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Journal : bioeksperimen

 1 
Assessment of genetic diversity in EMS-induced porang (Amorphophallus muelleri Blume) revealed by RAPD markers Haritzah, Rendha Maulana; Radita, Novia Fitri; Ramadanti, Isma Salsabila; Azzahro, Yusri Despa; Wahyudi, Didik; Resmisari, Ruri Siti; Suyono
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.15768

Abstract

Increasing genetic diversity of porang through mutation is urgently needed since porang has low genetic diveristy. Therefore, this study aims to increase the genetic diversity of porang using ethyl methanesulfonate (EMS) and analyze the genetic diversity of porang using RAPD markers. Porang explants were subjected to in vitro EMS induction at various concentrations (control, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%) and evaluated after 35 days. DNA amplification was carried out using 20 RAPD primers. Genetic diversity within and between porang populations was analyzed using POPGENE software, while cluster analysis was performed using PAST software to identify genetic diversity patterns. The results showed that EMS mutation induction effectively increased the genetic diversity of porang. Primers OPA-2 and OPA-9 were identified as the most effective primers for detecting genetic diversity resulting from mutation induction. The 0.2% EMS treatment is recommended because it produced the most notable genetic changes compared to the control and shows potential for generating superior mutants to support porang breeding programs.
Co-Authors Achmad Mahrus Helmi Admin JPA Aji, Samudra Alim, Nurmaranti AMALIA PUTRI, DIYAH Amirudin Andi Anggraeni Maharbid, Dian Anjasmara, Arinta Antina Delhita Aqilla Salma, Sefia Assyfa Ramadhina Auria Yusrin Fathya Ayu Silvia Dewi Azzahro, Yusri Despa Bagus Sumargo Balqis Khairunisa Frisyanto Bambang Sugiarto Budi Santosa Cecilia Chailla Sabrina Dania Siregar Della Oktaviani Putri Desi Dika Sari Desy Fitriani Dian Handayani Dian Novita Didik Wahyudi Dina, Karina Farkha Diskon Sitinjak Dwi Ratna Sari Dyah Kirani Noviyana Erwin Sutantyo Faizah Tasya Nabila FAIZAH, ISMI Farnila, Vera Faroh Ladayya Fatriyoko, Swi Juli Angga Firman Syarif Ibrohim Halimatus Sya’diyah Haritzah, Rendha Maulana I GUST MADE SANJAYA I Wayan Arsana Idham Ali Rosadi Ienne Yoseria Putri Ignatius, Jimmy Intan Sari Rufiana Iswati Ivan Adi Susanto Izwan Budiana Jaisy Aulia Juwarlan Kusnandar LAILY ROHMAWATI Leny Yuanita Lukman El Hakim M. Hudri Made Ardite Umbare Made Veronikawati Mahendra, Yusril Maulida Audia Firdaus Mauludyah, Rohmatul Maya Karnia Anggraini Meisin Nabilah Mimelientesa Irman Muhammad Rafli MULYADI Nadira Aulia Rizqiana Putri Narto Nauval Rahmad Salam Ngatmin Nicholas Renaldo Nikhmatul Yulistyarni, Anggi Nilam Novita Sari Ninik Umi Hartanti Noradina Nova Susana Nugroho, Kris Nurdin Nugraha Nurjanah Nurjannah Pawartani, Transita PRABOWO Prijo Harsono Putri Salsabilah Putri, Novita Yulia Putu Sudarma Radeni Sukma Indra Dewi Radita, Novia Fitri Rahayu, Winanjar Rahmi Fadiah Nasution Ramadanti, Isma Salsabila Ria Arafiyah Risma Widia Ramadhanti Rizaldi Putra Rizaldi Putra Rosanti Diah Safitri Ruri Siti Resmisari Rusbandi Sabri Sanjaya, Muhammad Fahyu Sean Nabilah Apsarini Sinta Rahmadani Siregar, Dania Siti Chasani Siti Rohmah Rohimah Slamet Arifin Soeprijanto Sri Mulatsih Sri Mulatsih Sri Mulatsih, Sri Mulyani Sugiarti Suhardjo Suharti Surapti, Fitri Susi Dewiasih Kusumawati Sutaman Tarsono Tian Abdul Aziz Tita Diah Alia Putri Tjahjana, Dominicus Josephus Swanto Tri Murdiyanto TRISNIA NUUR FADILAH, REISMA Umi Hartanti, Ninik Vera Maya Santi Vina Dwi Pratiwi Wahyu Ari Putranto Wahyudi Winarjo, Wahyudi Wati, Yenny Widyanti Rahayu Windi Oktaviani Windi Yuni Astutik Windyastuti, Dwi Wullida Hayuning Bidari YUNI SETYANINGRUM, EVIA Zahra Maulida Zahrah Hashifah