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All Journal ACTA VETERINARIA INDONESIANA Jurnal Pendidikan Vokasi Jurnal Veteriner Jurnal Ilmu Ternak International Journal of Integrated Health Sciences Majalah Kedokteran Bandung Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Farmasi Klinik Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Jurnal Edik Informatika : Penelitian Bidang Komputer Sains dan Pendidikan Informatika Jurnal Bisnis, Manajemen, dan Informatika JETT (Jurnal Elektro dan Telekomunikasi Terapan) JURNAL ILMIAH PETERNAKAN TERPADU Creative Research Journal ARSHI Veterinary Letters J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Nutrisi Ternak Tropis dan Ilmu Pakan ZIRAA'AH MAJALAH ILMIAH PERTANIAN At-Tajdid : Jurnal Pendidikan dan Pemikiran Islam Media Kontak Tani Ternak Jurnal Farmasi dan Sains Indonesia (JFSI) Journal of Science and Education (JSE) Jurnal PTI (Jurnal Pendidikan Teknologi Informasi) PAKDEMAS : Jurnal Pengabdian Kepada Masyarakat Jurnal Pengabdian Masyarakat Bestari (JPMB) Arcitech: Journal of Computer Science and Artificial Intelligence Journal of Applied Business and Technology Jurnal Kedokteran Hewan Farmers: Journal of Community Services The Indonesian Journal of Computer Science Jurnal Agripet ORAHUA : Jurnal Pengabdian Kepada Masyarakat Journal of Social and Community Development Journal of Medicine and Health
Rini Widyastuti
Laboratorium Reproduksi Dan Inseminasi Buatan, Departemen Produksi Ternak, Fakultas Peternakan, Universitas Padjadjaran
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IMPROVING EWE OOCYTE VIABILITY AFTER VITRIFICATION WARMING USING COMBINATION OF DIFFERENT CRYOPROTECTANT AND CARRIER SYSTEM Widyastuti, Rini; Setiawan, Rangga; Syamsunarno, Mas Rizky Anggun Adipurna; Ghozali, Mohammad; Saili, Takdir; Boediono, Arief
Jurnal Kedokteran Hewan Vol 12, No 3 (2018): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i3.11398

Abstract

The aim of this study was to determine the best combination of cryoprotectant (Ethylene glycol, EG), dimethyl sulfoxide (DMSO) and propanediol (PrOH) and carrier system (hemistraw and cryotop) in improving ewe oocytes viability during cryopreservation. Oocytes with multi layers of compact cumulus cells were colleted from abbatoir and matured in TCM 199 medium supplemented with 10% fetal calf serum for 24-26 h at 38.5 C under 5% CO2 in the air. Matured oocyte was divided into six parts and vitrified in three different vitrification solutions; (i) 17% EG+17% DMSO with hemistraw as carrier system, (ii) 34% EG with hemistraw as carrier system, (iii) 17% EG+17% PrOH in hemistraw (iv), 17% EG+17% DMSO with cryotop as carrier system (v), 34% EG with cryotop as carrier system (vi), and 17% EG+17% PrOH in cryotop. Oocytes were cryopreserved for one week before revived and evaluated for viability. The result showed that oocytes vitrified in media containing EG and DMSO in cryotop had the highest viability (88.16%) compared to media containing EG only or EG and PrOH (70.95% and 68.76%, respectively) (P0.05). Moreover, oocytes viability that vitrified using cryotop and hemistraw as carrier system were not significantly different. The present results indicated that vitrification using combination of EG and DMSO as permeable cryoprotectant and cryotop as carrier system was the best system to maintain oocyte viability after vitrification-warming.
Effect of PMSG administration in combination with vaginal sponge on estrous occurrence and litter size of Javanese sheep Setiawan, Rangga; Rasad, Siti Darodjah; Solihati, Nurcholidah; Widyastuti, Rini; Soeparna, Soeparna
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.4349

