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All Journal HAYATI Journal of Biosciences VALENSI Bionatura Teknotan: Jurnal Industri Teknologi Pertanian Jurnal Natur Indonesia Microbiology Indonesia Majalah Kedokteran Bandung Indonesian Journal of Pharmaceutical Science and Technology LEMMA: Letters Of Mathematics Education Indonesian Journal of Biotechnology Jurnal Elemen Edumatsains QALAMUNA: Jurnal Pendidikan, Sosial, dan Agama Health Notions Jurnal Biodjati AKSIOLOGIYA : Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN JASMANI DAN OLAHRAGA Chimica et Natura Acta Jurnal Moral Kemasyarakatan JURNAL SAINS INDONESIA IndoMath: Indonesia Mathematics Education Jurnal Kimia Terapan Indonesia Jurnal Pendidikan Kimia (JPKim) Indonesian Journal of Chemistry IJCER (International Journal of Chemistry Education Research) Jurnal PAJAR (Pendidikan dan Pengajaran) Gelanggang Olahraga: Jurnal Pendidikan Jasmani dan Olahraga (JPJO) JOURNAL SPORT AREA FLUIDA Molekul: Jurnal Ilmiah Kimia Jurnal Pedagogik Olahraga Journal of Authentic Research on Mathematics Eduacation (JARME) Jurnal PORKES Physical Activity Journal (PAJU) JUMORA: Jurnal Moderasi Olahraga JES-MAT (Jurnal Edukasi dan Sains Matematika) Jurnal Ilmiah Berkala Sains dan Terapan Kimia Pharmaceutical Sciences and Research (PSR) Rangkiang Mathematics Journal International Journal of Educational Research Excellence (IJERE) Jurnal Sains dan Teknologi Farmasi Indonesia Euclid al Kimiya : Jurnal Ilmu Kimia dan Terapan ACTIVE: Journal of Physical Education, Sport, Health and Recreation Journal of Physical Education Health and Sport
Toto Subroto
Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Immunoinformatics Study on Early 4 Protein of Human Papillomavirus Type 16 for Cervical Cancer Vaccine Peptide Candidate Yani Suryani; Opik Taupiqurrohman; Muhammad Yusuf; Toto Subroto; Sukma Nuswantara
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.5414

Abstract

 The aims of this study were to carry out testing of the early 4 protein of type 16 HPV through immunoinformatics meth-ods in an effort to get the peptide vaccine candidate for cervical cancer. The software used are IEDB-AR, CABSdock and Accelrys Discovery Study 4.5. Based on the analysis that sequence of ami-no acid lysine, leucine, leucine, glycine, serine, threonine, tryp-tophan, proline and threonine (KLLGSTWPT) and the sequence of amino acid tyrosine, tyrosine, valine, leucine, histidine, leucine, cysteine, leucine, alanine, alanine, threonine, lysine, tyrosine, pro-line and leucine (YYVLHLCLAATKYPL) are peptide vaccine can-didate for cervical cancer from the early 4 protein of HPV type 16 
Generation of a bivalent dengue virus-like particle targeting DENV-1 and DENV-2 in Pichia pastoris Kusumawardani, Shinta; Yusuf, Muhammad; Yosua, Yosua; Syafrudin, Elza Nurrachmanita; Baroroh, Umi; Nurainy, Neni; Subroto, Toto
Indonesian Journal of Biotechnology Vol 31, No 2 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.114770

