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Activity Test of Extracts of Phyllantus Herbs (Phyllantus niruri L.) and Black Seeds (Nigella sativa L.) to The Active Cutaneous Anaphylactic Reaction in Male Wistar Rats in The Induction of Ovalbumin Arisma Nuri Pebryana; Gunawan Pamudji Widodo; Yul Mariyah
Jurnal Farmasi Indonesia Vol 11 No 2 (2014): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1296.743 KB) | DOI: 10.31001/jfi.v11i2.70

Abstract

Anaphylactic reactions is caused by repeated exposure to the same allergen mediated by specific IgE attached to mast cell and basophils wall. The administration of Phyllantus niruri L. and Nigella sativa L. have function as antiinflamation and immunomodulator. The study was purposed to prove the effect of ethanol extract of Phyllantus herbs and black seeds as inhibitors of active cutaneous anaphylactic. This study used 25 rats. Allergic rats was induced by ovalbumin (OVA) 0,1% and 0,25% in Al(OH)31% subcutaneously. Rats were divided into 5 groups : CMCNa 0,5% (negative control), sodium cromolyn 2 mg/kg BB (positive control), a single dose of Phyllantus herbs 81 mg/kg BB, a single dose of black seeds 30 mg/kg BB, the combination dose of Phyllantus herbs and black seeds (40,5:15 mg/kg BB). On the days 21th, backs rats were shaved 2,5 x 4 cm, and it were injected by evans blue 1,5%. The analyzed data was the blue pigmented areas. The result showed that treatment groups was differed significantly the negative and positive control group (p<0,05). The combination of ethanol extract phyllantus herbs and black seeds provide the most effective barrier anaphylaxis because it’s ability to inhibit the activation of mast cell adequately.
Uji Aktivitas Ekstrak Etanol Herba Sambiloto (Andrographis paniculata Nees) dan Biji Jinten Hitam (Nigella sativa L.) terhadap Reaksi Anafilaksis Kutan Aktif pada Tikus Putih Wistar Jantan yang Diinduksi Ovalbumin Yeli Trimayanti; Gunawan Pamudji Widodo; Yul Mariyah
Jurnal Farmasi Indonesia Vol 12 No 1 (2015): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (611.689 KB) | DOI: 10.31001/jfi.v12i1.80

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Development of medical plants for the allergic reaction is continuing with the extrac of the bitter (Andrographis paniculata Nees) and black seed (Nigella sativa L.) as an example. This reseach’s purpose is studying inhibition of anaphylaxis reactions effect from ethanol extracts of the bitter herbs and black seed for active anaphylaxis reaction inducted by ovalbumin in male Wistar rats. Research used active anaphylaxis reaction method with 5 test groups, i.e. negative control group, positive control group and 3 tread group. The rats were subcutaneously sensitized by ovalbumin two act time with an interval of seven days. Back rats shavered and intravenaosly injected with evans blue in rats tail. Diameter area of pigmentation measured every hour, up to 8 hours. Obtained data then tested statistically using ANOVA. Results showed that the extract of bitter herbs (120 mg/kg BB), black seed (30 mg/kg BB) and combination of both (60 mg/kg BB : 15 mg/kg BB) could inhibit pigmentation in the back rats skin area. Percent inhibition respectively is 12.39 %; 14.51 %; 22.36 %. Nevertheless, the inhibitory effect was still lower compared with cromolyn sodium as an mast cell stabilizer drug. It can be concluded that the combination of bitter herbs ethanol extracts with black seed ethanol extracts more effectively inhibit active cutaneous anaphylaxis reaction of ovalbumin induced in male Wistar rats.
AKTIVITAS ANTIJAMUR KRIM EKSTRAK ETANOL DAUN SIRIH MERAH (Piper betle L. var Rubrum) PADA KELINCI BETINA YANG DIINFEKSI Candida albicansATCC® 10231 Dwi Ningsih; Aneng Ardiya Sukmawanti; Gunawan Pamudji Widodo
Jurnal Farmasi Indonesia Vol 12 No 2 (2015): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.763 KB) | DOI: 10.31001/jfi.v12i2.87

