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All Journal Media Kedokteran Hewan Jurnal Medik Veteriner Veterinary Biomedical and Clinical Journal Jurnal Layanan Masyarakat (Journal of Public Service) Journal of Parasite Science Jurnal Abdidas Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Pharmaceutical and Sciences. Jurnal Agro Veteriner
Nove Hidajati
Departemen Ilmu Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya, Indonesia.
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Identification of Cat (Felis catus) Blood Splatter on Cotton Fabric After Periods of Drying Using Leucomalachite Green and Takayama Reagent Charles, Vanessa Ann; Lestari, Tita Damayanti; Legowo, Djoko; Ismudiono, Ismudiono; Hidajati, Nove; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28593

Abstract

Blood-stain or blood splatter analysis when used properly can assist in establishing a chain of events linked to violent crimes (Bevel and Gardner, 2008). The methods used in detecting blood splatters in the field are chemical methods. Leucomalachite green is a colorimetric test which is used to test the presence of blood (Castro and Coyle, 2008). Takayama reagent is a confirmatory test for blood (Strassman, 1922). The aim of this research is to detect the blood splatter on cotton fabric after it has been dried for 1 day, 3 days and 5 days using Leucomalachite green and Takayama reagent. Cotton fabric was specifically chosen for this experiment with 3 different periods of drying. The unstained cotton fabric was cut into squares, and a blood sample was splattered on each piece. The fabrics splattered with blood were then dried for 1 day, 3 days and 5 days. The blood splatter was then tested using Leucomalachite green and Takayama reagent, and the results were noted afterwards. For the control, red food dye was dried for 1 day then tested with Leucomalachite green and Takayama reagent. The image results of the Leucomalachite green test are analyzed using ImageJ software 1.8.0_112 where the red, green and blue pixels are converted to grayscale. The image results of the Takayama test are graded based on the number and pattern of crystal. In conclusion, Leucomalachite green and Takayama reagent are able to detect cat blood splatter on the cotton fabric. Leucomalachite green produced a higher intensity/ darker colour as a result of an older sample, and the lower intensity/ lighter colour as a result of a fresher sample of the Leucomalachite green test. Takayama reagent produced a densely packed pattern of crystals as a result of an older sample, and the loosely packed pattern of crystals as a result of a fresher sample of the Takayama test.
Toxicity Testing Of White Pomegranate (Punica granatum L.) Fruit Extracts Using Brine Shrimp Lethality Test Method As A Candidate Of Anti-Cancer Drug Firnanda, Ferian; Herupradoto, Eduardus Bimo Aksono; Rahmawati, Kadek; Kurnijasanti, Rochmah; Sukmanadi, Mohammad; Hidajati, Nove
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31810

Abstract

This research aimed to determine the effects of toxic white pomegranate fruit extract (Punica granatum L) against larvae of brine shrimp (Artemia salina Leach) indicated LC50 values below 1000 µg/ml. This study is purely experimental by using Brine Shrimp Lethality Test (BSLT). The study was divided into seven groups, namely ethanol extract of white pomegranate fruit (Punica granatum L) with a concentration of 31,25; 62,5; 125; 250; 500; 1000 µg/ml and negative control (seawater). Mortality data percentage of Artemia salina Leach analyzed by probit analysis. Results showed that the extract of white pomegranate fruit extract (Punica Granatum L) has a toxic effect with LC50 values of 248,6 µg/ml calculate from probit analysis. From these results, it can conclude that white pomegranate extract is toxic to larval shrimp (Artemia salina Leach) with Brine Shrimp Lethality Test (BSLT) method which means white pomegranate extract has the potential to be an anticancer drug.
The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

Abstract

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.
Effect of methanol extract of cloves (Syzigium aromaticum) on Protein Gene Product 9.5 in the testes of rats (Rattus norvegicus) with induced cryptorchidism Nufus, Faizah Zakiyyatun; Bambang Sektiari Lukiswanto; Ira Sari Yudaniyanti; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Budi Utomo
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.82-89

