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All Journal Jurnal Akuakultur Indonesia ACTA VETERINARIA INDONESIANA Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) BERITA BIOLOGI Omni-Akuatika LIMNOTEK - Perairan Darat Tropis di Indonesia Jurnal Perikanan Universitas Gadjah Mada Jurnal Intek Akuakultur Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Abdi Insani Jurnal Pusat Inovasi Masyarakat Journal of Fisheries & Marine

Agus Oman Sudrajat

Department Of Aquaculture, Faculty Of Fishery And Marine Sciences, Bogor Agricultural University, Darmaga, Bogor 16680, Indonesia

Author-ID : 2472806

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Maturational induction of grass carp, Ctenopharyngodon idella (Valenciennes, 1844) using hormone and Indigofera zollingeriana feed Dwi Mulyasih; Agus Oman Sudrajat; Luki Abdullah
Jurnal Iktiologi Indonesia Vol 16 No 1 (2016): February 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i1.49

Pregnant mare serum gonadotropin (PMSG) premix hormone and anti dopamine were used to accelerate maturation. Indigofera zollingeriana a legume plant that has high nutrition content and carotenoids whose has function in oocyte development. The aim of this research was to accelerate the maturation of grass carp (Ctenopharyngodon idella) using premix hormone and to replace the commercial feed with indigofera. The research was conducted in couple randomize design (CRD), using five treatments which were A (NaCl 0.5 ml kg-1 body weight + commercial feed), B (NaCl 0.5 ml kg-1 body weight + indigofera feed), C (premix hormone 0.5 ml kg-1 body weight + commercial feed), D (premix hormone 0.5 ml kg-1 body weight + indigofera feed) and E (premix hormone 0.5 ml kg-1 body weight in indigofera feed) and five fishes as individual replication. The result showed that 17P- estradiol concentration reached the highest level at 4-week on C treatment 1194.8 pgmL-1, while in E treatment 17P-estradiol concentrations was higher than the other treatments at 8-week. At the end of research period, the highest gonadosomatic index (GSI) was performed by E treatment. Addition of premix hormone on feed could increase the gonadal maturation up to perinucleus phase, while in control was still immature. These results indicated that premix hormone could induce gonadal maturity, indigofera could replace commercial feed as broodstock diet, and gonadal maturation through hormonal induction could be perform by giving more competitive feed. Abstrak Premiks hormon pregnant mare serum gonadotropin (PMSG) dan anti dopamin dapat mempercepat pematangan gonad pada ikan. Indigofera zollingeriana merupakan tumbuhan leguminosa yang memiliki nutrisi tinggi dan mengandung ka-rotenoid yang berfungsi untuk perkembangan oosit. Tujuan penelitian ini untuk mempercepat pematangan gonad meng-gunakan premiks hormon serta menggantikan pakan komersial dengan pakan indigofera pada ikan koan (Ctenopha-ryngodon idella). Penelitian menggunakan rancangan acak lengkap dengan lima perlakuan yaitu A (NaCl fisiologis 0,5 ml kg-1 bobot tubuh + pakan komersial), B (NaCl fisiologis 0,5 ml kg-1 bobot tubuh + pakan indigofera), C (premiks hormon 0,5 ml kg-1 bobot tubuh + pakan komersial), D (premiks hormon 0,5 ml kg-1 bobot tubuh + pakan indigofera), E (premiks hormon 0,5 ml kg-1 bobot tubuh dalam pakan indigofera) dan lima kali ulangan individu. Hasil penelitian me-nunjukkan bahwa estradiol-17p mencapai puncak pada minggu keempat pada perlakuan C yaitu sebesar 1194,8 pg ml-1 sedangkan pada perlakuan E konsentrasi estradiol-17p lebih tinggi daripada perlakuan lain pada minggu kedelapan. Pada akhir penelitian indeks kematangan gonad tertinggi diperoleh pada perlakuan E. Penambahan premiks hormon pada pakan dapat meningkatkan kematangan gonad hingga mencapai fase perinukleus sedangkan pada kontrol tidak berkem-bang. Hasil ini menunjukkan bahwa premiks hormon dapat menginduksi kematangan gonad, pakan indigofera dapat menggantikan pakan komersial sebagai pakan induk, dan induksi pematangan gonad secara hormonal dapat dilakukan dengan pemberian pakan yang lebih kompetitif.