Abstract

Dengue remains a major global health concern, with current licensed vaccines offering variable protection across the four virus serotypes and posing safety considerations related to antibody-dependent enhancement (ADE). To contribute toward improved vaccine design, we developed a bivalent virus-like particle (VLP) targeting DENV-1 and DENV-2, the two most prevalent serotypes in Indonesia. Consensus envelope (CE) protein sequences from Indonesian DENV-1 and DENV-2 strains were codon-optimized and co-expressed in Pichia pastoris GS115 using the AOX1 promoter. The expressed proteins were solubilized from membrane fractions under denaturing conditions, purified via Ni2+-affinity chromatography, and subjected to urea gradient dialysis for VLP assembly. Transmission electron microscopy confirmed the formation of spherical VLPs. Western blot analysis confirmed expression and purification via anti-His detection, and supported antigenic integrity of the envelope proteins as recognized by anti-dengue E antibodies. The study demonstrates the feasibility of using P. pastoris for chimeric dengue VLP production based on regionally relevant strains. The findings support the development of serotype-focused VLP platforms as modular components in the development of future tetravalent dengue vaccines.
The Relationship Between Basic Locomotor Movement Ability and Learning Outcomes in Floor Gymnastics Aceng Hilman; Toto Subroto; Marisa Novianti Fajrah Ilsya
Journal of Physical Education Health and Sport Vol. 13 No. 1 (2026)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpehs.v13i1.42334

Abstract

Basic locomotor movement skills are part of fundamental movement skills (FMS), which develop in childhood and form an important foundation for mastering more complex specific motor skills. Basic movement skills that do not develop optimally will have a direct impact on children's ability to participate in more complex floor exercise activities. Therefore, in the context of physical education, especially floor gymnastics activities, the lack of readiness in basic locomotor skills has the potential to hinder the achievement of learning outcomes targeted by the curriculum. This study aims to determine the relationship between basic locomotor skills and learning outcomes in floor gymnastics activities. The method used in this study is a quantitative descriptive method with a correlational technique. The population in this study were students at Public Junior High School 40 Bandung, specifically those in grade VII, with a total of 290 students. The sample in this study used simple random sampling, with samples taken randomly by lottery, namely VII-A, which consisted of 32 students. The instrument used in this study to measure basic locomotor skills was the Test of Gross Motor Development (TGMD-2), while for learning outcomes in floor exercises, the researcher used assessments of knowledge, skills, and attitudes. The results of data analysis showed that the correlation coefficient of variable X with Y (rxy) was 0.603, which was categorized as having a strong correlation level, namely in the range of 0.60-0.799. This value also indicated a positive relationship between basic locomotor skills and learning outcomes in floor exercises.
Three Paradigms for Learning Mathematics with the Aid of Artificial Intelligence: A Phenomenological Study of Prospective Teacher Students Syfa Nurfadilah; Gifa Nur Arofah; Toto Subroto; Tonah
International Journal of Educational Research Excellence (IJERE) Vol. 3 No. 2 (2024): July-December
Publisher : PT Inovasi Pratama Internasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55299/ijere.v3i2.923

Abstract

The 21st century has seen rapid changes in educational practices, mainly due to technological advancements such as artificial intelligence. Especially in today's digital age, technology is essential in transforming education. One prominent innovation is the use of AI in the context of learning. This research aims to discover prospective teachers' learning processes using AI. In addition, this research seeks to determine the position of AI in learning mathematics for prospective teachers. The participants are Mathematics Education students of Universitas Swadaya Gunung Jati (UGJ) Cirebon. This qualitative study uses phenomenological methods by collecting data from each research subject about experiences regarding the use of AI in solving several math problems. This research provides insight into how prospective teachers can utilize AI technology in the mathematics learning process. In addition, this research is essential because it contributes to the development of technology-based pedagogy, a significant trend in global education today. This research uses data collection methods through observation, interviews, and content. The results of this study are that 3 paradigms from previous research are in line with the theory of 3 paradigms: (1) AI-directed students as recipients, (2) AI-supported students as collaborators, and (3) AI-empowered students as leaders. Most participants in this study were collaborators in these three paradigms.
Implementing an Audiovisual-Based Flipped Learning Model to Increase Interest in Learning Pencak Silat Cindy Aulia Sari; Toto Subroto; Regi Dwi Septian
Journal of Physical Education Health and Sport Vol. 12 No. 2 (2025)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jpehs.v12i2.38475

