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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Jurnal Primatologi Indonesia Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Hemera Zoa Media Veteriner Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Ilmu Ternak Veteriner Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) ARSHI Veterinary Letters JURNAL BIOLOGI PAPUA Jurnal Kedokteran Hewan Jurnal Veteriner dan Biomedis
Srihadi Agungpriyono
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary Other

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Journal : Jurnal Veteriner

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INJEKSI SPERMATOZOA DOMBA HASIL PENGERINGBEKUAN KE DALAM SEL TELUR MENGGUNAKAN TEKNIK INTRACYTOPLASMIC SPERM INJECTION (ICSI) INJECTION OF FREEZE-DRIED RAM SPERMATOZOA INTO OOCYTES USING INTRACYTOPLASMIC SPERM INJECTION, ICSI Takdir Saili; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Pada penelitian ini dikaji kemampuan spermatozoa domba hasil pengeringbekuan untuk melakukan dekondensasi dan membentuk pronukleus setelah diinjeksikan ke dalam oosit dengan menggunakan teknik intracytoplasmic sperm injection (ICSI). Metode pewarnaan aceto lacmoid digunakan untuk mengevaluasi kejadian dekondensasi dan pembentukan pronukleus pada oosit setelah ICSI. Hasil penelitian menunjukkan bahwa spermatozoa hasil pengeringbekuan dapat melakukan dekondensasi (2%) dan mendukung pembentukan 1PN (34%) tetapi belum mampu mendukung pembentukan 2PN setelah diinjeksikan ke dalam oosit. Sedangkan injeksi dengan menggunakan spermatozoa segar mampu mendukung pembentukan 2PN (30%) dan 1PN (40%). Sebagai kesimpulan dapat dikemukakan bahwa spermatozoa hasil pengeringbekuan mampu melakukan dekondensasi dan mendukung pembentukan 1PN setelah ICSI
Penentuan Siklus Estrus pada Kancil (Tragulus javanicus) Berdasarkan Perubahan Sitologi Vagina Najamudin -; Rusdin -; Sriyanto -; Amrozi -; Srihadi Agungpriyono; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to determine the estrus cycle and the length of estrus in Tragulus javanicuson the basis of its vaginal cytology. Vaginal swabs were collected daily at 7 am for two months. Smears ofthe swab were then prepared on glass slide and they were stained with Giemsa. Vaginal epithelial cells;parabasal, intermediet and superficial cells were counted and their percentages during proestrus, estrusand diestrus were determined. Diestrus was characterized by the absent of superficial cells in the vaginalepithel. Proestrus was characterized by the progressive increase in percentage of intermediet/superficialcells in vaginal epithel, whereas estrus was characterized by the presence of superficial/cornification cellsin most vaginal epithel. On the basis of its vaginal cytology, it was determined that the estrous period offemale Tragulus javanicus was 3 days (48-60 h) and the length was 11 days (10-14 day). The change in thecytology of vaginal epithelial cells appeared to a good marker to determine the estrus cycle phase of theTragulus javanicus
PERUBAHAN VIABILITAS DAN STRUKTUR SUBSELULER SPERMATOZOA DOMBA SETELAH PENGERINGBEKUAN Takdir Saili; I Ketut Mudite Adnyana; Ronny Rachman Noor; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 10 No 4 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Several methods i.e. cooling, freezing, and freeze-drying have been widely used to preserve spermatozoa with various degree of success. Freeze-drying appears to provide a method to preserve spermatozoa in a dry state without requiring liquid nitrogen for storing frozen spermatozoa. Freeze-drying procedures can have a detrimental effect on plasma membrane and acrosomal cap of the spermatozoa. In this experiment study, the viability and subcellular changes of freeze-dried ram spermatozoa were evaluated using staining method and scanning electron microscopy. The results revealed that all freeze-dried spermatozoa were dead following evaluation using eosin staining and Hoechst-propidium iodide staining methods. Morover, plasma membrane and acrosomal cap of freeze-dried ram spermatozoa was disrupted observed using scanning electron microscope.
