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All Journal Majalah Kedokteran Bandung Media Kedokteran Hewan Majalah Obstetri dan Ginekologi Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Veterinary Biomedical and Clinical Journal Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences.
Boedi Setiawan
Departemen Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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GAMBARAN PATOLOGI HEPAR IKAN LELE DUMBO (Clarias gariepinus) YANG DIPAPAR LOGAM TIMBAL NITRAT Pb(NO3)2 Dellavia, Yolanda; Widiyatno, Thomas Valentinus; Setiawan, Boedi; Arimbi, Arimbi; Poernomo, Bambang; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21092

Abstract

This research aimed to know the changed damage of liver African catfish (Clarias gariepinus) were exposed by lead nitrat. This study used twenty four of African catfish (Clarias gariepinus) with average weight of 20-25 grams, size 10-12 cm, age ± two months. This research was designed by a completely randomized design (CRD). All member of population of the African catfish were divided into six groups, consist of six repetitions each, namely P1, P2, P3, and P0 as a negative control respectively.  P1 was given with dosage of lead nitrat 7,26 mg/liter, P2 was given with dosage of lead nitrat 14,53 mg/liter, and P3 was given with dosage of lead nitrat 29,06 mg/liter. According to the macroscopic observed, show that liver became swollen and pale.  The histopathological features of hepar were examined under light microscope in 400 times magnification. Scoring method were using Bernet Scoring Method to examined the presence of degeneretion, congesti, necrotic, and infiltration of leukocyte. Then, Kruskal-Wallis test through with Mann-Whitney test of statistical analysis. The statistical analysis showed the median number of P0 (0,05), P1 (0,45), P2 (1,10), and P3 (1,40) respectively. From the result can be concluded lead exposure with dose 29,06 mg/liter severe which was heavily histopatological in hepatocytes cell of african catfish (Clarias gariepinus) liver.
Acute Toxicity Test Of The Green Viper Snake (Trimeresurus albolabris), Macroscopic Description Of The Kidney And Liver Of Mice (Mus musculus) Darmawan, Moch. Rizky; Rahardjo, Dadik; Tyasningsih, Wiwiek; Kurnijasanti, Rochmah; Legowo, Djoko; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31135

Abstract

According to WHO (2010), the Trimeresurus albolabris snake is a venomous snake with a high number of bite cases in Indonesia. Information on the character and level of toxicity of snake venom in Indonesia, including Trimeresurus spp. snakes, is still minimal. This study aims to determine the LD50 value (lethal dose 50), clinical symptoms, and to determine the macroscopic appearance of the liver and kidneys of mice (Mus musculus) after being injected by a the white-lipped pit viper (Trimeresurus albolabris) venom. The LD50 value of Trimeresurus albolabris venom injected subcutaneously into mice was 5,09 mg/kgBW. Clinical symptoms that appear increased movement activity, increased respiratory rate, lethargy, decreased activity, and reduced grooming. Macroscopic appearance of the liver that looks abnormal is found (1) color changes in the form of pale, pale yellowish, and red spots (petechiae). (2) changes in surface structure found only one liver that is not flat. (3) changes in consistency in the form of brittle easily. In the kidney that looks abnormal, found (1) changes in color in the form of pale. (2) changes in surface structure were only found in one kidney in the form of nodules. (3) changes in consistency in the form of brittle easily and soft.
The Effect of Giving Gel Combination of Binahong Leaf Extract and Turmeric Rhizome Extract on Histopathological Epithelial Thickness in II B Degree Burn of Rattus norvegicus Indriyanti, Astrid; Rachmawati, Kadek; Maslachah, Lilik; Damayanti, Ratna; Hidayatik, Nanik; Setiawan, Boedi
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.45753

