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ISOLATION, CLONING AND CHARACTERIZATION OF ACTIN-ENCODING cDNAs FROM Jatropha curcas L. IP-2P Yuniati, Ratna; Widyastuti, Utut; Sopandie, Didy; Yokota, Akiho; Akashi, Kinya; Suharsono, Suharsono
Makara Journal of Science Vol. 15, No. 2
Publisher : UI Scholars Hub

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Abstract

Actin is a major component of the plant cytoskeleton, so all cells contain this protein. Actin is expressed constitutively and is involved in basic housekeeping functions required for cell maintenance. Because of this, it has been frequently used as an internal control to normalize changes in gene expressions analysis. Actually, the information of nucleotide sequence of actin gene of Jatropha curcas L. population IP-2P from Indonesia is not available yet. The objective of this research was to isolate, clone and characterize cDNA of actin genes of J. curcas IP-2P. Three partial actin gene sequences had been successfully isolated by PCR using total cDNA as template, and actin primer designed from conserved region of Arabidopsis thaliana. Nucleotide sequence analysis showed that the length of JcACT fragment is 610, 534, and 701 bp encoding 203, 177, and 234 amino acids respectively. Local alignment analysis based on mRNA sequences shows that JcACT fragment shares 98% similarity with actin mRNA of Hevea brasiliensis and 99% with actin mRNA of Ricinus communis. Based on deduced amino acid sequence, JcACT is 100% identical to actins from Prunus salicina, Gossypium hirsutum, and Betula luminifera. Even though these clones of cDNA are not completed yet, they can be used as reference in J. curcas L. gene expression analysis.
Status Gula dan Hara pada Daun Serta Status Gula pada Buah Manggis Selama Fase Perkembangan Buah Dhika Prita Hapsari; Roedhy Poerwanto; Didy Sopandie; Edi Santosa; Deden Derajat Matra
Jurnal AGROSAINS dan TEKNOLOGI Vol 9, No 1 (2024)
Publisher : Fakultas Pertanian - UMJ

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/jat.9.1.44-52

Abstract

Produksi manggis dari tahun ke tahun mengalami fluktuasi yang cukup signifikan akibat terjadinya ketidakseimbangan fotosintat pada tanaman manggis. Pada saat on year buah manggis yang dihasilkan banyak namun berukuran kecil, sebaliknya pada saat off year buah manggis yang dihasilkan sedikit namun berukuran lebih besar. Selain itu, terjadi gugur buah yang cukup tinggi ketika on year akibat tanaman tidak mampu menopang buah yang sangat banyak. Penelitian ini dilakukan dengan tujuan mendapatkan informasi mengenai status nutrisi pada tanaman manggis sehingga dapat menjelaskan fluktuasi hara dan gula pada tanaman manggis selama satu periode panen. Hasil penelitian menunjukkan bahwa tanaman manggis memiliki rasio jumlah daun dan buah yang tinggi yaitu 142:1. Perubahan kandungan hara daun akibat perkembangan buah manggis paling utama ditemukan pada unsur K, Ca, B, Cu, Zn yang memiliki peran penting dalam proses pembentukan buah. Diduga terjadi hambatan translokasi gula pada fase awal perkembangan buah yang ditunjukkan oleh tingginya kandungan gula pada daun seiring dengan rendahya kandungan gula pada buah manggis. Kandungan glukosa dan fruktosa paling tinggi terdapat pada buah manggis yang memasuki fase pematangan buah.ABSTRACTMangosteen production fluctuates year by year because of imbalance nutrient in plant. Plant produces a lot of fruit with smaller size during “on season”, while few of fruit with bigger size during “off season”. On the other hand, the high fruit drop occurred when “on season” because mangosteen tree is not able to hold too many fruits. This experiment was conducted to obtain the information of nutrient status on mangosteen tree and explain the fluctuation of nutrient and sugar in one harvset period. The result show that mangosteen had a very high ratio between leaves and fruit, i.e 142:1. The changes of nutrient content during fruit development were found in potassium (K) and calcium (Ca), B (boron), Cu (copper), Zn (zinc) which have a critical role in fruit set process. Limitation of sugar translocation in the beginning of fruit development stage was occurred. It shows by the high content of sugar in leaves along with the low content of sugar in mangosteen fruit. The highest glucose and fructose content in mangosteen fruit was found on the beginning of maturation stage.
KARAKTER DAN PRODUKSI TIGA AKSESI TALAS DENGAN PENAMBAHAN DOSIS BAHAN ORGANIK Ridwan Diaguna; Edi Santosa; Trikoesoemaningtyas Trikoesoemaningtyas; Ni Made Armini Wiendi; Didy Sopandie; Sobir Sobir; Eny Widajati
Jurnal Agrotek Tropika Vol 12, No 1 (2024): JURNAL AGROTEK TROPIKA VOL 12, Februari 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i1.6728

