Carica candamarcencis species were analyzed using RAPD technique. We successfully amplified a total I of 40 fragments from these DNA genome by using 6 random primers with GC bases content:::: 60% of each primer. The number of fragments of each primer ranged from 5 to 8 averaged 6.7 fragments per primer. Out of total fragments, 90% showed as polymorphic ranged from 5 to 8 on average 6 fragments per primer. A dendogram based on the UPGMA- link method using Nei and Li similarity and Principal Component Analysis (PCA) plot separated the accessions into two main groups, the Carica papaya species on the one side and the Carica candamarcencis species on the other side. The result demonstrated that RAPD analysis was able to reveal genetic difference between Carica papaya and Carica candamarcencis species, as well as genetic diversity in Carica papaya species.
SCAR (Sequence Characterized Amplified Region) Analysis for Pi-b and Pi-ta genes on 28 Genotypes of Rice , Sobir; , Santoso; Hajrial Aswidinnoor; Mukelar Amir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 1 (2005): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (435.578 KB) | DOI: 10.24831/jai.v33i1.1508

Abstract

Evaluation to blast disease (Pyricularia grisea) resistance was carried out by using two SCAR (Sequence Characterized Amplified Region) markers of Pi-b and Pi-ta blast resistance genes, and spray-inoculation method with 10 races of P. grisea on 28 paddy genotypes, that consisted six wild genotypes of rice. The results revealed that among 28 paddy genotypes, fifteen genotypes carrying both genes including Oryza rufipogon; six genotypes carrying Pi-b genes including O. alta, two genotypes carrying Pi-ta gene, and five genotypes did not possess both gene including O. glumaepatula, O. officinalis, O. latifolia, and O. malapuzhaensis. Based on infection intensity, the evaluated genotypes were vary in responses to different  ten races of P. grisea, indicated that the evaluated genotypes were  vary in carrying Pi genes. Analysis in detail indicated that existence of Pi-ta gene associated with lower infection intensity caused by P. grisea race 063 C.   Keywords:  SCAR markers, rice blast resistance, Pyricularia grisea
SCAR (Sequence Characterized Amplified Region) Analysis for Blast Resistant Evaluation on 12 Genotypes of Rice , Sobir; Harmi Andrianyta; Mukelar Amir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 31 No. 1 (2003): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.829 KB) | DOI: 10.24831/jai.v31i1.1526

Abstract

Resistance evaluation to blast disease (Pyricularia grisea) on 12 paddy genotypes was carried out in the green house by using spray inoculated method with race 033 and 041 of P. grisea , and SCAR (Sequence Characterized Amplified Region) marker by using Pib primer pairs. The results revealed that among 12 paddy genotypes were classified into six resistance groups. The first group comprised two genotypes (Jatiluhur and Asahan) having three resistance genes. The second group comprised two genotypes (Oryza malampuzhaensis and O. punctata) having two resistance genes against race 033 and 041. The third group had one resistance gene against race 033, comprised one genotype (Way Rarem). The fourth group comprised one genotype (Danau Tempe) having two resistance genes against 041 race and Pib. The fifth group comprised three genotypes (Kalimutu, Maninjau and Laut Tawar) having two resistance genes against race 033 and Pib. The sixth group comprised two genotypes (Kencana Bali and Cirata) having no resistance gene to blast race 033 and 041, and Pib.  These results indicated that Pib gene did not confer resistance to race 033 and 041 of Pyricularia grisea.  Resistance to race 033 and 041 might be controlled by different resistant gene.   Key words : SCAR, Blast resistant, Rice
Optimasi Pemanfaatan Tanaman Transgenik , Sobir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 29 No. 2 (2001): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (646.452 KB) | DOI: 10.24831/jai.v29i2.1549

Abstract

Human effort on improving agricultural crops has been expanding very rapidly, and recently by using gene transformation technology it was possible to introduce genes from biologically unrelated organisms into plant genomes. Gene transformation technology, known as transgenic technology, has been successfully introduced gene encoding insecticidal compound from bacteria Bacillus thuringiensis into several crops, for example. Introducing new technology is offering several advantages but also brings several disadvantages however since the technology isImportant for maintaining increase of crop production, it was necessary to optimize utilization of transgenic technology.   Key words: Gene transformation, Expression control, Antibiotic resistance
IDENTIFICATION OF CO-DOMINANT DNA MARKER TIGHTLY LINKED TO Tm-2 LOCUS IN TOMATO , Sobir
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 27 No. 3 (1999): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (975.622 KB) | DOI: 10.24831/jai.v27i3.1571

