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All Journal Jurnal Sain Veteriner Jurnal Veteriner Indonesian Journal of Tropical and Infectious Disease Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Journal of Fisheries & Marine Jurnal Agro Veteriner

Setiawan Koesdarto

Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 515120

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Anthelmintic Activity of Basil Leaves (Ocimum sanctum Linn.) Infusion Against Ascaris suum In Vitro Ogen Sea; Mas'ud Hariadi; Setiawan Koesdarto; Kusnoto Kusnoto; Ngakan Made Rai Widjaja
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Ascaris suum is a parasite nematode that causes infection in swines with high prevalence rates in host populations and usually associated with liver damages called â€œmilk spotsâ€ caused by larvae migration, resulting in organ condemnation. Basil leaves (Ocimum sanctum) phytochemical constituents contains flavonoid, phenol and tannin. Tannins and phenolics are known to interfere with the energy generation in helminth parasites by uncoupling oxidative phosphorylation and also bind to free proteins in the gastrointestinal tract of host animal or glycoprotein on the cuticle of the parasite and leading to death. This study was aimed to determine the activity of basil leaves (Ocimum sanctum) infusion in several concentrations against A. suum in vitro. This research used six treatments and four replications. This research used 10 A. suum in each treatment with four replication. The observations were done at 12, 18, 24, 30 and 36 hours in an incubator at 37oC. Based on the data analysis, basil leaves (Ocimum sanctum) infusion has anthelmintic activity against A. suum in vitro. The greater of the concentration and the longer of time of immersion, will make the death percentage of A. suum become higher. Concentration of basil leaves (Ocimum sanctum) infusion 15% is the effective concentration that can kill 100% of the A.suum during 36 hours of immersion.

The Anthelmintic Activity of Ethanol Extract of Basil Leaves (Ocimum americanum Linn.) Againt Haemonchus contortus Death in Vitro Rari Noviana; Chairul Anwar Nidom; Agus Sunarso; Setiawan Koesdarto; Sri Mumpuni Sosiawati; Iwan Sahrial Hamid
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

Tujuan dari penelitian ini adalah untuk mengetahui daya anthelmintik dari ekstrak etanol daun kemangi (Ocimum americanum Linn.) terhadap mortalitas cacing Haemonchus contortus secara in vitro. Sediaan ekstrak dibuat dengan konsentrasi (berat/volume) 2,5%, 5%, 7,5%, dan 10%. Untuk kontrol positif digunakan PBS dan DMSO sedangkan untuk kontrol negatif digunakan Albendazole 10 mg/ml. Sebanyak 10 ekor cacing Haemonchus contortus betina direndam dengan berbagai perlakuan dalam cawan petri. Masing-masing perlakuan diulang sebanyak 4 kali. Pengamatan dilakukan setiap 1 jam sekali selama 6 jam untuk melihat apakah cacing masih hidup atau sudah mati. Hasil dari penelitian ini menunjukkan bahwa ekstrak daun kemangi memiliki efek anthelmintik terhadap cacing Haemonchus contortus secara in vitro. Makin tinggi konsentrasi ekstrak, maka daya anthelmintiknya juga semakin tinggi. Pada ekstrak konsentrasi 10% memiliki kemampuan anthelmintik yang hampir sama dengan Albendazole.

The Prevalance and Helminth Infection Degree of Gastrointestinal in Layer Duck Located in Keper and Markolak Kramat Village District of Bangkalan Regency of Bangkalan Ana Amaliah; Indah Norma Triana; Poedji Hastutiek; Setiawan Koesdarto; Lucia Tri Suwanti; Soeharsono Soeharsono
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Tujuan dari penelitian ini adalah untuk menentukan prevalensi dan derajat infeksi cacing saluran pencernaan pada itik petelur di dua kondisi lingkungan yang berbeda. Sampel feses diperiksa dengan metode sedimentasi dan metode apung kemudian dilanjutkan dengan menghitung telur cacing per gram tinja dengan metode Mc Master. Hasil menunjukkan bahwa 22,9% (8/35) itik petelur di Dusun Keper terinfeksi oleh Capillaria sp., dan Echinostoma revolutum, dengan rata-rata derajat infeksi sebsar 52,50 ± 41,662, sedangkan 31,4% (11/35) itik petelur di Dusun Markolak terinfeksi oleh Capillaria sp., Echinostoma revolutum, dan cacing dari kelas cestoda, dengan rata-rata derajat infeksi sebesar 155,45 ± 166,395. Infeksi bersifat tunggal maupun campuran. Analisis terhadap prevalensi dan derajat infeksi cacing saluran pencernaan itik petelur menunujukkan hasil yang tidak signifikan (p>0,05) antara Dusun Keper dan Dusun Markolak.

