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All Journal Jurnal Akuakultur Indonesia ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) BERITA BIOLOGI Omni-Akuatika LIMNOTEK - Perairan Darat Tropis di Indonesia Jurnal Perikanan Universitas Gadjah Mada Jurnal Intek Akuakultur Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Abdi Insani Jurnal Pusat Inovasi Masyarakat Journal of Fisheries & Marine

Agus Oman Sudrajat

Department Of Aquaculture, Faculty Of Fishery And Marine Sciences, Bogor Agricultural University, Darmaga, Bogor 16680, Indonesia

Author-ID : 2472806

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Social Sciences Veterinary Other

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CANNIBALISM PERFORMANCE OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) FED RATION SUPPLEMENTED WITH DIFFERENT DOSES OF 17α-METHYLTESTOSTERONE Heltonika, Benny; Sudrajat, Agus Oman; Junior, Muhammad Zairin; Widanarni, Widanarni; Suprayudi, Muhammad Agus; Manalu, Wasmen; Hadiroseyani, Yani
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.27-35

One of the obstacles in Asian redtail catfish hatchery is the high cannibalism incidence. Cannibalism is associated with aggressive behavior caused by hormonal metabolism especially of testosterone. The purpose of this study was to evaluate the effect of testosterone administration on the cannibalism incidence in the Asian redtail catfish juveniles. The experiment was conducted by rearing fish with initial length of 4.09±0.19 cm in 16 of 20 L aquariums with a density of 2 fish L-1. The experiment was designed with a completely randomized design with 4 treatments which were different level of 17α-methyltestosterone supplementation in feed, i.e., 0 mg kg-1 feed (A) as control, 7.5 mg kg-1 feed (B), 15 mg kg-1 feed (C), and 30 mg kg-1 feed (D). Each treatment has 4 replications. Fish fed experimental diet (40% protein) 4 times a day to satiation for 30 days. The parameters observed were type and index of cannibalism, aggressive behavior, survival rate, normal mortality, growth performance, hormones concentrations (estradiol, testosterone, and cortisol), and water quality. The results showed that cannibalism type II (the fish eaten completely or missing) and cannibalism index increased with the increasing doses of testosterone administration in the feed with the highest cannibalism incidence was 40.63%. The highest survival rate was found in treatment B (73.75±2.50%) and was not significantly different from the control treatment (69.38±2.39%). No differences in testosterone concentration and in the growth performance among the treatments. However, there was a trend of decrease in the estradiol concentration of Asian redtail catfish juveniles fed ration supplemented with the increasing doses of 17α-methyltestosterone. Based on the results obtained in this research, estradiol changed in the body's plasma, it appears that there was a role for plasma estradiol concentration in controlling cannibalism of Asian redtail catfish juveniles. It concluded that the testosterone administration affected the cannibalism incidence in the Asian redtail Catfish.

EVALUASI MASKULINISASI PLATI PEDANG, Xiphophorus helleri MENGGUNAKAN KOMBINASI EKSTRAK BUAH PINUS, Pinus merkusii DENGAN AROMATASE INHIBITOR Akbar, Muhamad Saepul; Sudrajat, Agus Oman; Arfah, Harton; Soelistyowati, Dinar Tri
Media Akuakultur Vol 19, No 1 (2024): Juni 2024
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.19.1.2024.9-15