Abstract

Perubahan iklim dan keberlanjutan merupakan tantangan besar dalam produksi pangan, yang didominasi sumber pangan biji-bijian yang rentan terhadap perubahan iklim. Sumber karbohidrat umbi dianggap lebih tahan dan mudah beradaptasi dengan tantangan tersebut. Talas merupakan salah satu potensi besar untuk memenuhi kebutuhan pangan dengan nilai gizi yang tinggi. Penelitian bertujuan untuk mengevaluasi pengaruh penambahan dosis bahan organik terhadap karakter morfologi talas, dan hubungan karakter morfologi dan umbi yang dihasilkan, serta menentukan dosis yang tepat untuk budidaya talas di Indonesia. Penelitian menggunakan rancangan acak lengkap faktor tunggal yaitu dosis bahan organik, dengan lima ulangan. Dosis bahan organik terdiri dari 4 taraf yaitu 0,25 kg tanaman-1, 0,5 kg tanaman-1, 0,75 kg tanaman-1, dan 1 kg tanaman-1. Bahan organik (BO) meningkatkan pertumbuhan vegetatif, umbi atribut dan produksi bibit. BO meningkatkan pertumbuhan vegetatif kuantitatif. Dosis sekitar 0,5 - 1 kg lubang-1 diidentifikasi sebagai dosis yang tepat untuk pertumbuhan vegetatif, umbi atribut dan produksi bibit talas. Ada korelasi positif antara pertumbuhan vegetatif, atribut umbi dan produksi bibit. Tinggi tanaman, ukuran daun, dan diameter batang sangat penting untuk menentukan perkembangan umbi, dan selanjutnya menyebabkan tingginya produksi bibit.
Utilizing Rice Hull Ash and Biomass as Ameliorants Enhanced Soybean Yield and Nutrient Uptake in Tidal Swamps Under Saturated Soil Culture Toyip, Toyip; Ghulamahdi, Munif; Sopandie, Didy; Aziz, Sandra Arifin; Sutandi, Atang; Jarwadi Purwanto, Mohamad Yanuar
PLANTA TROPIKA Vol 12, No 1 (2024)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v12i1.18486

Abstract

Saturated soil culture (SSC) is a promising technology for cultivating soybeans in acidic soil in tidal swamps. The objectives of this study were to measure nutrient uptake in the soybean plant using a saturated soil culture technique and to assess the impact of ameliorant addition in the form of rice biomass and rice hull on soybean yield. Two factors, rice biomass, and rice hull ash, were used in a split plot factorial design to set up the experiment. Four levels in the main factor included without biomass immersion, one immersion of short (3 cm above the root) rice stubble, one immersion of regular (panicle harvested only) rice straw, and two immersions of regular rice straw cut in the soil. The four levels in the subfactor were 0 t ha-1, 0.25 t ha-1, 0.50 t ha-1, and 0.75 t ha-1 of rice hull ash doses. Ameliorant in the form of biomass and rice husk increases soil quality in tidal swamps by enhancing pH, soil nutrients (N, P, K, S), CEC, cations, and base saturation, also reducing toxic compounds and improving soil texture. Application of ameliorant also increases soybean nutrient uptake and eventually increases the productivity of soybeans in tidal swamp acid soil. The best treatment of the first and second factors was one immersion of biomass with a regular cut of rice straw and 0.5 t ha-1 ash rice hull ash addition to the tidal swamp soil, respectively. 
Diversity of 17 Genotypes of Taro Based on Anatomy and Nutritional Value of Tuber Cahyanti, Lutfy Ditya; Didy Sopandie; Edi Santosa; Heni Purnamawati
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.465-473