Sex reversal mechanism in Nile tilapia Oreochromis niloticus (Linnaeus, 1758) by manipulation of aromatase gene expression Upmal Deswira; Agus Oman Sudrajat; Dinar Tri Soelistyowati
Jurnal Iktiologi Indonesia Vol 16 No 1 (2016): February 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i1.50

Sex reversal is a sex conversion technique to be male or female. This research was conducted to evaluate the mechanism of sex reversal in tilapia on the use of aromatase inhibitors (AI), honey and pesticide. The eye spot embryo of Nile tilapia was immersed into those solutions and examined the expression of aromatase gene and sex ratio. The sex reversal was carried out by immersion of eye spot embryo for 24 hours using 20 mg L-1 AI (imidazole), 10 ml L-1 honey (Perhutani, longan flower), and 1 ^L L-1 pesticide (Decis: deltamethrin 25 g L-1). Aromatase gene expression was analyzed on 1st and 8th day after treatment. Larvae were reared until 75 days-old. The level of gene expression of ovarian type aromatase in imidazole, honey, and pesticide treatment on 1st day after treatment were lower than control. While, on 8th day the lowest level of gene expression was on imidazole treatment and the highest was on pesticide treatment. The results indicated that masculinization occurred in imidazole treatment and feminization occurred in pesticide treatment which showed by male percentage that significantly different from the control (p<0.05). The male percentage of control, imidazole, honey, and pesticide were 68.32%, 80.77%, 70.93% and 50.45%, respectively. Honey treatment was not significantly different from control. In conclusion, sex reversal was influenced by modulation of gene expression of ovarian type aromatase, the increasing of gene expression of ovarian type aromatase caused feminization, and otherwise the decreasing of gene expression caused masculinization in early development stage of nile tilapia. Abstrak Alih kelamin merupakan suatu teknik pengalihan kelamin menjadi jantan atau betina. Penelitian ini bertujuan mengeva-luasi mekanisme alih kelamin ikan nila pada penggunaan penghambat aromatase, madu, dan pestisida. Ikan nila fase bintik mata direndam dalam tiga larutan tersebut dan diamati ekspresi gen aromatase dan nisbah kelaminnya. Peren-daman dilakukan selama 24 jam menggunakan penghambat aromatase (imidazole) 20 mg L-1, madu (Perhutani, bunga kelengkeng) 10 ml L-1, dan pestisida (Decis: deltametrin 25 g L"1) 1 ^L L-1. Kemudian dilakukan analisis ekspresi gen aromatase pada hari ke-1 dan ke-8 setelah perlakuan. Selanjutnya larva dipelihara sampai berumur 75 hari. Tingkat ekspresi gen aromatase tipe ovari perlakuan imidazole, madu, dan pestisida pada hari ke-1 setelah perlakuan lebih rendah dibandingkan kontrol. Pada hari ke-8 tingkat ekspresi gen paling rendah pada perlakuan imidazole sedangkan yang paling tinggi pada perlakuan pestisida. Hasil ini menunjukkan bahwa terjadi proses maskulinisasi pada perlakuan imidazole dan feminisasi pada perlakuan pestisida yang diketahui dari persentase jantan berbeda nyata dibandingkan dengan control (p<0,05). Nilai persentase jantan pada perlakuan kontrol, imidazole, madu, dan pestisida secara berurutan yaitu 68,32%, 80,77%, dan 50,45%. Perlakuan madu tidak berbeda nyata dengan kontrol. Disimpulkan bahwa alih kelamin dipengaruhi oleh perubahan ekspresi gen aromatase tipe ovari, peningkatan ekspresi gen aromatase tipe ovari menyebabkan feminisasi, sebaliknya penurunan ekspresi gen menyebabkan maskulinisasi di perkembangan awal larva ikan nila.

Hormonally induced gonadal maturation in eels, Anguilla bicolor bicolor McClelland 1984 with the use of Pregnant Mare Serum Gonadotropin, anti dopamin, and recombinant Growth Hormone Hadra Fi Ahlina; Agus Oman Sudrajat; Tatag Budiardi; Ridwan Affandi
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.57

