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Development of Siganid (Siganus guttatus) larvae during the transition period Darsiani Darsiani; Mia Setiawati; Dedi Jusadi; Muhammad Agus Suprayudi; Asda Laining
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22296

Abstract

Siganid is better known as rabbit fish. In hatcheries, constraint that is still faced is the low survival, which was assumed to occur because of the timing for initial feeding is not solidly known. This research aimed to examine the best initial feeding time for siganus, based on evaluation on eyes and yolk reserves during the transition. The research was conducted from 24-29 March 2021 in IPUW Barru, South Sulawesi. Larvae were obtained from the second progeny (G2) of domesticated Siganus guttatus. Larvae were reared for 5-6 days without feeding. Evaluated parameters include eyes diameter and yolk reserves. Samples were observed with microscope and will be explained descriptively. Water quality parameters were measured, namely DO, salinity, pH and temperature. Eyes diameter at 6 Hour After Hatching (HAH) ranges between 81.5-128.9 m, 13 HAH= 125.5-167.7 m, 24 HAH= 138.2-213.9 m, two days after hatching 2 Day After Hatching (DAH) = 113.6-193.1 m, 3 DAH= 163.1-219.2 m, 4 DAH= 190.4-212.6 m. Yolk reserves diameter ranged between 137-194m (6 HAH), 13 HAH= 152-191m, 24 HAH= 94.0-185m, 2 DAH= 75.3-99.63m, 3 DAH= 42.33-87.58m, 4 DAH= 38.17-55.59m. After age 5 DAH, there are no larvae found alive (dead). Eyes developed at age 6 HAH and experienced pigmentation at age 24 HAH. Conversely, yolk reserves diameters were getting smaller since age 24 HAH and completely disappear at age 4 DAH. It indicates that eyes effectively see feeds at age 2 DAH. Therefore, initial feeding should be started. The water quality parameters measured were still in normal conditions according to the life of S. guttatus larvae. From this research, it can be concluded that eyes have been well functioned at age 2 DAH and yolk reserves was finished at age 4 DAH. Therefore, the initial feeding should be done at the age of 2 DAH.Keywords:Development, Siganus guttatus larvae,Transition
Effect of feedings with different protein levels and dietary supplemental rElGH on culture performances of sex reversed Oreochromis niloticus (Linnaeus, 1758) Muhammad Safir; alimuddin alimuddin; Mia Setiawati; muhammad Agus Suprayudi; Muhammad Zairin Junior
Depik Vol 11, No 1 (2022): April 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.1.22550

Abstract

This study aims to evaluate the culture performance of tilapia (Oreochromis niloticus) that have been treated with 17-methyltestosterone (MT) and without MT (w-MT), feed with different protein levels (20, 24, and 28%) and recombinant Epinephelus lanceolatus growth hormone (rElGH)-diet. The research was conducted in 9 treatments and triplicate. Tilapia larvae were soaked twice, totaling 500 larvae, at the age of 10 days after hatching (DAH) and the age of 14 DAH was soaked for 4 hours using 1/l MT 2 mg/l solution. Fish maintenance was conducted in an aquarium of 1.0x0.5x0.5 m3 in the first month, and three months later in net cages (2.0x2.0x1.5 m3). Daily growth rate (DGR) and biomass gain (BG) were increased in line with increasing feed protein content and rElGH supplementation. The highest DGR and BG values were MT+28+rElGH (P 0.05) treatment. The highest feed consumption and the lowest feed conversion ratio were also obtained in the MT+28+rElGH treatment (P 0.05). Fish survival was ranged from 79.89 to 90.28% (P 0.05). The highest profit potential was found in the MT+28+rElGH treatment. The efficient aquaculture can be obtained by feeding sex-reversed tilapia at a protein level of 28% and a diet supplemented with rElGH.Keywords:Feed conversion ratioGrowth hormoneProtein retention17-methyltestosterone
Immersion Effect of Estradiol-17β on Cannibalism of Asian Redtail Catfish (Hemibagrus nemurus) Post Larvae Benny Heltonika; Agus Oman Sudrajat; Muhammad Junior Zairin; Widanarni Widanarni; Muhammad Agus Suprayudi; Wasmen Manalu; Yani Hadiroseyani
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.38173

