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EVALUATING THE DIVERSITY AND ABUNDANCE OF MARINE FISH IN THE MAKASSAR STRAIT, INDONESIA THROUGH ENVIRONMENTAL DNA (EDNA) ANALYSIS Rukminasari, Nita; Hidayani, Andi Aliah; Moka, Wilma J.C.; Arbit, Nur Indah Sari; Andriyono, Sapto; Parenrengi, Andi
Jurnal Ilmu Kelautan SPERMONDE VOLUME 10 NUMBER 2, 2024
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jiks.v10i2.32405

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The recent adoption of environmental DNA (eDNA) represents an innovative method for assessing the presence of aquatic vertebrate species, providing a relatively straightforward approach with significant implications for conservation biology. In our investigation, we employed eDNA metabarcoding to explore the diversity of fish in the Makassar Strait. We collected eDNA from samples taken both at the surface and from the water column (15 m depth) at two specific locations within this region. The reliability of the MiFish-U primer set methodology in estimating fish diversity in the Makassar Strait was assessed. In a single survey, based on four water samples from the Makassar Strait, we successfully identified 11 marine fish taxa at the species level. These taxa belong to 8 families across 8 orders. The predominance of reef-dwelling species suggests that coral reefs play a dominant role as the primary ecosystem in this area. Among the surveyed sites, Barru Waters exhibited the highest species richness (7 species), while Pangkep Waters only revealed 4 species. Through the application of eDNA metabarcoding, this study provided a means to assess fish diversity, delivering crucial foundational information. Our findings highlight the cost-effectiveness of the eDNA metabarcoding method as a powerful scientific tool for the management and conservation of marine fish resources in the Makassar Strait.    
Karakteristik Sampah Plastik di Laguna Segara Anakan Cilacap Nurito, Nurito; Hendrayana, Hendrayana; Husni, Iqbal Ali; Hidayat, Rizqi Rizaldi; Andriyono, Sapto; Rofiq Ulinuha, Mohamad; Hidayati, Nuning Vita
Seminar Nasional Lahan Suboptimal Vol 10, No 1 (2022): Prosiding Seminar Nasional Lahan Suboptimal ke-10 “Revitalisasi Sumber Pangan N
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Nurito N, Andriyono S, Hendrayana H, Husli IA, Hidayat RR, Andriyono S, Ulinuha MR, Hidayati NV. Characteristics of plastic waste in Cilacap segara anakan lagoon. ini. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-10 Tahun 2022, Palembang 27 Oktober 2022. pp. 293-301. Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Plastic litter degrade very slowly in the natural environment. The different characteristics of plastic waste are one of the determining factors in the decomposition process. This study aimed to investigate the type, abundance and characteristics plastic waste in Segara Anakan Lagoon. Plastic waste was taken from Segara Anakan Lagoon on August, 2020 using survey method. Sample was collected from 1x1 meter transect. Analysis of the data used is descriptive analysis. The results showed that we found 7 types of plastics waste, i.e. PET, HDPE, PVC, LDPE, PP, PS, and OTHER. The highest plastic waste abundance was found in station 2 (82,67 items/m2), and the lowest was in station 9 (1,67 items/m2). PP is the dominant type of plastic with a total of 839 items (71.5%) followed by LDPE with a total of 189 items (16.1%), while PVC and PS were found in lowest number, with each of them 6 items (0.5%). The characteristics of plastic waste in Segara Anakan based on the type of plastic waste at station 2 have different types with other stations and the differences are clearly illustrated.
The Effect of Adding Different Amounts of Turmeric (Curcuma domestica) on The Consumer Acceptance Level of Milkfish (Chanos chanos) Amplang aulia, nabila; Andriyono, Sapto; saputra, eka
Journal of Marine and Coastal Science Vol. 12 No. 1 (2023): FEBRUARY
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v12i1.42588

