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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner The Journal of Experimental Life Sciences (JELS) Journal of Indonesian Tourism and Development Studies Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Biotropika Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari Jurnal Ternak Tropika Journal of Development Research Animal Production : Indonesian Journal of Animal Production Jurnal Penelitian Pendidikan IPA (JPPIPA) JURNAL ILMIAH PETERNAKAN TERPADU AGROMIX Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Peternakan Nusantara Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Budapest International Research in Exact Sciences (BirEx Journal) Jurnal Pengabdian Masyarakat Asia Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Eduvest - Journal of Universal Studies Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Journal of Livestock and Animal Health (JLAH) Journal of Fisheries & Marine Animal Production Indonesian Journal of Forestry Research
Gatot Ciptadi
Department Of Livestock Production, Faculty Of Animal Husbandry, Universitas Brawijaya
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Journal : Research Journal of Life Science

 1 
Post Thawing Sperm Quality and Ca+2 Intensity Characters of Local Goat Sperm After Freezing by Simple Method Using Deep Freezing Gatot Ciptadi; Muhammad Nur Ihsan; Sri Rahayu; Sri Wahjuningsih; Anis Mei Munazaroh; R. P. Putra
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (775.085 KB) | DOI: 10.21776/ub.rjls.2017.004.03.3

Abstract

The objective of this research was to determine the effect of the simple modified freezing method, 1°C/minute freezing rate with different diluter ration on a post-thawing quality of local goat sperm namely Peranakan Etawah (PE). This work is aimed to study the quality of post-thawing sperm and to characterize the calcium intensity profile of both fresh and post thawing goat sperm. The method used is the experimental design of a laboratory. Freezing semen was performed in 2 main temperatures of -45°C then -196°C respectively using Mr. Frosty (®) System. Early Sperm characters of Ca+2 intensity was performed by Confocal Laser Scanning Microscope (CLSM) through Fluo-3 staining and Ca++ intensity was analysis descriptively. The result showed that post-thawing qualities are considered as good as standard qualities, at least, more than 40% based on Indonesian National Standard (SNI, 2014). The different level diluents commercial of Andromeda used were influenced highly significant (P<0.01). The best diluents ration is 1:4 (v/v) for final sperms stocked at -196°C. However freezing sperm conserved in -196°C is better than in -45°C. Meanwhile, the sperm characters of two condition showed the important variation of Ca+2 intensity, with the length of region measurement of 39.06±4.595 and 32.696±9.011 µm each.  It was concluded that the calcium intensity pattern was varied more and higher in fresh sperm than in freezing sperms. This simple modified method of a freezing system was considered as a feasible alternative method for goat semen in a reason for both for sperm post-thawing quality and practical purposes.
The Comparison of Chromosome Analysis Result by Manual and Software Cytovision Image Analysis Using Simple G-Banding Gatot Ciptadi; Muhammad Nur Ihsan; Sri Rahayu; Veronica Margareta Ani Nurgiartiningsih; Mudawamah Mudawamah; Ardyah Ramadhina Irsanti Putri
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.513 KB) | DOI: 10.21776/ub.rjls.2017.004.02.3

