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Perubahan Patologi Kulit Ikan Gurami (Osphronemu gouramy) Akibat Infestasi Lernaea cyprinacea Pada Derajat Infestasi Yang Berbeda [The Pathology Alteration Of Gouramy (Osphronemus gouramy) Integument Infested By Lernaea cyprinacea At Different Degress Of Infestation] Gunanti Mahasri; Putri Desi Wulan Sari; Setiawan Koesdarto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11640

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Abstract The development of fish consumption in Indonesia was promising increasingly and growing very rapidly. One of the priority commodities in the development of aquaculture subsector was the gourami (Osphronemus gouramy). Many factors must be faced in the cultivation of gouramy, including the problem of fish diseases caused by Lernaea cyprinacea. The aim of this research is to find out the description of anatomic pathology and histopathology of gouramy which infested by L. cyprinacea at different degrees of infestation. This research uses descriptive method. The research showed that the gouramy integument which infested by L. cyprinacea at low, moderate and heavy infestation cause moderate until heavy defect with 2.48; 2.42 and 2.32 in reaverage scoring. Hence, accordance with pathological anatomy, L. cyprinacea infestation caused necrotic nodules, lesion which covered by exudate and haemorrhage. While the histopathology of the integument changes found were inflammatory cell infiltration, congestion and haemorrhage in the low, moderate and heavy infestation.
EVALUATION OF Salmonella sp CONTAMINATION AND ITS ANTIBIOTICS RESISTANCE PATTERNS ISOLATED FROM BROILER MEAT SOLD AT WET MARKET IN CENTER OF SURABAYA Aprillian, Risky; Rahardjo, Dadik; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol. 5 No. 6 (2015)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (216.339 KB) | DOI: 10.20473/ijtid.v5i6.440

Abstract

Antibiotic resistance now days become a main issue to the medical researches as found many positive result of antibiotic resistance test. One of the causes of antibiotic resistance is using antibiotic as a feed additive to animal. Bacteria that are resistant to antibiotics can be a danger to humans, in this case the resistant bacteria as a result of treatment errors animals, especially chickens that uses low-dose antibiotics as growth promoters. This study aimed to determine the contamination of Salmonella sp and its antibiotics resistance patterns of Salmonella sp isolated from broiler meat sold at wet market in the Center of Surabaya: (a) Pasar Kembang, (b) Pasar Kupang, (c) Pasar Dukuh Kupang, (d) Pasar Kedungsari, (e) Pasar Kedungdoro and (f) Pasar Keputran. The method that used in this study was bacteriological isolation and identification method. The method started with pre-enrichment using Buffered Pepton Water, selective enrichment using Tetrathionate Broth and Selenite Cysteine broth, selective media using Salmonella-Shigella Agar, Biochemical test using Triple Sugar Iron Agar, Simon Citrate, Methyl Red – Voges Proskauer, and Sulfide Indol Motility, and followed with susceptibility test according to Kirby-Bauer method using Mueller-Hinton Agar. The antibiotics that used in susceptibility test were: (a) Meropenem, (b) Ampicillin Sulbactam, (c) Amikacin, (d) Ofloxacin and (e) Nalidixic Acid. The results of this study were found 90% or 27 of 30 samples positive contaminated with Salmonella sp. The results of antibiotics resistance from 27 isolates 0% were resistant to Meropenem, 0% were resistant to Amikacin; 3.7% were resistant to Ampicillin-Sulbactam; 11.1% were resistant to Ofloxacin and 44.4% were resistant to Nalidixic Acid.
THE IMMUNOSTIMULAN POTENTIAL OF TENGGULUN (PROTIUM JAVANICUM) LEAVES TOWARDS T CELL CD4+ AND IFNγ SECRETION ON PBMC CHICKEN Jayawardhana, Andi; Puspitasari, Heni; Meles, Dewa Ketut; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 3 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.478 KB) | DOI: 10.20473/ijtid.v6i3.2992