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Turmeric as a natural dye contains curcuminoids which are beneficial for the body and have the potential to be applied in food stuffs such as in amplang. Milkfish amplang (Chanos chanos) is a snack made from typical Indonesian fish which is quite popular in society. This study used experimental laboratory research methods which aimed to determine the effect of adding the amount of turmeric (Curcuma domestica) on the level of consumer acceptance of the chemical and organoleptic characteristics of milkfish amplang. The design used was a completely randomized design (CRD) with one factor, namely the addition of turmeric to the production of milkfish amplang consisting of four treatments and three repetitions. The results showed that there was a significant difference between the treatments (p<0.05), the highest nutritional value was found in milkfish amplang with the addition of 0.5 g of turmeric with an average value of water content (2.64%), ash content (1.75%), protein content (10.54%), fat content (7.51%), and carbohydrate content (77.53%). Organoleptic test results showed that the best treatment was the addition of 0.5 g turmeric with average values for color (8.60), smell (8.40), taste (8.53), and texture (8.80).
Sensory Characteristics and Nutritional Value of Taiyaki with the Addition of Spirulina sp. Powder Eka Nurinsani, Anisa; Sulmartiwi, Laksmi; Andriyono, Sapto
Journal of Marine and Coastal Science Vol. 12 No. 3 (2023): SEPTEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v12i3.44924

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Spirulina is known as a superfood because it is rich in nutrients, both macro and micro. Spirulina has high levels of protein, potassium and polyunsaturated fatty acids (PUFAs) which contain essential amino acids, phycocyanins, essential vitamins and antioxidants. The high nutritional content of Spirulina causes the higher utilization of Spirulina in food products. Taiyaki is a food product originating from Japan and also popular in Korea (bungeo-ppang). The research design consisted of four treatments and five replications. The treatment of adding Spirulina sp powder into the taiyaki filling as much as 1, 2, and 3% of the weight of the filling. The results of the Kruskal Wallis test showed that the addition of Spirulina sp powder had no effect on the parameters of appearance, aroma and texture, on the contrary it had a significant effect on the parameters of taste. The results of the Mann-Whitney follow-up test, the level of preference for taste parameters was not significantly different (p> 0.05) between P0 (without the addition of Spirulina sp powder) and the treatment with the addition of Spirulina sp powder 1% (P1), 2% (P2), and 3% (P3) of the filling weight. The addition of Spirulina sp. in taiyaki products can increase the nutritional value of taiyaki, especially the protein (3.61%) and fat (2.63%) components.
Shelf Life Study of Pedada Fruit Jam (Sonneratia caesolaris) With The Addition of Carrageenan: - Visakhadevi, Jelena; Andriyono, Sapto; Yuli Pujiastuti, Dwi
Journal of Marine and Coastal Science Vol. 13 No. 1 (2024): FEBRUARY
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v13i1.45176

Abstract

Pedada fruit (Sonneratia caseolaris) contains phytochemicals such as steroids, tripenoids and flavonoids. Phytochemical compounds are antioxidants that neutralize free radicals that attack body cells. The application of making pedada fruit jam with the addition of carrageenan can function as a natural preservative. The purpose of this study was to determine the effect of adding carrageenan to the shelf life of pedada fruit jam. The research treatment consisted of 0 week of storage (P0), 1 week (P1), 2 weeks (P2), 3 weeks (P3), and 4 weeks (P4). Parameters observed in this study were the total plate count test, viscosity test, and antioxidant activity test with spektrofotometer Uv-Vis. The data obtained was tested with the ANOVA statistical test and continued with the DMRT test. The results of the research show that antioxidant activity and viscosity have decreasing values with each storage period, while microbiological activity is inversely proportional to antioxidant activity and viscosity, namely with increasing values with each storage period. The storage time for pedada fruit jam with the addition of carrageenan had a significant effect on antioxidant activity, viscosity and microbiological activity which had significantly different values (p < 0.05).
Bioaccumulation of Heavy Metals Lead, Copper, and Zinc in Mangrove Roots Avicennia marina, Water, and Sediment in Panceng Waters, Gresik, Jawa Timur Diyah Retnosari, Fenny; Andriyono, Sapto; Nurmalia Dewi, Nina
Journal of Marine and Coastal Science Vol. 13 No. 2 (2024): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v13i2.52524

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Panceng water is a coastal area that has quite a lot of activities, and this can trigger an increase in waste pollution. This polluting waste can cause an increase in the bioaccumulation of heavy metals in waters. Therefore, this research aims to obtain information on environmental management monitoring activities, such as water quality conditions by determining the concentration of heavy metals Pb, Cu and Zn in the roots of the Avicennia marina mangrove, water and sediment in Panceng Waters, Gresik. The method used was observation, examination of the heavy metals Pb, Cu, and Zn using ICP-MS, and the data was analyzed using quantitative descriptive methods. The results of the analysis of the concentration of the heavy metal Pb in the roots of the mangrove averaged 0.3 mg/kg, Cu 1.205 mg/kg and Zn 0.14 mg/kg. The concentration of heavy metals Pb in sediment was 5.49 mg/kg, Cu 15.7 mg/kg, and Zn 30.6 mg/kg. Meanwhile, the concentration of heavy metals Pb, Cu and Zn in sea water was not detected (ND). Overall water and sediment are still below the threshold. However, the concentration of heavy metals in the roots of the A. marina mangrove is relatively high, above the quality standards of Minister of Environment Decree No. 22 of 2021. Calculation of the level of heavy metal pollution based on Enrichment Factor (EF), Geo-Accmulation Index (Igeo) and Contamination factor (CF) values shows that the heavy metals Pb, Cu and Zn indicate levels of pollution and contamination that are still low to moderate. The Bio Concentration Factor (BCF) of A. marina in Panceng Waters, Gresik is included in the excluder category (BCF<1). The results of this research indicate that the A. marina mangrove can be developed as a phytoremediation agent because it is able to absorb and transfer heavy metals from the environment to other body tissues.
Isolation and Identification of Vibrio parahaemolyticus Bacteria in Bottlenose Dolphins (Tursiop truncates) in Kendal Conservation Pond, Central Java Nafiqoh, Nunak; Setiadi, Setiadi; Novita, Hessy; Lusiastuti, Angela Mariana; Indrawati, Agustin; Elmanaviean; Jannah, Siti Nur; Gardenia, Lila; Penataseputro, Tanjung; Andriyono, Sapto; Ningrum, Siti Gusti; Oktaviani, Dian; Syahidah, Dewi; Wattiheluw, Muhammad Subhan; Purwaningsih, Uni
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.811-819

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Aquatic mammals in Indonesia are officially protected. However, there is a lack of research on these mammals, particularly in relation to potential disease-causing bacteria. A study was conducted in a conservation pond to address this gap, where swab samples were taken from the blowholes of aquatic mammals. The focus was on identifying bacteria that could potentially cause infectious diseases in these animals. The results revealed V. parahaemolyticus bacterial isolates, which showed a 98% similarity to the registered V. parahaemolyticus in NCBI. These bacterial isolates exhibited hemolysin properties and demonstrated resistance to trimethoprim, streptomycin, cephalothin, and penicillin antibiotics.
PELATIHAN IMPLEMENTASI SANITASI DAN HIGIENE PADA PROSES PENGOLAHAN IKAN ASAP KENJERAN Pramono, Heru; Andriyono, Sapto; Wisudyawati, Dita; Sari, Alfi Hermawati Waskita; Hasan, Veryl; Hidayati, Nuning Vita
Balobe: Jurnal Pengabdian Masyarakat Vol 3 No 1 (2024): Balobe: Jurnal Pengabdian Masyarakat
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/balobe.3.1.20-29