Abstract

Indonesia is one of the countries with the greatest diversity of taro variety, while the study of taro's morphology and nutritional characteristics is limited. The aim of this study was to evaluate the anatomical of plant and nutritional value of taro tuber. This research observed fourteen genotypes of Colacasia esculenta (7 Eddoe types, 7 Dasheen types) and 3 Xanthosoma genotypes. In three blocks of replications, the eddoe and Xanthosoma genotypes were cultivated using cormel and the dasheen genotypes with sucker. Plant anatomy (leaf, stem, and root) and tuber nutritional characteristics were analyzed. From this research, explained that stomata and epidermis number, length from stomata and epidermis, number leaf epidermis, length of leaf and stem epidermis, also length of root epidermis, endodermis, cortex, and stele among 17 genotypes of taro were statistically different. The diversity of taro based on the nutrition content of tuber between 17 genotypes of taro was considered high, as represented by water, ash, fat, protein, carbohydrate, energy, and glucomannan, which were statistically different.
Pengelolaan Pemupukan Kelapa Sawit (Elaeis guineensis Jacq.) di Kebun Aek Nabara, Sumatera Utara Sinaga, Falencia; Sopandie, Didy; Santosa, Edi
Buletin Agrohorti Vol. 12 No. 3 (2024): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v12i3.51531

Abstract

Pengelolaan pemupukan pada kelapa sawit (Elaeis guineensis Jacq.) diperlukan untuk mempertahankan dan meningkatkan produksi. Penelitian berlangsung pada bulan Januari hingga Mei 2021 di kebun Aek Nabara. Penelitian bertujuan menganalisis dan mengevaluasi pengelolaan pemupukan yang mencakup efisiensi dan efektivitas pemupukan. Pengamatan dan pengumpulan data yang dilakukan yaitu dengan metode langsung (data primer) yaitu pengamatan 5T (tepat jenis, dosis, cara, waktu dan tempat), pengelolaan distribusi pupuk dan kehilangan pupuk dan metode tidak langsung (data sekunder). Hasil pengamatan menunjukkan bahwa aplikasi pemupukan di afdeling IV telah sesuai dengan rekomendasi Departemen Penelitian dan Pengembangan. Jenis pupuk yang diaplikasikan, yaitu pupuk AC, RP, MOP, Borat, Dolomit, dan Janjangan kosong. Ketepatan cara aplikasi MOP mencapai 92.78%, yang dikategorikan sangat baik berdasarkan standar kebun. Rata-rata ketepatan lokasi aplikasi pupuk adalah 55.69 cm, sesuai dengan standar kebun (50-100 cm) untuk tanaman menghasilkan <8 tahun. Namun, ketepatan dosis aplikasi MOP hanya mencapai 80.56%, masih di bawah standar kebun yaitu sebesar 92%. Pengawasan dan perencanaan dalam proses pemupukan masih perlu ditingkatkan, khususnya dalam hal dosis pupuk, untuk menghasilkan pemupukan yang optimal. Kata kunci : efisiensi, efektivitas, kehilangan pupuk, ketepatan pemupukan
Promoting Sustainable Sorghum Production: The Role of Ratoon Cultivation and Fertilizer Management Mahpuzah, Marfiatun; Sopandie, Didy; Wirnas, Desta; Trikoesoemaningtyas
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.47-58