The aim of this study was to evaluate the effect of Pregnant mare serum gonadotropin (PMSG), Anti-Dopamine (AD) and Recombinant growth hormone (RGH) through the injection technique on gonadal development of eel (Anguilla bicolor bicolor). The experimental was arranged in completely randomized design with five treatments of hormone combination, namely P10A combination hormone (10 IU PmsG + 0.1 mgL-1 AD), P10B (10 IU PMSG + 0.1 mgL-1 AD + 10 ug RGH), P20A (20 IU PMSG + 0.1 mgL-1 AD), P20B (20 IU PMSG + 0.1 mgL-1 AD + 10 ug RGH), and PK (negative control). In each treatment, a total of 20 fish individuals used as replicates and the hormonal induction was conducted every week for eight weeks of the rearing period. Specific growth rate (SGR), hepatosomatic index (HSI), go-nadosomatic index (GSI) and an index of the eye (IM) were observed. The results showed that the P20A treatment increased spermatogenesis and the value of GSI (2.291±0.278%) at 4-6 weeks after injection. This result was higher than P20B (2.134±0.265%), P10B (2.065±0.201%), P10A (2.037±0.105%), and PK (1.937±0.050%). The value of HSI on the P20a treatment (1.188±0.091 %) was higher than other treatments. The highest value of SGR (0.514±0.062%) was found in the P20B, whereas the lowest value (0.052±0.027%) was found in the PK. Thus, the combination of pMsG, AD and RGH hormones can stimulate the development of testicular of eel with body weight 140 to150 g, and stimulate the growth of 0.514 % during the six-weeks rearing period. Abstrak Penelitian ini bertujuan mengevaluasi penggunaan hormon Pregnant Mare Serum Gonadotropin (PMSG), Anti Dopamin (AD) dan Recombinant Growth Hormone (rGH) melalui teknik penyuntikan terhadap pematangan gonad ikan sidat (Anguilla bicolor bicolor). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan kombinasi hormon yaitu 10 IU PMSG + 0,1 mgL-1 AD (P10A), 10 IU PMSG + 0,1 mgL-1 AD + 10 ^g rGH (P10B), 20 IU PMSG + 0,1 mgL-1 AD (P20A), 20 IU PMSG + 0,1 mgL-1 AD + 10 ^g rGH (P20B), dan PK (kontrol). Pada setiap perlakuan, sebanyak 20 ekor ikan digunakan sebagai ulangan individu dan sampling dilakukan setiap minggu selama delapan minggu masa pemeliharaan. Parameter yang diamati meliputi nilai laju pertumbuhan spesifik (LPS), indeks he-patosomatik (IHS), indeks gonadosomatik (IGS) dan indeks mata (IM). Hasil penelitian menunjukkan bahwa perlakuan P20A menyebabkan perkembangan spermatogenesis dan meningkatkan nilai IGS tertinggi (2,291±0,278%) pada minggu ke empat hingga ke enam setelah penyuntikan dibandingkan perlakuan P20B (2,134±0,265%), P10B (2,065± 0,201%), P10A (2,037±0,105%) dan PK (1,937±0,050%). Nilai IHS pada perlakuan P20A (1,188±0,091%) lebih tinggi dibandingkan dengan perlakuan lainnya. Nilai LPS tertinggi ditemukan pada perlakuan P20B (0,514±0,062 %) dan te-rendah pada perlakuan Kontrol (0,052±0,027%). Nilai IM juga meningkat pada perlakuan P20B (10,599±2,372) seiring dengan bertambahnya bobot tubuh dan terendah pada perlakuan PK (7,189±0,217). Kombinasi hormon PMSG, AD dan rGH dapat merangsang perkembangan testis ikan sidat ukuran 140-150 g serta memacu pertumbuhan 0,514 %.

Ekspresi gen aromatase pada pengarahan diferensiasi kelamin ikan nila (Oreochromis niloticus Linnaeus 1758) menggunakan madu Eny Heriyati; Alimuddin Alimuddin; Harton Arfah; Agus Oman Sudrajat
Jurnal Iktiologi Indonesia Vol 15 No 1 (2015): Februari 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i1.74