Hormon 17α-methyltestostrone sudah banyak digunakan untuk maskulinisasi pada kegiatan budidaya namun, bahan tersebut bersifat karsinogenik sehingga diperlukan alternatif pengganti bahan tersebut. Penelitian ini bertujuan mengevaluasi efek kombinasi ekstrak buah pinus (Pinus merkusii) dan aromatase inhibitor pada ikan plati pedang. Sebanyak 5 perlakuan menggunakan bahan berbeda, yaitu 17α-methyltestostrone, ekstrak buah pinus, aromatase inhibitor, kombinasi ekstrak buah pinus dan aromatase inhibitor, serta kontrol dengan masing-masing 3 ulangan. Perendaman dilakukan selama 6 jam, kemudian ikan dipelihara selama 60 hari. Rasio induk jantan dan betina yang digunakan untuk pemijahan ikan pada penelitian ini adalah 1:3. Penelitian ini berfokus pada tiga parameter, yakni Tingkat Kelangsungan Hidup (TKH), Nisbah Kelamin Jantan (NKJ) dan jumlah ikan jantan yang dihasilkan pada tiap perlakuan. Hasil penelitian menunjukkan ikan yang direndam pada bahan berbeda menghasilkan nilai nisbah kelamin jantan yang lebih tinggi. Dosis terbaik pada penelitian ini adalah perendaman dengan ekstrak buah pinus dengan dosis 0,02 mL L-1.The hormone 17α-methyltestostrone has been widely used for masculinization in aquaculture, however, this material is carcinogenic and it is required to find its alternative substitutions. The aim of this research to evaluate the effects of combination of pine cone (Pinus merkusii) extract and aromatase inhibitor on swordtail fish. A total of 5 treatments using different materials, namely: 17-α methyltestosterone, pine cone extract, aromatase inhibitor, pine cone extract mixed with aromatase inhibitor, and control with 3 replications. Immersion was conducted for 6 hour and the the fish were kept for 60 days. The ratio of male to female parents used for fish spawning was 1:3. The study focused on three parameters, i.e. survival rate, male fish percentage, and number male fish produced. The results revealed fish immersed with different materials exhibited higher male fish percentage compared to the control group (not immersed). The best treatment in this research was immersion using pine cone extract with dosage of 0.02 mL L-1.

Characterization of probiotic Bacillus sp. NP5 metabolites and their effect on female catfish broodstock re-maturation Enzeline, Valensia; Widanarni, Widanarni; Sudrajat, Agus Oman; Alimudin, Alimudin
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.58-70

Probiotics are believed to contain active components that influence fish reproduction. This study aimed to evaluate the metabolite compounds present in the probiotic Bacillus sp. NP5 and their effect on female catfish broodstock re-maturation. The first step involved analyzing the metabolite content of the probiotic using liquid chromatography with tandem mass spectrometry (LCMS/MS). Subsequently, feed supplementation with the probiotic was implemented at concentrations of 106 CFU/g (P6), 108 CFU/g (P8), and control (C). Parameters observed included the metabolite content of Bacillus sp. NP5, gonadosomatic index (GSI), hepatosomatic index (HSI), gonad histology, gonad maturity rate, fecundity, number of matured eggs, distribution of egg diameter, fertilization rate (FR), hatching rate (HR), larval survival rate (SR), re-maturation period, and percentage of matured broodstock. The results showed that metabolites such as resveratrol (22.45%) and indoline (7.11%) are likely to influence the fish reproduction directly. Other metabolites, such as oxyresveratrol (22.95%) and vestitol (4.14%), are also thought to play an indirect role in reproduction. The supplementation demonstrated the best reproductive performance at 108 CFU/g treatment, with an increased GSI (14.74%), HSI (1.65%), gonad maturity rate, percentage of mature eggs (86.00%), re-maturation period (4 weeks), percentage of matured broodstock (100%), fecundity (243,605 eggs/broodstock), FR (98.74%), HR (80.62%), and SR (87.31%). The application of probiotic Bacillus sp. NP5 can accelerate re-maturation and enhance reproductive performance of female catfish broodstock. Probiotics contain metabolites that positively affect reproduction and can serve as an alternative to improve reproductive performance in female catfish broodstock. Keywords: Bacillus sp. NP5, female catfish, metabolites, probiotics, re-maturation ABSTRAK Probiotik diyakini mengandung komponen aktif yang berpengaruh terhadap kinerja reproduksi ikan. Tujuan penelitian ini adalah mengevaluasi senyawa metabolit yang terkandung dalam bakteri probiotik Bacillus sp. NP5 dan pengaruhnya terhadap rematurasi induk ikan lele betina. Tahapan pertama adalah analisis kandungan metabolit probiotik Bacillus sp. NP5. dengan metode liquid chromatography with tandem mass spectrometry LCMS/MS. Selanjutnya dilakukan suplementasi pakan dengan perlakuan probiotik pada konsentrasi 106 CFU/g (P6), 108 CFU/g (P8), dan kontrol (K). Parameter yang diamati meliputi kandungan metabolit Bacillus sp. NP5, indeks gonadosomatik (IGS), indeks hepatosomatik (IHS), histologi gonad, tingkat kematangan gonad (TKG), fekunditas, jumlah telur matang, sebaran frekuensi diameter telur, derajat pembuahan (FR), derajat penetasan (HR), sintasan larva (SR), periode rematurasi, dan persentase induk matang gonad. Hasil penelitian memperlihatkan kandungan metabolit seperti resveratrol (22,45%) dan indoline (7,11%) diduga secara langsung berpengaruh terhadap reproduksi. Metabolit lain yang ditemukan seperti oxyresveratrol (22,95%) dan vestitol (4,14%) juga diduga berperan secara tidak langsung. Hasil suplementasi menunjukkan bahwa performa reproduksi terbaik didapat pada perlakuan 108 CFU/g pakan dengan peningkatan nilai IGS (14,74%), IHS (1,65%), TKG, persentase telur matang (86,00%), periode rematurasi (4 minggu), proporsi induk matang gonad (100%), fekunditas (243,605 butir telur/kg bobot induk), FR (98,74%), HR (80,62%), dan SR larva (87,31%). Pemberian probiotik Bacillus sp. NP5 dapat mempercepat rematurasi dan meningkatkan kinerja reproduksi induk ikan lele betina. Probiotik mengandung bahan-bahan metabolit yang berpengaruh positif terhadap reproduksi dan dapat menjadi alternatif dalam meningkatkan performa reproduksi pada induk ikan lele betina. Kata kunci: Bacillus sp. NP5, ikan lele betina, metabolit, probiotik, rematurasi