Abstract

One of the plants with immunostimulant activity is Tenggulun leaves which contain of flavonoid, like terpenoid. The aim of this research is to find the potential of Tenggulun's leaves extract to have the immunostimulant activities. The potential of immunostimulant activity is identified by the increasing the amount of T-cell CD4+ expression and IFNγ secretion. The research method is conducted through cultured chicken PBMC which is infected by ND virus; it is then treated with Tenggulun's leaves extract with immunostimulant. The result of immunocitochemistry examination CD4+ secretion on PBMC cultures shows how tenggulun is significantly different from the control in the secretion CD4+. The 10μg of tenggulun extract can modulate the T cell CD4+ secretion 68.8±0.83. It is significantly different from K (control) (p<0,05) and treatment group K+, P0, and P1. The examination of IFNγ level using ELISA from tenggulun leaves extract of 10μg doses were inoculated after being infected by ND virus contained immonostimulant potential in increasing the secretion of IFNγ 120.91±6.44. It is significantly different from K-, K+, and P1, yet not significantly different from P0. The content of terpenoid can increase IFNγ secretion on the macrofag cells culture and limfosit cells. 
Identifikasi Molekuler Blastocystis sp. pada Monyet Ekor Panjang (Macaca fascicularis) di Taman Nasional Baluran, Situbondo, Jawa Timur Kurniawati, Dyah Ayu; Suwanti, Lucia Tri; Lastuti, Nunuk Dyah Retno; Koesdarto, Setiawan; Suprihati, Endang; Mufasirin, Mufasirin; Pratiwi, Arif
Jurnal Medik Veteriner Vol. 3 No. 2 (2020): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol3.iss2.2020.138-144

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Interaksi yang dekat antara monyet ekor panjang dengan manusia dapat meningkatkan risiko penularan penyakit zoonosis. Blastocystis sp. adalah protozoa gastrointestinal pada manusia dan hewan yang yang bersifat zoonosis. Penelitian ini bertujuan untuk mengidentifikasi Blastocystis sp. yang menginfeksi monyet ekor panjang melalui identifikasi molekuler. Identifikasi Blastocystis sp. pada penelitian ini menggunakan metode morfologis dan molekuler. Sebanyak 90 feses individu monyet ekor panjang Taman Nasional Baluran dilakukan pemeriksaan secara mikroskopis setelah dilakukan kultur pada Jones Medium. 28 dari sampel yang positif secara mikroskopis dilanjutkan dengan uji PCR dengan target primer barcode region yang mempunyai visualisasi 600bp. Tiga sampel dengan band positif 600bp dilanjutkan dengan squencing. Hasil sekuens diproses dalam BLAST dan MLST. Satu sampel yang terkonfirmasi sebagai Blastocystis sp. dengan infeksi campuran dari subtipe 1 alel 2 dan subtipe 3 alel 34. Hasil menunjukkan bahwa Blastocystis sp. terdapat pada monyet ekor panjang di Taman Nasional Baluran dengan prevalensi rendah.
Detection of Spargana on Ptyas korros in Sidoarjo Bagaskara, Ryan; Lukiswanto, Bambang Sektiari; Rahardjo, Adi Prijo; Poetranto, Emmanuel Djoko; Koesdarto, Setiawan; Mufasirin, Mufasirin
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.122-125

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Ptyas korros or Indo-China Rat Snake is one of the snakes that are commonly hunted in Indonesia. This study aimed to identify Spirometra sp. on P. korros in Sidoarjo. A total of 18 snakes were collected within 3 months. Identification of Spirometra sp. was done using a native method, Semichen Acetic Carmine, and HE staining. As a results, 8 (44.4%) of 18 samples showed positive infection with Spirometra sp. The results of this study indicate the presence of spargana in P. korros in Sidoarjo and no adult Spirometra sp. was found.
Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in Sidoarjo, Indonesia Sari, Aulia Puspita; Koesdarto, Setiawan; Lestari, Tita Damayanti; Hastutiek, Poedji; Yunus, Muchammad; Herupradoto, Eduardus Bimo Aksono; Khairullah, Aswin Rafif; Ayuti, Siti Rani; Hasib, Abdullah; Aryaloka, Suhita; Moses, Ikechukwu Benjamin
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.370-381

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The problem most often encountered in fish farming is disease which can cause a decrease in fish production levels. Parasites cause one of the infectious diseases found in fish farming. The presence of parasites can cause a decrease in the quality of the growth of fish production. The purpose of this study was to identify the prevalence of ectoparasites on Nile tilapia in Sidoarjo, Indonesia. Ninety-six samples from two ponds were examined under the microscope to find out the presence of ectoparasite that exists in the outside body part of the fish. Samples were collected from skin, fins, tail, and gills using native methods and observed under a microscope in the Division of Parasitology Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. The results of this study showed 86 samples positively infected by ectoparasite and 10 showed negative. The ectoparasites found in this study were Trichodina sp., Chilodonella sp., Ichthyophthirius multifillis, Gyrodactylus sp., and Dactylogyrus sp. The highest prevalence is Trichodina sp. and Dactylogyrus sp. The number of fish infested by ectoparasite is 89.56%, this is classified in the usual category. The highest prevalence occurs in infestations of Trichodina sp. i.e.72.91% which were contained in the 70 samples. While the lowest prevalence was in Gyrodactylus sp. by 8.32% in 8 fish samples. Double infections from two different ponds were 70% within 33 samples which were included in the usual category.
IDENTIFIKASI BAKTERI Escherichia coli PENGHASIL Extended Spectrum β-Lactamase DARI SWAB RECTAL SAPI PERAH MENGGUNAKAN METODE VITEK-2 DI KUD TANI WILIS SENDANG KABUPATEN TULUNGAGUNG Syah Putra, Akyun Rozaqi; Effendi, Mustofa Helmi; Koesdarto, Setiawan; Suwarno, Suwarno; Tyasningsih, Wiwik; Soelih Estoepangestie, Agnes Theresia
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20414

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Antibiotic resistance in animals and humans has become a global problem that needs attention. The use of antibiotics in inappropriate on food-producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). The aim of this study was to isolate and identified ESBL- E. coli isolate from dairy cow rectal swabs in Sendang, Tulungagung district using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The result of the study showed that from all of the samples could be isolated and indentified E. coli, based on the colony characteristics on EMBA and biochemical test. Based on the double disc synergy test method using antibiotic disc amoxicylyn-clavulanate, ceftriaxone, aztreonam, ceftazidime and cefotaxime, 10 isolates could be identify els ESBL- E. coli. furthermore 3 out of 10 isolates DDST positives were confirmed ESBL- E. coli using Vitek-2 method.
White Kupang Flour (Corbula faba hinds), Fish Meal and Coconut Oil Usage on the Broilers Carcass Percentage and Abdominal Fat Virgy Dzakina Fahrezy; Mirni Lamid; Setiawan Koesdarto; Emy Koestanti Sabdoningrum; Widya Paramita Lokapirnasari; Sunaryo Hadi Warsito
Jurnal Agro Veteriner Vol. 7 No. 1 (2023): Jurnal Agro Veteriner
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/agrovet.v7i1.51393

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This study was conducted to determine the effect of using white mussel flour fish flour and coconut oil on carcass percentage and abdominal fat percentage in broilers. This study used a completely randomized design consist 4 treatments and 5 replications. The study used DOC ( Day Old Chick ) with the strain Lohman MB 202 Platinum for 20 animals . The treatment in this study was ration without using white mussel flour (P0), ration using 10% white mussel flour (P1), ration using 12.5% white mussel flour (P2), ration using 15% white mussel flour (P3). Variables observed were carcass percentage and abdominal fat percentage. Data were analyzed by Analysis of Variance (ANOVA) if there is a significant effect then proceed with Duncan's Multiple range test significance level of 5%. The results of the analysis showed that P3 showed a significant difference with the control treatment (P0) and treatment P1 (p<0.05). The best broiler carcass percentage was in treatment P3 (76.02%) and P2 (74.7%) while the lowest was in treatment P0 (71.20%). The results of Duncan's test on the percentage of abdominal fat showed that the control treatment (P0), treatment P1, treatment P2, and treatment P3 were not significantly different (p> 0.05).
First Report of Blood Protozoa Infection in Wild-Caught Asian Vine Snake (Ahaetulla prasina) Putri, Valerie Brilianda; Koesdarto, Setiawan; Yudhana, Aditya; Wardhana, April Hari; Wibawati, Prima Ayu; Santosa, Gusti Ayu Illiyin Putri; Tanjung, Aldi Gusnizar Rizaldy; Kurnianto, Muhammad Aqil; Khairunnisa, Hanifa Khansa
Journal of Basic Medical Veterinary Vol. 14 No. 1 (2025): Journal of Basic Medical Veterinary, June 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i1.69671

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The Asian vine snake (Ahaetulla prasina) is an arboreal snake that can inhabit various types of trees in both lowland and highland areas. Based on its habitat, the Asian vine snake has a high potential to be infected by parasites. One of the parasitic diseases that can infect snakes is blood protozoa, which have the potential to be zoonotic. It can be transmitted between animals and humans through food contamination due to environmental pollution by snake feces which contain infective stage of protozoa. Clinical symptoms of this infection include lethargy, dehydration, and anorexia, which are common signs of parasitism. Case reports related to blood protozoa in snakes in the Indonesia are still very limited and have never been reported in Asian vine snake, even though many people keep Asian vine snake as exotic pets.
Reproduction record of captive Sumatera elephant (Elephas maximus sumatranus) at Way Kambas National Park, Indonesia Sofie Adi, Keren Jayanti; Budiarto Budiarto; Hermin Ratnani; Pudji Srianto; Budi Utomo; Suzanita Utama; Setiawan Koesdarto; Nurhusien Yimer
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.157-165

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This research aims to determine reproductive data on Sumatran elephants (Elephas maximus sumatranus) in the ETC and ERU of Way Kambas National Park, Indonesia during 1988-2021. Data recorded from the elephant population at both locations (ETC and ERU) includes the number, gender of elephants, elephant calves, ages and birth dates. Calving intervals and service periods were calculated from calving records of cows with a minimum parity of two. Data collection produced data on 47 elephant calves from 13 female elephants with at least twice the parity and 12 primiparous cows. Elephant reproductive records at ETC and ERU were: age (37.44 ± 9.03 vs. 29.75 ± 3.30 years), parity (2.78 ± 1.09 vs. 2.50 ± 0.58), and age at first birth (18, 11 ± 3.92 vs. 17.75 ± 0.50 years). While the calving interval was 1857.56 ± 870.81 vs. 1833.00 ± 305.18 days, and service period respectively 1229.44 ± 846.18 vs. 1210.50 ± 283.59 days, respectively. It can be concluded that the calving interval and service period for captive elephants at ETC and ERU were not much different and were within the normal range. Young cows showed better reproductive efficiency than older cows. Monitoring calves is very important. Further assistance is needed to improve elephant mobile veterinary services, increase diagnostic laboratory capacity, and educate camp managers, veterinary assistants, and mahouts about elephant diseases, their monitoring and treatment. In addition, the use of reproductive technology such as monitoring ovulation using ultrasound and carrying out artificial insemination was expected to increase reproductive efficiency.
Co-Authors Adiacahya, Eren Adiana Mutamsari Witaningrum Agnes Theresia Soelih Estoepangestie Agus Wijaya Akbar Haryo Widhi Trismiharto Aksono HP., Eduardus Bimo Aldi Hamdani Amelia Dwita Safitri Ana Amaliah Anita Nur Aida Aida Annisa Novia Damayanti APRIL HARI WARDHANA Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azizatun Nimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Boedi Setiawan Bryan Ahmad Affan Lubis BUDI UTOMO Budiarto Budiarto Budiarto Budiarto Dadik Rahardjo, Dadik Dewi Mariyam Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Emy Koestanti Sabdoningrum Endang Suprihati Eny Coolfina Simarmata Felita Widyaningsih Firman Hadi Fanani Galuh Swa Ardhya Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Ida Yuniarti Indah Norma Triana, Indah Norma Indira Vania Carisya Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid jayawardhana, andi Jessica Giovani Karina Rachmawati Khairullah, Aswin Rafif Khairunnisa, Hanifa Khansa Khalissa Farah Alifia Kismiyati , Koesnoto Supranianondo Koesnoto Supriandono Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurnianto, Muhammad Aqil Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Mafruchati, Maslichah Mas’ud Hariadi Melani Anggraini Melanie Aulia Ashfiyah Meles, Dewa Ketut Mirni Lamid Mirny Lamid Mochamad Lazuardi Mohamad Safri Sauqi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Rofi&#039; Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Nenny Harijani Ngakan Made Rai Widjaja Nidom, Chairul Anwar Nila Murodah Nisa’ Rachmaningtyas Putri Nove Hidajati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurhusien Yimer Ogen Sea Poedji Hastutiek Poetranto, Emmanuel Djoko Praja, Ratih Novita Prasetya, Muhammad Rofi' Pratiwi, Arif Primarizky, Hardany Pudji Srianto Putri, Valerie Brilianda Rahadju Ernawati Rahaju Ernawati Rahardjo, Adi Prijo Rahmi Sugihartuti Rari Noviana Ratih Prajnya Paramitha Rekasni Adallin A/P Morgan A/P Morgan Rimayanti Rimayanti Risky Aprillian, Risky Rochmah Kurniasanti Santosa, Gusti Ayu Illiyin Putri Sari, Aulia Puspita Sari, Putri Desi Wulan Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawati Sigit Soeharsono Soeharsono Sofie Adi, Keren Jayanti Sri Agus Sudjarwo Sri Mumpuni Sosiawati Sri Mumpuni Sosiawati Sunarso, Agus Suwarno Suwarno Suzanita Utama Syah Putra, Akyun Rozaqi Tanjung, Aldi Gusnizar Rizaldy Tita Damayanti Lestari Tjuk Imam Restiadi Trifena Pristi Anindyta Tyasningsih, Wiwiek Tyasningsih, Wiwik Vanna Lidya Kharisma Vindo Rossy Pertiwi Virgy Dzakina Fahrezy Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla