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Jurusan Teknik Kimia, Fakultas Teknologi Industri Universitas Katolik Parahyangan
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Journal : JURNAL FARMASIMED (JFM)

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Isolation and Structural Analysis of Active Antioxidant Phenolic Compounds from Elephantopus mollis Kunth Verawati, Verawati; Almahdy, Almahdy; Febriyenti, Febriyenti; Prima Putra, Deddi
Jurnal FARMASIMED (JFM) Vol 8 No 1 (2025): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/a2hcyr24

Abstract

Elephantopus mollis is a common weed found in West Sumatra, known for its diverse traditional uses and rich phytochemical content. This study aimed to isolate phenolic compounds and evaluate the antioxidant activity of the polar extract of E. mollis herb. The polar extract was obtained through successive extraction using solvents of increasing polarity. Separation of the polar extract was carried out using Amberlite XAD-4 resin chromatography with methanol and water eluents, followed by isolation through silica gel column chromatography using a step-gradient polarity elution system. The separation and isolation processes were monitored by thin-layer chromatography (TLC). Total phenolic content was determined by the Folin–Ciocalteu method, while antioxidant activity was assessed using the DPPH radical scavenging assay. Structural identification of the isolated compound was performed using UV-Vis, IR, NMR, and LC-MS spectroscopic techniques. Subfraction D of the polar extract exhibited a high phenolic content of 618.06 ± 3.38 mg GAE /g. From subfraction D, a phenolic compound designated D3a was isolated as a yellowish-white powder (95.30 mg) with an Rf value of 0.64, producing a dark blue color upon reaction with 10% FeCl₃. The antioxidant activity of compound D3a was classified as very strong, with an IC₅₀ value of 12.42 ppm. Based on physicochemical characterization, compound D3a was identified as caffeic acid with a molecular formula of C₉H₈O₄ and a molecular weight of 180.049.
Co-Authors Abbas, Fhaisal Fernanda Abidin, Urwatil Wusqa Ade Salahudin Permadi Ady Purwoto Agung Nur Cahyanta, Agung Nur Agus Salim Ahmad Yani T Alkarimah, Khairani Almahdy A Andriana Anela Putri, Suci Apriani, Nining Baso Amri Borut, Mohamad Caroline, Shinta Cheng Jinkuan, Cheng Jinkuan Comalasari, Enny Desprayoga, Desprayoga Dian Nugraha Didi Susianto Didin Erma Indahyani Dito Anurogo, Dito Dwi Perwitasari Wiryaningtyas, Dwi Perwitasari Dwi Rahmawati, Agustini Liviana Ekawati, Nur Elfionita, Yolla Sarni Elmiati Elmiati Elvira Junita Endang Sri Suyati Estralita Trisnawati Fachry Abda El Rahman Fahlevie, Rinaldi Agusta Fauzi, Sellia Felia Susanto, Marcella Felicia, Elsa Fendri, Sandra Tri Juli Fera, Okta Fitriani, Helda Fitriani, Helda Florencia, Silviana Gunawan, Awal Hafid, Istiqamah Haiqal, Andi Fikri Happy Fitria, Happy Harapan, Edi Hartono Hartono Haryadi - Haulussy, Rais Rahman Hera, Hera Herni Kurniawati Husni Mubarok Ibni Hadjar Iin Nurbudiyani Ilham Ilham Ilham Ilham Indra Perdana Irnawati Irnawati Irwandi Irwandi Isnovian, Isnovian istiqomah istiqomah Izzata Barid jannah, nurmiftahul Jap, Yustina Peniyanti Jinkuan, Cheng Jonathan, Stevanus Kadir, Erni Kornela, Andhini Kune, Syarifuddin Larasati Larasati Liliandriani, Andi Lukman, Hendro Lukmanulhakim ., Lukmanulhakim M. Fatchurahman Maharani, Bernadetta Diansepti Mareta Akhriansyah Masita, Syarifah Metio Alberto MS, Nur Hidayah Muh Risal MUHAMMAD HERMAN Muhammad Naufal Hakim MUSOLI, MUSOLI Mustaruddin Mustika, Uray Ndaru Nandhita, Felim Alzena Ni'mah, Nurun Nopryna, Nopryna Novita Nining Anggraini Nuriza Agustina Nurlina Nurlina Nurwahidin, Muhammad Oei, Edy Oktoriyadi, Adri Fernando Pannyiwi, Rahmat Pratama, Muhammad Anzar Pricillia, Fanny Prima Putra, Deddi Priscilla, Priscilla Putri Dwi Cahyani, Putri Dwi Putri Irianti Sintaman Putri, Anggraini Raden Surahmat Ratih Sari Wardani Reni Astika, Reni Ria Afrianti Rieuwpassa, Sarmalina Riswandi Riswandi Romy Wahyuni Ruaida Ruaida Rusi Sahiruddin Sahiruddin Sahuddin, Muhammad sahuddin, Muhammad Sahuddin Shinta, Dewi Yudiana Siregar, Maralottung Sisca Naftali Siska Ferilda Siti Arnisyah Siti Juhairiah Siti Mardasela Suat, Hatty Suat, Rahmawati Sugiarto, Claudia Suharman, Muh Yahya Sulaimawan, Deddy Sulyanto, Pranandang Suryani Musi Syahra, Ulfa Syaiful Bachri Tri Dhevi Afiani Nggego Ummainyah, Ummainyah Valencia, Valencia Verawati, Aunurrahman Widyastuti Widyastuti Wijaya, Pani Ade Wirnelis Syarif Yuli Syafitri, Yuli Yuliana Yuliana Yulis Jamiah Zaunit, Muthia Miranda Ziaurrahman, Muhammad