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GENETIC DIVERSITY OF INDONESIAN SHALLOTS BASED ON BULB-TUNIC PATTERNS AND MORPHOLOGICAL CHARACTERS Lina Herlina; Reflinur Reflinur; Sobir Sobir; Awang Maharijaya; Suryo Wiyono; Bonjok Istiaji
Indonesian Journal of Agricultural Science Vol 20, No 1 (2019): June 2019
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v20n1.2019.p19-28

Abstract

Variation within bulb tunics has been used to determine the genetic diversity in Allium species, including shallots. However, no such study has been reported for shallots of Indonesia. The study aimed to analyze the genetic diversity of the Indonesian shallots based on the bulb-tunic patterns. Thirty-five shallot genotypes from main production centers in Indonesia were used. The ultrasculptures of the bulb tunics were examined by light microscopy, including the inner surface and cell shape patterns of the bulb tunics. The phenotypic data, i.e. quantitative and qualitative traits were subjected to the descriptive statistics, principal component, correlation, regression, and clustering analyses. The results showed that the bulb-tunic cell patterns were varied, which shared almost identical with 13 Allium species. Total bulb weight per genotype showed the greatest variation (cv = 89.10%) and significant correlation with bulb weight per plant (r = 0.773). The principle component analyses showed the cumulative proportion of 78% of the total morphological variation in all shallot genotypes. Based on clustering analysis, the genetic variation of Indonesian shallots are grouped into twelve clusters with 50% genetic similarity. The study indicates that Indonesian shallots are genetically varied and could be useful for further utilization in their genetic improvement program.
Identifikasi Isolat Khamir Berpotensi sebagai Agens Antagonis dan Uji Produksi Toksin Hemolisin Sri Hartati; Suryo Wiyono; Sri Hendrastuti Hidayat; Meity Suradji Sinaga
Agrikultura Vol 32, No 2 (2021): Agustus, 2021
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v32i2.33849

Abstract

Identifikasi khamir dapat dilakukan secara konvensional maupun molekuler. Identifikasi secara konvensional membutuhkan waktu yang lama dan interpretasi hasilnya seringkali bersifat subyektif. Sementara identifikasi khamir dengan metode molekuler dapat memberikan hasil yang lebih akurat dan cepat. Khamir yang berperan sebagai agens antagonis harus aman terhadap organisme nontarget agar dapat diaplikasikan di lapangan. Penelitian ini bertujuan untuk mengidentifikasi isolat-isolat khamir berpotensi antagonis dengan metode molekuler dan mengetahui kemampuan khamir dalam menghasilkan hemolisin sebagai salah satu indikator potensi resiko terhadap mamalia. Identifikasi dan pengujian kemampuan khamir dalam menghasilkan hemolisin dilakukan pada 15 isolat khamir berpotensi antagonis terhadap patogen antraknosa cabai (Colletotrichum acutatum). Identifikasi khamir dilakukan secara molekuler dengan PCR menggunakan primer ITS1 dan ITS4. Penyediaan khamir menggunakan mediaYeast Malt Extract Broth (YMB) dan Potato Dextrose Agar (PDA). Pengujian kemampuan khamir dalam menghasilkan hemolisin menggunakan media blood agar base (Oxoid CM55) ditambah darah domba 5%. Hasil identifikasi menunjukkan bahwa isolat khamir dapat teramplifikasi dengan primer ITS1 dan ITS4 dengan ukuran  fragmen   produk   antara  500-800 pb. Hasil analisis sekuensing didapatkan 6 spesies khamir yaitu Candida tropicalis, Rhodotorula minuta, Aureobasidium pullulans, Pseudozyma hubeiensis, Pseudozyma aphidis, dan Pseudozyma shanxiensis. Uji kemampuan khamir dalam menghasilkan hemolisin menunjukkan bahwa seluruh khamir yang diuji tidak menghasilkan toksin hemolisin sehingga diduga isolat-isolat tersebut tidak patogenik terhadap manusia.
Keefektifan Bahan Pencuci dan Pencegah Penyakit Terhadap Kualitas Buah Mangga CV. Gedong Gincu dan Arumanis (The Effectiveness of Washing Materials and Disease Protecting Agent on the Quality of Mango Fruit cv. Gedong Gincu and Arumanis) Ahmad Sutopo; Roedhy Poerwanto; Suryo Wiyono
Jurnal Hortikultura Vol 27, No 2 (2017): Desember 2017
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v27n2.2017.p253-260

