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Rahmat Rasmawan
Chemistry Education Study Programme. Tanjungpura University, Pontianak, West Kalimantan
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Journal : Pharmascience

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Uji Cemaran Mikroorganisme Pada Tiga Varian Daun Kratom (Mitragyna speciosa Korth.) Widian Sari, Margareta Lita; Masriani, Masriani; Warsidah, Warsidah; Yeni, Laili Fitri; Rasmawan, Rahmat
Jurnal Pharmascience Vol 11, No 1 (2024): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v11i1.16230

Abstract

Tanaman kratom (Mitragyna speciosa Korth.) merupakan salah satu komoditas ekspor yang cukup menjanjikan dari Indonesia. Salah satu syarat suatu komoditas dapat diekspor harus memenuhi batasan kandungan mikrobiologi yang mengacu pada SNI dan AHPA. Bahan baku kratom yang terkontaminasi mikroorganisme, terutama mikroorganisme patogen akan membahayakan kesehatan konsumen. Oleh karena itu, penelitian ini bertujuan untuk menganalisis cemaran mikrobiologis tiga varian daun kratom, yaitu kratom merah, putih, dan hijau dalam bentuk serbuk kering simplisia. Uji cemaran mikrobiologi daun kratom meliputi cemaran bakteri Salmonella sp., Escherichia coli, dan kapang khamir. Uji cemaran bakteri Salmonella sp. dilakukan dengan menggunakan media Xylose Lysine Deoxycholate (XLD) dan Brilliat Green Agar (BGA) dengan suhu inkubasi 37oC selama 24 jam. Uji cemaran terhadap bakteri E. coli dilakukan dengan menggunakan media ColiformEscherichia coli Color Indicator dengan suhu inkubasi 35oC selama 24 jam. Penentuan kontaminasi kapang khamir menggunakan media Yeast and Mold Color Indicator dengan suhu 25oC selama 72 jam. Hasil uji yang telah dilakukan menunjukkan bahwa daun kratom merah, putih dan hijau tidak terkontaminasi bakteri Salmonella sp. dan E. Colitetapi terkontaminasi kapang khamir  yang lebih dari 104 koloni/gram. Kata Kunci: Ganja Borneo, Salmonella sp., Escherichia coli, Kapang Khamir, Koloni  The kratom plant (Mitragyna speciosa Korth.) is one of the most promising export commodities from Indonesia. One of the conditions for a commodity to be exported must meet the microbiological content limits referring to SNI and AHPA. Export commodities that do no meet the standards will be detained during the production and delivery process. Therefore, this study aims to analyze the microbiological contamination of three variants of kratom leaves, namely red, white and green kratom. Tests for microbiological contamination of kratom leaves include contamination of Salmonella sp., Escherichia coli, and yeast molds. Salmonella sp. bacteria contamination test carried out using Xylose Lysine Deoxycholate (XLD) and Brilliat Green Agar (BGA) media with an incubation temperature of 37oC for 24 hours. Contamination tests on E. coli bacteria were carried out using Coliform Escherichia coli Color Indicator media with an incubation temperature of 35oC for 24 hours. Determination of yeast contamination using Yeast and Mold Color Indicator media at 25oC for 72 hours. The results of the tests that have been carried out show that the red, white and green kratom leaves are not contaminated with Salmonella sp. and E. coli, but contaminated with yeast and Mold which is more than 104 colonies/gram.
Co-Authors A. Ifriany Harun AA Sudharmawan, AA Abdul Hafiz, Iqbal Adriana Martania Afif Alwanuddin Aghisna, Dewi Agung Hartoyo Agusta, Riko Ahmad Yani T Aisyah Nur Pratiwi Akrom, Akrom Ali Djamhuri Amsal Alhayat Ana Pratiwi, Ana Andi Ifriani Harun Andi Ifriani Harun Andi Ifriany Andi Ifriany Harun Andi Ifriany Harun Andi Rabuandika Anggi Fatmadiwi Anggi Fatmadiwi Anggraeni, Vanny Aninda, Dea Aninindya, Istamarra Aditya Anisa Fitri Annisa Utami Ariadi, Heldi Arifiyanti, Fitria Arina Ulfah Arit Arit Ayni, Ayni Bella Agustina Berliana Berliana Cahyani, Melur Regista Chairul Umam Dea Aninda Dea Prasetya Deden Ariansyah Desi Indra Wahyuni, Desi Indra Desy Ramadhani Putri Dewi, Melyana