STUDY ON SPERM AGGLUTINATION WITH CHARACTERIZATION OF PLASMACOLLECTED FROM EPIDIDYMIS AND EJACULATE IN RAM Muhammad Haviz; Arief Boediono; M Agus Setiadi; Srihadi Agungpriyono; Mokhamad Fahrudin
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was designed to optimalize the use of epididymal or ejaculate sperm and plasma for in vitro fertilization, that sperm agglutination was found at preparation. The rate of sperm agglutination was calculated the head-to-head sperm agglutination that were incubated in Krebs Ringer-(N-(2hydroxyethyl)piperazine-N’-(2-ethenesulfonic acid) or KR-HEPES medium in 38.50C with 5% CO2 at 1, 3, 5 and 7 hours culture in vitro. The rate of head-to-head sperm agglutination were decreased with time treatments. The cauda of sperm agglutination was lower than that caput, corpus epididymal and ejaculate sperm with statistically significant (P<0.01). These result reflected that distribution of anti-agglutinin might be higher in cauda epididymal than that other areas. Number of protein were characterize with SDS-PAGE as follow 11 bands in caput epididymal, 9 bands in corpus epididymal, 2 bands in cauda epididymal and 4 bands in seminal plasma. The higher distribution of protein was found at range 25-40 kDa in epididymal plasma of ram. However, further investigation should be conducted to determine presumptive anti-agglutinin by advance method.
SEBARAN ANTIAGLUTININ SPERMATOZOA DALAM PLASMA YANG DIKOLEKSI DARI EPIDIDIMIS DAN EJAKULAT DOMBA THE DISTRIBUTION OF SPERM ANTIAGGLUTINI IN PLASMA COLLECTED FROM EPIDIDYMIS AND EJACULATE OF RAM Muhamad Haviz; Srihadi Agungpriyono; Arief Boediono; Mokhamad Fahrudin; M Agus Setiadi
Jurnal Veteriner Vol 8 No 1 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Penelitian untuk mengetahui tingkat aglutinasi antarkepala spermatozoa dan sebaran antiaglutinin dalam plasma asal epididimi dan ejakulat telah dilakukan untuk mengoptimalkan pemanfaatannya dalam fertilisasi in vitro. Tingkat aglutinasi dan aktivitas antiaglutinin plasma epididimis dan ejakulat domba dihitung dengan cara menghitung jumlah aglutinasi antarkepala spermatozoa setelah diinkubasikan selama 1, 3, 5, dan 7 jam in vitro dalam media Krebs Ringer- HEPES.
Tingkat Proliferasi Primordial Germ Cells secara In Vitro dalam Medium Kultur dengan Penambahan Leukemia Inhibitory Factor (IN VITRO PROLIFERATION RATE OF MICE PRIMORDIAL GERM CELLS IN THE CULTURE MEDIUM WITH ADDITION OF LEUKEMIA INHIBITORY FACTOR) Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Srihadi Agungpriyono; Mokhamad Fahrudin
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.304 KB) | DOI: 10.19087/jveteriner.2019.20.4.526

Abstract

Primordial germ cells (PGCs) are precursors for gamete cells. The totipotency of PGCs allows them to be used as a model for studying cancer and infertility. The study aimed to examine the characteristics of the mice fetus as a source of PGCs, proliferation rate of PGC and the role of LIF in vitro culture of PGCs. This study used genital ridges from 26 fetuses at 13.5 days post-coital (dpc) to isolate the PGCs. Genital ridges dissociation using 0.1% of trypsin and in vitro culture was carried out using the Dulbecco Modified Eagle Medium (DMEM) and incubated at 37 0C and 5% CO2 atmosphere. The fetus was measured and weighed to determine the normal development of the fetus and continued with the identification of the genital ridges after laparotomy performed under a stereomicroscope. Proliferation rate was measured by calculating Population Doubling Time (PDT), and cell viability was observed after in vitro culture for six days. The effect of adding 1000 IU/ml of leukemia inhibitory factor (LIF) was evaluated from two types of treatment in the medium, 1) DMEM added with 15% of fetal calf serum (FCS) (DMEM + S15%) and 2), DMEM was supplemented with 15% of FCS and 1000 IU/ml LIF (DMEM + S15% + LIF1000 IU/ml). Immunohistochemistry staining was carried out on the third-, sixth- and ninth-day of culture to detect the expression of Oct-4 in the PGCs, then cells were counted. The results showed that the fetus as a source of PGCs had normal development. The fetal sizes were 11 mm, and male and female genital ridges could be distinguished by morphology at the age of 13.5 dpc. The proliferation of PGCs was relatively slow with a 1.3 day PDT value with the viability of around 85%. Culture of PGCs with DMEM + S15% treatment showed the percentage of PGCs that expressing Oct-4 decreased from the third day of culture to the ninth day of culture. The culture of PGCs in DMEM + S15% + LIF 1000 IU / ml treatment showed that the percentage of PGCs that expressed Oct 4 increased on the sixth day of culture and decreased on the ninth day of culture. It can be concluded that the addition of LIF can maintain the number of PGCs until the sixth day of culture. LIF is thought to play a role in the regulation of proliferation of PGCs through receptors of LIF (RLIF) and glicoprotein (gp) 130 receptors.
