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Comparison Of Fixation Using NBF 10% and Honey 13% with Time Variation On Mice Heart Histology Preparations Using Hematoxylin Eosin Staining Amirah Putri Zerlina; Juliana Christyaningsih; Ratno Tri Utomo; Suliati Suliati
International Journal of Advanced Health Science and Technology Vol. 5 No. 4 (2025): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v5i4.515

Abstract

The fixation stage is the initial stage of making histopathology preparations whose role is to maintain the cell structure of the tissue so that it is like living tissue and prevent tissue damage, thus facilitating the process of macroscopic and microscopic analysis. The fixative solution that is often used for standard preservation in anatomical pathology laboratory examinations is Neutral Buffered Formalin (NBF) 10%, which has the advantage of a pH that is close to neutral and effective in fixing soft tissue for 12-24 hours. However, NBF 10% is carcinogenic and has high toxicity. There is a natural fixative solution, honey, which contains fructose and glucose and has an acidic pH that has antimicrobial, antioxidant and antiautolysis properties. In addition, honey has also been widely used as a preservative because it is easily available, affordable, and without toxicity effects. The purpose of this study was to determine the comparison of the quality of histology preparations of mice heart tissue fixed with 10% NBF and 13% honey with time variations. This research is a type of laboratory experimental research conducted in November 2024-April 2025. The sample used is the heart of healthy male mice. The number of sample preparations used was 27 sample preparations. Data analysis through scoring and statistically processed using non-parametric Kruskal-Walis test, if there is a difference followed by Mann-Whitney test. The results showed that fixation with 13% honey solution with a time variation of 24 hours has the same comparison of preparation quality results with 10% NBF solution for 24 hours. Based on the Mann-Whitney test, the result is 0.018 (p < 0.05) for 13% honey solution for 12 hours and 0.634 (p > 0.05) for 13% honey solution for 24 hours. It can be concluded that fixation with 13% honey solution for 24 hours is effective as an alternative fixation solution with natural ingredients that are safe in fixing tissues
Co-Authors ., Pestariati Adin Bhagawanti Poernomo Adistya Dwi Cantika Ahmad Yudianto Ainur Rifda Kurnia Puspita Sari Ainur Wachidah Choirun Niza Aisya Eliarosa Amiliya Antika Dewi Amirah Putri Zerlina Andriyani, Nurul Ani Intiyati Ani Media Harumi Ani Media Harumi Anita Dwi Anggraini Annas Buanasita Astuti Setiyani Astuti Setiyani Ayu Puspitasari Ayunis Naba'ur Rhodiyah Azfa Alifatun Nayla Bambang Hadi Sugito Briansky, Sabira Alvayogi Christ Kartika Rahayuningsih Christ Kartika Rahayuningsih Darjati Deddy Adam Dedi Kurniawan Demes Nurmayanti Deswita Aulia Pristanti Devi Eka Ratnasari Devi Puspitasari Diah Titik Mutiarawati Dian Shofiya Dwi Purwanti Dyah Purbosari Dwi Pitaloka Edy Haryanto Endarini, Lully Hani Endarini, Lully Hanni Endro Yulianto Ersanto, Novi Ervi Husni, Ervi Evi Yunita N Evi Yunita Nugrahini Evy Diah Woelansari Fahin Izza Faradyna Putri Az-Zahra Ferry Kriswandana Fika Nur Septianingtias Filisia Luna Andyana Firda Amalia Pratiwi Ginarsih, Yuni Hadi Suryono Halimah, Husnul Handayati, Anik HANIFA RAHMAWATI Haqqi, Faishol Harianto Notopuro Hermiyanti, Pratiwi Hilmi Yumni Hindaryani, Nurul Husnul Halimah I Dewa Gede Hari Wisana Ida Ariyanti, Ida Imam Sarwo Indah Lestari Indah Lestari Indah Lestari, Indah Inne Soesanti Ira Puspita Sari Ira Puspitasari Ira Puspitasari Ira Rahayu Tiyar Irwan Sulistio Isfentiani, Dina Islaha, Maria Isnaini, Dian Isnanto Istanto, Wisnu Jayeng Wijayanti, Erlyna Karina, Lidya Karina, Lidya Kasiati Khambali, Khambali Khristine Saputri Kiaonarn Ongko Waluyo Kiaonarni O.W Kiaonarni OW Kristiningsih, Elly Kuswanto, Muhammad Laili Rahmawati, Laili Lembunai Tat Alberta Lembunai Tat Alberta, Lembunai Tat Lieliek Soetjiatie Liliek Soetjiatie Liliek Soetjiatie Lully Hani Endarini Lully Hani Endarini Luthfi Rusyadi M. Zakarahman Makruf, Anang Mamik Mamik Mamik Mamik Marina Pengge Maulidyah Aisyah Putri Nadifah Meidayana, Ema Meisya Argia Sisviani Deva Melania Rizerda Pebianti Melina Sari MINARTI Minarti Minarti Minarti Minarti Mohd Nazil Salleh Mujayanto Mujayanto Muna Setia Madihah Museyaroh, Museyaroh Mutiarawati, Diah Titik N. S. Widodo Nadia Salsabila Nastiti, Aliffah Nurria Novita, Devy Nugroho, Riezky Faisal Nuning Nuning Marina Pengge Nur Hatijah Nur Hatijah Nur Hatijah Nur Kholifah, Siti Nurwening TW Nuswantari, Atika Oktavianingsih, Yulis P, Teresia Retna Patcharanee Pavadhgul Pebianti, Melania Rizerda Pengge, Nuning Marina Pojor Srirejeki Eka Lestari Purwanti, Dwi Puspita Zella Wigati Puspitadewi, Teresia Retna Puspitasari, Erlita Qonita Salsabilla Amara Sherly Amri Queen Khoirun Nisa' Mairo Rahayu Sumaningsih Rahayuningsih, Christ Kartika Ratna Sari Ratno Tri Utomo Regina Raiza Retno Puspitadewi, Teresia Retno Sasongko Wati Retno Sasongkowati Retno Sasongkowati Rihlah Sa'idah Rika Yulia Rokhmalia, Fitri Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Sari, Ira Rahayu Tiyar Sasongkowati, Retno sherly jeniawaty Sherly Jeniawaty Sholikah, Mar'atus Siagian, Hotmaida Siti Alfiah Siti Mar'atus Slamet Wardoyo Sri Sulami Endah Astuti SRI UTAMI Sri Utami Sugihani Suhariyadi Suhariyadi Suhariyadi Suhariyadi Suliati Suliati Suliati Suliati Suliati Suliati Suliati Sulistyowati, Dwi Wahyu Wulan Sumasto, Hery Supriyatin, Endah Suyanto, Beny Tatarini Ika Pipitcahyani Taufiqurrahman Taufiqurrahman Teta Puji Rahayu Tjipto Rini Toni Watoyani Trisnania, Iid Dani ulfa hidayah Umul Annisa Fadhila Utomo, Ratno Tri Vernanda Arsya Nabilla Veronica, . Veronica, . Wahyurianto, Yasin Win Darmanto Wisnu Istanto Wisnu Istanto Wulandari, Meisa Yohanes Kambaru W Yusianti Silviani Zida Shabrina Akmalia