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Penerapan higiene dan sanitasi merupakan aspek penting dalam keamanan pangan, termasuk pada pengolahan ikan asap. Kesadaran para pengolah ikan asap akan pentingnya keamanan pangan serta tata cara penerapan higiene dan sanitasi sangat diperlukan. Artikel ini melaporkan proses sosialisasi penerapan higiene dan sanitasi pada pengolahan ikan asap di Kenjeran, Surabaya, Indonesia. Metode pelaksanaan program dimulai dengan brainstorming dengan aparat pemerintah setempat, dilanjutkan dengan pelatihan internasional oleh pembicara Malaysia dan Indonesia, praktik penggunaan peralatan keselamatan, kelas memasak, dan expo. Dari diskusi awal terlihat jelas bahwa masyarakat memahami pentingnya penerapan higiene dan sanitasi, namun masih membutuhkan informasi mengenai peningkatan ukuran pasar (market size). Selama pelatihan internasional, pembicara dari Malaysia dan Indonesia membahas korelasi pemasaran online dan higiene dan sanitasi serta strategi memasak alternatif yang kemudian diimplementasikan pada expo. Produk siap santap (RTE) yang dihasilkan oleh masyarakat potensial untuk dipasarkan lebih lanjut melalui pendekatan online, namun aspek pengemasan dan pemasaran masih perlu dikaji lebih lanjut. Dari penelitian ini, penerapan higiene dan sanitasi di fasilitas pengolahan ikan asap mungkin efektif jika dipadukan dengan cara memasak dan pemasaran alternatif.
KARAKTERISASI KARAGINAN PADA RUMPUT LAUT MERAH (Chondrus crispus) YANG DI EKSTRAKSI MENGGUNAKAN KONSENTRASI KALIUM HIDROKSIDA (KOH) BERBEDA Sapto Andriyono; Naufal Rifqi Pratama; Patmawati Patmawati
Jurnal Perikanan Unram Vol 12 No 2 (2022): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v12i2.286

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Karaginan merupakan polisakarida yang diekstraksi dari beberapa spesies rumput laut merah penghasil karaginan atau karaginofit. Sumber bahan karaginan terbesar saat ini dapat ditemukan pada spesies rumput laut Eucheuma spinosum dan Eucheuma cottoni. Tujuan dari penelitian ini yaitu untuk menganalisis karakteristik karaginan setelah penambahan konsentrasi KOH dan menghasilkan karaginan berdasarkan uji rendemen. Metode eksperimen pada penelitian ini menggunakan Rancangan Acak Lengkap. Perlakuan yang diberikan yaitu pada konsentrasi KOH (10%, 12%, dan 14%). Hasil menunjukkan penambahan konsentrasi KOH dapat meningkatkan rendemen karaginan pada konsentrasi 12%. Penambahan konsentrasi KOH 12% mendapatkan hasil rendemen sebesar 2.43%. Hasil proksimat pada bahan baku rumput laut yang digunakan, yaitu kadar protein 14.68%, lemak 0.4%, karbohidrat 18.8%, air 7.4%, dan abu 46.43%.
STUDI PENAMBAHAN BAHAN PENSTABIL KARAGENAN DALAM PEMBUATAN SIRUP Mangrove ROSELLA Mochamad Alauddin Perdana Putra; Dwitha Nirmala; Sapto Andriyono
Jurnal Perikanan Unram Vol 12 No 3 (2022): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v12i3.349

Abstract

Tanaman mangrove pedada (Sonneratia caseolaris) merupakan komoditas sektor perikanan dan kelautan yang dinilai kaya akan nilai pemanfaatan mulai dari bagian batang hingga buahnya. Adapun keunggulan dari buah ini dapat langsung dimakan serta memiliki berbagai kandungan gizi, vitamin, dan metabolit sekunder. Salah satu diversifikasi produk buah pedada yaitu sirup. Larutan sirup perlu penstabil guna bahan utama mampu terperangkap dalam struktur gel sehingga tidak terjadi endapan serta menghasilkan nilai kekentalan yang tepat. Penelitian ini diharapkan dapat menguji pengaruh karagenan terhadap karakteristik sirup serta nilai kesukaan terhadap kualitas sirup mangrove rosella yang dihasilkan. Sirup mangrove rosella dibuat dari buah mangrove jenis Sonneratia caseolaris dengan ditambah dengan pewarna alami dari kelopak bunga rosella. Metode penelitian yang digunakan yaitu eksperimental dengan Rancangan Acak Lengkap (RAL) dengan pengulangan sebanyak tiga kali pada lima perlakuan yang diterapkan. Perlakuan yang dilakukan yaitu penambahan konsentrasi karagenan diantaranya Kontrol (0%), P1 (0,50%), P2 (0,75%), P3 (1%), dan P4 (1,25%). Parameter utama dalam penelitian ini yaitu gula reduksi, total asam, viskositas dan aktivitas antioksidan sedangkan parameter pendukungnya yaitu uji hedonik. Analisis data menggunakan analisis varians (ANOVA) dengan uji lanjut Beda Nyata Terkecil (BNT) pada taraf tingkat kepercayaan 95%. Hasil sidik ragam menunjukkan penambahan karagenan dengan konsentrasi yang berbeda berpengaruh nyata terhadap gula reduksi, total asam, viskositas, aktivitas antioksidan dan kenampakan tetapi tidak berpengaruh nyata terhadap aroma dan rasa sirup mangrove rosella yang dihasilkan. Berdasarkan uji hedonik, sirup mangrove rosella terbaik pada penambahan karagenan 0,75% yang berpengaruh terhadap rata–rata skor 3,69 yang menunjukkan tingkat kesukaan.
Co-Authors A. Shofy Mubarak Abdillah, Annur Ahadi Achmad Rifky Ananda Adrian Damora Adzhari , Natasya Syafa Aghnina Elvia Fauziah Agung Satriyo Bayu Agustin Indrawati Agustin Sukistyanawati Agustin Sukistyanawati Agustono - Ahasan Habib Ahmad Fahrul Syarif Aini Nurul Ainun Ma’rifa Akhmad Taufiq Mukti Alfi Hermawati Waskita Sari Alifia Rizky Novitasari Alma Ika Fatmawati Alvin Rahardian Alviano Amit Kumer Neogi Andi Aliah Hidayani Andi Aliah Hidayani Andi Aliah Hidayani Andi Parenrengi ANGELA MARIANA LUSIASTUTI Apriliyanto, Mochammad Bayu Asmi Citra Malina Asmi Citra Malina A. R. Tasakka Aulia Dista Mahrunisa Aulia Maulidah Aulia, Nabila Bachruddin, Muhammad Bagus Dwi Hari Setyono Bagus Suseno Boedi Setya Rahardja Catlya Tasya Adella Tasya Adella Chaniful Fuadi, Muhammad Cicik Kurniawati, Cicik Cindi Koes Farizky Crhisnawati Vega Daniel Elkana DARMAWAN SETIA BUDI Daruti Dinda Nindarwi Diamahesa, Wastu Ayu Diniariwisan, Damai Dita Wisudyawati Diyah Retnosari, Fenny Do, Hoang Dang Khoa Dwi Anggorowati Rahayu Dwi Yuli Pujiastuti Dwitha Nirmala Dyah Wahjuning Listyarini Dyahruri Sanjayasari Eka