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Journal : Jurnal Veteriner

Aktivitas Ekstrak Meniran (Phyllanthus niruri linn) Sebagai Immunostimulator pada Ayam yang Divaksin Penyakit Tetelo Jola Rahmahani; Rahaju Ernawati; Didik Handijatno
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.715 KB) | DOI: 10.19087/jveteriner.2021.22.1.125

Abstract

Newcastle Disease or tetelo is one of main problem in poultry Industry in Indonesia. Prevention such as biosecurity control and routin vaccination program have been conducted to overcome this problem, but they have not given any great impact. Phyllanthus Niruri L. or meniran is well known as immunostimulatory. This research was aimed to reveal effect of Phyllanthus Niruri L. extract on chicken vaccinated with live vaccine LaSota. Administration of Phyllanthus Niruri L. extract was conducted on three different time which were 7 days before vaccination, 1 days after vaccination, and 3 days before and after vaccination. The amount of Phyllanthus Niruri L. extract administered were 2 ml, 2.5 ml, and 3 ml orally. Data of antibody titre were collected for 4 weeks after the treatment. It was obtained by measuring the antibody through Haemagglutination Inhibition test each week. According to the result Phyllanthus Niruri L. extract could increase the amount of antibody titre against Newcastle Disease. The amount of Phyllanthus Niruri L. extract given that capable to induced maximum of antibody titre was administered 1 days after the vaccination with amount 2.5 ml. It is suggested that Phyllanthus Niruri L. extract should be administered post vaccination to boost antibody synthesis.
Aktivitas Antimikrob Cuka Apel terhadap Multidrug Resistance Staphylococcus aureus yang Diisolasi dari Luka Infeksi Anjing di Surabaya Elisa Herina Dimariwu; Wiwiek Tyasningsih; Jola Rahmahani; Rahaju Ernawati; Mustofa Helmi Effendi; Didik Handijatno
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.917 KB)

Abstract

Staphylococcus aureus is one of the normal flora that can cause infection in injured skin. Resistance to antibiotics has an impact on the difficulty of therapeutic treatment so that other alternatives are needed. The purpose of this study was to observe the effectiveness of apple vinegar as an antimicrobial against Multidrug Resistant Staphylococcus aureus isolated from infection wounds in dogs in Surabaya. The methods in this study were the isolation of bacteria from 30 samples of dog festering wounds on Manitol Salt Agar (MSA) media and identification through macroscopic, microscopic, catalase tests, coagulase tests, hemolysis tests on Blood Agar media, and Voges–Proskauer (VP) tests. Bacteria that have included the S. aureus criteria were followed by sensitivity tests to the antibiotics Amoxycillin, Ampicillin, Gentamicin, Chloramphenichol, and Ciprofloxacin. Apple vinegar activity test was carried out using disk diffusion method against Multidrug Resistant Staphylococcus aureus. The results showed that of the seven S. aureus isolates, there were two isolates belonging to the Multidrug Resistant S. aureus. The results of the apple vinegar activity test showed the presence of antimicrobial activity shown by the formation of a clear zone around the paper disk with an average diameter of 24.06 mm at a concentration of 90%. The conclusion shows that apple vinegar has antimicrobial activity against Multidrug Resistant S. aureus which is isolated from dog festering wounds in Surabaya.
Analisis Filogenetik Gen Hemaglutinin dan Neuraminidase Avian Influenza H9N2 Asal Ayam Petelur di Jawa Timur (PHYLOGENETIC ANALYSIS OF HAEMMAGLUTININ AND NEURAMINIDASE GENES OF AVIAN INFLUENZA H9N2 FROM LAYER INI EAST JAVA) Prestalia Dwi Rachmawati; Tatang Santanu Adikara; Hani Plumeriastuti; Rahaju Ernawati; Jola Rahmahani; Didik Handijatno; Christian Marco Hadi Nugroho
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (326.985 KB)

