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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Silvikultur Tropika (JST) Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Bioplante Bumi Lestari Agrikultura Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Keteknikan Pertanian Jurnal Penelitian Hutan Tanaman Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Perlindungan Tanaman Indonesia BERITA BIOLOGI Jurnal Penelitian Karet JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Penelitian Kelapa Sawit AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Hexagro Agro Bali: Agricultural Journal Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Jurnal Bioindustri Jurnal Penelitian Tanaman Industri (Littri) Jurnal Tanah dan Iklim Biofarm : Jurnal Ilmiah Pertanian Jurnal Risalah Kebijakan Pertanian dan Lingkungan Jurnal Penyuluhan Pertanian Proceedings Series on Physical & Formal Sciences Jurnal Pusat Inovasi Masyarakat PERTANIAN TROPIK Sewagati Policy Brief Pertanian, Kelautan, dan Biosains Tropika Journal of Bioindustry
Suryo Wiyono
Departemen Proteksi Tanaman, Fakultas Pertanian Insitut Pertanian Bogor Jl. Kamper, Kampus IPB Dramaga, Bogor 16680
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Pemanfaatan Khamir Antagonis untuk Memperpanjang Umur Simpan dan Mengendalikan Penyakit Antraknosa Buah Pepaya Lestari, Mutiara Dwi; Suketi, Ketty; Widodo, Winarso Drajad; Wiyono, Suryo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 48 No. 3 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (356.533 KB) | DOI: 10.24831/jai.v48i3.32167

Abstract

Pepaya adalah buah klimakterik yang memiliki umur simpan pendek dan potensi gangguan penyakit antraknosa pada saat tahap pascapanen yang disebabkan oleh patogen Colletotrichum gloeosporioides. Tujuan dari penelitian ini adalah mengkaji keefektifan beberapa spesies khamir antagonis untuk memperpanjang umur simpan dan mengendalikan penyakit antraknosa pada pascapanen buah pepaya Callina. Penelitian ini dilaksanakan di kebun pepaya Desa Kanaga, Kabupaten Lebak, Banten pada November 2018 sampai Juli 2019 dan Laboratorium Pascapanen AGH, IPB pada bulan Maret sampai dengan Juli 2019. Penelitian ini menggunakan rancangan kelompok lengkap teracak dengan perlakuan lima jenis khamir yaitu Cryptococcus albidus Yp, Aureobasidium pullulans Dmg 30 DEP, Rhodotorula minuta Dmg 16 BEP, Candida tropicalis Lm 13 BE, dan Pseudozyma hubeiensis Dmg 18 BEP, dan sebagai pembanding perlakuan fungisida berbahan aktif azoksistrobin serta tanpa perlakuan sebagai kontrol. Hasil penelitian menunjukkan penggunaan khamir Candida tropicalis, Aureobasidium pullulans, dan Cryptococcus albidus dapat memperpanjang umur simpan buah pepaya Callina masing-masing 12.6, 12.4, dan 12.2 hari, lebih lama dibandingkan kontrol yang hanya 7 hari, dan efektif mengendalikan penyakit antraknosa dengan tingkat penekanan secara berturut-turut 58.33%, 54.17%, dan 50.00%, selama penyimpanan. Penggunaan khamir antagonis tidak mempengaruhi mutu fisik dan kimia buah pepaya Callina. Kata kunci: buah klimakterik, Colletotrichum gloeosporioides, pascapanen
Determination of anthracnose (Colletotrichum gloeosporioides) resistance group in shallot (Allium cepa var. aggregatum) Rizki Abi Amrullah; Maharijaya, Awang; Purwito, Agus; Wiyono, Suryo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 3 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v51i3.47429

Abstract

Shallot anthracnose, caused by Colletotrichum gloeosporioides, is a devastating disease in a tropical country with high humidity and rainfall. Chemical control of anthracnose is neither economical nor eco-friendly, and genetic resistance is considered an efficient management method. This study aimed to determine the resistance groups of several shallot varieties and predict resistance heritability characteristics. In this study, a total of 13 Indonesian shallot varieties were evaluated for anthracnose resistance and separated into two groups, resistance and susceptible, based on K-means clustering developed by using disease resistance/susceptibility characteristics such as incubation period, disease incidence, disease severity, and spot diameter. The results indicate that the Agrihorti, Maja Cipanas, Batu Ijo, and Rubaru varieties were identified as resistant groups based on lower disease severity and incidence, smaller spot diameter, and longer incubation period. Maja Cipanas and Rubaru were more consistent in all variables, which is recommended as a source of genetic resistance genotypes. On the other hand, the Biru Lancor, Bima Brebes, Srikayang, Violetta, Slupu Merah, Pancasona, Sakato, Katumi, and Kuning varieties were identified as susceptible groups based on high disease severity and incidence, large spot diameter, and short incubation period. Keywords: susceptibility characteristics, genetic resistance, k-means clustering, disease severity
Pattern distribution and epidemic factors of stem canker disease caused by Neoscytalidium dimidiatum on pitahaya in Banyuwangi Regency, East Java, Indonesia Sukmana, Andra Sahab; Tondok, Efi Toding; Wiyono, Suryo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12646-58

Abstract

Neoscytalidium dimidiatum is an airborne pathogen that causes stem canker disease, characterized by small brown spots surrounded by a yellow halo on the plant stem. These spots expand and darken from brown to dark brown and eventually black. In addition to attacking the plant stem, this disease can also infect the fruit. This research aims to understand the distribution pattern model of stem canker disease and to identify the factors contributing to the epidemic caused by N. dimidiatum. The study was conducted through the collection of secondary data, field observations of disease severity, assessment of farmers’ cultivation practices via questionnaires, isolation of phyllosphere and rhizosphere microbes, and soil chemical analysis. The results showed that this pathogen initially exhibits a random distribution pattern but later develops into infection patches with two possible dissemination modes based on the observed distribution pattern: airborne transmission and vegetative planting materials. Environmental factors influencing the stem canker epidemic include rainfall exceeding 98 mm/month, a temperature of 27 ºC, and humidity of 78.3%. Biotically, the diversity and abundance of phylloplane and rhizosphere microbes in low-severity areas are higher than in high-severity areas. From the abiotic aspect of soil chemistry, the elements P, Mg, Fe, and total N are associated with reduced stem canker intensity. Four aspects of cultivation techniques are closely related to the human role in the formation of the stem canker epidemic: the use of phytohormones, the control methods employed, the choice of pitahaya varieties, and yield losses.
Powder Formulation of Antagonists Yeasts, Cryptococcus albidus and Cryptococcus terreus as Biofungicides Wiyono, Suryo
BIOTROPIA Vol. 20 No. 1 (2013): BIOTROPIA Vol. 20 No. 1 June 2013
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2013.20.1.264

Abstract

The research has following objectives: 1) to investigate compatibility of yeasts antagonist  C. albidus and C. terreus, 2) to obtain suitable carrier  in powder formulation for those two yeasts,  3) to get appropriate formulation additives for those yeasts,  and 4) to obtain optimum powder formulation consisting yeasts, carrier materials and additives.  Compatibility of C. albidus and  C. terreus  was tested through bio-assay  against Lasiodiplodia theobromae on detached banana fruit  and Alternaria solani on detached tomato leaves.  Compatibility was indicated by no reduction of antagonistic activity.  Benomyl-resistant mutant of C. albidus  and cycloheximide-resistant mutant of  C. terreus were mixed with sterilized tapioca, talc and kaolin  to get initial yeasts density of  8.5 log cfu/g and water content of 15%,  then  packed by plastic bag and stored under room temperature. Survival of formulated yeasts was assessed monthly by plating on PDA medium containing 150 ppm cycloheximide for C. terreus, and 150 ppm benomyl for C. albidus.  Yeasts population was expressed in log cfu/g materials.  To determine effect of carrier materials on antagonistic activity, bio-assay of formulated yeasts against pathogens was conducted after 3 months storage. Tested additives i.e. CaCl2, pure chitin and crab shell powder were added into suspension of C. albidus and C. terreus to get concentration of 1.25%,  0.5 %  and 0. 1% (w/v). Sterilized aquadest and yeasts without additives was used as control. Then  the treatments was examined the antagonistic activity through  bio-asaay  on detached banana fruits and tomato leaves. Appropriate additive(s) was determined by its ability to increase antagonistic activity of yeasts. Storability and antagonistic activity of  C. albidus dan C. terreus  in the mixture of best carrier and additive were examined.  C. terreus  was compatible to C. albidus. The best carrier materials supporting highest survival of C. terreus was tapioca and talc, those supported survival for four months storage.  Talc was best carrier material for C. albidus formulation by maintaining its survival for five months storage. Additives which able to increase antagonistic activity of C. terreus were CaCl2  0.1 % , pure chitin  0.1 %  and crab shell powder  0.5%.  Additives providing best increase of antagonistic activity of C. albidus were CaCl2 0.5%, pure chitin  1.25 %  and crab shell 1.25%.  Supplementation of chitin and crab shell, both at the rate of 1.25% into talc-base powder formulation  increased  survival of both yeasts at one months storage, however no significant effect subsequently.Key words: formulation, yeasts antagonists, Cryptococcus terreus, Cryptococcus albidus,  carrier agent, talc, tapioca, kaolin, CaCl2, chitin, crab shell powder
Introduction of the Serine Green Fluorescent Protein (sGFP) Gene into Pyricularia grisea Race dc4 Isolated from Digitaria ciliaris using Agrobacterium tumefaciens-mediated Genetic Transformation Stephanie, Stephanie; Widyastuti, Utut; Wiyono, Suryo
BIOTROPIA Vol. 22 No. 1 (2015): BIOTROPIA Vol. 22 No. 1 June 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2015.22.1.329

Abstract

Blast disease (caused by Pyricularia grisea) has long been known as a serious problem for upland rice, and recently, it has also begun infecting lowland rice. However, the mechanism enabling this range expansion is still unknown. One possible method to study this shift is by inserting a marker gene into P. grisea so that its spread can be monitored. The sGFP (Synthetic Green Fluorescent Protein) gene has been widely used as a reporter to track gene expression and cellular processes in fungi. In this study, the sGFP gene was integrated into the genome of P. grisea isolate DC4 from Digitaria ciliaris. The sGFP plasmid was first introduced into Agrobacterium tumefaciens using the triparental mating method (TPM). Genetic transformation was then carried out by co-cultivating spores of P. grisea DC4 with A. tumefaciens strain LBA4404 harboring the pCAMBIA-sGFP construct. Transformants were selected on medium containing 300 µg/mL hygromycin. Successful integration of the sGFP gene into the fungal genome was confirmed using PCR with sGFP-specific primers, an sGFP–Nos terminator primer pair, and a β-tubulin primer pair as an internal control. Fluorescent microscopy using blue light excitation confirmed sGFP expression in the P. grisea DC4 transformant.
Selection and Characterization of Siderophore-Producing Rhizobacteria and Potential Antagonistic Activity Toward Ralstonia solanacearum Nawangsih, Abdjad Asih; Parida, Ida; Wiyono, Suryo; Kartika, Juang Gema
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1186.787 KB) | DOI: 10.11598/btb.2017.24.2.406

Abstract

Ralstonia solanacearum is an important pathogen of tomato. An alternative method to control this disease is through the application of biocontrol agents. Plant Growth-Promoting Rhizobacteria (PGPR) can be used as potential biocontrol agents, and PGPR producing siderophores play an important role in disease suppression. This experiment was conducted to select and characterize siderophore-producing rhizobacteria from tomato and to determine their potential as antagonistic agents against R. solanacearum. Candidates of PGPR were isolated from tomato plants grown in West Java Province, Indonesia. The isolates were screened for siderophore production using CAS medium. Among the 29 siderophore-producing isolates that showed a negative hypersensitivity reaction, two isolates exhibited the widest inhibition zones against R. solanacearum. These isolates were CP1C and CP2D, with inhibition zone diameters of up to 3.6 mm and 7.0 mm, respectively. Based on 16S rDNA sequencing, isolate CP1C was identified as Brevundimonas sp., while isolate CP2D was identified as Enterobacter sp. Both isolates did not negatively affect plant height or plant dry weight when compared with the control.
Co-Authors . Haryanto . Rifka . Widodo . Widodo . Widodo Abdjad Asih Nawangsih Abdul Munif ABDUL MUNIF Adi Setyo Purnomo Agus Purwito Ahmad Fauzi Ridwan AHMAD JUNAEDI Ahmad Junaedy Ahmad Sutopo ALI NURMANSYAH Ali Wafa Aloysius Rusae Amrullah, Rizki Abi Anas Dinurrohman Susila Andika Septiana Suryaningsih Andriani, Desta Angelica Nabilla Ahdy Anisa Nurkasanah Anjar Kartika Antonius Suwanto ARI SUSILOWATI Arif Ravi Wibowo Aris Tri Wahyudi Asniah Asniah Astuti Kurnianingsih, Astuti Atit Kanti Atit Kanti Awang Maharijaya Ayu Rahayu Baskoro Sugeng Wibowo Bayu Aji Krisandi Bonjok Istiaji Burhanudin Burhanudin Cahyani, Gesa Nur DADANG DADANG DAMAYANTI BUCHORI Daniel Happy Putra Darni Rambu D. Siala Dedi Fardiaz Dewa Gede Wiryangga Selangga Dewa Gede Wiryangga Selangga DEWI SARTIAMI Dewi Sukma Dhany Nukhaizal Azkiya DWI ANDREAS SANTOSA Dwi Astuti DWI SUGIPRIHATINI Dyah Harianti Purnomo Efi Toding Tondok Eka Maulidiya, Sherly Eka Wijayanti Elis Nina Herliyana ENDANG MURNIATI Eryna Elfasari Rangkuti Evan P. Ramdan Fadillah, Arvin Muhammad Fakhrusy Zakariyya Fany Juliarti Panjaitan Fariyanti, Anna Fatah, Fadillah Abdul Fazad, M. Haikhal Febbiyanti, Tri Rapani Firman Hidayat Fitri Kemala Sandra Fitriansyah, Muhammad Ramdhani Giyanto Giyanto Giyanto Giyanto Giyanto Giyanto, Giyanto Halimah, Adedila Hanif, Andini HAPPY WIDIASTUTI Hari Priwiratama Harti, Heri Hendrastuti Hidayat, Sri Hendro Nurhadi Heny Setiyowati Heri Harti Hermanu Triwidodo Hidayah, Zahra Nurul Hidayat , Sri Hendrastuti I MADE ARTIKA I Made Sudiana I Made Sudiana I Nyoman Sumerta Idris Idris IKA DAMAYANTI Iman, Muhammad Ilham Nur Imaniasita, Vidya Indrapaksi, Tengku Ikhwan Alfajri Iskandar Z. Siregar Islah Hayati Ita Aprilia Ivone Oley Sumarauw Joni Hidayat, Joni Juang Gema Kartika Kartini Budiastuti Ketty Suketi Khairani Khairani Khairina, Winda Hidayatul Khamidi, Thamrin Khoirotul Afifah Kikin H Mutaqin Kristianto Nugroho Kristianto Nugroho Kudang Boro Seminar Kusuma Darma Kusuma Darma Kusumah, Yayi Munara Lestari, Mutiara Dwi Lilik Retnowati Lina Herlina Lina Herlina Listihani, Listihani M. Miftahudin Masrukhin Masrukhin Mei Lita Fitriani Meity S Sinaga Meity S. Sinaga Meity S. Sinaga MEITY S. SINAGA, MEITY S. Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Meity Suradji Sinaga Memen Surahman Mohamad Rahmad Suhartanto Mufidah, Iftin Farekana Muhammad Arief Munady Muhammad Lukman Hakim, Muhammad Lukman Muhammad Miftahudin Nanta, Wahyu Ridwan Napiudin Nazly Aswani Neo Endra Lelana nFN Khaerati Nika Ropiatningsuari Nur Alfi Saryanah Nur Khoiriyah Nurmala, Ayu Aulya Nurul Khumaida Octaviani, Wulan Nur Parida, Ida Phor Bho Ayuwati Pratama, Silvia Abdi Pratiwi, Astika Widhi Pratiwi, Tasya Resa Priyo Cahyono Puji Lestari Purnomo, Dyah Hariyanti Purwono Purwono, Purwono Puspitaningtyas, Khasanah Putri, Lystiana Dewi Putro, Herdayanto Sulistyo RAHAYU WIDYASTUTI Ramdan, Evan Purnama Reflinur Reflinur Reflinur Reflinur Remaja Sitepu Rerenstradika T. Terryana Riana Jumawati Rika Estria Gurusinga Rina Mardiana Rizki Abi Amrullah Roedhy Poerwanto Rohmah, Alya Awinatul Roza Yunita Rustam, Rustam Rustiani, Ummu S. Said Abdullah Saiful Akhyar Lubis Saputra, Bubun Afif Hidayat Aziz Sari Nurulita Sarwititi Sarwoprasodjo Satya Nugroho Sherly Eka Maulidiya Silviana Arsyad Sima Elpani Siregar, Amelia Nani Sitti Eha Faihah Sitti Fadhilah Slamet Susanto Sobir Sobir SRI FATMAWATI Sri Hartati Sri Hartati Sri Hartati SRI HENDRASTUTI HIDAYAT Sri Wening Stephanie Stephanie Sudarsono Sudirman Yahya Sudirman Yahya Sudirman Yahya Sudradjat Suhadi, Octen Sukmana, Andra Sahab Surya Rosa Putra, Surya Rosa Suryahadi Suryahadi Susanna Susanna SUSILOWATI1 SUSILOWATI1 Suwarno . Swastiko Priyambodo, Swastiko Tamrin Khamidi Tamrin Khamidi Tamrin Khamidi Tamrin Khamidi Teguh Iman Santoso Teguh Iman Santoso Titiek Siti Yuliani Toding Tondok, Efi Toga Pangihotan Napitupulu TRI ASMIRA DAMAYANTI Tri Rapani Febbiyanti Tri Rapani Febbiyanti, Tri Rapani Trikoesoemaningtyas Ummu S. Rustiani Ummu Salamah Rustiani Utami, Aditya Dyah UTUT WIDYASTUTI Vinsen Willi Wardhana Wahyu, Bonny Poernomo Wartono Wartono Wati, Cheppy Wawan Setiawan Weni Wilia Widhayanti, Nur Ari Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widodo Widya Sari Winarso D. Widodo Winda Ika Susanti Windah Meilanda Zulmi Yayu Siti Nurhasanah Yohana C Sulistyaningsih Yohana C Sulistyaningsih Yuli Fitriati Yuliawati YULIN LESTARI Yundari, Yundari Yuyun Andriyani