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All Journal Majalah Kedokteran Bandung Media Kedokteran Hewan Majalah Obstetri dan Ginekologi Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Veterinary Biomedical and Clinical Journal Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences.
Boedi Setiawan
Departemen Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga Kampus C Unair, Jl. Mulyorejo, Kota Surabaya, Jawa Timur, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Veterinary

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The Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction Pinayungan, Probo Probo Warih Tatag; Aprinda Ratna Lovela; Eduardus Bimo Aksono; Ismudiono; Boedi Setiawan; Kadek Rachmawati; Jola Rahmahani
Media Kedokteran Hewan Vol. 35 No. 1 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i1.2024.21-27

Abstract

Sex identification of Sun Conures (Aratinga solstitialis) is crucial for breeding and preservation, as well as increasing sun conure populations. These birds are sexually monomorphic. Therefore, Determination between male and female carried out by their morphology examination. The Polymerase Chain Reaction (PCR) method, utilizing molecular-based technology, was employed to determine the sex of Aratinga solstitialis in this study. The P2 and P8 primers were utilized in this method, which has been deemed suitable and accurate for sex identification through calamus samples. The research focused on two 28-month-old Aratinga solstitialis birds. Calamus samples were collected and subjected to PCR amplification using the extracted calamus. The resulting PCR products were then visualized using electrophoresis with a 1% agarose gel. In the electrophoresis photo, the presence of two bands indicated a female specimen, whereas a single band indicated a male specimen. The result of the gel electrophoresis research showed that both of the Aratinga solstitialis were male with one band of each bird on ranged from 300-400 base pairs. The result show that the Polymerase Chain Reaction method in terms for sex identification on monomorphic birds, especially Aratinga solstitialis birds is very effective to differentiate the sex of young birds and the adults.
Detection of Cryptosporidium spp. in Wild Rats (Rattus spp.) in Surabaya, East Java Pratama, Ponasari Galuh; Suprihati, Endang; Suwanti, Lucia Tri; Mufasirin; Hastutiek, Poedji; Setiawan, Boedi; Puspitasari, Heni; Indasari, Elly Nur
Media Kedokteran Hewan Vol. 35 No. 2 (2024): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v35i2.2024.71-78

Abstract

Cryptosporidiosis is a waterborne zoonosis caused by Cryptosporidium, which is a parasite that causes infection of the small intestine and leads to acute diarrhea in both humans and animals. Wild rats (Rattus spp.) are known to carry many zoonotic pathogens including Cryptosporidium. Given their close proximity to humans in urban environments, the likelihood of disease transmission is high. This study aims to detect the Cryptosporidium spp. infection in wild rats in Surabaya City, East Java Province. Intestinal fecal samples were collected using necropsy. The flotation test and Ziehl-Neelsen stain were used to observe the presence of Cryptosporidium under the microscope. The results showed that 69 samples were positive for Cryptosporidium spp. in the oocyst stage, which appeared dark pink with a clear cavity and the size of the oocysts ranged from 2-6 μm. The high incidence of cryptosporidiosis in wild rats is a potential health threat to both animals and humans.
Relationship between the value of the estrous detector measurement result and serum progesterone level in Etawa crossbred goats after estrous synchronization Triagil, Annisa Lita; Ismudiono, Ismudiono; Lestari, Tita Damayanti; Samik, Abdul; Utomo, Budi; Setiawan, Boedi
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.82-85

