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Platelet-to-Lymphocyte Ratio in Pediatric Dengue Patients: A Key Indicator of Disease Severity Silma Farraha; Husni; Deswita Sari; Rikarni; Zelly Dia; Dwi Yulia
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 5 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i5.1278

Abstract

Background: Dengue infection remains a significant health concern in Indonesia, with a high mortality rate. Early identification and prediction of severe dengue are crucial for effective management and mortality reduction. The platelet-to-lymphocyte ratio (PLR) has emerged as a potential biomarker for assessing dengue severity due to its association with inflammatory responses. Methods: This descriptive study included 48 pediatric patients diagnosed with dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) at Dr. M. Djamil General Hospital Padang between March and August 2024. Patients were included if they were aged 0-18 years, had a confirmed diagnosis of DHF or DSS, and underwent complete blood count and serological testing for dengue. Patients with chronic diseases or other comorbidities were excluded. Complete blood counts were performed using flow cytometry, and PLR was calculated by dividing the platelet count by the absolute lymphocyte count. Clinical data were obtained from medical records. Results: The majority of patients were aged 6-18 years (68.8%), with 20 (41.7%) presenting with DHF and 28 (58.3%) with DSS. The median platelet count was lower in DSS patients (26,000/mm3) compared to DHF patients (35,500/mm3). The median PLR was also significantly lower in DSS patients (8.95) compared to DHF patients (15.61). A PLR value <20 was more frequently observed in DSS patients (89.3%) than in DHF patients (75%). Conclusion: A lower PLR value was associated with more severe clinical manifestations of dengue infection, particularly DSS. PLR can serve as a valuable biomarker for assessing dengue severity, utilizing readily available and cost-effective complete blood count results.
The Triglyceride-Glucose Index: A Potential Simple Screening Tool for Insulin Resistance in Young Adults Kenny; Efrida; Elfira Yusri; Rismawati Yaswir; Husni; Syofiati
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 5 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i5.1281

Abstract

Background: Insulin resistance is a key factor in the development of metabolic diseases, which are increasingly prevalent in young adults. Early detection is crucial for timely intervention. The triglyceride-glucose (TyG) index, calculated from fasting triglyceride and glucose levels, has emerged as a potential alternative to the more complex homeostasis model assessment of insulin resistance (HOMA-IR). This study aimed to evaluate the agreement between the TyG index and HOMA-IR in detecting insulin resistance in young adults. Methods: A cross-sectional study was conducted on 102 non-diabetic young adults (aged 18-22 years). Fasting blood samples were collected to measure triglyceride, glucose, and insulin levels. The agreement between the TyG index and HOMA-IR was assessed using the Kappa coefficient. Results: The median age of the participants was 20 years, with 65.7% being female. The prevalence of insulin resistance was 79.4% based on the TyG index (cut-off value of 4.25) and 43.1% based on HOMA-IR (cut-off value of 2.2). The agreement between the two indices was slight (Kappa = 0.155, p = 0.001). Conclusion: The TyG index showed a higher prevalence of insulin resistance compared to HOMA-IR in this population. However, the agreement between the two indices was low. Further research is needed to validate the TyG index as a screening tool for insulin resistance in young adults.
Impact of Donation Frequency on Iron Stores and Hemoglobin Levels in Regular Blood Donors Lili Novri Yanti; Rofinda, Zelly Dia; Syofiati; Rikarni; Husni; Elfira Yusri
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 5 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i5.1282

Abstract

Background: Regular blood donation, while crucial for healthcare systems, can lead to iron deficiency and anemia in donors. This study investigated the impact of donation frequency on iron stores and hemoglobin levels in regular blood donors. Methods: A cross-sectional study was conducted on 82 regular blood donors at the blood transfusion unit of Dr. M. Djamil General Hospital from February to April 2024. Participants underwent pre-transfusion screening, including a questionnaire on donation frequency and iron supplement use. Blood samples were collected during donation, and serum ferritin, hemoglobin, and erythrocyte indices (MCV, MCH, MCHC) were measured if the C-reactive protein (CRP) test was negative. Data were analyzed using the Kruskal-Wallis test. Results: The mean age of the donors was 31 years, with the majority being female (56.1%). The most common donation frequency was 6-10 times (34.15%). None of the donors reported using iron supplements. Serum ferritin levels showed significant differences among female donors based on donation frequency (p=0.004) but not among male donors (p=0.114). Hemoglobin levels also differed significantly among female donors (p=0.002), but not among male donors (p=0.213). Significant differences were observed in MCV and MCH values in both male and female donors (p<0.001 and p=0.001, respectively), but not in MCHC values (p=0.135). Conclusion: Donation frequency significantly impacts iron stores and hemoglobin levels in female blood donors but not in male donors. Regular monitoring of iron stores, particularly in female donors, is crucial to prevent iron deficiency and anemia.
The Clinical Significance of DAT Positivity: A Comparative Analysis of IgG, C3d, and IgG/C3d-Positive Patients Khaulah Karimah; Zelly Dia Rofinda; Husni; Rikarni; Deswita Sari; Yoshie Anto Chicamy
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 5 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i5.1288