Feeding with Artificial Feed on Sand Goby, Oxyeleotris marmorata (Blkr.), Fry Sudrajat, Agus Oman; Effendi, Irzal
Jurnal Akuakultur Indonesia Vol. 1 No. 3 (2002): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACTThe influence of feed shape and protein resource of artificial feed on the growth and survival rate of sand goby, Oxyeleotris marmorata (Blkr.), fry were evaluated in this experiment. This experiment purposed to determine growth rate, survival rate, feeding efficiency, protein retention, lypid retention and look for the suitable artificial feed for sand goby. This experiment was carried out at the Laboratory of Aquaculture System and Technology, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University in Bogor. Factorial design was used in this experiment with two factors: feed shape and protein resource. There were two kinds of feed shape: dry pellet and moist pellet. Protein resource consisted: fish, squid, and shrimp. The sand goby juveniles were kept in aquarium 60x30x40 cm was filled 40 litre of aerated. The fish were fed of 7% (dry weight based) of body weight in three time of feeding (10:00, 14:00, 18:00 WIB), 30, 30, 40% total feed/day respectively. The amount of feed was adjusted every 7 days with sampling. The juveniles feeding with shrimp-moist showed better survival rate, growth rate, feeding efficiency, protein retention, lypid retention than those fed shrimp-dry, squid-dry, squid-moist, fish-dry, and fish-moist. Dry pellet and moist pellet can be used for sand goby feeding. The artificial feed for sand goby juvenile suggested contain attractant and had highly water stability. Shrimp and squid can be used as main protein resources in artificial feed for sand goby. Key Word : Feeding, artificial feed, sand goby, Oxyeleotris marmorata, fry. ABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh bentuk pakan dan sumber protein dari pakan buatan terhadap pertumbuhan dan kelangsungan hidup ikan betutu, Oxyeleotris marmorata (Blkr.). Rancangan faktorial dengan 2 faktor, bentuk pakan (pelet basah dan pelet kering) dan sumber protein (ikan, cumi dan udang) digunakan dalam penelitian ini. Ikan diberi pakan 7% bobot badan basah yang diberikan dalam 3 kali per hari. Ikan yang diberi pakan dengan kombinasi bentuk pelet basah dan sumber protein udang (pelet basah-udang) menunjukkan kelangsungan hidup, pertumbuhan, efisiensi pemberian pakan dan retensi protein terbaik dari perlakuan lainnya. Udang dan cumi dapat digunakan sebagai sumber protein utama dalam pakan buatan untuk ikan betutu. Pakan buatan untuk benih ikan betutu disarankan mengandung atraktan dan memiliki stabilitas dalam air yang tinggi.Kata kunci : Pemberian pakan, pakan buatan, ikan betutu, Oxyeleotris marmorata, benih.

