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Study of Pre and Post Supplementation Green Tea Extracts to MDA Levels Goenawan, Hanna; Lesmana, Ronny; Huda, Fathul; Baniasih, Akbar I.; Farenia, Reni; Purba, Ambrosius
Indonesian Journal of Clinical Pharmacy Vol 2, No 1 (2013)
Publisher : Universitas Padjadjaran

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Abstract

High intensity exercise (HI) induces oxidative stress. Running for 2400 meters is one of the simple exercise form which can be counted as HI. During HI, increase of oxygen consumption happened in muscle cell resulted in an increase of oxidant level. The aim of the study was to measure the effects of Indonesian green tea supplementation, before and after HI to the level of lipid peroxidation (TBARSMalonyldialdehyde (MDA)). The subjects of study were 42 male students from Faculty of Medicine, Universitas Padjadjaran (FMUP) and Faculty of Physical Exercise, Indonesia University of Education (FPOK-UPI). The subjects were divided into trained and untrained subject. Subjects were divided into groups using Astrand Harvard step test. The purpose of this test was measuring the level of VO2 max. Blood samples for MDA level were collected at 3 hours before and after high intensity aerobic  exercise. Oxidative stress was induced by 2400 meters run. The result showed that green tea supplement-ation was effective to decrease MDA plasma level especially after exercise. Green tea could serve as natural antioxidant resources, which potentially can be used as potential sport supplemental drink.Key words: Aerobic exercise, Malonyldialdehyde (MDA), pre and post exerciseStudi Pra dan Pasca Pemberian Suplemen Ekstrak Teh Hijauterhadap Kadar MDAHigh intensity exercise (HI) dapat menginduksi stres oksidatif. Berlari sejauh 2400 meter merupakan salah satu bentuk latihan sederhana yang dapat digolongkan sebagai HI. Selama HI, konsumsi oksigen pada sel otot meningkat yang dapat menyebabkan peningkatan kadar oksidan. Penelitian ini bertujuanuntuk mengukur efek dari suplemen teh hijau Indonesia sebelum dan setelah HI terhadap kadar lipid peroksida (TBARS-Malonyldialdehyde (MDA)). Subjek penelitian ini adalah 42 mahasiswa laki-laki dari Fakultas Kedokteran Universitas Padjadjaran dan Fakultas Pendidikan Olahraga dan Kesehatan,Universitas Pendidikan Indonesia. Subjek penelitian dibagi ke dalam kelompok terlatih dan tidak terlatih dengan menggunakan cara pengujian Astrand Harvard. Tes ini bertujuan untuk mengukur kadar VO2 max. Sampel darah untuk kadar MDA diambil 3 jam sebelum dan setelah high intensity aerobic exercise. Stres oksidatif diinduksi dengan berlari sejauh 2400 meter. Hasil penelitian menunjukkan bahwa suplemen teh hijau efektif dalam menurunkan kadar MDA dalam plasma khususnya setelah berlari. Teh hijau dapat menjadi sumber antioksidan alami yang berpotensi sebagai minuman suplemen olahraga.Kata kunci: Aerobic exercise, Malonyldialdehyde (MDA), sebelum dan setelah berlari
Early Detection of the Heavy Metals Pollution Effect on Citarum River Using Zebrafish Muscle Mitochondria Biomarkers Gene Expression Oktorina, Lenny; Goenawan, Hanna; Sylviana, Nova; Kirana, Kartika Hajar; Feriandi, Yudi; Lesmana, Ronny
Global Medical & Health Communication (GMHC) Vol 13, No 1 (2025)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v13i1.14110