Abstract

This aim of this research was to study the effect of giving a combination gel of binahong (Anredera cordifolia) leaf and turmeric (Curcuma longa Linn) rhizome extract on histopathology of epithelial thickness which has II B degree burns. Twenty five male white rats (Rattus norvegicus) were divided into five groups. K(-) group was normal skin, K(+) group was skin burns treated with 1% silver sulfadiazine, P1, P2, and P3 was skin burns treated with gel combination of binahong leaf extract and turmeric rhizome extract with increase concentration of binahong leaf extract i.e. 1.25%, 2.5%, and 5%, while the concentration of turmeric rhizome extract i.e. 2% for each treatment. The combination gel of binahong leaf extrac 1.25% and turmeric rhizome extract 2% has potential to be an effective treatment for II B burns as indicated by increased of epithelial thickness. Therefore, this study concluded that 1.25% combination gel of binahong leaf extract and turmeric rhizome extract 2% is the effective dosage to increase epithelial thickness on II B healing degree burns wound healing.
Isolation, Identification and Risk Factors of Staphylococcus aureus Bacteria in Dairy Goat Milk with Subclinical Mastitis in Siliragung District, Banyuwangi Regency Faizah, Ayu Nur; Setiawan, Boedi; Saputro, Amung Logam; Warsito, Sunaryo Hadi; Praja, Ratih Novita; Fikri, Faisal
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.48012

Abstract

Mastitis is an inflammation of the mammary glands that can cause a sharp reduction in milk production and income for farmers. Subclinical mastitis is mastitis without clinical symptoms but can result in a decrease in the quantity and quality of milk production. One of the main pathogens that cause mastitis namely Staphyloccocus aureus. S. aureus become the biggest problem of mastitis because the cure rate is classified as very low. The risk factors for the occurrence of mastitis, both clinical and subclinical mastitis, can cause a decrease in milk production caused by several factors, including genetic, feed and environmental factors. This study aims to determine the presence of subclinical mastitis in milk of dairy goats with sufferers in Siliragung District, Banyuwangi Regency. Sample testing was carried out at the SIKIA Instrument Laboratory, Airlangga University. Isolation was carried out using MSA (Mannitol Salt Agar) media for further identification consisting of Gram staining and biochemical tests. Some of the biochemical tests used are the catalase test, the coagulase test and the VP test. The results of isolation, observation of characteristics and identification of bacteria in the research that has been carried out show that 64.51% (40/62) of the samples examined were positively identified S. aureus. Results showed that the risk factors for causing subclinical mastitis included cleaning the cage, the number of lactating goats, washing hands before milking, manure and waste management, weaning age and lactation age.
Isolation, Identification and Risk Factors of Escherichia coli Bacteria in Dairy Goat's Milk Subclinical Mastitis in Siliragung District, Banyuwangi Regency Lestari, Dewi Sri; Setiawan, Boedi; Saputro, Amung Logam; Mafruchati , Maslichah; Praja , Ratih Novita; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.49401

Abstract

Mastitis is an inflammation of the mammary gland which can cause a decrease in milk production and income for farmers. Subclinical mastitis is mastitis without clinical symptoms but can result in a decrease in the quality of milk production. One of the main pathogens that cause mastitis is Escherichia coli. The risk factors for the occurrence of mastitis, both clinical and subclinical mastitis, can cause a decrease in milk production caused by several factors, including genetic, feed, and environmental factors. This study aims to determine the presence of subclinical mastitis in dairy goat milk in Siliragung District, Banyuwangi Regency. Sample testing was carried out at the SIKIA Instrument Laboratory, Airlangga University. Isolation was carried out using EMBA media (Eosin Methylene Blue Agar) for further identification consisting of Gram staining and biochemical tests. One of the biochemical tests used is the IMViC (Indole Methyl red Voges-Proskauer Citrate Test). The results of isolation, observation of characteristics, and identification of bacteria in the research that was carried out showed that 8% (5/62) of the samples tested were positively identified as Escherichia coli. The results showed that the risk factors for causing subclinical mastitis included cleaning the cage bedding, washing hands before milking, milking out, teat dipping, and medication.
Occurrence of Anaplasma sp. infection in pigs (Sus scrofa) at Morgan Farm, Palangka Raya City Febriyatin, Fierdausi Nur’Aini; Setiawan, Boedi; Yuniarti, Wiwik Misaco; Wibawati, Prima Ayu; Yudhana, Aditya
Journal of Basic Medical Veterinary Vol. 13 No. 2 (2024): Journal of Basic Medical Veterinary, December 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i2.56944