Histologi dan Histomorfometri Testis dan Epididimis Muncak (Muntiacus muntjak muntjak) pada Periode Ranggah Keras (HISTOLOGY AND HISTOMORPHOMETRY OF THE TESTIS AND EPIDIDYMIS OF MUNTJAC (MUNTIACUS MUNTJAK MUNTJAK) DURING HARD ANTLER PERIOD) Sri Wahyuni; Srihadi Agungpriyono; Muhammad Agil; Tuty Laswardi Yusuf
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to describe the histology and histomorphometry of testis and epididymisof muntjac (Muntiacus muntjak muntjak) during hard antler period. The tissues of the testis and epididymisof an adult male muntjac were processed for histological examination and stained with haematoxylineosine(HE). The parenchyma of muntjac’s testis during hard antler period showed tubuli seminiferi waslined with germinal epithelium: spermatogonia, spermatocyte, spermatid that differentiated intospermatozoa. Sertoli cells were found among the germinal cells. In addition, Leydig cells were foundaround the blood vessel of interstitial tissue along with macrophages. Diameter of the seminiferous tubuleand epithelial thickness were 176,60±7,06 ?m and 50,27±3,62 ?m respectively. The epididymal duct wassubdivided into three segments: caput, corpus and cauda. They were lined predominantly withpseudostratified columnar epithelium which was varied in its thickness. The largest diameter of epididymalduct was found in cauda region (324,26±25,79 ?m), while caput epididymidis had the thickest of epithelialcell (62,21±4,21 ?m) and tended to ce thinner in corpus (49,53±3,01 ?m) and cauda epididymidis(16,30±2,27?m). The density of spermatozoa was observed the most in the lumen of cauda region comparedto caput and corpus epididymidis. In conclusion, the structure of histology and histomorphometry of theseminiferous tubule of testis and epididymal duct of muntjac were similar with small ruminants andother Cervidae during hard antler period.
Anatomi Makroskopis dan Analisis Dinamika Profil Darah pada Proses Penyembuhan Luka pada Kulit Landak Jawa (Hystrix javanica) (MACROSCOPIC ANATOMY AND HEMATOLOGICAL CHANGES DURING WOUND HEALING PROCESS IN THE SKIN OF THE JAVA PORCUPINE (Hystrix javanica)) Srihadi Agungpriyono; Andhika Yudha Prawira; Huda Salahudin Darusman; Wartika Rosa Farida; Savitri Novelina
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In Sunda Porcupine, wound healing occurred in a relative quick period, minimum scar formation, and without infection even though the wound is large and severe. The physiology which involved blood function as the systemic component is important in wound healing. This study aimed to acknowledged the macroscopic anatomy of the clinical signs, the role of blood component, and blood biochemistry in wound healing in Sunda porcupine (Hystrix javanica). The study used 6 adults of Sunda porcupine (3 males and 3 females). Stadium 3 wounds (full-thickness wound) were applied in thoracodorsal and lumbosacral region, approximately 10-11 cm2. Clinical signs observations were performed in day 0, 3, 7, 10, 14, 30, and 40, while blood collection were conducted in day 0, 7, 14, 21, 30, and 40 post wounding. The results of this study showed that wound closure occurred in 30-40 days post wounding with thoracodorsal wound closed faster than that in lumbosacral. Blood profile alteration during wound healing showed that wound healing occurred well. The increasing profile of erythrocytes and platelets during wound healing showed that these two components might become important of internal factor in wound closure, whereas the alteration of leucocytes showed no prolonged inflammation in wound healing in Sunda porcupine. The alteration of liver enzyme and renal function showed a fluctuation however the wound healing occurred well, whereas the decreased blood glucose level indicated no prolonged wound healing to chronic stage. These results give the information of clinical view and internal factors which contribute to the wound healing in the skin of Sunda porcupine.
