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All Journal Majalah Kedokteran Bandung Medula Juke Indonesian Journal of Cancer Turbo : Jurnal Program Studi Teknik Mesin Jurnal Aplikasi Teknologi Pangan Jurnal Pengabdian Pada Masyarakat Jurnal Aplikasi Teknologi Pangan Jurnal Ilmu Kedokteran dan Kesehatan Bioscientia Medicina : Journal of Biomedicine and Translational Research Jurnal Kesehatan Ilmiah Indonesia / Indonesian Health Scientific Journal Review of Primary Care Practice and Education (Kajian Praktik dan Pendidikan Layanan Primer) Jurnal Mandala Pengabdian Masyarakat Jurnal Kesehatan Tambusai MAHESA : Malahayati Health Student Journal Bioscientia Medicina : Journal of Biomedicine and Translational Research JURNAL AGROINDUSTRI HALAL International Journal of Health and Pharmaceutical (IJHP) JUSTICIA SAINS: Jurnal Ilmu Hukum Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Sains Medisina JPM (Jurnal Pengabdian Masyarakat) Ruwa Jurai BEGAWI: Jurnal Pengabdian Kepada Masyarakat Jurnal Agromedicine Unila: Jurnal Kesehatan dan Agromedicine
Asep Sukohar
Department Of Pharmacology; Faculty Of Medicine; Universitas Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Indonesian Journal of Cancer

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Peran Asam Klorogenat Terhadap Ekspresi Gen; miRNA 146 A, Caspase 3, Cyclin D1, dan Kematian Sel Kanker Hepatoseluler Model Cell Lines Hep-G2 SUKOHAR, ASEP; HERAWATTI, HENING; WITARTO, ARIEF B
Indonesian Journal of Cancer Vol 8, No 1 (2014): Jan - Mar 2014
Publisher : "Dharmais" Cancer Center Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (636.82 KB)

Abstract

Chlorogenic acid (CA) is the active compound isolated from medicinal plants, can be used as a chemopreventive agents of hepatocellular cancer (HCC). It works as an exogenous antioxidant and inhibit growth of cancer cells through the inhibition of free radicals. The purpose of this study is to know mechanism of CA in inhibition to growth of Hep-G2 through apoptosis stimulation. In vitro study was performed Hep-G2 cells. The samples were divided into the control group treatment and experiment group expose to 727, 500 and 250 ?M and 3 times repetition. Expression of miRNA 146 A, caspase 3 and cyclin D1 examined by RT-PCR CFX-96. Samples were analysed at 0, 8, 18, and 24 hours after exposure CA. The data were statistically tested by repeated measurement, pearson, and multivariate regression. The results showed that cell death of Hep-G2 were increase as the dose increase and time, at 8 hours after exposure CA cell death of Hep-G2 increased as much as 35,68, 37,75, 40,86%. At 18 hours cell death of Hep-G2 increased as much as 54,56, 56,48, and 59,73%. At 24 hours cell death of Hep-G2 increased as much as 67,73, 69,37, and 72,16%. The lowest expression miRNA 146 A in group 24 hours after exposure at doses of 727 ?M CA (0,85), followed by 500 ?M (1,28) and the highest expression at a dose of 250 ?M (1,61). The result of repeated measurement test: miRNA 146 A and caspase 3 at 8th and 18th hours was significantly different to the 24th with p<0.05. The expression of caspase 3 increase from 0-24 hours, the highest expression of caspase 3 in group 18 hours after exposure at doses CA at 750 ?M (3,86). After 18 hours, expression caspase 3 decreased and the lowest in group 24 hours after exposure: at a dose of 250 ?M (1,52). Expression of cyclin D1 decrease from 0-24 hours with the highest expression at 0 hours (4,35) at a dose of 250 ?M and the lowest expression at 24 hours after exposure to CA (0,32) at a dose of 727 AK ?M.
Peran Asam Klorogenat Terhadap Ekspresi Gen; miRNA 146 A, Caspase 3, Cyclin D1, dan Kematian Sel Kanker Hepatoseluler Model Cell Lines Hep-G2 SUKOHAR, ASEP; HERAWATTI, HENING; WITARTO, ARIEF B
Indonesian Journal of Cancer Vol 8, No 1 (2014): Jan - Mar 2014
Publisher : Indonesian Journal of Cancer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (636.82 KB)