Abstract

Highlight Research The dose, duration of immersion, and time interval of immersion of the estradiol-17β affect the rate of cannibalism and increase the seed survival of Asian redtail catfish in low treatment in this research. Over optimum treatment increased the incidence of cannibalism at Asian redtail catfish and decrease growth performance. Treatment of estradiol-17β, give the ability of Asian redtail catfish to live on high density The treatment of estradiol-17β, has an effect on concentration plasma of body testosterone and cortisol   Abstract The cannibalistic behavior of Asian redtail catfish greatly hampers the supply of these fish seeds, invoking the need to be controlled. Estradiol is one of the hormones that has been known to reduce cannibalistic behavior on fish. This study aimed to evaluate the effect of estradiol-17β immersion on the incidence of cannibalism in the rearing of post larvae of Asian redtail catfish. Post larvae was treated with a combination of doses of estradiol hormone 0, 1, and 2 ppm and immersion time of two and four hours with immersion intervals of three and six days with a completely randomized design. Each treatment had a fish density of 10 fish L-1, with mean size individual length of 6.73 ± 0.73 mm (three days of age after hatched), with four replications. Fish were fed with tubifex up until satiation point and reared for 30 days. The results showed that the administration of the estradiol-17β through immersion with a dose of 1 ppm for two hours and interval of six days was able to reduce the level of cannibalism and increase the survivability. A further increase in dose, immersion time, and interval had the opposite effect. Estradiol-17β immersion with certain dose, duration, and interval affected the rate of cannibalism, growth rate, and survival. Estradiol-17β immersion at a dose of 1 ppm for two hours and six days interval was the best to reduce the level of cannibalism and normal mortality in post larvae of Asian redtail catfish.
CANNIBALISM PERFORMANCE OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) FED RATION SUPPLEMENTED WITH DIFFERENT DOSES OF 17α-METHYLTESTOSTERONE Heltonika, Benny; Sudrajat, Agus Oman; Junior, Muhammad Zairin; Widanarni, Widanarni; Suprayudi, Muhammad Agus; Manalu, Wasmen; Hadiroseyani, Yani
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.27-35

Abstract

One of the obstacles in Asian redtail catfish hatchery is the high cannibalism incidence. Cannibalism is associated with aggressive behavior caused by hormonal metabolism especially of testosterone. The purpose of this study was to evaluate the effect of testosterone administration on the cannibalism incidence in the Asian redtail catfish juveniles.  The experiment was conducted by rearing fish with initial length of 4.09±0.19 cm in 16 of 20 L aquariums with a density of 2 fish L-1. The experiment was designed with a completely randomized design with 4 treatments which were different level of 17α-methyltestosterone supplementation in feed, i.e., 0 mg kg-1 feed (A) as control, 7.5 mg kg-1 feed (B), 15 mg kg-1 feed (C), and 30 mg kg-1 feed (D). Each treatment has 4 replications. Fish fed experimental diet (40% protein) 4 times a day to satiation for 30 days. The parameters observed were type and index of cannibalism, aggressive behavior, survival rate, normal mortality, growth performance, hormones concentrations (estradiol, testosterone, and cortisol), and water quality. The results showed that cannibalism type II (the fish eaten completely or missing) and cannibalism index increased with the increasing doses of testosterone administration in the feed with the highest cannibalism incidence was 40.63%. The highest survival rate was found in treatment B (73.75±2.50%) and was not significantly different from the control treatment (69.38±2.39%). No differences in testosterone concentration and in the growth performance among the treatments.  However, there was a trend of decrease in the estradiol concentration of Asian redtail catfish juveniles fed ration supplemented with the increasing doses of 17α-methyltestosterone. Based on the results obtained in this research, estradiol changed in the body's plasma, it appears that there was a role for plasma estradiol concentration in controlling cannibalism of Asian redtail catfish juveniles. It concluded that the testosterone administration affected the cannibalism incidence in the Asian redtail Catfish. 
Effectiveness of NSP enzyme and organic chromium supplementation in low-protein feed on carbohydrate utilization in Pomfret Colossoma macropomum Faiqotul Himmah, Maihardiyanti; Achmad Fauzi, Ichsan; Ekasari, Julie; Raditya Gumelar, Muhammad; Muhammad Agus Suprayudi
Jurnal Akuakultur Indonesia Vol. 24 No. 1 (2025): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.24.1.21-31