Survival Rate and Growth of Fighting Fish Larvae (Betta splendens Regan) Fed on Various Live Foods Budiardi, T.; Nursyams, T.; Sudrajat, Agus Oman
Jurnal Akuakultur Indonesia Vol. 4 No. 1 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Larval of fighting fish (Betta splendens Regan) requires precise live foods for its growth and survival. In this experiment, fish larvae were fed on either Paramecium + Artemia, Paramecium + Artemia + Tubifex, Paramecium + Moina, or Paramecium + Moina + Tubifex. The fish were fed Paramecium from day-2 till day-7 after hatching. There after, the live food was changed according to the treatments till day-28. Results showed that fish fed on Paramecium + Artemia significantly had the highest total length (12.63 mm) than other treatments (11.86 mm). On the other hand, survival rate of fish had no significant affected by the treatments. Keywords: fighting fish, Betta splendens, Paramecium, Moina, Artemia, Tubifex, larvae ABSTRAK Larva ikan betta (Betta splendens Regan) membutuhkan jenis pakan alami yang tepat bagi kelangsungan hidup dan pertumbuhannya. Pada penelitian ini, larva ikan diberi pakan berupa Paramecium + Artemia, Paramecium + Artemia + Tubifex, Paramecium + Moina, atau Paramecium + Moina + Tubifex. Ikan diberi pakan pakan berupa Paramecium dari hari ke-2 hingga hari ke-7. Setelah itu, pemberian pakan alami diubah berdasarkan masing-masing perlakuan hingga hari ke-28. Hasil penelitian menunjukkan bahwa ikan yang diberi pakan Paramecium + Artemia memiliki tubuh secara signifikan lebih panjang (12,63 mm) dibandingkan perlakuan lainnya (11,86 mm). Sementara itu, kelangsungan hidup tidak dipengatuhi oleh perlakuan. Kata kunci: ikan betta, Betta splendens, Paramecium, Moina, Artemia, Tubifex, larva

Gonad Maturation of Clown Loach (Botia macracanthus) in Pond Effendi, Irzal; Prasetya, T.; Sudrajat, Agus Oman; Suhenda, N.; Sumawidjaja, K.
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The objective of this research is to know the gonad maturation of clown loach {Botia macracanthus) reared in pond. Two groups contain 4 female broodstock; I) carried and II) non-carried egg broodstock were reared in two separated hapas which placed in pond. Each hapa was also stocked nine males. The fish were fed pellet (32,33% protein) 10% biomass, daily in three times. After 20 days, the broodstocks were implanted by LHRH-a 100 u.g/kg of body weight. In the group I, diameter of egg in gonad were developed from 1,028 mm at the beginning of implantation to 1,071 and 1,106 mm at day of 15 and 30 after implantation respectively. In the group II, only one female has developed her gonad successfully. The egg was developed to 0,937 and 1,026 mm after 15 and 30 day implantation respectively. Key words : Gonad maturation, clown loach, Botia macracanthus, pond ABSTRAK Penelitian ini bertujuan untuk mengetahui pematangan gonad induk ikan botia (Botia macracanthus) yang dipelihara di kolam. Dua kelompok induk betina; I) sudah mengandung telur dan II) belum mengandung telur masing-masing sebanyak 4 ekor dipelihara masing-masing dalam 2 hapa. Ke dalam setiap hapa juga ditempatkan induk jantan sebanyak 9 ekor. Induk diberi pakan berupa pelet (protein, 32,33%) sebanyak 10% dari bobot biomasa per hari, 3 kali sehari, dan diberi LHRH-a dengan dosis 100 ng/kg bobot tubuh secara implantasi pada hari ke 20 pemeliharaan. Induk dalam kelompok pertama telurnya berkembang dari rata-rata 1,028 pada saat pemberian LHRH-a menjadi rata-rata 1,071 dan 1,106 mm masing-masing pada hari ke 15 dan 30 setelah pemberian. Pada kelompok kedua hanya satu ekor induk yang berkembang gonadnya setelah diberi LHRH-a. telur induk ikan tersebut berkembang dari 0,937 menjadi 1,026 mm masing pada hari ke 15 dan 30 setelah implantasi. Kata kunci : Pematangan gonad, ikan botia, Botia macracanthus, kolam