Abstract

AbstractThe use of vaginal sponge in estrous synchronization has been a popular method to uniform estrous cycle of small ruminants. In order to increase the litter size, the use of the sponge is combined with PMSG. Therefore the objective of this study was to evaluate the effect of PMSG in combination with vaginal sponge on estrous response and litter size of Javanese local sheep. The study was conducted in Faculty of Animal Husbandry, Padjadjaran University, Indonesia with 32 non-pregnant local sheep. All these sheep was divided into four groups of treatment, i.e: 1) group of sheep was subjected to vaginal sponge (VS) treatment containing 30 mg Medroxyprogesterone acetat (MAP) inserted in the vagina for 14 days; 2) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection -1 d before VS removal; 3) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection at the day of VS removal; 4) group of sheep was subjected to the 30 mg MAP in VS plus 300 IU PMSG injection +1 d after VS removal. The result showed that all treatment groups had 100% of estrous response. However, the occurrence of estrous time varied among group after VS removal. Group 1 (G1) and G4 tend to have estrous occurrence at day 3 after VS removal by 55% and 75% response, respectively. Whilst, G2 and G3 tend to have estrous occurrence respectively at day 1 (62,5%) and day 2 (50%) after VS removal. A 100% of pregnancy rate occurs in G2 and G4, while G1 and G3 have a 88,9% and 87,5%, respectively. No significant effects of the treatments on the ewes litter size. In conclusion, the use of PMSG administration 24 h prior, after, or at sponge withdrawal increase a tighter synchrony of oestrous and pregnancy rate, but has no effect on the increasing of litter size in Javanese local sheep.Keywords: Vaginal sponge, PMSG, estrous response, pregnancy rate, litter size, sheep
Prebiotic Effect of Cogon Grass Root Extract on Stimulating Lactic Acid Bacteria Growth in Mice Intestine Pradini, Gita W; Fauziah, Nisa; Asarina, Shinta; Anggareni, Neni; Widyastuti, Rini; Syamsunarno, Mas Rizky AA
Journal of Medicine and Health Vol 6 No 1 (2024)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28932/jmh.v6i1.7689

Abstract

Lactic acid bacteria (LAB) are known for their numerous beneficial effects for health and their colonization within intestines is affected by prebiotic consumption. Cogon grass root is an herbal medicine which contain substantial quantities of polyphenols. Recently, many studies suggested polyphenols as a novel group of prebiotics. Therefore, this study aimed to explore prebiotic effect of cogon grass root extract (CGRE) on stimulating growth of LAB in mice intestine. Mice (n =7/group) were divided into three groups; group A (control), group B and C received CGRE orally with daily dosage 90 and 115mg/kg BW, respectively. After 14 days of treatment, all mice were sacrificed. LAB was isolated and counted from ileum. Additional biochemical tests were performed to confirm the bacteria as LAB. Further identification of representatives LAB was performed with API-CHL-50 System. We found increased growth and diversity of LAB in the treatment group with optimum growth stimulation at CGRE dosage 90mg/KgBW daily (group B). Further identification showed Lactococcus lactis was the most frequent LAB found in all groups, with additional Lactobacillus paracasei and Lactobacillus curvatus were found in the treatment group. These findings support additional benefit of CGRE as prebiotics by stimulating LAB growth and diversity in mice intestine.
The influence of experience and confidence on the health management of dairy goat (Case study: “Simpay Tampomas Farmer Group” Village Cilengkrang, Cimalaka District of Sumedang, West Java-Indonesia) Hartady, Tyagita; Widyastuti, Rini
ARSHI Veterinary Letters Vol. 2 No. 3 (2018): ARSHI Veterinary Letters - Agustus 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.807 KB) | DOI: 10.29244/avl.2.3.49-50

Abstract

Dairy goat is profitable object since the productivity is relatively brief, easy in maintenance and does not require much investment. Dairy goat become one of the important commodity for farmers in the village Cilengkrang, Cimalaka District of Sumedang. This study used a qualitative approach where 18 farmers who become interviewees. Collected data were observed and compared with the evaluation on the participants’ routine. The majority farmers are depending on individual experiences and conclusions without related guidance and supported references of diseases and procedures in nurturing dairy goats (61.1%), while the rest are consulting the problem to the vet or the group (83,3%). Some of them used the conventional treatment. However, when the condition of sick animal never improved, only 83,3% of farmers who contact veterinarian and the rest would prefer sell the animal in the market. It can be concluded that the majority farmers in the village, has experienced to overcome certain diseases of dairy goats. Nevertheless, the knowledge and sources of supporting references are still limited that affect to the use of inappropriate traditional herbs or regular human medicines from nearby stalls is still relatively high, so the awareness and knowledge of breeders about health management of dairy goat of breeders needs to be improved.
Pengaruh Keberadaan Corpus Luteum terhadap Kualitas dan Tingkat Maturasi Oosit Domba Lokal Umur Pubertas Awal Secara In Vitro Widyastuti, Rini; Syamsunarno, Mas Rizky Anggun Adipurna; Yusuf, Alvin; Ridhlo, Muhammad Rosyid; Prastowo, Sigit
Jurnal Agripet Vol 18, No 2 (2018): Volume 18, No. 2, Oktober 2018
Publisher : Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v18i2.12103

