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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Hortikultura Indonesia (JHI) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Zuriat BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Hortikultura Jurnal Penelitian Tanaman Industri Jurnal Tanaman Industri dan Penyegar Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Buletin Kebun Raya Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Planta Tropika Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropis ANNALES BOGORIENSES Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Martabe : Jurnal Pengabdian Kepada Masyarakat NUSANTARA : Jurnal Ilmu Pengetahuan Sosial JOURNAL OF SCIENCE AND SOCIAL RESEARCH CORD Jurnal Penelitian Tanaman Industri (Littri) Buletin Palma TEACHING : Jurnal Inovasi Keguruan dan Ilmu Pendidikan Tut Wuri Handayani : Jurnal Keguruan dan Ilmu Pendidikan Buitenzorg: Journal of Tropical Science Jurnal Natur Indonesia Annales Bogorienses
Dewi Sukma
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor. Jl. Meranti, Kampus IPB Dramaga, Bogor, 16680, Indonesia
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Studi In Silico Sekuen NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) pada Jeruk yang Terkait dengan Resistensi Huanglongbing Nugroho, Kristianto; Santoso, Tri Joko; Kosmiatin, Mia; Sukma, Dewi; Purwito, Agus
Buitenzorg: Journal of Tropical Science Vol 2 No 2 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i2.25

Abstract

The evaluation of citrus resistance to Huanglongbing (HLB) disease is still challenging due to the incapability of the bacteria to be cultured purely in artificial medium, the complexity of inoculation methods, and the long duration required for phenotypic observation. Thus, the use of molecular markers is one of the alternatives to solve this problem. The focuses of this study were to perform in silico analysis of the nucleotide variations in NONEXPRESSOR OF PATHOGENESIS-RELATED GENES 1 (NPR1) gene sequence among several citrus genotypes whose resistance information to HLB have been known previously and to employ phylogenetic analysis among them. The NPR1 gene sequences from 20 genotypes which consisted of 14 citrus and six relative genotypes were collected in silico from Citrus Genome Database and analyzed using multiple sequence alignment program. A total of six interesting SNPs that could distinguish between susceptible and resistant citrus genotypes were detected in this study. As many as five SNPs were non-synonymous, while only one synonymous SNP that did not cause the amino acid change was identified in this study. The phylogenetic analysis also revealed the separation between susceptible and tolerant/resistant citrus genotypes in two main clusters. The SNPs found in this study are expected to be useful for designing new functional markers as a selection tool in future studies. Keywords: in silico, molecular marker, phylogenetic analysis, selection tool, SNP
Pengaruh Silika dan Media Tanam terhadap Keberhasilan Aklimatisasi dan Pertumbuhan Planlet Dendrobium violaceoflavens x Dendrobium canaliculatum Nurgravisi, Primanisa; Sukma, Dewi; Iis Aisyah, Syarifah
Buletin Agrohorti Vol. 13 No. 3 (2025): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.13.3.70605