Abstract

Citarum is the longest river in West Java and one of the most polluted rivers in Indonesia and the world. Heavy metals from agriculture, industry, and household waste pollute the Citarum river. Heavy metals enter humans and animals by consuming contaminated food and beverages. Several studies have identified mitochondria as a primary target for heavy metal poisoning, resulting in impaired mitochondrial energy production, induction of oxidative stress, apoptosis, and mitophagy. This study aims to investigate the impact of heavy metals from the upstream and downstream areas of the Citarum river on mitochondrial gene expression of cox4i1, sod2, baxa, mfn1b, and ppargc1a in the skeletal muscle of zebrafish (Danio rerio) using in vivo models for biological monitoring of early detection of environmental heavy metal pollution. This experimental study was conducted from December 2019 to March 2020. The study involved four treatment groups and one control group of zebrafish, which were exposed to river water and sediments collected from two sites in the Citarum river area in December 2019. The biomarker levels were analyzed using multivariate analysis. Although all heavy metal levels except mercury were below the WHO threshold in all samples, this study's results showed that the RT-PCR results indicated that the levels of cox4i1, baxa, and ppargc1a in all samples were generally higher than those in the control. There were significant differences (p<0.05) using multivariate analysis in sod2 in Cibeureum water compared to Cibeureum sediment and ppgarc1a compared to control and Balekambang sediment. In conclusion, heavy metals from the upstream and downstream areas of the Citarum river had an impact on mitochondrial gene expression of cox4i1, sod2, baxa, mfn1b, and ppargc1a in the skeletal muscle of zebrafish. Biomarkers pparc1gc and sod2 could be further studied to identify the most valid and reliable parameters for biological monitoring, which may aid in the early detection of environmental damage to humans and animals.
Correlation of Various Levels of Body Mass Index to Vascular Endothelial Growth Factor-A Permana, Noah Jefferson; Lesmana, Ronny; Setiawan, Setiawan; Lubis, Leonardo; Rosdianto, Aziiz Mardanarian; Rejeki, Purwo Sri
Althea Medical Journal Vol 12, No 3 (2025)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/amj.v12n3.4296

Abstract

Background: Obesity, reflected by high body mass index (BMI) value, has become a global health concern. Both obesity and vascular endothelial growth factor-A (VEGF-A) are major risk factors for cardiometabolic diseases. Although many studies have shown that obesity induces angiogenesis through increased VEGF-A, there remains a  gap regarding the correlation between BMI categories and VEGF-A levels. This study aimed to analyze the correlation between various level of BMI and VEGF-A concentrations.Methods: This cross-sectional in vivo study analyzed blood samples from 90 adults enrolled in the Universitas Padjadjaran Wellness Program ( December 2022–June 2023) selected using simple random sampling.VEGF-A concentrations were measured using multiplex ELISA assays. Data normality was assessed using the Kolmogorov-Smirnov test. Differences between BMI groups were analyze with ANOVA, and correlations were evaluated using Pearson’s test. Results: Of the 90 samples, 62 were included, and were grouped as normal weight (29%), overweight (32.2%), obesity class I (30.6%), obesity class II (6.4%), and obesity class III (1.6%). Mean VEGF-A concentrations showed an increasing trend with higher BMI, though differences between groups were not statistically significant (p=0.482). A weak positive correlation was observed between BMI and VEGF-A levels (r=0.267; p=0.036).Conclusion: Higher BMI is associated with higher VEGF-A, indicating obesity-induced inflammation and angiogenic activity. These findings highlight the importance of weight control through balanced diet and physical activity to mitigate long-term risks of cardiometabolic and chronic diseases.
Pengaruh Tablet Fe dan Kombinasi Tablet Fe dengan Yoga Pada Remaja Putri yang Mengalami Anemia dengan Dismenore Yunas, Aisya Astri; Syam, Hanom Husni; M.T., Vita; Anwar, Ruswana; Dhamayanti, Meita; Lesmana, Ronny
Jurnal Ners Vol. 9 No. 4 (2025): OKTOBER 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v9i4.49481

Abstract

Dysmenorrhea and anemia are common health problems among adolescent girls, while pharmacological therapy has limitations, highlighting the need for safe and comprehensive alternative interventions. This study aimed to analyze the effects of Fe supplementation and the combination of Fe supplementation with yoga on hemoglobin levels and dysmenorrhea intensity in adolescent girls. A quasi-experimental design with a two-group pre-test post-test approach was employed. Participants were adolescent girls from SMAN 03 Rejang Lebong who received either Fe supplementation or a combination of Fe supplementation and yoga for four weeks. Data were analyzed using the Wilcoxon and Mann-Whitney tests. The results showed that the combination of Fe supplementation and yoga significantly improved hemoglobin levels and reduced dysmenorrhea intensity (p = 0.000). Moreover, the combination was more effective than Fe supplementation alone. These findings suggest that combining yoga with Fe supplementation can serve as an effective non-pharmacological strategy to manage anemia and dysmenorrhea among adolescent girls. Keywords: dysmenorrhea, anemia, Fe supplementation, yoga, adolescent girls
Edukasi Pencegahan Stroke pada Masyarakat Desa Cigugur Girang: Stroke Prevention Education in Cigugur Girang Village Goenawan, Hanna; Tarawan, Vita Murniati; Radhiyanti, Putri Teesa; Ramadhanti, Julia; Lesmana, Ronny
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 8 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i8.7058

Abstract

Stroke patients could get sequelae, and it will decrease the patient’s quality of life. Stroke could be caused by comorbid diseases such as hypertension, diabetes mellitus, and dyslipidemia. Thus, stroke could be prevented by reducing the comorbid diseases. The prevention of stroke is necessary to be done in order to reduce stroke cases, especially in Bandung’s rural area. Villagers have limited access to health information and health services. In this community service, the methods we used were to screen the two risk factors of stroke (blood pressure and blood glucose ad random measurement) in Cigugur Girang Village, West Bandung area. The aim of this community service is to find comorbid factors of stroke and give education to people who have one or more stroke risk factors. The results of the blood pressure measurement screening showed that more than 40% of the residents of Cigugur Girang village had blood pressure above normal, and 5% of the population had blood sugar levels higher than normal. The risk factor for stroke in residents of Cigugur Girang Village mainly is high blood pressure. In this community service, people with high blood pressure were educated on how to maintain normal blood pressure and how to change their lifestyle. On the other hand, some participant has high blood glucose level. High blood glucose levels will develop into diabetes mellitus. In this education program, we gave information about the complications of high blood glucose, including stroke. Thus, this education program could increase community awareness to prevent stroke.
Hatha Yoga vs Prenatal gymnastic: The Perfect Choice to Reduce Stress and Improve Health for Primigravid Fernandez, Veronica; Lesmana, Ronny; Krisnadi, Sofie Riyani; Susiarno, Hadi; Tarawan, Vita Murniati; Iskandar, Shelly
JURNAL KEBIDANAN Vol. 15 No. 1 (2025): April 2025
Publisher : Politeknik Kesehatan Kementerian Kesehatan Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkb.v15i1.12677

Abstract

Abstract: Pregnancy is a critical period involving significant physiological and psychological changes, particularly in primigravida women who are more susceptible to anxiety and stress. Elevated stress levels during pregnancy may increase the risk of complications like high blood pressure, preeclampsia, and premature delivery. Non-pharmacological interventions like Hatha Yoga and gymnastic prenatalhave been widely recommended to improve maternal well-being. The purpose of this research was to evaluate and compare the impacts of two different interventions on maternal psychological and physiological health through a Systematic Literature Review (SLR) conducted in accordance with PRISMA guidelines. Relevant literature was retrieved from databases including Pubmed, Scopus, ScinceDirect, Google Scholar, focusing on research published between 2014 and 2024. The findings indicate that Hatha Yoga is more effective in reducing anxiety, cortisol levels, and systolic blood pressure, while prenatal gymnastic better supported diastolic blood pressure regulation and physical endurance for labor. Both interventions contribute to better cardiovascular function, increased oxygen saturation, and a smoother labor process. Additionally, yoga is associated with improved neonatal outcomes, while studies on prenatal exercise’s effects on newborn health remain limited. Future research should explore the long-term effects and key differences between these interventions to determine the most effective approach for maternal and neonatal health. These findings support integrating yoga and gymnastic prenatalinto antenatal care programs to enhance pregnancy outcomes.
The Comparative Effects of Hatha Yoga and Prenatal Exercise on Physiological and Psychological Outcomes in Primigravid Women: A Randomized Controlled Trial Fernandez, Veronica; Lesmana, Ronny; Krisnadi, Sofie R.; Susiarno, Hadi; Iskandar, Shelly; Tarawan, Vita Muniarti
Journal of Applied Nursing and Health Vol. 7 No. 3 (2025): Journal of Applied Nursing and Health
Publisher : Chakra Brahmanda Lentera Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55018/janh.v7i3.445

Abstract

Background: Primigravid women are prone to stress due to physical and psychological changes during pregnancy, which can lead to hypertension, preeclampsia, preterm birth, fetal growth restriction, and postpartum depression. While non-pharmacological interventions like hatha yoga and prenatal exercise are used to reduce stress, comparative evidence—especially on oxygen saturation (SpO₂)—is limited. This study analyzes the effects of hatha yoga and prenatal exercise on stress, blood pressure, and SpO₂ among primigravid women, and compares both interventions across these outcomes. Methods: This randomized controlled trial (RCT) followed the CONSORT 2010 guidelines. Forty-eight eligible primigravid women were selected using a probability sampling technique, specifically the simple random sampling method. Random numbers were generated using Microsoft Excel to determine participant selection from the maternal cohort register. Inclusion criteria were a normal singleton pregnancy at 24–28 weeks of gestation, maternal age 20–35 years, and normal Body Mass Index (BMI) (18.5–24.9 kg/m²). Exclusion criteria included regular exercise more than twice a week, abnormal fetal heart rate, and scheduled elective cesarean delivery. Participants were randomly assigned to either a hatha yoga group or a prenatal exercise group (24 each). Both interventions were conducted twice weekly for four weeks. Data were analyzed using the Wilcoxon and Mann–Whitney tests. Results: Data were analyzed using the Wilcoxon and Mann-Whitney tests. Median stress scores decreased from 22 to 12 in the hatha yoga group and from 22 to 17 in the prenatal exercise group (p < 0.001). Median SpO₂ increased from 96.88% to 98.38% and from 96.81% to 97.00%, respectively (p = 0.002). Median systolic pressure changed from 120.06 to 121.31 mmHg and 118.75 to 117.44 mmHg (p = 0.009), while diastolic pressure showed no significant difference (p = 0.076). Overall, hatha yoga showed greater improvements in stress, SpO₂, and blood pressure stability than prenatal exercise. Conclusion: Hatha yoga proved more effective than prenatal exercise in reducing stress, increasing SpO2, and stabilizing systolic blood pressure among primigravid women, although no significant difference was found in diastolic pressure. These findings strengthen the evidence supporting yoga as a safe, non-pharmacological approach to enhance maternal physiological and psychological well-being during pregnancy.
The Phenolic Compounds Isolated from Myristica fragrans and Their Cytotoxic Effects on B16-F10 Melanoma Cancer Cell Lines Hasbilla, Raihan Fathurrahman; Riyadi, Sandra Amalia; Safriansyah, Wahyu; Hidayat, Ace Tatang; Susianti, Susianti; Salam, Supriyanto; Lesmana, Ronny; Retnowati, Rurini; Supratman, Unang
Molekul Vol 20 No 3 (2025)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2025.20.3.16265