Abstract

Mangga merupakan salah satu komoditas buah tropis di Indonesia yang mempunyai peluang besar untuk pasar domestik dan juga ekspor. Namun, kualitas buah mangga masih memiliki banyak permasalahan. Salah satunya adalah getah yang mengotori kulit buah mangga. Pada saat tangkai buah mangga rusak, getah menyebar pada kulit buah yang menyebabkan kerusakan kulit dan serangan penyakit. Penelitian bertujuan mendapatkan bahan pencuci dan pencegah penyakit yang efektif terhadap kualitas buah mangga cv. Gedong Gincu dan Arumanis. Buah mangga dipanen di kebun petani di Cirebon, Jawa Barat dan Probolinggo, Jawa Timur. Pengamatan dilakukan di Laboratorium Pascapanen Institut Pertanian Bogor pada bulan November 2013 sampai Januari 2014. Penelitian menggunakan rancangan acak lengkap nonfaktorial yang terdiri atas sembilan perlakuan, yaitu air (kontrol) (P0T0), air (kontrol) + fungisida (P0T1), air (kontrol) + khamir Cryptococcus albidus dengan konsentrasi 5 x 104 sel/ml (P0T2), deterjen 1% + air (kontrol) (P1T0), deterjen 1% + fungisida (P1T1), deterjen 1% + khamir C. albidus (P1T2), deterjen 1% + Ca(OH)2 0,5% + air (kontrol) (P2T0), deterjen 1% + Ca(OH)2 0,5% + fungisida (P2T1), dan deterjen 1% + Ca(OH)2 0,5% + khamir C. albidus (P2T2). Hasil penelitian menunjukkan bahwa deterjen 1% + Ca(OH)2 0,5% + khamir C. albidus 5 x 104 sel/ml, dan deterjen 1% + Ca(OH)2 0,5% + fungisida 0,025% yang paling efektif menghilangkan getah, mengurangi luka bakar, bintik lentisel, mencegah terjadinya kerusakan penyakit antraknosa dan busuk pangkal buah dibandingkan dengan perlakuan kontrol pada kultivar Gedong Gincu dan Arumanis.KeywordsAntraknosa; Busuk pangkal buah; Getah; Luka bakar; KhamirAbstractMango is one of the commodity of tropical fruit in Indonesia which has a great opportunities for domestic market and also export. However, quality of mango still has many problems in Indonesia. One of them is the sap contaminating the skin of mango fruit. When the stem of mango fruit is broken, the sap oozes out spreading over the fruit skin causes serious skin damages and attack of disease. The aim of this study was to determine the effectiveness of washing materials and disease protecting agent on the quality of mango fruit cv. Gedong Gincu and Arumanis. Mango fruit was harvested in farmer garden in Cirebon, West Java and Probolinggo, East Java and observation was conducted in Postharvest Laboratory of Bogor Agricultural University in November 2013 to January 2014. The experiment was designed in a completely randomized design nonfactorial that consist of nine treatments: water (control) (P0T0), water (control) + fungicide (P0T1), water (control) + yeast Cryptococcus albidus with concentration 5 g/liter 5 x 104 cell/ml (P0T2), detergent 1% + water (control) (P1T0), detergent 1% + fungicide (P1T2), detergent 1% + yeast C. albidus (P1T0), detergent 1% + Ca(OH)2 0.5% + water (control) (P2T0), detergent 1% + Ca(OH)2 0.5% + fungicide (P2T1), and detergent 1% + Ca(OH)2 0.5% + yeast C. albidus (P2T1).The result showed that the treatment of detergent 1% + Ca(OH)2 0.5% + yeast C. albidus and detergent 1% + Ca(OH)2 0.5% + fungicides 0.025 was effective to removing of sap, less of sap burn injury, lenticel spoting, protecting agent of anthracnose disease and stem end rot as compared to control on mango fruit cv. Gedong Gincu and Arumanis.
Genetic Diversity Analysis Using Resistance Gene Analog-Based Markers to Support Morphological Characterization of Shallots Lina Herlina; Reflinur Reflinur; Kristianto Nugroho; Rerenstradika T. Terryana; Sobir Sobir; Awang Maharijaya; Suryo Wiyono
Jurnal AgroBiogen Vol 14, No 2 (2018): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n2.2018.p65-74

