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All Journal Jurnal Sain Veteriner Indonesian Journal of Tropical and Infectious Disease Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Journal of Fisheries & Marine Jurnal Agro Veteriner

Setiawan Koesdarto

Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 515120

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Anthelmintic Activity Test of Pare Leaves Extract (Momordica charantia L.) Against Worms Raillietina sp. by In Vitro Trifena Pristi Anindyta; Setiawan Koesdarto; Sri Agus Sudjarwo; Lilik Maslachah; mufasirin mufasirin; Nunuk Dyah Retno Lastuti; Lutfiah Annisa Billa
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research is to prove that pare leaf extract (Momordica charantia L.) has anthelmintic activity against Raillietina sp. in vitro. This study consisted of four treatments and each treatment contained six replications, each of which consisted of six worms. The treatments in this research were K- with 1% CMC-Na, P1 with 2% extract concentration, P2 with 4% extract concentration, P3 with 8% extract concentration. Pare leaf extract has anthelmintic activity against Raillietina sp. in vitro with an effective dose of 8%. The higher the concentration of pare leaf extract, the higher the mortality rate of worms. The longer the immersion time, the higher the number of dead worms.

Identifikasi dan Predileksi Ektoparasit Lernaea yang Menyerang Ikan Gurami (Osphronemus gouramy, Lac) Di Desa Ngrajek Kecamatan Mungkid Kabupaten Magelang Annisa Novia Damayanti; Kismiyati Kismiyati; Setiawan Koesdarto
Journal of Aquaculture and Fish Health Vol. 2 No. 1 (2013): JAFH Vol 2 No 1 Februari 2013
Publisher : Department of Aquaculture

Ikan gurami menjadi salah satu komoditas yang banyak dikembangkan oleh para petani karena permintaan pasar cukup tinggi, pemeliharaan mudah dan harga yang relatif stabil. Ikan gurami mempunyai bentuk badan agak panjang, pipih dan tertutup sisik yang berukuran besar serta terlihat kasar dan kuat. Keberhasilan suatu usaha budidaya ikan tidak terlepas dari masalah penyakit pada ikan. Keberadaan ektoparasit dapat menyebabkan penurunan kualitas tampilan ikan sehingga dapat menurunkan nilai jual ikan. Hal ini disebabkan karena parasit menyebabkan perubahan warna ikan dan kerusakan bagian tubuh ikan. Salah satu parasit yang sering menjangkit ikan adalah Lernaea yang menyerang ikan gurami. Tujuan penelitian ini adalah untuk mengidentifikasi ektoparasit Lernaea dan predileksinya pada ikan gurami (O. gouramy, Lac) di Desa Ngrajek, Magelang. Metode yang digunakan adalah metode survei dan pengambilan sampel menggunakan metode purpose random sampling. Data yang diperoleh disajikan secara deskriptif yaitu penyajian data dengan memaparkan data dalam bentuk penjelasan, angka dan gambar yang dideskripsikan sehingga lebih mudah dibaca dan dipahami. Hasil penelitian menunjukkan bahwa Lernaea yang berhasil diidentifikasi adalah L. cyprinacea, L. oryzophila dan L. osphronemi. Spesies-spesies tersebut diidentifikasi berdasarkan bentuk holdfast, kaki renang, uropoda, kantung telur dan panjang tubuh. Pengamatan dilakukan menggunakan mikroskop dengan perbesaran 100 X kemudian digambar dengan skala 2,5 cm. Predileksi Lernaea yang menyerang ikan gurami adalah pada permukaan tubuh, sirip, insang dan mata. Namun, di Desa Ngrajek Lernaea banyak menyerang di daerah sirip karena bagian sirip merupakan bagian yang memiliki jaringan yang tipis sehingga Lernaea mudah menancap.

Identification of Ectoparasites in Pearl Catfish (Clarias gariepinus) One and Three Months Ages in Makalele Cultivation Tuban District, Tuban Regency Galuh Swa Ardhya; Azizatun Nimah; Hani Plumeriastuti; Setiawan Koesdarto; Budiarto Budiarto
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

ABSTRACTÂ Â Â Â Â Â Â Â Â Â Â Â The purpose of this study was to identify ectoparasites that attack pearl catfish and the incidence of ectoparasite infestation in 100 samples of pearl catfish aged one and three months. This research was carried out from January to March 2021 in Makalele's cultivation, Tuban's District, Tuban's Regency. The method used in this research is skin scraping and gill installation. Data analysis using Chi-Square Test with SPSS version 24.0 for windows. The results showed that there were 3 types of ectoparasites found, Trichodina nobilis, Trichodina pediculus, Chilodonella sp., Dactylogyrus sp. and the highest incidence of ectoparasites occurred in young fish compared to old fish with a percentage of 35% and 25%, respectively, and based on the chi-square test there was no significant difference (pï¸â‰¥0.05).Â Keywords: Clarias gariepinus, infestation level, ectoparasites.

Ovicidal Effects of Pineapple Peel Juice (Ananas comosus L.) as a Control of Ascaridians in Chicken (Gallus domesticus) In Vitro Indira Vania Carisya; Setiawan Koesdarto; Tjuk Imam Restiadi; Nove Hidajati
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research was to know whether pineapple peel juice can be used as an ovicidal of in vitro A galli. This study used eggs of A. galli worms obtained by collecting adult A. galli worms from the small intestines of fresh local chickens purchased from traditional markets in Surabaya. The research design used was a completely randomized design (CRD) with four treatments , the concentration of pineapple peel juice was 0% (Control), 5%, 10%, and 20%. Each treatment consisted of six replications, with observations on days 5, 10, 15 and 20. Developed eggs were identified with the presence of larvae within egg and the movement of larvae. The data obtained will be analyzed with the ANOVA Statistical Test with the SPSS device and then followed by the Duncan test. The results of statistical analysis showed that pineapple peel juice had a significant effect on the development of A. galli worm eggs In Vitro. The number of damaged A.galli eggs increased as they were given higher pineapple juice concentration. This proves that pineapple peel juice has higher anthelmintic effect with increasing concentration given. Pineapple peel juice 20% is the best juice concentration.

Identification of Ectoparasites and Gastrointestinal Tract Endoparasites in Stray Cats at Traditional Market of Bojonegoro City Karina Rachmawati; Setiawan Koesdarto; Iwan Sahrial Hamid; Dian Ayu Permatasari
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

ABSTRACT This study aims to determine the ectoparasites and endoparasites on gastrointestinal tract that infest stray cats in Bojonegoro traditional market. The samples in this study were ectoparasites and feces from 40 stray cats in Bojonegoro traditional market. The ectoparasite samples were examined using the permanent attachment method without staining, while the feces samples were examined using three methods, namely native, simple sedimentation and fulleborn floatation. A positive sample is considered if a parasite is found that matches the characteristics of a parasite in cats sourced from scientific references. The results of the examination of 40 stray cats found 27 positive ectoparasites, 23 positive worms and 6 positive protozoa. The types of ectoparasites found were Ctneocephalides felis (67.5%), Otodectes cynotis (15%) where age and gender did not affect the prevalence, while the endoparasites found were Toxocara cati (42.5%), Ancylostoma sp. (22.5%) and Isospora felis (15%), age has effect on the prevalence obtained and sex has no effect . Keywords : Stray cats, Ectoparasites, Endoparasites in gastrointestinal tract

Identification Prevalence Of Endoparasite In Canines Bayan Lepas, Penang Rekasni Adallin A/P Morgan A/P Morgan; Setiawan Koesdarto; Maslichah Mafruchati; Mufasirin; Adiana Mutamsari Witaningrum
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.44132

This study aims to determine the type, prevalence and influence of sex and age on the prevalence of gastrointestinal parasites on dogs in Bayan Lepas, Penang. A total of 100 samples of dog feces were collected from three Districts. Feces examination was performed by direct smear, sedimentation, and floatation methods. The kind of helminth was Trichuris sp. 2% (2/100), Dipylidium sp. 2% (2/100), Ancylostoma sp. 1% (1/100), Toxocara sp. 2% (2/100), Strongyloides sp. 2% (2/100) and protozoa Isospora sp. 18% (18/100), Giardia sp. 1% (1/100) samples were positively infected by helminth and protozoa. According to the statistics analysis, could be concluded that sex and age do not affect the prevalence of gastrointestinal parasite.

Detection Of Parasitic Helminth In Gabus Fish (Channa Striata) Natural Catches At Baduk Fish Market Nganjuk Regency Anita Nur Aida Aida; Setiawan Koesdarto; Emy Koestanti Sabdoningrum; Dian Ayu Permatasari
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.44287

This study aimed to detect the existence and identify, also to determine the prevalence and degree of infection from parasitic helminth in gabus fish (Channa striata) natural catches at Baduk Fish Market, Nganjuk Regency. In 50 samples of gabus fish, parasitic helminths were examined on the body surface, gills, abdominal cavity, muscles, and digestive tract then identified. Parasitic helminths from digestive tract stained with Semichen Acetic Carmine method. Parasitic helminths that have been identified then calculate the prevalence and degree of infection. The result showed that the parasitic helminths found in gabus fish consist of the genera Pallisentis, Spinitectus, Dactylogyrus, and Gyrodactylus. Pallisentis sp. included in Phylum Acanthocephala, found to infect the intestines with prevalence of 74% and degree of infection of 5,62 parasite each fish. Spinitectus sp. included in Phylum Nematoda, found to infect the stomach and intestines with prevalence of 48% and degree of infection of 8 parasite each fish. Dactylogyrus sp. included in Class Monogenea, found to infect the gills with prevalence of 80% and degree of infection of 1,95 parasite each fish. Gyrodactylus sp. included in Class Monogenea, found to infect the body surface with prevalence of 42% and infection rate of 1,33 parasite each fish.

CHARACTERIZATION OF VirB4 PROTEIN OF LOCAL ISOLATE Brucella abortus WITH WESTERN BLOTTING TECHNIQUE Ratih Novita Praja; Didik Handijatno; Setiawan Koesdarto; Aditya Yudhana
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

This research aimed to characterize VirB4 protein of local isolate Brucella abortus with Western blotting method. The result showed that there were four protein bands with molecular weights of 64.61, 59.25, 21.63, and 16.70 kDa by triggering a reaction between the whole Brucella abortus and anti-Brucella abortus serum. The results also revealed that there was only one protein band with a molecular weight of 59.25 kDa triggering a reaction between the whole Brucella abortus and anti-VirB Brucella abortus serum. Finally, it can be concluded that VirB4 protein can affect the virulence factor of Brucella abortus, successfully characterized with the appearance of one band with a molecular weight of 59.25 kDa by using Western blotting method.

Identification and Prevalence of Endoparasite on Layer Chicken in Udanawu Sub-district Blitar Khalissa Farah Alifia; Setiawan Koesdarto; Yulianna Puspitasari; Mufasirin; Adiana Mutamsari Witaningrum
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.38769

The aim of this research was to determined the prevalence and infection degrees of endoparasite on layer chicken in Sub-district Udanawu, Blitar. Ninety-six samples were taken from layer chicken in 3 different villages namely Bakung Village, Tunjung Village, and Slemanan Village. The examinations taken in this study are fecal examination using native, sediment, and floating methods and blood examination using blood smear method. Result showed that 81.25% samples are positive for helminthiasis infection consisting of Ascaridia galli (66.67%), Heterakis gallinarum (45.83%), Raillietina sp. (31.25%), and Strongyloides avium (7.29%). Blood examination result shown there is no positive sample that infect layer chicken in Sub-district Udanawu, Blitar. Chi-Square test result showed there are significant difference (P<0.05) of Ascaridia galli and Heterakis gallinarum in Bakung Village, Tunjung Village, and Slemanan Village in Sub-district Udanawu, meanwhile there are no significant difference (P>0.05) of Raillietina sp. and Strongyloides avium. Range of infection degrees of helminthiasis in Bakung village, Tunjung village, and Slemanan Village are 608.75 Â± 588.53, 223.12 Â± 359.21, 156.25 Â± 332.39. There are significant difference (P<0.05) on helminthiasis infection degree of layer chicken in Udanawu District, Blitar.

Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Muhammad Rofi' Prasetya; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Kusnoto Kusnoto; Muchammad Yunus
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18201

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 Âµm and a ventral sucker (VS) with a diameter of 571 Âµm. It was also found the anterior spine with a width of 25.4 Âµm and a height of 22.8 Âµm. In the midbody, the spine has a width of Â± 26.7 Âµm and a length of Â± 38.9 Âµm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.

Co-Authors Adiacahya, Eren Adiana Mutamsari Witaningrum Agnes Theresia Soelih Estoepangestie Agus Wijaya Akbar Haryo Widhi Trismiharto Aksono HP., Eduardus Bimo Aldi Hamdani Amelia Dwita Safitri Ana Amaliah Anita Nur Aida Aida Annisa Novia Damayanti APRIL HARI WARDHANA Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azizatun Nimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Boedi Setiawan Bryan Ahmad Affan Lubis BUDI UTOMO Budiarto Budiarto Budiarto Budiarto Dadik Rahardjo, Dadik Dewi Mariyam Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Emy Koestanti Sabdoningrum Endang Suprihati Eny Coolfina Simarmata Felita Widyaningsih Firman Hadi Fanani Galuh Swa Ardhya Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Ida Yuniarti Indah Norma Triana, Indah Norma Indira Vania Carisya Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid jayawardhana, andi Jessica Giovani Karina Rachmawati Khairullah, Aswin Rafif Khairunnisa, Hanifa Khansa Khalissa Farah Alifia Kismiyati , Koesnoto Supranianondo Koesnoto Supriandono Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurnianto, Muhammad Aqil Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Mafruchati, Maslichah Mas’ud Hariadi Melani Anggraini Melanie Aulia Ashfiyah Meles, Dewa Ketut Mirni Lamid Mirny Lamid Mochamad Lazuardi Mohamad Safri Sauqi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Nenny Harijani Ngakan Made Rai Widjaja Nidom, Chairul Anwar Nila Murodah Nisa’ Rachmaningtyas Putri Nove Hidajati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurhusien Yimer Ogen Sea Poedji Hastutiek Poetranto, Emmanuel Djoko Praja, Ratih Novita Prasetya, Muhammad Rofi' Pratiwi, Arif Primarizky, Hardany Pudji Srianto Putri, Valerie Brilianda Rahadju Ernawati Rahaju Ernawati Rahardjo, Adi Prijo Rahmi Sugihartuti Rari Noviana Ratih Prajnya Paramitha Rekasni Adallin A/P Morgan A/P Morgan Rimayanti Rimayanti Risky Aprillian, Risky Rochmah Kurniasanti Santosa, Gusti Ayu Illiyin Putri Sari, Aulia Puspita Sari, Putri Desi Wulan Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawati Sigit Soeharsono Soeharsono Sofie Adi, Keren Jayanti Sri Agus Sudjarwo Sri Mumpuni Sosiawati Sri Mumpuni Sosiawati Sunarso, Agus Suwarno Suwarno Suzanita Utama Syah Putra, Akyun Rozaqi Tanjung, Aldi Gusnizar Rizaldy Tita Damayanti Lestari Tjuk Imam Restiadi Trifena Pristi Anindyta Tyasningsih, Wiwiek Tyasningsih, Wiwik Vanna Lidya Kharisma Vindo Rossy Pertiwi Virgy Dzakina Fahrezy Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla

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