Abstract

This study investigated the biomass productivity of sorghum main crop, first ratoon, and second ratoon. A randomized complete block design was employed for the main crop experiment, with eight sorghum genotypes (NS2-19, NS2-102, NS2-109, NS2-111, NS2-140, IPB 4, IPB 8, and “Numbu”) as the treatment factor. The ratoon crop was designed using a Split Plots Design with two treatment factors and three replications. The first factor was fertilizer treatment on the ratoon crops, with two levels: complete N, P, and K fertilizer (urea 133 kg.ha-1, KCl 100 kg.ha-1, and SP-36 100 kg.ha-1) and N fertilizer only (urea 133 kg.ha-1); this factor was arranged as the main plot. The second factor comprised eight elite sorghum lines, the same as those used in the main crop experiment, arranged as subplots. The results indicated that complete NPK fertilization and N fertilizer alone produced similar biomass productivity in sorghum ratoon crops. Among the genotypes, NS2-140 demonstrated the highest biomass productivity in the main crop, NS2-109 in the first ratoon, and NS2-19 in the second ratoon. The second ratoon exhibited the highest biomass yield compared to the main crop and the first ratoon.
Uji Daya Hasil Pendahuluan Beberapa Galur Mutan Padi Gogo Situgintung di Lahan Kering Utami, Nita Nur; Sopandie, Didy; Dewi, Azri Kusuma
Buletin Agrohorti Vol. 13 No. 1 (2025): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v13i1.61619

Abstract

Peningkatan jumlah penduduk setiap tahun menuntut adanya peningkatan produksi padi untuk memenuhi kebutuhan pangan. Budidaya padi gogo menjadi upaya yang dapat dilakukan untuk meningkatkan produksi padi di Indonesia dengan mengoptimalkan penggunaan lahan kering. Penelitian ini bertujuan untuk mengidentifikasi daya hasil dan komponen hasil dari 10 galur mutan padi gogo Situgintung. Penelitian dilaksanakan di Kebun Percobaan Pusat Riset Teknologi Proses Radiasi (PRTPR), ORTN-BRIN, Lebak Bulus, Jakarta Selatan pada bulan Januari hingga Agustus 2023. Penelitian ini menggunakan faktor tunggal yaitu genotipe sebagai perlakuan yang disusun dalam rancangan kelompok lengkap teracak dengan 3 ulangan. Uji beda nyata jujur digunakan untuk mengetahui perbedaan antar perlakuan. Hasil penelitian menunjukkan bahwa seluruh galur mutan yang diuji memiliki produktivitas yang sama dibandingkan dengan dua varietas pembanding Limboto dan Situgintung, kecuali galur G107 dan G29 yang lebih tinggi dibandingkan kedua varietas pembanding serta galur G229 juga nyata lebih tinggi dibandingkan dengan varietas Situgintung. Beberapa karakter menunjukkan korelasi positif terhadap produktivitas galur mutan padi gogo, meliputi lebar daun bendera, panjang batang, jumlah anakan produktif, jumlah anakan total, jumlah gabah bernas, persentase gabah bernas, jumlah gabah total, dan kerapatan gabah per malai. Karakter yang berkorelasi negatif terhadap produktivitas adalah persentase gabah hampa. Kata kunci: padi gogo, galur mutan, produktivitas
Genetic Diversity of 17 Genotypes of Taro Based on Morphological Characteristics Cahyanti, Lutfy Ditya; Santosa, Edi; Sopandie, Didy; Purnamawati, Heni
AGRIVITA Journal of Agricultural Science Vol 47, No 3 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i3.3929

