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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Biotechnology Widyariset Omni-Akuatika Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Indonesian Journal of Tropical Aquatic Widyariset Qadauna: Jurnal Ilmiah Mahasiswa Hukum Keluarga Islam Jurnal Media Akuakultur Indonesia Jurnal Pengabdian Inovasi dan Teknologi Kepada Masyarakat Jurnal Kedokteran Hewan International Journal of Educational and Life Sciences (IJELS) Journal of Fisheries & Marine Biodiversitas Journal of Biological Diversity

Alimuddin

Department Of Aquaculture, Faculty Of Fisheries And Marine Science, IPB University, Bogor, West Java, Indonesia

Author-ID : 2471926

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BIOCHEMICAL RESPONSES AND GENETIC EXPRESSIONS OF SYNTHETIC COMMON CARP POPULATIONS EXPOSED TO HIGH-AMMONIA REARING ENVIRONMENT yogi Himawan; Alimuddin Alimuddin; Kukuh Nirmala; Imron Imron; Joni Haryadi
Indonesian Aquaculture Journal Vol 16, No 1 (2021): (June, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.1.2021.13-19

Carp is one of the leading freshwater aquaculture commodities in Indonesia. Further improvement of carp strains by the Research Institute for Fish Breeding (RIFB), Sukamandi, Indonesia, has produced a synthetic F2 carp. The strain is assembled from different strains of carps and has shown better growth and health characteristics. Considering that high environmental ammonia (HEA) has affected most carp grow-out systems, this study aimed to determine the performance of the synthetic carp populations in a high ammonia rearing environment. The treatments were rearing media of the synthetic carp seed populations added with and without (control) 200 mg/L NH4Cl arranged in three replicates. A total of 30 fish seeds/aquarium, weighed 10-15 g/fish, was used in the study. Dissolved oxygen levels were maintained above 2 mg/L using aeration. This study shows that higher tolerant carp populations had red blood cells of 232.66 ± 17.24 cells/mL, indicating a direct effect of high ammonia on red blood cell count (p<0.05). Cortisol levels of 80.90 ± 6.35 ng/mL in resistant carp indicate significant differences (p<0.05). The relative expressions of the HSP70 gene in the liver (Log10) ranged between 0.72 and 2.80. The values demonstrate that high ammonia-resistant synthetic carp have a higher relative expression ratio of the HSP70 gene than the less resistant group. This research concluded that the populations of synthetic F2 carp showed a degree of resistance against high-ammonia rearing conditions. When it is ready for aquaculture, this synthetic carp strain could be farmed in high density using intensive systems in HEA-affected artificial lakes and reservoir

OVARY DEVELOPMENT, FSH AND LH GENES EXPRESSION OF INDONESIAN LEAFFISH, Pristolepis grootii (Bleeker, 1852), INJECTED WITH LUTEINIZING HORMONE-RELEASING HORMONE ANALOG Muslim Muslim; Agus Oman Sudrajat; Muhammad Zairin Jr.; Muhammad Agus Suprayudi; Arief Boediono; Iis Diatin; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 16, No 2 (2021): (December, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.2.2021.69-77

Indonesian leaffish, Pristolepis grootii (Bleeker, 1852), is an undomesticated freshwater fish species native to the rivers, flooded swamps, and tributaries of Indonesia. The fish is mainly captured for consumption. In order to prevent its extinction and supply its growing demands, the artificial breeding of the fish should be developed. The purpose of this study was to determine the optimum dose of luteinizing hormone-releasing hormone analog (LHRHa) for stimulating the female P. grootii gonadal development at a dosage of 0, 1, 10, and 50 µg kg-1 of fish. Female fish (20.0 ± 0.6 g) were adapted for 30 days in the rearing environment and then separated into 12 aquariums with six fish per aquarium. Fish were then reared for another 21 days and fed with Tubifex sp. The LHRHa injection was conducted twice on day-7 and 14. Fish bodyweight, gonadosomatic index, gonad histology, blood estradiol-17â, and FSH-â and LH-â gene expression were evaluated at day 0, 7, 14, and 21. The results showed that the injection of the LHRHa hormone stimulated the development of fish gonads and was better achieved with a higher concentration of LHRHa. The best treatment was observed by the administration of 50 µg kg-1 of LHRHa that produced the fastest development among all treatments. This study demonstrated that the LHRHa induction could potentially stimulate the gonadal development of the newly domesticated fish. To our knowledge, this is the first study that reported the success of the induction of female gonad development in the Indonesian leaffish P. grooti.KEYWORDS:

