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POTENSI NETRALISASI IMUNOGLOBULIN Y ANTITETANUS YANG DIISOLASI DARI TELUR AYAM (THE POTENCY NETRALIZATION OF ANTI TETANUS IMMUNOGLOBULIN Y THAT WERE ISOLATED FROM CHICKEN EGGS) I Nyoman Suartha; I Wayan Teguh Wibawan; Retno Damayanti Soejoedono; Bibiana W. Lay
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The porpuse of study was to explore the potential use of? anti tetanus IgY from eggs yolk as a substitute for anti tetanus serum raised in ?horses. The eggs were collected from chickens which have previously been immunized with tetanus toxoid. Neutralization potency test of anti tetanus IgY determined by ?Spearman-Karber method.? The highest mean titer of anti tetanus of egg yolk was 80.16 ? 33.55 IU/ml and the lowest was 1.69 ? 0.63 IU/ml. The concentration? of purified IgY was 1.644 ? 0.424 mg/ml. Spearman-Karber value of potency of anti tetanus IgY are 35 IU/ml. ?This research concluded that Chickens was capable of produced of anti tetanus in eggs yolk with value of potency are 35 IU/ml.
Laporan Kasus: Obstruksi Rektum dan Uretra Akibat Fibroma Serviks pada Anjing Golden Retriever (RECTAL AND URETRA OBSTRUCTION DUE TO FIBROMA CERVICES IN GOLDEN RETRIEVER DOG: CASE REPORT ) I Nyoman Suartha; I Wayan Wirata; I Wayan Gorda; Agatha Serena Lumban Tobing; I Ketut Eli Supartika
Jurnal Veteriner Vol 21 No 1 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Rectal obstruction in dogs is generally caused by foreign objects such as bone or hard feces, whereas urethral obstruction is generally caused by calculi. Cases of rectal and urethral obstruction due to tumors or neoplasia in the female reproductive tract of dogs are rare. Fibroma is one type of benign tumor that attacks fibroblast tissue. The formation of the tumor results in obstruction of the gastrointestinal tract and urine. This paper aims to raise cases of rectum obstruction and vesica urinaria in dogs. Fourth yearold female golden retriever dogs was admitted to Veterinary Hospital Faculty of Veterinary Medicine Udayana University. According to the case history of owner, the dog was suffered weakness, anoreksia, bally and tend. The dog was difficult to urinate and poo. The stool was suppressed like “ornament cake birth day”. All of health condition were normal. Clinical examination revealed presence mass like tumor attached to the cervix with consistence hard and irregular form. Ultrasonography examination was distended bladder without calculi form. Hematological routine have leucophilia, anemia and thrombositopenia. After general anesthesia with ketamine HCl and xylazine, the mass with irregular form and hard consistence was surgical removed. Histophatological section were revealed presence of fibroma which represented by arrangement of cell that have feature tapering cell as fibroblast without mitosis. The mass was diagnosed as fibroma. It concluded that golden retriever dogs had obstruction of rectum and vesica urinaria as result of cervical fibroma tumors.
Gambaran Histopatologi Kulit Anjing Penderita Dermatitis (HISTOPATHOLOGICAL FEATURES OF DOG’S SKIN WITH DERMATITIS) Komang Andika Purnama; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Gusti Made Krisna Erawan; I Made Kardena; I Nyoman Suartha
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Complex dermatitis is a disorder of the skin caused by the combination of several agents such as fungi, ectoparasites, bacteria, and metabolic diseases with combined clinical symptoms of primary lesions and secondary lesions. This study aims to determine the histopathology of dog skin with complex dermatitis. There were 15 samples of dogs with complex dermatitis, skin biopsy and histopathology. The results showed that the incidence of complex dermatitis in young dogs was 60%, long-haired 67%, male sex 73%, and domestic race 73%. Macroscopic examination found a change in hyperkeratosis. Microscopically observed that infiltration of polymorphonuclear cells, monomorphonuclear cells and macrofag from moderate to severe in the dermis. In hair follicles and epidermis occured akantosis, and keratin proliferation. There were segment fragments from infectious agents such as Demodex canis, Sarcoptes scabiei, and yeast from Malasezia sp. Other changes were observed in the presence of hydrophic degeneration, necrosis, and ulcers. Dogs suffering from complex dermatitis have chronic infections with more than one infectious agent. Disabilities based on histopathological observation of dogs with complex dermatitis with moderate severity of 33%, and severe 67%.
