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Development of Siganid (Siganus guttatus) larvae during the transition period Darsiani Darsiani; Mia Setiawati; Dedi Jusadi; Muhammad Agus Suprayudi; Asda Laining
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22296

Abstract

Siganid is better known as rabbit fish. In hatcheries, constraint that is still faced is the low survival, which was assumed to occur because of the timing for initial feeding is not solidly known. This research aimed to examine the best initial feeding time for siganus, based on evaluation on eyes and yolk reserves during the transition. The research was conducted from 24-29 March 2021 in IPUW Barru, South Sulawesi. Larvae were obtained from the second progeny (G2) of domesticated Siganus guttatus. Larvae were reared for 5-6 days without feeding. Evaluated parameters include eyes diameter and yolk reserves. Samples were observed with microscope and will be explained descriptively. Water quality parameters were measured, namely DO, salinity, pH and temperature. Eyes diameter at 6 Hour After Hatching (HAH) ranges between 81.5-128.9 m, 13 HAH= 125.5-167.7 m, 24 HAH= 138.2-213.9 m, two days after hatching 2 Day After Hatching (DAH) = 113.6-193.1 m, 3 DAH= 163.1-219.2 m, 4 DAH= 190.4-212.6 m. Yolk reserves diameter ranged between 137-194m (6 HAH), 13 HAH= 152-191m, 24 HAH= 94.0-185m, 2 DAH= 75.3-99.63m, 3 DAH= 42.33-87.58m, 4 DAH= 38.17-55.59m. After age 5 DAH, there are no larvae found alive (dead). Eyes developed at age 6 HAH and experienced pigmentation at age 24 HAH. Conversely, yolk reserves diameters were getting smaller since age 24 HAH and completely disappear at age 4 DAH. It indicates that eyes effectively see feeds at age 2 DAH. Therefore, initial feeding should be started. The water quality parameters measured were still in normal conditions according to the life of S. guttatus larvae. From this research, it can be concluded that eyes have been well functioned at age 2 DAH and yolk reserves was finished at age 4 DAH. Therefore, the initial feeding should be done at the age of 2 DAH.Keywords:Development, Siganus guttatus larvae,Transition
Effect of feedings with different protein levels and dietary supplemental rElGH on culture performances of sex reversed Oreochromis niloticus (Linnaeus, 1758) Muhammad Safir; alimuddin alimuddin; Mia Setiawati; muhammad Agus Suprayudi; Muhammad Zairin Junior
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22550

Abstract

This study aims to evaluate the culture performance of tilapia (Oreochromis niloticus) that have been treated with 17-methyltestosterone (MT) and without MT (w-MT), feed with different protein levels (20, 24, and 28%) and recombinant Epinephelus lanceolatus growth hormone (rElGH)-diet. The research was conducted in 9 treatments and triplicate. Tilapia larvae were soaked twice, totaling 500 larvae, at the age of 10 days after hatching (DAH) and the age of 14 DAH was soaked for 4 hours using 1/l MT 2 mg/l solution. Fish maintenance was conducted in an aquarium of 1.0x0.5x0.5 m3 in the first month, and three months later in net cages (2.0x2.0x1.5 m3). Daily growth rate (DGR) and biomass gain (BG) were increased in line with increasing feed protein content and rElGH supplementation. The highest DGR and BG values were MT+28+rElGH (P 0.05) treatment. The highest feed consumption and the lowest feed conversion ratio were also obtained in the MT+28+rElGH treatment (P 0.05). Fish survival was ranged from 79.89 to 90.28% (P 0.05). The highest profit potential was found in the MT+28+rElGH treatment. The efficient aquaculture can be obtained by feeding sex-reversed tilapia at a protein level of 28% and a diet supplemented with rElGH.Keywords:Feed conversion ratioGrowth hormoneProtein retention17-methyltestosterone
INFLUENCE OF TAURINE ON LARVAL EYE DEVELOPMENT AND GROWTH PERFORMANCE OF GOLDEN RABBITFISH (Siganus guttatus) Darsiani Darsiani; Mia Setiawati; Dedi Jusadi; Muhammad Agus Suprayudi; Asda Laining
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.2057