The Effect of Calcium Hydroxideon Mortality of Ascaridia galli In Vitro Inggarsetya Syah Audini; Setiawan Koesdarto; Ira Sari Yudaniayanti; Setiawati Sigit; Sri Mumpuni Sosiawati; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Ascaridiasis can be happened in either broiler or hens that can caused decrease of meat and egg productions. Ascaridiasis is a parasite that is most often found in poultry and cause high economic losses. This research was conducted to determine the anthelmintic effect of calcium hydroxide [Ca(OH)₂] on mortality of Ascaridia galli in vitro. In this research used Ascaridia galli with length 7-11 cm without differentiating their sex. The concentrations of calcium hydroxide [Ca(OH)₂] for immersion the Ascaridia galli were 0,01%, 0,02%, 0,025% and 0,03% as treatment group, respectively. The negative control used aquadest. Observation death A.galli worm and analysis of the data at the 2 hour, 4 hour, 6 hour, 8 hour, 10 hour and 12 hour. The result showed that the increase of concentration increased mortality of Ascaridia galli. Percentage of death worm A.galli at the 12th hour on concentration ,01%, 0,02%, 0,025% and 0,03% was 20%, 55%, 75% and 100%. ANOVA test showed significant difference between control and treatment group (p<0.05). Duncan multiple range test was seen the best treatment was the biggest mortality in concentration of 0,03%.

Anthelmintic Activity Ethanol Extract of Ocimum sanctum Linn. Leaves Against Ascaridia galli In Vitro Vanna Lidya Kharisma; Setiawan Koesdarto; Koesnoto Supriandono; Lucia Tri Suwanti; Sri Agus Sudjarwo; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this research are to determine concentration, exposure time, interaction between concentration and exposure time of ethanol extract of Ocimum sanctum Linn. Leaves to cause death toward Ascaridia galli in vitro, and the value of LC50 and LC90 ethanol extract of Ocimum sanctum Linn. Leaves. Research design that has been used in the research was completely randomized design. This research used 200 samples of Ascaridia galli with length 7-11 cm without differentiating their sex. The concentration ethanol extract of Ocimum sanctum Linn. leaves were 1.25%, 2.5%, 5%, 10%. The control was using CMC-Na 0.5%. Each treatment then being replicated four times. The observation and recording of dead worm were done at 0, 3, 6, 12 and 24 hours. Ascaridia galli were declared dead if there was no movement while disturbed by anatomy tweezers and dipped in slightly warm water (50ºC). The obtained data was analyzed using Anova Factorial and continued with Duncan Multiple Range Test by SPSS for Windows 22. The result were 10% concentration and exposure time for 24 hours caused the most mortality toward Ascaridia galli. Interaction between concentration and exposure time resulted 10% concentration ethanol extract of Ocimum sanctum Linn. leaves in 24 hours caused the most mortality towards Ascaridia galli. Probit analysis was used to calculate the LC50 and LC90 of Ocimum sanctum Linn. leaves. The results were LC50 ethanol extract of Ocimum sanctum Linn. leaves at 6 hours was 14.8%, at 12 hours was 4.8% and at 24 hours was 3.0% and the LC90 at 24 hours was 9.1%.

Identification of Worm In Digestive Tract of Consumed Cattle Egret (Bubulcus ibis) In Ujung Pangkah, Gresik Nisa’ Rachmaningtyas Putri; E Djoko Poetranto; Nunuk Dyah Retno Lastuti; Setiawan Koesdarto; Bambang Sektiari Lukiswanto; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Ujung Pangkah, Gresik was became the most habitat of fowls such as Cattle egret (Bubulcus ibis). The aim of this research were to find out the type of worm that infected digestive tract of Cattle egret (Bubulcus ibis) in Ujung Pangkah, Gresik. The examination of worm in digestive tract was done by dissection and fecal examination. The identification based on morphological of the eggs and worms. The samples of digestive tract organ collected from restaurant that preserve Cattle egret as a main menu. Whereas the fecal sample collected by swab from the intestine. Cattle egret (Bubulcus ibis) has positive infection by worm parasite if there were worm or egg inside the digestive tract. Fecal samples were examined by native, sedimentation, coconut-sugar floating method and EPG counting with Lucient Brumpt. And the worm morphology from dissection of digestive tract would be identify with source book, journal, and discussed with supervisor. The result showed that 20 of digestive tract dissection and 20 fecal samples, there were three types of worm, Contracaecum sp., Cosmocephalus sp., Capillaria sp. and egg of Contracaecum sp. with 200 EPG

Efectiveness of Ocimum sanctum Linn. Leaves Ethanol Extract on Mortality Raillietina echinobothrida In Vitro Jessica Giovani; Setiawan Koesdarto; Mirny Lamid; Sri Agus Sudjarwo; Suryo Kuncorojakti
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of the research are to prove the anthelmintic effects of Ocimum sanctum Linn. leaves ethanol extract on Raillietina echinobothrida in vitro and to know the value of LC50 and LC90of Ocimum sanctum Linn. leaves ethanol extract. The research used completely randomized design. The research had five treatments and each treatment was done in four replications. The research used 5 Raillietina echinobothrida adult worms for each treatment and all replications. The observation and the recording were done at the first hour until the sixth hour of the research. The Raillietina echinobothrida adult worms were noted dead if there was no movement when they were disturbed by anatomic tweezer, the death of Raillietina echinobothrida were confirmed when they dipped mildly in warm water (50Â°C) and they showed no movements. The acquired data was analyzed using ANOVA and continued with LSD (5%). The results were Ocimum santum Linn. ethanol extract has the anthelmintic activity in 1.25%, 2.5%, 5% and 10% concentrations. The LC50 and LC90 was analyzed using probit analysis. The results of LC50 calculation were 7.8% at the third hour, 6% at the forth hour, 3.2% at the fifth hour, 2.5% at the sixth hour and the results of LC90 were 6.5% at the fifth hour, 5.2% at the sixth hour. The higher concentration of the extract, the higher anthelmintic activity worked.

Prevalence of Nematodiasis in Omasum and Abomasum of Goats Slaughtered at Pegirian Surabaya Slaughter House Through Gatrointestinal Surgery Method Dewi Mariyam; Sri Mumpuni Sosiawati; Kuncoro Puguh Santoso; Setiawan Koesdarto; Sarmanu Sarmanu; Ira Sari Yudaniayanti
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research is to determine the kind of nematodes and to determine the prevalence result. Thirtysix samples of omasum and abomasum were collected from RPH Pegirian from September-November 2017 then using gastrointestinal surgery to get fresh adult worm, afterwards using Semichen-Acetic Carmine stainning method to makes the object easy to microscopic identify. The result showed that species was found Haemonchus contortus, as an identify result show that adult worm has a character on a anterior part called cervical papilae, female worm has a barber’s pole on body also posterior part has a vulva flap, posterior part of male worm has a bursa copulatrix, spicula, gubernaculum and dorsal rays looks like Y shape. Seventeen positive samples and the prevalence rate was 47,2%, 273 adult worms and female worms 215 (78,75%) and the male worms 58 (21,25%). Chi-Square statistical test (P <0.05) showed that 13 positive samples of kambing kacang (65%) were higher infected Haemonchosis infection than 4 potitive samples of PE (25%).

Identification of Worm Eggs in Faeces of Egrets (Egretta sp.) in Surabaya Akbar Haryo Widhi Trismiharto; Suzanita Utama; Koesnoto Supranianondo; Djoko Poetranto; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research was to identify worm eggs species that infected Egrets in Surabaya. A total of 40 samples were taken and faecal examination was conducted in the Laboratory by means of native, sedimentation, and floating methods, In floating method saturated brown sugar solution was used as floating media because brown sugar solution was faster in floating worm eggs. Then further examination of the worm eggs species were matched by microscopic taxonomy and textbook comparison observation. Eggs of three classes of helmints, Trematode, Cestode, and Nematode were found in faeces of Egrets in Surabaya. Species of the Nematode eggs found were Ascaris sp., Ascaridia galli, , Capillaria sp., Toxocara cati. Species of Cestode class egg found were Raillietina sp, from Trematode class were Echinostoma revolutum.

Prevalence of Protozoa in Gastrointestinal Tract of Pigeons (Columba livia) Maintenance Ekstensif and Intensif in Surabaya Felita Widyaningsih; Rimayanti Rimayanti; Setiawan Koesdarto; Lucia Tri Suwanti; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

This research was conducted to determine the prevalence of major gastrointestinal protozoal infections, the kinds of protozoa that infects and maintenance systems influence on pigeons in Surabaya. Pigeon taken from five regions of Surabaya namely North Surabaya, East Surabaya, West Surabaya, South Surabaya and Surabaya Center. Each region is taken as many as 20 pigeons consisted of 10 pigeons with extensive maintenance systemÂ and 10 pigeons with intensif maintenance system. So that the total overall sample is 100 pigeons. Laboratory examination (microscopic) was conducted in November 2017 until January 2017. Examination of the upper gastrointestinal tract using throat swabs and stool samples were examined with native methods, sedimentation, and flotation. Identification using a microscope using 40x objective scale and 10x oculer scale to determine gastrointestinal protozoal that was found. Data was analyzed using Chi Square Test statistical analysis. Results showed 78% of the positive samples gastrointestinal protozoa infections in pigeons in the city of Surabaya. Results of Chi Square Test statistical analysis showed a p value of 0.030 (p<0.05) which means significantly different. Types of protozoa found that Trichomonas gallinae (49%) and Eimeria sp. (61%). The prevalence of gastrointestinal protozoal pigeons on extensive maintenance sistemÂ was higher (44%) compared to the intensive caresis system(34%).

Co-Authors Adiacahya, Eren Adiana Mutamsari Witaningrum Agus Wijaya Akbar Haryo Widhi Trismiharto Aksono HP., Eduardus Bimo Aldi Hamdani Amelia Dwita Safitri Ana Amaliah Anita Nur Aida Aida Annisa Novia Damayanti APRIL HARI WARDHANA Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azizatun Nimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Boedi Setiawan Bryan Ahmad Affan Lubis BUDI UTOMO Budiarto Budiarto Budiarto Budiarto Dadik Rahardjo, Dadik Dewi Mariyam Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Didik Handijatno Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Emy Koestanti Sabdoningrum Endang Suprihati Eny Coolfina Simarmata Felita Widyaningsih Firman Hadi Fanani Galuh Swa Ardhya Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Ida Yuniarti Indah Norma Triana, Indah Norma Indira Vania Carisya Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid jayawardhana, andi Jessica Giovani Karina Rachmawati Khairullah, Aswin Rafif Khairunnisa, Hanifa Khansa Khalissa Farah Alifia Kismiyati , Koesnoto Supranianondo Koesnoto Supriandono Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurnianto, Muhammad Aqil Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Mafruchati, Maslichah Mas’ud Hariadi Melani Anggraini Melanie Aulia Ashfiyah Meles, Dewa Ketut Mirni Lamid Mirny Lamid Mochamad Lazuardi Mohamad Safri Sauqi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Nenny Harijani Ngakan Made Rai Widjaja Nidom, Chairul Anwar Nila Murodah Nisa’ Rachmaningtyas Putri Nove Hidajati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurhusien Yimer Ogen Sea Poedji Hastutiek Poetranto, Emmanuel Djoko Praja, Ratih Novita Prasetya, Muhammad Rofi' Pratiwi, Arif Primarizky, Hardany Pudji Srianto Putri, Valerie Brilianda Rahadju Ernawati Rahaju Ernawati Rahardjo, Adi Prijo Rahmi Sugihartuti Rari Noviana Ratih Prajnya Paramitha Rekasni Adallin A/P Morgan A/P Morgan Rimayanti Rimayanti Risky Aprillian, Risky Rochmah Kurniasanti Santosa, Gusti Ayu Illiyin Putri Sari, Aulia Puspita Sari, Putri Desi Wulan Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawati Sigit Soeharsono Soeharsono Soelih Estoepangestie, Agnes Theresia Sofie Adi, Keren Jayanti Sri Agus Sudjarwo Sri Mumpuni Sosiawati Sri Mumpuni Sosiawati Sunarso, Agus Suwarno Suwarno Suzanita Utama Syah Putra, Akyun Rozaqi Tanjung, Aldi Gusnizar Rizaldy Tita Damayanti Lestari Tjuk Imam Restiadi Trifena Pristi Anindyta Tyasningsih, Wiwiek Tyasningsih, Wiwik Vanna Lidya Kharisma Vindo Rossy Pertiwi Virgy Dzakina Fahrezy Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla

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