Abstract

This research was conducted to identify the occurrence of Anaplasma sp. infection in pigs (Sus scrofa) at Morgan Farm, Pahandut Subdistrict, Palangka Raya City. Palangka Raya City is one of the cities that has great potential to develop pig livestock, but the shortcomings of the farms in Palangka Raya City still apply traditional housing with poor cage sanitation. Samples in this study were obtained from Morgan Farm with a total of 68 pig blood samples, then examined at the Animal Health and Veterinary Public Health Laboratory of Palangka Raya City, then starting with the preparation of PBS solution and making blood review preparations using 10% Giemsa staining. The blood obtained was then reviewed using object glass, absolute methanol, and stained with Giemsa 10%. Identification was carried out using a trinocular microscope with a magnification of 400x and immersion oil was dripped on the object glass. The results of this research showed that 29 pig blood samples were positive out of 68 pig samples, with an occurrence rate of 42.6%. The morphology of Anaplasma sp. found is that there is a round, purplish-blue dot shape at the edge of red blood cells. From these results it can be concluded that the infection of Anaplasma sp. in pigs (Sus scrofa) at Morgan Farm, Pahandut District, Palangka Raya City is 42.6%, which means that the infection is very high for Palangka Raya City.
Ethanolic extract of Dayak onion (Eleutherine palmifolia) prevented sperm membran damage in mice exposed to monosodium glutamate Syahraini Adhiya Lathifah; Aldin Akbar Rahmatullah; Boedi Setiawan; Chairul Anwar Nidom; Nove Hidajati; Sri Mulyati; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.153-160

Abstract

Monosodium glutamate (MSG) could cause increased production of reactive oxygen species (ROS), causing oxidative stress in the testicles, which adversely affected sperm quality. Dayak onion extract which is known for its high antioxidant content, could help alleviate oxidative stress caused by MSG. This research examined the effect of Dayak onion (Eleutherine palmifolia) on sperm abnormalities and plasma membrane integrity in mice (Mus musculus) exposed to MSG. Twenty-five male mice, 8 weeks old with a body weight approximately 20g, were divided into five groups, C– (received 0.5% Na-CMC), C+ (received 4 mg/g bw MSG), and three treatment groups (T1, T2, T3) which were given 4 mg/g bw MSG accompanied by Dayak onion extract at doses of 30, 60, and 120 mg/kg bw respectively. All treatments lasted for 52 days. Significant differences (p <0.05) in sperm abnormalities and plasma membrane integrity were observed among the groups. Sperm abnormalities found were (12.3 ± 1.92), (61.1 ± 3.10), (41.6 ± 2.87), (30.4± 1.91), and (18.2 ± 2.10) % respectively for C–, C+, T1, T2 and T3. Meanwhile, sperm plasma membrane integrity found were (33.0 ± 3.24), (69.3 ± 2.32), (41.8 ± 2.42), (55.4 ± 3.11), and (64.2 ± 1.27) % respectively for C–, C+, T1, T2 and T3. These results indicate that Dayak onion extract could help reduce sperm abnormalities and maintain plasma membrane integrity in mice exposed to MSG.
Evaluation and Its Impact of SARS-CoV-2 Inactivated Vaccine Candidate in K18-hACE2 Mice A'la, Rofiqul; Rantam, Fedik Abdul; Wijaya, Andi Yasmin; Susilowati, Helen; Kuncorojakti, Suryo; Diyantoro, Diyantoro; Aswin, Ahmad; Rahmahani, Jola; Suwanti, Lucia Tri; Lukiswanto, Bambang Sektiari; Yudaniayanti, Ira Sari; Setiawan, Boedi
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.54-62