Abstract

This study aimed to know the effect of cloves (Syzigium aromaticum) methanol extracts as an antioxidant on the germ cells of white rats (Rattus norvegicus) with induced cryptorchidism. The subjects of this study were 24 male white rats aged 21 days, who were divided into six groups. Rats were adapted for 5 days, after which cryptorchidism were induced. Cloves extract was given for 18 days and 36 days. On day-19 and day-37, rats were sacrificed and testicles were taken for Protein Gene Product (PGP) 9.5 detection by immunohistochemistry. These groups consisted of negative control group (given distilled water and sham surgery), positive control group (induction of cryptorchidism and given distilled water), and T treatment group (induction of cryptorchidism and given 70mg/kg bw cloves extract. Kruskal-Wallis test indicated a significant difference (p <0.05). The differences between treatments were shown based on the outcome of the Mann-Whitney test. Cloves acted as an antioxidant for cryptorchid testicular germ cells. It could be concluded that administering methanol extract of clove flowers could ameliorate the expression of PGP 9.5 in the testicular germ cells of white rats in a model of unilateral cryptorchidism.
Black Soldier Fly (Hermetia illucens) Maggot Flour as Concentrate Substitution on Broiler Chickens Feed Consumption, Body Weight Gain and Feed Conversion Ratio Magdalena Eki Pertiwi; Sri Hidanah; Nove Hidajati; Mirni Lamid; Widya Paramita Lokapirnasari; Sunaryo Hadi Warsito
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51502

Abstract

This study aims to determine the effect of concentrate substitution with maggot Black Soldier Fly (Hermetia illucens) flour on feed consumption, body weight gain and feed conversion ratio of broiler chickens. This study was an experimental study with a completely randomized design (CRD) with 5 treatment and 4 replications. The material uses broiler chickens age four weeks as many as 20 tails, the feed used is concentrate from the PT. Charoen Pokphand Indonesia, maggot BSF flour, corn and bran. The feed treatment with the addition of maggot BSF flour consisted of P0 (feed without maggot BSF flour), P1 (2% addition of maggot BSF flour), P2 (4% addition of maggot BSF flour), P3 (6% addition of maggot BSF flour), and P4 (8% addition of maggot BSF flour). From the study results obtained the mean and standard deviation of feed consumption, body weight gain and feed conversion of broiler chickens. Analisys of Variance (ANOVA) showed that the subtitution of concentrate with maggot flour from black soldier flies had no significated effect (p>0.05) on the feed consumption, body weight gain and feed conversion ratio of broiler chickens.
The usage of cattle rumen contents in commercial quail feed towards the production and haugh unit (HU) of eggs (Coturnix coturnix japonica) Joel Jeevan Raj S/O Yogarajah; Tita Damayanti Lestari; Mohammad Anam Al Arif; Widya Paramita Lokapirnasari; Sri Hidanah; Nove Hidajati
Jurnal Agro Veteriner Vol. 8 No. 1 (2024): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i1.64989

Abstract

The purpose of this study was to determine the effect of rumen content fermentation on quail bird ration. The parameters measured were the production of egg weight and haugh unit. In total 24 quail birds were used with 4 treatments and 6 repetitions. Treatments were consists of T0(formulated feed without fermented rumen content), T1(formulated feed with 5% fermented rumen content), T2(formulated feed with 10% fermented rumen content), T3(formulated feed with 15% fermented rumen content). Samples are observed on the last day to determine the egg quality with the egg weight and haugh unit. The results showed that there are significant differences effected on the egg weight and haugh unit.
Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen Aditya Irawan; Fajar Ramadhan Subiyantoro; Suherni Susilowati; Tri Wahyu Suprayogi; Sri Pantja Madyawati; Pudji Srianto; Nove Hidajati; Eduardus Bimo Aksono Herupradoto; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.161-170