Abstract

Piper betle L. var Rubrumbe one of herbal that have historically been used as an antifungal. Flavonoid, alkaloid, saponin, tannin, and essential oils that consisting in it was expectedhave antifungal activity. Thestudy aimto determine theantifungal activity ofPiper betle l. var Rubrumleaves ethanol extract creamon female rabbit infectedCandida albicans ATCC® 10231. The antifungal experiment of Piper betle L. var Rubrumdone by infecting vagina New Zealand White rabbits with Candida albicans ATCC® 10231. Forty-eight hours after infection, vaginal smeared with 96% ethanol extract of Piper betel leaf concentration of 2.5%, 5%, 10%, and ketoconazole 2% as a positive control. Clinical symptoms that arise in the rabbit vagina during healing were observed and the number of fungal colonies of rabbit vagina calculated. The data obtained were analyzed with Anova methods with significant level of 95%, followed by SNK test. The results showed that 96% ethanol extract cream Piper betel leaf concentration of 2.5%, 5%, and 10% have antifungal effect. Cream with a 10% concentration was the most effective as an antifungal.
EVALUASI PERENCANAAN KEBUTUHAN OBAT PUBLIK SERTA KETERSEDIAAN OBAT DI TINGKAT PUSKESMAS SE-WILAYAH KERJADINKES KOTA SURAKARTATAHUN 2015 Eko Yudha Prasetyo; Satibi .; Gunawan Pamudji Widodo
Jurnal Farmasi Indonesia Vol 13 No 2 (2016): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (658.166 KB) | DOI: 10.31001/jfi.v13i2.289

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Drug planning is an important aspect of drug management. Planning is aimed at determining the type and amount of drug in accordance with the needs of community helth center. Although the regulatory guideline of public drug management have been made, but still found the incidence of overstock and stockout. With these considerations it’s necessary to evaluate related planning processes and drug availability. The purpose of this study to know the planning process, the problems encountered and the level of drug availability in community health center throughout the whole working area of Surakarta Department of Health. This research is observational and evaluative study with qualitative approach, supported by quantitative data. Secondary data were obtained from the search document of drug plan for 2015, the usage and demand report of drug. The secondary data is processed to determine the accuracy of planning, the availability and suitability of drug demand. To strengthen information were conducted triangulation and interviews with drug manager related. The results showing the drug plan has not been efficient seen from an average accuracy of drug planning in treatment primary health centers is 440.5% and 389.98% at non-treatment primary health centers. The problems in planning isn’t optimal use of information management system based on electronically in providing data for drug planning, the procurement with e-purchasing requires time and administration are too long, the plan of medicine isn’t fully realized, the community health center received drug beyond the plan so that the use of drugs less effective, The average availability of drugs in the category of excess which amounted to 31.13 months in treatment health centers and 33.95 months in non-treatment.
EFFECT OF KNOWLEDGE LEVEL TO QUALITY OF LIFE WITH DRUG USE COMPLIANCE AS INTERMEDIATE VARIABLE AT RHEUMATOID ARTHRITIS PATIENTS IN OUTPATIENT PHARMACY DEPO Dr. MOEWARDI HOSPITAL SURAKARTA Rahmadani .; Chairun Wiedyaningsih; Gunawan Pamudji Widodo
Jurnal Farmasi Indonesia Vol 13 No 2 (2016): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.237 KB) | DOI: 10.31001/jfi.v13i2.290

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Prevalensi rheumatoid arthritis di berbagai negara termasuk Indonesia meningkat sejalan dengan perubahan gaya hidup seperti asupan makanan, obesitas, kurangnya aktivitas fisik, dan obat-obatan. Peningkatan pengetahuan dan kepatuhan penggunaan obat diharapkan dapat mempengaruhi peningkatan kualitas hidup pasien rheumatoid arthritis. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh tingkat pengetahuan terhadap kualitas hidup dengan kepatuhan penggunaan obat sebagai variabel antara. Penelitian menggunakan metode cross sectional, dengan survey untuk melihat pengaruh tingkat pengetahuan terhadap kualitas hidup dan kepatuhan penggunaan obat sebagai variabel antara. Penilaian pengetahuan menggunakan ACREU RAKQ, penilaian kepatuhan menggunakan MMAS-8 dan penilaian kualitas hidup menggunakan The AIMS2-SF. Data yang diambil adalah data tingkat pengetahuan, kepatuhan penggunaan obat dan kualitas hidup dengan menggunakan kuesioner dan data dianalisis menggunakan path analysis. Subyek yang diperoleh dalam penelitian sejumlah 30 pasien rheumatoid arthritis. Hasil kuesioner tingkat pengetahuan yaitu didapatkan 13 responden (43,33%) yang memiliki pengetahuan tinggi. Hasil kuesioner kepatuhan penggunaan obat yaitu didapatkan 14 responden (46,67%) yang memiliki kepatuhan sedang. Hasil kuesioner kualitas hidup yaitu didapatkan 16 responden (53,33%) yang memiliki kualitas hidup sedang. Pengaruh signifikan antara tingkat pengetahuan terhadap kualitas hidup dengan kepatuhan penggunaan obat sebagai variabel antara dengan nilai estimasi atau besaran persentase pengaruh 79%, artinya kepatuhan penggunaan obat berpengaruh signifikan.
Aktivitas Antitusif Dan Ekspektoran Ekstrak Etanol, Fraksi Polar-Semi Polar Herba Meniran (Phyllanthus niruri L.) Pada Marmut (Cavia porcellus) Fakhruddin Fakhruddin; Arief Nurrochmad; Gunawan Pamudji Widodo
Jurnal Farmasi Indonesia Vol 14 No 2 (2017): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.559 KB) | DOI: 10.31001/jfi.v14i2.296

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Phyllantus niruri L., is wild plants, has wide applications in traditional medicine. The purpose of this study is to evaluated antitussive, expectorant activity, and description antitussive mechanism of phyllantus herbs. Test animal male guniea pigs as many as 36 divided into 9 groups each consisting of 4 guinea pigs. The antitussive activity of ethanol extract and polar-semi-polar fraction be observed on percentage cough suppression by giving a citric acid 7.5% for 5 days. The Expectorant activity was evaluated with phenol red concentration in guinea trachea on 7 days. The mechanism description is evaluated by looking at the comparison of phyllantus herbs and combination ethanol extract phyllantus herbs and codeine modulated by the opioid receptor and glibenclamide modulated by the ATP sensitiv.e K+ channel. The research showed ethanol extract and polar-semi-polar fraction increased the suppression of citric acid induced by citric acid 7.5% and decreased the a supprssant of mucus secretion on evaluation of expectorant. Polar-semi-polar fraction 160 mg/kgBB showed the effective activity antitussive 63,57%. Polar-semi-polar fraction 80 mg/kgBB showed decreased phenol red secretion by 56,91%. This study provides evidence that phyllantus herbs can be used as an antitussive and a suppressant of mucus secretion. Phyllantus herbs not description on mechanism antitussive modulated by the opioid receptor and ATP sensitiv.e K+ channel. The antitussive mechanism may be modulated by other systemic nerves and peripheral nerves
Uji Aktivitas Analgetika Fraksi-Fraksi Ekstrak Etanol Daun Gandarusa (Justicia gendarussaBurm. f) dengan Metode Tail Flick Rosa Juwita Hesturini; Rina Herowati; Gunawan Pamudji Widodo
Jurnal Farmasi Indonesia Vol 15 No 1 (2018): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (524.439 KB) | DOI: 10.31001/jfi.v15i1.346

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ABSTRACTGandarusa (Justicia gendarussa Burm. f) is a potential plant as analgetic activities.The aim of the present research was to investigate the analgetic activity with tail flick method onrat models of ethanol extract and the fractions of gandarusa leaf.Partitioned fractinations isolated from ethanolic extract of gandarusaleaves with nheksana,etil asetat and water. Dosage extracts and fractions gandarusa leaf, respectively, are250 mg/kg bw, 500 mg/kg bw, n-hexane fraction of 314 mg/kg bw, the fraction of 41,05 mg/kgbw and water fractions 144,8 mg/kg bw, the negative control group CMC and aspirin 360 mg/kgbw. The results showed the n-heksana fraction has optimal analgesic activity withantinociceptive percentage 94,58% comparable to aspirin.The preliminary phytochemical analisys showed the presence of active phytochemicalsuch as flavonoids, alkaloids, saponins, steroids dan tannins and these compound might beresponsible for analgetic mechanism. The possibility analgetic mechanism of the gandarussa itsstimulate lipomodulin protein biosynthesis which inhibit the action of phospolipase enzimaticthat affecting the enzyme activity arachidonic acid but there is also has gastroprotective activitywith increased mucus production and prevents the release of histamine.
Analisis Biaya Terapi pada Pasien Infeksi Saluran Kemih Rawat Inap di RSUD Dr. Moewardi Surakarta pada Tahun 2016 Windi Astuti; Gunawan Pamudji Widodo; Rina Herowati
Jurnal Farmasi Indonesia Vol 15 No 1 (2018): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (513.364 KB) | DOI: 10.31001/jfi.v15i1.353

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Urinary tract infection is one of the infectious diseases that occur due to the presenceof microorganisms in the urine. Urinary tract infection financing was regulated in the INA-CBG’srates. The study purpose was determined the conformity of real cost to the INA-CBG’s rates inhospitalized of national health insurance patients with urinary tract infection disease in Dr.Moewardi hospital Surakarta in 2016.This study was an observational study using a cross sectional design. Retrospectivedata collection method. Data were analyzed to see the treatment pattern of patients duringhospitalization, to find the difference in real costs with INA-CBG's rates using the one sample ttest,and to see factors related to real costs using the bivariate correlation test.The result showed that the treatment pattern for hospitalized patients with urinary tractinfection are using Ceftriaxone (40,8%), Cefixime (7,1%), Ceftazidime (10,2%), Ciprofloxacin(26,5%), Levofloxacin (9,2%), Amoxicillin (3,1%), Ampicillin (1,0%), and Gentamicin (2,0%). Theanalysis of real cost to the INA-CBG’s rates showed the differences between real cost and INACBG’srate of national health insurance patients with urinary tract infection disease.This difference shows a positive condition, where the total real costs are lower than the INACBG'srates. The factor related to the real cost of urinary tract infection treatment including ofLOS, secondary diagnoses, and treatment classes.
Efek Ekstrak Etanol Daun Kelor (Moringa oleifera, Lamk.) Pada Mencit Model Demensia: Kajian Memori Spasial, Kadar Malondialdehid Dan Jumlah Sel Piramidal Hipokampus Area CA1 Dan CA2-CA3 arikha ayu susilowati; Gunawan Pamudji Widodo; Jason Merari
Jurnal Farmasi Indonesia Vol 16 No 2 (2019): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (868.86 KB) | DOI: 10.31001/jfi.v16i2.612

Abstract

ABSTRACT Dementia is a disease of nerve cells in the brain, due to the hippocampus damaged by free radicals. EEDK (Ethanol extract of kelor leaves)(Moringa oleifera, Lamk.) contains phytochemicals, carotene, vitamins, minerals, amino acids, flavonoids and phenolics, proven to reduce oxidative damage and memory deficits. This study aims to determine the effect of EEDK on spatial memory, MDA levels, the number of hippocampal pyramidal cells damaged in the CA1, CA2-CA3 area and histopathological features of mice. The design of this study was pre-test and post-test control group design for spatial memory test and post test control group design for MDA levels, hippocampal pyramidal cell counts and histopathological features. This Reseach using 30 mice divided into of 6 groups, each group consist of 5 mice, namely the normal control group, positive control, negative control, EEDK group 100, 200, and 400 mg/kg body weight, all groups induced by Pb acetate 50 mg / KgBB except the normal group. Positive control was treated with quercetin 50 mg/kgBB, while negative controls were not treated. Memory test data obtained from the Morris water maze pre and post-test, antioxidant status data obtained from MDA levels and histopathological data obtained from the calculation of pyramidal cell counts. Data analysis used repeated measures ANOVA and ANOVA with Post Hoc LSD. The results showed that latency time, MDA level test and brain histopathology examination had significant differences between the negative control group and the EEDK group. EEDK 400 mg/kgBB was most effective in improving spatial memory, decreasing MDA levels, and preventing damage to the hippocampal pyramidal cell CA1, CA2-CA3 area.
Uji Aktivitas Diuretik Ekstrak n-Heksana Daun Ceplukan (Physalis angulata L.) pada Tikus (Rattus norvegicus) Jantan Wistar INDRAWATI FITRIANA; AGUNG ENDRO NUGROHO; GUNAWAN PAMUDJI WIDODO
Jurnal Farmasi Indonesia Vol 9 No 1 (2012): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1009.524 KB) | DOI: 10.31001/jfi.v9i1.714