EFFICACY OF GP-11 KHV DNA VACCINE IN Cyprinus carpio koi Sekar Ayu Chairunnisa; Sri Nuryati; Alimuddin Alimuddin; Sri Murtini; Ayi Santika; Dwi Hany Yanti
Indonesian Aquaculture Journal Vol 11, No 1 (2016): (June 2016)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Koi herpesvirus (KHV) is one of the major pathogen for koi and common carp which cause high mortality and economic losses for the farmer. The purpose of this study was to determine the efficacy of glycoprotein-11 (GP-11) KHV DNA vaccine and compared to GP-25 KHV DNA vaccine. The vaccine in the form of naked DNA plasmidwas delivered by intramuscularly injection to the 3-month-old koi. The fish were divided into six groups, i.e. unvaccinated group (negative control C- and positive control C+), and vaccinated group (2.5 μg/100 μl of GP-11 (group 1), 7.5 μg/100 μl of GP-11 (group 2), 12.5 μg/100 μl of GP-11 (group 3), and 12.5 μg/100 μl of GP-25 (group 4)). At day 42 post vaccination, all fish of each groups were challenged by injecting KHV titre 10-3 FID50. Number of dead fish was counted everyday after the challenge until 30 days. The results showed that vaccinated fish were had survival rate of 83.33-93.33% (group 2, 3 and 4). It’s show that GP-11 KHV DNA vaccine has high efficacy. As conclusion, GP-11 DNA vaccine could be an alternative DNA vaccine for preventing KHV infection.

CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798) Andi Parenrengi; Alimuddin Alimuddin; Andi Tenriulo
Indonesian Aquaculture Journal Vol 12, No 2 (2017): (December, 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

White spot syndrome virus (WSSV) has caused mass mortality on tiger shrimp (Penaeus monodon) culture and adversely affects prawn industry worldwide including Indonesia. It is well known that the protein structure of WSSV plays an important role in the virus infection and morphogenesis process. A viral protein structure called VP-15 is located in the nucleocapsid of virion virus. The protein structure involves in the life cycle of WSSV in host cells. A gene encoding VP-15 could be involved in constructing the RNA interference (RNAi), so it is needed to isolate and characterize for RNAi technology purpose. The study was aimed to isolate and characterize the VP-15 from the infected WSSV tiger shrimp. The characterization of VP-15 was undertaken through assessment of nucleotide sequence, amino acid deduction, alignment nucleotide/protein searches using Genetyx and BLAST program, and dendrogram construction analysis. The results showed that VP-15 was successfully isolated in form of ORFDNA with a fragment size of 243 bp. The phylogenetic tree analysis revealed three clusters corresponding to the time (year) of isolates collection. The VP-15 consisted of 80 amino acids, two start codons (ATG), one stop codon (TAA), and one Kozak context (AAAATGG). Hydrophilic amino acid was the highest composition (44.2%), followed by neutral (31.2%) and hydrophobic (24.6%) amino acid groups. The VP-15 was rich in amino acid of lysine (21.3%), arginine (22.9%) and serine (24.6%). The successful isolation of VP-15 is a very important step in providing a basic yet suitable material in constructing the dsRNA vaccine to control shrimp diseases in aquaculture.