Abstract

Shallot (Allium cepa var. aggregatum) is one of the most important vegetable crops grown in Indonesia. The limited knowledge available on the genetic diversity and the threat of plant disease have been major problems to maintain high shallot production in Indonesia. Development of molecular markers linked to disease resistance is required for molecular breeding activity in this crop. This study aimed to assess the genetic diversity at conserved domain of resistance gene analog (RGA) in a set of 36 Indonesian shallot genotypes to complement morphological characterization. Twelve morphological and fifteen molecular markers traits were investigated in an attempt to characterize and to discriminate the Indonesian shallots genotypes. Characterization at orphological level indicated that phenotypic variance was highest for total bulb weight (TWB, cv = 99.39%) and the least for the plant height (PH, cv = 28.16%). The correlation analysis between traits showed that TWB and number of bulb (NB), TWB and bulb weight per plant (WB), NB and WB, and WB and PH were positively correlated. Molecular analysis revealed a total of 1,512 alleles with an average of 1.946 alleles per locus. The Polymorphism Information Content (PIC) values ranged from 0.253 to 0.676 and six out of 15 RGA markers were highly informative with PIC values ≥0.50. Based on cluster analysis, the 36 Indonesian shallot genotypes were clearly discriminated into six major groups. These results revealed that the RGA-based markers could support the morphological characterization in evaluating the genetic diversity of shallots. 
Resistance Responses of 35 Watermelon Genotypes to Three Isolates of Fusarium oxysporum f. sp. niveum Nazly Aswani; Suryo Wiyono; Sobir Sobir
Jurnal AgroBiogen Vol 17, No 2 (2021): DESEMBER
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v17n2.2021.p63-74

Abstract

Fusarium wilt caused by Fusarium oxysporum f. sp. niveum (Fon) is one of main diseases of watermelon. There have been very limited studies that tested watermelon genotypes to more than one isolates of Fon in Indonesia. This research aimed to determine the resistance of 35 watermelon genotypes to three Fon isolates taken from three different areas in Indonesia. Incubation period (IP) and disease index (DI) of the 35 watermelon genotypes were determined against three Fon isolates collected from Karawang (FK), Lampung (FL), and Purwakarta (FP). The experiment was arranged using a Completely Randomized Design with two replications. DI showed that six watermelon genotypes, i.e. New Hope, Sky Mountain, Southern Light, Super Sweet 66, Uranus, and Yellow Baby demonstrated moderate resistance to resistance phenotypes to all tested Fon isolates. IP and percentage of symptomatic plants (PSP) showed different responses among genotypes either to the same or to different isolates. Genotype New Orchid, for example, showed 57.14% symptomatic plants in less than 10 days after inoculation (DAI) when tested with FL isolate. Meanwhile, when tested with the same isolate, genotype New Dragon showed only 6.67% symptomatic plants in more than 23 DAI. The result of this study indicated that watermelon genotypes showing resistant to all tested isolates should be useful for breeding program to develop watermelon lines with broader resistance spectrum against Fon pathogen. The resistance genotypes selected should also demonstrate good agronomic performances and high yield to be considered as a new watermelon variety.
Genetic Diversity Analysis of 41 Chili Pepper Genotypes (Capsicum annuum L.) Based on SSR Markers Wartono Wartono; Suryo Wiyono; Muhamad Syukur; Giyanto Giyanto; Kristianto Nugroho; Puji Lestari
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p65-74

Abstract

Chili (Capsicum annuum L.) is an important horticultural crop which possesses high genetic diversity. Their genetic diversity needs to be assessed through molecular marker-based analysis to facilitate chili breeding scheme. The objective of the study was to analyze the genetic diversity of 41 chili genotypes using Simple Sequence Repeat (SSR) markers. Eleven SSR markers were used to amplify all genotypes through PCR technique, visualized on Polyacrylamide Gel Electrophoresis (PAGE). The data were analyzed using NTSYSpc version 2.1 and PowerMarker version 3.25 softwares. Results showed that the average of SSR alleles was 7.9 with a range of 4-11 alleles per marker. All SSR markers showed PIC value >0.5, indicating their suitable use for chili genetic studies. Cluster analysis of 41 genotypes generated three main groups according to coefficient similarity of 77.4%. The genetic clusters appeared as reflection of the genetic background of each genotype. In addition, the SSR markers used can identify potential parent as indicated by the genetic distance between genotypes. The highest genetic diversity (0.698) was demonstrated by combinations of Yuni × Ayesha, Yuni × Ayesha 2, and Yuni × Namira. The estimated genetic diversity produced by SSR markers in this study should be useful as guidance for chili breeders to select suitable parents for new cultivar development for high productivity and resistance to diseases.
Skrining Bakteri Selulolitik Asal Saluran Pencernaan Rayap Untuk Mendekomposisi Tunggul Karet Silviana Arsyad; Suryo Wiyono; Elis Nina Herliyana
Jurnal Silvikultur Tropika Vol. 9 No. 3 (2018): Jurnal Silvikultur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.9.3.217-222