Abstract

The COVID-19 pandemic caused by SARS-CoV-2 requires effective vaccines to be developed. This study aimed to assess the impact of a SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice by monitoring their body weight, immune activation, and inflammatory cytokines including IL-4, IL-6, TNF-α, and IFN-γ. The study utilized k18-hACE2 mice expressing the human angiotensin-converting enzyme 2 (hACE2) receptor. The mice were administered the inactivated vaccine candidate compared with sham and vehicle. Body weight was monitored, and serum samples were collected to measure IL-4, IL-6, TNF-α, and IFN-γ levels using ELISA. Data were evaluated using SPSS statistical analysis software. The administration of the SARS-CoV-2 inactivated vaccine candidate in k18-hACE2 mice did not result in significant changes in body weight compared to the control group. Furthermore, the levels of IL-4, IL-6, TNF-α, and IFN-γ were significantly reduced in the vaccinated mice compared to the control group, suggesting a dampening effect on the inflammatory response. This study demonstrates that the SARS-CoV-2 inactivated vaccine candidate has a minimal impact on the body weight of k18-hACE2 mice. Nevertheless, it successfully regulates the levels of IL-4, IL-6, TNF-α, and IFN-γ, suggesting its safety and beneficial impact. These findings contribute to understanding the vaccine's efficacy and safety profile in vaccine development.
Multi-Drug Resistant (MDR) Detection in Escherichia coli in Canary Birds (Serinus canaria) Imported from Malaysia Istiana, Izzatul; Effendi, Mustofa Helmi; Kurniawan, Muhammad ‘Ahdi; Sari, Fifin Kurnia; Purbowati, Tri Endah; Rahmandari, Dina Agylia; Mufasirin; Setiawan, Boedi; Rahardjo, Dadik
Media Kedokteran Hewan Vol. 36 No. 2 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i2.2025.123-136

Abstract

The frequency of canary imports continues to increase every year. Antibiotic resistance is a global problem that threatens human and animal health worldwide. Human interaction with birds as pets is a public health concern because it has the potential to increase zoonotic diseases. This study was conducted to identify the antibiotic resistance of E. coli using fecal swab samples of imported canaries from Malaysia. Samples were grown on an EMBA medium for the isolation test, Gram staining test, and IMViC test were performed to continue the identification test -Kirby-Bauer diffusion test - to determine antibiotic sensitivity. Based on morphological culture, Gram staining, and biochemical tests, the sample examination results showed 18% (27/150) were positive for E. coli, 16% (24/150) showed the highest resistance to tetracycline, 15,33% (23/150) amoxicillin, 12,66% (19/150) trimethoprim-sulfamethoxazole, 6% (9/150) ciprofloxacin, and 14,66% (22/150) isolates were confirmed MDR because they were resistant to three to four antibiotics. Further efforts are needed to understand and address the factors that lead to antibiotic resistance in the context of animal and public health. Prudent management of antibiotic use and monitoring of antibiotic resistance needs to be improved to maintain animal health and prevent the risk of transmission of resistant bacteria to humans.
Sex determination of Bangkok Zebra dove (Geopelia striata) based on wing and tail feather morphometrics at 3 and 6 months of age Kamilah Nurizza; Yeni Dhamayanti; Epy Muhammad Luqman; Gracia Angelina Hendarti; Soeharsono Soeharsono; Boedi Setiawan
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 2 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i2.2025.98-106