Abstract

This study aims to evaluate the effectiveness of NSP enzyme and organic chromium supplementation in low-protein Colosoma macropomum diet to increase carbohydrate utilization. The diets contains isoprotein (20%) and isoenergy. Factorial design with two factors, namely NSP enzyme (0 and 1 g/kg) and organic chromium (0, 1, and 2 mg/kg). Fish are reared in an aquarium measuring 80 x 50 x 35 cm3 and containing 75 L of water and stock at the density of 15 fish and reared for 60 days. The test parameters used are postprandial blood glucose levels, antioxidant activity, glycogen in muscle and liver, and growth performance. The results showed that the highest postprandial blood glucose levels were when the NSP enzyme was administered. Furthermore, the lowest malondialdehyde (MDA) values and the highest superoxide dismutase (SOD) and glutathione peroxide (GPx) values were obtained in fish fed feed containing 1 g/kg of NSP enzyme and 1 mg/kg organic chromium. Fish fed feed containing 1 g/kg NSP enzyme and 1 mg/kg organic chromium showed significant values in liver and muscle glycogen as well as growth performance compared to other treatments. From this research, it can be concluded that supplementation of NSP enzyme and organic chromium at doses of 1 g and 1 mg per kg of feed produces the best growth performance and anti-oxidant capacity in pomfret fish. Keywords: antioxidant, glycogen, growth performance, postprandial blood glucose ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efektivitas suplementasi enzim NSP dan kromium organik pada pakan ikan bawal Colosoma macropomum rendah protein untuk meningkatkan pemanfaatan karbohidrat. Pakan yang digunakan mengandung iso protein (20%) dan isoenergi. Rancangan faktorial dengan dua faktor yakni enzim NSP (0 dan 1 g/kg) dan kromium organik (0, 1, dan 2 mg/kg). Ikan bawal dipelihara dalam akuarium dengan ukuran 80 x 50 x 35 cm3 dan berisi air 75 L diisi 15 ekor ikan dan dipelihara selama 60 hari. Adapun parameter uji yang digunakan adalah pengukuran kadar glukosa darah postprandial, aktivitas antioksidan, glikogen, dan kinerja pertumbuhan. Hasil penelitian menunjukkan kadar glukosa darah postprandial tertinggi pada pemberian enzim NSP. Selanjutnya nilai malondialdehyde (MDA) terendah dan superoxide dismutase (SOD) dan glutathione peroxide (GPx) tertinggi didapat pada ikan yang diberi pakan mengandung pada 1 g/kg enzim NSP dan 1 mg/kg kromium organik. Ikan yang diberi pakan yang mengandung 1 g/kg enzim NSP dan 1 mg/kg kromium organik menunjukkan nilai signifikan pada glikogen hati dan otot serta kinerja pertumbuhan dibanding perlakuan lainnya. Dari penelitian ini dapat disimpulakan bahwa suplementasi enzyme NSP dan Kromium organik pada dosis 1 g dan 1 mg per kg pakan menghasilkan kinerja pertumbuhan dan kapasitas antioksidan terbaik pada ikan bawal. Kata kunci: antioksidan, glikogen, glukosa darah postprandial dan kinerja pertumbuhan
Effect of Kind and Dosage of Enrichment Materials on the Nutritional Quality of Rotifers Especially n3-HUFA Suprayudi, Muhammad Agus
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.659 KB) | DOI: 10.19027/jai.2.21-25