Abstract

Ceplukan (Physalis angulata L.) empirically have diuretic effect. This study aimed to determined the diuretic effect of n-hexane extract of ceplukan leaves on Wistar male rats. Ceplukan leaves powder were extracted by maceration with n-hexane solvent. Test animal was used 25 Wistar male rats were divided into 5 groups: group I were given CMCNa 0.5% (negative control), group II (positive control) were given furosemide dose 4.32 mg/200 g BW, group III, IV, V were given n-hexane extract suspension dose of 375, 187.5, 93.75 mg/200 g BW respectivelly. The urine volume were measured in hours-6, 12, 18 and 24. Diuretic effect is obtained by calculating the cumulative urine volume and AUC (Area Under the Curve). The data obtained were analyzed by one way ANOVA with a level of 95% significant, then test by LSD (Least Significant Difference).The results showed that n-hexane extract of ceplukan leaves had diuretic effect. The effective dose of n-hexane extract of ceplukan leaves for diuretic effect was dose 375 mg / 200 g BW.
Co-Authors Abdul Mahmud Yumassik abdul rofiq Achmad Fudholi Achmad Fudholi Ade Sukma Hamdani Adrianto, Madyo Agnes Christina Budiwati Agung Endro Nugroho Agung Endro Nugroho Agung Endro Nugroho Andayani, Tri Murti Aneng Ardiya Sukmawanti Anggun Setya Wibawa, Anggun Setya Arief Nurrochmad arikha ayu susilowati Arisma Nuri Pebryana Aslam, Astri Asri Laksmi Riani Asri Laksmi Riani Astri Aslam Aswandi Aswandi Ayu Wulandari Ayudia Cipta Khairani BETA K. PRAMUDYAWARDHANI Chairun Wiedyaningsih Chandra Arifin Clara Ritawany Sinaga Dan Mona Aprilia Daniel Maharai Daniel Maharai Dedent Eka Bimmaharyanto.s Dewi, Lucia VIta Inandha Dian Marlina Dini Okyaviani Dwi Ningsih Eko Yudha Prasetyo Elin Yulinah Sukandar Elina Endang Sulistyowati Endang Diyah Endang Diyah Endang Dyah Ikasari endey, joverly Ercis Ercis Ercis, Ercis Fauziyyah Al Hasanah Fendy Prasetyawan FEOLISTIN MIENCE POBAS Ferdinan Jalung Ferdinan Jalung Fitrianingsih, Sri Fridly Manawan Gunawan Firmansyah Gusti Ayu Rai Saputri Handayani, Sri Rejeki Handi Mukti Fahrizal Handi Mukti Fahrizal Haris Maspekeh Hartih, Nur Aeni Hartono Hartono Hasbrianti, Armayanti Rindiarko Hastuti, Nita Hasty Martha Wijaya Hierowat, Rina Humaidah, Daffa I Ketut Adnyana Ika Purwidyaningrum INDRAWATI FITRIANA Irmatika Hendriyani Islamiati , Utami Ismi Rahmawati Isna Nur Khasanah Iswandi Iswandi Iswandi Iswandi Ittiqo, Dzun Haryadi Iwan Yuwindry Jason Merari Peranginangin Junagsti Bermalam Kartini Nur Wulandari Latief, Aprilianti A. Lestari, Rizkia Putri Madyo Adrianto Madyo Adrianto Mamik Ponco Rahayu MARIA E.D.O. HUREK MAKING MARIA YANGSYE LENGGU Marlina, Dian Milda Rianty Lakoan Milda Rianty Lakoan Muhamad Aulia Putra Tawakal Muhammad Syukri Mukti, Yusuf Anggoro Naha Ndjurumbaha, Echa Malinda Noval Noval Noval Noval Nugroho , Agung Endro Nugroho Wisnu Putro Nur Aeni Hartih NURAINI HARMASTUTI Nuraini Harmastuti Nuraini Harmastuti Oetari Oetari Oetari Oetari Pare, Nurdiana Tandi Poppy Dwi Citra Jaluri Purwidyaningrum, Ika Ika Puspitasari, Ismi R, Herowati R., Wirdah Wati R.A. Oetari R.A. Oetari R.A. Oetari, R.A. Rahmadani . Rahmawati, Ismi Ranowijaya Ranowijaya Recky Patala Rika Arfiana Safitri Rosa Juwita Hesturini Ruly Anggrayni Safwan Safwan, Safwan Santi Dwi Astuti Satibi Satibi . Satibi Satibi Satibi Satibi Satibi Satibi Siswanto, Didik Sri Fitrianingsih Srini, Dwi Sukoharjanti, Bintari Tri Sukrasno Sukrasno Suryono Suryono Teodhora, Teodhora Teuku Nanda Saifullah Sulaiman Theedens, Maria Theodora Toding, Ficanata Adhiguna Tri Murti Andayani Tri Murti Andayani Tri Wijayanti Tri Wijayanti Valiandri Puspadina Wahdah, Rismaul Wahid, Abdul Rahman Widayatul Khairi Widia Wati Widyasti, Jena Hayu Windi Astuti Wirdah Wati R. Yeli Trimayanti Yul Mariyah Yuwindry, Iwan Zamzani, Irfan