Abstract

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.
Ethanolic extract of Dayak onion (Eleutherine palmifolia) prevented sperm membran damage in mice exposed to monosodium glutamate Syahraini Adhiya Lathifah; Aldin Akbar Rahmatullah; Boedi Setiawan; Chairul Anwar Nidom; Nove Hidajati; Sri Mulyati; Tri Wahyu Suprayogi
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.153-160

Abstract

Monosodium glutamate (MSG) could cause increased production of reactive oxygen species (ROS), causing oxidative stress in the testicles, which adversely affected sperm quality. Dayak onion extract which is known for its high antioxidant content, could help alleviate oxidative stress caused by MSG. This research examined the effect of Dayak onion (Eleutherine palmifolia) on sperm abnormalities and plasma membrane integrity in mice (Mus musculus) exposed to MSG. Twenty-five male mice, 8 weeks old with a body weight approximately 20g, were divided into five groups, C– (received 0.5% Na-CMC), C+ (received 4 mg/g bw MSG), and three treatment groups (T1, T2, T3) which were given 4 mg/g bw MSG accompanied by Dayak onion extract at doses of 30, 60, and 120 mg/kg bw respectively. All treatments lasted for 52 days. Significant differences (p <0.05) in sperm abnormalities and plasma membrane integrity were observed among the groups. Sperm abnormalities found were (12.3 ± 1.92), (61.1 ± 3.10), (41.6 ± 2.87), (30.4± 1.91), and (18.2 ± 2.10) % respectively for C–, C+, T1, T2 and T3. Meanwhile, sperm plasma membrane integrity found were (33.0 ± 3.24), (69.3 ± 2.32), (41.8 ± 2.42), (55.4 ± 3.11), and (64.2 ± 1.27) % respectively for C–, C+, T1, T2 and T3. These results indicate that Dayak onion extract could help reduce sperm abnormalities and maintain plasma membrane integrity in mice exposed to MSG.
The Effect of Kemangi Leaf (Ocimum Basilicum) Extract in The Egg Yolk Skim Milk Diluent on The Plasma Membrane and Motility of Boerawa Goats' Sperm Farah Ghifara Hafidha; Utomo, Budi; Soeharsono; Lestari, Tita Damayanti; Hidajati, Nove; Hermadi, Herry Agoes
Media Kedokteran Hewan Vol. 36 No. 3 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i3.2025.273-281

Abstract

The purpose of this research was to determine the effect of kemangi leaf (Ocimum basilicum) extract in the egg yolk skim milk diluent on the plasma membrane and motility of Boerawa goats' sperm at room temperature. The research used fresh samples of Boerawa goat semen collected by artificial vagina. The experimental design used was a completely randomized design, divided into four treatments with six replications. The treatments were the addition of kemangi leaf extract at the levels of 0% (P0), 1% (P1), 2% (P2), and 3% (P3). Diluted semen was stored at room temperature and observed at hours 0, 1, 2, and 3. Analysis of the data using Multivariate Analysis of Variance (MANOVA), then proceed to the Duncan Test to determine significant differences between treatments. The results showed that there was no significant difference (p > 0.05) in sperm plasma membrane, but a significant difference (p < 0.05) in sperm motility was observed when kemangi leaf extract was added. However, the addition of kemangi leaf (Ocimum basilicum) extract tends to provide a better value than without it. The addition of kemangi leaf extract at a concentration of 2% yields the highest results in sperm plasma membrane integrity and sperm motility of Boerawa semen at room temperature.
PENGARUH PEMBERIAN EKSTRAK KULIT SEMANGKA (Citrullus lanatus) TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA TIKUS (Rattus norvegicus) DENGAN PAPARAN SUHU PANAS Rohmah, Lailatul; Triana, Indah Norma; Sunarso, Agus; Susilowati, Suherni; Hidajati, Nove; Kurnijasanti, Rochmah
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.302 KB) | DOI: 10.20473/ovz.v7i2.2018.131-136

