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Correlation of Vitamin D and Antimullerian Hormone Level in Infertile Women with Polycystic Ovary Syndrome and Obesity Usman, Fatimah; Saleh, Irsan; Effendi, Kms. Yusuf; Putra, Hadrians Kesuma; Abadi, Adnan; Siahaan, Salmon Charles Pardomuan Tua; Kesty, Cindy; Rizda, Dian Permata; Stevanny, Bella; Lestari, Peby Maulina
Indonesian Journal of Obstetrics & Gynecology Science Volume 7 Nomor 3 November 2024
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/obgynia.v7i3.723

Abstract

Introduction: Increasing amount of research indicates that women with PCOS are more likely to have a shortage in vitamin D with abnormally high anti-Müllerian hormone (AMH) levels. This study aims to assess the correlation between vitamin D and Anti-Mullerian Hormone (AMH) levels in infertile women with polycystic ovarian syndrome (PCOS) and obesity. Method: This observational cross-sectional study examined all obese women with PCOS who sought infertility treatment at Dr. Mohammad Hoesin Hospital Palembang from August 2021 to November 2021. PCOS diagnosis was determined using the Rotterdam criteria. All research subjects underwent a physical examination and transvaginal ultrasound to evaluate body mass index (BMI) and polycystic ovary morphology. Blood samples were collected to evaluate their testosterone, AMH, and vitamin D levels. Statistical analysis was performed using the Spearman correlation test with IBM® SPSS Statistics version 24. Results: Thirty infertile women with PCOS and obesity were included in the study, with average age of 31.17±4.48 years and average BMI of 33.45±2.67 kg/m2. The majority of the subjects had polycystic ovary morphology (86.7%) and oligomenorrhea (76.7%). No correlation between BMI and vitamin D as well as AMH levels was found in infertile women with PCOS and obesity. The Spearman test showed moderate negative correlation (p = 0.011. r = -0.458) between vitamin D and AMH levels in infertile women with PCOS and obesity. Conclusion: There is a moderate negative correlation between vitamin D and AMH levels among infertile women with PCOS and obesity. Further research is required to comprehend the role of vitamin D in female fertility.Korelasi Vitamin D dan Kadar Hormon Anti-Müllerian pada Wanita Infertil dengan Sindrom Ovarium Polikistik dan ObesitasAbstrakPendahuluan: Semakin banyak penelitian melaporkan bahwa wanita dengan PCOS cenderung mengalami defisiensi vitamin D dengan penignkatan kadar hormon anti-Müllerian (AMH). Penelitian ini bertujuan untuk mengkaji hubungan vitamin D dan kadar AMH pada wanita infertil dengan sindrom ovarium polikistik (SOPK) dan obesitas.Metode: Penelitian ini merupakan studi observasional potong lintang terhadap seluruh wanita infertil dengan SPOK dan obesitas yang di RSUP Dr. Mohammad Hoesin Palembang pada bulan Agustus 2021 hingga November 2021. Diagnosis SOPK ditegakkan berdasarkan kriteria Rotterdam. Seluruh subjek penelitian menjalani pemeriksaan fisik dan ultrasonografi transvaginal untuk menilai indeks massa tubuh (IMT) dan morfologi ovarium polikistik. Sampel darah dikumpulkan dari semua wanita untuk mengukur kadar testosteron, AMH, dan vitamin D. Analisis statistik dilakukan dengan menggunakan uji korelasi Spearman menggunakan IBM® SPSS Statistics versi 24.Hasil: Tiga puluh wanita infertil dengan SOPK dan obesitas dilibatkan dalam penelitian ini dengan usia rata-rata 31,17±4,48 tahun dan rata-rata IMT 33,45±2,67 kg/m2. Mayoritas subjek memiliki morfologi ovarium polikistik (86,7%) dan oligomenore (76,7%). Tidak ada korelasi antara IMT dan vitamin D maupun kadar AMH pada wanita infertil dengan SOPK dan obesitas. Uji Spearman menunjukkan korelasi negatif sedang (p=0.011.r=-0.458) antara kadar vitamin D dan AMH pada wanita infertil dengan SOPK dan obesitas. Kesimpulan: Kadar vitamin D berkorelasi negatif dengan kadar AMH pada wanita infertil dengan SOPK dan obesitas. Penelitian lebih lanjut diperlukan untuk memahami peran vitamin D pada kesuburan wanita.Kata kunci: Anti-Müllerian Hormone, Indeks Massa Tubuh, Obesitas, Sindrom Ovarium Polikistik, Vitamin D