Abstract

This study was conducted to determine the relationship between the estrous detector's reading value (Draminski®, DramiÅ„ski S.A, Poland) and serum progesterone levels on Etawa crossbred does. Eighteen healthynon-prehnant Etawa crossbreds does weighing 20-25 kg, in their first parity were used in this study. The does were divided equally into two groups. In the two groups, the does were synchronized their estrus by means of intramuscular and intravulvar injection of 7.5 mg prostaglandin FÉ‘ (PGF2É‘) twice in 11-day intervals. After the second injection, the estrous detector value measurement, visual observation of the estrus sign, and blood sampling were conducted for five days. The blood sample was processed into the serum to examine for progesterone levels by ELISA. The data analysis was using the simple correlation method on SPSS type 23 for windows. The result showed that a positive relationship (p <0.05) with coefficient correlation (r) was 0.609 between the values of the estrous detector measurement with serum progesterone levels. It could be concluded that the estrous detector's low value indicates that the Etawa crossbred was in the estrus phase and followed by the decreasing of progesterone level but not reaching the basal level.
Determination of sexual maturity of Indonesian box turtle (Cuora amboinensis couro) based on straight carapace length Daniel Leonardo; Pudji Srianto; Iwan Sahrial Hamid; Boedi Setiawan; Djoko Legowo; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.115-122

Abstract

Predicting the turtle's reproductive status and sexual maturity is necessary for ex-situ breeding and conservation program. The histology of the seminiferous tubules of the Indonesian box turtle (Cuora amboinensis couro) at various ages has not been studied much. This study aims to develop equations to predict the sexual maturity of Indonesian box turtles based on straight carapace length (SCL). Six turtles with SCL sizes 10-21 cm were sacrificed and their testes were taken for morphometric measurements and histological preparations for Hematoxylin Eosin staining. Pearson's correlation of SCL with testicular maturity parameters was analyzed, followed by Anova Regression. The results showed that there were differences in the morphometry and topographic anatomy of the Indonesian box turtle testes between SCL below 15 cm and above 15 cm. Six turtles with SCL size of 10-21 cm were sacrificed and their testes were taken for morphometric measurements and histological preparations for Hematoxylin Eosin staining. Pearson's correlation of SCL with testicular maturity parameters was analyzed, followed by Regression Anova. The results showed that there were differences in the morphometry and topographic anatomy of the Indonesian box turtle testes between SCL below 15 cm and above 15 cm.
Penilaian densitas kolagen pada luka bakar derajat II tikus putih (Rattus norvegicus) yang diterapi dengan krim ekstrak daun melinjo (Gnetum gnemon L.) Srimaryanto, Leonardo Reza; Hidajati , Nove; Triakoso , Nusdianto; Herupradoto, Eduardus Bimo Aksono; Lazuardi, Mochamad; Setiawan, Boedi
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 1 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i1.680

Abstract

Second-degree burns are complex traumatic conditions requiring optimal tissue healing interventions. This experimental study investigated the impact of Gnetum gnemon L. leaf extract cream on collagen density in burn wounds. The research was conducted using 20 male Wistar rats (Rattus norvegicus), divided into five groups: negative control, positive control (silver sulfadiazine), and three treatment groups with varying leaf extract concentrations (2.5%, 5%, and 10%). Burn induction was performed on the right gluteal area using a thermostat at 85°C for 5 seconds. Collagen density was evaluated through histopathological analysis using ImageJ with density scores ranging from 0 to 4. Research results demonstrated significant collagen density increases in treatment groups, with the 10% concentration displaying the highest density (score +4). Significant differences between groups were confirmed by statistical analysis (p<0.05). Findings indicate the potential of Gnetum gnemon L. extract as an alternative therapy for improving burn wound healing through collagen density optimization.
Environmental and Management Risk Factors for Goat Mastitis in Banyuwangi Lestari, Dewi Sri; Setiawan, Boedi; Saputro, Amung Logam; Mafruchati, Maslichah; Praja, Ratih Novita; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.49399