RESISTENSI UDANG GALAH KETURUNAN PERTAMA TERHADAP INFEKSI Vibrio harveyi Ikhsan Khasani; Alimuddin Alimuddin; Muhammad Zairin Junior; Angela Mariana Lusiastuti; Asep Sopian
Jurnal Riset Akuakultur Vol 10, No 2 (2015): (Juni 2015)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Kematian massal udang galah karena infeksi penyakit merupakan masalah serius pada sistem produksi benih udang galah. Penelitian ini dilakukan untuk meningkatkan resistensi benih udang galah terhadap penyakit vibriosis menggunakan metode seleksi. Larva udang galah GIMacro diinfeksi dengan bakteri patogen Vibrio harveyi untuk mendapatkan populasi benih bertahan hidup, survivor, sebagai pembentuk induk F-0, selanjutnya disebut induk terseleksi. Sub populasi larva dari populasi tersebut tidak diinfeksi dan dipelihara hingga induk sebagai populasi kontrol. Pembentukan populasi F-1 dilakukan dengan mengawinkan antar induk F-0 terseleksi. Infeksi bakteri dilakukan terhadap larva (umur 7-9 hari pascatetas) dengan metode perendaman selama 48 jam, dengan kepadatan awal bakteri V. harveyi sebesar 5 x 105 cfu/mL. Sintasan rata-rata larva dari 24 induk betina adalah sebesar 45,92%-78,50%; dengan koefisien variasi relatif tinggi, sebesar 43%, sehingga seleksi pada karakter tersebut potensial untuk dilakukan. Respons seleksi setelah satu generasi sebesar 10,4% atau peningkatan resistensi sebesar 14,8% dibandingkan kontrol. Sintasan benih F-1 (40,04±11,9%) seleksi pada fase pembenihan standar produksi relatif lebih tinggi dibandingkan benih kontrol (38,04±15,7%). Sintasan benih pada fase pendederan juga demikian, yaitu 78,0±1,7% (F-1) dan 70±4,0% (kontrol). Bobot rata-rata benih udang galah F-1 (23,73±5,40 mg) tidak berbeda nyata dengan benih kontrol (23,40±9,50 mg). Sebagai kesimpulan bahwa peningkatan resistensi udang galah terhadap infeksi penyakit vibriosis dapat dilakukan melalui seleksi berbasis uji tantang.

KLONING cDNA HORMON PERTUMBUHAN DARI IKAN GURAME (Osphronemus gouramy) Estu Nugroho; Alimuddin Alimuddin; Anang Hari Kristanto; Odang Carman; Novi Megawati; Komar Sumantadinata
Jurnal Riset Akuakultur Vol 3, No 2 (2008): (Agustus 2008)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penelitian mengenai kloning cDNA pengkode hormon pertumbuhan ikan gurame telah dilakukan. Tujuan dari penelitian ini adalah untuk memperoleh sekuens DNA komplemen hormon pertumbuhan sebagai langkah awal dalam rangka pengembangan teknologi rekayasa genetik ikan gurame. Empat buah kelenjar hifopisa ikan gurame digunakan sebagai bahan bakunya dan dilakukan proses ekstraksi RNA total dari kelenjar hipofisa, dilanjutkan dengan sintesis cDNA, amplifikasi PCR, purifikasi fragmen DNA dari gel, ligasi produk PCR dengan vektor kloning, transformasi dan inkubasi bakteri, seleksi koloni bakteri putih, isolasi plasmid, dan sekuensing. Hasil sekuensing menunjukkan bahwa panjang produk amplifikasi PCR adalah 843 bp yang menyandikan 204 asam amino residu dan mengandung sekuens-sekuens yang konserf untuk gen hormon pertumbuhan (GH). Analisis homologi menunjukkan kesamaan sekuens hasil isolasi antara 52,4%--97,6% dengan gen GH ikan lainnya, dengan persentase homologi tertinggi adalah dengan ikan sepat. Dengan demikian dapat disimpulkan bahwa sekuens hasil isolasi merupakan sekuens gen GH. Dari hasil analisis sekuens terlihat bahwa gen GH ikan gurame secara evolusi adalah konserf.Research on cDNA cloning encoded the gouramy growth hormone was conducted. The aim of the research was to get complementary DNA, cDNA, sequences of growth hormone as an initial step to develop genetic engineering of gouramy fish. Four pituitary glands of the gouramy were taken and then processed with total RNA extraction, and continued with cDNA synthesis, PCR amplification, DNA fragment purification from the gel, PCR product legation with cloning vector, transformation and incubation of bacteria, white colony bacteria selection, plasmid isolation and sequencing analysis. Sequencing result showed that the amplified PCR product length had 834 bp, encoding 204 amino acid residue and contained conserve sequence for GH (growth hormone) gen. Homolog analysis showed sequence similarity of isolated result between 52.4%—97.6% with other GHs with the highest percentage resulted from the Trichogaster pectoralis. From the sequence analysis showed that GH gen of gouramy at an evolutionary manner was conserve.

