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RESISTANCE AGAINST Aeromonas hydrophila INFECTION AND GROWTH OF SECOND GENERATION (F2) AFRICAN CATFISH [Clarias gariepinus] USING SELECTED MOLECULAR MARKERS Alimuddin Alimuddin; Fadhila Maharani Putri; Dinamella Wahjuningrum; Dian Hardiantho; Ade Sunarma; Sri Nuryati
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 2 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1246.846 KB) | DOI: 10.11598/btb.2018.25.2.742

Abstract

Aeromonas hydrophila is a pathogenic bacteria that causes mass mortality in catfish. In previous studies, specific pathogen resistant (SPR), A. hydrophila-resistant African catfish first generation (F1) has been cultivated by marker assisted selection using the major histocompatibility complex (MHC) 1 as a molecular marker. In this study, growth performance, inheritance of the MHC DNA marker in the second generation (F2) of catfish and disease resistance against A. hydrophila infection were observed.  The F2 progenies were produced by crossing F1 fish between themselves. Nursery was performed in 80-L glass aquaria, 4 replications for each cross, at the same initial density, for 2 months of rearing. The results showed that daily growth rate of F2 progenies from the SPR broods was significantly higher than those from broods without the marker.  Results of the PCR analysis showed that average number of F2 progenies from SPR broods carrying the MHC marker was about 91% higher than that of control.  After the fish reached about 12 cm body length, they were challenged by intramuscularly injecting of 0.1 mL A. hydrophila (LD50: 106 CFU ml-1) for 7 days. Results of challenge test showed that survival of F2 offspring from the crosses of SPR broods (77.2%) was about two times higher than those from brood without MHC marker (38.3%).  Differential leukocyte count supported the high resistance of F2 progenies from F1 broods having MHC I marker against A. hydrophila infection. In conclusion, African catfish farming carrying MHC marker potentially have higher productivity and reduces fish lose due to infection by A. hydrophila.
GROWTH PERFORMANCE AND ENZYME ACTIVITIES IN CATFISH [Pangasianodon hypophthalmus] FED WITH WATER HYACINTH-BASED DIET Dewi Yuniati; Nur Bambang Priyo Utomo; Mia Setiawati; Alimuddin Alimuddin
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 2 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.2.840

Abstract

An alternative subtitution of pollard as an imported feed ingredient is a neccesity and one of the potential ingredients is water hyacinth (Eichhornia crassipes). This study was conducted to evaluate growth performance and enzyme activity in catfish (Pangasianodon hypophthalmus). Diet with five different levels of water hyacinth subtitute of pollard (0%, 25%, 50%, 75%, and 100%) were fed to catfish for 60 days. Seventy (70) catfish fry with average initial body weight of 2.45±0.15 g were maintained in 100cm x 80cm x 60cm aquaria. Fish fed at satiation level three times daily at 8 am, 12 pm, and 4 pm. With dietary of 25% water hyacinth, growth performance and protease activity similar to 0% treatment. Feed intake, protein digestibility, feed efficiency, protein efficiency ratio, and protease and amylase enzyme activities decreased (p<0.05) in those fed with more than 25% water hyacinth. Catfish fed 25% water hyacinth showed significantly (p<0.05) higher daily growth rate, feed efficiency and protein digestibility than those with other treatments. Based on the growth performance and enzyme activity, we can conclude that the optimum dietary level of water hyacinth subtitute pollard for fry catfish is 25%.
TRANSPLANTATION OF GIANT GOURAMY (Osphronemus gouramy) TESTICULAR CELLS IN EARLY HATCHING TILAPIA (Oreochromis niloticus) LARVAE Jasmadi Jasmadi; Odang Carman; Alimuddin Alimuddin
Widyariset Vol 2, No 2 (2016): Widyariset
Publisher : Pusbindiklat - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1178.395 KB) | DOI: 10.14203/widyariset.2.2.2016.77-85

