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All Journal Jurnal Sain Veteriner Jurnal Veteriner Indonesian Journal of Tropical and Infectious Disease Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Jurnal Medik Veteriner Journal of Aquaculture and Fish Health Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary Jurnal Kedokteran Hewan Journal of Fisheries & Marine Jurnal Agro Veteriner

Setiawan Koesdarto

Departemen Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga

Author-ID : 515120

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Microscophy Identification of Toxocara cati First Stage Larvae and Second Stage Larvae Eny Coolfina Simarmata; Kusnoto Kusnoto; Mochamad Lazuardi; Setiawan Koesdarto; Endang Suprihati; Kuncoro Puguh Santoso
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study was aimed to identify the ultra structural morphology of Toxocara cati First Stage Larvae and Second Stage Larvae using Light Microscope. Toxocara cati larvae were obtained from adult worm eggs then were treated in phospat buffer saline with comparasion 1 : 1 until it reached the stage of larvae 1 and 2. The shell of egg Toxocara cati were thick and usually consist of three layers. The first layer was inner membrane, the second layer was middle layer and the third was outer layer. The results of larvae morphology that were identified using light microscope showed that the anterior part of Toxocara cati first stage larvae has a dorsal lip and esophagus and intestine on the posterior part but it could not be identified perfectly. The anterior part of Toxocara cati second stage larvae that were identified has three lips that leads directly into the oesophagus. Three lips on the anterior part of Toxocara cati larvae 2 consist of a dorsal lip and two subventral lips. Morphological of Toxocara cati larvae similar with the Toxocara cati adult worms because morphology of larvae and adult stage was difficult to differentiated.

Prevalence of Blood Protozoa Disease on Cattle and Buffalo in Moyo Hilir Sub-District, Sumbawa District West Nusa Tenggara Melani Anggraini; Hardany Primarizky; Mufasirin Mufasirin; Lucia Tri Suwanti; Poedji Hastutiek; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research was to determine the type and prevalence of blood protozoa disease on cattle and buffalo in Moyo Hilir sub-district, Sumbawa District, Wes Nusa Tenggara. This research was taken on july until December 2018. This research used a non-experimental method through an observation study. The samples of this research were made in thin blood smear using 20% Giemsa’s stain and all samples were from 200 blood of cattle and buffalo in Moyo Hilir sub-district, Sumbawa District, West Nusa Tenggara. The results showed that the prevalence of blood protozoa disease was 11.5 Based on the type of blood protozoa, the highest prevalence was Anaplasma sp. (11%), followed by Theileria sp. (0.5%), while Babesia sp. And Trypanosoma sp. Were not found. Based on the livestock types, the prevalence in buffalo was higher (17%)than cttle (6%). Based on sex the prevalence in female was higher (12.6%) than male (10.11%). Based on age of livestock, the prevalance of livestock with age > 2 years old was higher (13.33%) than livestock with age ≤ 2 years old (10.4%) based on the location of the village, blood protozoa disease were only found in Olatrawa village (17.85%) and Serading village (17.82%), while in Kakiang and Moyo village were not found. The conclusion of this research indicate that prevalence of blood protozoa disease was 11.5% and only Anaplasma sp. And Theileria sp. Were found.