DI MORFOMETRIK UDANG JERBUNG (Fenneropenaeus merguiensis de Man) DARI BEBERAPA POPULASI DI PERAIRAN INDONESIA Eni Kusrini; Wartono Hadie; Alimuddin Alimuddin; Komar Sumantadinata; Achmad Sudradjat
Jurnal Riset Akuakultur Vol 4, No 1 (2009): (April 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Udang jerbung, Fenneropenaeus merguiensis merupakan salah satu jenis udang penaeid indegenous yang memiliki prospek untuk dikembangkan sebagai kandidat budidaya tambak. Penelitian ini bertujuan untuk mengetahui keragaman secara morfologi dan jarak genetik udang jerbung dari populasi alam di Selat Sunda, pantai Cilacap, pantai Bengkulu, Selat Lombok, dan pantai Pontianak. Analisis komponen utama (PCA) digunakan untuk mengetahui keragaman morfologi antar populasi alami udang jerbung. Hasil penelitian menunjukkan adanya perbedaan secara morfologi udang jerbung yang diteliti. Populasi Pontianak memiliki keragaman yang paling tinggi dan Bengkulu paling rendah. Dari analisis kluster diperoleh dua kelompok utama yaitu kluster yang pertama terdiri atas Pontianak-Selat Lombok-Bengkulu dan kluster kedua Selat Sunda-Cilacap. Jarak genetik terjauh dimiliki populasi udang jerbung dari Cilacap-Selat Lombok dan terdekat Cilacap-Selat Sunda.Banana prawn, Fenneropenaeus merguiensis, is one of several prospective local crustaceans for shrimp culture. The objective of this study was to reveal the genetic differences among banana prawn populations from Sunda strait, Cilacap coast, Bengkulu coast, Lombok strait, and Pontianak coast. Principle Component Analysis (PCA) was used to determine genetic differences among the five wild populations. Results of the PCA analysis show that there are morphometrically genetic variations among studied banana prawn populations. Banana prawn from Pontianak has the highest variation of morphology. Results of cluster analysis indicate that there are two main clusters where banana prawns from Pontianak, Lombok Strait, and Bengkulu are categorized as the first cluster while Sunda Strait and Cilacap populations are categorized as the second cluster. The highest value of genetic distance is shown by banana prawn populations from Cilacap and Lombok and the lowest value is Cilacap-Sunda Strait.

