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Kintoko Kintoko
Prodi Magister Pendidikan Matematika, Pascasarjana, FKIP – UNS
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Journal : Syntax Literate: Jurnal Ilmiah Indonesia

 1 
Pengaruh Fermentasi Laktat terhadap Kandungan Fenolik Total dan Aktivitas Antioksidan Poliherbal TBR serta Uji Profil Lipid secara in Vivo Mutti, Annisa Aulia; Kintoko, Kintoko; Sulistyani, Nanik
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v10i3.58198

Abstract

Lactate fermentation, such as yogurt, is known to increase antioxidant activity. In addition to yogurt, fermentation can also be applied to herbal plants. Polyherbal plants Taxus sumatrana (Sumatran cypress), jixueteng (Spatholobus suberectus), and pearl grass (Hedyotis corymbosa) (TBR) contain total phenolic compounds that act as antioxidants and can lower cholesterol levels. This study aims to analyze the effect of TBR polyherbal fermentation on total phenol content and antioxidant activity. Polyherbal extraction is carried out by the maceration method using 96% ethanol for 24 hours. Total phenolic tests were carried out using gallic acid, followed by aerobic fermentation with lactic acid bacteria Streptococcus thermophilus and Lactobacillus bulgaricus with 1%, 2%, and 3% TBR extract concentrations. Antioxidant activity was measured by the DPPH method, and lipid profile tests were performed in vivo on 35 white rats (Rattus norvegicus), which were divided into 7 treatment groups. The results showed that TBR polyherbal fermentation significantly increased the total phenolic levels, with a concentration of 3% resulting in the highest levels (41.689 ± 0.5682% GAE). Although antioxidant activity decreased slightly after fermentation, it remained in the strong category (IC50 76.816–88.283 μg/mL). In vivo tests showed that polyherbal yogurt TBR 3% lowered triglyceride and total cholesterol levels and slightly increased HDL levels, but was less effective in lowering LDL. In conclusion, TBR polyherbal fermentation increases total phenolic levels while maintaining strong antioxidant activity, with potential as a natural source of antioxidants for lipid profile improvement.
Fermentasi Poliherbal TBR Terhadap Kandungan Flavonoid Total dan Aktivitas Antioksidan serta Berat Badan Secara In Vivo Atmarani, Rizki Essa; Kintoko, Kintoko; Sulistyani , Nanik; Taufik, Epi
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v10i3.57968

Abstract

Minuman fermentasi diketahui memiliki efek yang dapat meningkatkan kadar flavonoid total dan antioksidan,serta dapat mempengaruhi berat badan yang disebabkan oleh reaksi dari bakteri asam laktat. Antioksidan pada tanaman, Taxus Sumatrana (cemara sumatra), bajakah jixueteng (spatholobus suberectus) dan rumput mutiara (Hedyotis corymbosa) dilaporkan memiliki kandungan flavonoid total dan kadar antioksidan tinggi. Penelitian ini bertujuan untuk membuat sediaan minuman fermentasi poliherbal,serta mengukur kadar flavonoid total dan antioksidan Yoghurt Polihrerbal TBR (3:1:1) serta mengetahui pengaruhnya terhdap penurunan berat badan dan indeks organ hewan uji. Metode ekstraksi yang digunakan dalam penelitian ini adalah metode maserasi dengan menggunakan pelarut etanol 96% selama 1x24 jam, selanjutnya dilakukan pengujian flavonoid total menggunakan baku standar kuersetin, setelah itu difrementasi menggunakan Bakteri Asam Laktat (BAL) dengan konsentrasi ekstrak TBR (3:1:1) 1%, 2% dan 3% , lalu pengujian antioksidan dengan metode ABTS serta pengukuran berat badan dan indeks organ pada tikus putih (Rattus novergicus) dengan 7 kelompok perlakuan berbeda. Analisis data penelitian ini menggunakan data komputasi dan statistik menggunakan softwere SPSS VER 2.0.
Co-Authors Akrom, Akrom Alfarisyi, Muhammad Iqbal Ananda, Aghnia Nabila Anggraeni, Linda Suci Ari Satia Nugraha Aris Setyawan Ariyanti, Lusi Mardika Astuti, Andarweni Atmarani, Rizki Essa aviory, koryna Ayu Shabrina Billy Harnaldo Putra, Dini Fatmi, Botutihe, Lisa Agustina Cahayani, Devita Mika Cendekiawan, Khrisna Dani Kusuma Desmayanti, Astri Dhian Arista Istikomah Dian Aprilia, Dian Dona, Suzana Dwi Utami Eka Siswanto Syamsul Epi Taufik Faisal Nur Arib Fajar Fajar Farid , Muhammad Ferani Mulianingsih Gunawan Gunawan Gunawan, Adhi Gusriyani, Sri Gustinanda, Rizky Hadi Siswanto, Deny Hardi Astuti Witasari Hari Susanti Harry Ade Saputra Hasana, Siti Nur Hutari, Andri Iis Wahyuningsih Ilmia, Nisrien Indahsari, Kharisma Safitri Nur Jana, Padrul Khrisna Agung Cendekiawan Khusnul, Latifah Kusumo, Djati Wulan Lestariningrum, Wahyu Titis M. Yogi Riyantama Isjoni Maghfirah, Yolanda Sajjida Makati, Annisa Camellia Mehta, Kunal Meki Pranata Mochammad Saiful, Bachri Muhammad Iqbal Birsyada Muldiyana, Tya Mustofa Ahda Mutti, Annisa Aulia Nanik Sulistyani Nina Widyaningsih, Nina Ningrum, Lailatul Fitria Nining Sugihartini Novitasari, Putri Rachma Nur Arib, Faisal Nurkhasanah Mahfudh Nurlita, Tasya Nuryani, Eva Oktavia, Lisa Pamungkas, Megita Dwi Poerwahyuningrum, Neny Pulhehe, Hasni Rangkuti, Ahmad Faizal Ranti, Imaniar Rejeki, Marsudi Endang Sri Restu Ayu Mumpuni, Restu Ayu Ridho, Anisa Rifka Rifki Febriansah Rohma, Siti Rohmah, Lely Arbiati Nur Rukaya, Benazir Evita Sa’adah, Hayatus Safira, Rizka Santoso, Blegoh Iwan Sapto Yuliani Saputri, Ginanjar Zukhruf Septian Aji Permana Septiana Wijayanti Setianingrum, Ervina Setiyawati, Fitri Indah Shary, Aprilya Kumala Siswanto, Deny Hadi Sri Budiarti, Nur Idha Suhendra Suhendra Sulistyani , Nanik Sunanti, Titis Supomo Supomo Suryaningsih, Andri Susetyawati, MM Endang Susetyawati, MM. Endang Susetyowati, MM Endang Tri Puspita Yuliana Urmatul Waznah Utami, Febri Via Yustitia Wahyu Widyaningsih Wahyuni, Dina Wardhani, Firda Aprillia Wardhani, Firdha Aprillia Warniasih, Kristina Widyaningrum, Rachmawati Wuryandari, Tutik Wuryandari, Tutik Yanti, Asti Afrida Yuli Nurullaili Efendi, Yuli Nurullaili Yulia Yesti, Yulia Yulianto Yulianto Yuni Suprapto, Yuni