Abstract

Cell transplantation technology has been widely developed in mammals, but less implemented in inland aquaculture fish. Giant gouramy (Osphronemus gouramy) is one of important comercial aquaculture commodities that has a relatively long first reproductive cycle compared to the tilapia (Oreochromis niloticus).  Tilapia  has  a  faster  reproductive cycle  and  potential  as  giant gouramy surrogate broodstock. This study was aimed to applicate testicular cell transplantation technology of giant gouramy in tilapia larvae and to evaluate  its  success  rate  trough  donor cell  colonization.  Tilapia  larvae  of 1-2 dph (days post hatching) and 3-4 dph were injected with ±20.000 giant gouramy testicular cells in the peritoneal cavity, and then groomed for two months. Cell colonization of spermatogonium donor was determined by Polymerase Chain Reaction (PCR), using giant gouramy F1-Growth Hormone and R1-Growth Hormone primers. The result of cell dissociation showed that a  bigger  giant gouramy  (827  g)  had  lower  of  spermatogonia  composition (4.45%) than a smaller gouramy (608 g) (14.96%). Donor cell colonization in  1-2 dph  recipient  was  higher  (100%)  than  in  3-4  dph  recipient  (75%). Whereas recipient Survival Rate (SR) was 89.34% for 1-2 dph recipient and 98.96% for 3-4 dph recipient respectively. This study suggests that testicular cell transplantation technology of giant gouramy could be appllied to tilapia (Xenotransplantation)  and  cell  transplantation  has  a better  performance in  1-2  dph  than  in  3-4  dph  recipients.  With such  success,  there  is  a  high potential that the giant gouramy cultivation can be increased through the cell transplantation technology.
IDENTIFICATION OF SNP SPESIFIC MARKER FOR CRUSTACEAN HYPERGLYCEMIC HORMONE GENE: A SOMATIC GROWTH-RELATED IN GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii) Asep Sopian; Alimuddin Alimuddin; Imron Imron; Harry Krettiawan; Fajar Anggraeni; Desy Nurul Astuti
Indonesian Aquaculture Journal Vol 12, No 1 (2017): (June 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (336.842 KB) | DOI: 10.15578/iaj.12.1.2017.7-13

Abstract

High size variation of giant freshwater prawn was found in harvest and resulting in low productivity. Marker assisted selection may be useful to generate broodstock that produces progeny with high growth and homogeneity. This study was conducted to obtain growth related molecular marker in giant freshwater prawn. Genomic DNA was extracted from swimming leg (pleiopods) of 10 giant freshwater prawns fifth Generation for existence of SNP identification, consisted of 5 fast growth (FG) and 5 slow growth (SG).  While for SNP confirmation and resolving power of specific primer studies. The pleiopods sample was taken from six generation of 201 giant freshwater prawns, consisted of 129 fast-growth (FG) with 16.06 ± 2.48 g body weight and 72 slow-growth (SG) with 6.05 ± 0.90 g body weight. Oligonucleotide primers were designed according to Gene Bank database of crustacean hyperglycemic hormone (CHH) gene sequence. The amplified DNA fragment was then sequenced. The results of sequencing showed there was one base different in nucleotides of FG and SG prawns. Six set of primers were designed based on those CH gene sequence. PCR analysis resulted one set of primers which showed a specific amplification product of 280 bp for growth. The result of sequence analysis using the basic local alignment search tools showed that the nucleotide sequence of those PCR products had similarity of 99%-100% with CHH gen of M. rosenbergii. Thus, a candidate of growth related molecular marker have been identified for giant freshwater prawn.
DEVELOPMENTS OF DIGESTIVE TRACT IN LARVAE OF CLIMBING PERCH, Anabas testudineus (Bloch) Yulintine Yulintine; Enang Harris; Dedi Jusadi; Ridwan Affandi; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 5, No 2 (2010): (December 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (349.29 KB) | DOI: 10.15578/iaj.5.2.2010.109-116