Abstract

Tm-2 adalah gen yang mengendalikan ketahanan terhadap tomato mosaic virus (ToMV) pada tomat (Lycopersicon esculentum) dan berasal dari Lycopersicon peruvianum. Pada penelitian ini digunakan dua galur tomat yang mempunyai latar belakang genetik yang sarna GCR26 dan GCR3 6, akan tetapi keduanya dapat dibedakan oleh adanya gen Tm-2 pada GCR36. Melalui analisis random amplified-polymorphic DNA (RAPD) terhadap dua galur tomat tersebut di atas diketahui adanya primer RAPD yang menghasilkan fragmen spesifik untuk GCR236. Selanjutnya fragmen tersebut sekuennya dianalisis, dan dari hasil analisis sekuen tersebut dibuat sepasang primer sequence characterized amplified region (SCAR) Yang merupakan marka DNA kodominan yang bisa membedakan baik genotipe homozigot gen Tm-2. Oleh karena fragmen dihasilkan terkait dengan Tm-2, maka primer SCAR tersebut merupakan sarana yang dapat digunakan untuk pemulian tanarnan tomat bagi pembentukan varietas tahan virus.
Karakter Fisik dan Kimia Buah Pepaya pada Stadia Kematangan Berbeda Ketty Suketi; Roedhy Poerwanto; Sriani Sujiprihati; , Sobir; Winarso Drajad Widodo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 1 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.04 KB) | DOI: 10.24831/jai.v38i1.1678

Abstract

The objective of the experiment was to investigate the physical and chemical characteristics of three stadia of maturity based on a range of peel color from green to yellow or based on percentage of the yellow area of fruit peel (stadium 1 = 25-49 % yellow, stadium 2 = 50-74 % yellow, and stadium 3 = above 75 % yellow) on six genotypes of papaya. Each genotype exhibited different days to maturity for each stadium. The fruits of stadium 1, 2 and 3 for IPB 1 were picked at 130, 135, and 140 days after anthesis (DAA); IPB 10A at 160, 165, and 170 DAA;  IPB 1 x PB 174 at 135, 140 and 145 DAA; while PB 174,  IPB 1 x IPB 10A and IPB 10A x PB 174 were picked at 140, 145 and 150 DAA, respectively. The results indicated that peel firmness was affected by maturity stage on female fruit of IPB 10A. Maturity stage affected chemical characteristics of papaya included total soluble solids (TSS) content (IPB 10A, female fruit of  PB 174, female fruit of IPB 1 x IPB 10A, and female fruit of IPB 1 x PB 174), vitamin C content (hermaphrodite fruit of 10 A, female fruit of IPB 1 x IPB 10A) and juice pH (hermaphrodite fruit of  IPB 1).  IPB 1 genotype can be harvested at all stadia of maturity stage. Hermaphrodite and female fruit of IPB 10 A, female fruit of  PB 174, female fruit of  IPB 1 x IPB 10A and female fruit of IPB 1 x  PB 174 genotype would be better harvested at stadium 3 of maturity stage.   Keywords: Carica papaya, papaya genotype, hermaphrodite fruit, female fruit, fruit quality, fruit maturity stage
Analisis Kedekatan Hubungan antar Genotipe Pepaya Berdasarkan Karakter Morfologi dan Buah Ketty Suketi; Roedhy Poerwanto; Sriani Sujiprihati; , Sobir; Winarso D. Widodo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 38 No. 2 (2010): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (255.631 KB) | DOI: 10.24831/jai.v38i2.1797

