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Journal : Medical Journal of Indonesia

 1 
Touch DNA viability on various substrates from different shedder levels Saamia, Vira; Yudianto, Ahmad; Nurjayadi, Muktiningsih; Novitasari
Medical Journal of Indonesia Vol. 33 No. 3 (2024): September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247398

Abstract

BACKGROUND Touch DNA samples are frequently discovered at crime scenes, including those found at the scene, on the victim, with the suspect, or on objects related to the incident. This study aimed to investigate 3 key factors affecting touch DNA samples: the characteristics person that shed the DNA, surfaces variants where the DNA was deposited, and different sampling methods effectiveness that influence DNA quantity, quality, and detection. METHODS 9 participants grouped into high, intermediate, and low shedder levels simultaneously tied 2 types of ropes, non-porous and porous. The first person will hold a rope for 5 min then pass it to the second person to hold on the same spot for another 5 min. DNA was collected from each rope using the double swab and tape-lift method, extracted, and quantified using real-time polymerase chain reaction (PCR). Touch DNA profile at 20 short tandem repeat loci was amplified in PCR system and detected on capillary electrophoresis. RESULTS Type of substrate (p = 0.97) or sampling method (p = 0.053) used for touch DNA collection did not significantly impact the DNA yield or profiling outcomes. A notable difference (p<0.001) was found in DNA quantity between high, intermediate, and low shedders, regardless of the substrate or method used. CONCLUSIONS Individual shedder level has a greater influence on the results of touch DNA analysis regarding the DNA quantity and profiling quality than substrate type and sample procedure.
Co-Authors Abomoelak, Bassam Adel Paila Adiprabowo, Vani Dias Ahmad Nur Kholis Ahmad Taufik Ahmad Yudianto Alan Su'ud Ma'adi Ali Rokhman Andi Aulia Raodatul Husna Andi Bau Mallarangeng Andre Pratama Sitepu Anwar, Agus Saeful Arief, Muhamad Asmala Sabrina Ayu Dwi Putri Rusman Baso Anugrah Hasanuddin Cahyani, Hilda Charenina Tsabitha Amanda Chintya Dewi Chonitatun Nisak Darlan Sidik Delfitriani Dhea Amanda Rowandita Dini Ayuning Ratri Sukimin Edy Herianto Ekowati, Lia Elisa Mayang Sari Etty Zuliawati Zed Faizati umu Aimanah Fitriana Amelia Fitriyanti, Anisa Hamdan Hidayat Hanur, Binti Suaidah Hetty Rohayani Holdani Kurdi I Made Budiarsa I Nengah Kundera Ihsan Syah Putra Ikhsan Maulana Indah Riezky Pratiwi Irayani, Surya Irianto Isnaini Nur Safitri Iwan Satriyo Nugroho Jayanti, Zulaikhah Dwi Jefferson Lynford Declan Juninta, Tania KHOIRUNNISA Khumaidi Kurniawansyah, Edy Kurniawansyah, Kevin Lesmana, Hans Listiawati, Rodiana Luhjingga Panasari Urbaningrum Lulu Hafizah M. Ismail Manap Trianto Marbun, Jhony Marthiawati, Noneng Mas'ud, M. Imron Mia Rasmiaty Monik, Della Tiara Muhamad Arief Muhammad Irfan Hakim Muhammad Maulana Muhammad Sofwan Muktiningsih Nurjayadi Mulyono Nailia, Nur Nawang Anggi Nefianto, Tirton Neneng Gantini Nur Azlina Nurfansyah Nursyamsi Hamza Prahasti Suyaman Puteri, Made Widya Wirayanti Putri, Gladys Indira Ramadhani, Arie Ramadhani, Muhammad Aulia Ria Arafiah Riana T. Mangesa Riki Ruswandi Rodiana Listiawati, Rodiana Rohil Firdaus Roudhotul Jannah Rubianto, Aisyah Vanadia Ruswandi, Riki Saadah Yuliana Saamia, Vira Sabrina Fania Saefuloh Salsabila Al Muniroh Sampir Andrean Sukoco Samsurizal, M. Sulaeman Sanita Dhakirah Septa, Badai Sholihah, Izzatus Sintia Hepi Andini Siregar, Risdalina Sitanaya, Rini Siti Kusnul Khatimah Sri Harmuli Suhel Sulaeman Sunaka, I Wayan Sutandar, Vivi Hendra Tata Wijayanta Ulfa Ramadhani Usman Usman Vidyasari, Rahmanita Zuhal Azzamul A'la