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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya El-Hayah : Jurnal Biologi Research Journal of Life Science Jurnal Teknologi Pertanian Berkala Kedokteran MNJ (Malang Neurology Journal) Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Majalah Kesehatan FKUB Health Notions Journal of Dentomaxillofacial Science Jurnal Informasi Kesehatan Indonesia (JIKI) Narra J Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery)
Hidayat Sujuti
Biochemistry Laboratory, Faculty Of Medicine, University Of Brawijaya Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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EFEK PAPARAN PROGESTERONE TERHADAP EKSPRESI TIROSIN HIDROKSILASE DAN AKTIFITAS LOKOMOTOR, SERTA PENGARUHNYA TERHADAP KADAR AROMATASE-B PADA EMBRIO ZEBRAFISH (Danio rerio) Hanif, Hanif; Soeharto, Setyawati; Sujuti, Hidayat
Majalah Kesehatan Vol. 6 No. 4 (2019): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.majalahkesehatan.2019.006.04.1

Abstract

Selain berperan dalam sistem reproduksi, progesterone juga mempunyai peran dalam sistem saraf. Progesterone diketahui berperan dalam proses perkembangan sel Purkinje pada tikus. Sel dopaminergik mempunyai peran penting dalam tubuh khususnya fisiologi pergerakan. Perubahan konsentrasi dopamin dapat berakibat pada timbulnya kelainan seperti Parkinson dan amyotropik lateral sklerosis. Pada zebrafish, sel dopaminergik dapat diidentifikasi dengan pengamatan pada tirosin hidroksilase dan pengamatan aktifitas pergerakan dapat dilakukan penilaian aktifitas lokomotor. Pemahaman terhadap hal ini akan membantu upaya terapi penyakit-penyakit neurodegeneratif terkait dopamin.Tujuan penelitian ini adalah membuktikan pengaruh progesterone pada sel saraf dopaminergik embrio zebrafish melalui pengamatan ekspresi tirosin hidroksilase, aktifitas lokomotor dan peran progesterone dalam regulasi estrogen melalui aromatase B. Sebanyak 30 embrio zebrafish dipelihara dan dikultur dalam cawan dengan terpapar progesterone pada medium dengan konsentrasi 0,1, 1, dan 10 µM. Penelitian diulang tiga kali menggunakan masa peneluran yang berbeda. Teknik imunositokimia dengan marker fluoresens digunakan untuk mengamati perubahan ekspresi tirosin hidroksilase pada diensefalon saat 48 hpf. Pengukuran aktifitas lokomotor dilakukan dengan kuantifikasi gerakan larva selama 1 menit pada usia 144 hpf. Ekspresi mRNA aromatase B dinilai dengan metode RT-PCR menggunakan primer Arom-B pada usia 48, 72, dan 96 hpf. Analisis statistik menggunakan ANOVA dengan nilai p < 0,05. Progesterone menurunkan ekspresi tirosin hidroksilase pada daerah diensefalon embrio zebrafish. Progesterone terbukti menurunkan aktifitas lokomotor larva. Paparan progesterone menyebabkan peningkatan ekspresi mRNA aromatase B terutama pada usia 72 hpf dan 96 hpf. Kesimpulannya, progesterone dapat mempengaruhi sel dopaminergik embrio zebrafish melalui penekanan ekspresi tirosin hidroksilase, aktifitas lokomotor, dan meningkatkan ekspresi mRNA aromatase B.
Possible Role of Akt in Mossy Fiber Sprouting: Akt Activity and CA3 Mossy Fiber Sprouting in a Kainate Model of Epilepsy: Akt Activity and CA3 Mossy Fiber Sprouting in Epilepsy husna, Machlusil; Handono, Kusworini; Sujuti, Hidayat; Aulanni'am, Aulanni'am
Journal of Tropical Life Science Vol. 14 No. 3 (2024): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.09