Study of gonadotropin (GtH) stimulating hormone on gonad maturation of climbing perch Anabas testudineus Bloch Suriansyah, .; Junior, M. Zairin; Sudrajat, Agus Oman
Jurnal Akuakultur Indonesia Vol. 9 No. 1 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Gonadotropin can quicken the process of 17α-hidroxyprogesterone hormone synthesis becoming 17α, 20β-di hidroxyprogesterone as the maturation inducing steroids (MIS) and quicken the process of egg nucleus integration to germinal vesicle breakdown (GVBD) position. This research aimed to know the efficacy of gonadotropin hormone in the form of ovaprim on gonad maturation of climbing perch (Anabas testudineus Bloch). Stimulation of GtH with a dose of 0.5 ml/kg of fish body weight could improve the fish gonado somato index (GSI) to 2.72 %, improve the the final egg diameter to 0.70 mm (71.50 %), and shorten ovulation time which down to 4.30 hours. Key words: Gonadotropin hormone, gonad maturation, Anabas testudineus ABSTRAK Hormon gonadotropin dapat mempercepat proses sintesa hormon 17α-hidroksiprogesteron menjadi 17α, 20β-dihidroksiprogesteron yang berfungsi sebagai steroid yang merangsang pematangan gonad dan mempercepat proses integrasi inti sel telur menuju posisi germinal vesicle breakdowan (GVBD). Penelitian ini bertujuan untuk mengetahui efektivitas hormon gonadotropin yang terdapat dalam ovaprim terhadap pematangan gonad ikan betook Anabas testudineus Bloch. Pemberian hormon GtH dalam bentuk ovaprim dengan dosis 0,5 ml/kg bobot ikan dapat memperbaiki perkembangan gonad yang ditunjukkan dengan peningkatan nilai gonado somatik indeks (GSI) sebanyak 2,72%, peningkatan diameter telur menjadi 0,77 mm (71,50%) dan mempercepat waktu ovulasi menjadi 4,3 jam. Kata-kata kunci: Hormon gonadotropin, pematangan gonad, ikan betok

Effect of Stocking Density on Growth and Survival Rate of Giant Gouramy (Osphrenemus gouramy Lac.) Seed Sarah, S.; Widanarni, .; Sudrajat, Agus Oman
Jurnal Akuakultur Indonesia Vol. 8 No. 2 (2009): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This experiment was conducted to study the stocking density effect on growth and survival rate of giant gouramy seed in the aquarium. Fish were stocked at the density of 2.5; 5.0; 7.5 and 10 individual/l with average initial weight and length were 0.013 g and 5.56 mm, respectively. Fish were fed with silkworm (Tubifex sp.) ad libitum in the morning and evening for 22 days of experiment. Aquarium was siphoned and water was changed everyday for maintaining good water quality. Result showed that survival rate remain high in all treatment (93.5 - 95.5%). Increasing stocking density from 2.5 to 10 individual/I did not affect survival rate. On the other hand growth rate and feed efficiency decreased, while fish yield increased. Stocking density of 2,5 individual/l gave the highest daily growth rate, individual growth rate, growth in length and feed efficiency of 12.94%, 0.0081 g/day, 16.84 mm and 12.51%; respectively. The highest value for fish yield (0,0360 g/l/day) was obtained from stocking density of 10 individual/l Key words: stocking density, seed, survival rate, growth rate, giant gouramy Osphrenemus goramy ABSTRAK Keterbatasan pengadaan benih ikan gurame (Osphronemus gourame Lac.) disebabkan oleh tidak seimbangnya jumlah benih yang tersedia dengan kebutuhan usaha pembesaran. Teknik pembenihan yang digunakan sampai saat ini relatif sederhana dengan cara tradisional. Pemeliharaan gurame secara terkontrol di akuarium dapat menjawab tantangan dalam teknologi pembenihan gurame sekaligus sebagai sarana pola budidaya secara bertahap yang sedang berkembang saat ini. Produksi yang tinggi akan dicapai dengan pemeliharaan pada kepadatan yang tinggi. Pada keadaan lingkungan yang baik dan pakan yang mencukupi, peningkatan kepadatan akan disertai dengan peningkatan hasil. Jumlah ikan yang ditebar pada penelitian ini disesuaikan berdasarkan perlakuan yaitu 2,5; 5,0; 7,5 dan 10 ekor/l. Pengamatan terhadap ikan dilakukan sampai hari ke-22. Hasil pengamatan menunjukkan bahwa peningkatan kepadatan 2,5 - 10 ekor/l pada pemeliharaan benih gurame di akuarium mempengaruhi pertumbuhan, hasil dan efisiensi pakan, namun tidak berpengaruh terhadap kelangsungan hidup. Dengan meningkatnya padat penebaran, laju pertumbuhan dan efisiensi pakan semakin menurun, sedangkan hasil (yield) semakin meningkat. Padat penebaran 2,5 ekor/l menghasilkan laju pertumbuhan harian, laju pertumbuhan individu, pertumbuhan panjang mutlak dan efisiensi pakan tertinggi, masing-masing mencapai 12,94 %, 0,0081 g/hari, 16,84 mm dan 12,51 %. Sedangkan nilai tertinggi untuk hasil (yield) sebesar 0,0360 g/l/hari diperoleh pada padat penebaran 10,0 ekor/l. Kata kunci : padat tebar, pembenihan, gurame dan Osphronemus gouramy