Abstract

Climbing perch, Anabas testudineus (Bloch) is a potential species for aquaculture in Kalimantan, Indonesia and belongs to Anabantidae family. The development of its digestive tract was evaluated on larvae reared under culture conditions of 28oC-30oC, from hatching to 30 days after hatching using histological and morphological methods. The larvae were kept in six 100-L tanks. They were fed with rotifers and microalgae from day 2nd after hatching to day 10th; Artemia nauplii from day 7th to day 15th; Artemia meta-nauplii from day 15th to day 20th; and Tubifex worm from day 20th onwards. The development of digestive tract in climbing perch followed the general pattern described for other species. Shortly after hatching, its digestive system was found to be consisted of an undifferentiated straight tube laying dorsally to the yolk sac. At first feeding (day 2nd), both mouth and anus had opened and the yolk sac was partially absorbed. On day 3, the digestive tract was fully differentiated into buccopharynx, esophagus, intestine and rectum. The two pyloric caeca appeared on day 25th after hatching, indicating the transition from larval to juvenile stage and acquisition of an adult type of digestion.
IMMUNE RELATED GENES EXPRESSION ANALYSIS IN KOI FISH AFTER VACCINATED WITH KOI HERPES VIRUS DNA VACCINES Sri Nuryati; Fauzan Wahib Alsani; Hasan Nasrullah; Odang Carman; Yuni Puji Hastuti; Eni Kusrini; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 15, No 1 (2020): (June, 2020)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.15.1.2020.25-32

Abstract

Vaccination is a practical step in preventing diseases caused by koi herpes virus (KHV) in koi fish (Cyprinus carpio haematopterus). We have developed two DNA vaccines for KHV named as GP-25 and GP-11 from two local isolates coded as ORF25 and ORF81, respectively. Although both vaccines have been reported to increase survival rates, the evaluation of koi fish immune responses at the molecular level has not been done post-vaccinations. The aim of this research was to determine the effects of koi herpesvirus DNA vaccine on the immune-modulation of koi fish at mRNA level. This recent research used the best vaccine doses of both vaccines determined from our previous study: 7.5 and 12.5 µg per 100 g fish of GP-11, and 12.5 µg per 100 g fish of GP-25. The immune gene expression was analyzed using the RT-qPCR method from the fish liver at 0, 1, 7, 14, and 28 days post-vaccination (dpv). The results showed that, in the vaccinated fish, the immune genes viz. tumor necrosis factor a (TNFa), interleukin-1b (IL1b), interferon-g (IFNg), Mx1, immunoglobulin Mu chain (IgM), and major histocompatibility complex (MHC) class I and class II were induced to significant extents. The higher dose vaccination using the GP-11 vaccine showed higher immune gene expression than that of the lower dose. Furthermore, the GP-25 vaccine had induced lower immune responses than the GP-11 vaccine when using the same dose of vaccination, but relatively the same when the half-dose of GP-11 vaccine was used. In conclusion, the GP-11 and GP-25 vaccine provided the immune-modulatory effects on the koi fish immune response after vaccination.
GROWTH RESPONSE OF CLOWN LOACH (Chromobotia macracanthus Bleeker 1852) JUVENILES IMMERSED IN WATER CONTAINING RECOMBINANT GROWTH HORMONE Asep Permana; Alimuddin Alimuddin; Wartono Hadie; Agus Priyadi
Indonesian Aquaculture Journal Vol 10, No 2 (2015): (December 2015)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.365 KB) | DOI: 10.15578/iaj.10.2.2015.125-130

