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Journal : Journal of Global Pharma Technology

Determination of lactic acid bacteria isolated from the gastrointestinal tract of Bali cattle with potential probiotic role Dewa Made Sukrama; I Wayan Suardana
Journal of Global Pharma Technology Volume 10 Issue 08: (2018) August 2018
Publisher : Journal of Global Pharma Technology

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Abstract

Bali cattle is known as pioneer’ cattle resulted by their high ability to adapt in poor quality of feed so that was assumed for finding the specific species of lactic acid bacteria.  The study aimed to investigate the potency of lactic acid bacteria isolated from the gastrointestinal tract of Bali’ cattle as a candidate for new probiotic. Fifteen isolates of lactic acid bacteria isolated from 15 intestinal tracts of Bali cattle were grown on the specific medium deMan Rogosa Sharpe (MRS) broth followed by Gram staining, catalase test, the formation of CO2 gas, and antimicrobial activities against Bacillus cereus. The result of this study confirmed 15 isolates were Gram-positive bacteria, and 14 out of 15 isolates were categorized as homofermentative. Probiotic potency test showed 11 out of 15 isolates were tolerant against lower pH (pH 2, 4 and 6), and also resistant to bile acid with NaDC concentration 0.2, 0.4, and 0.6 mM.  Identification of isolate using API 50CH test kit found 4 out of 5 isolates as Lactobacillus sp. and the remainder as Lactococcus sp. The study concluded isolates of lactic acid bacteria isolated from the gastrointestinal tract of Bali cattle showed potential as a probiotic candidate.
MOLECULAR ANALYSIS OF LACTIC ACID BACTERIA ISOLATE SR2 FROM BALI CATTLE RUMEN I Dewa Made Sukrama; Komang Januartha Putra Pinatih; I Wayan Suardana
Journal of Global Pharma Technology Volume 09 Issue 05
Publisher : Journal of Global Pharma Technology

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Abstract

Bali cattle with its distinctively genetic characteristics, namely simple life or easy to adapt on unfavorable environmental condition was known as cattle pioneer. These characteristics were due to specific type of lactic acid bacteria (LAB). These facts indicate that the LAB identification with specific advantages was absolutely necessary for further research in order to prevent incorrect interpretation. Generally, molecular approach is the accurate method of identifying microorganism, and in this case, we used the analysis of the 16S rRNA gene. Research activities began with cultivation of SR2 isolates on specific media deMann Rogosa Sharpe (MRS) followed by Gram stain, catalase test, and formation of CO2 gas. Molecular analysis was performed by PCR of the 16S rRNA gene using primers 27F and 1492R. The PCR products were sequenced and nucleotides data were analyzed by Mega 5.2 Software tool. The results showed that phenotypical isolates of SR2 showed characteristics as LAB isolates, and the results of the 16S rRNA gene analysis showed that isolates SR2 included into Lactococcus lactis strain with a bootstrap value of 100.Keywords - Bali cattle, lactic acid bacteria, molecular analysis, rumen, SR2 isolate
Co-Authors Abdul Manan Achjar, Komang Ayu Henny Adirinata, I Komang Pasek Agus Sri Lestari Amrulloh, Muhammad Faqih Anak Agung Gde Bagus Udayana Anderson Ngelambong Andi Isma Lestari Amin, Andi Isma Anom Hery Suasapha Ardika, I Gusti Ngurah Putu Ari Yuniastuti Aribaten, Ni Nengah Zinnia Arimbawa, I Gede Artayani, Ida Ayu Gede Asyauqi Ilham Perdana, Asyauqi Ilham Aungsuroch, Yupin Bambang Sumiarto Bangun Mulia, Victor Bq Nurlita Anugrah BUDI SETIADI DARYONO Budi Setiadi Dayono Chandra Yowani D. A. Widiasih Denny Widaya Lukman Devi Dwi Jayanti Dewa Gede Agung Widyadnyana Dewa Putu Oka Prasiasa Dewi, Ni Nyoman Astika Dewi, Ni Putu Diah Trisna Dewintasari, Ni Nyoman Paramitha Dwi Lestari Dyah Ayu Widiasih Dyah Ayu Widiasih Dyah Ayu Widiasih Eka Putri Suryantari Emmanuella Felice’anna Dije Karisoh Febri Diana Putri, Ni Putu Febrianti, Andri Nurdiana Franciska, Juliana Gama, I Ketut Gama, Ketut Gede Ngurah, I Gusti Ketut Gejir, I Nyoman Gusti Ayu Marhaeni Hana Kristal Alamanda Septiara Harini, I Gusti Ayu Hartati, Ni Nyoman Henny Achjar, Komang Ayu Hikam, Ahdan Sayid I Dewa Made Sukrama I G. Wijana I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Gusti Putu Bagus Sasrawan Mananda I Ketut Adi Sugita I Ketut Mangku Budiasa I Ketut Muka I Ketut Suada I Ketut Suardana I Ketut Suatha I Komang Gede Wiryawan I Made Adikampana, I Made I Made Kardena I Made Mertanadi I Made Sukada I Made Sukarja I Made Walesa Putra I Nengah Kerta Besung I Nengah Sujaya I Nengah Wirakesuma, I Nengah I Nyoman Ariana I Nyoman Suarsana I Nyoman Sudiarta I Nyoman Sukma Arida I Nyoman Sunarta I Nyoman, suardina I Putu Sampurna I Putu Sudiarta I Wayan Adnyana I Wayan Mustika I Wayan Seriyoga Parta I. H. U Utama Ida Bagus Ngurah Swacita Iga Prassetyo Adji, Iga Prassetyo Ignatius Cahyanto INDAYATI LANYA Iwan Harjono Utama Karuni, Ni Kadek Khamid Yusuf Baehaqi, Khamid Yusuf Komang Januartha Putra Pinatih Kondra, I Wayan Korbinianus Feribertus Rinca Kumalasari, Ni Putu Putri Kusumajaya, Anak Agung Ngurah Laba, I Nyoman MAS DJOKO RUDYANTO Meitisrilatifatulain Fitriadewi Mariana Michael Haryadi Wibowo Mita Ekamelinda Mochamad Choirul Hadi Ngurah, I Gusti Ketut Gede Ngurah, IGK Gede Ni Kadek Ari Divania Widia Artha Ni Kadek Lyming Lestari Ni Ketut Suwiti Ni Luh Sustiawati Ni Luh Watiniasih Ni Made Ayu Aryati Dinarini Ni Made Inna Dariwardani Ni Made Ruastiti Ni Putu Ratna Sari Nur Habibah Nuria Fitrianti Putri Nyoman Dewi Pebryani NYOMAN SEMADI ANTARA Oktivia Chandra Mustika, Oktivia Chandra P. Sampurna Padmi, Luh Sri Anggayoni Julia Pitriyantini, Putu Eka Prabhadewi, Ni Putu Sriarta Pramesti, Kadek Diah Pratiwi, Ida Ayu Windhari Kusuma Putra, I Kadek Aldi Margareta Perdana Putu Agus Wikanatha Sagita Putu Ayu Sisyawati Putriningsih Putu Cahaya Semesta Putu Januari Ratna Apsari Putri Putu Sucita Yanthy R Susanti Raharjo, Anis Rahmasari, Ni Nyoman Putri Asri Rasdianah Rasdianah Remawa, Anak Agung Gede Rai Reny Navtalia Sinlae Rian Ka Praja Ribek, Nyoman Richard Christian Daud Ruspawan, Dewa Made Ruta, I Made Satriawati, Ni Nyoman Ayu Sipahutar, Ida Erni Siti Helmyati Sri Anggreni Lindawati Sri Wahyuni Suarya Putra, I Nyoman Agus Sudiantara, I Ketut Sudiantara, Ketut Suharsono, Hamong Sukoco, Hendro Sulisnadewi, Ni Luh Kompyang Sunatha Putra, Agus Surasak Jamnongsarn, Surasak Surya, I Kadek Adi Syamsul Alam Paturusi Tjokorda Sari Nindhia Totton, Mary Louise Victor Bangun Mulia Wahyu Hananto, Wahyu Wayan Tunas Artama Wayan Tunas Artama Wedri, Ni Made Widya Asmara Wilantari, Ni Nyoman Ayu Wulandari, Andi Dewi Wulandari, Kadek Dina Yan Ramona Yuli Darmawan, Yuli Yunita Sri Hastuti, Yunita Sri