Nurinsani, Anisa Eka Saputra Elmanaviean Endang Dewi Masithah Eva Ayuzar, Eva Fairuzalfa, Najma Fadila Faridha Kusumaningrum Farizky, Cindi Koes Fatimah Fauziah, Aghnina Elvia Firdha Farah Pramesti Firmansyah, Muhammad Ichwan Fitriani, Mirna Gde Raka Angg Kartika Gunanti Mahasri Hanidhan Dwiretno Widiana Hari Pramono Haris, Mochamad Hasanah, Uswa Hastuti, Dewi Wisudyanti Budi Hendrayana Hendrayana, Hendrayana Heru Cahyono Heru Pramono Heru Pramono Hidayat, Rizqi Rizaldi Hidayati, Nuning Vita Hudawi, Mohammad Nuh Husni, Iqbal Ali Hyun-Woo Kim Irfan Wahyudi Islam, Nuruddin Haquel Isroni, Wahyu Kenconojati, Hapsari Lailatul Lutfiyah, Lailatul Laksmi Sulmartiwi Laksono Trisnantoro Larasati, Melani Layli Hamida Lila Gardenia Maharani, Rossa Mahrunisa, Aulia Dista Maradona, Maradona Mardya Syaifudin S Ma’rifa, Ainun Md. Jobaidul Alam Md. Jobaidul Alam Md. Jobaidul Alam Md. Jobaidul Alam Meilina, Sinta Cahya Melani Larasati MIFTAKHUL ULUM Miftakhul Ulum, Miftakhul Mirna Fitrani Mirna Fitrani Mirna Fitrani Mirna Fitrani Mirna Fitriani Mochamad Alauddin Perdana Putra Mochammad Amin Alamsjah Mochammad Bayu Apriliyanto Mohammad Faizal Ulkhaq Moka, Wilma J.C. Mufasirin Muhamad Amin Muhammad Amin Muhammad Chaniful Fuadi Muhammad Hanif Azhar Muhammad Ichwan Firmansyah Muhammad Syahrian Mutiara Rahmawati Mu’alimah Hudatwi N. Juni Triastuti Nafiqoh, Nunak Naufal Rifqi Pratama Nina Nurmalia Dewi Nita Rukminasari Nor Sa'adah Novian Aji Pradana Novita, Hessy Nuning Vita Hidayati Nuning Vita Hidayati Nuning Vita Hidayati Nunuk Dyah Retno Lastuti Nur Indah Sari Arbit Nur Izzati Mohd Noh Nuri Muahiddah Nurito, Nurito Nurmalia Dewi, Nina Nuruddin Haquel Islam Octoriani, Widyanti Oktavia Putri Oktaviana Elsa Dewi Oktaviani, Dian P Kumaladewi Pangestu, Chandra Trisna Panosa, Alief Erwanda Parikesit, Prismadian Aji Patmawati Patmawati Patmawati Patmawati, Patmawati Penataseputro, Tanjung Pipin Suciati Pratama, Naufal Rifqi Pursetyo, Kustiawan tri Putra, Mochamad Alauddin Perdana Putri, Ricke Diana Rachmawati Rusydi, Rachmawati Rahmawati, Ghishella Ayu Rangga Idris Affandi Reza Istiqomatul Hidayah Reza Istiqomatul Hidayah Rikky Leonard Rochma, Shinta Ainur Rofiq Ulinuha, Mohamad Rohsarifuddin, Fadiya Furuujihim Rr. Juni Triastuti Safitri, Diah Ayu Sahidu, Adriana Monica Samara, Syifania Hanifah Saputra, Alfian Rahmadhani Satria Hani Saputra, Hijrah Saroso, Heri Setiadi Setiadi Setyantini, Woro Hastuti Shohifah, Isnatul Umu Siti Gusti Ningrum, Siti Gusti SITI HOTIJAH Siti Nur Jannah Sri Endah Kinasih Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Subekti Suciyono, Suciyono Syahidah, Dewi Syahrian, Muhammad Tatak Dwi Cahyono Tjahjaningsih, Wahju Tjahjaningsih, Wahju Uni Purwaningsih Van Keulen, Mike Veryl Hasan Visakhadevi, Jelena Wahju Thajaningsih Wattiheluw, Muhammad Subhan Widiana, Hanidhan Dwiretno Widyastuti Umar Wijanarko, Puguh Yugo Win Darmanto Woro Hastuti Satyantini Woro Hastuti Satyantini Yuli Pujiastuti, Dwi Yuniawan Heru Santoso Yusuf Bangun Lastianto