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All Journal Jurnal Sain Veteriner Jurnal Veteriner Indonesian Journal of Tropical and Infectious Disease Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Journal of Fisheries & Marine Jurnal Agro Veteriner
Setiawan Koesdarto
Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Ratih Novita Praja; Didik Handijatno; Setiawan Koesdarto; Aditya Yudhana
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.411 KB) | DOI: 10.21157/j.ked.hewan.v12i1.8091

Abstract

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.
Identification and Prevalence of Endoparasite on Layer Chicken in Udanawu Sub-district Blitar Khalissa Farah Alifia; Setiawan Koesdarto; Yulianna Puspitasari; Mufasirin; Adiana Mutamsari Witaningrum
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.38769

Abstract

The aim of this research was to determined the prevalence and infection degrees of endoparasite on layer chicken in Sub-district Udanawu, Blitar. Ninety-six samples were taken from layer chicken in 3 different villages namely Bakung Village, Tunjung Village, and Slemanan Village. The examinations taken in this study are fecal examination using native, sediment, and floating methods and blood examination using blood smear method. Result showed that 81.25% samples are positive for helminthiasis infection consisting of Ascaridia galli (66.67%), Heterakis gallinarum (45.83%), Raillietina sp. (31.25%), and Strongyloides avium (7.29%). Blood examination result shown there is no positive sample that infect layer chicken in Sub-district Udanawu, Blitar. Chi-Square test result showed there are significant difference (P<0.05) of Ascaridia galli and Heterakis gallinarum in Bakung Village, Tunjung Village, and Slemanan Village in Sub-district Udanawu, meanwhile there are no significant difference (P>0.05) of Raillietina sp. and Strongyloides avium. Range of infection degrees of helminthiasis in Bakung village, Tunjung village, and Slemanan Village are 608.75 ± 588.53, 223.12 ± 359.21, 156.25 ± 332.39. There are significant difference (P<0.05) on helminthiasis infection degree of layer chicken in Udanawu District, Blitar.
Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Muhammad Rofi&#039; Prasetya; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Kusnoto Kusnoto; Muchammad Yunus
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18201

Abstract

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.
Perubahan Patologi Kulit Ikan Gurami (Osphronemu gouramy) Akibat Infestasi Lernaea cyprinacea Pada Derajat Infestasi Yang Berbeda [The Pathology Alteration Of Gouramy (Osphronemus gouramy) Integument Infested By Lernaea cyprinacea At Different Degress Of Infestation] Gunanti Mahasri; Putri Desi Wulan Sari; Setiawan Koesdarto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11640

Abstract

Abstract The development of fish consumption in Indonesia was promising increasingly and growing very rapidly. One of the priority commodities in the development of aquaculture subsector was the gourami (Osphronemus gouramy). Many factors must be faced in the cultivation of gouramy, including the problem of fish diseases caused by Lernaea cyprinacea. The aim of this research is to find out the description of anatomic pathology and histopathology of gouramy which infested by L. cyprinacea at different degrees of infestation. This research uses descriptive method. The research showed that the gouramy integument which infested by L. cyprinacea at low, moderate and heavy infestation cause moderate until heavy defect with 2.48; 2.42 and 2.32 in reaverage scoring. Hence, accordance with pathological anatomy, L. cyprinacea infestation caused necrotic nodules, lesion which covered by exudate and haemorrhage. While the histopathology of the integument changes found were inflammatory cell infiltration, congestion and haemorrhage in the low, moderate and heavy infestation.
EVALUATION OF Salmonella sp CONTAMINATION AND ITS ANTIBIOTICS RESISTANCE PATTERNS ISOLATED FROM BROILER MEAT SOLD AT WET MARKET IN CENTER OF SURABAYA Aprillian, Risky; Rahardjo, Dadik; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 6 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.339 KB) | DOI: 10.20473/ijtid.v5i6.440