Abstract

The most prevalent pathological phenomenon observed in patients with epilepsy is hippocampal mossy fiber sprouting (MFS), which is thought to be associated with epileptic progression, such as worsening seizure control, cognitive function, and behavior. MFS is discovered in the dentate gyrus and the hippocampal Cornu Ammon 3 (CA3) area. The CA3 area is involved in memory, so disturbances in that area can affect memory impairment in patients with epilepsy. The mammalian target of rapamycin complex-1 (mTORC1) is also associated with MFS. Akt is an upstream activator of mTORC1 and a downstream target of mammalian target of rapamycin complex-2 (mTORC2) and plays a role in cytoskeleton organization. We analyzed Akt activity and MFS in the CA3 zone in an in vitro model of kainate-induced epilepsy. We divided organotypic hippocampal slice cultures (OHSCs) into a kainate (epilepsy) group and a control (untreated) group. On the 10th day in vitro (DIV), the kainate group was exposed to 8 µM kainic acid for 48 h, diluted in the medium. At 32 DIV, we measured Akt activity through western blotting and CA3 MFS through synaptoporin fluorescence intensity observed using confocal laser scanning microscopy. We found that Akt activity increased significantly (p = 0.000) in the kainate group, and the synaptoporin fluorescence intensity also increased in the stratum oriens of the CA3 area (p = 0.049) in the kainate group. Our findings implied that Akt may play a role in MFS development. Because Akt is a main downstream target of mTORC2, mTORC2 may also be involved in MFS development. Further research is required to clarify these findings.
Effect of Histatin-1 Proteins to Fibroblasts Growth Factor-2 (FGF2) Concentrate and Fibroblasts Cell Migration on Human Gingival Fibroblast (HGF) Culture Dewi, Ni Ketut Ayu Feriyanti; Permatasari, Nur; Sujuti, Hidayat
Research Journal of Life Science Vol 8, No 2 (2021)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2021.008.02.4

Abstract

The purpose of this study is to determine the effect of histatin-1 protein increasing the concentration of fibroblast growth factor (FGF2) and fibroblast cell migration in human gingival fibroblast (HGF) culture. The HGF cell culture used was obtained from odontectomy patients and was the result of the 4th passage. Cell culture was divided into 4 groups: HGF control group (without treatment) and treatment groups with 5µM, 10µM, and 20µM concentration of histatin-1 respectively. The amount of fibroblast growth factor 2 (FGF2) was quantified by using ELISA method. In vitro fibroblast cells migration was measured using scratch/wound healing assay method. FGF2 concentration and HGF cell migration were measured at hour 0, 3, 11, 24, and 48. The results of the One Way Anova statistical test difference between FGF 2 (ΔFGF2) at hour 24 showed a value of P = 0.042 (P≤0.05) and HGF cell migration at hour 11 showed a P value = 0.003 (P≤0.05). The conclusion of this study is that Histatin-1 Protein is able to increase the concentration of Fibroblast Growth Factor 2 (FGF2) and the migration of Human Gingival Fibroblast cells.
Possible Role of Akt in Mossy Fiber Sprouting: Akt Activity and CA3 Mossy Fiber Sprouting in a Kainate Model of Epilepsy: Akt Activity and CA3 Mossy Fiber Sprouting in Epilepsy husna, Machlusil; Handono, Kusworini; Sujuti, Hidayat; Aulanni'am, Aulanni'am
Journal of Tropical Life Science Vol. 14 No. 3 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.03.09

Abstract

The most prevalent pathological phenomenon observed in patients with epilepsy is hippocampal mossy fiber sprouting (MFS), which is thought to be associated with epileptic progression, such as worsening seizure control, cognitive function, and behavior. MFS is discovered in the dentate gyrus and the hippocampal Cornu Ammon 3 (CA3) area. The CA3 area is involved in memory, so disturbances in that area can affect memory impairment in patients with epilepsy. The mammalian target of rapamycin complex-1 (mTORC1) is also associated with MFS. Akt is an upstream activator of mTORC1 and a downstream target of mammalian target of rapamycin complex-2 (mTORC2) and plays a role in cytoskeleton organization. We analyzed Akt activity and MFS in the CA3 zone in an in vitro model of kainate-induced epilepsy. We divided organotypic hippocampal slice cultures (OHSCs) into a kainate (epilepsy) group and a control (untreated) group. On the 10th day in vitro (DIV), the kainate group was exposed to 8 µM kainic acid for 48 h, diluted in the medium. At 32 DIV, we measured Akt activity through western blotting and CA3 MFS through synaptoporin fluorescence intensity observed using confocal laser scanning microscopy. We found that Akt activity increased significantly (p = 0.000) in the kainate group, and the synaptoporin fluorescence intensity also increased in the stratum oriens of the CA3 area (p = 0.049) in the kainate group. Our findings implied that Akt may play a role in MFS development. Because Akt is a main downstream target of mTORC2, mTORC2 may also be involved in MFS development. Further research is required to clarify these findings.
Pengaruh Pemberian Extra Virgin Olive Oil terhadap Jumlah Sel Granulosa dan Kadar 17 β Estradiol Tikus yang di Papar Rhodamin B Setiyaningsih, Fera Yuli; Dwi Jayasa, Pande Made; Sujuti, Hidayat; Retnani, Diah Prabawati
Journal of Ners and Midwifery Vol 5 No 3 (2018)
Publisher : STIKes Patria Husada Blitar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26699/jnk.v5i3.ART.p241-248