The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro Amelia Dwita Safitri; Iwan Sahrial Hamid; Poedji Hastutiek; Setiawan Koesdarto; Rahmi Sugihartuti; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design. There were five treatments of physiological NaCl solution (K-), piperazine sitrate (K+), etanol extract of bitter leaf 0,35% (P1), etanol extract of bitter leaf 1,4% (P2), etanol extract of bitter leaf 4,2% (P3), and each treatment was done in four replications. This research used ten Ascaridia galli in each treatment for all replications. The observation and recording of dead Ascaridia galli was done at 2, 4, 6, 8, 10 and 12 hours. Ascaridia galli were declared dead if there was no movement when disturbed by anatomy tweezer and when dipped in slightly warm water (50°C). The obtained data was analyzed using ANAVA and continued with Duncan Multiple Range Test. The result of this research show that etanol extract of bitter leaf (Vernonia amygdalina) has anthelmintic effects against Ascaridia galli worm in Vitro. In the extract with 4,2% concentration, there is anthelmintic property that almost the same as Piperazine sitrate 10 mg/ml. the higher the concentration of extract, the higher the property of anthelmintic. In probit analysis show that EC50 achieved by concentration 2.093% with the low concentration of .002% and the highest concentration of 3.632%. LT50 of ethanol extract of bitter leaf was 0.35% at 10.323 hours, 1.4% at 9.800 hours, 4.2% at 7.864 hours and Piperazine sitrate 10 mg/ml at 9.013 hours.

Prevalence and Intensity of Nematode Infection on The Crossbreed Horse in Detasemen Kaveleri Berkuda Parongpong Bandung West Java Sesa Puput Febriyanti; Lucia Tri Suwanti; Eka Pramyrtha Hestinah; Setiawan Koesdarto; Boedi Setiawan; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This survey study was aimed to determine the prevalance of nematodosis and intensity on the Crossbreed Horse in Detasemen Kaveleri Berkuda Parongpong Bandung West Java. The benefits of this research are to contribute giving additional information about Prevalence, Intensity, and Relation among prevalance with age, sex, and stable. Fecal samples were tested by native, sedimentation, and floatation methods. The examination was found some kind of worm eggs, which are: Trychonema sp., Strongylus spp., Dictyocaulus arnfieldi (lung worm). The result showed that the prevalence of nematodosis was 12.03%. in the calculation of worm eggs per gram feces of Crossbreed Horse in Detasemen Kaveleri Berkuda that have relatively moderate infection were 1123EPG and result analyze with ANOVA showed no real different with function of horse. The result of statistical analysis using Chi-Square test showed no significant differences in prevalence with age and sex (p>0.05). the result of statistical using Chi-Square test showed significant differences in prevalence with stable in Detasemen Kaveleri Berkuda (p<0.05).

Prevalence of Small Intestine Cestodes in Goat at Pegirian Slaughterhouse Surabaya Bryan Ahmad Affan Lubis; Setiawan Koesdarto; Eka Pramyrtha Hestinah; Kusnoto Kusnoto; Lucia Tri Suwanti
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study was conducted to determine the prevalence of small Intestine cestodes in goat at Pegirian Slaughterhouse Surabaya with gastrointestinal dissection method. Thirty-six samples of goatâ€™s small intestine, twenty-five from Kacang goats and eleven from Ettawa-cross (PE) goats, were collected from Pegirian Slaughterhouse Surabaya on May 2018. Fresh adult worms were collected with gastrointestinal dissection and microscopically identified. The result showed that cestodes infections could be identified in three (12%) of Kacang goats and three (27.27%) of PE goats. The cestode infections were identified five positive samples (19.23%) in male goat and one positive sample (10%) in female goat, while determined by the ages found three positive samples (17.64%) in under two years old goats and three positive samples (15.78%) in more than or equal two years old goats. The Chi-Square statistical test (p >0.05) showed there was no significant correlation from the race, sex, and ages of goats on the prevalence of Moniezia expansa.

The Distribution of Goat Gastrointestinal Tractus Worm Egg at Rambon District of Nganjuk Regency Arum Puspitasari; Boedi Setiawan; Setiawan Koesdarto; Kusnoto Kusnoto; Soeharsono Soeharsono; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the distribution of worm tract infection in foat digestion in Sub-district of Prambon, Nganjuk Regency. This research was conducted in Agustus 2018-Januari 2019 with 108 samples of stool examination iin laboratory of Helmintologi Airlangga University Department of Parasitology, were examinated by native, sedimentation. Floatation techniques, and distribution of worm types of feces. The results showed that 66 samples were positive infected by gastrointestinal worm, its indicated that helminthiasis was 61,11%. On examination it was found some kind of worm eggs, which are: Oesophagustomum spp., Bunostomum spp., Haemonchus spp., Trichostrongylus spp., Strongyloides spp., Trichuris spp., Chabertia spp and Moniezia benedini. The results of study showed the most of worm distribution in feces is Haemonchus spp.