Abstract

Mastitis is a persistent and economically significant health problem in dairy goats in Banyuwangi, East Java, where smallholder production systems dominate. This review synthesises current evidence on the primary risk factors contributing to the high prevalence of subclinical mastitis in the region. Local studies consistently report Staphylococcus aureus as the leading etiological agent, frequently exhibiting antimicrobial resistance linked to unsupervised antibiotic use. Host-related factors such as advanced parity, udder lesions, and teat-end damage increase susceptibility to intramammary infection. Management practices—including inadequate milking hygiene, use of shared towels, absence of pre- and post-dipping, and irregular equipment sanitation—facilitate the transmission of contagious pathogens. Environmental conditions, particularly high humidity and poor housing sanitation, further elevate infection pressure. Socio-economic constraints and limited farmer knowledge about subclinical mastitis and antibiotic stewardship exacerbate disease persistence. The multifactorial nature of mastitis in Banyuwangi underscores the need for context-appropriate interventions combining improved hygiene practices, better housing management, routine screening, and responsible antimicrobial use. Strengthened farmer education and targeted surveillance are essential to reduce mastitis prevalence and enhance the sustainability of goat dairy production in the region.
Determination of puberty based on morphometric measurements and the gonadosomatic index of lovebird (Agapornis fischeri) testes Chusnannada, Nabila; Yeni Dhamayanti; Boedi Setiawan; Sunaryo Hadi Warsito; Tri Wahyu Suprayogi; Kuncoro Puguh Santoso
Ovozoa: Journal of Animal Reproduction Vol. 15 No. 1 (2026): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v15i1.2026.34-43

Abstract

This study aimed to determine the relationship between age and testicular morphometrics in lovebird (Agapornis fischeri), as well as the relationship between age and the gonadosomatic index (GSI) of the testes, and to evaluate the effect of body weight on testicular weight in determining the onset of puberty. This study used 30 lovebirds divided into three age groups: 4, 5, and 6 months, with ten birds in each group. The variables measured were testicular length, width, volume, weight and body weight. Data were analyzed using correlation analysis, simple linier regression, and paired sample t-test. The results showed a positive relationship between age and testicular length, width and volume. The GSI values of lovebirds aged 4, 5 and 6 months were 0.015 ± 0.002, 0.058 ± 0.011, and 0.400 ± 0.067, respectively, indicating an increase with age. The significance coefficient at 4 months of age was 0.000 (p <0.05), indicating that body weight had a significant influence on testicular weight at this age. In contrast, lovebirds aged 5 and 6 months had significance coefficients of 0.079 and 0.089 (p >0.05), indicating that testicular weight was not significantly influenced by body weight at these ages. It can be concluded that lovebirds at 6 months of age are likely to have reached puberty.
Detection of ESBL-Producing Escherichia coli in Dogs Transported Through Juanda International Airport Quarantine, East Java, Indonesia Benny Perdana; Mustofa Helmi Effendi; Wiwiek Tyasningsih; Wiwik Misaco Yuniarti; Boedi Setiawan; Dadik Rahardjo
Media Kedokteran Hewan Vol. 37 No. 2 (2026): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v37i2.2026.194-203

Abstract

Antibiotic resistance poses a serious threat to global health, affecting both human and animal health sectors. Antibiotic resistance can be found in both healthy and diseased animals and may also be detected in their owners. Close interaction between humans and their pets, particularly dogs, can facilitate the transmission of antibiotic-resistant bacteria. Extended-spectrum beta-lactamase (ESBL) is one of the mechanisms of antibiotic resistance. Escherichia coli is an ESBL-producing bacterium that inhabits the gastrointestinal tract of living organisms, including humans and dogs. Infections caused by E. coli may lead to diseases, such as urinary tract infections. Infections involving antibiotic-resistant E. coli result in more difficult and prolonged treatment. This study aimed to detect ESBL-producing E. coli in dogs transported through Juanda International Airport. Rectal swabs were collected as samples. Bacterial identification was performed using EMB agar, Gram staining, and biochemical tests. Confirmation of ESBL-producing E. coli was performed using the double-disk synergy test (DDST). The results showed that 75.22% (85/113) were positive for E. coli, with 2.6% (3/113) confirmed as ESBL-producing by DDST.
A comperhensive review: Antimicrobial Resistance in Indonesia One Health Challenge Ranto Bayu Damanik; Alfiana Laili Dwi Agustin; Agus Widodo; Eka Pramyrtha Hestianah; Danti Nur Indiastuti; Ratna Damayanti; Kuncoro Puguh Santoso; Boedi Setiawan; Rahmi Sugihartuti; Vivi Oktaviana; Rury Mega Wahyuni; Lita Rakhma Yustinasari
VITEK : Bidang Kedokteran Hewan Vol. 16 No. 1 (2026): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/3svevz51

