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All Journal Jurnal Ilmu Ternak Media Kedokteran Hewan Jurnal Medik Veteriner Jurnal Layanan Masyarakat (Journal of Public Service) Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Current Biomedicine Jurnal Agro Veteriner
Tita Damayanti Lestari
Department Of Reproduction, Faculty Of Veterinary Medicine, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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The usage of cattle rumen contents in commercial quail feed towards the production and haugh unit (HU) of eggs (Coturnix coturnix japonica) Joel Jeevan Raj S/O Yogarajah; Tita Damayanti Lestari; Mohammad Anam Al Arif; Widya Paramita Lokapirnasari; Sri Hidanah; Nove Hidajati
Jurnal Agro Veteriner Vol. 8 No. 1 (2024): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i1.64989

Abstract

The purpose of this study was to determine the effect of rumen content fermentation on quail bird ration. The parameters measured were the production of egg weight and haugh unit. In total 24 quail birds were used with 4 treatments and 6 repetitions. Treatments were consists of T0(formulated feed without fermented rumen content), T1(formulated feed with 5% fermented rumen content), T2(formulated feed with 10% fermented rumen content), T3(formulated feed with 15% fermented rumen content). Samples are observed on the last day to determine the egg quality with the egg weight and haugh unit. The results showed that there are significant differences effected on the egg weight and haugh unit.
The influence of using fermentated rumen content at quail feed to the protein of yolk and albumen of quail eggs Gesa Cantya Primatara; Mohammad Anam Al Arif; Tita Damayanti Lestari; Widya Paramita Lokapirnasari; Sri Hidanah; Suharsono, Suharsono
Jurnal Agro Veteriner Vol. 8 No. 1 (2024): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v8i1.64994

Abstract

Ruminal content is one of slaughterhouse waste that has not been utilized optimally, in fact there are cases where ruminal content are thrown away haphazardly, causing environmental pollution. The nutritional value of ruminal content is relatively high which is caused by the imperfect nutrition absorption do not have much difference with the original nutritional value coming from the cattle feed itself. nutritional value in ruminal content is 8.86% of protein, 2.6% of fat, 28.78% of fiber, 0.55% of phosphor, 18.54% of ash and 10.92% of water content. The aims of this research was to find out whether the usage of fermentation cattle ruminal content in quail’s feed ransom can influence the protein content inside the yolk and albumen of an egg. This research used fermentation cattle ruminal content and divided into four treatments. The first treatment (T0) was only giving formula feed. The second treatment (T1) was Formulation feed (5% fermentated rumen content). The second treatment (T2) was Formulation feed (10% fermentated rumen content). The third treatment (T3) was Formulation feed (15% fermentated rumen content). The experiment design was Completely Randomized Design. 
Estrus response in dairy cows surviving foot and mouth disease given Superbooster and Immunobooster Zulfanisa, Rizky Asrin; Rahmi Sugihartuti; Jola Rahmahani; Mohammad Anam Al Arif; Tita Damayanti Lestari; Ali Agus; Mohammad Sofi’ul Anam
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 3 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i3.2024.139-145

Abstract

This study aims to determine the effect of adding Superbooster and Immunobooster on the estrus response of dairy cows after foot and mouth disease (FMD) infection. This study used 18 dairy cows that recovered from FMD based on veterinarian examination. Cows were divided into three groups (T0, T1 and T2). In the control group (T0), cows were fed standard feed. Standard feed consisted of forage (10% of body weight, daily) and concentrate. In the T1 group, cows were fed standard feed added with Superbooster (40 g daily), and in the T2 group cows were fed standard feed added with Superbooster (40 g daily) and Immunobooster (800 g daily). This treatment was carried out for 42 days. The results showed that the estrus rate in T1 and T2 were both 100%, which was higher compared to the control (16.67%). The onset of estrus was shorter (p <0.05) compared to the duration of the last estrus until the start of treatment. Meanwhile, the duration of the last estrus until the day of treatment and onset of estrus between treatment groups were not significantly different (p <0.05). It could be concluded that Superbooster and Immunobooster could stimulate estrus on dairy cows after FMD.
COUNSELING ON REPRODUCTIVE DISORDERS MANAGEMENT IN FEMALE BEEF CATTLE LIVESTOCK GROUPS IN DALEGAN VILLAGE, PANCENG DISTRICT, GRESIK REGENCY Suprayogi, Tri Wahyu; Susilowati, Suherni; Hermadi, Herry Agoes; Al Arif, M Anam; Hernawati, Tatik; Mulyati, Sri; Suwanti, Lucia Tri; Maslachah, Lilik; Kurnijasanti, Rochmah; Lestari, Tita Damayanti; Effendi, Mustofa Helmi; Hernanda, Ary Setya; Supriyadi; Saputra, Rakan Mahiid; Dewi, Aprilia Kurnia
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 9 No. 1 (2025): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v9i1.2025.026-038

Abstract

Management of reproductive disorders in female beef cattle (Bos taurus) is a vital step in enhancing livestock productivity and animal welfare. This educational activity was conducted in Dalegan Village, Panceng District, Gresik Regency, aiming to assist farmers in improving cattle reproductive performance through feed technology and supporting the development of modern farming practices. The methods applied included counseling, practical field demonstrations, and interactive discussions with participants, presented in a qualitative descriptive format. The results indicated an improvement in farmers' knowledge of the causes, clinical signs, and management steps for reproductive disorders such as anestrus, dystocia, and endometritis, as evaluated through pre-test and post-test assessments. Additionally, the activity provided guidance on the importance of feed management, cattle reproductive health, and modern farm management. In conclusion, this counseling successfully increased farmers' awareness of cattle reproductive health, which is expected to support the productivity of beef cattle farming in the region.
A study on the beneficial effect of advocating prophylactic spaying of young nulliparous bitches to prevent pyometra Sharveihn Sathindren; Suryo Kuncorojakti; Suzanita Utama; Tita Damayanti Lestari; Wiwik Misaco Yuniarti
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 1 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i1.2025.8-15

Abstract

This study aimed to evaluate the potential benefits of advocating elective spaying (ES) in young nulliparous bitches as a preventive measure for pyometra, a severe and potentially life-threatening condition. Specifically, the study examined whether elective spaying, which eliminates the risk of pyometra and its associated complications, offers significant advantages over the treatment of pyometra once it develops. Generally, the short- and long-term risks associated with elective spaying are minimal when compared to the significant complications and dangers posed by pyometra. A retrospective open-ended study was conducted, collecting data from veterinary clinics across Penang Island using a structured questionnaire. Information was gathered primarily from medical records and interviews with veterinarians and pet owners when data were missing or unclear in the records. The study compared 30 bitches of various breeds who underwent elective spaying with 30 bitches who underwent emergency ovariohysterectomy (EOH) due to pyometra. A standard set of outcomes was evaluated to assess and compare complications. The results showed that the elective spaying group experienced only minor or trivial complications, and owners incurred significantly lower costs compared to those whose bitches underwent emergency surgery for pyometra, which was associated with more severe complications and substantially higher costs. Based on these findings, the study concluded that elective spaying is a safe and cost-effective prophylactic measure against pyometra.
Role of Cryoprotectants in Preventing Quality Loss of Goat Oocytes after Warming by Examining Insulin-Like Growth Factor-1 and Bone Morphogenetic Protein-15 Expression Shabira, Zahra; Widjiati, Widjiati; Rimayanti, Rimayanti; Lestari, Tita Damayanti; Safitri, Erma; Mafruchati, Maslichah; Hendrawan, Viski Fitri
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.175-182

Abstract

This research aimed to assess the efficacy of ethylene glycol-sucrose as an alternative cryoprotectant compared to commercial cryoprotectants by focusing on the expression of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-15 (BMP-15) in goat oocyte cumulus complexes after warming with identification using the immunocytochemical analysis. Maturated oocytes were divided into 3 groups, i.e., (C) the control group followed by an immunocytochemical examination, and two treatment groups, i.e., (P1) was exposed to a commercial cryoprotectant for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen, and (P2) was exposed to 30% ethylene glycol and 1 M sucrose for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen. The results revealed divergent responses in IGF-1 (CG = 9.00 ± 3.00; P1 = 9.50 ± 3.20; P2 = 4.67 ± 0.94) and BMP-15 (CG = 10.50 ± 3.35; P1 = 9.50 ± 2.69; P2 = 5.50 ± 3.64) expression patterns, possibly influenced by the two cryoprotectant abilities and oocytes performing their permeabilities to cryoprotectant solution. We concluded that each cryoprotectant is necessary to mitigate the adverse impact on gene expression in oocytes so that we can use it for reproductive technologies.
THE EFFECT OF BREED AND BODY CONDITION SCORE ON OVARIAN WEIGHT, NUMBER OF FOLLICLES, AND OOCYTE QUALITY IN RETIRED COWS Putri, Natalie Lovena Pramono; Ikhsan, Dilla Amalia Bilqis; Raharjo, Dadik; Khairullah, Aswin Rafif; Lestari, Tita Damayanti; Mustofa, Imam; Rimayanti, Rimayanti; Safitri, Erma; Amrullah, Muhammad Fajar; Ilmi, Laily 'Ulya Nurul; Pasang, Gabriel Sampe; Supriyadi, Supriyadi
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.44392

Abstract

The aim of this study was to determine whether breed and body condition score (BCS) can influence ovarian weight, follicle number, and oocyte quality. This study used 90 ovaries from 15 Friesian Holstein crossbreed, 15 Ongole crossbreed, and 15 Limousin crossbreed cows obtained from an abattoir. Before the ovaries were taken, the BCS of the cows was determined. The BCS system used in this study is BCS with a scale of 1 to 5. Ovaries were weighed, follicles were measured and grouped by size (2 mm, 2-6 mm, 6 mm) then counted, oocytes were aspirated from the follicles then observed under a microscope. Oocyte quality was observed and then grouped into grade A, B, C, D based on cumulus oocyte complex (COCs). Data were analyzed using two way ANOVA and chi square test. The results of the analysis showed that breed did not influence ovarian weight, but influenced the number of follicles, and oocyte quality. In Friesian Holstein crossbreed cows, BCS affects ovarian weight, follicle number, and oocyte quality. In Ongole crossbreed cows, BCS had no effect on ovary weight, but did affect follicle number and oocyte quality. In Limousin crossbredcows, BCS had no effect on ovary weight and follicle number, but did affect oocyte quality.
Factors associated with repeat breeding in Holstein Friesian cows at the Tani Wilis Village Cooperative Unit, Sendang District, Tulungagung Regency, Indonesia Dimas Kunto Satrio; M’ Izi Kumala Lazuardi Sultoni; Tasya Apritalia Putri; Soeharsono Soeharsono; Dadik Rahardjo; Djoko Legowo; Gandul Atik Yuliani; Iwan Sahrial Hamid; Mohammad Anam Al-Arif; Sunaryo Hadi Warsito; Pudji Srianto; Sri Pantja Madyawati; Tita Damayanti Lestari; Wurlina Wurlina
Ovozoa: Journal of Animal Reproduction Vol. 14 No. 2 (2025): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v14i2.2025.67-75

Abstract

This study aimed to evaluate the association of factors such as nutrition, thermal humidity index (THI), age, and lactation period with the occurrence of repeat breeding of dairy cows. The sample consisted of Holstein Friesian cows aged 1-11 years, with lactation periods ranging from 1 to 7 months, currently in lactation, and experiencing repeat breeding (inseminated three or more times without conception, despite exhibiting normal estrous cycles and intervals). The results showed that feeding practices by farmers did not significantly differ (p >0.05) in relation to the incidence of repeat breeding. THI values recorded in several barns also showed no significant differences (p >0.05), with the cows experiencing light to moderate heat stress. Similarly, the frequency of artificial insemination and the estrous cycle intervals remained within the normal range. The highest incidence of repeat breeding was observed in cows aged 2.5 to 5.5 years, accounting for 78.13%, and during lactation periods of 1-3 months, accounting for 81.26%. It can be concluded that there were no significant differences in the provision of forage and leguminous feed between normal cows and those with repeat breeding, and the crude protein content of forage did not influence its occurrence. Additionally, there was no indirect relationship between THI and repeat breeding. However, the incidence of repeat breeding tended to increase in cows aged 3-4 years, and during the second and third lactation periods.
The Effect of Kemangi Leaf (Ocimum Basilicum) Extract in The Egg Yolk Skim Milk Diluent on The Plasma Membrane and Motility of Boerawa Goats' Sperm Farah Ghifara Hafidha; Utomo, Budi; Soeharsono; Lestari, Tita Damayanti; Hidajati, Nove; Hermadi, Herry Agoes
Media Kedokteran Hewan Vol. 36 No. 3 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i3.2025.273-281

Abstract

The purpose of this research was to determine the effect of kemangi leaf (Ocimum basilicum) extract in the egg yolk skim milk diluent on the plasma membrane and motility of Boerawa goats' sperm at room temperature. The research used fresh samples of Boerawa goat semen collected by artificial vagina. The experimental design used was a completely randomized design, divided into four treatments with six replications. The treatments were the addition of kemangi leaf extract at the levels of 0% (P0), 1% (P1), 2% (P2), and 3% (P3). Diluted semen was stored at room temperature and observed at hours 0, 1, 2, and 3. Analysis of the data using Multivariate Analysis of Variance (MANOVA), then proceed to the Duncan Test to determine significant differences between treatments. The results showed that there was no significant difference (p > 0.05) in sperm plasma membrane, but a significant difference (p < 0.05) in sperm motility was observed when kemangi leaf extract was added. However, the addition of kemangi leaf (Ocimum basilicum) extract tends to provide a better value than without it. The addition of kemangi leaf extract at a concentration of 2% yields the highest results in sperm plasma membrane integrity and sperm motility of Boerawa semen at room temperature.
Detection of Anti-Müllerian Hormone (AMH) mRNA in Serum and Ovarian Tissue of Local Indonesian Cattle Using EvaGreen-based RT–qPCR Dikman, Dicky Mohammad; Puspitasari, Heni; Waluyo, Seagames; Kurniawan, Muhammad ‘Ahdi; Supriyadi, Supriyadi; Srianto, Pudji; Luqman, Epy Muhammad; Suprayogi, Tri Wahyu; Madyawati, Sri Pantja; Safitri, Erma; Lestari, Tita Damayanti
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.382-397