Abstract

Chlorogenic acid (CA) is the active compound isolated from medicinal plants, can be used as a chemopreventive agents of hepatocellular cancer (HCC). It works as an exogenous antioxidant and inhibit growth of cancer cells through the inhibition of free radicals. The purpose of this study is to know mechanism of CA in inhibition to growth of Hep-G2 through apoptosis stimulation. In vitro study was performed Hep-G2 cells. The samples were divided into the control group treatment and experiment group expose to 727, 500 and 250 ?M and 3 times repetition. Expression of miRNA 146 A, caspase 3 and cyclin D1 examined by RT-PCR CFX-96. Samples were analysed at 0, 8, 18, and 24 hours after exposure CA. The data were statistically tested by repeated measurement, pearson, and multivariate regression. The results showed that cell death of Hep-G2 were increase as the dose increase and time, at 8 hours after exposure CA cell death of Hep-G2 increased as much as 35,68, 37,75, 40,86%. At 18 hours cell death of Hep-G2 increased as much as 54,56, 56,48, and 59,73%. At 24 hours cell death of Hep-G2 increased as much as 67,73, 69,37, and 72,16%. The lowest expression miRNA 146 A in group 24 hours after exposure at doses of 727 ?M CA (0,85), followed by 500 ?M (1,28) and the highest expression at a dose of 250 ?M (1,61). The result of repeated measurement test: miRNA 146 A and caspase 3 at 8th and 18th hours was significantly different to the 24th with p<0.05. The expression of caspase 3 increase from 0-24 hours, the highest expression of caspase 3 in group 18 hours after exposure at doses CA at 750 ?M (3,86). After 18 hours, expression caspase 3 decreased and the lowest in group 24 hours after exposure: at a dose of 250 ?M (1,52). Expression of cyclin D1 decrease from 0-24 hours with the highest expression at 0 hours (4,35) at a dose of 250 ?M and the lowest expression at 24 hours after exposure to CA (0,32) at a dose of 727 AK ?M.
Co-Authors A.Yudi Eka Risano Abighail, Nistita Aditya Ramadhon Islami Sakaganta Afriyana, Regi Afriyani Afriyani Aghesna Rahmatika Kesuma Ahmad Fitri, Ahmad Akbar, Muhammad Zakky Putra Akmal Ghani, Muhammad Ali, Nur Fitriana Muhammad Alifah, Ghina Nur Ambarwati, Endah Aminuyati Andi Nafisah Andi Nafisah, Andi Anisa Nuraisa Jausal Anita Febrina, Anita Aqsha, Shabita Arianti, Tanti Arief B Witarto Ariyati, Retno Desy Arli Suryawinata Arli Suryawinata Arsyad Sobby Kesuma Aulia Ramdini, Dwi Aulian Mediansyah Baddarudin, Mohammad Bainah Sari Dewi Betta Kurniawan, Betta Christine Wulandari Damayanti, Ervina Daulay, Suryani Agustina Dharmesti, Rizky Aleyda Dyah Indriana Kusumastuti Elisa, Syavira Emily Rachman, Reimma Endah Ambarwati Endah Setyaningrum, Endah Endro Prasetyo Wahono Eva Lusina, Septia Fala, Arzety Rifda Farhan Akbar, Muhamad Febriyanti, Triana Fibra Nurainy Fitria Saftarina Fredison, Fredison Gerliandi, Egi Oktarian Hanna Mutiara, Hanna Hardito, Joni Hatimi, Fahmi Ilham Hendra Tarigan Sibero HENING HERAWATTI, HENING Herri S. Sastramihardja Ida Nurhaida Ihsanti Dwi Rahayu Indri Windarti Intan Kusumaningtyas Irza Sukmana, Irza Jausal, Anisa Nuraisa Josi Jeremia Manurung Junando, Mirza Karima, Nisa Kaskoyo, Hari Kurniawaty, Evi Kylalona, Gracylia L.P, Eka Ananda LP, Eka Ananda Maherariyat, Maherariyat Mallarangeng, Andi Nafisah Tendri Adjeng Mediansyah, Aulian Morfi, Chicy Widya Muhammad Fitra Wardhana Sayoeti Muhammad Iqbal Muhammad Iqbal Ramadhan Muhartono Muhartono Muhartono Muhartono Muhartono Mukhlis Imanto Mutiara, Sephia Aruma Nisa, Sayyidatun Noverman Duadji Noviani, Erina Novita Carolia Novita Herdiana Novita Tresiana NUR AFRIYANI Nurmasuri, Nurmasuri Oktafany, Oktafany Oktoba, Zulpakor Pardilawati, Citra Yuliyanda Permana, Km. Allan Wahyu Permana, M. Agung Yudistira Puteri, Vadiyani Fricillya Putri Idrus, Indrayati Putri, Asyifa Adinda Putri, Meilani Putri, Meilani Dwi Putri, Oka Mahila Gustia Ramadhan Triyandi Ramadhina, Farrasyifa Ramdini, Dwi Aulia Rani Himayani Rasmi Zakiah Oktarlina Ratna Dewi Puspita Sari Ratna, Maya Ganda Rika Lisiswanti Rizqa Atina Safitri, Nara Said, Feriany Salam, Deby Mipa Samsu Udayana Nurdin Samsu Udayana Nurdin Samsul Bakri Samsul Rizal Santika, Mesi Sepriyadi Adhan S Setiawan, Gigih Shinta Mayasari Siboro, Erwi Saulina Venezia Slamet Budi Yuwono Sofyan Musabiq Wijaya Sofyan Musyabiq Wijaya Subeki Sugiyanto - Suharmanto Suharyani Suharyani Suharyani Sumekar, Diyah Suri, Nurma Suryadi Islami Suryawinata, Arli Sutarto Sutarto Sutarto Sutarto Syazili Mustofa Thahir, Salsabila Anggraini Trijayanthi, Winda Triyandi, Ramadhan Wahdah, Annisa Shohifatul Wardani, Farah Dwi Wardhana S, Muhammad Fitra Wardhana, M. Fitra Wijaya, Sofyan Wijaya, Sofyan Musabiq Winda Trijayanthi Utama, Winda Trijayanthi WS, Muhammad Fitra Yofita Sulfiana Sundari Zeta, Nathasya Karren