Abstract

This experiment was conducted to evaluate the quality of rotifer enriched with four  kinds of enrichement materials from the stand point of essensial fatty acids. Rotifer was enriched at 24 - 25oC for 18 hours at a density of 1000 ind/ml.  Rotifers were treated by four kinds of enrichment materials such as oleic acid (R-OA), two different density of Nannochloropsis oculata, (4x107 and 16x107 cell/ml; R-N18 and R-N42) two different levels of eicosapentaenoic acids (EPA) triglyceride type (EPA-TAG) (20 and 40 ml/ml; R-E20, R-E40) and two different level of EPA ethyl ester (EPA-EE) (R-EE25 and R-EE50%) respectively. Rotifers enriched with Nannochloropsis oculata and EPA-EE type have a similar profile of essensial fatty acid especially on n3-HUFA that dominated by EPA, while DHA was in a trace amount or not detected.  In addition Nannochloropsis oculata as an enrichment material showed the highest population density of rotifers during enrichment periods.  Rotifer enriched with EPA-TAG has a more complete of essential fatty acid profile compared to other enrichment materials due to their contained both of EPA and DHA. We conclude that rotifer enriched with EPA-TAG as enrichment material showed the best nutritional quality of rotifers from the stand point of essential fatty acid. Key words :  Rotifers, enrichment, eicosapentaenoic acid, docosaheksaenoic acid,  n3-HUFA   ABSTRAK Penelitian ini dilakukan untuk mengetahui profil asam lemak rotifera yang diperkaya dengan berbagai macam jenis dan dosis pengkaya. Rotifera dengan kepadatan 1000 ind./ml diperkaya dengan bebagai bahan pengkaya seperti asam oleat (R-OA); Nannochloropsis oculata dengan kepadatan 4x107 dan 16x107 sel/ml (R-N18 dan R-N42); eicosapentaenoic acid (EPA) tipe triglicerida (EPA-TAG)  dengan dosis 20 dan 40 μl (R-E20 dan R-E40) dan EPA tipe ethyl esther (EPA-EE)  dengan dosis 25 dan 50 μl per liter (R-EE25 and R-EE50%). Rotifera diperkaya selama 18 jam pada suhu 24-25oC. Rotifera yang diperkaya dengan Nannochloropsis oculata serta EPA-EE memiliki kesamaan profil asam lemak terutama pada incorporasi EPA, sedangkan DHA terkandung pada jumlah yang kecil atau tidak terdeteksi.  Adapun rotifera yang diperkaya dengan EPA-TAG memiliki profil asam lemak yang lebih lengkap terutama ditinjau dari kandunga EPA dan docosahexaenoic acid (DHA) nya.  Selanjutnya rotifera yang diperkaya dengan Nannochloropsis oculata memiliki populasi yang tertinggi dibanding perlakuan lainnya. Dari penelitian ini dapat disimpulkan bahwa pengkayaan dengan EPA-TAG memiliki kualitas rotifera yang terbaik ditinjau dari sisi kelengkapan kandungan asam lemaknya. Kata kunci :  Kualitas rotifera, pengkayaan, asam eikosapentaenoik, asam dokosaheksaenoik, n3-HUFA
Growth and Feed Efficiency of Red Tilapia (Oreochromis sp.) Reared in Different Salinities Setiawati, Mia; Suprayudi, M. Agus
Jurnal Akuakultur Indonesia Vol. 2 No. 1 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.88 KB) | DOI: 10.19027/jai.2.27-30

Abstract

The objective of this research was to know the effect of salinity on the growth and utilized of feed energy by red tilapia, Oreochromis sp.  Four fishes with 4,15-4,42 g initial body weights were cultured in a 50x40x35 cm aquarium for 40 days.  Fish were fed on these diets three times a day at satiation. Dietary growth rate, feed efficiency, protein and lipid retention increased with increasing salinity (p
Dietary Phosphorus Requirement of Giant Gouramy (Osphronemus gouramy Lac.) Suprayudi, M. Agus; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 2 No. 2 (2003): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.039 KB) | DOI: 10.19027/jai.2.67-71

