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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Veteriner The Journal of Experimental Life Sciences (JELS) Journal of Indonesian Tourism and Development Studies Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Biotropika Jurnal Sain Peternakan Indonesia Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Journal of Innovation and Applied Technology Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari Jurnal Ternak Tropika Journal of Development Research Animal Production : Indonesian Journal of Animal Production Jurnal Penelitian Pendidikan IPA (JPPIPA) JURNAL ILMIAH PETERNAKAN TERPADU AGROMIX Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Sciences) Jurnal Peternakan Nusantara Abdimas Umtas : Jurnal Pengabdian kepada Masyarakat Budapest International Research in Exact Sciences (BirEx Journal) Jurnal Pengabdian Masyarakat Asia Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Eduvest - Journal of Universal Studies Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Journal of Livestock and Animal Health (JLAH) Journal of Fisheries & Marine Animal Production Indonesian Journal of Forestry Research
Gatot Ciptadi
Department Of Livestock Production, Faculty Of Animal Husbandry, Universitas Brawijaya
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Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Mubarakati, Nurul Jadid; Aulanni'am, Aulanni'am; Sumitro, Sutiman Bambang; Ciptadi, Gatot
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
Bovine And Human Zona Pellucida 3 Gene Glycans Site Prediction Using In Silico Analysis Nurul Jadid Mubarakati; Aulanni'am Aulanni'am; Sutiman Bambang Sumitro; Gatot Ciptadi
Journal of Tropical Life Science Vol. 4 No. 3 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.04.03.08

Abstract

Zona pellucida is one of the protective layer of the egg cell and has a function as an intermediary species-specific fertilization. Glycoproteins of human and bovine zona pellucida is composed of three types, namely ZP1, ZP2 and ZP3. ZP3 gene has amino acid sequence homology with other mammals. Oligosaccharides components of the zona pellucida glycans are composed from units of asparagine residues (N -linked) and serine/threonine (O -linked). The aims of this study was to analyze the DNA sequences of human and bovine and further predicts glycans site on amino acid sequence of human and bovine ZP3. In this study, ZP3 gene fragments have been isolated from bovine and humans were analyzed in Silico. This work were conducted by comparing the data of DNA sequence from human and bovine PCR product using NCBI BLAST. The results showed that there were similarities at amino acid positions number 23-38. bZP3 sequence had three glycans site (Asn-X-Thr/Ser) and one site on hZP3 glycans. One of the sites was conserved between the two species.
Co-Authors A, Aulanniam Abdul Hakim Abdul Rahem Faqih Achmad Iklilul L.L. As’ad Adelina Ari Hamiyanti Adelina Ari Hamiyanti Adriani Sunuddin Agung Pramana W.M Agung Pramana W.M Agus Budiarto Agus Budiarto Agus Budiarto Agus Budiarto Agus Susanto Akhiroh, Puji Ali Harris Andik Isdianto Angelina N. Tethool Anis Mei Munazaroh Anis Mei Munazaroh Apriani Herni Rophi Ardyah Ramadhina Irsanti Putri Ardyah Ramadhina Irsanti Putri Aris Subagyo Asma Naili Salsabila Aulanni'am Aulanni'am Aulanni'am, Aulanni'am Aulia Puspita Anugra Yekti Azis, Rosidi Badriyah B Badriyah B, Badriyah Badrus Sholeh Bambang Semedi Bambang Susilo Bilqis Bilqis Brehaspati, Eka Wara Budi Siswanto Budi Siswanto Darmawan, Drajad Azis Dewi, Citra Satrya Utama Dian Ratnawati Diani, Khonita Rahma Doni Herviyanto Dyah Kinasih Wuragil Putu Raharjo Eko Wahyudi Enni Mutiati Eva Ari Wahyuni Eva Ari Wahyuni Fadillah Putra Fahmi, Mohammad Bachtiar Fitra, Ardian Hagus Tarno Harsuko Riniwati Hemiyanti, Adelina Ari Herandarudewi, Sekar Mira I.D. Retnaningtyas I.D. Retnaningtyas, I.D. Insafitri, I Irawati Dinasari Retnaningtyas Karim Khalifa Zamuna Khonita R. Diani Khonita Rahma Diani KIKI FIBRIANTO Kuswati Kuswati Laili Windah Fauziah Lilik Eka Radiati Liza Choirun Nisa M, Nur Ihsan M. Ruslin Anwar M.F. Wadjdi M.F. Wadjdi, M.F. Marjono Marjono Marjuki Marjuki Meyya L. Zain Mirsa Ita Dewi Moch. Aris Sunaryo Moch. Nasich Mohammad Bisri Mohammad Nur Ihsan Mubarak Z, Sultan Mudawamah Mudawamah Mudawamah Mudawamah Mudawamah Mudawamah Muhammad Arwani Muhammad Musa Muhammad Nur Ihsan Muhammad Nur Ihsan Muhammad Rizar Zakaria Muhammad Sumsanto Nail, Rory Abduh Nico Rahman Caesar Nuhfil Hanani Nur Ihsan Nur Ihsan Nurgiartiningsih, V. M. A. Nurhabib, Asro Nurul Jadid Mubarokati Nuryadi Nuryadi Putranto, Septiawan Putri, Ardyah Irsanti R. R. P. Putra Retnaningtyas, irawati Dinasari Ria Dewi Andriani Rizka Gitta Almaida Rizki Putra Samudra Roy Ardy Colas Napitupulu S. Chuzaemi S. Susilowati S. Susilowati Sambou, Omar Samudra, Rizki Putra Septi Anitasari, Septi Septiawan Putranto Setyawan, Hendrix Yulis Simbolon, Rosdiana Siska Nanda Widhaningrum Slamet Wahyudi Sony Susanto Sri Hindrawati Sri Rahayu Sri Rahayu SRI RAHAYU Sri Rahayu Sri Rahayu Sri Rahayu sri rahayu Sri Rahayu Sri Wahjuningsih Sucik Maylinda Sukandar Sukandar Sulistyoningtyas I. Sunaryo Sunaryo Sunaryo, Moch. Aris Sutiman B. Sumitro Sutiman Bambang Sumitro Sutopo, Bima Prakasa Dermawan Suyadi Suyadi Suyadi Suyadi Syafrizal Muhammad Syahrul Kurniawan Syamsul Arifin Teguh Wicaksono Tri Eko Susilorini Trinil Susilawati Trinil Susilawati V.M. Ani Nurgiartiningsih Wardani, Nezya Pramudya Wiadnya , Dewa Gede Raka Wike, Wike Wiwik Handayani Woro Busono, Woro Wresti Listu Anggayasti Wulandari, Eka Shinta Yanuhar, Uun Yudit Oktanella, Yudit Zulvado Satria Putra Yuwono