Abstract

Phenolic compounds are a major type of secondary metabolite found in plants. These compounds are synthesized through shikimic and phenylpropanoid pathways, resulting in the formation of numerous unique structures and bioactivities. In addition, a significant amount has been reported in nutmeg, an endemic plant of Indonesia, which has been widely used in traditional medicine. A previous study also revealed that ethyl acetate extract of the plant has notable cytotoxic effects against melanoma B16-F10. Therefore, the purpose of this study is to isolate and evaluate phenolic compounds in nutmeg for their potential to inhibit B16-F10 melanoma cancer cell growth. The seeds extract of nutmeg was separated by various chromatographic techniques to yield a total of five compounds, which were identified through spectroscopic analysis (HR-TOF-ESI-MS, IR, and NMR) as well as comparison with literature. The compounds 1-5 were identified as (+)-veraguensin (1), 3',4',5'-trimethoxycinnamyl alcohol (2), (+)-galbegin (3), (-)-polysphorin (4), and 7-methoxycoumarin (5). Cytotoxic effects were then assayed against B16-F10 melanoma cell lines using the Resazurin method. Furthermore, compound 1 displayed the highest cytotoxic activity, with an IC50 value of 112.71 µM.
Co-Authors . SETIAWAN . Susianti Abd. Rasyid Syamsuri Abdul Hadi Hassan Ace Tatang Hidayat Achadiyani Agus Muji Santoso Agus Syahrani Agus Syahrani Ahmad Kurniawan Ahmad Kurniawan Ahmad Ramdan Ajeng Diantini Aldo Hartono Aldo, Mochammad Alia Bihrajihant Raya Alia Bihrajihant Raya Ambrosius Purba Ambrosius Purba Anang Wahid M.Diah Anas Subarnas Anas Subarnas Andrew Citrawan Anggraeni Dyah S Anggraeni Dyah Sulistiowati Anissa Anissa Anissa, Anissa Annisa F. Dewi Arief Budiono Arief Cahyanto Arief S. Kartasasmita Arifin Dwi Saputro Arto Yuwono Soeroto Astrid Feinisa Khairani Astrid, Stephanie Aulina, Datin Zahira Aziis Mardanarian Rosdianto Aziiz Mardanarian Rosdianto Aziiz Mardanarian Rosdianto Aziiz Rosdianto Azis Mardanarian Azmi, Mohamad Nurul Bachtiar, Willy Bahjah, Firda Hanan Baniasih, Akbar I. Baniasih, Akbar I. Bashari, Muhammad H. Bela Ita Karina Daba, Mariel Dani Sujana Darmayanti, Meita Decky Gunawan, Decky Dewi, Annisa F. Diana K Jasaputra Diana Krisanti Jasaputra Diana Krisanti Jasaputra Diandra Syifa Dimpuulina Erna Mariati Dwi Putri Larasati Edi Sukmana Edi Sukmana Edward J Hadi Endang Sutedja Endang Sutedja Enny Rohmawaty Euis Julaeha Evi Rinata Fajar Ari Widyatmoko Fani Rahma Yenita Fathurachman Fathurachman Fatona Suraya, Fatona Febriana Kurniasari Ferdyan Efza Fernandez, Veronica Fifi Veronica Filza Yulina Ade Finda Meyditia Finda Meyditia Fitri Fadhilah Galuga Sinalusur Sari Genta Syaifrin Laudza Gilang Muhamad Nur Iqbal Goenadi, Julia Windi Gunadi, Julia Windi Gunawan, Hanna Hadi Hadi Hana Goenawan Hanna Goeanawan Hanna Goenawan Hanna Goenawan Hanna Goenawan Hanna Gunawan Hanom Husni Syam Hasbilla, Raihan Fathurrahman Hendra Gunawan Hendy Setiawan Hendy Setiawan Herlina, Lien Herry Herman Hilmi Al Wahid Huda, Fathul Huda, Fathul I Made Joni Ida Ayu Putu Sri Widnyani Ieva B. Akbar Ieva Baniasih Akbar Ika M. Silviana Imam Megantara Indahwati, Dwiwahju Dian Irma Erika Herawati Irma Erika Herawati Irma Melyani Puspitasari Irma Ruslina Defi Isa Mahendra Ivan Christian Channel Ivanna Valentina Iwan Setiawan Iwan Setiawan Iwan Setiawan Iwan Setiawan Jatnika, Danti Dja Jihan Mudrika Rahmi Julia W Gunadi Julia Windi Gunadi Julia Windi Gunadi Jutti Levita Kanakarajah, Sarmmila Kartika Hajar Kirana, Kartika Hajar Kastella, Faysal Keiichi Motoyama Khairinisa, Miski A. Krisnadi, Sofie Riyani Kusmawan, Steven Zerin Putra Lazuardhi Dwipa Leonardo Lubis M.T., Vita Madhani Pradiptha Nugroho Maharani, Julia Maimun Syukri, Maimun Mariel Daba Mas Rizky A.A. Syamsunarno MEGANTARA, SANDRA Meita Dhamayanti Muchtaridi Muchtaridi Mulyanaga, William Giovanni Mutakin Mutakin Mutiarahmi, Citra Nur Nabilah Muhamad Naini, Al Arofatus Nanang Wahyudin Nanang Wahyudin Nanny N. N. M. Soetedjo Nasrul Wathoni Norisca Aliza Putri Nova Sylviana Nova Sylviana Nuraini Yasmin Nuroh Najmi Nurul Fadhilah Oki Suwarsa Okta Wismandanu Oktorina, Lenny Pangestuti, Balqist Sriprobo Pardede, Akim Manaor Hara Penny Setyawati Martioso Permadi, Andieni Faqhira Permana, Noah Jefferson Permana, Shenny S.S Pinkan Al Shabrina Pitu Wulandari Prasetyo, Wibowo Budi Prayudi Santoso Putri Tessa Putri, Aufa A.D Qiantori, Adziqa Ammara Radhiyanti, Putri Teesa Rahdiyanti, Putri Teesa Ramadhanti, Julia Ramdani Hamzah Ray, Hamidie Ronald Daniel Rejeki, Purwo Sri Renaldi Prasetia Reni Farenia Reni Farenia rima siburian Riska A Safira Risna Agustina Risna Agustina Roro Wahyudianingsih Rosdianto, Aziis Mardanarian Rosdianto, Aziiz M. Rosdianto, Aziiz Mardanarian Rurini Retnowati Rusdin, Agus Ruswana Anwar Safriansyah, Wahyu Salam, Supriyanto Sandra Amalia Riyadi Santi Sofiyanti Sarmmila Kanakarajah Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Setiawan Shelly Iskandar Silviana, Ika M. Simanungkalit, Jayne Mary Siti Baitul Mukarromah Siti Mukarromah Siti Nuriyatus Zahrah Slamet Imron Sofie R. Krisnadi Sri Adi Sumiwi Steffi Liem Sudigdo Adi Sudrajat, Ratu Z.A.P sugianli, Adhi Sukmayani, Jasmine Y. Sunarjati Soedigdoadi Sunarjati Sudigdo Adi Sunarjati Sudigdo Adi Sunarjati Sudigdoadi Sunaryati S. Adi Supriatno Salam Susanti Husen Susianti Susianti Susianti Susianti Susianti, Susianti Susiarno, Hadi Tarawan, Vita M. Tarawan, Vita M. Teresa Lucretia, Teresa Teti Madiadipoera TIANA MILANDA Tina Rostinawati Titing Nurhayati Tjahjono D. Gondhowiardjo Tri Mayanti Trianing Tyas Kusuma Tyagita Hartady Unang Supratman Vita M. Tarawan Vita Muniarti Tarawan Vita Murniati Tarawan Vita Murniati Tarawan Vita Murniati Tarawan Lubis Vita Mutia Tarawan Wahyudianigsih, Roro Wahyuni, Indah S. Willy Bachtiar Yanuar Dwi Prastyo Yasmin, Nuraini Yenny Noor Yudi Feriandi Yulia Sofiatin Yumilia, Hoo Yunas, Aisya Astri Yuni Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi Yuni Susanti Pratiwi, Yuni Susanti Yusuf, Muchamad R.A Zaini, Lia M. Zakiyah, Neily Zulhendri, Felix