Potency of Leaf Water Extract Karamunting (Rhodomyrtus tomentosa) for Enhancing GLUT4 Translocation in Diabetic Rats Saleh, Irsan; Sutandar, Vivi Hendra; Amrina; Ningrum, Evi Dodik Novita; Sinaga, Ernawati
Science and Technology Indonesia Vol. 11 No. 2 (2026): April
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2026.11.2.742-749

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Insulin resistance and damage in the pancreas are associated with type 2 diabetes, which is a chronic disease affecting health, with 422 million people affected in 2016 and 1.5 million deaths related to diabetes in 2012. Several proinflammatory mediators, such as TNF-a andIL-6, may be involved in insulin resistance in T2DM. GLUT4 is one of the glucose transporters that help promote glucose uptake. GLUT4 activation is insulin-dependent in regulating glucose uptake. Karamunting (Rhodomyrtus tomentosa (Ait.) Hassk.) leaf water extract consists of alkaloids, flavonoids, triterpenoids, saponins, and tannins. This study proposes to investigate the potential of karamunting (R. tomentosa) leaf water extract to enhance glucose uptake by modulating GLUT4 translocation in the serum and skeletal muscle of diabetic rats. The dose variations used in this study were 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW. Based on the results, oral administration of karamunting water extract increases GLUT4 expression in serum. It increases GLUT4 mRNAlevels in the skeletal muscle of the diabetic rat. It decreases TNF-a and IL-6 expression, with a 100 mg/kg BW dosage being the most effective. Meanwhile, 100 mg/KgBW is the most effective dosage for decreasing HOMA IR. These results indicate that karamunting (R. tomentosa) leaf water is antidiabetic by lowering TNF-a, IL-6, and HOMA IR in diabetic rats while increasing GLUT4.
Hubungan Ekspresi Epidermal Growth Factor Receptor dan Skor Gleason pada Karsinoma Prostat Susilawati Susilawati; Mezfi Unita; Ika Kartika; Irsan Saleh
Majalah Patologi Indonesia Vol. 24 No. 2, Mei 2015
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Latar belakang Kanker prostat merupakan karsinoma terbanyak keenam di dunia dan penyebab kematian tersering kedua pada laki-laki. Epidermal growth factor receptor (EGFR) berperan penting dalam proses karsinogenesis tumor solid termasuk karsinoma prostat. Overekspresi EGFR dapat meningkatkan proliferasi sel tidak terkendali sehingga dapat digunakan sebagai faktor prognostik. Penelitian ini bertujuan untuk mengetahui hubungan ekspresi EGFR dan skor Gleason pada karsinoma prostat. Metode Penelitian ini adalah penelitian observasional potong lintang. Limapuluh sampel yang telah didiagnosa adenokarsinoma prostat terdiri dari 24 sampel jaringan hasil transurethral resection prostate (TURP) dan 26 sampel jaringan hasil prostatektomi. Sampel diambil dari arsip di Bagian Patologi Anatomik RSUP Dr. Mohammad Hoesin Palembang (periode 1 Januari 2010 sampai 31 Desember 2012). Kemudian sampel penelitian dipulas dengan antibodi EGFR, diidentifikasi dan dianalisis hubungan EGFR tersebut dengan skor Gleason. Hasil Positivitas ekspresi EGFR pada adenokarsinoma prostat cenderung lebih banyak dijumpai pada kelompok skor Gleason ≥7 (87,1%) daripada kelompok skor Gleason 0,05). Kesimpulan Tidak ada hubungan bermakna antara ekspresi EGFR dan skor Gleason pada adenokarsinoma prostat. Kata kunci : adenokarsinoma prostat, EGFR, skor Gleason.