Deteksi Umur Pubertas Muncak (Muntiacus muntjak muntjak) Betina Berdasarkan Analisis Metabolit Estrogen dan Progesteron pada Feses (THE AGE OF PUBERTY DETECTION IN FEMALE BARKING DEER (MUNTIACUS MUNTJAC MUNTJAC) BASED ON FAECAL ESTROGEN AND PROGESTERONE A Asri Pudjirahaju; Iman Supriatna; Srihadi Agungpriyono; Muhammad Agil
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Knowledge and information about the age of puberty in muntjac (Muntiacus muntjac muntjac) isindispensable to the interests of females breeding in conservation efforts. The aims of this study were todetermine the age of puberty and age at first mated females muntjac kept in captivity through the analysisof estrogen and progesterone metabolites in feces. This study used 155 fecal samples that were collectedfrom three female muntjacs. Sample collection was began when muntjac aged three months, four monthsand six months. Total of 10-20 g fecal samples were collected every 2-4 days. Analysis of steroid hormonemetabolites was performed by using enzyme immunoassay (EIA) method with specific antibodies.Determination of the age of puberty was based on the appearance of the first time estrus and ovulation,which was indicated by the appearance of the highest estrogens secretion, on hormone metabolites profile.Hormone metabolites data then were tabulated in the average and standard deviations were presentedwith graphs and analyzed descriptively. The results showed that the age of puberty detection based onanalysis of the estrogens and progesterone metabolite in the feces can be applied in muntjac. Muntjacfemales kept in captivity flats reached puberty at age 5±1 month or 4-6 months range. It is recommendedthe first mated in the muntjac is at least after the female experienced two period of oestrous or has reachedat age of six months.
Aktivasi Oosit Menggunakan Strontium Klorida setelah Injeksi dengan Spermatozoa Domba Hasil Pengeringbekuan (OOCYTE ACTIVATION USING STRONTIUM CHLORIDE FOLLOWING INJECTION OF FREEZE-DRIED RAM SPERMATOZOA) Takdir Saili; Ita Djuwita; Mohamad Agus Setiadi; Srihadi Agungpriyono; Arief Boediono
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

One of the factors that inhibit the formation of male pronuclei following injection of freeze-dried ramspermatozoa was the absence of artificial activation during oocyte incubation after the injection. Therefore,in this experiment the ability of strontium chloride (SrCl2) to improve oocyte activation followingintracytoplasmic sperm injection (ICSI) was evaluated. Aceto lacmoid staining was used to assessdecondensation and pronucleus formation following ICSI. Results of this experiment revealed that freezedriedspermatozoa had the ability to decondense and to form 1PN following injection into oocytes evenwithout artificial activation, but failed to form 2PN. However, 40% of 2PN oocytes were obtained when theinjected oocytes was first incubated for 20 minutes in medium containing 50 mM strontium chloride thensubsequently incubated for 10 hours in medium without strontium. On the contrary, the 2PN oocytes werenot observed either in injected oocyte neither without artificial activation nor in non-injected oocytes withartificial activation. In conclusion, freeze-dried ram spermatozoa were able to decondense and to support2PN formation following ICSI and artificial activation using strontium.