Abstract

The rubber tree stump is a source of white root fungus inoculums and as a source of infection that causes the death of rubber plant. The emergence of this disease is closely related to the cleanliness of the land such as leftover trees or stumps, shrubs and bushes which stacked or still in the ground. One of the symbionts organisms found in gut of termite and play a role in the decomposition of cellulose, are bacteria. Termite are social insects that are efficiently decompose lignocelluloses with the aid of their associated microbial symbionts located in termite gut. The purpose of this study was to obtain cellulolytic bacterial isolates are derived from gut of termite and getting an cellulolytic which showed the best ability in decomposing rubber tree stumps. The result termite samples from oil palm, rubber, and dry wood contain cellulolytic bacteria characterized by clear zones around bacterial colonies. Bacterial isolates showing the ability to degrade cellulose are 31 isolates and five isolates are safe for plants, animals, or mammals. The three bacterial isolates (NK 4, NS 4, and NS 5) used in the test on rubber tree stumps able to decompose rubber tree stump. Bacterial isolates were each obtained NK 4 13.52%, NS 4 18.40% and NS 5 17.88%.Keywords: bacteria cellulolytic, rubber tree stumps, termite
POTENSI KHAMIR UNTUK MENGENDALIKAN PENYAKIT ANTRAKNOSA (Colletotrichum acutatum L.) PADA TANAMAN CABAI (Potency of Endophytic Fungi and Yeast as Biological Control to Pepper Anthracnose (Colletotorichum acutatum L.) Weni Wilia; . Widodo; Suryo Wiyono
Bioplantae Vol. 1 No. 4 (2012): Bioplantae
Publisher : Bioplantae

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Anthracnose caused by Colletotrichum actuate is one of devastated disease ofpepper in Indonesia that has great impact on yield loss. Most of farmers usefungicides as controller for this disease. Application of biocontrol agentscould be one ways to control anthracnose. Beneficial microorganism such asyeast is able to be promising biocontrol agents of some pathogens, includingColletotrichum. The aim of this research was to isolate yeast from fruit andbranch as biocontrol agents for pepper anthracnose. The research results 9isolates of yeasts that were further tested against anthracnose. Isolates ofyeasts were identified as Cryptococcus terreus, C. abides var. aerius IPB 1,and C. abides var. aerius IPB 2, Candida Edam. Other isolates coded asCBN, CBM, CBK, CBF, and CBR were not identified yet. Five of potentialyeasts, Cryptococcus terreus, C. albidus var aerius IPB 1, C. albidus varaerius IPB 2, Candida edax, and unidentified yeast CBN showed directantagonist mechanism. Among of potential yeasts isolated, CBN showedability to reduce incidence of disease up to of 87.50%.Key word: Pepper, antrachnose, C. acutatum, yeast
EKSPLORASI CENDAWAN ENDOFIT DARI TANAMAN CABAI YANG BERPOTENSI SEBAGAI AGENS BIOKONTROL PENYAKIT ANTRAKNOSA (Colletotrichum acutatum L.) (Exploration of Endophytic Fungi from Pepper as Biological Control Agents of Anthracnose (Colletotrichum acutatum L.) Weni Wilia; . Widodo; Suryo Wiyono
Bioplantae Vol. 2 No. 1 (2013)
Publisher : Bioplantae