MicroRNA-21 Expression in Prostate Cancer: A Systematic Review Maria Ulfa; Krisna Murti; Irsan Saleh; Debby Handayati
Indonesian Journal of Global Health Research Vol 7 No 3 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i3.6042

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Prostate cancer is the second most prevalent and fourth largest cause of death for men globally. Meanwhile, in Indonesia, prostate cancer ranks and is the fourth leading cause of death. Molecular studies continue to be conducted to understand the molecular heterogeneity, both genetic and epigenetic, that affects the progression of prostate cancer, one of which is the role of microRNA. This study aims to analyze the expression profile of microRNA-21 associated with the development and aggressiveness of prostate cancer. Comprehensive search into three databases; PubMed, Cochrane Library, and Science Direct was performed by authors. The study that best met the inclusion criteria was chosen from among duplicates. The QUADAS-2 (Quality Assessment of Diagnostic Accuracy Studies) tool was used to assess the risk of bias before the relevant data were retrieved for analysis. There were eleven studies that included a variety of sample sources, sample size and methods. Although a number of the studies had unclear standard reporting, the research overall quality was acceptable. For the results, Prostate cancer samples showed a significantly higher expression level of miR-21 than controls in ten of the studies, and was linked to cancer aggressiveness. In one study, the expression of miR-21 did not significantly increase, despite the use of extracellular vesicle isolation samples.
The Role of Toll-Like Receptor-2 in the Pathogenesis of Pulmonary Tuberculosis Sudarto Sudarto; Zen Hafy; Irsan Saleh; Iche Andriyani Liberty; Zen Ahmad; Fadhyl Zuhry Lubis; Owen Hu; Welly Salutondok
Indonesian Journal of Global Health Research Vol 7 No 3 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i3.6797

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Pulmonary tuberculosis (PTB), primarily caused by Mycobacterium tuberculosis (M.tb), remains a major global health burden. Toll-like receptor 2 (TLR-2), a critical component of the innate immune system, plays a key role in the host-pathogen interaction by recognizing specific components of the mycobacterial cell wall and initiating downstream inflammatory pathways. However, the dual role of TLR-2 in both protective immunity and immune evasion by M.tb contributes to the complexity of TB pathogenesis. This study aims to investigate the role of Toll-Like Receptor-2 (TLR-2) in the pathogenesis of pulmonary tuberculosis, including its immunological mechanisms, relationships with disease severity, and the potential of TLR-2 as a diagnostic and therapeutic target. This literature review systematically analyzed molecular mechanisms involving TLR-2 signaling in pulmonary TB using peer-reviewed primary and secondary sources from experimental and clinical studies. Emphasis was placed on signal transduction (NF-κB and MAPK), cytokine profiles, antigen presentation, and the impact of TLR-2 gene polymorphisms on TB susceptibility. Activation of TLR-2 through ligands such as lipoproteins, lipoarabinomannan (LAM), and PE/PPE proteins initiates immune responses via MyD88-dependent pathways, leading to the release of proinflammatory cytokines (TNF-α, IL-6, IL-12). TLR-2 also enhances the function of macrophages and dendritic cells, promoting Th1-mediated immunity. However, chronic or excessive stimulation of TLR-2 can suppress antigen processing, promote IL-10 expression, inhibit phagolysosome fusion, and facilitate M. tb survival within host macrophages. Polymorphisms in the TLR-2 gene (e.g., rs3804099) have been associated with increased susceptibility and variable clinical outcomes in PTB. TLR-2 plays a paradoxical role in pulmonary tuberculosis by mediating both protective immunity and facilitating immune evasion by M.tb. Understanding the balance of TLR-2 signaling and genetic variation is crucial for developing immunomodulatory therapies and personalized interventions in TB management.