In tilapia aquaculture, all male populations are preferred because they achieve higher growth rates and prevent uncontrolled reproduction. Sex reversal techniques are largely used for the control of sex in fish farming and in fundamental studies on sex determinism mechanisms. The study was conducted to determine the effect of immersion Nile tilapia larvae in water containing different honey source on male percentage and aromatase gene expression. In experiment I, a total of 30 tilapia larvae at 12 days post hatch were immersed in water containing honey derived from the forest, cultured and mangrove bees, at a dose of 10 ml L-1 for 10 hours. Fish were maintained in the same condition for two months. The results showed that percentage of male fish was similar among honey treatments (p>0.05), and they were significantly different with the control (p<0.05). In experiment II, fish were immersed in two bioactive compounds of honey, namely chrysin and potassium solution in a dose of 20 mg L-1 and 0.026 g L-1, respectively, to verify the bioactive affects sex differentiation. Aroma-g expression was analyzed by RT-PCR method. Tissue was collected at 1, 6, 12, 24 and 48 hours after immersion, and 2-month-old fish. Size fragment DNA aroma-g of female 200 bp. Chrysin and potassium immersion increased male percentage (p<0.1), this indicated that both materials were involved in Nile tilapia sex differentiation. RT-PCR analysis showed that honey, chrysin and potassium down-regulated aroma-g expression at 12 hours post immersion. Thus, honey can be used for sex reverse of Nile tilapia, and the mechanism is most likely as aromatase inhibitors. Abstrak Budi daya ikan nila dengan populasi jantan semua (monoseks) lebih memberikan keuntungan karena laju pertumbuhan-nya lebih cepat dan dapat mencegah pemijahan liar.Teknik pengarahan diferensiasi kelamin(sex reversal) digunakan untuk mengarahkan pembentukan jenis kelamin pada budi daya ikan.Penelitian ini dilakukan untuk mengevaluasi pe-ngaruh perendaman larva ikan nila menggunakan tiga sumber madu berbeda terhadap persentase ikan jantan dan ekspresi gen aromatase. Pada percobaan satu, 30 larva ikan nila berumur 12 hari setelah menetas direndam menggunakan madu hutan, madu ternak dan madu bakau, dengan dosis 10 ml L-1 air selama 10 jam. Ikan dipelihara dalam kondisi yang sama selama dua bulan. Hasil penelitian menunjukkan bahwa persentase ikan jantan tidak berbeda nyata antar perlakuan madu (p>0,05), tetapi semuanya berbeda nyata dengan kontrol (p<0,05). Pada percobaan kedua, larva ikan nila direndam dalam air mengandung dua bahan bioaktif madu, yakni chrysin dan kalium dengan dosis masing-masing 20 mg L-1 dan 0,026 g L-1. Ekspresi gen aromatase tipe gonad (aroma-g) dan tipe otak (aroma-o) dianalisis menggunakan metode RT-PCR. Sampel jaringan diambil pada waktu 1, 6, 12, 24, dan 48 jam pascaperlakuan madu, chrysin, dan kalium, serta setelah ikan berumur dua bulan.Ukuran fragmen DNA aromatase pada gonad betina sekitar 200 bp. Perendaman chrysin dan kalium meningkatkan persentase ikan jantan (p<0,1). Analisis RT-PCR menunjukkan bahwa madu, chrysin, dan kalium dapat menekan ekspresi gen aroma-g pada jam ke-12 pascaperendaman. Dengan demikian dapat disimpulkan bahwa madu, chrysin dan kalium dapat digunakan untuk pengarahan diferensiasi ikan nila, dan mekanis-menya seperti penghambat aromatase.

Pemaskulinan belut (Monopterus albus Zuiew 1793) dengan induksi penghambat aromatase untuk penyediaan calon induk jantan Hafif Syahputra; Agus Oman Sudrajat; Dinar Tri Soelistyowati
Jurnal Iktiologi Indonesia Vol 14 No 2 (2014): Juni 2014
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v14i2.91

Aromatase is an enzyme that functions to change testosterone in the biosynthesis of estrogen. Inhibition of aromatase can stop the synthesis of estradiole in masculinization of fish. This study aimed to evaluate the effects of aromatase inhibitors by injection for masculinization of Asian swamp eel (Monopterus albus Zuiew 1793). The samples of swamp eel consisted of individuals with 24±2 cm length and 6-14 grams/individual weight. The treatments of aromatase inhibitor using imidazole were conducted by injection at doses of 0.001; 0.01; 0.1 mg kg-1 once time a week for four times and the gonad were observed using histology method. The results showed that the injection of imidazole 0.1 mg kg-1 week produced 40% males, while at the less doses were 50-85.72% intersexes, whereas in control was 100% females. The concentration of plasma testosterone inclined when the doses of imidazole increased. The highest concentration of testosterone reached to 1.8 ng mL-1 at dose of aromatase inhibitor 0.1 mg kg-1 or increased three times compared with the control. These results indicated that aromatase inhibitor suppressed the aromatase gene expression that leads the decline of estradiol and increased testosterone. Abstrak Aromatase adalah enzim yang berperan mengubah testosteron dalam proses biosintesis hormon estrogen. Penghambat-an aromatase dapat menghentikan pembentukan estradiol pada pemaskulinan ikan. Penelitian ini bertujuan untuk meng-evaluasi pengaruh pemberian hormon penghambat aromatase melalui penyuntikan untuk pemaskulinan belut (Monopterus albus Zuiew 1793). Belut yang digunakan berukuran 24±2 cm dengan berat 6-14 g diberi perlakuan penyuntikan hormon penghambat aromatase yaitu imidazole dengan dosis 0,001; 0,01; 0,1 mg kg-1 bobot tubuh sekali tiap minggu sebanyak empat kali dan diamati gonadnya setiap dua minggu secara histologi serta dibandingkan dengan kontrol. Hasil penelitian menunjukkan bahwa perlakuan hormon imidazole 0,1 mg kg-1 bobot tubuh menghasilkan 40% individu jantan pada minggu ke-6, sedangkan pada dosis yang lebih rendah menghasilkan 50-85,72% individu interseks, dan pada kontrol 100% betina. Konsentrasi testosteron plasma meningkat sejalan dengan peningkatan dosis imidazol. Konsentra-si testosteron plasma yang tertinggi mencapai 1,8 ng mL-1 pada perlakuan 0,1 mg kg-1 bobot tubuh atau meningkat tiga kali lipat dibandingkan dengan kontrol. Hasil ini menunjukkan bahwa hormon penghambat aromatase menyebabkan ekspresi gen aromatase tertekan sehingga konsentrasi estradiol plasma menurun dan konsentrasi testosteron meningkat.