Abstract

<!-- /* Font Definitions */ @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri; mso-fareast-font-family:"Times New Roman"; mso-bidi-font-family:"Times New Roman"; mso-ansi-language:IN; mso-no-proof:yes;} @page Section1 {size:612.0pt 792.0pt; margin:72.0pt 90.0pt 72.0pt 90.0pt; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> A study was conducted to determine the variation and relationships among papaya genotypes based on morphological and fruit characteristics in order to produce high quality papaya fruits. Fruit characterization study is very useful for genotype improvement and genotype classifi cation of papaya. In this study the morphological characters of 36 genotypes were analyzed to determine their phenotypic variabilities. The relationships between genotypes based on all of the morphological and fruit characteristics were tested by subjecting the data to multivariate principal component analysis and to cluster analysis. Based on the dendrogram generated from vegetative and generative characters, the 36 genotypes could be grouped into 11 clusters on a threshold of 1.6 and formed 6 clusters on a threshold of 1.8. The dendrogram was able to explain the close relationship between IPB 5 x IPB 1 and IPB 5 x IPB 4, IPB 2 and IPB 7, IPB 1 and IPB 3 genotype. The scattered diagram of generative variable divided the papaya genotypes into three groups based on fruit sizes i.e small group (IPB 1, IPB 3, IPB 4, IPB 3 x IPB 4, IPB 1 x IPB 9), medium group (IPB 5, IPB 7, IPB 8, IPB 9) and big group (IPB 2, IPB 10). The IPB 1, IPB 3 and IPB 4 were different from IPB 2 in fruit shapes, petal length of male fl owers, infl orescence size and fruit length. The hybrid plants obtained from crossings with IPB 10 were different from the other genotypes in the colours of female-, hermaphrodite-, and male fl ower-lobes. Subsequently the scatter diagrams also revealed that several genotypes i.e. IPB 2 x IPB 6, IPB 1 x IPB 5, IPB 1 x IPB 9, IPB 5 x IPB 1 and IPB 5 x IPB 2 had superior characters ideotype similar to IPB 1, IPB 3 and IPB 8 genotypes. Keywords: Carica papaya, hermaphrodite, female, dendrogram, scatter diagram, ideotype
Pengaruh Batang Bawah dan Jenis Tunas pada Mikrografting Manggis (Garcinia mangostana) secara In Vitro Rd. Selvy Handayani; Roedhy Poerwanto; , Sobir; Agus Purwito; Tri Muji Ermayanti
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.152 KB) | DOI: 10.24831/jai.v41i1.7076

Abstract

The aim of this study was to investigate the effect of rootstock and shoot types on in vitro mangosteen micrografting.The experiment was arranged in completely randomized design (CRD) with two factors. The first factor was the rootstocktype, i.e. rooted planlet from the germination of quartered seed, and rooted planlet from the germination of undivided seeds.The second factor was the developmental phase of scion, i.e. dormant buds, and flush (had new leaf more than 2-4 mm). Theresults showed that rootstock derived from the germination of undivided seed had a higher success rate than other treatmentson all variables, except for number of new leaves. The use of flush as scion was better than dormant buds; flush resulted in ahigher percentage of successful micrograft and longer shoots. In vitro micrografting had a better growth rate than grafting at the same age. The results of anatomical observation conducted at four months after micrografting demonstrated that there was a good graft union, indicated by excellent fusion between rootstock and scion xylem tissues.Keywords: flush, in vitro, micrografting, rootstock, scion
Analisis Genetik Sifat Ketahanan Melon (Cucumis melo L.) terhadap Virus Kuning Entit Hermawan; Sobir ,; Darda Efendi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 2 (2014): Jurnal agronomi indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.511 KB) | DOI: 10.24831/jai.v42i2.8823