Dian Fitrianingsih, Dian Dimas Sulistio Dinda Kartika Wijayanti Dwi Widiarti, Dwi Dwi Yunita, Dwi Dzikrika*, Nuuridzdzati Dzul Fadly Egy Hendrawan Elistiani Elistiani Eny Enawati Eny Enawati Eny Enawati Eny Enawati Eny Enawaty Eny Enawaty Erlina ERLINA Erlina . Erlina Erlina Erlina Erlina Erlina Erlina Erni Dwi Astuti, Erni Dwi Ersando Ersando Erwina Oktavianty F02111045, Atikah F02112072, Santy Fadly, Dzul Fajar Suwarno, Fajar Fatmadiwi, Anggi Febiola Pastika Sari Febriyanti, Nita Fenti Paralita, Fenti Fia Tiaraseta Firra Meliana Pratiwiningrum Fitri Apriani Pratiwi fitri pratiwi, fitri Fitri, Sari Nur Fitria Arifiyanti Fitrianti, Nenny Gusti Budjang Hadiwangsa, Dyan Hayuningtrisna Hafiz, Muhammad Iqbal Abdul Hafiza Hafiza hai rida Hairida Hairida Hanifa, Elsa Hapsah, Sri Harun, A. Ifriany Harun, Andi Ifriany Harun, Ifriany Helsa Ulfa Destari Henny Herawaty Hidayat, Aras Husna Amalya Melati Husna Amalya Melati Husna Amalya Melati Ifriani, Andi Ifriany, Andi Iis Puspa Subekti Iis Puspa Subekti Ina Wulandari Indah Nirwana Akay Iqbal Abdul Hafiz Ira Lestari Ira Lestari Ira Lestari Irene Yudea Listra Isniatun Hasanah Isranti Nurul Choiriyah Istamarra Aditya Aninindya Istiqomah Istiqomah istiqomah istiqomah Jenny Pratiwi Johandi, . Khezya Merary Winel Kristiando Siburian L., Maria Merianti Laili Amelia Mardiyatun Laili Fitri Yeni Lastra Lastra Laurelia Lusiana Lestari, Puja Lia Hermawati Listra, Irene Yudea Lukman Hadi Lukman Hadi Lukman Hadi Lukman Hadi, Lukman Lusi, Yohana Mardila Anggela Maria Ulfa Maria Ulfah MARIA ULFAH Maria Ulfah Mariati F17111027, Mariati Marina Ayu Sari Marisa Marisa Marwoto, Putut Maryani Maryani Maryani Maryani Mas riani Masriani . Masriani Masriani Masriani Masriani Masriani, Masriani Mauliyani, Ratih Mawaddatul Warohmah Melur Regista Cahyani Miftahul Kautsari Mitha Yuniaristha Siahaan Monica, Icha Monica, Qois Muhajjir, Ahmad Ridho Muhammad Iqbal Abdul Hafiz Muharamiah, Tenti Muharini, Rini Mulida, Mulida Mutiara, Syrifah Nur Intan Nanik Yuningsih Natasya, Qois Neni Haryani Nggua Li, Maria Yersiana Nina Mardiana (F01108057) Nisa Istiqomah Harahap, Nisa Istiqomah Nizam, Dinna Nina Mohd Noer Hidayatullah Novi Dwi Trisnawati Nugroho, Sunyoto Eko Nunung Nurjannah, Nunung Nur Ainun Nur Riska Safitri Nurhafizah Nurhafizah Nurhasnah F02111048, Nurhasnah Nurlianti Nurlianti, Nurlianti Nurmala Nurmala Nurul Hasanah Nurul Salima Nyemas Hidayati Patya Mudira Mufti Akbar Pingkan Ramadhan Sailendra Pingkan Ramadhan Sailendra Pinta Rini Harpiyani Pitra Dewi Pranata, Rio Pratami, Hutianas Muhar Pratiwi, Divya Indah Prayuda, Riandi Purba, Nasriani Purwanto, Sandi Qois Natasya Rachma Julianty Putri Rachma Julianty Putri Rachmat Sahputra Rachmat Sahputra Rachmat Sahputra Rachmat Sahputra Rachmat Sahputra Rachmat Sahputra Rachmat Saputra Rachmawati, Fitriyani Ikka Rahma, Saparina Ramadhan Sailendra, Pingkan Ramadhania, Dewi Yona Renaningtyas, Agneviana Pratikta Ditya Rengen, Yunita Ellysa Resha Meisya Ariana Rico Hediyansah Rini Muharini Rini Muharini Risya Sasri Rody Putra Sartika Rusfamahira Rusfamahira Rusnani Rusnani Sabdaningtyas, Katherina Sahputra, Rachmad Sapar, Ajuk Sari, Widyan Sasri, Risya Sawe, Agustina Sekunda Segarti, Alvia Seilendra, Pingkan Ramadhan Selina, Selina Septiana, Tri Merry Setya Wardani, Yulia Wahyu Sholehat, Muhammad SRI RAHAYU Suliono Suliono Suprianto, Bintang Sutamah, Sutamah Syamswisna , Syuhaif Al Af Syah TATI NURHAYATI Tiya Ruslina Putri Tomo Djudin Tomo Djudin Tri Indri Yani Trisnawati, Resty Tulus Junanto Tulus Junanto Tulus Junanto Tulus Junanto Tulus Junanto Tulus Junanto Ubadilla Mairani Ulfa, Nadia Utami, Nurzam Indah Utin Thiya Novasari, Utin Thiya Valentina Winda Permata Wage Isnadini Wahdah Aliyah Waliyah, Isrul Warohmah, Mawaddatul Warsidah Warsidah Warsidah Warsidah Warsidah, Warsidah Widian Sari, Margareta Lita Widyan Sari Winel, Khezya Merary Wiwin Winarsih yarso, Pratiwi Yesy Palma Yuli Yanti Yuliarita, Yuliarita Yuniarti Yuniarti Yunita Puspasari Yunita Puspasari