Abstract

This study aims to identify the morphological characteristics of 17 taro genotypes. The research involved the inventory and morphological identification of taro genotypes in Bogor, West Java Province, and Ponorogo, East Java Province. The morphological description of taro followed the guidelines in the IPGRI descriptor book. Analysis of variance (ANOVA) was used to examine quantitative data. Using the 22-0 version of SPSS Software, Duncan’s multiple range test was employed at a 5% significance level to determine whether the variables observed had a significant effect. A cladogram was created to evaluate the degree of similarity between the taro genotypes based on the collected data. Fourteen genotypes of Colacasia have been identified, consisting of 7 Eddoe types: Satoimo, Ozikawa, Siromi, Jepang Ungu, Dempel, and Dempel Ungu, 7 Dasheen types including Bentul Ungu, Ketan, Pratama, California, Sutra, Pari, and Bentul, while 3 Xanthosoma including Talas hitam, Talas HIjau, and Talas Kuning. The results showed that the highest association coefficient values were found in Pratama with California, Bentul Ungu with Ketan, and Ozikawa with Siromi. The genotypic variance coefficients were high for characters associated with taro's growth and yield, including corm weight, cormel diameter, cormel weight, and plant span.
Plant Growth, Nitrogen Content, Nitrogen Uptake, and Seed Quality in Six Bambara Groundnut (Vigna subterranea (L.) Verdc) Landraces Rosyad, Astryani; Ilyas, Satriyas; Qadir, Abdul; Suhartanto, M Rahmad; Sopandie, Didy
Journal of Tropical Crop Science Vol. 12 No. 03 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.03.572-583