Production and bioactivity test of recombinant protein common carp growth hormone Utomo, Deny Sapto Chondro; Alimuddin, .; Sudrajat, Agus Oman; Faizal, Irvan
Jurnal Akuakultur Indonesia Vol. 10 No. 1 (2011): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

This study aimed to produce recombinant growth hormone (rGH) protein of common carp (Cyprinus carpio) and evaluate its bioactivity. DNA fragment encoding mature GH protein of common carp (mCcGH) was amplified by polymerase chain reaction (PCR) method and PCR products were then ligated into pCold I to generate pCold I-mCcGH protein expression vector. Escherichia coli BL21 (DE3) harboring pCold I-mCcGH was cultured in the 2xYT medium at 15 °C for 24 hours and protein production was induced by isopropyl-beta-thio galactopyranoside (IPTG). The inclusion bodies containing rGH protein from E. coli transformants were isolated by sonication method and analyzed by SDS-PAGE. The result showed that rGH with molecular weight of about 25 kDa was obtained. Common carp juveniles with average body weight of 5.2±0.4 g were intramuscularly injected once a week for 4 weeks with rGH protein solution from 1 μg bacterial cells per gram fish body weight. The result showed that juveniles fish injected with rGH grew 106.56% higher than control. This result indicated that rGH produced in E. coli BL21 possessed biological activity and it may be useful to improve growth of aquaculture species.Key words: growth hormone, recombinant protein, common carp ABSTRAKPenelitian ini bertujuan menghasilkan protein rekombinan hormon pertumbuhan (growth hormone, GH) dari ikan mas (Cyprinus carpio) dan menguji bioaktivitasnya. Fragmen DNA penyandi protein matang (mature) GH ikan mas (mCcGH) diamplifikasi dengan menggunakan metode PCR dan hasilnya kemudian diligasi ke dalam pCold-I untuk menghasilkan konstruksi vektor ekspresi pCold-I-mCcGH. Plasmid pCold-I-mCcGH ditransformasi ke bakteri Escherichia coli BL21 (DE3), dikultur dalam media 2xYT cair pada suhu 15°C selama 24 jam dan produksi protein diinduksi dengan menggunakan isopropyl-beta-thio galactopyranoside (IPTG). Badan inklusi yang mengandung protein rekombinan GH (rGH) dari bakteri E. coli transforman diisolasi menggunakan metode sonikasi dan dianalisis dengan menggunakan SDS-PAGE. Hasil penelitian menunjukkan bahwa rGH dengan bobot molekul sekitar 25 kDa berhasil diproduksi. Benih ikan mas dengan bobot rata-rata 5,15±0,4 g diinjeksi secara intramuskular satu kali per minggu selama 4 minggu dengan larutan rGH hasil ekstraksi dari 1 µg pelet bakteri/g bobot ikan. Benih yang disuntik dengan rGH tumbuh sekitar 100% lebih cepat bila dibandingkan dengan kontrol yang tidak diinjeksi rGH. Hasil ini mengindikasikan bahwa rGH yang diproduksi dalam bakteri E. coli memiliki bioaktivitas dan dapat bermanfaat untuk memacu pertumbuhan spesies ikan-ikan budidaya.Kata kunci: hormon pertumbuhan, protein rekombinan, ikan mas

Masculinization on tilapia (Oreochromis sp.) by natural hormone steroid agent from bee resin through artificial diet Soelistyowati, Dinar Tri; Sudrajat, Agus Oman; Arfah, Harton
Jurnal Akuakultur Indonesia Vol. 9 No. 2 (2010): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Masculinization on tilapia ( Oreochromis sp.) has been done with approach of natural hormone steroid agent from bee resin through artificial diet before sexual differentiation. This research aim to increase male sex ratio and production performance. Treatment of bee resin dose is 0.6; 1.2; 1.8; 2.4; 3.0 feed ml/kg compared to Control (0 ml/kg diet) were given during 28 days at larva age 7 day in aquarium, then transferred to the farm ground until age 12 weeks. Average of masculine phenotype percentage increased significantly (P