Abstract

Interest in learning among students is a crucial element for achieving success in physical education, especially in pencak silat material which requires active participation from students. However, in learning pencak silat at the secondary school level, problems are often found such as low student interest and involvement, which is caused by traditional teaching methods. This research aims to increase students’ interest in learning through the use of a flipped learning model with the support of audiovisual technology media in learning pencak silat. This study uses the Classroom Action Research (CAR) approach developed by Kemmis and McTaggart, which was carried out in two cycles with the pre-cycle stage as the initial condition. The subjects in this research consisted of 32 class XII students at State Senior High School 1 Pabuaran. Data regarding student interest in learning was collected through a questionnaire adapted from Budiono (2012) and has been tested for validity and reliability. Data processing was carried out in a quantitative descriptive manner using the overall percentage of success. The findings of this research show a significant increase in students’ interest in learning, which rose from 38% in pre-cycle to 72% in cycle I and increased again to 91% in cycle II. The innovation of this research lies in combining the flipped learning model with audiovisual media which is applied in a structured manner in pencak silat learning at the secondary school level. These results indicate that the application of flipped learning supported by audiovisual technology is very effective in increasing students’ interest in learning pencak silat.  
Lokakarya Reflektif–Partisipatif untuk Penguatan Wawasan Kebinekaan Global Guru Sekolah Dasar Toto Subroto; Rudi Adi Nugroho; Asep Herry Hernawan; Abih Gumelar; Gymnastiar Nur Hambali; Yulia Puspita
Aksiologiya: Jurnal Pengabdian Kepada Masyarakat Vol 10 No 3 (2026): Agustus
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/aks.v10i3.28943