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Anthracnose caused by Colletotrichum acutatum is one of devastated disease of pepper in Indonesia and has great impact on yield loss. Most of farmers use fungicides as controller for this disease. Application of biocontrol agents could be solution to control anthracnose. Beneficial microorganism, such as endophytic fungi are able to be promising biocontrol agents of some pathogens, including Colletotrichum acutatum. The aim of this research was to isolate endophytic fungi from fruit and branch as biocontrol agents for pepper anthracnose. The research resulted 11 isolates of endophytic fungi that were further tested against anthracnose. Isolates of endophytic fungi were identified as steril hifa hitam 1, steril hifa hitam 2, steril hifa 1, steril hifa 2, Nigrospora sp., Coniotyrium sp., Cylindrocarpon sp., Paecilomyces sp. The 3 of other isolates (isolat buah 5, isolat buah 1dan isolat batang 24) were not identified yet.   Keyword : Pepper, antrachnose, C. acutatum, endophytic fungi
Skrining Bakteri Dekomposer Sebagai Penghilang Bau Kotoran Ayam Nika Ropiatningsuari; Suryo Wiyono; Suryahadi Suryahadi
Bumi Lestari Journal of Environment Vol 18 No 1 (2018)
Publisher : Environmental Research Center (PPLH) of Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/blje.2018.v18.i01.p03