Abstract

The main problem in the culture of clown loach (Chromobotia macracanthus) is the slow growth rate, which takes about six months to reach its market size (two inches total body length). Slow growth eventually cause a long production time and increase the production costs. An alternative solution can be proposed in order to enhance the growth is by using recombinant growth hormone. The aim of this study was to determine the immersion dose of recombinant Epinephelus lanceolatus growth hormone (rElGH) which can generate the highest growth in clown loach. Larvae at seven day after hatching were hyperosmotic treated with NaCl 2.0% for one minute, then immersed for one hour in water containing 0.3% NaCl, 0.01% bovine serum albumin (BSA), and different doses of rElGH, namely: 0.12 (treatment A), 1.2 (B), 12 (C), and 120 mg/L (D). As control, fish were immersed in water without rElGH and NaCl (control-1), water containing 0.3% NaCl and 0.01% BSA (control-2), and 0.3% NaCl water (control-3). Each treatment was replicated three times. The results showed that clown loach juveniles in treatment B, C, and D had longer total body length (P<0.05) than control-1, while fish treatment A was the same as controls. Survival and body weight were similar in all treatments and controls (P>0.05). In addition, the percentage of large size juveniles increased approximately 5% in treatment B, almost the same as in the medium size, while the small size were decrease compared to the control-1. Thus, the best immersion dose of rElGH was 1.2 mg/L water.
OVARY DEVELOPMENT, FSH AND LH GENES EXPRESSION OF INDONESIAN LEAFFISH, Pristolepis grootii (Bleeker, 1852), INJECTED WITH LUTEINIZING HORMONE-RELEASING HORMONE ANALOG Muslim Muslim; Agus Oman Sudrajat; Muhammad Zairin Jr.; Muhammad Agus Suprayudi; Arief Boediono; Iis Diatin; Alimuddin Alimuddin
Indonesian Aquaculture Journal Vol 16, No 2 (2021): (December, 2021)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.16.2.2021.69-77

Abstract

Indonesian leaffish, Pristolepis grootii (Bleeker, 1852), is an undomesticated freshwater fish species native to the rivers, flooded swamps, and tributaries of Indonesia. The fish is mainly captured for consumption. In order to prevent its extinction and supply its growing demands, the artificial breeding of the fish should be developed. The purpose of this study was to determine the optimum dose of luteinizing hormone-releasing hormone analog (LHRHa) for stimulating the female P. grootii gonadal development at a dosage of 0, 1, 10, and 50 µg kg-1 of fish. Female fish (20.0 ± 0.6 g) were adapted for 30 days in the rearing environment and then separated into 12 aquariums with six fish per aquarium. Fish were then reared for another 21 days and fed with Tubifex sp. The LHRHa injection was conducted twice on day-7 and 14. Fish bodyweight, gonadosomatic index, gonad histology, blood estradiol-17â, and FSH-â and LH-â gene expression were evaluated at day 0, 7, 14, and 21. The results showed that the injection of the LHRHa hormone stimulated the development of fish gonads and was better achieved with a higher concentration of LHRHa. The best treatment was observed by the administration of 50 µg kg-1 of LHRHa that produced the fastest development among all treatments. This study demonstrated that the LHRHa induction could potentially stimulate the gonadal development of the newly domesticated fish. To our knowledge, this is the first study that reported the success of the induction of female gonad development in the Indonesian leaffish P. grooti.KEYWORDS: 
CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798) Andi Parenrengi; Alimuddin Alimuddin; Andi Tenriulo
Indonesian Aquaculture Journal Vol 12, No 2 (2017): (December, 2017)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.395 KB) | DOI: 10.15578/iaj.12.2.2017.67-75