Perubahan plasma darah dan kematangan gonad pada ikan betina Tor soro di kolam pemeliharaan [Changes of blood plasma and gonadal maturity on female Tor soro in pond] Hesti Wahyuningsih; Muhammad Zairin Jr; Agus Oman Sudrajat; Ligaya ITA Tumbelaka; Wasmen Manalu
Jurnal Iktiologi Indonesia Vol 12 No 1 (2012): Juni 2012
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v12i1.127

Tor soro is an endemic species of freshwater fish in North Sumatera. Year by year the population of Tor soro tend to decrease. Currently,Torsorohashadtechnologyofdomestication. In order to support the success of breeding of Tor soro, information on the gonad development is urgently needed. Oocyte diameter and biochemistry of blood plasma of eight young females was carried out every month during a year (April 2009-March 2010). The estradiol-17p concentration was high received in July (0.9±0.8 ng ml'1), then decreased drastically in August (0.20±0.16 ng ml'1) and again increased until achieving the highest concentration in March.The highest of the estradiol-17p concentration correspond the peak of vitellogenesis towards the maturation. Biochemistry of blood plasma low in June as follow protein total 3.9±0.359 g dl-1; cholesterol 0.13±0.014 g dl-1; triglyceride 0.1±0.021 g dl-1 occurred at the time of the maximum size oocyte development. Concentration of low glucose existed in September (0.04±0.019 g dl-1) when the fish ovulated, this condition increased gradually up to optimal in February (0.12±0.003 g dl-1).Meanwhile optimal ovarian maturity occurred in June and September. AbstrakTor soro merupakan ikan endemik di Sumatera Utara dengan populasinya yang kian menurun. Saat ini, Tor soro telah mengalami teknologi domestikasi dan mampu dilakukan perbanyakan ikan pada kolam budidaya sejak tahun 2000. Informasi tentang reproduksi ikan ini masih sangat sedikit sehingga perlu adanya kajian tentang perkembangan gonad sebagai data awal pengembangan budi daya. Dalam studi ini digunakan delapan ekor betina muda. Pengukuran diameter oosit dan parameter biokimia plasma darah dilakukan sebulan sekali selama setahun (April 2009-Maret 2010). Konsen-trasi estradiol-17p yang tinggi diperoleh pada bulan Juli 2009 (0,9±0,8 ng ml-1), kemudian menurun drastis pada bulan Agustus 2009 (0,20±0,16 ng ml-1) dan kembali meningkat hingga mencapai konsentrasi tertinggi pada bulan Maret 2010. Tingginya konsentrasi estradiol-17p ini menunjukkan puncak vitelogenesis menuju maturasi. Hasil pengukuran biokimia plasma (total protein, kolesterol dan trigliserida, kecuali glukosa) yang rendah diperoleh pada bulan Juni 2009 (total protein 3,9±0,359 g dl-1; kolesterol 0,13±0,014 g dl-1; trigliserida 0,1±0,021 g dl-1) yang terjadi pada saat ukuran oosit mencapai maksimum. Konsentrasi glukosa terendah pada bulan September 2009 (0,04±0,019 g dl-1) saat ikan mengalami ovulasi, dan selanjutnya meningkat secara bertahap hingga mencapai optimal pada bulan Februari 2010 (0,12±0,003 g dl-1). Perkembangan kematangan gonad mencapai optimum pada bulan Juni dan September 2009.

Serotonin application in pregnant mare serum gonadotropin hormone and dopamin antagonist formulation to induce gonadal development of Indonesian tigerfish (Datnioides microlepis Bleeker, 1854) Bastiar Bastiar; Agus Oman Sudrajat; Melta Rini Fahmi
Jurnal Iktiologi Indonesia Vol 17 No 1 (2017): February 2017
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v17i1.302