Abstract

The decomposition of chicken excreta produce odorous gases, that case environmental pollution. One of alternative technique to reduce the odorous gases is by applying specific bacteria. The aim of this study was to obtain bacterial isolates that capable of reducing NH3 and H2S production and odors. Decomposer bacterial candidates were isolated from chicken manure, guano, and peat soil. Selection of isolates of oxidizing bacteria is carried out using selective media. All isolates that were found from chicken manure, guano, and peat soil tested for hypersensitive reaction on tobacco and hemolysis on blood agar. The isolated bacteria that showed negative HR and HL responses than used for a further experiment. Five isolates of bacteria which reduce odors based on organoleptic test were WiK 15, TnK 7, WiGu 11, CGu 7 and MaGa 5. NH3 and H2S from decomposition chicken excreta were analyzed by spectrophotometric and colorimetric method. The average of total NH3 production from seven days observation showed ranged level from 1.09 ppm to 1.77 ppm, while total H2S gas production ranged from 15.05 to 16.57 ppm. Bacterial isolate CGU 7 showed make lowest total production of NH3.
Co-Authors . Haryanto . Rifka . Widodo . Widodo . Widodo Abdjad Asih Nawangsih Abdul Munif ABDUL MUNIF Adi Setyo Purnomo Agus Purwito Ahmad Fauzi Ridwan AHMAD JUNAEDI Ahmad Junaedy Ahmad Sutopo ALI NURMANSYAH Ali Wafa Aloysius Rusae Amrullah, Rizki Abi Anas Dinurrohman Susila Andika Septiana Suryaningsih Andriani, Desta Angelica Nabilla Ahdy Anisa Nurkasanah Anjar Kartika Antonius Suwanto ARI SUSILOWATI Arif Ravi Wibowo Aris Tri Wahyudi Asniah Asniah Astuti Kurnianingsih Atit Kanti Atit Kanti Awang Maharijaya Ayu Rahayu Baskoro Sugeng Wibowo Bayu Aji Krisandi Bonjok Istiaji Burhanudin Burhanudin Cahyani, Gesa Nur DADANG DADANG DAMAYANTI BUCHORI Daniel Happy Putra Darni Rambu D. Siala Dedi Fardiaz Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga DEWI SARTIAMI Dewi Sukma Dhany Nukhaizal Azkiya DWI ANDREAS SANTOSA Dwi Astuti DWI SUGIPRIHATINI Dyah Harianti Purnomo Efi Toding Tondok Eka Maulidiya, Sherly Eka Wijayanti Elis Nina Herliyana ENDANG MURNIATI Eryna Elfasari Rangkuti Evan P. Ramdan Fadillah, Arvin Muhammad Fakhrusy Zakariyya Fany Juliarti Panjaitan Fariyanti, Anna Fatah, Fadillah Abdul Fazad, M. Haikhal Febbiyanti, Tri Rapani Firman Hidayat Fitri Kemala Sandra Fitriansyah, Muhammad Ramdhani Giyanto Giyanto Giyanto Giyanto Giyanto Giyanto, Giyanto Halimah, Adedila Hanif, Andini HAPPY WIDIASTUTI Hari Priwiratama Harti, Heri Hendrastuti Hidayat, Sri Hendro Nurhadi Heny Setiyowati Heri Harti Hermanu Triwidodo Hidayah, Zahra Nurul Hidayat , Sri Hendrastuti I MADE ARTIKA I Made Sudiana I Made Sudiana I Nyoman Sumerta Idris Idris IKA DAMAYANTI Iman, Muhammad Ilham Nur Imaniasita, Vidya Indrapaksi, Tengku Ikhwan Alfajri Iskandar Z. Siregar Islah Hayati Ita Aprilia Ivone Oley Sumarauw Joni Hidayat, Joni Juang Gema Kartika Kartini Budiastuti Ketty Suketi Khairani Khairani Khairina, Winda Hidayatul Khamidi, Thamrin Khoirotul Afifah Kikin H Mutaqin Kristianto Nugroho Kristianto Nugroho Kudang Boro Seminar Kusuma Darma Kusuma Darma Kusumah, Yayi Munara Lestari, Mutiara Dwi Lilik Retnowati Lina Herlina Lina Herlina Listihani, Listihani M. Miftahudin Masrukhin Masrukhin Mei Lita Fitriani Meity S Sinaga Meity S. Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Memen Surahman Mohamad Rahmad Suhartanto Mufidah, Iftin Farekana Muhammad Arief Munady Muhammad Lukman Hakim, Muhammad Lukman Muhammad Miftahudin Nanta, Wahyu Ridwan Napiudin Nazly Aswani Neo Endra Lelana nFN Khaerati Nika Ropiatningsuari Nur Alfi Saryanah Nur Khoiriyah Nurmala, Ayu Aulya Nurul Khumaida Octaviani, Wulan Nur Parida, Ida Phor Bho Ayuwati Pratama, Silvia Abdi Pratiwi, Astika Widhi Pratiwi, Tasya Resa Priyo Cahyono Puji Lestari Purnomo, Dyah Hariyanti Purwono Purwono, Purwono Puspitaningtyas, Khasanah Putri, Lystiana Dewi Putro, Herdayanto Sulistyo RAHAYU WIDYASTUTI Ramdan, Evan Purnama Reflinur Reflinur Reflinur Reflinur Remaja Sitepu Rerenstradika T. Terryana Riana Jumawati Rika Estria Gurusinga Rina Mardiana Rizki Abi Amrullah Roedhy Poerwanto Rohmah, Alya Awinatul Roza Yunita Rustam, Rustam Rustiani, Ummu S. Said Abdullah Saiful Akhyar Lubis Saputra, Bubun Afif Hidayat Aziz Sari Nurulita Sarwititi Sarwoprasodjo Satya Nugroho Sherly Eka Maulidiya Silviana Arsyad Sima Elpani Siregar, Amelia Nani Sitti Eha Faihah Sitti Fadhilah Slamet Susanto Sobir Sobir SRI FATMAWATI Sri Hartati Sri Hartati Sri Hartati SRI HENDRASTUTI HIDAYAT Sri Wening Stephanie Stephanie Sudarsono Sudirman Yahya Sudirman Yahya Sudirman Yahya Sudradjat Suhadi, Octen Sukmana, Andra Sahab Surya Rosa Putra, Surya Rosa Suryahadi Suryahadi Susanna Susanna SUSILOWATI1 SUSILOWATI1 Suwarno . Swastiko Priyambodo, Swastiko Tamrin Khamidi Tamrin Khamidi Tamrin Khamidi Tamrin Khamidi Teguh Iman Santoso Teguh Iman Santoso Titiek Siti Yuliani Toding Tondok, Efi Toga Pangihotan Napitupulu TRI ASMIRA DAMAYANTI Tri Rapani Febbiyanti Tri Rapani Febbiyanti, Tri Rapani Trikoesoemaningtyas Ummu S. Rustiani Ummu Salamah Rustiani Utami, Aditya Dyah UTUT WIDYASTUTI Vinsen Willi Wardhana Wahyu, Bonny Poernomo Wartono Wartono Wati, Cheppy Wawan Setiawan Weni Wilia Widhayanti, Nur Ari Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widya Sari Winarso D. Widodo Winda Ika Susanti Windah Meilanda Zulmi Yayu Siti Nurhasanah Yohana C Sulistyaningsih Yohana C Sulistyaningsih Yuli Fitriati Yuliawati YULIN LESTARI Yundari, Yundari Yuyun Andriyani