Abstract

ARTICLE HIGLIGHTS- Taurine improves eye development in golden rabbitfish larvae- Enhanced growth performance with taurine-enriched rotifers- Taurine positively impacts fish eye and body growthABSTRACTGolden rabbitfish (Siganus guttatus) is an emerging species for aquaculture industry, despite constrains faced on commercial scale of seed production due to the high mortality during the first-feeding stage. An experiment was conducted to determine the effect of taurine through enrichment of rotifers as live-feed on eye development and growth performance of larval golden rabbitfish. Observation of eye development was carried out by measuring eye diameter of the larva and development of the retina based on histology assessment. Growth performance was measured for absolute growth and fin development of the larvae and survival rate. The results showed that taurine-enriched rotifer generally provided better performances for eye development and growth of larval golden rabbitfish compared with control (without taurine). Increase of taurine dose up to 0.050 g/L resulted in an increase of eye diameter, absolute growth, fin development and survival rate. Further increased increment of the taurine tended to decrease the values of the measured parameters. The eye diameter of larval golden rabbitfish fed with 0.050 g/L taurine-enriched rotifer was significantly wider (106.1±9.8 µm) (P < 0.05) compared with control (58.2±14.3 µm), but did not significantly differ from other doses of taurine (P > 0.05). Body width of larvae fed with 0.050 g/L taurine-enriched rotifer was significantly higher (127.3±14.6 µm) (P < 0.05) compared with control (98.8±18.3 µm). In regard to eye development, growth performances (total length, fin development and survival rate), dose of taurine for rotifer enrichment fed to larval golden rabbitfish Siganus guttatus was 0.050 g/L.
Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy) Dian Novita Sari; Hasan Nasrullah; Julie Ekasari; Muhammad Agus Suprayudi; Alimuddin Alimuddin
Biodiversitas Journal of Biological Diversity Vol. 22 No. 2 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220227

Abstract

Abstract. Sari DN, Nasrullah H, Ekasari J, Suprayudi MA, Alimuddin A. 2021. Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy). Biodiversitas 22: 741-750. Glucokinase (GCK) is one of the enzymes that play important roles in carbohydrate metabolism and high glucose homeostatic in fish. The information about the GCK mRNA sequence and its expression is limited in Giant gourami, one of the most important herbivorous aquaculture species in Indonesia. The present study aimed to characterize the GCK mRNA and analyze its mRNA expression and plasma glucose levels after high glucose injection in Giant gourami. We also compared its sequence variability among carnivorous and herbivorous fish. The GCK mRNA was identified using polymerase chain reaction (PCR) method from the fish liver. Its mRNA level was analyzed by real-time PCR (qPCR). Giant gourami GCK mRNA sequence was 2104 nucleotide long, encoding 478 amino acids, and shared high similarity with other fish. GCK was mainly expressed in the liver. The mRNA level of GCK was highly up-regulated after 6 hours of high glucose injection, in-line with the plasma glucose in the blood. There are no major differences observed in the GCK amino acid sequences among Giant gourami and other fish. The knowledge gained from this study could be used as a reference for further exploration of metabolic regulation in Giant gourami.
The Growth Performance of Tilapia (Oreochromis sp.) using Different Doses of Organic Cooper (Cu–O) in the Diets Dedi Jusadi; Yessy Rahmayanti; Muhammad Raditya Gumelar; Talita Shofa Adestia; Muhammad Agus Suprayudi
Journal of Aquaculture and Fish Health Vol. 15 No. 1 (2026): JAFH Vol. 15 No. 1 February 2026
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v15i1.78070