Co-Authors , Alimuddin . Suriansyah Ade Yulita Hesti Lukas Afif Abdurrahman Agoes Mardiono Jacoeb Akbar, Muhamad Saepul Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimudin Alimudin, Alimudin Andin Dwi Fitri Rahayu Andri Yanto Andriyanto Andriyanto Andriyanto Andriyanto Antharest Sugati, Antharest Aprelia Martina Tomasoa, Aprelia Martina Apriana Vinasyiam Arief Boediono Arlita, Kriswidya Armen Nainggolan Asda Laining Awaludin Bambang Gunadi Bambang Gunadi Bambang Gunadi Bastiar Bastiar Benny Heltonika Citra Fibriana Cut Dara Dewi Damiana Rita Ekastuti Daniel Djokosetiyanto Deni Radona, Deni Deny Sapto Chondro Utomo Didik Ariyanto Didik Ariyanto Dinamella Wahjuningrum Dinar Tri Soelistyowati Dony Prasetyo Dwi Mulyasih Dythia Asma Nadia Edison, Thomas Eka Kusuma Eko Harianto, Eko Eko Rini farastuti Enang Harris Eni Kusrini, Eni Eny Heriyati Enzeline, Valensia Epro Barades Erma Primanita Hayuningtyas Fahmi Akbar, Fahmi Fauzan Fajari Gustiano, Rudhy Hadra Fi Ahlina Hafidah, Riva Hafif Syahputra Halawa, Nenima Hanggara, Yudha Harton Arfah Haryanti Haryanti Haya Audina Azizia Helena Sahusilawane Herawati Rasid Hesti Wahyuningsih Hirmawan Tirta Yudha Iis Diatin Imron Indra Lesmana Irvan Faizal Irvan Faizal Irzal Effendi Jonathan Felix Raynar Joni Haryadi, Joni Jr, Muhammad Zairin Jufri, Fatahillah Maulana K. Sumawidjaja Kapelle, Imanuel B. D. Klea Chandra Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Ligaya I. T. A. Tumbelaka Luki Abdullah M. Zairin Junior Mahdaliana, Mahdaliana Mala Nurilmala Maulana, Fajar Megawati Wijaya Meillisa Carlen Mainassy Melta Rini Fahmi Mia Setiawati Mochamad Syaifudin, Mochamad Mohammad Mukhlis Kamal MUHAMMAD AGUS SUPRAYUDI Muhammad Cito Khairel Fauzan Muhammad Junior Zairin Muhammad Muttaqin Muhammad Zairin Jr Muhammad Zairin Jr Muhammad Zairin Jr. Muhmmad Agus Suprayudi Mulyani, Lina Muslim Muslim N. Suhenda Nadia Mega Aryani, Nadia Mega Nazar, Danella Austraningsih Puspa Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Odang Carman Olivia Karo Permana, Andre Puji Hastuti, Yuni Pustika Ratnawati Putri Utami, Putri Raden Roro Sri Pudji Sinarni Dewi Raden Roro Sri Pudji Sinarni Dewi Raden Roro Sri Pudji Sinarni Dewi Rahmi, Kurnia Anggraini Ratu Siti Aliah Rezki, Dinda Wahyu RIDWAN AFFANDI Rizsa Mustika Pertiwi Ronny I. Wahju S. Sarah Samara, Syifania Hanifah Sandra, Aan Aryanti Sholihin, Hidayatush Sinansari, Shofihar Siti Subaidah Sri Nuryati Sri Nuryati Sufal Diansyah Sularto Sularto Suriansyah Suriansyah Suriansyah, Suriansyah T. Budiardi T. Nursyams T. Prasetya Tatag Budiardi Tuti Puji Lestari Upmal Deswira Uthami Nagin Lestari UTUT WIDYASTUTI Wahyu Purbiantoro Wasmen Manalu Wibowo, Kesit Tisna WIDANARNI WIDANARNI Wildan, Dudi Muhammad Wiwin Kusuma Atmaja Putra Y. Hadiroseyani Yanti Sinaga Yonvitner - Zahri, Abdul

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