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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Teknologi Perikanan dan Kelautan Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) ILMU KELAUTAN: Indonesian Journal of Marine Sciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Ilmu Lingkungan Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan Jurnal Perikanan dan Kelautan Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Journal of Aquatropica Asia Omni-Akuatika LIMNOTEK - Perairan Darat Tropis di Indonesia Jurnal Akuakultur Sungai dan Danau Jurnal Intek Akuakultur Sains Akuakultur Tropis : Indonesian Journal of Tropical Aquaculture Indonesian Aquaculture Journal Jurnal Riset Akuakultur Media Akuakultur Jurnal Kelautan Nasional Jurnal Kebijakan Sosial Ekonomi Kelautan dan Perikanan Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan JURNAL PERIKANAN TROPIS Jurnal Abdi Insani PELAGICUS: Jurnal IPTEK Terapan Perikanan dan Kelautan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Manfish Journal Jurnal Salamata Policy Brief Pertanian, Kelautan, dan Biosains Tropika LIMNOTEK Journal of Marine Studies Jurnal Perikanan JARI : Jurnal Akuakultur Rawa Indonesia
Kukuh Nirmala
Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan Institut Pertanian Bogor, Jl. Raya Dramaga, Bogor, Jawa Barat 16680
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Journal : Media Akuakultur

 1 
FREKUENSI VENTILASI DAN EKSPRESI GEN COI IKAN MAS (Cyprinus carpio) POPULASI SINTETIK KONDISI AMONIA TINGGI Himawan, Yogi; Alimuddin, Alimuddin; Nirmala, Kukuh; Imron, Imron; Suharyanto, Suharyanto
Media Akuakultur Vol 17, No 1 (2022): (Juni, 2022)
Publisher : Politeknik Kelautan dan Perikanan Bone

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/ma.17.1.2022.1-7

Abstract

Ikan mas populasi sintetik dibentuk melalui pencampuran (blending) dari lima strain ikan mas Indonesia, yakni Majalaya, Rajadanu, Sutisna, Wildan, dan Sinyonya.  Ikan mas sintetik potensial untuk dikembangkan menjadi strain tahan ammonia. Penelitian ini bertujuan untuk menganalisis performa ikan mas populasi sintetik sebagai kandidat pembentuk ikan mas tahan amonia tinggi. Evaluasi toleransi terhadap amonia tinggi dilakukan melalui uji tantang NH4Cl sebanyak 200 ppm pada media pengujian benih ikan mas populasi sintetik dan sebagai kontrol ikan mas populasi sintetik tanpa penambahan NH4Cl. Dalam penelitian ini digunakan akuarium dengan ukuran 60x40x40 cm sebanyak tiga unit untuk pengujian frekuensi ventilasi dan dua unit untuk pengambilan sampel. Perlakuan diulang sebanyak 3 kali dengan ikan uji berjumlah 30 ekor tiap akuarium dan bobot 18.32±3.29 g ekor. Pengukuran ekspresi gen CO1 dilakukan dengan metode qPCR. Hasil penelitian menunjukkan frekuensi ventilasi ikan mas sintetik tahan amonia tinggi mencapai 34.00±10.54 bukaan menit-1, lebih tinggi dibanding kelompok pingsan dan kontrol. Tingkat ekspresi gen CO1 ikan mas populasi sintetik yang tahan amonia tinggi memiliki level ekspresi lebih tinggi daripada kelompok pingsan dan kontrol. Sementara itu, kualitas air uji tantang masih berada pada level normal untuk pemeliharaan.A synthetic carp population carp was formed by combining five Indonesian carp strains, namely Majalaya, Rajadanu, Sutisna, Wildan, and Sinyonya. The synthetic carp has the potential to be developed as strains resistant to ammonia. This study aimed to analyze the performance of the synthetic carp population as a candidate to form high ammoniaresistant carp. Evaluation of tolerance to high concentrations of environmental ammonia was carried out by adding 200 ppm NH Cl to  the synthetic carp seed’s rearing media. The control treatment was rearing the seed without the addition of NH 4 Cl. The research used three units of aquarium sized 60x40x40 cm to measure the fish’s gills ventilation rate and two units for sampling. The treatments were repeated three times with 30 test fish per aquarium with an average weight of 18.32 ± 3.29 g per fish. The measurement of CO1 gene expression was performed using the qPCR method. The results showed that the ventilation rate of the synthetic carp with high ammonia resistance was 34.00 ± 10.54 per minute aperture, higher than the unconscious and control groups. The level of CO1 gene 4 expression in the synthetic population of high ammonia-resistant carp was higher than in the unconscious and control groups. In addition, the water quality parameters of the treatment rearing media were within the normal values for carp farming.
Co-Authors . Rasmawan Adianto, Asep Agus Priyadi Agus Waluyo Ahmad Habibie, Ahmad Alexander Burhani Marda, Alexander Burhani Alimuddin Alimuddin Anang Hari Kristanto Ani Widiyati Ani Widiyati Ani Widiyati Ani Widiyati Anwar, Rifky Alwafi Any Widiyati Aras, Annisa Khairani Arfan Afandi Arif Faisal Siburian Arlina Ratnasari Asep Rachmat Pratama Asri, Yuliana Atma Jaya Salman Muin Aziz, Haikal Azril, Muh Berlianti . Berry Juliandi Bianingrum Bianingrum Cecep Kusmana Chandra Yudistira, Chandra Chrismadha, Tjandra D. Djokosetiyanto D. Djokosetyanto Daniel Djokosetiyanto Daniel Djokosetiyanto Daniel Djokosetiyanto DEDI JUSADI Dewi Puspaningsih Dinamella Wahjuningrum Dinar Tri Soelistyowati Dini Islama Dodi Hermawan E. M. Adiwilaga E. Yuniar Eddy Supriyono Enang Haris Enang Harris Enang Harris Enang Harris Enang Harris Surawidjaja, Enang Harris Erlania Erlania Erna Yuniarsih Ernik Yuliana Eva Prasetiyono Evi Susanti Farah Diana farida farida Farida Farida Febrina Amalia Fernando Jongguran Simanjuntak Gunawan, Bambang Kusmayadi Hamim Hamim Hamsiah, , Hamzah, Aris Sando Hanif Azhara, Muhammad Haris luthfi Harton Arfah Hastiadi Hasan Humairani, Humairani Hutapea, John Harianto I Nyoman Radiarta Idil Ardi Ilyas, Anita Prihatini Imam Taufik Iman Rusmana Iman Sari Lubis, Vina Imron Imron Imron Imron, Imron Irzal Effendi Izhar Amirul Haq Jannesa Nasmi John Harianto Hutapea Joni Haryadi Julie Ekasari Kukuh Adiyana Kukuh Adiyana Kuntari, Wahyu Budi Kurnia Faturochman, Kurnia Kurnia Faturrohman Lilis Nurjanah M. Faisol Riza Ghozali M. Zairin Junior Melati, Aulia Firda Mia Setiawati Mochamad Nurdin Moh. Burhanuddin Mahmud Muh Azril Muhamad Yamin Muhammad Amien H Muhammad Firmawan Muhammad Zairin Jr. Munawar Khalil Nafisyah, Eva Nasrul, Muit Novi Susianti Nur Bambang Priyo Utomo Nur Fauziyah Nuradzani, Daffa Nurhidayat Nurhidayat Nurul Taufiqu Rochman Nurul Taufiqu Taufiqu Rochman Prama, Ega Aditya Pras, Eva Prasetiyono Pratama, Asep Rachmat Priyo Handoyo Wicaksono Puji Hastuti, Yuni Purnamawati, , Puspaningsih, Dewi Qorie Astria R. Wulandari Rahmadi Azis, Rahmadi Retnosari Retnosari Revfvi Al Ghaney Rizal Riani Rahmawati RIDWAN AFFANDI Ridwan Affandi Rina Hesti Utami Riri Ezraneti Riri Ezraneti Ris Dewi Novita Riza Purbo Widiasto Rizkiya, Iffi Rizky Armansyah Robin Robin Robin, , Ruku Ratu Borut Ruspindo Syahputra Sabilu, Kadir Sabilu, Murni Saputra, Henry Kasmanhadi Satya, Awalina Setyo Pertiwi Sheny Permatasari Sri Nuryati Sri Nuryati Sri Wahyuni Firman Sudarto Sudarto Sugeng Budiharsono Sugiarti Suhaiba Djai Suharyanto Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sumi Lestari, Fajar Syarif Budiman T. Budiardi Tatag Budiardi Tri Heru Prihadi Tri Heru Prihadi Tri Widiyanto Tridesianti, Siska Triheru Prihadi, Triheru Tyas Setioaji Vika Yuniar Wa Iba, Wa Iba Wahyu Wahyu Wardani, Sulistia WIDANARNI WIDANARNI Widiatmaka Widiatmaka widiatmaka Widiyati, Any Wijianto Wijianto Wijianto Wijianto Wildan Nurusallam Wildan Nurusallam, Wildan Wildan Nurussalam Wirantari, Ayu Puspa Wisriati Lasima Wiwin Ambarwulan Wiyoto Wiyoto Y. Hadiroseyani Yogi Himawan yogi Himawan Yosmaniar Yosmaniar Yosmaniar Yosmaniar Yuli Siti Fatma Yuli Siti Fatma Yuliana Asri Yuni Puji Hastuti Yuni Puji Hastuti Yuni Puji Hastuti