The objective of study was to analyze the effect of serotonin (5-HT) in the formulation of hormones Pregnant Mare Serum Gonadotropin (PMSG) and dopamin antagonist (AD) hormones to gonad development of tigerfish. This study used completely randomized design (CRD) with five treatments of hormone namely: (P1) 1 ml of 0.9% NaCl (control); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG AD+10 mg+0.2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; and (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Each treatment was tested on five fish as individual replications. Hormone injected intramuscularly at the lower part of the dorsal fin of fish every 10 days. The fish were reared for 60 days. Fish that were used at this study were originate from natural catches as much as 25 fishes with 17.5-33.0 cm of total length and 118-926 g of body weight. During the study, fish fed using shrimp and small fish (live) twice daily at satiation. Measured parameters were gonadosomatic index (GSI), hepatosomatic index (HSI), 17P-estradiol (E2) plasma concentration and gonad maturity level based on morphology and histology examination. The results showed that the use of 2 mg of 5-HT are added to 20 IU PMSG and 10 mg AD (treatment P4) has stimulated the fish to had the highest GSI (2.38 ± 0.06%) and HSI (3,09±0,12%) which was significantly different to other treatment. The treatment (P4) could increase the E2 plasma concentration (37.14±2.99 pg.ml-1) two fold compared with the concentration before injection and stimulated the gonadal development to stage III. Abstrak Penelitian ini bertujuan untuk menganalisis pengaruh penggunaan serotonin (5- hydroxytryptamine atau 5-HT) dalam formulasi hormon Pregnant Mare Serum Gonadotropin (PMSG) dan antidopamin (AD) terhadap perkembangan gonad ikan ringau. Penelitian ini menggunakan rancangan acak lengkap dengan lima perlakuan (dosis.kg-1 bobot tubuh ikan): (P1) 1 ml NaCl 0,9% (kontrol); (P2) 20 IU PMSG+10 mg AD; (P3) 20 IU PMSG+10 mg AD+0,2 mg 5-HT; (P4) 20 IU PMSG+10 mg AD+2 mg 5-HT; dan (P5) 20 IU PMSG+10 mg AD+4 mg 5-HT. Setiap perlakuan diujikan pada lima ekor ikan sebagai ulangan individu. Penyuntikan hormon dilakukan setiap 10 hari dengan lama penelitian 60 hari. Hormon disuntikkan secara intramuskular pada bagian bawah sirip punggung ikan uji. Ikan yang digunakan merupakan hasil tangkapan alam dengan ukuran panjang total 17,5-33,0 cm dan bobot tubuh 118-926 g. Selama penelitian, ikan uji diberi pakan berupa udang dan ikan-ikan kecil (hidup) dua kali sehari secara satiasi. Parameter yang diamati adalah indeks kematangan gonad, indeks hepatosomatik, konsentrasi estradiol-17p plasma dan tingkat kematangan gonad berdasarkan morfologi dan histologi gonad. Hasil penelitian menunjukkan penggunaan 2 mg 5-HT dalam 20 IU PMSG dan 10 mg AD (perlakuan P4) menghasilkan perkembangan gonad yang lebih baik dibandingkan perlakuan lainnya. Nilai indeks kematangan gonad, indeks hepatosomatik, dan konsentrasi E2 plasma tertinggi diperoleh pada perlakuan P4 dengan nilai masing-masing sebesar 2,38±0,06%; 3,09±0,12% dan 37,14±2,99 pg.ml-1. Nilai tersebut berbeda nyata p<0.05) dengan perlakuan lainnya. Perlakuan P4 meningkatkan konsentrasi E2 plasma dua kali lebih besar dibanding-kan sebelum penyuntikan serta menghasilkan perkembangan gonad yang mencapai tingkat kematangan gonad tahap III.

Hormone profile of FSH, LH and estradiol with glucose blood level of Indonesian short-finned eel (Anguilla bicolor bicolor Mc Clelland, 1844) stimulated by HCG, MT, E2 and dopamine inhibitory Abdul Zahri; Agus Oman Sudrajat; Muhammad Zairin Junior
Jurnal Iktiologi Indonesia Vol 18 No 1 (2018): February 2018
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v18i1.374