Abstract

High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Watermelon's rind have an ability for antioxidants effect that can reduce sperm's damages that results by role explanation of free radicals. This study aimed to determine the effect of extract of watermelon's rind on the motility and viability of sperm on male rat exposed to heat. This experiments use rat as animals experiment with five treatments and four repetitions. Five treatments are: P0: without exposured to 40ËšC of temperature and without treatment by extract watermelon's rind, P1: was exposured to 40ËšC of temperature and without treatment by extract watermelon's rind, P2: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 20 mg/rat/day, P3: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 40 mg/rat/day, and P4: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 80 mg/rat/day. The treatment was done for 52 days. The results, P4 have the best effect that can be increase the motility and viability of rat's sperm (Rattus norvegicus) exposed to heat.
Co-Authors Abdul Samik, Abdul Aditya Irawan Agung Budianto Achmad, Agung Budianto Ahmad Fahrisa Sidharta Aksono HP., Eduardus Bimo Al arif, Mohammad Anam Al-Zuhroh, Tisya Yumn Aldin Akbar Rahmatullah Amaris, Aulia Puspa Ananda, Hanifah Muslimah Annisa Nur Fitria Rahma Anwar Ma'ruf Bambang Sektiari Lukiswanto Boedi Setiawan Budi Utomo Budi Utomo BUDI UTOMO Cahyono, Shahani Azpriyanne Chairul Anwar Charles, Vanessa Ann Chrismanto, Dony Dian Yulie Reindrawati Eka Pramyrtha Hestianah Eka Pramyrtha Hestianah, Eka Pramyrtha Emy Koestanti Sabdoningrum Faisal Fikri Fajar Ramadhan Subiyantoro Farah Ghifara Hafidha Firnanda, Ferian Gandul Atik Yuliani Hana Eliyani Hani Plumeriastuti Herry Agoes Hermadi Hidayatik, Nanik Indah Norma Triana, Indah Norma Indira Vania Carisya Ira Sari Yudaniyanti Ismudiono Ismudiono, Ismudiono Joel Jeevan Raj S/O Yogarajah Johansyah, Fadilla Anita Kinksky Kadek Rachmawati Kadek Rachmawati Kuncoro Puguh Santoso Kuncoro Puguh Santoso Legowo, Djoko Lilik Maslachah Lita Rakhma Yustinasari Magdalena Eki Pertiwi Mas’ud Hariadi Mirni Lamid Mochamad Lazuardi Muhammad Thohawi Elziyad Purnama Nidia Yulanda Nidom, Chairul Anwar Nufus, Faizah Zakiyyatun Nur Emma Suriani Nusdianto Triakoso Poetranto, Emmanuel Djoko Praja, Ratih Novita Pratama Putra Banusiandana Pudji Srianto Rahmawati, Kadek Rahmi Sugihartuti Ratna Damayanti Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Rochmah Kurnijasanti ROHMAH, LAILATUL Santoso, Kuncoro Puguh Saputro, Amung Logam Setiawan Koesdarto Shabrina Fauzia Prayoga Sherina Lashita Candrakirana Soeharsono Sri Hidanah Sri Mulyati Sri Pantja Madyawati Srimaryanto, Leonardo Reza Suherni Susilowati Sukmanadi, Moh. Sukmanadi, Mohammad Sulaiman, Azhari Athaillah Sunarso, Agus Suzanita Utama Syahraini Adhiya Lathifah Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Umi Farichah Bascha Wahyuni, Retno Sri Warsito, Sunaryo Hadi Widiyatno, Thomas Valentinus Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiranata, Try Ardhi Wisudawati, Feby Dwi Wiwik Misaco Yuniarti Wurlina, W Yunita, Maya Nurwartanti