Abstract

ABSTRACTResistance to yellow virus (YV) is an important breeding trait in melon. However information regarding genetic inheritance pattern of the character are limited. This study aimed to provide information on genetic control for resistance to YV in melon caused by Tomato Yellow Leaf Curl Virus (TYLCV). Twenty genotypes from three major melon groups (dudaim, cantaloupe, and inodorous) were evaluated using controlled inoculation method. The results revealed that one line, MEV1 from the dudaim group, showed high resistance to YV; while other lines belong to cantaloupe and indororus indicated as highly susceptible lines. Screening of the F1 from crossing between resistant and susceptible parents resulted in resistant F1 after inoculation and planted in endemic location. Subsequently, evaluation on F2 population revealed a non-normal distribution for disease severity score, indicating that resistance to YV in melon was controlled by major genes. Chi-square (χ2) test resulted in 13:3 ratio and indicated that the resistance to YV was controlled by 2 genes pair with dominant and epistasis recessive actions.Keywords: dominant and epstasis recessive action, major gene, Bemicia tabaci, Begomovirus
Co-Authors , Kisman , Santoso Achmad Baihaki Adinda Wuriandani Agung Wahyu Susilo Agus Purwito Ahmad Dadang Ahmad Satori Ahmad Yunus Aji Hermawan ALEX HARTANA Alex Hartana Alex Hartana ALFRED P. MANAMBANGTUA Alifiya Herwitarahman Anas Dinurrohman Susila Andi Azhari Andika Septiana Suryaningsih Aris Purwanto Arisanti, Tiffani Nindya Arya Widura Rtionga Astri W Wulandari Astri Windia Wulandari Wulandari Awang Maharijaya Baiq Arriyadul Badi'ah Bonjok Istiaji Budi Santosa C Hanny Wijaya Chesaria, Nanda DADANG, AHMAD Deden Derajat Matra Delvi Maretta Desta Wirnas Didy Sopandie Diny Dinarti Diyah Martanti, Diyah DONATA S. PANDIN Dyra Haryanti Edi Santosa Edi Santosa Edi Santosa Efendi, D. Efendi, Darda Eka Fatmawati Tihurua Eko Priyantono Ellina Mansyah Entit Hermawan Eny Widajati Erni Suminar Euis Nining Evalina C. Pandia Freestina Andarwening Fusao Motoyoshi Gunawan Gunawan Gunawan Gunawan Hafizah, Rumaisha Afifatul HAJRIAL ASWIDINNOOR Hanifah Nuryani Lioe Hanik Rohmah Robi'ah Harmi Andrianyta Heri Harti Herry Suhardiyanto Hidayat , Sri Hendrastuti I Wayan Budiastra Ika Roostika Imam Widodo Iman Rusmana Imas Sukaesih Sitanggang Indah Kurniasari Irvan Faizal Is Helianti Islah Hayati J. K. J. Laisina Joko Prasetiyono Juliati S. Darsa Juliet M.E. Mamahit Ketty Suketi Khairiyah, Hayat Khamidi, Thamrin Kisman Kisman Kristianto Nugroho Kurniawan Rudi Trijatmiko Kuswandi Kuswandi La Ode Safuan La Ode Safuan Laksono Trisnantoro Lilik Pujantoro Lina Herlina Lina Herlina Lina Herlina Lizawati Lizawati M A Chozin M. Syamsul Maarif Machfud Machfud Mahat Magandhi MANAMBANGTUA, ALFRED P. Mardiana - MARIA BINTANG Marlin Marlina Mustafa, Marlina MATHIUS, NURITA TOURAN Matra, Deden Derajat Meddy Rachmadi Memen Surahman Mien A. Rifai Miftahudin . Muchlis . Muhamad Syukur Muhammad Arif Nasution Muhammad Syukur Mukelar Amir Munarti Nazli, Rizal Sjarief Sjaiful Nazly Aswani Nettyani, Naipospos Ni Made Armini Wiendi Nobuo Sugiyama Noorrohmah, Siti Nurita Toruan-Mathius Nurul Khumaida Nurwahyuningsih Nurwahyuningsih OTIH ROSTIANA PANDIN, DONATA S. Parjanto Parjanto Poetri Agustine Aryawati PRASETIYONO, JOKO Prawestri, Apriliana Dyah Priyanti Priyanti Priyanti Priyanti Priyantono, Eko PURNAMA HIDAYAT Purwono Purwono Putra, Fiqhri Mulianda Rahmadara, Gemilang Rahmi Yunianti Ramadhani Dwi Santoso Ramadhani Dwi Santoso Rd. Selvy Handayani Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Ridwan Setiamihardja Risa Aryantri Ritonga, Arya Widura Roberdi , Roedhy Poerwanto Rosmaina Rosmaina S H Hidayat S Noorrohmah Santosa, Budi Santosa, Budi Sarsidi S astrosumarjo Sayekti, Tri Wahono Dyah Ayu Sekar Wulan Prasetyaningtyas Siti Hafsah Siti Hartati Yusida Saragih Soaloon Sinaga SOLIN, NIDA W.N.M. SRI HENDRASTUTI HIDAYAT Sriani Sujiprihati Sriani Sujiprihati Sriani Sujiprihati Sudarmono Sudirman Yahya Sujiprihati, Sriani Sulistijorini Sunyoto Sunyoto Suparjo Suprayanti Martia Dewi Suprayanti Martia Dewi, Suprayanti Martia Surjono Hadi Sutjahjo Suryo Wiyono Susan Helmi SYAFRIDA MANUWOTO Syarifah Iis Aisyah Syukur, M Tamami, Djoko TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN TATIK CHIKMAWATI Tengku Laila Kamaliah Teuku Tajuddin Tias Arlianti Tony Liwang Tri Budiyanti Tri Handayani Tri Muji Ermayanti Tri Muji Ermayanti Trijatmiko, Kurniawan Rudi Trikoesoemaningtyas U Undang Utami Prawati Wida W. Khumaero Widodo Widodo Willy Bayuardi Suwarno Winarso D. Widodo Winda Nawfetrias Witjaksono Wulan Septiningtyas Kurniajati Yoko Mine Yudiwanti Wahyu E. Kusumo Yukarie Ayu Wulandari Yukarie Ayu Wulandari Yusnita Sari Zainal Alim Mas’ud Zumaidar Zumaidar