Abstract

This experiment was conducted to ditermined phosphorus content in the feed of giant gouramy (Osprhonemus gouramy). Four semipurified diet containing isoprotein (41,0%) and isoenergy (10.0 kcal/g protein) but different in phosphorus content were prepared. Diet containing phosphorus at the level of 0,32, 0,63, 0,89 and 1,06% respectively. Fish weighing 21-25 g/ind was stocked at a density of 4 ind./aquarium. Fish was fed at a satiation level three times daily. Fish were reared for 40 days and sampling was done in every 10 day. Result of this study shows that growth performance of fish were increase with the increasing of phosphorus level in the diet till of level (0,89%). Make up of rate phosphor more than 1,06% in the diet will negating the growth. It can be concluded that giant gouramy require phosporus at the level of 0,63-0,89% for maximal growth. Key words : Phosphor, giant gouramy, Osphronemus goruamy, mineral   ABSTRAK Penelitian ini dilakukan untuk menentukan kebutuhan ikan gurame (Osphronemus gouramy) akan mineral fosfor. Pakan yang digunakan dalam penelitian ini adalah pakan semimumi dengan kadar protein 41,0% dan rasio energi- protein 10 kkal/g protein. Pakan yang digunakan memiliki kandungan fosfor berturut-turut sebesar 0,32, 0,63, 0,89 dan 1,06%. Ikan gurame yang digunakan berbobot awal antara 21-25 g/ekor dan panjang tubuh awal 8,97-13,45 cm/ekor, dipelihara pada akuarium berukuran 50x40x35 cm dengan kepadatan 4 ekor/akuarium. Ikan diberi pakan 3 kali sehari secara satiation selama 40 hari. Setiap 10 hari sekali dilakukan penimbangan bobot tubuh ikan. Hasil penelitian menunjukkan bahwa performan pertumbuhan ikan gurame meningkat dengan meningkatknya kadar fosfor dalam makanan sampai pada tingkat 0,89%. Peningkatan kadar fosfor diatas tingkat tersebut berdampak negatif terhadap pertumbuhan. Pertumbuhan maksimal didapat pada ikan yang diberi pakan dengan kadar fosfor 0,63-1,06%. Kata kunci : Fosfor, ikan gurame, Oshpronemus gouramy, mineral.
The Improvement of Protein Retention through a Higher Utilization of Carbohydrate in the Diet with Chromium for Common Carp Cyprinus carpio Linn. Mokoginta, I.; Hapsyari, F.; Suprayudi, M. Agus
Jurnal Akuakultur Indonesia Vol. 3 No. 2 (2004): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.7 KB) | DOI: 10.19027/jai.3.37-41