PEMIJAHAN SEMI-BUATAN SIPUT GONGGONG, Laevistrombus turturella DENGAN INDUKSI KOMBINASI HORMON LHRH-a DAN ANTIDOPAMIN Muzahar - Muzahar; Muhammad Zairin Jr.; Fredinan Yulianda; Muhammad Agus Suprayudi; Alimuddin Alimuddin; Irzal Effendi
Jurnal Riset Akuakultur Vol 14, No 4 (2019): (Desember, 2019)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Gonggong adalah sejenis siput laut yang merupakan makanan laut (seafood) favorit dan ikon Kota Tanjungpinang, ibukota Provinsi Kepulauan Riau (Kepri). Gonggong mengandung protein tinggi, yaitu sekitar 46,65%. Tidak ada laporan tentang produksi budidaya dan upaya konservasi gonggong. Teknologi produksi benih buatan gonggong belum berkembang. Tujuan penelitian ini adalah untuk mengevaluasi pemberian kombinasi hormon LHRH-a dan antidopamin untuk menginduksi proses pemijahan. Evaluasi pemberian hormon LHRH-a dan antidopamin pada pemijahan siput gonggong dilakukan dengan empat dosis: 0,5 ìLgÎ¹ bobot badan lunak (BB) (P1); 0,7 ìLgÎ¹ BB; dan 0,9 ìLgÎ¹ BB (P2); dan tanpa suntikan (TS). Siput gonggong pascasuntikan dipelihara di akuarium selama 14 hari. Hasil penelitian menunjukkan bahwa (1) kombinasi hormon LHRH-a dan antidopamin mampu merangsang pemijahan gonggong. Dosis rendah hormon LHRH-a dan antidopamin (P-1) menghasilkan induk betina yang memijah paling banyak, yaitu 34,48%; lebih tinggi dari P-2 (27,59%), P-3 (20,69%); dan TS (17,24%); (2) jumlah telur yang dikeluarkan oleh induk betina berbeda secara signifikan antar perlakuan (P<0,05). Jumlah telur yang dikeluarkan oleh masing-masing induk berkisar antara 10.874-63.489 butir/ekor dengan rata-rata 39.347 ± 16.667 butir/ekor.The gonggong is a species of sea conch which is a favourite seafood and an icon of Tanjungpinang City, capital of Kepulauan Riau (Kepri) Province. Gonggong contains high protein, about 46.65%. There were no reports on aquaculture production and conservation effort of gonggong. The technology on artificial seed production of gonggong has not yet developed. The aim of this study was to evaluate the administration of LHRH-a hormone and anti-dopamine to induce the spawning process. Evaluation of the administration of LHRH-a hormone and anti-dopamine on the gonggong conch’s spawning was carried out with four doses: 0.5 ìLgÎ¹ soft body weight (BW) (P-1), 0.7 ìLgÎ¹ BW; and 0.9 ìLgÎ¹ BW (P-2); and without injections (TS). The gonggong conchs after injection were reared in aquarium for 14 days. The results showed that (1) a combination of LHRH-a hormone and anti-dopamine was able to stimulate gonggong spawning. The lower dose of LHRH-a hormone and anti-dopamine (P-1) produced the highest number of spawned female broodstock, which was 34.48%, higher than P-2 (27.59%), P-3 (20.69%), and TS (17.24%); (2) the number of eggs released by female broodstock was significantly different among the treatments (P<0.05). The number of eggs released by each female broodstock ranges between 10,874-63,489 grains/ind. with an average of 39,347±16,667 grains/ind.