Abstract

Antimicrobial resistance has become a major global health concern affecting humans, animals, and the environment. The increasing occurrence of antibiotic resistant bacteria threatens the effectiveness of antimicrobial therapy and poses serious public health risks. This review aims to describe the occurrence and distribution of antimicrobial resistant Escherichia coli across humans, animals, wildlife, and environmental sources using a One Health perspective. A literature review approach was conducted by collecting scientific articles published between 2015 and 2025 from international and national databases including PubMed, Scopus, and Google Scholar. Articles were selected based on relevance to antimicrobial resistance in Escherichia coli within human, animal, and environmental contexts. The reviewed studies indicate that Escherichia coli isolates frequently show resistance to commonly used antibiotics such as ampicillin, tetracycline, fluoroquinolones, and third generation cephalosporins. Multidrug resistant strains and extended spectrum beta lactamase producing bacteria have been reported in clinical samples, livestock, companion animals, wildlife, and environmental samples including wastewater and river water. These findings highlight the interconnected nature of antimicrobial resistance transmission between humans, animals, and the environment. Integrated surveillance and responsible antibiotic use are essential to control the spread of antimicrobial resistance through the One Health approach
Detection of Vancomycin-Resistant Enterococci (VRE) Genes in Imported Canary (Serinus canaria) Faeces Dina Agylia Rahmandari; Mustofa Helmi Effendi; Yulianna Puspitasari; Eduardus Bimo Aksono Herupradoto; Lilik Maslachah; Boedi Setiawan; Fifin Kurnia Sari; Tri Endah Purbowati; Izzatul Istiana
Journal of Basic Medical Veterinary Vol. 15 No. 1 (2026): Journal of Basic Medical Veterinary, June 2026
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v15i1.83728