Abstract

This experiment was conducted to improve the protein retention through carbohydrate efficiency in the chromium diet of common carp Cyprinus carpio. Four experimental diets with isonitrogen and isocaloric but different Cr+3 level 0,00; 1,33; 2,23 and 3,96 ppm were used in this experiment. Fish with body weight of 5,31 ± 0,02 gram were reared in aquaria (50 x 40 x 35 cm); 20 fish per aquarium. Fish fed upon the experimental diets three times daily, at satiation for 50 days. Results of this experiment showed that the protein retention could be improved by given Cr+3 in the diet. Based on the respon curve of protein retention (Yi = -1.1678X2 + 3,7223X + 24,818 ; R2 = 0,9) it was found that the optimum Cr*J in the diet was 1,59 ppm. The maximum relative growth rate was found at 2,16 ppm Cr3+ (Y2 = -14,592 X: + 63,18IX + 426,43 ; R = 0,9). The feed efficiency and the survival rate of the fish are the same between treatment (p>0.05). The protein level of the fish from treatment 3,96 ppm Cr+3 was lower than the others; the lipid level of Cr+3 diets was higher than that of non Cr+3 diet; however the carbohydrate level of fish from diet 2,23 Cr+3 was lower than the others. It was concluded that the optimum level of Cr +3 in the diet of common carp was 1,59-2,16 ppm Key words : dietary chromium, common carp, Cyprinus carpio   ABSTRAK Penelitian ini bertujuan untuk meningkatkan retensi protein dan pertumbuhan ikan mas Cyprinus carpio melalui peningkatan efisiensi karbohidrat pakan yang diberi kromium (Cr"3). Empat macam pakan dengan kadar Cr+3 yang berbeda yaitu 0,00; 1,33; 2,23 dan 3,96 ppm tetapi kadar protein dan energi yang sama digunakan pada penelitian ini. Ikan mas berbobot 5,31 ± 0,02 gram per ekor dipelihara dalam akuarium (50x40x35 cm) dengan kepadatan 20 ekor. Ikan diberi pakan tiga kali sehari secara at satiation. Pemberian pakan dilakukan selama 50 hari. Hasil penelitian memperlihatkan bahwa adanya pemberian Cr+3 dalam pakan akan mengefisienkan penggunaan karbohidrat pakan sehingga retensi protein akan meningkat pula. Retensi protein tertinggi dicapai pada kadar Cr+3 1,59 ppm (dihitung dari persamaan y, = -1,1678X2 + 3,7223X + 24,818; R2 = 0,9). Pertumbuhan relatif tertinggi dicapai pada kadar Cr+3 2,16 ppm (dihitung dari y2 = -14,592 X2 + 63.181X + 426,43; R2 = 0,9); sedangkan efiseinsi pakan dan tingkat kelangsungan hidup antar perlakuan sama (p>0,05). Kadar protein tubuh perlakuan 3,96 ppm Cr+3 relatif lebih rendah dari perlakuan lainnya. Kadar lemak tubuh ikan yang diberi Cr+3 lebih tinggi dari yang tidak diberi Cr+3; sedangkan kadar karbohidrat tubuh terendah pada perlakuan 2,23 ppm Cr+3. Secara keseluruhan dapat disimpulkan bahwa kadar Cr+3 optimum dalam pakan ikan adalah 1,59-2,16 ppm. Kata kunci : Kromium pakan. ikan mas, Cyprinus carpio
Effect of Enriched Feed by Immunostimulant on Immune Response and Growth of Humpback Grouper, Cromileptes altivelis Suprayudi, M. Agus; Indriastuti, L.; Setiawati, Mia
Jurnal Akuakultur Indonesia Vol. 5 No. 1 (2006): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.808 KB) | DOI: 10.19027/jai.5.77-86