SUPLEMENTASI PREBIOTIK FRUKTOOLIGOSAKARIDA (FOS) MENINGKATKAN EKSPRESI GEN TERKAIT METABOLISME SERTA PERTUMBUHAN UDANG VANAME, Litopenaeus vannamei Yanti Inneke Nababan; Hasan Nasrullah; Widanarni Widanarni; Munti Yuhana; Alimuddin Alimuddin
Jurnal Riset Akuakultur Vol 16, No 4 (2021): (Desember, 2021)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Fruktooligosakarida (FOS) merupakan salah satu jenis prebiotik yang berpotensi menjadi feed additive dalam budidaya udang vaname. Penelitian ini bertujuan untuk mengevaluasi suplementasi prebiotik FOS melalui pakan terhadap tingkat ekspresi gen terkait metabolisme dan performa pertumbuhan udang vaname. Pakan uji berupa pakan komersil yang disuplementasi prebiotik FOS (Pre) dengan empat dosis berbeda dan masing-masing terdiri atas tiga ulangan yaitu: Pre-0% (kontrol), Pre-0,5%; Pre-1%, dan Pre-2%. Udang dengan bobot rata-rata 1,58 ± 0,21 g dipelihara dalam bak fiber (volume 1 m3) dengan kepadatan 100 ekor per bak. Pemberian pakan dilakukan selama 30 hari dengan dosis 6% dari bobot biomassa udang. Bobot tubuh diukur setiap 10 hari (n=10) dan tingkat ekspresi gen diukur dari hepatopankreas pada akhir pemeliharaan (n=3) dengan metode quantitative-realtime PCR (qPCR). Hasil penelitian menunjukkan bahwa pemberian prebiotik FOS dapat meningkatkan ekspresi gen terkait metabolisme pada udang vaname. Suplementasi prebiotik FOS memberikan bobot rata-rata, pertumbuhan harian, dan tingkat sintasan yang lebih tinggi dibandingkan dengan perlakuan kontrol (p>0,05). Perlakuan Pre-0,5% menunjukkan rata-rata tingkat ekspresi gen tertinggi, namun performa pertumbuhan, dan sintasan tidak berbeda (p>0,05) dengan perlakuan Pre-2%.Fructooligosaccharides (FOS) are natural feed additives with potential application in feed for Pacific white shrimp. This study aimed to evaluate the effects of prebiotic FOS supplementation on the modulation of metabolism-related gene expression and growth performance of Pacific white shrimp. A trial feed consisted of commercial feed supplemented with different doses of FOS: Pre-0% (control), Pre-0.5%, Pre-1%, and Pre-2%; each with triplicate. Pacific white shrimp with an average body weight of 1.58 ± 0.21 g were reared in fiber tanks (d=1.5 m3) with a density of 100 shrimp/tank for each treatment. The shrimp were given the treatment feed five times per day at 6% of the total body mass for 30 days. Shrimp body weight was measured every ten days (n=10). The gene expression level was measured from the hepatopancreas (n=3) by the quantitative-real time PCR (qPCR) method. The results showed that FOS supplementation increased the metabolism-related gene expression levels. FOS supplementation improved the average body weight, average daily growth, and survival higher than control (p<0.05). Pre-0.5% treatment showed the highest average gene expression despite growth performance and survival were not significantly different (p>0.05) compared to Pre-2% treatment. This study concludes that the application of FOS in the feed improves the growth performance of Pacific white shrimp, notably in gained weight, reduced FCR and improved survival rate.

PERFORMA IKAN NILA (Oreochromis niloticus) HASIL SEX REVERSAL, GENETICALLY MALE DAN YY PADA FASE PENDEDERAN PERTAMA Odang Carman; Aulia Saputra; Alimuddin Alimuddin; Maskur Maskur; Dian R Herdianto; Ratu Siti Aliah; Komar Sumantadinata; Tristiana Yuniarti
Jurnal Riset Akuakultur Vol 4, No 1 (2009): (April 2009)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penelitian ini dilakukan untuk mengkaji performa ikan nila hasil sex reversal (SRV), genetically male tilapia (GMT), dan YY pada fase pendederan pertama di akuarium. Benih ikan dipelihara selama 22 hari, dari umur 6 hari hingga 28 hari. Parameter yang diamati meliputi tingkat sintasan, persentase ikan jantan, laju pertumbuhan, dan biomassa. Hasil penelitian menunjukkan bahwa tingkat sintasan tidak berbeda (P>0,05) antar ketiga kelompok ikan dan kontrol (KN), berkisar antara 85,30%--86,20%. Persentase ikan jantan antara SRV (94,5% ± 1,32%) vs. GMT (93,8% ± 1,25%) dan GMT vs. YY (90,2% ± 1,83%) tidak berbeda (P>0,05), sedangkan antara SRV lebih tinggi daripada YY (P<0,05). Persentase ikan jantan pada ketiga kelompok ikan tersebut lebih tinggi (P<0,05) dibandingkan dengan KN (56,9% ± 3,62%). Pertumbuhan ikan YY dan GMT lebih cepat (P<0,05) dibandingkan dengan ikan SRV dan kontrol (KN). Bobot rata-rata ikan YY pada akhir penelitian mencapai 485 mg, ikan GMT 456 mg, ikan SRV 379 mg dan kontrol 342 mg. Produksi biomassa ikan YY, GMT, dan SRV masing-masing sebesar 41,3%; 32,9%; dan 10,3% lebih tinggi dibandingkan dengan KN. Dengan performa yang tinggi dan pertimbangan teknis di lapangan, benih GMT merupakan alternatif yang baik untuk dibudidayakan dalam rangka meningkatkan produksi ikan nila.The experiment was conducted to determine the performance of sex reversed (SRV), genetically male tilapia (GMT), and YY tilapia on first nursery phase in aquarium. Fry were reared for 22 days, from 6 to 28 days-old. Survival rate, percentage of male fish, growth rate and biomass were observed. The result of the study showed that survival rate among fish group and control were similar (P>0.05), ranged from 85.30%-86.20%. Percentage of male fish between SRV (94.5% ± 1.32%) versus GMT (93.8% ± 1.25%) and GMT versus YY (90.2% ± 1.83%) were also similar (P>0.05), while SRV is higher than YY (P<0.05). Percentage of male fish in the three fish groups was higher than that of control (56.9% ± 3.62%). Growth of YY fish and GMT were higher compared to SRV and control fish (KN). The mean weight of YY fish at the end of the experiment reached 476 mg, GMT fish 447 mg, SRV fish 379 mg and control 342 mg. Biomass of YY, GMT and SRV fish were respectively higher by 41.3%, 32.9%, and 10.3% compared to control. With high performance and technical consideration in farm, GMT fish can be a potential alternative to be cultured in fish farm in order to increase aquaculture production of nile tilapia.