Abstract

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh keberadaan corpus luteum (CL) pada ovarium domba umur pubertas awal terhadap kualitas oosit hasil koleksi dan tingkat maturasinya secara in vitro (IVM). Sebanyak 279 oosit digunakan, terbagi pada kelompok tanpa CL (CL-) sebanyak 143 dan dengan CL (CL+) sebanyak 136. Oosit dipilih berdasarkan homogenitas sitoplasma dan dikelompokkan sesuai jumlah lapisan sel kumulus yaitu grade 1 (4 lapis), grade 2 (34 lapis) dan grade 3 (02 lapis). Media IVM menggunakan Tissue Culture Media 199 ditambah antibiotik, Follicle Stimulating Hormone, dan 10% Fetal Bovine Serum. Maturasi dilakukan pada inkubator 38,5C, 5% CO2 selama 24 jam. Pasca IVM, tingkat kematangan oosit dievaluasi berdasar kemunculan Polar Body I (PB I). Hasil menunjukkan bahwa keberadaan CL tidak berpengaruh terhadap kualitas oosit yang dikoleksi pada semua grade. Keberadaan CL berpengaruh pada tingkat kematangan oosit pada grade 1 sebesar 48,64% dibandingkan CL- sebesar 47,19% (p0,05). Berdasarkan hasil penelitian disimpulkan bahwa berpengaruh pada tingkat kematangan oosit setelah IVM. Hasil penelitian menggambarkan potensi penggunaan oosit ternak umur pubertas awal untuk digunakan lebih lanjut dalam program produksi embrio secara in vitro.(Effect of the presence of corpus luteum on oocytes quality and in vitro maturation rate of ewes at early puberty)ABSTRACT. This study aims to know effect of presence of corpus luteum (CL) to collected oocytes quality and its maturation rate post in vitro maturation (IVM), on local ewes ovary at early puberty. In total 279 oocytes were collected, 143 without CL (CL-) and 136 with CL present (CL+). Oocytes were selected according to sitoplasma hemogenity, divided into 3 grades according to cumulus cell (CC) layer namely Grade 1, 2 and 3 indicated by 4, 34, and 02 CC layers, respectively. The IVM media was Tissue Culture Media 199 supplemented with antibiotic, Follicel Stimulating Hormone, and 10% Fetal Bovine Serum following culture at 38.5C and 5% CO2. Twenty four hours post IVM, oocytes were evaluated on the presence of Polar Body I. Result showed that oocytes quality were not different among group in all grades. The present of CL gives better maturation rate in grade 1 compared to CL- (48.64 vs 47.19%; p0.05). The present finding show that the presence of CL improves oocytes maturation rate post IVM. Moreover, this study shows the potency of using oocytes from ewes ovary at early puberty for further in vitro embryo production program
Designing Academic Data Visualization Dashboard Using Google Data Studio at SMPN 8 Pariaman M. Dyon; Karmila Suryani; Rini Widyastuti; Ade Fitri Rahmadani
Journal of Applied Business and Technology Vol. 5 No. 1 (2024): Journal of Applied Business and Technology
Publisher : Institut Bisnis dan Teknologi Pelita Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35145/jabt.v5i1.154