Abstract

Antibiotic resistance now days become a main issue to the medical researches as found many positive result of antibiotic resistance test. One of the causes of antibiotic resistance is using antibiotic as a feed additive to animal. Bacteria that are resistant to antibiotics can be a danger to humans, in this case the resistant bacteria as a result of treatment errors animals, especially chickens that uses low-dose antibiotics as growth promoters. This study aimed to determine the contamination of Salmonella sp and its antibiotics resistance patterns of Salmonella sp isolated from broiler meat sold at wet market in the Center of Surabaya: (a) Pasar Kembang, (b) Pasar Kupang, (c) Pasar Dukuh Kupang, (d) Pasar Kedungsari, (e) Pasar Kedungdoro and (f) Pasar Keputran. The method that used in this study was bacteriological isolation and identification method. The method started with pre-enrichment using Buffered Pepton Water, selective enrichment using Tetrathionate Broth and Selenite Cysteine broth, selective media using Salmonella-Shigella Agar, Biochemical test using Triple Sugar Iron Agar, Simon Citrate, Methyl Red – Voges Proskauer, and Sulfide Indol Motility, and followed with susceptibility test according to Kirby-Bauer method using Mueller-Hinton Agar. The antibiotics that used in susceptibility test were: (a) Meropenem, (b) Ampicillin Sulbactam, (c) Amikacin, (d) Ofloxacin and (e) Nalidixic Acid. The results of this study were found 90% or 27 of 30 samples positive contaminated with Salmonella sp. The results of antibiotics resistance from 27 isolates 0% were resistant to Meropenem, 0% were resistant to Amikacin; 3.7% were resistant to Ampicillin-Sulbactam; 11.1% were resistant to Ofloxacin and 44.4% were resistant to Nalidixic Acid.
THE IMMUNOSTIMULAN POTENTIAL OF TENGGULUN (PROTIUM JAVANICUM) LEAVES TOWARDS T CELL CD4+ AND IFNγ SECRETION ON PBMC CHICKEN Jayawardhana, Andi; Puspitasari, Heni; Meles, Dewa Ketut; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 3 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.478 KB) | DOI: 10.20473/ijtid.v6i3.2992

Abstract

One of the plants with immunostimulant activity is Tenggulun leaves which contain of flavonoid, like terpenoid. The aim of this research is to find the potential of Tenggulun's leaves extract to have the immunostimulant activities. The potential of immunostimulant activity is identified by the increasing the amount of T-cell CD4+ expression and IFNγ secretion. The research method is conducted through cultured chicken PBMC which is infected by ND virus; it is then treated with Tenggulun's leaves extract with immunostimulant. The result of immunocitochemistry examination CD4+ secretion on PBMC cultures shows how tenggulun is significantly different from the control in the secretion CD4+. The 10μg of tenggulun extract can modulate the T cell CD4+ secretion 68.8±0.83. It is significantly different from K (control) (p<0,05) and treatment group K+, P0, and P1. The examination of IFNγ level using ELISA from tenggulun leaves extract of 10μg doses were inoculated after being infected by ND virus contained immonostimulant potential in increasing the secretion of IFNγ 120.91±6.44. It is significantly different from K-, K+, and P1, yet not significantly different from P0. The content of terpenoid can increase IFNγ secretion on the macrofag cells culture and limfosit cells. 
Identifikasi Molekuler Blastocystis sp. pada Monyet Ekor Panjang (Macaca fascicularis) di Taman Nasional Baluran, Situbondo, Jawa Timur Kurniawati, Dyah Ayu; Suwanti, Lucia Tri; Lastuti, Nunuk Dyah Retno; Koesdarto, Setiawan; Suprihati, Endang; Mufasirin, Mufasirin; Pratiwi, Arif
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.138-144