Abstract

Masyarakat dengan berbagai keragaman di dalamnya menyimpan potensi positif dan negatif, baik di skala nasional maupun global. Berbagai kasus di masyarakat menunjukkan potensi negatif yang semakin menguat, khususnya terkait kasus intoleransi, tidak terkecuali di dunia pendidikan. Guru yang merupakan elemen penting dalam dunia pendidikan perlu mendapatkan penguatan terkait wawasan kebinekaan global agar lebih mampu menangkal potensi intoleransi di dunia pendidikan, khususnya di sekolah. Lokakarya reflektif-partisipatif ini bertujuan untuk memberikan penguatan sekaligus bimbingan teknis bagi para guru SD terkait implementasi wawasan kebinekaan global di sekolah. Peserta kegiatan ini adalah guru-guru SD di Kecamatan Lembang, Kabupaten Bandung Barat. Kegiatan ini dilaksanakan secara luring di SDN Pancasila dan diikuti oleh 21 peserta. Kegiatan lokakarya terlaksana dengan efektif. Kegiatan dikemas dengan sangat interaktif dan peserta sangat antusias dalam mengikuti seluruh rangkaian kegiatan. Berdasarkan berbagai dokumen yang dihasilkan peserta dapat disimpulkan bahwa wawasan kebinekaan global para peserta meningkat yang dapat dilihat pada kemampuan peserta dalam mengaplikasikan konsep-konsep tentang kebinekaan global yang telah terima. Peserta menunjukkan kemampuan yang sangat baik dalam mengidentifikasi berbagai potensi di sekolah untuk menguatkan budaya toleransi di sekolah. Selain itu, peserta menunjukkan kemampuan dalam penyusunan rencana peguatan budaya toleransi terhadap berbagai keragaman di sekolah.
Co-Authors . Tonah Abdul Alim Kahari, Abdul Alim Abu Bakar M.I. Syihab Aceng Hilman Ade Rizki Ridwan Firdaus Agus Gumilar Ahmad Fariz Maulana Alifa I. Nabila Amiruddin, Mohd. Hasril Angga Himas Setyawan Anita Yuwita Anneke Noviyanti Aprillia, Mutiara Sukma Ari Hardianto Asep Herry Hernawan Bachti Alisjahbana Bacthi Alisjahbana Basti Andriyoko Bevi Lidya Bevi Lidya Bisri Musthofa Budi Santoso Budiana, Dian Budiantoro, Ogi Cindy Aulia Sari D Agus Yusuf Wildan Dani Permana Debby M Sumanti Defi Supriyati, Ayu DESSY NATALIA DESSY NATALIA DESSY NATALIA Desy Linasari Dewi Astriany Dewi Ratih Handayani Dhiya Salsabila Diana P Rahmawati Diana Rakhmawaty Eddy Didi Suryadi Didin Budiman, Didin DWI RAHMAWATI Dzakiyah 'Izzah Ghassani Adsumi Effendi, Syulastri EKO YULIANTO Elazmanawati Lembong Fadhilah, Muhammad Naufal Fadhlillah, Muhammad Fadillah, Lukky Farihandiandra, Yusron Fathurrohman, Muhammad Andi Fatin, Nuri Nuraini Fauzian Giansyah Febriani Aji Kusuma, Sherlynda FERNITA PUSPASARI Firdaus, Muhammad Lutfi Gifa Nur Arofah Gumelar, Abih Gumilar, Agus Gymnastiar Nur Hambali Hafizatunnisa, Hafizatunnisa Hambali, Burhan Hamdiyanti, Meti Henry Chandra Hesti Lina Wiraswati Idar Idar Idar Idar Idar, Idar Iman Permana Maksum Iman Rahayu Kharimah, Nurul Ikhsan Khomaini Hasan KHOMAINI HASAN Khomairi Hasan Korry Novitriani Korry Novitriani, Korry Kusumawardani, Shinta Kusumawardhani, Shinta Laelasari Laelasari , Laelasari Laelasari Laelasari Laelasari Laelasari, Laelasari Leonardus Wiydatmoko Lesmana, Gianjar Lukky Fadillah Mamlikatu Ilmi Azizah Marisa Novianti Fajrah Ilsya Meti, Meti Hamdiyanti Mony, Siti Rasnawati Muchamad Subali Noto Muhamad Abidin Muhamad Zulchoir Rasyid Muhammad Fadhil Z, Rusdi Noor Rosa (p:43-50) Muhammad Fadhlillah Muhammad Hilman Daniswara Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Muhammad Yusuf Murniaty Simorangkir Murniaty Simorangkir Naufal D, Kevin Nida Yumna Niko, Ade Nisa Fauziah Noviyanti, Anneke Nugroho, Rudi Adi Nurainy, Neni Nurfairuz, Hasna Siti Nurmalasari, Ratna Nurul Aida Fathya Nurul Ikhsan Karimah Nurul Ikhsan Karimah O Suprijana O Suprijana O Suprijana, O OGI BUDIANTORO Ogi Budiantoro Oo Suprijana Opik Taupiqurrohman Opik Taupiqurrohman Pertiwi, Wulan Praja, Ena Suhena Purba Upay Rahmaniar Mulyani Rahmaniar Mulyani Rahmat, Alit Ratna Yulistya Ningrum Regi Dwi Septian Riezki Amalia Rima Handiyani Risnina Wafiqoh Rizky Rosjanuardi Rochmat Rahayu Rodiana , Ivta Rodiana, Ivta Safri Ishmayana Safrl Ismayana Sahroni Sahroni Santhy Wyantuti Sari Agung, Syennie Sari Syahruni Sari Syahruni SARONOM SILABAN Saronom Silaban Saronom Silaban Saronom Silaban Setyaningrum, Sinta Shabarni Gaffar Shinta Kusumawardani Siti Nuraini Siti Soidah Siti Umayah Siti Umayah, Siti Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro Soetijoso Soemitro SOETIJOSO SOEMITRO Sofyan Multazam N Aji Sri Asnawati Sri Pitriayana Sriwidodo Sriwidodo Sriwidodo Sriwidodo Sukma Nuswantara Sukma Nuswantara Sukma Nuswantara Sunan M. Alpiansyah Surya Amami Pramuditya SUTARYA ENUS Sutarya Enus Sutarya Enus Sutarya Enus Syafrudin, Elza Nurrachmanita Syani, Mamay Syfa Nurfadilah Taupiqurrohman, Opik Taupiqurrohman, Opik Tika Pradnjaparamita, Tika Triana N Meirina Umi Baroroh Umi Baroroh Umi Baroroh, Umi Unang Supratman Upay, Purba wahyu widayat Wanda Destiarani WANGSA TIRTA ISMAYA Wildatus Sholihah Windy Aulia Putri Wulan Pertiwi Yani Suryani Yeni W Hartati Yeni Wahyuni Hartati Yosua Yosua Yulia Puspita Zakiyyah, Salma Nur