Identification of Blood Parasite on Sacrifical Cattle Slaughtered during Idul Adha 1438 H in Surabaya City and Sidoarjo Regency Dhimar Maulud Dyahningrum; Mufasirin Mufasirin; Nenny Harijani; Poedji Hastutiek; Setiawan Koesdarto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research is to detect the presence of blood parasite that infects sacrificial cattle slaughtered during idul adha 1438 H in Surabaya City and Sidoarjo Regency. This research used 147 blood samples of sacrificial cattle and used two methods, those are thin blood smear stained with Giemsa 20% and Microhematocrit Centrifugation Technique. Based on the result of the examination using a microscope with 1000x magnification, there are positive samples infected with blood parasite, that consist of Babesia sp., and Anaplasma sp.

Prevalence of Rumen and Reticulum Trematodes in The Goat Slaughtered at Pegirian Surabaya Slaughter House using Digestive Surgery Method Novia Intan Kurnia; Setiawan Koesdarto; Herry Agoes Hermadi; Kusnoto Kusnoto; Hardany Primarizky; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to determine the kind of trematodes which infected the rumen and reticulum of goat slaughtered at RPH Pegirian Surabaya using digestive surgery method, and determine the prevalence result. 36 samples of rumen and reticulum were collected from RPH Pegirian from September - November 2017 then using digestive surgery to obtain fresh adult worms, afterwards using the Semichen-Acetic Carmine staining method to makes the object easy to identifying. The results showed that species was found on goat was Paramphistomum cervi and Cotylophoron cotylophorum, in the Paramphistomum cervi are several characters wich is oral sucker, genital pore, uterus, testis, ventral sucker and in Cotylophoron cotylophorum contain oral sucker, branched oesophagus, uterus, vitelin glands, testes , posterior sucker. 3 rumen positive trematodosis with prevalence of 8.33% Chi-square statistic test (p>0.05) showed no effect between goat species and Trematodosis prevalence, 20 kacang goat and 16 peranakan etawa goat found 2 positive kacang goat (10%) and 1 peranakan etawa goat positive (6.25%).

Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique Firman Hadi Fanani; Kusnoto Kusnoto; Poedji Hastutiek; Setiawan Koesdarto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 Î¼g/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa.

Efektifitas Anthelmintika Ekstrak Etanol Kulit Buah Delima (Punica granatum) terhadap Jumlah Kematian Cacing Ascaridia gallisecara inVitro Nila Murodah; Sri Mumpuni Sosiawati; Iwan Sahrial Hamid; Setiawan Koesdarto; Rochmah Kurniasanti; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

This research was conducted to determine the anthelmintics effect of peel a pomegranate fruit extract etanol against Ascaridia galli worm in vitro. In this research, using 240 samples of A. galli with length 7-11cm without differentiating sex. The concentration of peel a pomegranate fruit extract etanol for immersing the Ascaridia galli were 10 mg/ml, 20 mg/ml, 40 mg/ml, and 80 mg/ml. Negative control was used PBS. Positif control was used piperazin citrate 10 mg/ml. Observation death A. galli worm and analysis of the data at the 6 hour, 12 hour, 18 hour, 24 hour, 30 hour and 36 hour using ANOVA test and Duncan Range Test. ANOVA result showed significant differences between treatments (p<0,05). Duncan Range Test result showed peel a pomegranate extract etanol 40 mg/ml had anthelmintics effects the 36 hour which comparable with piperazin citrate 10 mg/ml.