Abstract

The use of dyes in food today is very apprehensive. Many foods contain dyes that are prohibited to be used in food. The addition of the dye will have an impact on health. The aim of this research was to know the effectiveness of the administration of extra virgin olive oil to the number of granulosa cell and 17β estradiol level of Rats exposed by rhodamin B. The design of this study was true experimental with post test approach of group design control. The subject of the study was 30 white mice Rattus novergicus in the age of 12 weeks weight average 220 grams and in healthy condition. Rats were divided into 5 groups. Control group without treatment, positive control treatment with rhodamine B 18 mg / 200gr. Treatment 1 administration of rhodamine B 18 mg / 200gr and EVOO (Extra Virgin Olive Oil) 1.5 ml / KgBB, treatment 2 rhodamine B 18 mg / 200gr and EVOO 3 ml / KgBB, treatment 3 rhodamine B 18 mg / 200gr and EVOO 4,5 ml / KgBB. The number of granulosa cells was determined by HE staining, the level of 17β estradiol with ELISA. The data was analyzed by Kruskal Wallis test, Spearman Rank and Simple Linear Regression with degree of significance α <0.05. The average number of granulosa cells and the lowest levels of 17β estradiol were found in the positive control group and the highest average was in the treatment group 3. There was one dose that could significantly increase granulosa cells and the beta estradiol levels compared with the treatment group the other with niali α <0.05. The administration of EVOO affected the number of granulosa cells and the levels of 17β estradiol in rats exposed by rhodamine B.
Colchicine attenuates chemical hypoxia-induced pyroptosis through downregulation of nuclear factor kappa B and caspase-1 in cardiomyocytes Satrijo, Budi; Rohman, Mohammad S.; Aulanni'am, Aulanni'am; Sujuti, Hidayat; Lestari, Bayu
Narra J Vol. 5 No. 2 (2025): August 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i2.2245

Abstract

Myocardial infarction (MI) is the leading cause of mortality worldwide. During MI, cardiomyocyte necrosis and inflammation are crucial in the post-MI cardiac remodeling process, including pyroptosis. Although colchicine is a well-known anti-inflammatory drug that has been clinically studied in the context of MI, its role in cardiac pyroptosis remains unclear. The aim of this study was to investigate the role of colchicine in pyroptosis in vitro, using CoCl2-induced H9c2 cells. Prior to the primary experiment, the hypoxic model in H9c2 cells was optimized by evaluating hypoxia-inducible factor-1 alpha (HIF-1α) expression and viability in cells exposed to various concentrations of CoCl2 at different time intervals. Subsequently, an in vitro hypoxia model was established by treating H9c2 cells with CoCl2 (600 µM), with or without colchicine (1 µM), for 3 hours. Flow cytometry was used to measure the expression of nuclear factor-kappa beta (NF-κB), interleukin 18 (IL-18), caspase-1, and HIF-1α in pyroptotic cells. Immunofluorescence was used to assess caspase-1 localization and its colocalization with propidium iodide during late-stage pyroptosis. Our data indicated that CoCl2-induced hypoxia significantly upregulated NF-κB, caspase-1, and IL-18 expression, and increased pyroptotic cell death in H9c2 cells. Colchicine treatment attenuated these effects, leading to a marked reduction in NF-κB, caspase-1, and IL-18 expression in hypoxic cells. Colchicine treatment significantly decreased the number of late pyroptotic cells. The protective effect of colchicine was more pronounced in late hypoxia (24-hour) setting compared to early hypoxia (3-hour). These findings suggest that colchicine attenuates cardiac pyroptosis in hypoxic H9c2 cells, as evidenced by the significant downregulation of key proteins involved in this pathway, including NF-κB, caspase-1, and IL-18. This protective effect appeared to be more effective in late hypoxia.
Kombucha Based on Javanese Turmeric (Cucurma zanthorrhiza and Cucurma zedoaria): Effect of Various Concentrations and Antioxidant Activity Zubaidah, Elok; Kusmiyati, Nur; Arum, Mentari Sekar; Hasfiani, Yuliatin; Sujuti, Hidayat
El-Hayah:Jurnal Biologi Vol 10, No 2 (2025): EL-HAYAH (VOL 10, NO 2 Maret 2025)
Publisher : Program Study of Biology, Science and Technology Faculty, UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/elha.v10i2.35542

Abstract

Temulawak (C.zanthoriza) and white turmeric  (C.zedoaria) are Indonesian herbal plants  widely used to treat various diseases including anti-inflammatory properties, neuroprotective, nephroprotective, antitumor, and hepatoprotective. A long-standing tradition, Kombucha is a drink produced by fermenting tea using combine of lactic acid bacteria, acetic acid bacteria and mold.  It's classified as a functional food due to its high antioxidant and antibacterial activity, suggesting potential benefits for disease prevention.This research aims to improve the functional value of kombucha by using temulawak and white turmeric. Kombucha is made from temulawak and white turmeric powder with various concentrations ranges 0.2 – 1% (w/v)) and a sugar concentration of 10% (v/v)). After 8 days of fermentation, temulawak and white turmeric kombucha were tested for pH, total acid, amount of phenol, and IC50. The effect of various concentrations temulawak and white turmeric as a substrate for kombucha demonstrated significant (p0.05) in reduction of pH, increased total phenol content, IC50 value of antioxidant activity, also increased total acid. The best treatment of temulawak and white turmeric kombucha was obtained at a concentration of 1% with a pH of 2.77 for temulawak kombucha and 2.80 for white turmeric kombucha; total acid of 4.38% for temulawak kombucha and 3.60% for white turmeric kombucha; total phenols were 232.48 mgGAE/ml for temulawak kombucha and 113.00 mgGAE/ml for white turmeric kombucha; The IC50 value was 30.82 ppm for temulawak kombucha and 40.15 ppm for white turmeric kombucha.
EFFECT OF HYDROCOLLOID TYPE AND GINGER EXTRACT ON THE PROPERTIES OF RED BEETROOT JELLY CANDY (Beta Vulgaris L.). Fitriah, Arsinah Habibah; Gading, Inges Manggar; Tri Dewanti Widyaningsih; Wulan, Siti Narsito; Sujuti, Hidayat
Jurnal Teknologi Pertanian Vol. 26 No. 2 (2025)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtp.2025.026.02.7

Abstract

Jelly candy is a type of soft candy made with the addition of hydrocolloids. Currently, jelly candies are widely made with ingredients that can enhance their nutritional and functional value. Red beet, a material rich in bioactive compounds, can be used as a raw material for making jelly candy. Processing red beet into jelly candy may increase its acceptance due to reduced off-flavors. This study aimed to determine the effect of different types of hydrocolloids and the addition of ginger extract on the physicochemical and organoleptic properties of red beet jelly candy. The research method used was a nested design with three replications. The red beet jelly candy was made with different types of hydrocolloid (agar and carrageenan) and different concentrations of ginger extract (0%, 1%, and 2%). The results showed that the difference in the type of hydrocolloid significantly affected the ash content, nitrate and nitrite content, total phenol, and total betalain content of the product. While the addition of ginger extract nested in the type of hydrocolloid had a significant effect on the a* value, antioxidant activity, total phenol, and total betalain content of the product. The best treatment was determined using the Zeleny method. Data processing results showed that the best treatment obtained was agar jelly candy with the addition of 0% ginger extract, with an antioxidant activity value of 530.15 ppm.
Co-Authors A. Harijono A.A. Ketut Agung Cahyawan W Abdalrauf A Mahmud Yousif Abdalrauf A Mahmud Yousif, Abdalrauf A Mahmud Abdussalam Ashour, Abdussalam Achmad Rudijanto Adinda Chika Anindita Affandi, Haykal Agung Prasetyo Wibowo Agustiana Dwi Agustiana Dwi Indiah Ventiyaningsih Agustina Tri Endharti Ala Shukri Eshami, Ala Shukri Analis W Wardhana Anik Puryatni Anisak, Siti Ariani Ariani Ariani Ariani Arifin, Mochammad Samsul Arsinah Habibah Fitriah, Arsinah Habibah Arum, Mentari Sekar Askandar Tjokroprawiro Astika Swastirani Aulanni'am, Aulanni'am Aulia Yasmin, Aulia Bagus Hermansyah Bambang Rahardjo Bayu Kurniawan Bintang Soetjahjo Bogi Pratomo Wibowo Burhan, Niniek Chabibi, Mochamad Chabibi Chairinnisa, Elsa Safira Christina Dewi Dalhar, Mochamad Dewi, Ni Ketut Ayu Feriyanti Dian Handayani Dian Nugrahenny Diana Lyrawati Diana Lyrawati Dicky Faizal Irnandi Didi Candradikusuma Didi Candradikusuma Dr.Ir. Yunianta, DEA Dwi Jayasa, Pande Made Dwi Jayasa, Pande Made Dwi Yuni Nur Hidayati Edi Mustamsir Edi Widjajanto Edi Widjajanto Edwin Widodo Effendi, Ma'sum Eko Sulistijono Elok Zubaidah Erma Sulistyaningsih Ery Olivianto Fardizia Putri Alia Feni Istikharoh Fibrianto, Yuda Fita Shofiyah Gading, Inges Manggar Gerry Gunawan, Gerry H., Nunuk Handono Kalim Hanif Hanif Happy Kurnia Permatasari Hara, Kenta HARI PURNOMO Hariwati Hariwati Hasfiani, Yuliatin Huda Rohmawati Husna, Ully Husnul Khotimah Ika Kustiyah Oktaviyanti Indrayanti Indrayanti Indriati Dwi Rahayu Inggita Kusumastuty Josef Sem Berth Tuda Juniartha, Putu Kalsumy, Umi Kanthi Permaningtyas Tritisari, Kanthi Permaningtyas Karyono Mintaroem Karyono Mintaroem Karyono Mintaroem Karyono mitaroem, Karyono Krisni Subandiyah Krisni Subandiyah Kusworini Handono Laily Yuliatun Lavina Sofia Ardani Lely Retno W Leny Budhi Harti Lestari, Bayu Lirawati, Diana Loeki Enggar Fitri M Rasjad Indra Machlusil Husna, Machlusil Maimun Z Arthamin, Maimun Z Masafumi Matsuo Masdar Muid Masdar Muid Masruroh Rahayu Masruroh Rahayu, Masruroh Mayangsari, Elly Miftahu Soleh Moch. Aris Widodo, Moch. Aris Mochammad Dalhar Mohammad Hidayat Mohammad Hidayat Mohammad S. Rohman Muhammad Ali Faisal Muhammad Rasjad Indra Muhammad Rasjad Indra Nabila Andjani, Nabila Nabila Erina Erwan Nashi Widodo Nathania Bella Claresta Niarti Ulan Sari Siarnu Nindy Sabrina Niniek Burhan Nisrina Nur Afina, Nisrina Nur Nur Kusmiyati Nur Permatasari Nura Fattah Cantika Yoga Nurshalilah Nurshalilah Pande Made Dwi Jayasa Pande Made Dwijayasa Prima Wijayanti, Prima Primawardani, Putri Puryatni, Anik - Putri, Dianita Setya Pradita Putu Juniartha Raisa, Neila Raudhatul Jannah Restuningwiyani, Sintha Retnani, Diah Prabawati Retty Ratnawati Retty Ratnawati Rizky Amalia RR. Ettie Rukmigarsari Salim, Irfan Agus Samsul Arifin Santoso, Giovani Anggasta Satrijo, Budi Selvi Marcellia Septi Nur Rachmawati Setiyaningsih, Fera Yuli Setyawati Soeharto Setyawati Soeharto, Setyawati Shintiya Dewi, Debby Shofiyah, Fita SIMON BAMBANG WIDJANARKO Sintha Restuningwiyani Siti Lintang Kawuryan Siti Narsito Wulan Soetjahjo, Bintang Sri Andarini Sutrisno Sutrisno Syahrul Chilmi, Syahrul Syarifah Rohaya Tatit Nurseta Tatit Nurseta Tatit Nurseta Tika Ardhini Wardoyo Tinny Endang Tinny Endang Hernowati Tokunaga, Chiharu Tri Dewanti Widyaningsih Triawanti Triawanti Tuda, Josef Sem Berth Umar Zein Vierlia, Wino Vrieda Widowati, Hesty Widya Rahmawati Winda Nurtika Yonezawa, Kazuyoshi Yoshino, Ken-ichi Yuda Fibrianto Yudanto, Hendy Setyo Zuly Vita Aulya, Zuly Vita