Abstract

Chromosome analysis or karyotyping is one among powerful methods to characterize normal or abnormal genetic of animals. On the basis of the important chromosome abnormalities and their negative effect in the near future, chromosomal investigation of breeding bull especially for Artificial Insemination (AI) began in different countries. Chromosomal abnormalities are usually considered to be a plague and are to eliminate. In Indonesia, AI implementation in cattle have been started intensively, especially using imported bulls (bos Taurus) i.e. Limousine. A number of cattle breeds have been reported on of the 50 exotic breeds with the problem of 1/29 translocation, then the chromosome analysis to be important to execute. Method performed by collecting blood samples from first generation of crossing breed Madura Cattle vs. Bos Taurus. Sample was added to medium (Karyo MAX Gibco) then placed in CO2 incubator at 38°C. Colcichine was added after 70 hours and kept for 2–3 hours. Slides were prepared and dried then stained with Giemsa. Slides were examined under high phase-contrast microscope, chromosome analysis using cytovision software and manual analysis straightly captured under microscope then arranged. Result of both method of karyotyping may accepted for analysing method of abnormal/normal chromosome. It showed that the 2 N diploid number of chromosome was normal was 60, there were 58 autosomes and 2 sex chromosomes in all cattle observed. It was observed that all cattle tested in this research were normal categories.  The karyotype analysis of all cattle showed that the chromosomes of one cell and different individual each breed varied in size, shape and position of centromere. It was recommended to performed chromosomal investigation of breeding bulls using advanced sophisticated tools of analysis like cytovision image analysis of fluorescent technique. Manual method was recommended only for analysis of normal or abnormal number of chromosome.
Co-Authors A, Aulanniam Abdul Hakim Abdul Rahem Faqih Achmad Iklilul L.L. As’ad Adelina Ari Hamiyanti Adelina Ari Hamiyanti Adriani Sunuddin Agung Pramana W.M Agung Pramana W.M Agung Pramana Warih Marhendra Agus Budiarto Agus Budiarto Agus Budiarto Agus Budiarto Agus Suhadi Agus Susanto Akhiroh, Puji Ali Harris Andik Isdianto Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Apriani Herni Rophi Ardyah Ramadhina Irsanti Putri Ardyah Ramadhina Irsanti Putri Ardyah Ramadhina Irsanti Putri Aris Subagyo Asma Naili Salsabila Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Aulia Puspita Anugra Yekti Azis, Rosidi Badriyah B Badriyah B, Badriyah Badrus Sholeh Bambang Semedi Bambang Susilo Bilqis Bilqis Brehaspati, Eka Wara Budi Siswanto Budi Siswanto Darmawan, Drajad Azis Dewi, Citra Satrya Utama Dian Ratnawati Diani, Khonita Rahma Doni Herviyanto Dyah Kinasih Wuragil Putu Raharjo Eko Wahyudi Enni Mutiati Eva Ari Wahyuni Eva Ari Wahyuni Fadillah Putra Fahmi, Mohammad Bachtiar Fitra, Ardian Gladys Agnescia Sumampouw Hagus Tarno Harianto, Eirene Harsuko Riniwati Hemiyanti, Adelina Ari Herandarudewi, Sekar Mira I.D. Retnaningtyas I.D. Retnaningtyas, I.D. Insafitri, I Irawati Dinasari Retnaningtyas Juwita Ratna Dewi Karim Khalifa Zamuna Khonita R. Diani Khonita Rahma Diani KIKI FIBRIANTO Kuswati Kuswati Laili Windah Fauziah Lilik Eka Radiati Liza Choirun Nisa M, Nur Ihsan M. Ruslin Anwar M.F. Wadjdi M.F. Wadjdi, M.F. Maharani Pertiwi K. Marjono Marjono Marjono Marjuki Marjuki Meyya L. Zain Mirsa Ita Dewi Moch. Aris Sunaryo Moch. Nasich Mohammad Bisri Mohammad Nur Ihsan Mubarak Z, Sultan Mudawamah Mudawamah Mudawamah Mudawamah Mudawamah Mudawamah Muhammad Arwani Muhammad Musa Muhammad Nur Ihsan Muhammad Nur Ihsan Muhammad Rizar Zakaria Muhammad Sumsanto Nail, Rory Abduh Nasirudin, Mohamad Nico Rahman Caesar Nuhfil Hanani Nur Ihsan Nur Ihsan Nurgiartiningsih, V. M. A. Nurhabib, Asro Nurul Jadid Mubarokati Nuryadi Nuryadi Putranto, Septiawan Putri, Ardyah Irsanti R. R. P. Putra Retnaningtyas, irawati Dinasari Ria Dewi Andriani Rizka Gitta Almaida Rizki Putra Samudra Roy Ardy Colas Napitupulu S. Chuzaemi S. Susilowati S. Susilowati Sambou, Omar Samudra, Rizki Putra Samwil, Samwil Septi Anitasari, Septi Septian Ragil Anandita Septiawan Putranto Setyawan, Hendrix Yulis Simbolon, Rosdiana Siska Nanda Widhaningrum Slamet Wahyudi Sony Susanto Sri Hindrawati Sri Rahayu sri rahayu Sri Rahayu Sri Rahayu Sri Rahayu SRI RAHAYU Sri Rahayu Sri Rahayu Sri Wahjuningsih Sucik Maylinda Sukandar Sukandar Sulistyoningtyas I. Sunaryo Sunaryo Sunaryo, Moch. Aris Sutiman B. Sumitro Sutiman Bambang Sumitro Sutopo, Bima Prakasa Dermawan Suyadi Suyadi Suyadi Suyadi Syafrizal Muhammad Syahrul Kurniawan Syamsul Arifin Teguh Wicaksono Tri Eko Susilorini Trinil Susilawati Trinil Susilawati V.M. Ani Nurgiartiningsih Wardani, Nezya Pramudya Wiadnya , Dewa Gede Raka Wike, Wike Wiwik Handayani Woro Busono, Woro Wresti Listu Anggayasti Wulandari, Eka Shinta Yanuhar, Uun Yudit Oktanella, Yudit Zulvado Satria Putra Yuwono