This study aims to analyze the hormone profile of FSH, LH and E2 to the eel after exogenous hormone stimulation and blood glucose levels. Six formulated treatment applied with combination of dopamine antagonize 10 mg.mL–1 (A), estradiol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hCG 2 mg.mL–1 + EA (hEA) and hCG 2 mg.mL–1 + MTA (hMTA), with (F) physiologis 0.9% NaCl to control. Six group eel (200±15g) reared in a concrete tank with a capacity of 3,400 liters and filled with sea water of 35 mg L–1 as much as 2000 liters. Eels injected 1 mL.kg–1 hormone by intramusculary, were feed to apparent satiation daily for 10 weeks. The study used Completely Randomized Design with one treatment factor, namely hormonal factor and its combination. Fish blood that ware directly concentration to FSH, LH and E2, the enhanced significantly high in the blood plasma on treatment hMTA and hEA P<0.05. Glucose concentration in the blood palsma is high enough in a row on a formula hMTA 67.33 mg.dL–1 and significantly different to P<0.05. The result indicates that induction of exogenous hormone (hMTA) improve FSH, LH and E2. FSH and LH profiles show permutation patterns during the development of eel gonad, beginning with the increase of FSH in the early phases of gonadal development. LH profile moves in line with the increase in E2 during gonadal maturation process eels (A. bicolor bicolor), with blood glucose levels in the normal range. Abstrak Penelitian ini bertujuan menganalisis profil hormon FSH, LH dan E2 ikan sidat yang dirangsang dengan hormon ekso-genous serta kadar glukosa darah. Enam perlakuan diterapkan dengan kombinasi anti dopamin 10 mg.mL–1 (A), estra-diol (E2) 3 mg.mL–1 + A (EA), metyltestosteron (MT) 3 mg.mL–1 + A (MTA), hormon hCG 2 mg.mL–1 + EA (hEA) dan hCG 2 mg.mL–1 + MTA (hMTA), dengan kontrol (F) fisiologis 0,9% NaCl. Enam kelompok sidat uji (200±15 g) dipelihara pada bak beton berkapasitas 3.400 liter dan di isi dengan air laut bersalinitas 35 mg L–1 sebanyak 2000 liter. Sidat diinjeksi dengan dosis hormon 1 mL.kg–1 secara intramuskular, hewan uji diberi pakan secara at satiation sekali sehari selama 10 minggu. Penelitian menggunakan Rancangan Acak Lengkap dengan satu faktor perlakuan, yaitu faktor hormon dan kombinasinya. Darah sidat terbukti mengandung FSH, LH, E2, peningkatannya terlihat nyata terjadi mela-lui plasma darah pada perlakuan hMTA dan hEA pada tingkat P<0,05. Konsentrasi glukosa tertinggi pada formula hMTA 67,33 mg.dL–1 dan berbeda nyata pada taraf P<0,05. Hasil ini menunjukkan bahwa penambahan hormon ekso-genous perlakuan hMTA meningkatkan konsentrasi FSH, LH, dan E2. Profil FSH dan LH menunjukkan pola permutasi selama proses perkembangan gonad sidat, di mulai dengan meningkatnya FSH di fase awal perkembangan gonad. Profil LH bergerak seiring dengan peningkatan E2 selama proses pematangan gonad sidat (A. bicolor bicolor), dengan kadar glukosa darah pada kisaran normal.

Performance of Pregnant Mare Serum Gonadotropin and Vitamin Mix in Inducing Striped Catfish Pangasianodon hypophthalmus Rematuration Syifania Hanifah Samara; Citra Fibriana; Uthami Nagin Lestari; Agus Oman Sudrajat
AQUASAINS Vol 7, No 2 (2019)
Publisher : Jurusan Perikanan dan Kelautan

The research aimed to assess the performance of Pregnant Mare Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (HCG) hormone injection combined with vitamin mix to induce the rematuration of Striped catfish Pangasianodon hypophthalmus broodstock. Three PMSG and HCG treatments of 5 and 2,5 IU; 10 and 5 IU;also 20 and 10 IU/kg of broodstock body weight dosage combined with vitamin mix addition to fish feed and one control was used with four replications. The broodstock was reared for four weeks and weekly sampling for GR and SGR as well as hormone injection were performed. For the following two weeks, the broodstock was observed for maturation. Artificial spawning was conducted for broodstock reaching gonadal maturity. Statistic analysis of observed variables showed insignificant differences among the three treatments in rematuration parameters. The rematuration rate resulted in a 100% pregnancy. Broodstock treated with 10 IU PMSG and 5 IU HCG/kg of spawner body weight was able to reach maturity and spawned with the larval survival rate on the fourth day at 84,81%, thus it can be concluded that 10 IU PMSG and 5 IU HCG/kg of body weight as the best dosage.

OPTIMAL ELECTROPORATION CONDITION FOR SPERM MEDIATED GENE TRANSFER IN STRIPPED CATFISH (Pangasionodon hypophthalmus) Raden Roro Sri Pudji Sinarni Dewi; Alimuddin Alimuddin; Agus Oman Sudrajat; Komar Sumantadinata; Sularto Sularto
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

The success of transgenic fish production has been achieved through eggs fertilization using electroporated sperms carrying exogenous DNA. This study was conducted in order to obtain the optimal electroporation condition for stripped catfish sperm. A plasmid containing green fluorescent protein (GFP) gene driven by carp β-actin promoter was transferred into sperm using electrophoresis method towards transgenic stripped catfish (Pangasionodon hypophthalmus) production. Electroporation was carried out using square wave shock with pulse length of 30 ms and pulse interval of 0.1 sec. Treatments are combination between voltage (50 V, 75 V, and 100 V) and pulse number (1 and 3). Exogenous DNA concentration used was 10 μg/mL of Tris-EDTA. Results showed that increasing the voltage from 50 to 100 decreased sperm motility, while pulse number did not affect sperm motility. Voltage of 50 gave the best motility of sperm, although sperm viability relatively similar between treatments and control except at 100 V with 3 pulses number. Further, electroporation-treated sperms were able to fertilize eggs. Higher hatching rate of eggs was obtained in electroporation treatment at 50 V with pulse number of 1 and 3. The persistence of transferred GFP was detected in electroporated and incubated sperms (control). However, GFP was only detected in larvae from eggs that were fertilized by electroporated sperm. Thus, electroporation could be applied to produce transgenic stripped catfish.