Abstract

Interaksi yang dekat antara monyet ekor panjang dengan manusia dapat meningkatkan risiko penularan penyakit zoonosis. Blastocystis sp. adalah protozoa gastrointestinal pada manusia dan hewan yang yang bersifat zoonosis. Penelitian ini bertujuan untuk mengidentifikasi Blastocystis sp. yang menginfeksi monyet ekor panjang melalui identifikasi molekuler. Identifikasi Blastocystis sp. pada penelitian ini menggunakan metode morfologis dan molekuler. Sebanyak 90 feses individu monyet ekor panjang Taman Nasional Baluran dilakukan pemeriksaan secara mikroskopis setelah dilakukan kultur pada Jones Medium. 28 dari sampel yang positif secara mikroskopis dilanjutkan dengan uji PCR dengan target primer barcode region yang mempunyai visualisasi 600bp. Tiga sampel dengan band positif 600bp dilanjutkan dengan squencing. Hasil sekuens diproses dalam BLAST dan MLST. Satu sampel yang terkonfirmasi sebagai Blastocystis sp. dengan infeksi campuran dari subtipe 1 alel 2 dan subtipe 3 alel 34. Hasil menunjukkan bahwa Blastocystis sp. terdapat pada monyet ekor panjang di Taman Nasional Baluran dengan prevalensi rendah.
Detection of Spargana on Ptyas korros in Sidoarjo Bagaskara, Ryan; Lukiswanto, Bambang Sektiari; Rahardjo, Adi Prijo; Poetranto, Emmanuel Djoko; Koesdarto, Setiawan; Mufasirin, Mufasirin
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.122-125

Abstract

Ptyas korros or Indo-China Rat Snake is one of the snakes that are commonly hunted in Indonesia. This study aimed to identify Spirometra sp. on P. korros in Sidoarjo. A total of 18 snakes were collected within 3 months. Identification of Spirometra sp. was done using a native method, Semichen Acetic Carmine, and HE staining. As a results, 8 (44.4%) of 18 samples showed positive infection with Spirometra sp. The results of this study indicate the presence of spargana in P. korros in Sidoarjo and no adult Spirometra sp. was found.
Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in Sidoarjo, Indonesia Sari, Aulia Puspita; Koesdarto, Setiawan; Lestari, Tita Damayanti; Hastutiek, Poedji; Yunus, Muchammad; Herupradoto, Eduardus Bimo Aksono; Khairullah, Aswin Rafif; Ayuti, Siti Rani; Hasib, Abdullah; Aryaloka, Suhita; Moses, Ikechukwu Benjamin
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.370-381

Abstract

The problem most often encountered in fish farming is disease which can cause a decrease in fish production levels. Parasites cause one of the infectious diseases found in fish farming. The presence of parasites can cause a decrease in the quality of the growth of fish production. The purpose of this study was to identify the prevalence of ectoparasites on Nile tilapia in Sidoarjo, Indonesia. Ninety-six samples from two ponds were examined under the microscope to find out the presence of ectoparasite that exists in the outside body part of the fish. Samples were collected from skin, fins, tail, and gills using native methods and observed under a microscope in the Division of Parasitology Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. The results of this study showed 86 samples positively infected by ectoparasite and 10 showed negative. The ectoparasites found in this study were Trichodina sp., Chilodonella sp., Ichthyophthirius multifillis, Gyrodactylus sp., and Dactylogyrus sp. The highest prevalence is Trichodina sp. and Dactylogyrus sp. The number of fish infested by ectoparasite is 89.56%, this is classified in the usual category. The highest prevalence occurs in infestations of Trichodina sp. i.e.72.91% which were contained in the 70 samples. While the lowest prevalence was in Gyrodactylus sp. by 8.32% in 8 fish samples. Double infections from two different ponds were 70% within 33 samples which were included in the usual category.
IDENTIFIKASI BAKTERI Escherichia coli PENGHASIL Extended Spectrum β-Lactamase DARI SWAB RECTAL SAPI PERAH MENGGUNAKAN METODE VITEK-2 DI KUD TANI WILIS SENDANG KABUPATEN TULUNGAGUNG Syah Putra, Akyun Rozaqi; Effendi, Mustofa Helmi; Koesdarto, Setiawan; Suwarno, Suwarno; Tyasningsih, Wiwik; Soelih Estoepangestie, Agnes Theresia
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20414