Abstract

Copper (Cu-O) is a micromineral required in small amounts in the body, yet it plays an important role in the metabolism and health of fish. This study aimed to evaluate the growth performance of tilapia (Oreochromis sp.) fed diets supplemented with organic copper (Cu-O). Four treatments were tested: K (control, without Cu-O), Cu-O 1 (1 mg kg⁻¹ Cu-O), Cu-O 2 (2 mg kg⁻¹ Cu-O), and Cu-O 3 (3 mg kg⁻¹ Cu-O). Fifteen tilapia with an average initial weight of 8.75 ± 0.02 g were reared in aquaria (60 × 50 × 40 cm, water depth 30 cm) for 60 days. Fish were fed to satiation three times daily. The results showed that the growth parameters of tilapia fed Cu-O-supplemented diets were significantly higher (P < 0.05) than those of the control. The optimal dose of Cu-O supplementation was found to be 2 mg kg⁻¹ feed; subsequent studies should investigate interspecies variations and refine dosage recommendations accordingly.
Co-Authors , Alimuddin , Sarmin . Ridwan . Vera Dewi Mulia, Troef Soemarno A Halim A. Malik Serang A. Shofy Mubarak Achmad Noerkhaerin Putra Adang Saputra Adiasmara Giri, I Nyoman Agus Oman Sudrajat Ahmad Yazid Latif Aimma, Muhammad Ariful Akhmad Baihaqi Alimuddin alimuddin alimuddin Amelia Oktaviani, Amelia Anargya Pradipa Tristanandana Apriana Vinasyiam Aprilianti Pratiwi Arief Boediono Aris Sudiyanto Aris Tri Wahyudi Arradini, Dewi Artin Indrayati Asda Laining Asda Laining Atmojo, Joko Tri Azmi Afriansyah Benny Heltonika Burhanudin Faisal, Burhanudin D. Jusadi Dairun, Suclyadi DARMAWAN SETIA BUDI Darsiani Darsiani Darsiani Darsiani Deddy Yaniharto Deddy Yaniharto Dedi Jusadi Dedi Jusadi DEDI JUSADI Dedi Yaniharto dedy yaniharto Denny Wahyudi DIAH AYU SATYARI UTAMI Diamahesa, Wastu Ayu Dian Novita Sari Didi Humaedi Yusuf, Didi Humaedi Dinamella Wahjuningrum Dinar Tri Soelistyowati Dini Harianto Dodi Hermawan E. Mursitorini Eddy Supriyono Edy Marsudi Elas, Putri Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja Endriatmo Soetarto F. Hapsyari Faiqotul Himmah, Maihardiyanti Fatma Hajiali Fauzan Fredinan Yulianda Gadis Sri Haryani Gebbie Edriani Ghina Raudathul Jannah Giri Maruto Darmawangsa, Giri Maruto Hadi Pratama, Ricky Harton Arfah Hasan Abidin Hasan Nasrullah Hasan Nasrullah Hasrah Hasrah Helena Sahusilawane Hendriana, Andri Husnidar Husnidar I Dewa Putu Oka Suardi I Gede Setiawan Adi Putra I Gusti Agung Gede Wiadnyana I GUSTI AGUNG GEDE WIADNYANA . I Gusti Ayu Rai I MADE ARTIKA I Made Artika I Wayan Budiyasa I Wayan Nurjaya I Wayan Suanda I. Mokoginta Ichsan Achmad Fauzi Iis Diatin Imah, Nenden Imlani, Ainulyakin Hasan Inarto, Hendri ING MOKOGINTA Iqbal Kurniawinata, Mohamad Ira Akhdiana Irmawati Irvan Faizal Irzal Effendi Ismarica, Ismarica Jr., Muhammad Zairin Juli Ekasari Julie Ekasari Jullie Ekasari KATSUYUKI HAMASAKI KATSUYUKI HAMASAKI KETUT BUDI SUSRUSA Komang Adi Wika Wiguna Kusriyati Kusriyati L. Indriastuti Latifah Kosim Darusman Ligaya I. T. A. Tumbelaka M. Ikhsan M. Setiawati M. Tri Djoko Sunarno M. Zairin Junior Mas Tri Djoko Sunarno Meilisza, Nina Meritha, Wellya Wichi Mia Setiawati Muhaimin Hamzah Muhammad Jailani Muhammad Junior Zairin Muhammad Raditya Gumelar Muhammad Ridzky Adhory Muhammad Roikhan Amanullah Muhammad Safir Muhammad Zairin Jr Muhammad Zairin JR Muhammad Zairin Jr Muhammad Zairin Jr Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. MUHAMMAD ZAIRIN Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mulyasari Mulyasari Munawar Khalil Muslim Muslim Muzahar Niagara, Niagara Nina Meilisza Nur Bambang Priyo Utomo Nuraini Annisa, Nuraini Nuril Farizah Odang Carman Otie Dylan Soebhakti Hasan Padlilah, Rahmi Paramadina, Siwi Pattipeilohy, Christian Ernsz R Rifai R. Sutajaya Raditya Gumelar, Muhammad Rahminiwati, Min Rakhmawati, Rakhmawati, Ria Septy Anggraini, Ria Septy Ricky Ramadhan Ridhia Putri RIDWAN AFFANDI Rina Hirnawati Rizky Nafaida Samalei, Ermianus Sarwititi Sarwoprasojdo, Sarwititi Senja, Reza Karunia Setyorini, Catur Siti Komariyah Sri Nuryati Sri Nuryati Suardi Laheng Suci Antoro Suci antoro Suclyadi Dairun Sukenda, Sukenda, Supriyadi Hari Respati Suryadi Saputra T. Makmur Talita Shofa Adestia Tatag Budiardi Titin Kurniasih TOSHIO TAKEUCHI TOSHIO TAKEUCHI Tulas Aprilia Upmal Deswira Utomo, Nurbambang Priyo Wahyudi, Imam Tri Wasmen Manalu Wida Lesmanawati WIDANARNI WIDANARNI Widiyanto, Aris Wijianti, Hani Wijiwinarsih, Amallia Wika Wiguna, Komang Adi Winda Styani Irawan, Winda Styani Wiyoto Wiyoto Y. Hadiroseyani Yessy Rahmayanti Yogo Tri Saloko Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yutaka Haga Zairin Jr., Muhammad Zinatul Hayati