Abstract

Anti-Müllerian hormone (AMH) has been identified as a potential biomarker for assessing ovarian reserve and reproductive capacity in cattle, with high heritability and relatively stable expression. However, studies detecting AMH mRNA in bovine serum remain scarce due to the matrix’s inherently low RNA yield, susceptibility to degradation, and potential presence of PCR inhibitors. This study provides an exploratory validation of AMH mRNA detection in serum — a challenging matrix compared with ovarian tissue — using EvaGreen-based reverse transcriptase–quantitative PCR (RT–qPCR). Specific primers for the AMH and β-actin genes were designed in silico and validated through melting curve analysis and linearity testing. The results showed high amplification efficiency (AMH: 100.2%, R² = 0.994; β-actin: 109.1%, R² = 0.996), with specific amplification of both targets. AMH detection in serum samples was successful in some samples, while the β-actin gene was consistently amplified as a reference gene. Despite the low RNA quality from serum and the presence of organic contaminants, the method demonstrated its feasibility for detecting AMH transcripts in a minimally invasive manner. Physiologically, AMH levels positively correlate with antral follicle count, superovulation success, and embryo quality, and are sensitive to heat stress and other environmental factors. These findings provide a foundational basis for developing molecular diagnostic approaches based on AMH gene expression in cattle reproductive management programs and support the future development of efficient, accurate, and context-specific biomolecular-assisted selection technologies for tropical livestock systems.
Co-Authors Abdul Samik, Abdul Adiana Mutamsari Witaningrum Aksono HP., Eduardus Bimo Al Arif, M Anam Al arif, Mohammad Anam Ali Agus Amrullah, Muhammad Fajar Aryaloka, Suhita Ayuti, Siti Rani Belgania, Ristaqul Husna Berliana, Erprinanda Galuh Boedi Setiawan Budi Utomo Budiarto Budiarto Budiarto Chandramohan, Koshini Charles, Vanessa Ann Chusniati, Sri Cindy Ercha Aulia Putri Dadik Rahardjo Dewi, Aprilia Kurnia Dikman, Dicky Mohammad Dimas Kunto Satrio Djoko Legowo Eka Pramyrtha Hestianah, Eka Pramyrtha ERMA SAFITRI Erma Safitri Faiqoh, Berliana Elok Farah Ghifara Hafidha Frida Aulya Arningdiah Gandul Atik Yuliani Gesa Cantya Primatara Hariadi, Mas'ud Hasib, Abdullah Heni Puspitasari Hermin Ratnani Hernanda, Ary Setya Herry Agoes Hermadi Hidajati, Nove Hidanah, Sri Hussain, Muhammad Asif Ikhsan, Dilla Amalia Bilqis Ilmi, Laily 'Ulya Nurul IMAM MUSTOFA Ismudiono Ismudiono, Ismudiono Iwan Sahrial Hamid Joel Jeevan Raj S/O Yogarajah Khairullah, Aswin Rafif Kirana, Sabila Citra Kuncorojakti, Suryo Kurniawan, Muhammad ‘Ahdi Legowo, Djoko Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Mafruchati, Maslichah Meydilasari, Nanda Irsa Mirni Lamid Mohammad Sofi’ul Anam Moses, Ikechukwu Benjamin Mufasirin Muhammad Al-Syafiq bin Abdul Halim Mustofa Helmi Effendi M’ Izi Kumala Lazuardi Sultoni Nove Hidajati Nuurin Ajrin Karim Pasang, Gabriel Sampe Permatasari, Dian Ayu Poedji Hastutiek Pristian Devon Martyas Wahyuningrum Pudji Srianto Putri, Natalie Lovena Pramono Raharjo, Dadik Raharjo, Hartanto Mulyo Rahmahani, Jola Rahmatullah, Aldin Akbar Rahmawati, Mitha Ardila Rahmi Sugihartuti Ratna Damayanti Rehman, Saifur Rimayanti Rimayanti Rimayanti Rochmah Kurnijasanti Saputra, Rakan Mahiid Sari, Aulia Puspita Seagames Waluyo, Seagames Setiawan Koesdarto Shabira, Zahra Sharveihn Sathindren Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Mulyati Sri Pantja Madyawati Sri Pantja Madyawati, Sri Pantja Suharsono Suharsono Suherni Susilowati Supriyadi Supriyadi Supriyadi Suzanita Utama Tasya Apritalia Putri Tatik Hernawati Tjuk Imam Restiadi Tri Wahyu Suprayogi Triagil, Annisa Lita Trilas Sardjito Tsaniya Atikahani Shabirah Viski Fitri Hendrawan Wahyuni, Retno Sri Warsito, Sunaryo Hadi Widjiati Widjiati, Widjiati Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti Wurlina, W Yulianna Puspitasari Yunus, Muchammad Zulfanisa, Rizky Asrin