Abstract

White spot syndrome virus (WSSV) has caused mass mortality on tiger shrimp (Penaeus monodon) culture and adversely affects prawn industry worldwide including Indonesia. It is well known that the protein structure of WSSV plays an important role in the virus infection and morphogenesis process. A viral protein structure called VP-15 is located in the nucleocapsid of virion virus. The protein structure involves in the life cycle of WSSV in host cells. A gene encoding VP-15 could be involved in constructing the RNA interference (RNAi), so it is needed to isolate and characterize for RNAi technology purpose. The study was aimed to isolate and characterize the VP-15 from the infected WSSV tiger shrimp. The characterization of VP-15 was undertaken through assessment of nucleotide sequence, amino acid deduction, alignment nucleotide/protein searches using Genetyx and BLAST program, and dendrogram construction analysis. The results showed that VP-15 was successfully isolated in form of ORFDNA with a fragment size of 243 bp. The phylogenetic tree analysis revealed three clusters corresponding to the time (year) of isolates collection. The VP-15 consisted of 80 amino acids, two start codons (ATG), one stop codon (TAA), and one Kozak context (AAAATGG). Hydrophilic amino acid was the highest composition (44.2%), followed by neutral (31.2%) and hydrophobic (24.6%) amino acid groups. The VP-15 was rich in amino acid of lysine (21.3%), arginine (22.9%) and serine (24.6%). The successful isolation of VP-15 is a very important step in providing a basic yet suitable material in constructing the dsRNA vaccine to control shrimp diseases in aquaculture.
STATUS KESEHATAN IKAN LELE (Clarias gariepinus) YANG MENERIMA PAKAN BERSUPLEMEN KOMBINASI DAUN SIRIH (Piper betler leaf), JAMBU BIJI (Psidium guajava leaf), DAN KIPAHIT (Tithonia diversifolia leaf) Nunak Nafiqoh; Sukenda Sukenda; Muhamad Zairin Junior; Alimuddin Alimuddin; Angela Mariana Lusiastuti; Jean-Christophe Avarre
Jurnal Riset Akuakultur Vol 13, No 4 (2018): (Desember 2018)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.799 KB) | DOI: 10.15578/jra.13.4.2018.357-365