Clinical and Neurophysiological Features of Vincristine-Induced Peripheral Neuropathy in Indonesian Children with Acute Lymphoblastic Leukemia Raden Muhammad Indra; Irsan Saleh; Irawan Mangunatmadja; Yulia Iriani; Zen Hafy; Dian Puspitasari
Indonesian Journal of Global Health Research Vol. 7 No. 6 (2025): Indonesian Journal of Global Health Research
Publisher : GLOBAL HEALTH SCIENCE GROUP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/ijghr.v7i6.584

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Vincristine-induced peripheral neuropathy (VIPN) is a common complication clinical pattern vary across populations, and data from Indonesian children remain limited. Objectives to describe the clinical and nerve conduction study (NCS) features of VIPN in children with ALL treated at a tertiary hospital in Indonesia. We conducted a cross-sectional study of children with ALL receiving chemotherapy recruited consecutively at Mohammad Hoesin Hospital, Palembang, from November 2023 to August 2025. Data were collected from detailed neurological and NCS examinations. Diagnosis of VIPN was made using NCS. Severity was graded using the Common Terminology Criteria for Adverse Events (CTCAE v5.0). Clinical features and NCS findings were evaluated. Comparisons were made between induction and maintenance phases, including the proportions of severity groups, clinical symptoms, individual nerve involvements, number of symptoms and nerves, as well as the type of lesions. A total of 118 children were enrolled; 65 (55.1%) had VIPN by NCS criteria. Neuropathy incidence was similar in induction (56.9%) and maintenance (52.8%) phases (p=0.66). Most cases were CTCAE grade 2–3. Weakness was the most frequent symptom (56.9%), while paresthesia was significantly more common in the induction phase (p=0.008). Peroneal motor nerve involvement was the most frequent NCS abnormality (91.1%), followed by median motor (50.0%) and ulnar nerves (33.7%). The induction phase was characterized by additional upper extremity nerve involvement compared to the maintenance phase, including the median (p=0.004) and ulnar (p=0.025) nerves. Sural sensory nerve abnormalities were significantly more prevalent during the maintenance phases (p=0.002). All cases demonstrated axonal, motor-predominant neuropathy, with no isolated sensory cases. VIPN is highly prevalent among Indonesian children with ALL, with motor-predominant, axonal involvement. The induction phase has more widespread nerve involvement that includes upper extremities, while the maintenance phase has more sensory sural nerve involvement, albeit more likely asymptomatic or milder. Phase-specific patterns suggest distinct pathophysiological mechanisms.
Pendampingan santri dalam pemanfaatan tanaman obat untuk mencegah anemia Evi Lusiana; Muhammad Irsan Saleh; Nia Savitri Tamzil; Desi Oktariana; Tia Sabrina; Aisya Turridho; Zahrah Aliyah Qonitah
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 6 No 3 (2025): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V6l3.178

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Anemia is a condition characterized by a deficiency of hemoglobin in the blood, which can impair productivity and health, particularly among vulnerable populations such as Islamic boarding school students (santri). Education and the utilization of herbal plants have potential as effective measures for anemia prevention. However, these approaches are underutilized in this population. This study aimed to enhance the knowledge and skills of santri regarding anemia prevention, the use of herbal plants, and health screening at the Modern Thawalib Sriwijaya Islamic Boarding School in Palembang, involving 40 participants (67.5% male, 32.5% female). Activities included educational sessions, health screenings, and guidance on herbal plant cultivation. Evaluations were conducted using pretest and posttest assessments. Results demonstrated significant improvements in knowledge (from 30% to 95%), attitudes (from 15% to 65%), and practices (from 47.5% to 82.5%). Hemoglobin screening revealed moderate anemia in 42.5% of participants, mild and severe anemia in 10% each, and no anemia in 37.5% of participants. Educational interventions effectively improved the understanding, attitudes, and behaviors of santri concerning anemia and the preventive potential of herbal plants.
Crosstalk Apoptosis-Autofagi pada Kanker Serviks: Peran BAX, BCL-2, dan Cleaved Caspase-3 Diah Komala Sari; Mgs Irsan Saleh; Subandrate Subandrate; Shaum Shiyan
Jurnal Biomedika dan Kesehatan Vol 9 No 2 (2026)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2026.v9.207-218

Abstract

Cervical cancer remains a major cause of cancer-related morbidity and mortality among women worldwide. In addition to persistent human papillomavirus (HPV) infection, dysregulation of cell survival and cell death mechanisms contributes to cervical cancer development and progression. Apoptosis and autophagy are two important biological processes involved in regulating cancer cell survival and death; however, the interaction between these processes remains complex and context-dependent. This review aimed to examine the crosstalk between apoptosis and autophagy in cervical cancer, with a particular focus on the roles of BAX, BCL-2, and cleaved Caspase-3. A narrative literature review was conducted using PubMed to identify publications related to apoptosis–autophagy crosstalk in cervical cancer, with particular attention to studies discussing BAX, BCL-2, cleaved Caspase-3, and selected autophagy-related regulators published between 2020 and 2025. Articles were selected based on relevance to the review topic and were synthesized descriptively to provide an overview of current evidence. The reviewed studies indicated that increased apoptotic activity was frequently associated with BAX upregulation, BCL-2 downregulation, and activation of cleaved Caspase-3. In contrast, autophagy exhibited a context-dependent role and functioned either as a mechanism of cancer cell death or as a protective process that promotes cancer cell survival. The interaction between BCL-2 and Beclin1, together with the PI3K/Akt/mTOR signaling pathway, emerged as important components of apoptosis–autophagy crosstalk. The conclusions was the balance between apoptosis and autophagy appears to influence cervical cancer cell responses to therapeutic interventions. BAX, BCL-2, and cleaved Caspase-3 may provide useful molecular information for future investigations of cervical cancer biology and therapeutic responses
Co-Authors Abdi Wijaya Abdulwahab Hakimi Ade Suryaman Aditiawati Aditiawati Adnan Abadi, Adnan Adrian, Ronny Ahmad Rasyid ahmad rasyid, ahmad Ahmad, Zen Aisya Turridho AK Ansyori Akmal, Reza Ali Ghanie Ali Ghanie Ali Ghanie Alif Fathur Rachman, Muhammad Alvilusia, Alvilusia Alwi Shahab Amir Fauzi Amir Fauzi Amrina Andi Putra Siregar Anggeria, Septa Anita Tursia Ansyori, AK Arlis Karlina Aseptianova Aseptianova Athiah, Medina Ayeshah A Rosdah Azhari Azhari Banun, Syahri Bastian Bastian Bastian Bintang Arroyantri Prananjaya Chairil Anwar Chairil Anwar Chakra, Aerul Dalillah Dalillah Debby Handayati Desi Oktariana Dessy Adhriyani, Dessy Diah Komala Sari Dian Puspitasari Dian Wahyuni Dwi Handayani Dwi Handayani Dwirini Retno Gunarti Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim, Eddy Mart Eeng Yoneska Effendi, Ian Effendi, Kms. Yusuf