Abstract

Humpback grouper, Cromileptes altivelis is susceptible to low quality of environmental condition so that it is vulnerable to bacterial infection.  One of the methods to solve this problem is improvement of nutrition by using immune response present in fish body.  Supplementation of immunostimulant in the diet may increase immune response of humpback grouper regarding to unsuitable environment condition and bacterial infection.  Humpback grouper in average weight of 24.6 ± 0.05 g were reared in a density of 10 fish per aquarium for 34 days.  Diet was supplemented with 4 g yeast per kg diet (diet A), 0.1 g vitamin C per kg diet (diet B), 2.5 g β-glucan (diet C), chromium-yeast 0,015 g/kg diet (diet D) and no supplementation (diet E) as control. Blood performance was analyzed after challenging test with Vibrio parahaemolyticus. The result of study indicated that immunostimulant supplementation increased leucocytes population during salinity stress test. Supplementation of chromium-yeast in the diet increased resistant of humpback grouper to Vibrio parahaemolyticus infection. Keywords: immunostimulant, humpback grouper, Cromileptes altivelis   ABSTRAK Ikan kerapu bebek, Cromileptes altivelis sangat rentan dengan kondisi lingkungan yang buruk sehingga mudah terinfeksi bakteri. Salah satu cara untuk mengatasi masalah tersebut adalah perbaikan nutrisi dengan memanfaatkan respon imun yang terdapat pada tubuh ikan. Penambahan bahan-bahan imunostimulan pada formulasi pakan buatan diharapkan dapat meningkatkan respon imunitas ikan kerapu bebek terhadap lingkungan yang tidak mendukung dan infeksi bakteri. Ikan kerapu bebek dengan bobot rata-rata 24,6 ± 0,05 g dipelihara dengan kepadatan 10 ekor/akuarium selama 34 hari. Pakan yang diberikan berupa pelet dengan tambahan beberapa bahan imunostimulan yaitu 4 g/kg ragi komersial (pakan A), 0,1 g/kg vitamin C (pakan B), 2,5 g/kg β-glukan (pakan C), kromium-yeast 0,015 g/kg (pakan D) dan tanpa penambahan bahan (pakan E) sebagai pembanding. Gambaran darah dianalisis setelah dilakukan uji tantang dengan Vibrio parahaemolyticus. Hasil penelitian menunjukkan bahwa penambahan bahan-bahan imunostimulan tersebut ternyata mampu meningkatkan populasi leukosit selama uji stres salinitas. Pemberian kromium-yeast dalam pakan meningkatkan daya tahan ikan kerapu bebek terhadap serangan Vibrio parahaemolyticus. Kata kunci: immunostimulan, kerapu bebek, Cromileptes altivelis
Co-Authors , Alimuddin , Sarmin . Ridwan . Sukenda . Vera Dewi Mulia, Troef Soemarno A Halim A. Malik Serang A. Shofy Mubarak Achmad Fauzi, Ichsan Achmad Noerkhaerin Putra Adang Saputra Adiasmara Giri, I Nyoman Afrizal Afrizal Agus Ari Afandi Agus Oman Sudrajat Ahmad Yazid Latif Aimma, Muhammad Ariful Akhmad Baihaqi Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin alimuddin alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Amelia Oktaviani, Amelia Ani Rusilowati Apriana Vinasyiam Aprilianti Pratiwi Arief Boediono Aris Sudiyanto Aris Tri Wahyudi Arradini, Dewi Artin Indrayati Asda Laining Asda Laining Asih Wahyuningsih Azmi Afriansyah Benny Heltonika Besral . Burhanudin Faisal, Burhanudin D. Jusadi Dairun, Suclyadi DARMAWAN SETIA BUDI Darsiani Darsiani darsiani, Darsiani Deddy Yaniharto Deddy Yaniharto DEDI JUSADI Dedi Yaniharto dedy yaniharto Denny Wahyudi Dewi Rosanti DIAH AYU SATYARI UTAMI Diamahesa, Wastu Ayu Dian Mutiara Didi Humaedi Yusuf, Didi Humaedi Dinamella Wahjuningrum Dini Harianto Dodi Hermawan Duwi Nuvitalia E. Mursitorini Eddy Supriyono Edy Marsudi Elas, Putri Elvianita, Diana Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja Endang Wuryandini, Endang Endriatmo Soetarto Ernawati Saptaningrum F. Hapsyari Faiqotul Himmah, Maihardiyanti Fatkhullah, Faiz Karim Fatma Hajiali Fauzan Fredinan Yulianda Gadis Sri Haryani Gebbie Edriani Ghina Raudathul Jannah Giri Maruto Darmawangsa, Giri Maruto Gumelar, Muhammad Raditya Gusriani Hadi Pratama, Ricky Harton Arfah Hasan Abidin Hasan Nasrullah Hasrah Hasrah Hema Widiawati Hendriana, Andri Husnidar Husnidar I Dewa Putu Oka Suardi I Gede Setiawan Adi Putra I Gusti Agung Gede Wiadnyana I GUSTI AGUNG GEDE WIADNYANA . I Gusti Ayu Rai I Gusti Ayu Rai I MADE ARTIKA I Made Artika I Nengah Suka Widana I Wayan Budiyasa I Wayan Nurjaya I Wayan Suanda I. Mokoginta Ichsan Achmad Fauzi Ichsan Achmad Fauzi Iis Diatin Imah, Nenden Imaroh Izzatun Nisa Imlani, Ainulyakin Hasan Inarto, Hendri ING MOKOGINTA Iqbal Kurniawinata, Mohamad Ira Akhdiana Irmawati Irvan Faizal Irwan Irwan Irzal Effendi Ismarica, Ismarica Iwandikasyah Putra Joko Tri Atmojo Jr., Muhammad Zairin Juli Ekasari Julie Ekasari Jullie Ekasari KATSUYUKI HAMASAKI KATSUYUKI HAMASAKI KETUT BUDI SUSRUSA Khumaedi - Komang Adi Wika Wiguna Kusriyati Kusriyati L. Indriastuti Latifah Kosim Darusman Luh Putu Ratna Sundari M. Ikhsan M. Setiawati M. Tri Djoko Sunarno M. Zairin Junior Mas Tri Djoko Sunarno Masrunik, Endah Meilisza, Nina Meritha, Wellya Wichi Mia Setiawati Miftakhul Huda, Anam Moh. Yamin Darsyah Mudinillah, Adam Muhaimin Hamzah Muhammad Jailani Muhammad Junior Zairin Muhammad Khafid Muhammad Roikhan Amanullah Muhammad Safir Muhammad Zairin Jr Muhammad Zairin JR Muhammad Zairin Jr Muhammad Zairin Jr MUHAMMAD ZAIRIN Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Mulyasari Mulyasari Munawar Khalil Muslim Muslim Muzahar Nadrah Ngurah Ayu NM Niagara, Niagara Nina Meilisza Noviani, Doris Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nur Bambang Priyo Utomo Nuraini Annisa, Nuraini Nuril Farizah Odang Carman Otie Dylan Soebhakti Hasan Padlilah, Rahmi Padlillah, Rahmi Pattipeilohy, Christian Ernsz Pika Lusi Andriati Ponda Sari Styawati PRIMA AYU RIZQI MAHANANI, PRIMA AYU RIZQI R Rifai R. Sutajaya Raditya Gumelar, Muhammad Rahmawati, Rahmawati Rahmayanti, Yessy Rahminiwati, Min Rakhmawati, Rakhmawati, Ria Septy Anggraini, Ria Septy Ricky Ramadhan Ridhia Putri RIDWAN AFFANDI Riki Agustiono Rina Hirnawati Ririn Ariyanti Ririn Ariyanti, Ririn Rita Hayati Rizky Nafaida Sabrin, Gusriani Samalei, Ermianus Sarwititi Sarwoprasojdo, Sarwititi Senja, Reza Karunia Setyorini, Catur Sintia Valinta Siti Komariyah Sofyan Sauri Sri Nuryati Sri Nuryati Suardi Laheng Suci Antoro Suci antoro Suclyadi Dairun Supatmin, Supatmin Supriyadi Hari Respati Suryadi Saputra Suryani Kusumaningtyas, Rina Dwi Susilawati ikip pgri semarang T. Makmur Talita Shofa Adestia Tatag Budiardi Tawahusna Tawahusna Tiara Ramadhani Titin Kurniasih TOSHIO TAKEUCHI TOSHIO TAKEUCHI Toto Sunarto Tulas Aprilia Upmal Deswira Utomo, Nurbambang Priyo Vera Fitria Vernandes Evenus Bulu Wahyudi, Imam Tri Wasmen Manalu Wida Lesmanawati WIDANARNI WIDANARNI Widiyanto, Aris Wijianti, Hani Wijiwinarsih, Amallia Wika Wiguna, Komang Adi Winda Styani Irawan, Winda Styani Wiyoto Wiyoto Y. Hadiroseyani yayat Nur hayati Yogo Tri Saloko Yudha Trinoegraha Adiputra Yudha Trinoegraha Adiputra Yusrizal Yusrizal Yutaka Haga Zairin Jr., Muhammad Zinatul Hayati