Co-Authors , Alimuddin . Suriansyah Ade Yulita Hesti Lukas Afif Abdurrahman Agoes Mardiono Jacoeb Akbar, Muhamad Saepul Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimudin Alimudin, Alimudin Andin Dwi Fitri Rahayu Andri Yanto Andriyanto Andriyanto Andriyanto Andriyanto Antharest Sugati, Antharest Aprelia Martina Tomasoa, Aprelia Martina Apriana Vinasyiam Arief Boediono Arlita, Kriswidya Armen Nainggolan Asda Laining Awaludin Bambang Gunadi Bambang Gunadi Bambang Gunadi Bastiar Bastiar Benny Heltonika Citra Fibriana Cut Dara Dewi Damiana Rita Ekastuti Daniel Djokosetiyanto Deni Radona, Deni Deny Sapto Chondro Utomo Didik Ariyanto Didik Ariyanto Dinamella Wahjuningrum Dinar Tri Soelistyowati Dony Prasetyo Dwi Mulyasih Dythia Asma Nadia Edison, Thomas Eka Kusuma Eko Harianto, Eko Eko Rini farastuti Enang Harris Eni Kusrini, Eni Eny Heriyati Enzeline, Valensia Epro Barades Erma Primanita Hayuningtyas Fahmi Akbar, Fahmi Fauzan Fajari Gustiano, Rudhy Hadra Fi Ahlina Hafidah, Riva Hafif Syahputra Halawa, Nenima Hanggara, Yudha Harton Arfah Haryanti Haryanti Haya Audina Azizia Helena Sahusilawane Herawati Rasid Hesti Wahyuningsih Hirmawan Tirta Yudha Iis Diatin Imron Indra Lesmana Irvan Faizal Irvan Faizal Irzal Effendi Jonathan Felix Raynar Joni Haryadi, Joni Jr, Muhammad Zairin Jufri, Fatahillah Maulana K. Sumawidjaja Kapelle, Imanuel B. D. Klea Chandra Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Ligaya I. T. A. Tumbelaka Luki Abdullah M. Zairin Junior Mahdaliana, Mahdaliana Mala Nurilmala Maulana, Fajar Megawati Wijaya Meillisa Carlen Mainassy Melta Rini Fahmi Mia Setiawati Mochamad Syaifudin, Mochamad Mohammad Mukhlis Kamal MUHAMMAD AGUS SUPRAYUDI Muhammad Cito Khairel Fauzan Muhammad Junior Zairin Muhammad Muttaqin Muhammad Zairin Jr Muhammad Zairin Jr Muhammad Zairin Jr. Muhmmad Agus Suprayudi Mulyani, Lina Muslim Muslim N. Suhenda Nadia Mega Aryani, Nadia Mega Nazar, Danella Austraningsih Puspa Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Odang Carman Olivia Karo Permana, Andre Puji Hastuti, Yuni Pustika Ratnawati Putri Utami, Putri Raden Roro Sri Pudji Sinarni Dewi Raden Roro Sri Pudji Sinarni Dewi Raden Roro Sri Pudji Sinarni Dewi Rahmi, Kurnia Anggraini Ratu Siti Aliah Rezki, Dinda Wahyu RIDWAN AFFANDI Rizsa Mustika Pertiwi Ronny I. Wahju S. Sarah Samara, Syifania Hanifah Sandra, Aan Aryanti Sholihin, Hidayatush Sinansari, Shofihar Siti Subaidah Sri Nuryati Sri Nuryati Sufal Diansyah Sularto Sularto Suriansyah Suriansyah Suriansyah, Suriansyah T. Budiardi T. Nursyams T. Prasetya Tatag Budiardi Tuti Puji Lestari Upmal Deswira Uthami Nagin Lestari UTUT WIDYASTUTI Wahyu Purbiantoro Wasmen Manalu Wibowo, Kesit Tisna WIDANARNI WIDANARNI Wildan, Dudi Muhammad Wiwin Kusuma Atmaja Putra Y. Hadiroseyani Yanti Sinaga Yonvitner - Zahri, Abdul

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