Co-Authors . Irmawati . Kurdianto . Suharsono A. Octavera A. Santika Abinawanto ACHIS M. SIREGAR Achmad Sudradjat Ade Sunarma ADITYA BRAHMANDITO Afif Abdurrahman Afpriyaningrum MD AGUS O. SUDRAJAT Agus Oman Sudrajat Agus Priyadi Agus Priyadi Agus Priyadi Ahmad Muzaki AKHMAD TAUFIQ MUKTI Amrullah Amrullah Anang Hari Kristanto Andi Aliah Hidayani Andi Aliah Hidayani Andi Aliah Hidayani Andi Haerul Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Tenriulo Andi Tenriulo Andi Tenriulo Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti ANNA OCTAVERA Anny Hary Ayu Aras Syazili Arief Boediono Arief Boediono Arief Eko Prasetiyo Aris Tri Wahyudi Asep Permana Asep Permana Asep Sopian Asep Sopian ASMI CITRA MALINA A. R. TASSAKKA Aulia Saputra Aulia Saputra Ayi Santika Ayi Santika Ayi Santika Azis Azis Bagus Rahmat Basuki Bambang Iswanto Beginer Subhan Boyun Handoyo Boyun Handoyo Budimawan DARMAWAN SETIA BUDI Darussalam DEDI JUSADI DENNY KHAIRUDI Deny Sapto Chondro Utomo Desy Nurul Astuti Dewi Yuniati Dian Hardiantho Dian Hardiantho Dian Novita Sari Dian R Herdianto Dinamella Wahjuningrum Dinar Tri Soelistiyowati Dinar Tri Soelistyowati Dinar Tri Sulistyowati Dinar Tri Sulistyowati Dody Dharmawan Trijuno Dody Dharmawan Trijuno Dondy Arafat Dwi Hany Yanti Dwi Hany Yanti Dwi Yanti Emma Suryati Emma Suryati Enang Harris Enang Harris Enang Harris Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eni Kusrini Eny Heriyati ENY SUPARYANI Erma Primanita Hayuningtyas Erma Primanita Hayuningtyas Estu Nugroho Evi Tahapari F.H. Pasaribu Fadhila Maharani Putri FADHILLAH RAHMAWATI Fajar Anggraeni Fajar Maulana Fauzan Wahib Alsani Fitriyah Husnul Khotimah Fredinan Yulianda G. Yoshizaki GEMI TRIASTUTIK Gloria Ika Satriani Goro Yoshizaki GORO YOSHIZAKI Goro Yoshizaki Goro Yoshizaki Gunarto Gunarto Gusrina Gusrina H. Arfah Hafidah, Riva Hardiantho D Harton Arfah Hary Krettiawan Haryanti Haryanti HASAN NASRULLAH Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah Hasan Nasrullah HAWIS H. MADDUPPA Herjayanto, Muh. Huria Marnis Huria Marnis I.W. Teguh Wibawan Iis Diatin Ika Safitri Ikhsan Khasani Imron Imron Imron Imron INCE AYU KHAIRANA KADRIAH Indah Mastuti Indah Permata Sari Indra Lesmana Intan Febrianti Irin Iriana Kusmini Irma Andriani Irma Andriani IRMA ANDRIYANI Irmawati IRMAWATI IRMAWATI Irmawati, Budimawan Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal Irvan Faizal IRVAN FAIZAL Irzal Effendi Ita Djuwita Ita Djuwita Jasmadi Jasmadi Jean-Christophe Avarre Joko Santoso Joni Haryadi Jr., Muhammad Zairin Julie Ekasari K. Chaitanya Deepti