Abstract

Bambara groundnut (Vigna subterranea (L.) Verdc) is recognized for its nutritional benefits and ability to improve soil fertility through nitrogen fixation. This study evaluated growth performance, nitrogen dynamics, and seed quality across six landraces: Tasikmalaya, Sukabumi, Sumedang, Small Sumedang, Bogor, and Gresik. The experiment was conducted at IPB University’s Sawah Baru experimental field from July 2022 to March 2023. The experiment used a randomized complete block design with four replications. The measured variables were petiole length, number of petioles, number of leaves, leaf greenness, plant dry weight, nitrogen content, nitrogen uptake in roots, petioles, leaves, and pods, protein content in pods, and seed quality. Results showed significant variation among landraces. Gresik exhibited superior vegetative growth and dry biomass, while Tasikmalaya had the highest pod nitrogen (4.58%) and protein content (28.03%), indicating efficient nitrogen assimilation. Nitrogen analysis revealed distinct uptake patterns across landraces, particularly with Gresik, where a rapid increase in nitrogen suggests that this landrace is well-suited to environments where nitrogen is efficiently fixed and mobilized toward reproductive growth. Sukabumi and Bogor showed the highest germination rates (94%), and Bogor and Gresik had the highest vigor indices (76% and 72%). Overall, Gresik is recommended for balanced performance in growth and seed vigor, while Tasikmalaya is optimal for protein yield and nitrogen use efficiency.
Co-Authors , Hamin , Kisman , Purwono , Suharsono , Syafruddin , Tasliah . SUHARSONO . SUWARNO A. HAITAMI A.A. Ketut Agung Cahyawan W Abd. Aziz Syarif ABDUL KARIM MAKARIM Abdul Munif Abdul Qadir Adolf Pieter Lontoh, Adolf Pieter Agus Purwito AHMAD JUNAEDI AKIHO YOKOTA AKIHO YOKOTA Akiho Yokota Aksarah Pas, Aris Alce Ilona Noyaa Alifiya Herwitarahman Amris Makmur Amrullah Amrullah Anas D Susila Anas Dinurrohman Susila ANGELITA PUJI LESTARI Angelita Puji Lestari Annisa Fadila Ardie, and Sintho Wahyuning Ardie, dan Sintho Wahyuning Arvita Netti Sihaloho, Arvita Netti ASMARLAILI SAHAR Atang Sutandi Azri Kusuma Dewi Azri Kusuma Dewi Budi Mulyanto Careca Sepdihan Rahmat Hidayatullah Chairani Hanum Chairani Hanum Christoph Leuschner Deden Derajat Matra Desta Wirnas Dhika Prita Hapsari DWI ANDREAS SANTOSA Edi Santosa Edi Santosa Edi Santosa Efendi, Darda Enny Sudarmonowati Enny Sudarmonowati Enung Sri Mulyaningsih Enung Sri Mulyaningsih Eny Widajati Erma Prihastanti Fadliah Salim Faqih Udin Gusmaini Gusmaini GUSMAINI GUSMAINI GUSMAINI GUSMAINI, GUSMAINI HAJRIAL ASWIDINNOOR Hajrial Aswidinoor Halimatus Syahdia Hasibuan Hamim Hamim Hanedi Darmasetiawan HANNA ARTUTI EKAMAWANTI Hapsoh Hidayah, Agus Nur I Ketut Suada IBNUL QAYIM Ida Hanarida Soemantri Ika Mariska Ika Mariska Ika Mariska Ilona Noyaa, Alce Imam Widodo Inez Hortense Slamet Loedin Inez Hortense Slamet Loedin Inez Hortense Slamet-Loedin INEZ HORTENSE SLAMET-LOEDIN Iskandar Lubis Joko Prasetiyono Joko Prasetiyono Juang Gema Kartika K. Idris Karlin Agustina Kartika Ning Tyas Ketty Suketi KINYA AKASHI KINYA AKASHI Kisman Kisman Komaruddin Idris Komaruddin Idris La Muhuria Laela Sari Laela Sari laela Sari, laela Lukman Chakim Luluk Setyaningsih Lutfy Ditya Cahyanti, Lutfy Ditya M A Chozin M. A. Chozin Maemonah, Maemonah Mahpuzah, Marfiatun Mariska, Ika Masdiar Bustamam Masdiar Bustamam Masumi Moritsugu Matra, Deden Derajat Maya Melati Mayang Sari Meliala, Merry Gloria Moh. Yani Momongan, Jorex Daniel Muhamad Syukur Muhammad Arif Yudiarto Muhammad Jusuf Munarti Munif Ghulamahdi Muzuni, Muzuni Nani Heryani Nasution, Annisa Andrini Ni Made Armini Wiendi Nurliani Bermawie Nurliani Bermawie NURLIANI BERMAWIE Nurul Khumaida Osmat Azzam Jefferson Osmat Azzam Jefferson, Osmat Azzam OuwerkerV, Pieter BF Pieter B.F. Ouwerkerk Pieter BF OuwerkerV Putri Andini Mandasari RADITE TISTAMA Ragapadmi Purnamaningsih RAGAPADMI PURNAMANINGSIH Ratna Yuniati Rini, Erin Puspita Roedhy Poerwanto Rossa Yunita Rossa Yunita Rossa Yunita Rosyad, Astryani S. Suharsono Saepudin, Adam Sandi, Yusri Iryas Sandra Arifin Aziz Saragih, Ery Leonardo Sari, Laela Sarsidi Sastrosumarjo SATRIYAS ILYAS Satya Nugroho Sinaga, Falencia Sinaga, Parlin H. Sintho Wahyuning Ardie Siti Marwiyah Slamet Loedin, Inez Hortense Sobir Sobir SOEKISMAN TJITROSEMITO Soeranto Human Sri Mulatsih SRI RAHAYU Sri Setyati Harjadi Sri Wilarso Budi Sudarmonowati, Enny Sudirman Yahya Sudirman Yahya Sugianta Sugianta Sugianta, Sugianta SUGIONO MOELJOPAWIRO Sugiono Moeljopawiro Suharsono . Suharsono Suharsono Suharsono Suharsono Suhartanto, Muhammad Rahmad Sumiati, dan Sungkono Sungkono Supijatno Surjono Hadi Sutjahjo Suwarno Suwarno Suwarno Suwarno Suwarto Suwarto Syamsidah Rahmawati Syamsidah Rahmawati, Syamsidah T. Kawasaki T. M. H. Oelim Tanari, Yulinda Tintin Suhartini Titin Handayani Toshio Kawasaki Toyip Toyip TRI KOESOEMANINGTYAS Tri Lestari Trikoesoemaningtyas Utami, Nita Nur UTUT WIDYASTUTI UTUT WIDYASTUTI Utut Widyastuti Utut Widyastuti Suharsono Wage Ratna Rohaeni Wahju Q. Mugnisjah Wahju Qamara Mugnisjah Yadi Setiadi Yadi Setiadi Yadi Setiadi YADI SETIADI Yonny Koesmaryono Yopie Moelyohadi Yuli Sulistyowati YULIN LESTARI Yuniati, Ratna Yunita, Rossa