Abstract

Avian influenza virus (AIV) subtype H9N2 is one of the infectious agents that threatens laying poultry farms, because it has an impact on drastically reducing production in the population. The aim of this study was to isolate and analyze phylogenetically the partial gene encoding the surface proteins of the AIV subtype H9N2 from laying hens in East Java. A total of 30 suspected AIV subtypes of H9N2 were taken from laying hens which had decreased production by up to 70% in three sub districts each in Kediri, Blitar and Tulung Agung regency, in East Java Province. The virus was isolated in embryonated chicken eggs and then followed by a Hemagglutination (HA) test. Detection of the presence of H9 and N2 genes was carried out through Reverse Transcriptase polymerase Chain Reaction (RT-PCR) and continued with partial gene sequencing of the two surface proteins. Data analysis was processed using BioEdit version 7.2.5 and Mega 7.0. The results show that only one sample, namely code B2.2 from Blitar regency, is an AIV subtype of H9N2. The virus in this study belong to clade h9.4.2.5 of the AIV subtype H9N2. The conclusion of this study is that the VAI subtype H9N2 was successfully isolated from laying hens in East Java and successfully identified phylogenetically.
Co-Authors A'la, Rofiqul A. T. Soelih Estoepangestie Adikara, Tatang Santanu Agustono, Bodhi Akbar, Daniswara Irwanza Akhmad Taufiq Mukti Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Ali Agus Amal, Mohammad Noor Azmai Amelia, Ezanti Nur Annas Salleh Aprinda Ratna Lovela Ardhanella, Sellianova Arif Nur Muhammad Ansori Asri Rizky Asri Rizky Astria Novitasari Nidom Astuti, Elita Sriwidya Aswin, Ahmad Azahro, Aisyah Aziz, Githa Nurma Balqis Afifah Bambang Sektiari Lukiswanto Baroroh, Diana Nova Boedi Setiawan BUDI UTOMO Christian Marco Hadi Nugroho Chusniati, Sri Daruti Dinda Nindarwi Dewi, Nina Nurmalia Dhani Prakoso Didik Handijatno Didik Handijatno Dita Wisudyawati Diyantoro Diyantoro, Diyantoro Elisa Herina Dimariwu Erma Safitri Ernawati, Rahaju Ernawati, Rahayu Fachriza Tri Aprilia Fachrur Rozi Fadlilah, Shinta Levea Ni'matul Faisal Fikri Fedik Abdul Rantam Fidyah Fitrawati, Fidyah Gazali Salim Haeriah Hani Plumeriastuti Harijani, Nenny Helen Susilowati, Helen Herry Agoes Hermadi Hutahaean, Ryan David Pandapotan IMAM MUSTOFA Imam Mustofa Indah Rahmawati Ine Karni Ira Sari Yudaniayanti Irfani, Dyah Ismudiono Iwan Sahrial Hamid Kadek Rachmawati Kadek Rachmawati Karo-karo, Desniwaty Khairullah, Aswin Rafif Khanand, Nadeem Kirana, Maha Kuncoro Puguh Santoso Kuncorojakti, Suryo Kuncorojati, Suryo Kurniasari, Pitaloka Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lucia Tri Suwanti, Lucia Tri Meles, Dewa Ketut Mohammad Noor Amal Azmai Mohammad Sofi’ul Anam Muchammad Yunus Muhammad Arief Muhammad Khaliim Jati Kusala Muhammad, Abdul Latif Mustofa Helmi Effendi Nidom, Chairul Anwar Nor Laela Ramadhaniyah Nurwartanti, Maya Nusdianto Triakoso Pasang, Gabriel Sampe Pinayungan, Probo Probo Warih Tatag Pradhita, Dewanggi Kristi Sasmi Praja, Ratih Novita Prestalia Dwi Rachmawati Putih, Nurvita Putri Desi Wulan Sari Putri, Diana Novita Rahaju Ernawati Rahaju Ernawati Rahmawati, Anlisia Rahmawati, Kadek Rahmi Sugihartuti Ramandinianto, Sancaka Chasyer Ratna Damayanti Reviany Vibrianita Nidom Rimayanti Rimayanti Rosyta, Pegy Rozi Sabdoningrum, Emy Koestanti Salleh, Annas Santanumurti, Muhammad Browijoyo Sari, Putri Desi Wulan Setyarina Indrasari Siti Komariyah Soeharsono Soeharsono Soeharsono Supriyadi Supriyadi Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Suwarno Tacharina, Martia Rani Tiara Wandira Hariyanto Tita Damayanti Lestari Tyasningsih, Wiwiek Tyasningsih, Wiwiek Venri Novryantoro Vensko Zico Dandi Wahyu Pratama Wibawati, Prima Ayu Widjaja, Nanik Sianita Wijaya, Andi Yasmin Wijaya, Nanik Sianita Wiwiek Tyasningsih Wiwiek Tyasningsih Wiwik Misaco Yuniarti Woro Hastuti Setyantini Wudhu, R Zaksara Fero Ali Mutaqin Wulandari, Innah Yudhana, Aditya Yulianna Puspitasari Yunita, Maya Nurwartanti Yunus , Muchammad Yunus, Muchammad Zayyin Dinana Zulfanisa, Rizky Asrin Zulianti, Brillia