K. Sumantadinata Kamaruddin Ketut Mahardika Ketut Sugama khairuzzazi Komar Sumantadinata KOMAR SUMANTADINATA Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kukuh Nirmala Kurnia Anggraini Rahmi Lies Emmawati Hadie Luky Adrianto M. SASMITO JATI M. Zairin Junior M.Y. Jamal Maharani, Adinda Maskur Maskur Megawati, Novi MEIMULYA MEIMULYA Melta Rini Fahmi Mia Setiawati Mira Mawardi Mochamad Syaifudin Moh. Tauhid Umar Mohammad Zairin Mohammad Zairin Mubinun Mubinun Muh Alias L. Rajamuddin Muh. Alias L. Rajamuddin MUHAMMAD AGUS SUPRAYUDI Muhammad Firdaus Muhammad Hunaina Fariduddin Ath-thar Muhammad Hunaina Fariduddin Ath-thar Muhammad Husni Amarullah Muhammad Muhammad Muhammad Muttaqin Muhammad Safir Muhammad Yamin Muhammad Zairin Jr Muhammad Zairin Jr Muhammad Zairin Jr. MUHAMMAD ZAIRIN JR. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mulyasari Mulyasari MUNTI YUHANA Muslim Muslim Muzahar N.A. Maswan Nadia Ayuningthias NADIARTI NADIARTI NITA RUKMINASARI Nita Rukminasari Nita Rukminasari Novi Megawati Novi Megawati Nunak Nafiqoh Nunuk Listiyowati Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo NURBETY TARIGAN Nurly Faridah Nurly Faridah O. Carman O.Z. Arifin Odang Carman P. Ratna Kumar Petrus Rani Pong-Masak Priadi Setyawan Pustika Ratnawati Putriku Nadzira Sativa R Rifai R.D. Soejoedono Raden Roro Sri Pudji Sinarni Dewi Raden Roro Sri Pudji Sinarni Dewi Ratu Siti Aliah Ratu Siti Aliah RATU SITI ALIAH Ratu Siti Aliah Rendy Ginanjar Reni Agustina Lubis Ridwan RIDWAN AFFANDI Rommy Suprapto Ruby Vidia Kusumah Rudhy Gustiano RUSTIDJA S. Mariam Samsu Adi Rahman Sari Budi Moria Sembiring Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Setiawati M Shabarullah Siska Aliyas Sandra Siti Aslamyah Siti Fadilah Siti Khodijah Siti Subaidah Siti Zuhriyyah Musthofa Sitti Fakhriyyah SLAMET SUBYAKTO Soelistyowati DT Soko Nuswantoro Sri Murtini Sri Nuryati Sri Nuryati St. Hidayah Triana Suci Antoro Sukenda, Sukenda, SUKMARAHARJA A. TARIGAN Sularto Sularto Sularto Sularto Sutanti Sutanti Syuhada Restu Danupratama T. Takeuchi Tatag Budiardi Toshio Takeuchi Tristiana Yuniarti Uswatun Hasanah Uswatun Hasanah Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti Wartono Hadie Wartono Hadie Wartono Hadie Wartono Hadie Wartono Hadie Wedaraningtyas Nugrahani Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Wiwien Mukti Andriyani Wiwien Mukti Andriyani Y. Hadiroseyani Yanti Inneke Nababan yogi Himawan Yulintine Yulintine Yuni Puji Hastuti Yushinta Fujaya Yushinta Fujaya Zairin Jr M Zakki Zainun

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