Co-Authors Adiacahya, Eren Adiana Mutamsari Witaningrum Agus Wijaya Akbar Haryo Widhi Trismiharto Aksono HP., Eduardus Bimo Aldi Hamdani Amelia Dwita Safitri Ana Amaliah Anita Nur Aida Aida Annisa Novia Damayanti APRIL HARI WARDHANA Arum Puspitasari Aryaloka, Suhita Ayuti, Siti Rani Azizatun Nimah Bagaskara, Ryan Bambang Sektiari Lukiswanto Boedi Setiawan Bryan Ahmad Affan Lubis BUDI UTOMO Budiarto Budiarto Budiarto Budiarto Dadik Rahardjo, Dadik Dewi Mariyam Dhimar Maulud Dyahningrum Dian Ayu Permatasari Didik Handijatno Didik Handijatno Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Emy Koestanti Sabdoningrum Endang Suprihati Eny Coolfina Simarmata Felita Widyaningsih Firman Hadi Fanani Galuh Swa Ardhya Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hasib, Abdullah Heni Puspitasari Hermin Ratnani Herry Agoes Hermadi Ida Yuniarti Indah Norma Triana, Indah Norma Indira Vania Carisya Inggarsetya Syah Audini Ira Sari Yudaniayanti Iwan Sahrial Hamid Iwan Sahrial Hamid Iwan Sahrial Hamid jayawardhana, andi Jessica Giovani Karina Rachmawati Khairullah, Aswin Rafif Khairunnisa, Hanifa Khansa Khalissa Farah Alifia Kismiyati , Koesnoto Supranianondo Koesnoto Supriandono Kuncoro Puguh Santoso Kuncorojakti, Suryo Kurnianto, Muhammad Aqil Kurniawati, Dyah Ayu Kusnoto Kusnoto Kusnoto Kusnoto, Kusnoto Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Lutfiah Annisa Billa Mafruchati, Maslichah Mas’ud Hariadi Melani Anggraini Melanie Aulia Ashfiyah Meles, Dewa Ketut Mirni Lamid Mirny Lamid Mochamad Lazuardi Mohamad Safri Sauqi Moses, Ikechukwu Benjamin Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Mustofa Helmi Effendi Nenny Harijani Ngakan Made Rai Widjaja Nidom, Chairul Anwar Nila Murodah Nisa’ Rachmaningtyas Putri Nove Hidajati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurhusien Yimer Ogen Sea Poedji Hastutiek Poetranto, Emmanuel Djoko Praja, Ratih Novita Prasetya, Muhammad Rofi' Pratiwi, Arif Primarizky, Hardany Pudji Srianto Putri, Valerie Brilianda Rahadju Ernawati Rahaju Ernawati Rahardjo, Adi Prijo Rahmi Sugihartuti Rari Noviana Ratih Prajnya Paramitha Rekasni Adallin A/P Morgan A/P Morgan Rimayanti Rimayanti Risky Aprillian, Risky Rochmah Kurniasanti Santosa, Gusti Ayu Illiyin Putri Sari, Aulia Puspita Sari, Putri Desi Wulan Sarmanu, Sarmanu Septian Hakim Susantoputro Sesa Puput Febriyanti Setiawati Sigit Soeharsono Soeharsono Soelih Estoepangestie, Agnes Theresia Sofie Adi, Keren Jayanti Sri Agus Sudjarwo Sri Mumpuni Sosiawati Sri Mumpuni Sosiawati Sunarso, Agus Suwarno Suwarno Suzanita Utama Syah Putra, Akyun Rozaqi Tanjung, Aldi Gusnizar Rizaldy Tita Damayanti Lestari Tjuk Imam Restiadi Trifena Pristi Anindyta Tyasningsih, Wiwiek Tyasningsih, Wiwik Vanna Lidya Kharisma Vindo Rossy Pertiwi Virgy Dzakina Fahrezy Warsito, Sunaryo Hadi Wibawati, Prima Ayu Widya Paramita Lokapirnasari Yeni Dhamayanti Yudhana, Aditya Yulianna Puspitasari Yunus, Muchammad Yunus, Muchammad Zhaza Afililla

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