Abstract

This study aims to use Google Data Studio to produce an academic data visualization dashboard system to display information, which can manage academic data, namely teacher attendance data and student grades, to provide decision-making support for decision makers in the monitoring process and evaluate school internal processes and monitor student grades at SMPN 8 Pariaman School. The type of research used is the R&D (Research and Development) approach SDLC (Systems Development Life Cycle) method with a prototype model. The stages carried out are communication, planning, modeling, construction and handover (handover). The system testing method studied uses questionnaire guidelines for functional testing to test system functionality to verifiers and usability testing to test system usability and suitability of functions and services to users. Based on the results of the validator functional test study, the results of meeting the functional requirements with both a value of 1 and 89% of the feasibility value of the system usability test are classified as very feasible. Therefore, it can be concluded that the academic data visualization dashboard system using Google Data Studio meets the requirements and is very suitable as a support for SMPN 8 Pariaman School in processing academic data on teacher attendance and student grades.
Co-Authors Abdullah, MT, Dr. Rijal Ade Fitri Rahmadani Ade Irma Suryani Ade Riki Kartana Aida Riyanti Alam, Sri Rahayu Alda Prayoga, Rama Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alkaustariyah Lubis Alvin Yusuf An An Nurmeidiansyah, An An An An Yulianti Andi Hiroyuki Anggareni, Neni Annisa Yusrina Apriana, Diwi Arief Boediono Asarina, Shinta Ashabul Khairi Asmar Yulastri Asri Wulansari, Asri Bayani, Matni Syifa Dahlia Setiawan Dani Garnida Daud, Andre Rivianda Deru Indika Deru R Indika Desi Yunita Desti Ranihusna Diah Nugrahani Pristihadi Diding Latipudin Diky Ramdani dudi, dudi Dumono DWI ANDI NURMANTRIS Dwi Cipto Budinuryanto Dwi Wahyudha Wira Edianingsih, Primiani Elizamiharti Emini, Emini Fadhal M. Ahmad Fadjeri, Indrayati Fahmy Avicenna Faradana, Najma Rika Farel Fairuz Fauziah, Nisa Fauziah, Nisa Fauziati, Popi Firmansyah, Indra Fitri Rahmadani, Ade Ganefri Gea, Yufen Yuliandri Gita Widya Pradini Habibullah, Hafizh Henhen Heryaman Heryawan Kemal Mustafa Husna, Afifah Iin Susilawati Ilham Nugraha Iman Hernaman Inna Kholidasari Insy Nafisah Taufik Ismiraj, Muhammad Rifqi Jaqueline Sudiman Julius Pangayoman Karisma Mardatillah Karmila Suryani Karmila Suryani Kaseri, Kaseri Kasihani, Ni Nyoman Khairudin Khairudin Khoirinaya, Candrani Khotimah, Devi Syahrilia Kikin Winangun Kikin Winangun Krismadinata Krismadinata, Krismadinata Kurnia A Kamil Lubis, Alkaustariyah M. Dyon M. Ghozali M. Rosyid Ridlo Madeleine Priscilia Madeline Priscilla Mansyur . Mas Rizky A. A. Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A Syamsunarno Mas Rizky A.A. Syamsunarno Mas Rizky Adipurna Anngun Syamsunarno Mohamad Ghozali Mohammad Ghozali Mohammad Ghozali, Mohammad Mokhamad Fahrudin Mokhamad Fakhrul Ulum, Mokhamad Muhammad Rifqi Ismiraj Muhammad Rosyid Ridhlo Muhammad Rosyid Ridlo Mulyanusa Ritongga Murlena, Murlena Mushawir, Andi Nahak, Martinus Nelfira Nena Hilmia Neni Anggraeni Neni Anggraeni Nizwardi Jalinus Novi Mayasari Novi Mayasari Novrita, Sri Zulfia Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurcholidah Solihati Nurfitri Handayani, Nurfitri Nurul Ikhwan Pangestu, Muhammad Syahid Panji Petrus Kune Pisriwati, Siti Atwano Pradini, Gita W Priscilla, Madeleine Radial Anwar, Radial Rahmadani, Ade Fitri Rahmat Hidayat Ramdan Panigoro Rangga Setiawan Rangga Setiawan Refdinal, Refdinal Ridhlo, Muhammad Rosyid Ridwan Riska Amelia Rizka Amelia Rizki, Yovan Robianto, Sondi Romi Zamhir Ronnie Permana SATRIYAS ILYAS Sefriani, Rini Sigit Prastowo Sigit PRASTOWO Sigit Prastowo Siswanto, Deny Hadi Siti Darodjah Rasad Siti Darodjah Rasad Siti Darodjah Rasad Siti Darodjah Rasad Soeparna Soeparna Soeparna Soeparna, Soeparna Sondi Robianto SONY HERU SUMARSONO Sony Heru Sumarsono Sony Heru Sumarsono SONY HERU SUMARSONO Sony Heru Sumarsono Sri Restu Ningsih Sri Suwarni Sudiman, Jaqueline Suhaida, Jihan Sukamtono Sukamtono Syamsunarno, Mas Rizky A. A Syamsunarno, Mas Rizky A. A. Syamsunarno, Mas Rizky AA TAKDIR SAILI Toha . Tya Gita Tyagita Hartady Tyagita Tyagita Uni Gamayani Wahyudin Wandi Syahindra Wibowo, Agus Wahyu Winangun, Kikin Winsa, Larasati Yulcherlina Yusuf, Alvin Zein, Syaikha Dziyaulhaq