Abstract

Tanaman obat telah banyak digunakan sebagai bahan pencegah dan pengobatan penyakit pada ikan budidaya. Penelitian ini ditujukan untuk mengetahui status kesehatan ikan lele (C. gariepinus) yang menerima pakan dengan suplemen tanaman obat kombinasi dari daun sirih, jambu biji, dan kipahit melalui pengamatan gambaran darah dan histologi ginjal sebagai organ yang memproduksi darah. Kombinasi satu merupakan kombinasi dari ketiga daun tanaman obat masing-masing sebanyak 33%, kombinasi dua juga terdiri dari daun sirih, jambu biji, dan kipahit masing-masing sebanyak 5%:19%:76%, dan kontrol yaitu pakan tanpa penambahan tanaman obat. Gambaran darah dan histologi ginjal diamati pada minggu ketiga setelah pemberian pakan. Hasil pengamatan gambaran darah menunjukkan bahwa terdapat peningkatan jumlah sel darah merah pada ikan yang menerima pakan perlakuan dibandingkan dengan kontrol (0,4 ± 0,14). Namun tidak terdapat perbedaan nyata antara jumlah sel darah merah dari kelompok perlakuan kombinasi satu dan dua (1,5 ± 0,17 dan 1,4 ± 0,1). Jumlah sel darah putih pada kelompok perlakuan juga meningkat dibandingkan dengan kelompok kontrol (10,5 ± 0,46), namun tidak terdapat perbedaan nyata antara kelompok perlakuan kombinasi satu dan dua (15,1 ± 1,19 dan 17,6 ± 1,14). Hasil pengamatan histologi terlihat jaringan hematopoietik organ ginjal dari kelompok yang menerima perlakuan berproliferasi lebih banyak dibandingkan kelompok kontrol. Namun tidak ada pengaruh pada nilai hemoglobin dan persentase leukosit diferensiasi antara kelompok perlakuan dan kontrol. Penambahan daun tanaman obat dalam pakan ikan mampu meningkakan status kesehatan dari ikan lele.Medicinal herbs have been traditionally used as prophylactic and therapeutic supplement to treat diseases in aquaculture. This study was aimed to improve the health quality of catfish (C. gariepinus) through feeding on diets enriched with a combination of betel, guava, and tithonia as medicine by analyzing hematology and histology of kidney as blood producing organ. Diet-one was feed enrich with 33% of each plant. Diet-two was feed enriched with betel, guava, and tithonia at a proportion of 5%,19%, and 76%, respectively. Control diet was fed without the plants’ supplementation. Hematology and histology of fish kidney were observed after fish received three-week feed treatments. The results showed that there was an increase of erythrocyte levels in the treated fish groups fed with diet-one and diet-two compared with the control (0.4 ± 0.14). However, no significant differences of erythrocyte level were observed between fish groups fed with diet -one and die-two (1.5 ± 0.17 and 1.4 ± 0.1). Leucocyte levels also increased in the treated fish group with diet-one and diet-two compared to the control (10.5 ± 0.46). However, there was no significant difference of leucocyte level between the fish group feed with diet-one and diet-two (15.1 ± 1.19 and 17.6 ± 1.14). Histological observations found that there were more hematopoietic tissues in the fish kidney of proliferated treated group than the control group. However, there was no effect on hemoglobin level and leukocyte percentage differentiation between the treatment and control groups. This study concludes that medicinal herbs as enrichment ingredients in fish diet can increase the health quality of fish.
Co-Authors . Irmawati . Kurdianto . Suharsono A. Octavera A. Santika Abdul Halim Talli Abyadul Fitriyah Ade Sunarma Adi Sucipto, Adi Adinda Rizky Tasrianty Afdal Madein Afif Abdurrahman Afpriyaningrum MD Agus Oman Sudrajat Agus Priyadi Ahmad Faisal Ahmad Muzaki Ahmad Ramzi Ghulam Syam Ahmad Zaki Alfiah Mutmainnah Ali Alyaminy, Humaidatul Aminah Zaim Aman Sentosa Panggabean Amin, Muhmmadiyah Andi Aliah Hidayani Andi Haerul Andi Parenrengi Andi Parenrengi Andi Parenrengi Andi Tenriulo Andi, K. Petta Lolo, Arjuni Andi, Maulana K. Angela Mariana Lusiastuti Anggriani, Emi Anhar ANNA OCTAVERA Anny Hary Ayu Apriwanto, Indra Aras Syazili Arief Boediono Arief Eko Prasetiyo Arien Heryansyah Asdar Asep Permana Asep Sopian Asri Ainun Amaliah