Abstract

Antibiotic resistance in animals and humans has become a global problem that needs attention. The use of antibiotics in inappropriate on food-producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). The aim of this study was to isolate and identified ESBL- E. coli isolate from dairy cow rectal swabs in Sendang, Tulungagung district using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The result of the study showed that from all of the samples could be isolated and indentified E. coli, based on the colony characteristics on EMBA and biochemical test. Based on the double disc synergy test method using antibiotic disc amoxicylyn-clavulanate, ceftriaxone, aztreonam, ceftazidime and cefotaxime, 10 isolates could be identify els ESBL- E. coli. furthermore 3 out of 10 isolates DDST positives were confirmed ESBL- E. coli using Vitek-2 method.
Co-Authors Adiacahya, Eren Adiana Mutamsari Witaningrum Agus Wijaya Akbar Haryo Widhi Trismiharto Aksono HP., Eduardus Bimo Aldi Hamdani Amelia Dwita Safitri Ana Amaliah Anita Nur Aida Aida Annisa Novia Damayanti APRIL HARI WARDHANA Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azizatun Nimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Boedi Setiawan Bryan Ahmad Affan Lubis BUDI UTOMO Budiarto Budiarto Budiarto Budiarto Dadik Rahardjo, Dadik Dewi Mariyam Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Didik Handijatno Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Emy Koestanti Sabdoningrum Endang Suprihati Eny Coolfina Simarmata Felita Widyaningsih Firman Hadi Fanani Galuh Swa Ardhya Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Ida Yuniarti Indah Norma Triana, Indah Norma Indira Vania Carisya Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid jayawardhana, andi Jessica Giovani Karina Rachmawati Khairullah, Aswin Rafif Khairunnisa, Hanifa Khansa Khalissa Farah Alifia Kismiyati , Koesnoto Supranianondo Koesnoto Supriandono Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurnianto, Muhammad Aqil Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Mafruchati, Maslichah Mas’ud Hariadi Melani Anggraini Melanie Aulia Ashfiyah Meles, Dewa Ketut Mirni Lamid Mirny Lamid Mochamad Lazuardi Mohamad Safri Sauqi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Rofi&#039; Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Nenny Harijani Ngakan Made Rai Widjaja Nidom, Chairul Anwar Nila Murodah Nisa’ Rachmaningtyas Putri Nove Hidajati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurhusien Yimer Ogen Sea Poedji Hastutiek Poetranto, Emmanuel Djoko Praja, Ratih Novita Prasetya, Muhammad Rofi' Pratiwi, Arif Primarizky, Hardany Pudji Srianto Putri, Valerie Brilianda Rahadju Ernawati Rahaju Ernawati Rahardjo, Adi Prijo Rahmi Sugihartuti Rari Noviana Ratih Prajnya Paramitha Rekasni Adallin A/P Morgan A/P Morgan Rimayanti Rimayanti Risky Aprillian, Risky Rochmah Kurniasanti Santosa, Gusti Ayu Illiyin Putri Sari, Aulia Puspita Sari, Putri Desi Wulan Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawati Sigit Soeharsono Soeharsono Soelih Estoepangestie, Agnes Theresia Sofie Adi, Keren Jayanti Sri Agus Sudjarwo Sri Mumpuni Sosiawati Sri Mumpuni Sosiawati Sunarso, Agus Suwarno Suwarno Suzanita Utama Syah Putra, Akyun Rozaqi Tanjung, Aldi Gusnizar Rizaldy Tita Damayanti Lestari Tjuk Imam Restiadi Trifena Pristi Anindyta Tyasningsih, Wiwiek Tyasningsih, Wiwik Vanna Lidya Kharisma Vindo Rossy Pertiwi Virgy Dzakina Fahrezy Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla