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YUSNITA YUSNITA
Jurusan Peternakan Fakultas Pertanian Universitas Bengkulu Jl WR Supratman Kandang Limun Bengkulu Telp 21170 ext 219
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In Vitro Growth and Development of Progenies Derived from Crossings of Several Dendrobium Accessions Under Different Media Maulida, Desi; Yusnita, Yusnita; Hapsoro, Dwi; Agustiansyah, Agustiansyah; Karyanto, Agus; Hamiranti, Rahmadyah; Nugroho, Rizki Bagus
AGRIVITA Journal of Agricultural Science Vol 47, No 2 (2025)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v47i2.4505

Abstract

The objective of plant hybridization is to generate alternative superior phenotypes with the aim of decreasing the dependence on wild orchid exploitation in nature. Although interspecies crosses in Dendrobium are very likely to occur and there is a low chance of experiencing bud depression, interspecies crosses have the potential to encounter several problems, including failure of embryo development resulted to the seeds unable to germinate. Media formulation is the most essential component in the success of in vitro culture of orchid seeds. Therefore, this study aimed to obtain new Dendrobium hybrids and the best media formulation for germination and seedling growth. A total of three experiments were conducted including hybridization, the impacts of basic media namely (1) MS, (2) VW, and (3) foliar fertilizer both with and without the addition of tryptone, on seedling germination. The results showed that there was no compatibility barriers observed in all crosses. Foliar fertilizer + tryptone media was recommended for orchid seedlings because it produced better growth and roots up to 8 months after planting (MAP). For best results on Dendrobium hybrid seedlings, MS + tryptone media up to 6 MAP was used followed by deflasking into VW + tryptone to stimulate optimal root growth before hardening.
SCALP AND SHOOT FORMATION IN RESPONSE TO THIDIAZURON CONCENTRATIONS IN VITRO CULTURE OF PLANTAIN ‘TANDUK’ Hapsoro, Dwi; Prayogi, Adi Noor; Ramadiana, Sri; Yusnita, Yusnita
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.9927

Abstract

This experiment aimed to study the formation of scalps and  banana shoots 'Tanduk' in vitro in response to thidiazuron (TDZ). Shoot explants measuring ± (1 x 1 x 1) cm3 were cultured in precondition media (MS+ 5 mg/l benzyladenine) for 4 weeks, then transferred to treatment media, namely MS + TDZ. This experiment was carried out using a completely randomized design with 3 replications and 7 treatments of TDZ concentrations (0.5; 1.0; 1.5; 2; 2.5; 3; 3.5; 4.0 mg/l).  Each experimental unit consisted of 5 bottles, each containing one explant. Observations of scalp number were carried out at 8 weeks of age. The scalps were subcultured on MS+5mg/l BA media to induce shoots. After 8 weeks in the shoot induction medium, the number of shoots was recorded.  The results showed that at 8 weeks on the culture all of the explants formed scalps, with the highest number of scalps (6 scalps/explant) was at 1 mg/l TDZ.  The scalp number decreased to 4.4 to 3.5/explant as the TDZ concentration increased from 1.5 to 4 mg/l. After 8 weeks on shoot induction media containing 5 mg/l BA, the highest number of shoots (3.8 shoots/explant) was obtained on the scalp from the 0.5 mg/l TDZ treatment. The shoot number decreased to (3.2-1.9/explant) with increasing TDZ concentrations from 1.0 to 4.0 mg/l.Keywords: Tanduk, plantain, thidiazuron, in vitro, scalp.
EMBRIOGENESIS SOMATIK UBI KAYU (Manihot esculenta CRANTZ.) KLON WAXY PADA BEBERAPA JENIS DAN KONSENTRASI AUKSIN 2,4-D ATAU PICLORAM DAN NAA Erlangga, Wahyu; Yelli, Fitri; Yusnita, Yusnita; Utomo, Setyo Dwi
JURNAL AGROTROPIKA Vol. 24 No. 1 (2025): Jurnal Agrotropika Vol 24 No 1, Mei 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i1.10286

Abstract

Indonesia is one of the largest cassava producing countries in the world with various types and advantages. Waxy clones have a high starch content and therefore they can be used as a food substitute for rice. Tissue culture through somatic embryogenesis is an effective technique for rapid and mass seedling propagation. This research aimed to determine the effect of the type and concentration of auxin 2,4-D or picloram and the addition of NAA on the formation of Waxy cassava clone somatic embryos. This research used a completely randomized design (CRD) which was arranged in a non-factorial. Auxin 2,4-D and picloram were used at four concentration levels: 0 mg.l-1, 8 mg.l-1, 10 mg.l-1, 12 mg.l-1, and additional NAA treatment of 6 mg.l-1 each. Results showed that MS + 6 mg.l-1 NAA treatment produced the highest callus weight. Meanwhile, the percentage of embryonic callus and number of embryo was higher in the treatment of MS + picloram 12 mg.l-1 and NAA 6 mg.l-1 (19,05 ± 5,82 %) and MS + 8 mg.l-1 2,4-D + 6 mg.l-1 NAA (11,11 ± 3,17 %) with 5,48 ± 3,56 and 4,93 ± 1,95 embryos, respectively. Thus, auxin picloram 12 mg.l-1 + NAA 6 mg.l-1 were the most effective in inducing the formation of somatic embryos on waxy cassava clone.
Optimalisasi Manajemen Komunikasi di TikTok untuk Meningkatkan Interaksi dan Retensi Pengguna Muda: Studi Kasus pada Save the Children Perianti K, Linda; Yusnita, Yusnita
Cerdika: Jurnal Ilmiah Indonesia Vol. 5 No. 7 (2025): Cerdika: Jurnal Ilmiah Indonesia
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/cerdika.v5i7.2650

Abstract

TikTok, sebagai platform media sosial berbasis video pendek, telah merevolusi cara generasi muda berinteraksi dan mengonsumsi konten. Dengan lebih dari 1 miliar pengguna aktif di seluruh dunia, TikTok menawarkan peluang unik bagi organisasi non-profit untuk menjangkau audiens muda dengan cara yang inovatif dan menarik. Save the Children, sebuah organisasi internasional yang berfokus pada kesejahteraan anak-anak, telah memanfaatkan TikTok untuk meningkatkan kesadaran akan isu-isu sosial serta melibatkan audiens muda dalam kampanye mereka. Penelitian ini bertujuan untuk mengidentifikasi dan menganalisis strategi komunikasi yang diterapkan oleh Save the Children di TikTok guna meningkatkan interaksi dan retensi pengguna muda. Melalui metode kualitatif deskriptif, penelitian ini menganalisis konten TikTok Save the Children, melakukan wawancara mendalam dengan pengelola akun, serta menyebarkan survei kepada pengguna muda. Hasil penelitian menunjukkan bahwa penggunaan konten kreatif berbasis storytelling, kolaborasi dengan influencer, dan pemanfaatan tren platform secara efektif mampu meningkatkan keterlibatan audiens muda. Konten yang mengedukasi sekaligus menghibur terbukti lebih efektif dalam menarik perhatian dan mempertahankan minat pengguna. Selain itu, kolaborasi dengan influencer yang memiliki basis pengikut yang relevan membantu memperluas jangkauan pesan organisasi. Penelitian ini memberikan rekomendasi praktis bagi organisasi non-profit lainnya dalam memanfaatkan TikTok sebagai alat komunikasi strategis. Dengan memahami cara berinteraksi dengan audiens muda melalui platform ini, organisasi dapat meningkatkan dampak sosial mereka dan memperluas jangkauan pesan-pesan penting yang ingin disampaikan. Kesimpulan dari penelitian ini menekankan pentingnya adaptasi terhadap perubahan tren media sosial dan kebutuhan audiens untuk mencapai keberhasilan dalam kampanye komunikasi.
PENINGKATAN KESADARAN MASYARAKAT TENTANG GAYA HIDUP SEHAT UNTUK MENCEGAH DIABETES MELLITUS Lestari, Tutik; Yusnita, Yusnita; Andiani, Andiani; Sumarto, Sabaria; Aldjokdja, Retno Kartika; Hasan, Risma Hi; Laego, Riyanti; Daud, Ruslia; Manteto, Tiara; Diman, Tiara; Aulia, Rifanda; Salim, Nurmai; Minggu, Musdalifa; Sahsun, Darmila; Langasa, Siti AIgina; Bada, Wahdi; M, Fitra Tunisyah; Fadilah, Fadilah
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 5 (2025): MAJU : Indonesian Journal of Community Empowerment, September 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i5.1750

Abstract

Diabetes mellitus is a non-communicable disease whose prevalence continues to increase globally, primarily due to unhealthy lifestyles. A healthy lifestyle, which includes a balanced diet, regular physical activity, stress management, and adequate sleep, plays a crucial role in preventing diabetes mellitus. This community service programme aims to increase public understanding of the importance of a healthy lifestyle as a preventative measure for diabetes mellitus. The activity, conducted in Tosa Village, targeted women from the Family Welfare Movement (PKK) through lectures, discussions, and Q&A sessions. The results showed an increase in the average pre- and post-test scores, from 5.39 to 12.79. It is hoped that this health education programme will help the community change unhealthy behaviors to adopt healthy ones in their daily lives to avoid diabetes mellitus and other diseases.
KELURAHAN BINAAN : PROGRAM PEMBERDAYAAN MASYARAKAT DI KELURAHAN TOSA Lestari, Tutik; Andiani, Andiani; Yusnita, Yusnita; Daud, Ruslia; Aulia, Rifanda; Aldjokdja, Retno Kartika; Sahsun, Darmila; Sumarto, Sabaria; Diman, Tiara; Langasa, Siti AIgina; Bada, Wahdi; Manteto, Tiara; Salim, Nurmai; M, Fitra Tunisyah; F, Fadila S. Hi.; Hasan, Risma Hi; Laego, Riyanti; Minggu, Musdalifa
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 5 (2025): MAJU : Indonesian Journal of Community Empowerment, September 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i5.1776

Abstract

In an effort to increase public awareness and involvement in the importance of environmental protection, the Faculty of Health Sciences, University of Muhammadiyah North Maluku (FIKes UMMU) initiated the Assisted Village program as a form of community service. This program aims to build synergy between the academic world and the community in creating a healthy, clean, and sustainable environment. Through a participatory approach, FIKes UMMU not only provides education on environmental health but also encourages the community to actively participate in concrete actions to maintain the cleanliness and sustainability of the environment around them. By making the village as a fostered partner, it is hoped that there will be collective behavioral changes that support community-based health development.
APLIKASI CAMPURAN AUKSIN NAA DAN IBA PADA PERTUMBUHAN DAN HASIL UBIKAYU Setiya Wati, Tri Agus; Yusnita, Yusnita; Asmara, Sandi; Karyanto, Agus; Widyastuti, Raden Ajeng Diana
JURNAL AGROTROPIKA Vol. 24 No. 2 (2025): Jurnal Agrotropika Vol 24 No 2, Oktober 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i2.10801

Abstract

The growth and production of cassava can be increased through various efforts, including the use of superior varieties, fertilization, the addition of organic materials, and the application of growth regulators (PGR). Naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) are PGRs that have been widely documented to stimulate root initiation and development in cuttings. The application of aauxin mixtures on cassava cuttings followed by fertilization and the addition of organic materials is expected to improve the growth and yield of cassava. The aim of this experiment is to study the effects of the application of an NAA and IBA mixture (1:1) and the type of fertilizer on the growth and yield of the Garuda variety of cassava. This research is designed using a clustered randomized block method with three replications. The design used is factorial 2x3, consisting of two main treatment factors. The first factor is the combination of growth regulators NAA and IBA in a 1:1 ratio at three concentration levels, namely 0 ppm, 500 ppm, and 1,000 ppm. The second factor includes the type of fertilizer used, which is a single inorganic fertilizer and a combination of inorganic fertilizer with cow manure. Each experimental unit in this study consists of 10 cassava cuttings planted in an orderly manner with a planting distance of 1 meter x 1 meter between plants. The results of the experiment showed that the application of inorganic fertilizer produced plant growth and the number of productive roots equivalent to the use of inorganic fertilizer + cow manure. The application of a mixture of auxins NAA and IBA (1:1) at concentrations of 500 ppm and 1,000 ppm significantly increased the growth of shoots, the number and weight of productive roots of the Garuda variety cassava, but both treatments resulted in cassava growth and yield that were not different. Key words: Cassava, cow manure, growth, IBA, NAA, yield
RESPON PERTUMBUHAN DAN HASIL PADI VARIETAS IR NUTRIZINC TERHADAP APLIKASI BIOCHAR DAN BENZILADENIN Rafika, Eni; Yusnita, Yusnita; Karyanto, Agus; Hapsoro, Dwi; Pramono, Eko
JURNAL AGROTROPIKA Vol. 24 No. 2 (2025): Jurnal Agrotropika Vol 24 No 2, Oktober 2025
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/ja.v24i2.10895

Abstract

Rice growth and yield can be increased through various efforts, including the use of "high-yielding varieties, fertilization, soil conditioners with application of plant growth regulators (PGRs). Soil conditioners namely as biochar are carbon products that can enhance plant productivity through enhancing the physical, chemical, and biological characteristics of the soil, while cytokinine benzyladenine (BA) is a PGR that has been widely documented to stimulate cell growth, cell division, and differentiation in rice plants. The purpose of this study was to determine the effect of biochar and benzyladenine (BA) on the growth and yield of the IR Nutrizinc rice variety. Data collection was carried out in October 2024 - February 2025 in Kampung Payung Rejo, Pubian District, Central Lampung Regency. The treatments were arranged factorially (2x3) using a completely randomized design with 3 replications. Factor I was without biochar and given 20 tons/ha of biochar. The second factor was 3 benzladenin (BA) concentrations, namely 0 ppm, 50 ppm, and 100 ppm applied by spraying on the surface of the plant at 20, 30, and 40-days after transplanting, while the biochar application was implemented 2 weeks before transplanting. Observed variables included plant height, maximum tillers per clump, count of productive tillers, length of panicles, and grains per panicle, percentage of empty grains per panicle, dry harvested grain yield, dry harvest grain yield, 1000 grain weight and dry weight of the crownThe Bartlett test was employed to assess the homogeneity of variance among treatments then analysis of variance was carried outIf a notable difference existed between treatments, the BNT test was carried out at the 5% significance level. The findings indicated that applying 20 tons/ha of biochar enhanced the yield components in terms of dry harvested grain, dry milled grain, and dry shoot weight The application of 50 ppm BA was able to increase the growth and yield of grain compared to without benzyladenine (BA). The combination of biochar and BA enhanced the growth and yield of grain in terms of the number of grains per panicle, dry harvested grain, and dry milled grain. The use of 20 tons/ha biochar combined with 50 ppm BA enhanced the yield of the IR Nutrizinc
Correlation of Human Telomerase Reverse Transcriptase Promoter (hTERT) Gene Methylation and Ageing Nihayah, Silviatun; Wening Sari; Yusnita, Yusnita; Intan Razari; Kinasih Prayuni; Utomo, Ahmad Rusdan Handoyo
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22038

Abstract

Promoter methylation of the hTERT gene in blood DNA has been proposed to be an epigenetic molecular clock because it is negatively correlated with ageing. Saliva is an alternative source of DNA because it contains buccal cells. Currently, the correlation of hTERT promoter methylation and ageing using saliva is not known. This study aimed to determine: first, the correlation between hTERT promoter methylation and ageing in saliva, as a source of non-invasive DNA sampling, instead of blood was determined. Second, the influence of sex on the methylation of the hTERT promoter in ageing was evaluated. A cross-sectional study design was used, and 119 subjects were recruited, consisting of 25 children (1-5 y.o), 42 teens (17-19 y.o), 16 adults (20-50 y.o), and 36 elderly (60 to 84 y.o). Promoter methylation of the hTERT of extracted DNA was determined using the MSRE (methyl-specific restriction enzyme) method. The relationship between age and the percentage of hTERT methylation was assessed using the Pearson test. The percentage of hTERT methylation in saliva DNA was negatively correlated with age r= -0.4305 (p-value <0.05). Negative correlation was also found in men (r= -0.376) and women (r=-0.43). Negative correlation between hTERT and ageing has been confirmed in saliva as a noninvasive sampling method. The benefit of this research in ethical and social considerations may encourage a greater participation in ageing research, particularly in underprivileged communities, thus democratising access to scientific advancements in longevity studies.
Faktor-Faktor Yang Berhubungan Dengan Penggunaan Kontrasepsi Implant Di Puskesmas Garuda Kota Pekanbaru Tahun 2025 Yusnita, Yusnita; Husanah, Een; Nurhapipa, Nurhapipa
Journal of Innovative and Creativity Vol. 5 No. 3 (2025)
Publisher : Fakultas Ilmu Pendidikan Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Implant merupakan pilihan metode kontrasepsi jangka panjang, termasuk pilihan metode kontrasepsi hormonal, implant dapat segera dipasangkan pada ibu sesaat setelah bersalin atau sebelum meninggakan fasilitas dan implant tidak menggangu produksi ASI sehingga dapat digunakan bagi ibu yang akan menyusui bayinya. Puskesmas Garuda Kota Pekanbaru termasuk salah satu puskesmas yang penggunaan kontrasepsi implantnya masih rendah yaitu sebesar 2,6%. Hal ini menunjukkan bahwa target masih belum tercapai dan cakupan peserta KB Implant masih rendah. Penelitian ini bertujuan untuk mengetahui Faktor-Faktor Yang Berhubungan Dengan Penggunaan Kontrasepsi Imlant di Puskesmas Garuda Kota Pekanbaru. Adapun penelitian ini dilakukan dengan memberikan kuisioner, penelitian menggunakan metode kuantitatif analitik korelasi dengan pendekatan cross sectional dengan sampel berjumlah 96 orang. Sampel penelitian ini adalah Pasangan Usia Subur (PUS) yang merupakan peserta kb aktif. Teknik pengambilan sampel dalam penelitian ini adalah Accidental Sampling. Hasil penelitian didapatkan dari 96 responden, proporsi ibu yang menggunakan kontrasepsi implant sebesar 16,7% dan yang tidak menggunakan kontrsepsi implant sebesar 83,3%. Kesimpulan dalam penelitian ini adalah bahwa tidak ada hubungan antara variable umur (p-value=0,090), variabel pendidikan (p-value=0,703), paritas (p-value=0,246) dan ada hubungan antara variable pengetahuan (p-value=0,001) terhadap penggunaan kontrasepsi implant. Diharapkan bagi Puskesmas Garuda Kota Pekanbaru dapat meningkatkan program KB di wilayah kerja puskesmas Garuda Kota Pekanbaru.
Co-Authors , Agustiansyah Abdul Latif Adrian Tawai Agus Karyanto Akhir, Chorunnisa Yaumal Aldjokdja, Retno Kartika Aldri Frinaldi Amos Amos Andiani Andiani, Andiani Andri Yulianto, Andri Armiyanti, Armiyanti Aulia, Rifanda Bada, Wahdi Bakara, Tiarlince Batubara, Lilian Chaerunnimah . Christiawati, Devi Cipta Ginting, Cipta Daud, Ruslia Desi Kurniawati Desi Maulida Dewi, Eva Ratna Dhini Ayu Triningsih Diman, Tiara Dina Nurdinawati, Dina Doni Stiadi DWI HAPSORO Ekawati Danial Eko Pramono Elianora - Erlangga, Wahyu F, Fadila S. Hi. Fadilah Fadilah, Fadilah Farida Hanum Fathul Jannah, Fathul Fitri Yelli Fitriani Fitriani Ginta Siahaan Halika, La Ode Herman Hamid, Syukri Al Hamiranti, Rahmadyah Handayani, Yivista Harahap , M Syahnan Haristian Hergandi Haryanto Haryanto Hasan Fauzi, Hasan Hasan, Risma Hi Hatta, Moh Herda, Wahyuni Hi. A. Djafar, Monisa Husanah, Een Intan Razari Isni Hijriana Istiqomah, Dyah Pramesti Ivayani, Ivayani Joko Joko Joleha, Joleha Kadir Kadir Kalsum, Ummy Kasmiyeti, Kasmiyeti Keke, Ahmat Kholis Ernawati Kinasih Prayuni Kurniawaty Fitri La Iba, La Laego, Riyanti Langasa, Siti Aigina Listika, Listika Listuti Hariani M, Fitra Tunisyah M, Zasari M.Imamuddin, M.Imamuddin Maera Zasari Manteto, Tiara Mellani Yuliastina MERLEY MISRIANI Minggu, Musdalifa Muhammad Saleh Mukherji, Rani Musawir, La Ode Abdul Neni Hermita Nihayah, Silviatun Ningsih, Winda M Noprida, Dayana Nordiansyah, Muhammad Novalia Novalia Novi Novi Nugroho, Rizki Bagus Nurhapipa, Nurhapipa O, Saputri Perarih, Sada Perianti K, Linda Prabowo, Sugma Agung Prayogi, Adi Noor Purwandari, Elce Raden Ajeng Diana Widyastuti Rafika, Eni Rahmatia, Annisa Ramadiana, Sri Rani, Siti Maha Ratu Ayu Dewi Sartika Razi, T. Khairol Resika, Dewi Ridwan Rifqaatusa'adah, Rifqaatusa'adah Rinanda, Della Okta Rizki, Fitria Rosmila Tuharea, Rosmila Rosnaini Daga Sahsun, Darmila Salim, Nurmai Sandi Asmara Septiasari, Yeti Setianingrum, Kusuma Ayu Setiya Wati, Tri Agus Setyo Dwi Utomo Shalihah, Mar'atun Sigit Santoso Siti A Pujonarti Situmorang, Tumpal P. Sofiati, Vivi Subakti, Agung Subekti, Reni Tri Sudu, La Sugito, Wahyu Sukmawati Sukmawati Sultani Sultani Sumarto, Sabaria Suri, Rindang Andam Suskandini Ratih Dirmawati Syahputra, Fadli Tamara, Fadhila Tri Dewi Andalasari Tunru, Insan Sosiawan A Tutik Lestari Utami, Melsya Halim Utami, Nurul Amalia Utomo, Ahmad Rusdan Handoyo Wahyudiyono, Wahyudiyono Wening Sari Widianti, Dian Widianti, Dini Wijayanti, Erlina Wirawan, Fadila Wiwit Sariasih Wulandari, Ririn Arminsih Wulansari, Rifda Yulidar Yulidar Yurnalis Yurnalis Zakaria Zakaria Zulheri, Zulheri