Abstract

Antibiotic-resistant Enterococcus is currently one of the leading causes of nosocomial infections worldwide. Multidrug-resistant (MDR) Enterococcus is an important nosocomial pathogen and represents a growing clinical challenge. This study aimed to determine the presence and characteristics of Vancomycin-Resistant Enterococci (VRE) genes in the faeces of imported canaries (Serinus canaria) to assess their potential impact on animal health and the spread of antimicrobial resistance. A total of 150 canary faecal samples were randomly collected from a single importer. Colonies with Enterococcus-like morphology were identified at the genus level based on culture characteristics, Gram staining, catalase testing, and bile-esculin testing. Species identification was confirmed through carbohydrate fermentation tests. Antibiotic susceptibility testing was performed using the Kirby–Bauer method on Mueller–Hinton agar. Polymerase chain reaction (PCR) testing was subsequently conducted to identify the vanB gene. Among the samples, 39 (26%) were positive for Enterococcus faecalis. Of these isolates, 7 (17.9%) were identified as multidrug-resistant (MDR), and 5 (71.4% of MDR isolates) were classified as VRE. One of the five VRE isolates (20%) was found to carry the vanB gene, an important determinant of vancomycin resistance. These findings indicate that imported canaries may serve as reservoirs of antimicrobial-resistant bacteria with the potential to spread to the environment, other animals, and humans through the faecal–oral route or environmental contamination.
Co-Authors A'la, Rofiqul Abdul Samik, Abdul Agus Widodo Agus Wijaya Agustono, Bodhi Ahmad Herdianto Wicaksono Aksono HP., Eduardus Bimo Aldin Akbar Rahmatullah Alfiana Laili Dwi Agustin Alfina Azkiana Ana Rohmatul Laili Aprinda Ratna Lovela Arimbi Arum Puspitasari Aswin, Ahmad Azami D Azinar Azizah Bilqis Nurkarimah Bambang Poernomo Bambang Sektiari Lukiswanto Benny Perdana Budi I Santoso Budi Santoso Budi Utomo Chusnannada, Nabila Dadik Rahardjo Dadik Rahardjo, Dadik Daniel Leonardo Darmawan, Moch. Rizky Dellavia, Yolanda Dina Agylia Rahmandari Diyantoro Diyantoro, Diyantoro Djoko Legowo Djoko Legowo Doohan Mahendra Edward Yonas Kristijanto Eighty M Kurniawati Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Eka Pramyrtha Hestinah Emmanuel Djoko Poetranto Endang Suprihati Epy Muhammad Luqman Erma Safitri Faisal Fikri Faizah, Ayu Nur Febriyatin, Fierdausi Nur’Aini Fedik A Rantam Fedik Abdul Rantam Fianti Nufinda Rachma Fifin Kurnia Sari Gatut Hardianto, Gatut Ghifari Lutfi Fauzi Hani Plumeriastuti Hari Paraton Hartiningsih - Helen Susilowati, Helen Hendarti, Gracia Angelina Heni Puspitasari Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Indasari, Elly Nur Indiastuti, Danti Nur Indriyanti, Astrid Ira Sari Yudaniayanti Ismudiono Ismudiono Ismudiono, Ismudiono Istiana, Izzatul Iwan Sahrial Hamid Izzatul Istiana Kadek Rachmawati Kamilah Nurizza Kartika Aditiya Amelia Khairunnisa, Anindita Koesdarto , Setiawan Kuncoro Puguh Santoso Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lastuti , Nunuk Dyah Retno Legowo, Djoko Lestari, Dewi Sri Lilik Maslachah Lilik Maslachah LINA SUSANTI Lita Rakhma Yustinasari Lucia Tri Suwanti, Lucia Tri Mafruchati, Maslichah Melda Mufidatul Ifqiyyah Mochamad Lazuardi Mohamad Safri Sauqi Mufasirin Mustofa Helmi Effendi Mustofa Helmi Effendi Nidom, Chairul Anwar Niken Hastuti, Apriliwiyani Nove Hidajati Nur AR Widiatmoko Nusdianto Triakoso Pinayungan, Probo Probo Warih Tatag Poedji Hastutiek Praja, Ratih Novita Prastika, Dinda Dwi Prastiya, Ragil Angga Pratama, Ponasari Galuh Primarizky, Hardany Pudji Srianto Puput Ade Wahyuningtyas Purbowati, Tri Endah Rachmanita, Rona Taufiqul Rahaju Ernawati Rahmahani, Jola Rahmandari, Dina Agylia Rahmi Sugihartuti Ramadhanty, Miladhiyah Nabila Ranto Bayu Damanik Ratna Damayanti Ratna Damayanti Ratna Damayanti Retno Wulansari Rochmah Kurnijasanti Rury Mega Wahyuni Saputro, Amung Logam Sari, Fifin Kurnia Sesa Puput Febriyanti Setiawan Koesdarto Soeharsono Soeharsono Soeharsono Soeharsono Sofyan, Miyayu Soneta Sri Hidanah Sri Mulyati Srimaryanto, Leonardo Reza Sudarminto - Sudjono, Budi Santoso Sugihartuti , Rahmi Sunarso, Agus Syahraini Adhiya Lathifah Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Endah Purbowati Tri HS Hadi Tri Lestari Tri Wahyu Suprayogi Triagil, Annisa Lita Tyasningsih, Wiwiek Tyasningsih, Wiwiek Vivi Oktaviana Wardhana, Dhandy Koesoemo Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Wijaya, Andi Yasmin Wiwiek Tyasningsih Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Wiwik Misaco Yuniarti Yeni Dhamayanti Yonatan Dimascahyo Budianto Yudhana, Aditya Yulianna Puspitasari Yulianto, Rangga