Efman EU Manawan Efman Manawan Efrieni Yusuf Ella Amalia, Ella Emilia Eny Rahmawati, Eny Ernawati Sinaga Erwin Sukandi Erwin Sukandi Evi Lusiana Fadhyl Zuhry Lubis Fatmawati Fatmawati Fatmawati Ferdi, Roni Ferry Usnizar Fransisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fuad Bakry Gita Dwi Prasasty Hafizzanovian, Hafizzanovian Heni Maulani, Heni Herdana, Nova Hermansyah Ian Effendi Iche Andriyani Liberty, Iche Andriyani Ika Kartika Imran Saleh Irawan Mangunatmadja Irfannuddin Irfannuddin Irfanuddin Irfanuddin Istiqomah, Amelia Izzah, Puspita Nurul Jayawarsa, A.A. Ketut Joko Marwoto Jusak Nugraha Kamaluddin Kamaluddin Kartini Wulan Kemas Ya'kub Rahadiyanto Kemas Ya'kub Rahadiyanto Kemas Ya'kub Rahadiyanto Kendenan, Margaretha Kesty, Cindy Kms Yusuf Effendi Krisna Murti Krisna Murti kurniati, nova Kusrini, Ida Kusuma Harimin, Kusuma Legiran Legiran Leni Wijaya, Leni Makbruri Amin Margaretha Kendenan Maria Ulfa Mediarty Mega Sary Mezfi Unita Mudinillah, Adam Muhamad Ayus Astoni Muhammad Hadi Wijaya Muhammad Haryadi Ade Kurnadi Nadia Karimah Amalia Zulfadli Nia Savitri Tamzil Ningrum, Evi Dodik Novita Nita Parisa Nova Herdana Nova Kurniati Nova Kurniati Nova Kurniati Nova Kurniati Novadian Novadian Novadian, Novadian Nugroho, Suroso Adi Nur Riviati, Nur Nuswil Bernolian Oktariana, Desi Owen Hu Pahlawan, M Kaisar Patwa Amani, Patwa Peby Maulina Lestari, Peby Maulina Phey Liana Poedji Loekitowati Hariani Purwoko, Izazi Hari Puspa Zuleika, Puspa Putra, Hadrians Kesuma Rachmat Hidayat RACHMAT HIDAYAT Raden Muhammad Indra Radiyati Umi Partan Rahadiyanto, Kemas Ya'Kub Rahadiyanto, Kemas Ya’kub Ramadhan, Philosophia Ramzi Amin Ramzi Amin, Ramzi Ratih Pratiwi Reza Akmal Riani Erna Rieke Cahyo Budi Utami Rizal Zainal Rizani Amran Rizda, Dian Permata Rizki Andini Nawawi Ronny Adrian Rostika Dewi Rukiah Chodilawati Rukiah Chodilawati Sabri Prihantika Sabrina, Tia Safyudin Salmon Charles Pardomuan Tua Siahaan Salni Salni Salni Sari, Febrina Sary, Mega Sastradinata, Irawan Shahab, Alwi Shaum Shiyan Soenarto Kartowigno Sri Nita Sri Nita Sri Nita, Sri Stevanny, Bella Subandrate Sudarto Sudarto Sulfa Esi Warni Suprapti Suprapti Suprapti Suprapti Susilawati Susilawati Sutandar, Vivi Hendra Syamsu Indra Syifa Alkaf Tanoehardjo, Francisca Srioetami Tanoehardjo, Francisca Srioetami Tanzil, Iswadi Tati Lahmuddin, Tati Tatit Nurseta Taufik Indrajaya Taufik Indrajaya Taufik Indrajaya Taufik Indrajaya Taufik Indrajaya Tengku Arfi Sulaiman Theodarus, Theodarus Theodorus Theodorus Tiara Fatrin Triwani Triwani Triwani Triwani Tursia, Anita Usman Said, Usman Usman, Fatimah Veny Larasati Welly Salutondok Widyastuti Wina Safutri Wresnindyatsih Wresnindyatsih, Wresnindyatsih Yeni Agustin Yeni Agustin Yenny Dian Andayani Yesi Astri Yudhie Tanta Yulia Farida Yahya Yulia Iriani Yundari, Yundari Zahrah Aliyah Qonitah Zen Ahmad Zen Ahmad Zen Hafy Ziske Maritska Zulfadli, Nadia Karimah Amalia Zulfiawan, Zulfiawan Zulkarnain Musa, Zulkarnain Zulkhair Ali Zulkhair Ali Zulkhair Ali Zulkifli Zulkifli Zulkifli Zulkifli