Abstract

Anggrek Dendrobium section Spatulata hasil hibridisasi yang baru dikembangkan di Indonesia adalah Dendrobium violaceoflavens x Dendrobium canaliculatum. Perbanyakan melalui kultur jaringan dapat menjadi salah satu alternatif perbanyakannya, namun metode ini memiliki fase aklimatisasi yang sangat rentan dan sering kali menyebabkan tingkat kematian di atas 50% jika kondisi lingkungan tidak optimal. Penelitian ini bertujuan mengetahui pengaruh silika dan media tanam pada pertumbuhan Dendrobium violaceoflavens x Dendrobium canaliculatum pada masa aklimatisasi.  Unsur silika dipilih karena dapat meningkatkan ketahanan terhadap cekaman pada tanaman. Rancangan yang digunakan adalah Rancangan Petak Terbagi (RPT) dua faktor. Faktor pertama adalah konsentrasi silika (SiO2) dengan empat taraf konsentrasi; 0 g L-1, 0.01 g L-1, 0.02 g L-1 dan 0.03 g L-1. Faktor kedua yaitu perlakuan media tanam sebanyak tiga jenis; spaghnum moss, moss hitam dan sabut kelapa. Hasil penelitian menunjukkan perlakuan konsentrasi silika tidak memberikan pengaruh nyata terhadap jumlah daun, tinggi tanaman, kerapatan stomata, ketebalan epidermis dan persentase hidup. Sementara itu, perlakuan berbagai media tanam berpengaruh nyata pada jumlah daun, tinggi tanaman, kerapatan stomata, ketebalan epidermis dan persentase hidup. Media tanam terbaik adalah moss hitam dan sabut kelapa. Interaksi perlakuan konsentrasi silika dan media tanam pada peningkatan ketebalan epidermis dan berpengaruh nyata pada kerapatan stomata. Kata kunci: anggrek, epidermis, silika, stomata
Co-Authors , Krisantini , Nazi , Rustikawati ,, Giyanto ABDUL MUNIF Abdullah bin Arif Adil Akbar Muhammad Adimas Krishardianto Affif Andra Saputro Agus Purwito Agus Rachmat Agus Rachmat Ahmad Bukhori, Ahmad Ahmad Fathoni Aji, Titistyas Gusti Akbar, Machmul Alam Sani, Fajri Ali Husni Alvianti Yaufa Desita Alwi, Ahmad Andi Zahira Al Humairoh Andri, Eldrida Antony Demas Apriliani, Elisa Aqilla Fadia Wahono Arifah Rahayu Arrin Rosmala Ary Setiawati Assyifa Fauzia Atra Romeida Avisena, Dimas Lutfi Azmi, Tubagus Kiki Kawakibi Budi Marwoto Chaireni Martasari Cici Tresniawati Daffa Rafiandra Haryono Daulay, Delfina Deden Derajat Matra Deden Sukmajaya Demas, Antony Dewa Ngurah Suprapta DINI NURDIANI Dini Nurdiani Dirga, Kate Tiffany Dodi Saputra, Muhammad Dwi Utami Nur Usmani Efendi, Darda Eka Martha Della Rahayu Elina, Juanita Elis Nina Herliyana Elizabeth Handini ELSJE T. TENDA ELSJE T. TENDA, ELSJE T. ENNY RANDRIANI Erick Raynalta Erick Raynalta, Erick Erna Sulistiana Erna Sulistiana Fadillah, Anggun Fajar Pangestu Fauziah, Ni'mah Fawaz, Ataya Thalita Fuady Dharma Harahap, Andes Giyanto, dan Gustaaf Adolf Wattimena HAJRIAL ASWIDINNOOR Halimatussakdiyah, Halimatussakdiyah Handini, Aline Sisi Hasibuan, Nadia HENGKY NOVARIANTO Hengky Novarianto Hengky Novarianto Hermanto, Catur Hidayatullah, Deden Husein Nst, Ahmad I MADE ARTIKA Ika Roostika Indira Ariesta Ira Fauziah Noer Irawati Irawati Islami, Muhammad Randy ISMAIL MASKROMO Ismail Maskromo Ismail Maskromo Ismiati, Mely Isnaini Harahap, Fitri Iswari S. Dewi Juang Gema Kartika Kartiman, Roni Kartiman, Roni Khoiruddin Khoiruddin Khoiuruddin, Khoiruddin Krishardianto, Adimas KRISTIANTO NUGROHO, KRISTIANTO Kultsum, Fifit Lestari Koto, Putri Lolliani Lubis, Uchti Nuzul Qhinanti M. Syukur Maria Swastika Maryuandini, Ufi MASKROMO, ISMAIL MASKROMO, ISMAIL Mastur Mastur Megawati, Gina MEITY A. TULALO MEITY A. TULALO, MEITY A. Mia Kosmiatin Mira Humaira Muhamad Rizal Martua Damanik Muhamad Syukur Muhammad Hamzah Aditama Muhammad Syukur Muhammad Syukur Muthi'ah Khairun Nisa Muthi’ah Khairun Nisa Nabilah, Noorfakhriyah Ahsanti Nasution, Fery Endang Ni'mah Fauziah Noer, Ira Fauziah NOVARIANTO, HENGKY NOVARIANTO, HENGKY Nur Ajijah NUR AJIJAH Nurgravisi, Primanisa Nurul Khumaida OKTA ROSFIANI Pebra Heriansyah Pratama, Syilvia Nosya PUJI LESTARI Pulungan, Darmasyah Pulungan, Nurpadilah Putri, Astrid Aditya Putri, Halida Adistya Putri, Yane Riana Rahmawati, Rika Sri rawati ,, rawati Razaq, Sahid Muhammad Refa Firgiyanto REFLINUR REFLINUR Risda Rizkillah, Risda Riski Ananda, Ihwan Rizal, Syamsi Roedhy Poerwanto RUBIYO RUBIYO Rusmiyati, Henny S. Sudarsono Sachio, Sachio Safri Hasibuan, Iskandar Saidah, Marwatu Sandi, Gunawan Sandra A. Aziz Sandra Arifin Aziz Saputra, Galang Rafly Yus Sari, Novila Satya Nugroho Satya Nugroho Sauri Nasution, Sofyan Shandra Amarilis Shandra Amarillis Siberakuno, Roberdi Sihombing, Novi Silviyani Siregar, Sindi Simatupang, Juli Simatupang, Juliana Sintho Wahyuning Ardie Siti Salamah Slamet Susanto Sormin, Darlina Sudarsono Sudarsono SUDARSONO SUDARSONO SUDARSONO SUDARSONO Sudarsono Sudarsono Sudarsono, dan SUDARSONO, nFn SUDARSONO, nFn Sudarsono, Sudarsono Sugianto, Pingkan Sukendah SUKENDAH SUKENDAH SUKENDAH SUKENDAH, SUKENDAH SUKENDAH, nFn SUKENDAH, nFn Sukmana, Bayu Surjono Hadi Sutjahjo Suryo Wiyono Suskandari Kartikaningrum Sutanto, Agus Swastika, Maria Swastika, Maria Syafa, Naila Syarifah Iis Aisyah Syilvia Nosya Pratama Tony Liwang TRI JOKO SANTOSO Tya Wulandari Ucu Sumirat Wahyu Arif Sudarsono Wahyu Arif Sudarsono Waras Nurcholis Willy Bayuardi Suwarno Yafqori Ardigusa Yasmin, Zahra Fadhlia Yoshua Shandy Yudha Yudhi, Joshinjitsu Fatirizqina Yustia Yulianti Zahra Fadhlia Yasmin Zulfa, Aqila