Co-Authors A.S. Satyaningtijas Adi Winarto Adrien Jems Akiles Unitly Adrien Jems Akiles Unitly, Adrien Afiff , Usamah Alastair A Macdonald Alastair A Macdonald, Alastair A Amaq Fadholly Amrozi Anak Agung Istri Sri Wiadnyani Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Andhika Yudha Prawira Angelina N. Tethool Anisa Rahma Anisa Rahma, Anisa Arief Boediono Aries Boediono Aryani Sismin Sastyaningtijas Asri Pudjirahaju Auzi Asfarian Bahasa Indonesia Bahasa Indonesia, Bahasa Indonesia Cahyadi, Danang C. Cahyadi, Danang D. Chairun Nisa Chairun Nisa Chairun Nisa Chairun Nisa’ Chairun Nisa’ Cutnya’ Shaliran Nazlie (Alm) Damiana Rita Ekastuti Danang Dwi Cahyadi Dedi Rahmat Setiadi Deni Noviana Denny W. Lukman Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Desrayni Hanadhita Dondin Sajuthi Dwi Endrawati Dwi Endrawati Dwi Kesuma Sari Dwinna Aliza Dwinna Aliza EFFENDIE, ICHSAN Eragilang Muhammad Hastapatria Erdiansyah Rahmi Erni Sulistiawati Etih Sudarnika Etty Riani Evalina E Evalina E, Evalina Fadli A. Gani Gani, Fadli A. Gholib Gholib Gholib Gholib, Gholib GONO SEMIADI Gunanti . Hafizuddin Hafizuddin Halim Bakti Harjo Hamny H, Hamny Hamny Hamny Hamny Sofyan Hanadhita, Desrayni Hany Savitry Hellen Kurniati, Hellen Helny Rosita Supriadi Helny Rosita Supriadi Helny Rosita Supriadi Heni Pujiastuti HERA MAHESHWARI Heru Setijanto Heru Setijanto Herwin Pisestyani Hidayah, Dinda Nur Huda Salahudin Darusman Huda Shalahudin Darusman I Ketut Mudite Adnyana I. Ketut Mudite Adnyane, I. Ketut Mudite Idawati Nasution Idawati Nasution Idawati Nasution Iman Supriatna Ismahyuningsih, Revita Istiqmal, Kahfi Aulia Ita Djuwita ITA DJUWITA Ita Djuwita Junzo Yamada Ketut Adnyane Mudite Ketut Mudite Adnyane, Ketut Mudite Kusdiantoro Mohamad Lidya Elizabeth M. Manik M Agus Setiadi M. Haviz M. Zairin Junior Maheshwari2, Hera Maker, Ursula Paulawati Masaji Washio Md Zuki Abu Bakar Mirnawati Sudarwanto Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad MOZES R. TOELIHERE Mozes Toelihere MUHAMMAD AGIL Muhammad Jalaluddin Muhammad Jalaluddin, Muhammad Muhammad Risman Wahid Mulyadi Adam Mulyadi Adam, Mulyadi MULYOTO PANGESTU Muslim Akmal Nabuo Kitamura Narindria, Yasmin Nadhiva Nastiti Kusumorini Nastiti Kusumorini NASTITI KUSUMORINI Nazlie (Alm), Cutnya’ Shaliran Ni Luh Putu Rischa Phadmacanty Ni Wayan Kurniani Karja Nisa', Chairun Nisa’, Chairun Nisa’, Chairun Noordin Mohamed Mustapha Nuraini , Henny Nurhidayat Nurhidayat Nurhidayat Nurhidayat Nurhidayat, Nurhidayat - Odilia Rovara Prawira, Andhika Yudha Putri Syifa Camilla R. Iis Arifiantini Rahma Anisa Razali . RIDWAN AFFANDI Riki Siswandi Ronny Rachman Noor Rusdin - Savitri Novelina Simangunsong, Yanri R.N. Solly Aryza Sri Nurdiati Sri Wahyuni Sri Wahyuni Sri Wahyuni Sriyanto - Suparmin, Yuliani Supratikno Supratikno . Supratikno, Supratikno Syahruddin Said Syamsiar, Syamsiar TAKDIR SAILI Takdir Syahruddin Said Tongku Nizwan Siregar Trilaksono, Wahyu Tutik Wresdiyati Tuty L. Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf Tuty Laswardi Yusuf TUTY LASWARDI YUSUF Ursula Paulawati Maker Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahyuni, Sri Wartika Rosa Farida Wartika Rosa Farida Yoshio Yamamoto Yuliani Suparmin YUSUF, TUTI L