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MCP-1 LEVELS AND ATYPICAL LYMPHOCYTES IN EARLY FEVER OF DENGUE VIRUS INFECTION WITH NON-STRUCTURAL PROTEIN 1 (NS-1) ANTIGEN TEST IN dr DARSONO HOSPITAL, PACITAN Agustiningrum, Indah; Nugraha, Jusak; Kahar, Hartono
Indonesian Journal of Tropical and Infectious Disease Vol. 8 No. 1 (2020)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v8i1.12696

Abstract

Dengue infection caused by DENV and transmitted by mosquitoes Aedes aegypti and Aedes albopictus is a major health problem in the world, including Indonesia. Clinical manifestations of dengue infection are very widely, from asymptomatic until dengue shock syndrome (DSS). DENV will attack macrophages and dendritic cells (DC) and replicate them. Monocytes are macrophages in the blood (±10% leukocytes). Macrophages produce cytokines and chemokines such as monocyte chemotactic protein-1 (MCP-1)/CCL2. The monocytes that are infected with DENV will express MCP-1, which will increase the permeability of vascular endothelial cells so that they have a risk of developing DHF/DSS. Macrophages and DC secrete NS1 proteins, which are the co-factors that are needed for viral replication and can be detected in the early phase of fever. The increased MCP-1 levels in dengue infection followed by an increase in the number of atypical lymphocytes indicate the arrival of macrophages and monocytes to the site of inflammation which triggers proliferation rather than lymphocytes. This is an observational analytical study with a cross-sectional design to determine the MCP-1 level in dengue infection patients with 1st until the 4th day of fever and the presence of atypical lymphocytes. Dengue infection was determined by rapid tests NS1 positive or negative and MCP-1 levels were measured using by ELISA sandwich method.MCP-1 level of sixty patients dengue infection NS-1 rapid positive or negative with 2nd until 4rt fever were significantly higher than healthy subjects (420.263±158,496vs29, 475±23.443;p=0.000), but there was no significant difference in subjects with DF, DHF or DSS (436,47±225,59 vs422,77±170,55vs 448,50±117,39; p =0.844). Atypicallymphosite differs significantly in healthy  subjects than subjects infected with DENV an average of 2% (p= 0,000). In conclusion, this shows the arrival of macrophages and monocytes to the site of inflammation, which triggers the proliferation of lymphocytes.
Comparative Diagnostic Value of Dengue Infection Using ELFA and Two Commercial Immuno-Chromatography Tests Nugraha, Jusak; Luminto, Dian; Saputri, Agatha Efrad
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 30 No. 2 (2024)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v30i2.2246

Abstract

Dengue Virus (DEN-V) can lead to a broad spectrum of clinical presentations, from mild symptoms to mortality. Based on the presence of antibodies, dengue infection is categorized into primary and secondary dengue. Early diagnosis is crucial for effective treatment of DEN-V infection. Non-Structural Antigen 1 (NS1) and dengue antibodies like immunoglobulin M (IgM) and immunoglobulin G (IgG) anti-dengue are standard assays used for dengue diagnosis. Various immunoassay methods, including Enzyme-Linked Fluorescent Assay (ELFA) and Immuno-Chromatographic Tests (ICT), are employed to detect these antigens and antibodies. This study aimed to compare the diagnostic value between ELFA and two commercial ICTs for detecting NS1 antigen, IgM/IgG anti-dengue. Seventy suspected dengue patients with fever lasting two to seven days at Premier Hospital, Surabaya were enrolled in this study. Blood serum samples from the individuals were tested for NS1 antigen and IgM/IgG anti-dengue using VIDAS® and two commercial ICTs (Boson and SD Bioline). The sensitivity of NS1 antigen assays using ELFA and two ICTs was calculated, as well as the agreement rate between ELFA and both ICTs. ELFA demonstrated high sensitivity (77.97%) for NS1 antigen detection in diagnosing dengue infection compared to two ICTs (76.27% and 45.45%). Using an automated system in ELFA can offer more excellent diagnostic value and objective results and determine the cut-off ratio of IgM/IgG antibodies. All of this comes at a cost comparable to ICT.
RELIABILITY OF AMYLOID DETECTION IMMUNOASSAY KITS FOR ALZHEIMERS DISEASE SCREENING: A PRELIMINARY STUDY Putri, Indah Aprianti; Ningrum, Emilna Mega; Noviana, Rachmitasari; Mariya, Silmi; Saepuloh, Uus; Retnani, Elok Budi; Hamdan, Muhammad; Nugraha, Jusak; Darusman, Huda Shalahudin
Jurnal Kedokteran Hewan Vol 18, No 3 (2024): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i3.31109

Abstract

Alzheimers Disease is a progressive neurodegenerative disease that damages the brain and is part of dementia. Alzheimers dementia is caused by various factors that until now the proper therapy is uncertain. Kit production as a diagnostic tool to support the development of screening for Alzheimers disease is a strategic effort. Screening based on detection with Enzyme-Linked Immunosorbent Assay (ELISA) can work effectively and accurately to optimize a specific antibody material or monoclonal antibody to the A42 peptide. This study aimed to research the reliability of the amyloid detection kit which was developed as an alternative screening test for Alzheimers disease compared to commercially available kits. Based on the results and interpretation of inter and intra-assay coefficients, the in-house ELISA kit has comparable A42 detection to the commercially available ELISA kit
OPTIMATION OF PLATELET RICH PLASMA PRODUCTION PROTOCOL USING DOUBLE CENTRIFUGATION METHODS Nugraha, Hans Kristian; Hernaningsih, Yetti; Nugraha, Jusak
Folia Medica Indonesiana Vol. 51 No. 3 (2015): July - September 2015
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (42.569 KB) | DOI: 10.20473/fmi.v51i3.2832

Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable according to the literature is a double centrifugation method. The purpose of this research is to produce an optimization of the double centrifugation method. This study uses experimental data obtained by researchers at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments generally were conducted on stored blood obtained from the blood bag from PMI with CPD anticoagulant. Optimization of double centrifugation method to produce PRP with optimum platelet count could be obtained by using an "I"-shaped tube as well as other specialized equipment which are currently not available in standard clinical pathology laboratory, and also fresh blood. The resulting increase amounted to 4.11 times with the platelet count of 1.152 million.
CORRELATION BETWEEN THE USE OF ALBUMIN INFUSION AND CONCENTRATIONS OF SERUM ALBUMIN, PROINFLAMMATORY CYTOKINES (TNF-, IL6) AND SOFA SCORE IN SEPTIC PATIENTS Utariani, Arie; Prasetyo, Budi; Nugraha, Jusak
Folia Medica Indonesiana Vol. 52 No. 4 (2016): OCTOBER - DECEMBER 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.452 KB) | DOI: 10.20473/fmi.v52i4.5481

Abstract

Sepsis is a major problem causing high morbidity and mortality in patients who hospitalized in intensive care. In sepsis, there is a change in the distribution of albumin in the intravascular and extravascular compartment, so hipoalbumin is occured. Using albumin in septic patients from several studies that have been done are still controversial. Analyzing albumin infusion with changes in levels of albumin, cytokines and SOFA score in septic patients in intensive care dr. Soetomo. The design of this study is a prospective longitudinal observational. The study was conducted in the intensive care unit or ICU dr. Soetomo in February 2015. The inclusion and exclusion criteria were determined that obtained 15 research subjects. They were examined with serum albumin and proinflammatory cytokines as well as evaluating the SOFA score. Then, it measure with ANOVA test methods. Infusion of albumin positively correlated with increased levels of serum albumin and a negative correlation with the levels of IL6, TNF-a and SOFA score. It also found a correlation between changes in levels of albumin with changing levels of IL6 (p <0:01) and changes in SOFA score (p <0.05).
Lower Plasma β-Amyloid 1-42 Levels in Amnestic Mild Cognitive Impairment Compared to Healthy Individuals Cynthia Cynthia; Jusak Nugraha; Muhammad Hamdan; Hari Basuki Notobroto; Silvia Francina Lumempouw; Rahajuningsih Dharma
The Indonesian Biomedical Journal Vol 16, No 6 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i6.3404

Abstract

BACKGROUND: Amnestic mild cognitive impairment (aMCI) is strongly associated with an increased risk of progression to Alzheimer’s disease (AD). In AD, cerebrospinal fluid (CSF) β-Amyloid 1-42 levels are known to decrease, a pattern which is also observed in aMCI. While in AD, apolipoprotein E (ApoE) ε4 allele is known to be a genetic risk factor, the role of ApoE ε4 allele in modulating plasma β-Amyloid 1-42 levels in aMCI remains unclear. Therefore, this study was performed to evaluate plasma β-Amyloid 1-42 levels in aMCI patients compared to cognitively healthy individuals and investigate its association with ApoE ε4 allele.METHODS: A cross-sectional study involving 57 aMCI and 54 cognitively healthy control (HC) subjects was performed. Blood samples were taken from subjects from both groups for measurement of the plasma β-Amyloid 1-42 and ApoE ε4 allele. The plasma levels of β-Amyloid 1-42 were measured using an enzyme-linked immunosorbent assay (ELISA) methods, while the ApoE ε4 allele genotyping was conducted using polymerase chain reaction (PCR) techniques.RESULTS: Plasma β-Amyloid 1-42 in individuals with aMCI (23.9 pg/mL) was significantly lower than that in HC (25.3 pg/mL) with cut-off value of 24.6 pg/mL (AUC: 70.8%; 95% CI: 61.1–80.5%; p<0.001) sensitivity of 64.8%, and specificity of 71.9%. There was no significant association between plasma β-Amyloid 1-42 and the ApoE ε4 allele. However, plasma β-Amyloid 1-42 in ε4 carriers were lower than in ε4 non-carriers.CONCLUSION: Lower plasma β-Amyloid 1-42 levels were observed in aMCI patients compared to cognitively healthy individuals, suggesting its potential as a biomarker for identifying aMCI.KEYWORDS: blood biomarkers, amyloid beta peptides, amnestic mild cognitive impairment (aMCI)
Hubungan antara Kadar Mieloperoksidase (MPO) dan Kejadian Sindroma Koroner Akut (SKA) Jusak Nugraha; Citra Indah Setyaningrum; M. Aminuddin
JURNAL WIDYA MEDIKA Vol. 3 No. 1 (2015)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v3i1.774

Abstract

Beberapa juta pasien nyeri dada dicurigai menderita Sindroma Koroner Akut (SKA) tetapi hanya sekitar 10% yang didiagnosis dengan Infark Miokard Akut (IMA). SKA merupakan kumpulan gejala akibat gangguan aliran darah ke jantung yang terdiri dari infark miokard akut (IMA) disertai peningkatan segmen ST (STEMI), IMA tanpa peningkatan segmen ST (NSTEMI) dan angina pektoris tak stabil (UA). Mieloperoksidase (MPO) merupakan suatu enzim yang berperan dalam terjadinya destabilisasi dan ruptur plak yang meningkat lebih dini dalam waktu 2 jam sesudah serangan IMA sehingga dapat dipakai sebagai penanda awal terjadinya SKA tanpa bergantung adanya bukti nekrosis miokard. Penelitian ini bersifat analitik observasional dengan rancangan cross sectional. Sampel penelitian berupa serum 40 pasien dengan keluhan utama nyeri dada yang datang ke UGD RSUD dr Soetomo Surabaya. Analisa data dilakukan dengan korelasi Pearson dan nilai diagnostik dengan tabel 2x2. Hasilnya diketahui pasien nyeri dada dengan dugaan SKA 27 orang (67,5%) sedangkan pasien nyeri dada yang non-SKA 13 orang (32,5%). Pasien SKA dengan jenis kelamin laki-laki 20 orang (74,07%), perempuan 7 orang (25,93%). Terdapat perbedaan yang signifikan kadar MPO antara kelompok pasien SKA (STEMI, NSTEMI, UA) yaitu sebesar 986,48 ng/ml dan non-SKA 381,08 ng/ml (p
Hubungan antara Kadar Soluble CD4O Ligand (sCD4OL) dengan Kejadian Sindroma Koroner Akut (SKA) Jusak Nugraha; Myrza Elita; M. Aminuddin
JURNAL WIDYA MEDIKA Vol. 4 No. 1 (2018)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v4i1.1781

Abstract

Introduction: Acute coronary syndrome (ACS) is manifestation of coronary heart disease (CHD), which is the leading cause of death in Indonesia. CD40 ligand (CD40L) stored in alpha granule of platelet will be translocated immediately to the surface when plaletet is active, and then released from the surface as soluble CD40 ligand (sCD40L). Soluble CD40 ligand (sCD40L) has role in connecting the inflammatory process, atherosclerosis, and thrombosis. The aim of this study is to study correlation between sCD40L level and ACS in patients with chest pain. Methods: Subject of this study was 40 patients with chest pain that came to emergency ward of RSUD dr. Soetomo Surabaya. Patients were divided into two groups based on the diagnosis ACS and non ACS, which was established with electrocardiography(ECG) and Troponin-T level. Patients’sera were examined sCD40L level using enzyme-linked immunosorbent assay (ELISA) kit from Quantikine®. Results: Twenty six (65%) were diagnosed as ACS, and 14 (35%) were non ACS. ACS diagnosis consists of ST-segmen elevation myocardial infarction (STEMI), non-ST-segmen elevation myocardial infarction (NSTEMI), and unstable angina (UA), with the highest proportion was STEMI in 15 (57%). The mean of sCD40L level in ACS patients (5.45 ± 3.70 ng/mL) was significantly higher compared with non ACS patients (1.97 ± 1.33 ng/mL) (p
Perbedaan Jumlah Trombosit Platelet Rich Plasma Yang Menggunakan Tabung Natrium Sitrat Dan Tabung ACD-A Sheila Clarissa; Jusak Nugraha; Triagung Ruddy
JURNAL WIDYA MEDIKA Vol. 5 No. 1 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33508/jwm.v5i1.1996

Abstract

Background: PRP is a relatively new field and there is still minor researches that are usage. However, PRP can be applied to a lot of various fields. This study willmake a comparison between tubes that have sodium citrate anticoagulant and tubes that have ACD-A anticoagulants, to be able to find out which of the two are able to produce a higher number of PRPs. Objective: To obtain the anticoagulants that produce more platelet countsand to know the difference in the platelet counts in PRPs found in tubes that have sodium citrate anticoagulants and tubes that have ACD-A anticoagulants. Method: This study was an experimental laboratorium research with an equivalent control group design. Data is normally distributed, so that the parametic statistical analysis test uses Paired T-Test. Results: From 32 respondents, there was an increase in both anticoagulant tubes, where the average increase in platelet counts in PRP using sodium citrate tubes was 337593.75 / μl and the standard deviation was 158795.437 whereas, the average increase in platelet counts in PRP using ACD-A tubes was 909062.50 / μL and the standard deviation is. 284336.005. The difference in platelet counts in PRP using sodium citrate tubes and ACD-A tubes was stated to be significant because the result of the Paired T-Test analysis showed Sig (2-Tailed) = 0,000 (p
THE EFFECT OF ASTAXANTHIN ON TUMOR NECROSIS FACTOR ALPHA (TNF-α) AND INTERLEUKIN 10 (IL-10) EXPRESSION IN UV-B-INDUCED RATS MODEL Akhmad Setyo Rahman; Theresia Indah Budhy; Jusak Nugraha; Nur Lailatul Fadhilah
Folia Medica Indonesiana ON PROGRESS
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The skin, the largest organ in the human body, serves as a protective barrier against external factors. Exposure to UV-B radiation accounts for approximately 90% of skin damage, leading to aging characterized by dryness and wrinkles. Proper nutrition has been associated with skin repair and delayed aging. Astaxanthin, known for its pharmacological properties including anti-cancer, anti-diabetic, anti-inflammatory, and antioxidant activities has garnered attention for its ability to improve damaged skin when administered as a daily supplement. This study investigated the roles of TNF-α and IL-10 as inflammatory markers in skin damage and repair, which remain underexplored. A true experimental randomized posttest-only control group design was employed using 24 male white rats divided into four groups: (1) normal group (N), untreated rats; (2) control group (C), rats administered with olive oil (5 ml/kg BW); (3) treatment group 1 (P1), rats exposed to UV-B and administered with olive oil (5 ml/kg BW); and (4) treatment group 2 (P2), rats exposed to UV-B and administered with astaxanthin (10 mg/kg BW) combined with olive oil (5 ml/kg BW). ANOVA and Games-Howell post-hoc tests revealed significant differences (p < 0.05) among groups, except between the control (C) and normal (N) groups. Astaxanthin (10 mg/kg BW) demonstrated both curative and protective effects by reducing TNF-α expression (pro-inflammatory) and increasing IL-10 expression (anti-inflammatory) in UV-B-induced rats. These findings highlight astaxanthin's potential as a therapeutic agent for UV-B-induced skin damage.
Co-Authors Abdul Hafid B Agung Dwi Wahyu W Agung Dwi Wahyu Widodo Agustiningrum, Indah Akhmad Setyo Rahman Alfia Andriyani Amellya Octifani aminuddin, mohammad Anak Agung Istri Sri Wiadnyani Andrianto Anita Budiarti Anna Roosdiana Annette d’Arqom, Annette Anton Yuntarso Aryati Aryati Aryati Aryati Ashon Sa'adi Ashon Sa’adi Ashon Sa’adi Audrey Gracelia Riwu Awalia Awalia Ayu Imamatun Nisa Bambang Purwanto Bastian Bastian Bastian Bastian Beatrix, Stephanie Besin, Valentinus Betty Agustina Betty Agustina Tambunan Buana, Dwi Candra Budi Prasetyo Budi Utomo Budiutari, Ni Nyoman Budy, Theresia Indah Callixte, Cyuzuzo Caroline Caroline Ch. Destri Wiwis Wijayanti Christina Destri Citra Indah Setyaningrum Cynthia Cynthia Ayu Permatasari Cynthia Ayu Permatasari Cynthia Cynthia Cynthia Cynthia Cyuzuzo Callixte Darmadi, Epriyanto T Dheasy Herawati Diyan Wahyu Kurniasari Djoko Agus Purwanto Dominicus Husada Dwi Aris Agung Nugrahaningsih Dwi Liliek Kusindarta Dwiyanti Puspitasari, Dwiyanti Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim Eddy Mart Salim, Eddy Mart Edhi Rianto Elok Budi Retnani Elvan Dwi Widyadi Erfina Lim Erna R Tobing Erwin Astha Triyono Esthy Poespitaningtyas Febtarini Rahmawati Ferdy R. Marpaung Ferdy Royland Marpaung Foe, Kuncoro Francisca Srioetami Tanoerahardjo Francisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransisca Srioetami Tanoerahardjo Fransiska Fransiska Fransiska Sri Oetami Gede Arie Wijaya Handayani, Luh Putu Trys Monika Hanik Badriyah Hidayati,* Mohammad Hasan Machfoed,* Kuntoro,** Soetojo,*** Budi Santoso,**** Suroto,***** Budi Utomo****** Hans Kristian Nugraha, Hans Kristian Hantoro Gunawan Hari Basuki Notobroto Hari Basuki Notobroto Hari Setiono Harianto Notopuro Hartono Kahar, Hartono Hendy Wijaya Herin Mawarti Heru Setiawan Hery Priyanto Hevi Wihadmadyatami Holland Lydia Marpaung Huda Shalahudin Darusman I Made Andika Bara Kusuma Idha Kusumawati Ilham Ilham Irvan Ipandi Januarti, Catur Ifda Joewono Soeroso John Wiwin Julious Julious julious, julious Kadeq Novita Prajawanti Kasiyati, Menik Kemas Ya'kub Rahadiyanto Kemas Ya'kub Rahadiyanto Kemas Ya’kub Rahadiyanto kurniati, nova Lisa Soegianto Lumempouw, Silvia Luminto, Dian Lutfi Dewanda Nugroho M. Aminuddin M. Aminuddin M. Irsan Saleh Mangestuti Agil Maya E. Roring Meiti Muljanti, Meiti Mohammad Aminuddin Mohammad Hasan Machfoed Muhammad Amin Muhammad Amminuddin Muhammad Hamdan Muhammad Hamdan Muhammad Irsan Saleh Muhammad Nazarudin Munawaroh Fitriah Munawaroh Fitriah Munawaroh Fitriah Myrza Elita Naesilla Naesilla Nico Jafet Ningrum, Emilna Mega Ninik Asmaningsih Soemyarso Notopuro, Paulus Budiono Nova Kurniati Nova Kurniati Nova Kurniati Noviana, Rachmitasari Nugraha, Hans K Nur Hayati Nur Lailatul Fadhilah Oetami, Fransisca Sri Oryza Chrisantia Oski Illiandri Patria Dewi Pande Pratama, Dyah Ayu Oktaviani A Prihantika S., Sabrina Pudji Lestari Purwanta, Marijam Purwoko, Agus Puspitasari, Yessy Putri, Indah Aprianti Rahadiyanto, Kemas Ya'Kub Rahajuningsih Dharma Rahajuningsih Dharma Rahayu Nofita Sari Rahma Indah Pratiwi Ravell Hansen Untono Rendy, Achmad Nur Retno Indrawati Rifa’I, Muh Husni Risky V. Prasetyo Rochmah Kurnijasanti Roedi Irawan Rusli, Musofa Ryzky Widi Atmaja S. Soedarsono S.Pd. M Kes I Ketut Sudiana . Sabri Prihantika Sabrina Prihantika Saleh, M Irsan Saputra, Angky Saputri, Agatha Efrad Senny Yesery Esar Setiawan, Lidwina Tri Kristanti Shahzad Shoukat Sheila Clarissa Shoukat, Hamad Shoukat, Shahzad SILMI MARIYA Silvia F. Lumempouw Silvia Francina Lumempouw Sinansari, Restry Siti Nur Husnul Yusmiati Siti Rahmawati Soedarsono Soedarsono Soedarsono Soedarsono Soelistijo, Soebagijo Adi SP Edijanto Srikanth Karnati Süleyman Ergün Suryawan, I Gde Rurus Susi Oktaviani Tanoehardjo, Francisca Srioetami Tanoehardjo, Francisca Srioetami Tanoerahardjo, Francisca Srioetami Tedja, I G.A. Wiradari Theresia Indah Budhy Theresia Indah Budhy Sulisetyawati Triagung Ruddy Tuyishimire Irene Utariani, Arie UUS SAEPULOH Wahyu Dewi Tamayanti, Wahyu Dewi Wahyu Setiani Wibowo Wahyu Setiani Wibowo Winthoko, Eka Nora Vitaloka Aprilia Putri Wulandari, Dian Novita Wuryanto Hadinugroho Yetti Hernaningsih Yoes Prijatna Dachlan Yohanes Adrian Kapri Negara Yuani Setiawati Yudy Tjahjono Yufita Ratnasari Wilianto Yulia Nadar Indrasari Zen Hafy