Aulia Saputra Awi Dassa Ayi Santika Azis Ali Baderiah Baderiah Bahri, Nur Alim Benyamin Timotiwu, Paul Bohari Yusuf Boyun Handoyo Budimawan Chaeroni, Ahmad Chaerunnisa Chotban, Sippah DARMAWAN SETIA BUDI Darussalam Darwin, Khadijah DEDI JUSADI Delfian Masrura Deny Sapto Chondro Utomo Desy Nurul Astuti Devi Wardan Sari Dewi Yuniati Dian Hardiantho Dian Hardiantho Diki Nurbaldatun Islam Dinamella Wahjuningrum Dinar Tri Soelistyowati Djadir Dwi Hany Yanti Eko Pramono Emma Suryati Emma Suryati Enang Harris Enang Harris Endi Permata Eni Kusrini Eni Kusrini Erma Primanita Hayuningtyas F.H. Pasaribu Fachruddin Fachruddin Fadhila Maharani Putri Fajar Anggraeni Fatihah, Anariski Fatimah Akbal Fauzan Wahib Alsani Fauziah Zainuddin Firman Firmansyah Fitriadi Fitriyah Husnul Khotimah Fitry Hasdanita Fredinan Yulianda G. Yoshizaki GEMI TRIASTUTIK Gloria Ika Satriani Goro Yoshizaki GORO YOSHIZAKI Gunawan Gunawan Gustikasari Amin H. Arfah Hadi Nasbey Hamda Hamid Habbe Hamzah Hanipa Hardiantho D Harmayani, Ria Harton Arfah Hary Krettiawan Haryanti Haryanti Hasan Nasrullah Hassan, Muhammad Nasiru Herjayanto, Muh. Hisyam Ihsan Ibnu Izzah Idrus Sahar Muhammad Iis Diatin Ika Kartika Ikhwanul Arifan ILHAM Ilham Minggi Imron Imron Indah Mastuti Irma Andriani IRMA ANDRIYANI Irmawati, Budimawan Irvan Faizal Irvan Faizal IRVAN FAIZAL Irwan Irzal Effendi Ita Djuwita Jasmadi Jasmadi Jean-Christophe Avarre Joko Santoso Jubaedah Julie Ekasari Jumrah123 K. Chaitanya Deepti K. Sumantadinata Kadriani, Nur Annisa Kamaruddin Kartini, Selvira Febriani Katarina Manik, Tumiar Ketut Mahardika Ketut Sugama Khaerullah khairuzzazi Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata KOMAR SUMANTADINATA Leman, Swanggi Lianasari, Ika Yekti Lidyasari Liza Luky Adrianto M. SASMITO JATI M. Zairin Junior M.Y. Jamal Madein, Afdal Maharani, Adinda Marwah, Andi Marwah Mashud MEDIATY Megawati, Novi Mia Setiawati Miftahul Jannah Mira Mawardi MMSI Irfan ,S. Kom Mochamad Syaifudin Muh Alias L. Rajamuddin Muh. Akmal, Andi Muh. Alias L. Rajamuddin MUHAMMAD AGUS SUPRAYUDI Muhammad Ammar Naufal Muhammad Awal Ramadhan Muhammad Firdaus Muhammad Ikhsan Muhammad Jufri Muhammad Muttaqin Muhammad Sabir Muhammad Safir Muhammad Wawan Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammadiyah Amin MUNTI YUHANA Muslim Muslim Mustika Sari, Mustika Musyfikah Ilyas Muzahar N.A. Maswan Nadhirah Nagu Nagu, Nadhirah Nasrullah Nenima Halawa Nining Asniar Ridzal Noerlitasari Nugraha, Adrianto Fahrizal Nunak Nafiqoh Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur, Rezki Amaliah Nurdin Arsyad Nurfadilla Nurhalija Nurhayati Nurhidayat, Muhammad Nurlaelah Nurlaelah Syarifuddin Nurly Faridah Nurly Faridah Nurul Fadhilah Nurwati Djam’an O. Carman O.Z. Arifin Odang Carman Oman Unju Subandi P. Ratna Kumar Permata Sari, Anggun Petrus Rani Pong-Masak Priatama, Rizki Purwaningsih, Yuli Pustika Ratnawati Putri, Angelina Atika R Rifai R.D. Soejoedono Rahmi, Kurnia Anggraini Ratu Siti Aliah Ratu Siti Aliah RATU SITI ALIAH Reni Agustina Lubis Ridwan RIDWAN AFFANDI Risfiyanty, Dwi Kartika Riswandi Rizky Ayu Fatimah Ridwan Rodliyah Rohayu, Rina Rosidah Rusli RUSTIDJA S. Mariam Samsu Adi Rahman Sani, NurHaslina Sanusi Saputra, Ade Maulana Sari , Nur Indah Sari Budi Moria Sembiring Sarmila Sekar Sulistyaning Hadiwibowo Setiawati M Shabarullah Shalsyabila , Zabina Shandi Irawan Sholeh Ridwan Siti Aslamyah Siti Fadilah Sitti Fakhriyyah SLAMET SUBYAKTO Soelistyowati DT Soerja Koesnarpadi Soko Nuswantoro Sri Khayati sri murtini . Sri Nuryati Sri Nuryati Srihartini, Yusi St. Fatmawati. L St. Hidayah Triana St. Mukarramah Sukenda . Sukenda Sukenda Sukmawati Ali Sumardin Raupu Sundari Sutamrin Syafrianto, Syafrianto Syuhada Restu Danupratama T. Takeuchi Tahrim, Tasdin Talib, Dr. Ahmad Tasdin Tahrim Tedy Murtedjo Tri Setyo Guntoro Usman Mulbar Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti Wa Ode NurRadhiah Ridjalu Wahidah Sanusi Wartono Hadie Wicaksono, Aryo Wenang WIDANARNI WIDANARNI Y. Hadiroseyani Yulintine Yulintine Yuni Puji Hastuti Yuningsih, Andri Yusra, Andi Zairin Jr M Zakki Zainun