Abstract

This study aimed to determine the sex of Bangkok Zebra doves (Geopelia striata) based on external body morphometric measurements. A total of 32 doves were used, consisting of 16 females (8 bird each at 3 and 6 months of age) and 16 males (8 birds each at 3 and 6 months of age). Four morphometric parameters were measured: wing chord, antebrachium (forearm) length, longest primary feather, and longest tail feather. Wing and forearm lengths were measured using digital calipers with a precision of 0.01 mm, while the longest primary and tail feathers were measured using a ruler with 0.05 mm precision. The results showed that the average wing chord, longest primary feather, and longest tail feather lengths were significantly greater (p <0.05) in males compared to females. At six months of age, males also had a significantly longer forearm than females (p <0.05). However, no significant difference (p >0.05) was found in forearm length between sexes at three months of age. It could be concluded that wing and tail feather morphometrics can be used to distinguish between male and female Bangkok Zebra doves at both three and six months of age, except for forearm length at three months.
Co-Authors A'la, Rofiqul Abdul Samik, Abdul Agus Widodo Agus Wijaya Agustono, Bodhi Ahmad Herdianto Wicaksono Aksono HP., Eduardus Bimo Aldin Akbar Rahmatullah Alfiana Laili Dwi Agustin Alfina Azkiana Ana Rohmatul Laili Aprinda Ratna Lovela Arimbi Arum Puspitasari Aswin, Ahmad Azami D Azinar Azizah Bilqis Nurkarimah Bambang Poernomo Bambang Sektiari Lukiswanto Benny Perdana Budi I Santoso Budi Santoso Budi Utomo Chusnannada, Nabila Dadik Rahardjo Dadik Rahardjo, Dadik Daniel Leonardo Darmawan, Moch. Rizky Dellavia, Yolanda Dina Agylia Rahmandari Diyantoro Diyantoro, Diyantoro Djoko Legowo Djoko Legowo Doohan Mahendra Edward Yonas Kristijanto Eighty M Kurniawati Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Eka Pramyrtha Hestinah Emmanuel Djoko Poetranto Endang Suprihati Epy Muhammad Luqman Erma Safitri Faisal Fikri Faizah, Ayu Nur Febriyatin, Fierdausi Nur’Aini Fedik A Rantam Fedik Abdul Rantam Fianti Nufinda Rachma Fifin Kurnia Sari Gatut Hardianto, Gatut Ghifari Lutfi Fauzi Hani Plumeriastuti Hari Paraton Hartiningsih - Helen Susilowati, Helen Hendarti, Gracia Angelina Heni Puspitasari Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Indasari, Elly Nur Indiastuti, Danti Nur Indriyanti, Astrid Ira Sari Yudaniayanti Ismudiono Ismudiono Ismudiono, Ismudiono Istiana, Izzatul Iwan Sahrial Hamid Izzatul Istiana Kadek Rachmawati Kamilah Nurizza Kartika Aditiya Amelia Khairunnisa, Anindita Koesdarto , Setiawan Kuncoro Puguh Santoso Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lastuti , Nunuk Dyah Retno Legowo, Djoko Lestari, Dewi Sri Lilik Maslachah Lilik Maslachah LINA SUSANTI Lita Rakhma Yustinasari Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Melda Mufidatul Ifqiyyah Mochamad Lazuardi Mohamad Safri Sauqi Mufasirin Mustofa Helmi Effendi Mustofa Helmi Effendi Nidom, Chairul Anwar Niken Hastuti, Apriliwiyani Nove Hidajati Nur AR Widiatmoko Nusdianto Triakoso Pinayungan, Probo Probo Warih Tatag Poedji Hastutiek Praja, Ratih Novita Prastika, Dinda Dwi Prastiya, Ragil Angga Pratama, Ponasari Galuh Primarizky, Hardany Pudji Srianto Puput Ade Wahyuningtyas Purbowati, Tri Endah Rachmanita, Rona Taufiqul Rahaju Ernawati Rahmahani, Jola Rahmandari, Dina Agylia Rahmi Sugihartuti Ramadhanty, Miladhiyah Nabila Ranto Bayu Damanik Ratna Damayanti Ratna Damayanti Ratna Damayanti Retno Wulansari Rochmah Kurnijasanti Rury Mega Wahyuni Saputro, Amung Logam Sari, Fifin Kurnia Sesa Puput Febriyanti Setiawan Koesdarto Soeharsono Soeharsono Soeharsono Soeharsono Sofyan, Miyayu Soneta Sri Hidanah Sri Mulyati Srimaryanto, Leonardo Reza Sudarminto - Sudjono, Budi Santoso Sugihartuti , Rahmi Sunarso, Agus Syahraini Adhiya Lathifah Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Endah Purbowati Tri HS Hadi Tri Lestari Tri Wahyu Suprayogi Triagil, Annisa Lita Tyasningsih, Wiwiek Tyasningsih, Wiwiek Vivi Oktaviana Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Wijaya, Andi Yasmin Wiwiek Tyasningsih Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Yeni Dhamayanti Yonatan Dimascahyo Budianto Yudhana, Aditya Yulianna Puspitasari Yulianto, Rangga