Kepekaan Telur Spesific Pathogen Free dan Clean Egg Terhadap Virus Flu Burung (SENSITIVITY OF SPESIFIC PATHOGEN FREE EGGS AND CLEAN EGG TO THE AVIAN INFLUENZA VIRUSES SUBTYPE H5N1) Gusti Ayu Yuniati Kencana; I Nyoman Suartha; Arini Nurhandayani; Muh Ramadhan
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Avian Influenza which is  in Indonesia  known as Flu Burung  is caused by the avian influenza virussubtype H5N1 (AIV-H5N1). Vaccination is one of the major strategies for preventing and eradicatingAIV-H5N1 in Indonesia. Several factors can affect the potential vaccine such as viral content and mediaused for the propagation of the virus. One of the media commonly used to propagate  the virus is pathogenspecific free (SPF) embryonated chicken eggs. However, as the SPF eggs production is limited and expensive,the use of clean embryonated chicken eggs as an alternative need to examined. This study aimed todetermine the sensitivity of SPF and clean embryonated chicken eggs to the AIV-H5N1. The virus usedwas seed avian influenza virus (A/ Chicken/West Java (Subang)/29/2007)  which haa previously werepropagated  in SPF eggs and the Clean Eggs. The virus titer was determined as Embryo infective Dose 50%(EID50) using Reed and Muench method. Sensitivity of SPF eggs and Clean Egg to the VAI-H5N1 wascompared using  Chi-square statistical analysis. The titers of Avian Influenza Virus subtype H5N1 were106.83EID50/0.1ml in SPF eggs and 106.17EID50/0.1 ml in the Clean Eggs. Statistical analysis showed that,the sensitivity of SPF Eggs and Egg Clean  for the propagation of the VAI-H5N1 was not significantlydifferent.
Peranan Pedagang Unggas dalam Penyebaran Virus Avian Influenza I Nyoman Suartha; I Made Suma Antara; I Kadek Saka Wiryana; I Made Sukada; I Wayan Wirata; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 4 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

A questionnaire surveillence have been carried out in three different traditional markets (ie. Beringkitin badung district, Kumbasari in Denpasar, Kediri in Tabanan district) in order to understand the role ofpaultry traders behavior in transmitting of avian influenza virus. Of 150 quationares collected most oftraders (66.7%) kept the animals for 1-3 days before it was marketed. Traders bin Beringkit and Kediri(76.3%) used to mix different species of birds in their cages, whereas none of the traders from Kumbasaridoing that. When hygienec and sanitation aspects were considered (ie. Washing and desinfectan sprayingfor cages) it was found that the behavior of traders varied markedly between the 3 different market. Inconclusion the traders awareness to especially bird flue infection and implementation of biosecurity isvery low.
Deteksi Virus Classical Swine Fever di Bali dengan RT-PCR I Wayan Wirata; Ida Ayu Sri Chandra Dewi; I Gusti Ngurah Narendra Putra1,; Ida Bagus Oka Winaya; Ida Bagus Kade Suardana3,; Tri Komala Sari; I Nyoman Suartha; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Classical Swine Fever (CSF) virus has been confirmed for the first time in pig in Bali. The object of thisstudy was suspected CSF cases diagnosed at the diagnostic laboratory assistantship of the Faculty ofVeterinary Medicine, Udayana University, in 2007-2008. Total number of cases was 12. Case recordsincluded the signalment of case (breed, age, body weight, and the origin of respective case), clinical signs,post-mortem lesions, and histological pictures. CSF virus was confirmed using the standardized reversetranscriptase-polymerase chain reaction (RT-PCR) for CSF from European Union. One RT-PCR productwas sequenced. CSF virus was confirmed in seven out of 12 cases (58%). The cDNA sequence wasconfirmed to be specific of CSF E2 protein coding region with 98% homology to one isolate from China thatwas available in GeneBank. Further works are recommended to elucidate the sensitivity of RT-PCR, toclarify some differential diagnose, and to find out the genetic variation of CSF virus in Bali.Key words: classical swine fever virus, Bali, RT-PCR
Pembiusan Monyet Ekor Panjang (Macaca fascicularis) dengan Campuran Ketamine dan Xylazine (THE ANAESTHETIZATION OF LONG TAILED MACAQUE (MACACA FASCICULARIS) BY INJECTING THE COMBINATION OF KETAMINE AND XYLAZINE) I Nyoman Suartha; I Nengah Wandia; I Gusti Agung Arta Putra; I Gede Soma; I Gusti Ngurah Sudisma
Jurnal Veteriner Vol 2 No 1 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Pembiusan Monyet Ekor Panjang (Macaca fascicularis) dengan Campuran Ketamine dan Xylazine   (THE ANAESTHETIZATION OF LONG TAILED MACAQUE (MACACA FASCICULARIS) BY INJECTING THE COMBINATION OF KETAMINE AND XYLAZINE)
PERBANDINGAN ANTARA METODE PEG-AMONIUM SULFAT DAN PEG-KLOROFORM UNTUK EKSTRAKSI DAN PURIFIKASI IGY KUNING TELUR I Nyoman Suartha; I Wayan Teguh Wibawan; Ratu Shinta Mayasari
Jurnal Veteriner Vol 7 No 4 (2006)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Metode ekstraksi protein kuning telur dengan polyethylene glycol (PEG) yang dikombinasi dengan ammonium sulfat
Vaksin Polivalen Untuk Mencegah Penyakit Flu Burung (POLIVALEN VACCINE TO PREVENT BIRD FLU DISEASES) I Nyoman Suartha; I Wayan Wirata; I Gusti Ngurah Narendra Putra; Ni Made Ritha Krisna Dewi; I Made Suma Anthara; I Wayan Teguh Wibawan; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study was carried out to determine the use of bird flu polyvalent vaccines containing two or threeor more virus isolates representating of circulating viruses in the region. Three seed isolates of avianinfluenza H5N1 virus were used in this experiment. The isolates were Chicken/Denpasar/Unud-01/2004,Chicken/Klungkung/Unud-12/2006, and Chicken/Jembrana/Unud-17/2006. The seeds were inactivatedusing 0.01% formaldehide than mixed (AI3G) alumunium hidroxide adjuvant and then injectedintramuscularly to Isa Brown layer chicken at 3 weeks of age and repeated at the age of 5 weeks. The doseof each seed virus was 27 HA units. Sera were collected at one and two weeks after the second vaccination.The result showed that the arithmetic meant titer (AMT) of sera that tested with homologous isolate washigher than the test using a heterologous isolates, in the standard haemaglutination inhibition (HI) assay.The mixed AI3G vaccine produced a uniform AMT against the constituent isolates, while vaccines withindividual isolate yielded a lower and more variation in AMT. Further experiments using a commercialhomologous H5N1 and heterologous H5N2 commercial vaccines has resulted AMT that 1-4 log lower thanAI3G vaccine. It is concluded that polyvalent vaccine with field seed isolates is recommended to be appliedin the poultry farm in Indonesia.
Survei Penyakit Porcine Reproductive and Respiratory Syndrome pada Peternakan Babi di Bali (SEROLOGICAL SURVEILLANCE OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME IN PIGGERIES IN BALI) I Nyoman Suartha; I Made Suma Anthara; I Wayan Wirata; Tri Komala Sari; Ni Made Ritha Krisna Dewi; I Gusti Ngurah Narendra; I Gusti Ngurah Mahardika
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aimed to determine the presence and burden of Porcine Respiration and ReproductiveSyndrome (PRRS) virus in pig farms in Bali. A total of 305 sera samples were collected from 10 intensivepig farms and backyard piggeries located in eight districts, in Bali. The PRRS antibody and the virus wasdetected using enzyme linked immunosorbent assay (ELISA) and transverse reverse polymerase chainreaction (RT-PCR), respectively. The results showed that, generally the average percentage of positiveswine anti-PRRS antibody was 13.4%, 14.3%,  and 11.7% in the backyard farms and commercial farms,respectively. Whereas, the detection rate of PRRS virus was 8.9% (15.3% and 5.6% in the backyard farmand commercial farms, respectively). It was concluded that PRRS virus is endemic in pigs, in Bali.Vaccination, management, biosafety, and quarantine  should be implemented to prevent the economicloss due to PRRS.
Co-Authors A A N G D Wisesa A A N O Pujawan A A S I Pradnyantari Abram Hary Batistuta Adi Setiawan Adnyana, Ida Bagus Nararya Primastana Agatha Seren Lumban Tobing Agatha Serena Lumban Tobing Aida Lousie Tenden Rompis Alya Nita Shena Gayanti Anak Agung Ayu Mirah Adi Anak Agung Gde Oka Dharmayudha Anak Agung Sagung Istri Pradnyantari Anak Agung Sagung Kendran Anak Agung Sagung Kendran Ananta, Muhammad Ghufron angelina serlin Anita Dwi Handayani Annisa Budiani Annisa Putri Cahyani Annisa Putri Cahyani Apriliana, Kadek Soma Arini Nur Handayani Arini Nur Handayani Arini Nurhandayani Arini Nurhandayani Ayu Fitriani Baiq Indah Pertiwi Bambang Pontjo Priosoeryanto Bhakty, Zatya Wira Bhaskara, Audrey Febiannya Putri BIBIANA W LAY Boro, Saptarima Eka Estiani Cahyaniarta, I Kade Candra Cyrilus Jefferson Bour Daniel Raja Bonar Nainggolan Dewa Odiec Purnawan Dewanti, Desak Gede Bintang Pradnya Dewi, Desak Made Wiga Puspita Dewi, Putu Ayu Purbani Novia Diana Mustikawati Duarsa, Bima Satya Agung DWI SURYANTO Dyah Utami Dewi EKA MAHARDHIKA RATUNDIMA Emy Sapta Budiari Eustokia Yulisa Madu, Eustokia Yulisa Fajar Mubarok Fatmawati Aras Fernandes, Nuno G.A.M.K. Dewi Gede Herdian Permana Putra Gusti Ayu Kencana Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yunianti Kencana Gusti Ayu Yuniati Kencana Heparandita, Ananda Agung Dextra I Gede Soma I Gede Soma I Gusti Agung Arta Putra I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Gusti Made Krisna Erawan I Gusti Ngurah Badiwangsa Temaja I Gusti Ngurah Bagus Trilaksana I GUSTI NGURAH DIBYA PRASETYA I GUSTI NGURAH KADE MAHARDHIKA I Gusti Ngurah Kade Mahardika I Gusti Ngurah Mahardika I Gusti Ngurah Mahardika I Gusti Ngurah Narendra I Gusti Ngurah Narendra Putra I Gusti Ngurah Narendra Putra I Gusti Ngurah Narendra Putra I Gusti Ngurah Narendra Putra1, I Gusti Ngurah Narendra, I Gusti Ngurah I Gusti Ngurah Sudisma I GustiKetut Suarjana I K. SUATHA I KADEK SAKA WIRYANA I Ketut Eli Supartika I Ketut Gunata I Ketut Gunatha I Ketut Suada I Ketut Tomy Caesar Ramanda I Komang Wahyu Yuliana I Made Dira Swantara I Made Kardena I Made Merdana I Made Sukada I MADE SUMA ANTARA I Made Suma Anthara I Nengah Sudarmayasa i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Windhu Paramarta I Putu Gede Yudhi Arjentinia I Putu Indra Parmayoga I Putu Sudiarta I Putu Wira Adi Wibawa I W Y Semarariana I Wayan Batan I Wayan Bebas I Wayan Gorda I Wayan Suardana I wayan Teguh Wibawan I Wayan Wirata I. B. K. Suardana I.A.M.L. Dewi I.A.P. Apsari I.A.P. Gayatri I.B.K. Suardana I.G.A.A. Idayati I.H. Utama I.W. Batan Ida Ayu Dian Kusuma Dewi Ida Ayu Pasti Apsari Ida Ayu Sri Candra Dewi Ida Ayu Sri Chandra Dewi Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Iman Bayu Prakoso Darmono Insani, Widia Iwan Harjono Utama Kadek Karang Agustina Kamaliana, Baiq Reni Ketut Budiasa Ketut Sepdyana Kartini Kevin Dominika Komang Andika Purnama Kristiana Yoaltiva Jinorati Kristina Kristina Ledi Natalia Surbakti Levina, Stephanie Luh Dewi Anggreni Luh Gde Sri Surya Heryani Luh Kadek Nanda Laksmi Luh Made Sudimantini Luh Made Sudimartini Lusiana Lasmari Siahaan Luwis, Jeremy Christian M P A Yunikawati M Windhu M.D. Rudyanto Madania, Reydanisa Noor MADE KOTA BUDIASA Made Ririn Sri Wulandari Made Suma Anthara Made Suma Anthara Margaretha Dhea Sinthalarosa Marmanto, Tessa Saputri Mawar Datu Allo Dendang Megariyanthi, Ni Putu Arie Melkias Oagay Melkias Oagay Muh Ramadhan Muhammad Ulqiya Syukron Musdalifa, Annisa Nareswari, Ayu Widya Nazara, Nonitema Nengah Tegar Saputra Ni Ketut Dias Nursanty Ni Ketut Suwiti Ni Ketut Suwiti Ni Komang Ade Juliantari Ni Luh Eka Setiasih Ni Luh Putu Dharmawati Ni Luh Putu Kusuma Clara Dewinda Ni Luh Putu Sriyani Ni Luh Putu Yunita Listiana Dewi Ni Luh Watiniasih Ni Made Ayu Sintya Paramita Ni Made Ernawati Ni Made Restiati Ni Made Rita Krisna Dewi Ni Made Ritha Krisna Dewi Ni Made Ritha Krisna Dewi Ni Made Ritha Krisna Dewi Ni Made Ritha Krisna Dewi2 Ni Nyoman Werdi Susari Ni Nyoman Werdi Susari Ni Putu Ayu Dewi Wijayanti Ni Putu Tiara Indriana Ni Wayan Tatik Inggriati Norawigaswari, Nengah Desy Nyoman Sadra Dharmawan P S Dwipartha P T E Sucitrayani P.I.S.S. Oka P.K. Pebyanthi Patabang, Denselina Lilis Prasatya, I Gde Made Abdi Priska Mariane Serang Putrawan, Baja Sadhayu Putri Utami Putri, Ayu Chitra Adhitya Putu Adi Cahya Dewi Putu Adrian Junaedi Putu Ayu Sisyawati Putriningsih Putu Devi Jayanti Putu Gede Yudhi Arjentinia Putu Hendrywaesa Sudipa Putu Henrywaesa Sudipa Putu Henrywaesa Sudipa Putu Henrywaesa Sudipa Putu Henrywaesa Sudipa Rahim, M. Andry Ratu Shinta Mayasari Remontara, Al Afuw Niha Reny Septyawati Retno Damayanti Soejoedono Rui Daniel de Carvalho S.K. Widyastuti Sachio, Drevani Angelika Samosir, Hartina Saputra, I Nyoman Dwi Eka Saweng, Cikal Farah Irian Jati Sawitajaya, I Made Sayu Raka Padma Wulan Sari, Sayu Raka Padma Wulan Septianingsih, Ni Luh Putu Diah Sheira Tannia Welfalini Sibarani, Oktryna Hodesi Sitohang, Martina Tiodora Situmorang, Fernando Jose Immanuel Clinton Sri Kayati Widyastuti Sri Kayati Widyastuti Sry Agustina Suarniti, Ni Luh Putu Sukoco, Hendro Sutadisastra, Nisha Aisya Suwartini, Ni Komang Swandewi, Ni Kadek Meita Syamsidar Syamsidar Syarif Lalu Hidayatullah T. Sari Nindia Tahlia, Ninis Arsyi Taruklinggi, Utari Resky Tiara L Rona Tjok Gde Oka Pemayun Tjokorda Sari Nindhia TRI KOMALA SARI Tri Suci Galingging, Tri Suci Valerie Xylia Tay Vivi Indrawati Widyanti, Agnes Indah Wijaya, Dhyana Ayu Manggala Willy Moris Nainggolan Wirawan, I Gede WIWIK SUSANAH RITA Wulandari Wulandari Yekhonya Rehuel Sidjabat Yoana Pratiwi Clara Pakpahan Yoga Pratama Nuradi Yosaphat L.S Kote Zumara Mufida Hidayati