Abstract

Background: The direct antiglobulin test (DAT) is a crucial diagnostic tool in immunohematology, used to detect the presence of antibodies and/or complement components on the surface of red blood cells. DAT positivity is frequently associated with autoimmune hemolytic anemia (AIHA) and other immune-mediated hemolytic conditions. This study aimed to investigate the clinical significance of DAT positivity by comparing the characteristics of patients with IgG, C3d, and IgG/C3d-positive results. Methods: A retrospective study was conducted on 55 patients with DAT-positive results, identified from the Blood Transfusion Unit of Dr. M. Djamil General Hospital Padang between June 2023 and August 2023. DAT-positive samples were further analyzed using monospecific anti-human globulin (AHG) reagents to determine the presence of IgG, C3d, or both on the red blood cells. Patient demographics, clinical diagnoses, blood groups, transfusion history, and hematological parameters were collected and analyzed. Results: Out of the 55 DAT-positive patients, 51 (92.7%) were positive for IgG alone, 3 (5.5%) were positive for both IgG and C3d, and only 1 (1.8%) was positive for C3d alone. The majority of patients were adults (>18 years old) and female. A history of blood transfusion (>3 times) was common, particularly in the IgG-positive group. Hematological parameters indicative of hemolysis (hemoglobin ≤9 g/dL, reticulocyte count >2%, and total bilirubin >2 mg/dL) were observed in a significant proportion of patients, especially those with IgG and/or C3d positivity. Conclusion: IgG positivity was the most common finding in DAT-positive patients, highlighting the prevalence of warm AIHA. The presence of C3d, alone or with IgG, suggests the involvement of complement activation and may indicate a different underlying pathology. This study emphasizes the importance of using monospecific AHG reagents to characterize DAT-positive results, as this information can aid in the diagnosis, management, and prediction of clinical outcomes.
Immunophenotyping vs. Bone Marrow Aspiration in Pediatric Acute Leukemia: A Comparative Analysis Satria Utomo; Yulia, Dwi; Elfira Yusri; Rismawati Yaswir; Husni; Desywar
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 9 No. 5 (2025): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v9i5.1289

Abstract

Background: Acute leukemia is a significant global health concern, with increasing prevalence worldwide and in Indonesia. Accurate diagnosis and classification of acute leukemia subtypes, primarily acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML), are crucial for effective treatment. Immunophenotyping and bone marrow aspiration (BMA) are commonly used diagnostic methods, each with its strengths and limitations. This study aimed to analyze the concordance between immunophenotyping and BMA in diagnosing and classifying acute leukemia in children. Methods: A retrospective cross-sectional study was conducted on 46 children diagnosed with acute leukemia at Dr. M. Djamil General Hospital Padang from January 2022 to July 2023. Data were collected from medical records, including patient demographics, immunophenotyping results, and BMA findings. Concordance between the two diagnostic methods was analyzed using Fisher's exact test. Results: The study population consisted of 30 (65.2%) males and 16 (34.8%) females, with a median age of 4 years. Immunophenotyping identified 24 (52.2%) cases as ALL and 22 (47.8%) as AML. BMA classified 26 (56.5%) cases as ALL and 20 (43.5%) as AML. There was a high concordance between the two methods, with only 2 (4.3%) cases showing discordant results. These two cases were classified as AML by immunophenotyping but as ALL by BMA. Conclusion: Immunophenotyping and BMA demonstrate a high level of concordance in diagnosing and classifying acute leukemia in children. The few discordant cases highlight the importance of considering both methods, especially in challenging cases, to ensure accurate diagnosis and appropriate treatment.
PENGARUH TEKNIK RELAKSASI NAFAS DALAM DAN AROMATERAPI KOPI TERHADAP KECEMASAN PADA PASIEN PRE-OPERASI LAPARATOMI Afiqah Jumatul Saadah; Septiyanti; Husni; Asmawati; Sariman Pardosi
Mitra Raflesia (Journal of Health Science) Vol. 17 No. 1 (2025)
Publisher : LPPM STIKES BHAKTI HUSADA BENGKULU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51712/m3hbdv08

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ABSTRAK Latar Belakang: Kecemasan merupakan keluhan subjektif yang dapat berdampak pada kebutuhan fisik dan psikologis serta akan dapat menyebabkan penundaan operasi pada pasien laparatomi. Teknik relaksasi nafas dalam dan aromaterapi kopi merupakan salah satu terapi yang dapat meningkatkan kenyamanan dan mengurangi ketegangan fisiologis maupun psikologis pada pasien pre-operasi laparatomi. Tujuan penelitian ini adalah untuk mengetahui pengaruh teknik relaksasi nafas dalam dan aromaterapi kopi terhadap penurunan kecemasan pasien pre-operasi laparatomi di RSUD Dr.M.Yunus Bengkulu. Metode: Jenis penelitian ini adalah pre-experimental mengunakan one grup pre-post test. Sampel penelitian adalah pasien pre-operasi laparatomi berjumlah 34 responden yang diambil mengunakan teknik purposive sampling. Perlakuan yang diberikan adalah intervensi relaksasi nafas dalam dan araomaterapi kopi yang dilakukan pada periode 6-24 jam sebelum operasi. Pengukuran kecemasan sesudah intervensi dilakukan 2 jam sebelum pasien masuk ke kamar operasi. Analisis data mengunakan uji Wilcoxon test. Hasil: penelitian menunjukkan ada penurunan skor kecemasan sebesar 14 poin setelah dilakukan intervensi. Kesimpulan:Ada pengaruh pemberian terapi teknik relaksasi napas dalam dan aromaterapi kopi terhadap kecemasan pada pasien pre-operasi laparatomi di RSUD Dr.M.Yunus Bengkulu (p- value 0,000 ? ? 0,05) Penerapan teknik relaksasi nafas dalam dan aromaterapi kopi sangat dianjurkan untuk menurunkan kecemasan pasien pre-operasi laparatomi. Kata kunci : aromaterapi ; kecemasan; relaksasi nafas dalam   ABSTRACT Background: Anxiety is a subjective complaint that can affect physical and psychological needs and can cause delays in surgery in laparotomy patients. Deep breathing relaxation techniques and coffee aromatherapy can increase comfort and reduce physiological and psychological tension in pre-operative laparotomy patients. The purpose of this study was to determine the effect of deep breathing relaxation techniques and coffee aromatherapy on reducing anxiety in pre-laparotomy patients at Dr. M. Yunus Regional Hospital, Bengkulu. Method: This type of research is pre-experimental using one group pre-post test. The research sample was 34 pre-operative laparotomy patients taken using a purposive sampling technique. The treatments given were deep breathing relaxation intervention and coffee aromatherapy conducted in the period 6-24 hours before surgery. Measurement of anxiety after intervention was conducted 2 hours before the patient entered the operating room. Data analysis used the Wilcoxon test. Results: of the study showed a decrease in anxiety scores of 14 points after the intervention.  Conclusion: The is an effect of providing deep breathing relaxation technique therapy and coffee aromatherapy on anxiety in pre-laparotomy surgery patients at Dr. M. Yunus Hospital, Bengkulu (p-value 0.000 ? ? 0,05). The application of deep breathing relaxation techniques and coffee aromatherapy is highly recommended to reduce anxiety in pre-laparotomy surgery patients. Keywords: aromatherapy; anxiety; deep breathing relaxation
Penyuluhan Hukum Tindak Pidana Judi Online Terhadap Siswa di Sekolah Menegah Atas (SMA) Negeri 1 Matang Kuli Zulfan; Muhammad Hatta; Johari; Husni; Joelman Subaidi
Academica : Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 1 (2025): Academica : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Pusat Studi Sosial dan Humaniora [LPS2H]

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.15259110

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Saat ini marak terjadi dikalangan remaja khsusnya remaja yang masih dibangku sekolah melakukan tindak pidana judi online. Dampak negatif tindak pidana judi online terhadap siswa sangat berbahaya dan dapat merusak masa depan siswa. Oleh karena itu, dosen Fakultas Hukum Universitas Malikussaleh berinisiatif melaksanakan kegiatan penyuluhan tindak pidana judi online terhadap siswa Sekolah Menegah Atas (SMA) Negeri 1 Matang Kuli, Aceh Utara. Oleh karena itu, kegiatan penyuluhan hukum kepada siswa terhadap tindak pidana judi online sangat dibutuhkan secara berkelanjutan. Untuk mengatasi permasalahan tersebut, Dosen Fakultas Hukum Universitas Malikussaleh telah mengadakan penyuluhan hukum tentang tindak pidana judi online di Sekolah Menegah Atas (SMA) Negeri 1 Matang Kuli Kabupaten Aceh Utara. Setelah kegiatan penyuluhan hukum dilakukan, maka kesadaran siswa terhadap dampak negatif daripada judi online semakin meningkat, sehingga para siswa menghindari tidak tindak pidana judi online
Caregiver Support as a Predictor of Self-Care in Older Adult with Hypertension: A Cross-Sectional Analysis from West Sumatera Nursing Home Jepisa, Tomi; Syarli, Setiadi; Husni; Ahmaniyah
Journal of Health Sciences and Epidemiology Vol. 3 No. 1 (2025): April 2025
Publisher : RRZ Scientific Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62404/jhse.v3i1.79

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Hypertension is a prevalent non-communicable disease in older adults that significantly affects functional abilities and quality of life. Half of the older adults had hypertension in nursing homes in West Sumatera. Caregiver support plays a crucial role as an external factor that can strengthen the motivation and ability of older adults to manage their health conditions independently. Most studies have focused on home-based care or informal caregivers. Therefore, this study aimed to assess the relationship between caregivers’ social support and self-care behavior in older adults with hypertension in nursing homes. This cross-sectional study, conducted from April to August 2025 at Sabai Nan Aluih Sicincin Nursing Home in West Sumatera, included 86 older adults with hypertension (aged ≥60 years) using total sampling. Data were collected via the 20-item Caregiver Social Support Questionnaire and the 25-item Hypertension Blood Pressure Self-Care Profile (HBP-SCP), both culturally adapted and reliable. Data were analyzed using descriptive analysis and chi-square tests. Result: Participants (mean age 72.4 years) were mostly female (61.6%) and widowed (74.4%). High caregiver support was perceived by 53.5% of the participants. Good self-care was demonstrated by 43.0% of participants, with the highest adherence in medication use and the lowest in blood pressure monitoring and physical activity. A significant association was found between caregiver support and self-care behavior (p=0.005). Conclusion: This study confirmed a significant positive association between caregiver support and self-care in older adults with hypertension in West Sumatra nursing homes. This underscores the critical role of formal caregivers in institutional settings. The findings highlight the need for comprehensive caregiver training in technical skills, communication, and motivational strategies. Future longitudinal research is recommended to explore causality and develop targeted interventions. Fostering robust caregiver support is essential for promoting independence and improving the quality of life of these individuals.      
Penyuluhan Hukum Pentingnya Rambu Lalu Lintas Keselamatan di Jalan Terhadap Siswa di Sekolah Dasar Negeri 3 Matang Kuli Kabupaten Aceh Utara Muhibuddin; Fatahillah; Ummi Kalsum; Zul Akli; Zainal Abidin; Husni
Academica : Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 2 (2025): Academica : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Pusat Studi Sosial dan Humaniora [LPS2H]

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/zenodo.16416579

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Saat ini seing terjadi kecelakaan lalu lintas adalah faktor human (manusia), faktor kendaraan, faktor jalan (sarana prasarana) dan faktor cuaca. Faktor manusia menjadi faktor tertinggi sebagai penyebab kecelakaan lalu lintas. disebabkan faktor manusia karena, lengah, tidak tertib, tidak terampil, dan kecepatan tinggi. Kecelakaan paling banyak terjadi pada anak muda usia 18-23 tahun yaitu sebanyak 259 dari 851 kecelakaan di sepanjang tahun 2008-2010. Hal ini dapat dikarenakan pada rentang umur 18- 23 tahun merupakan kelompok umur yang memiliki mobilitas tinggi dengan berbagai aktifitas dan cenderung masih labil dalam berkendara karena usia yang muda. Maka dosen Fakultas Hukum Universitas Malikussaleh berinisiatif melaksanakan kegiatan penyuluhan Hukum Pentingnya Rambu Lalu Lintas Keselamatan di Jalan Terhadap Siswa di Sekolah Dasar Negeri 3 Matang Kuli Kabupaten Aceh Utara. Maka kegiatan penyuluhan Hukum Pentingnya Rambu Lalu Lintas Keselamatan di Jalan Terhadap Siswa di Sekolah Dasar Negeri 3 Matang Kuli Kabupaten Aceh Utara,Untuk mengatasi permasalahan tersebut, Dosen Fakultas Hukum Universitas Malikussaleh telah mengadakan penyuluhan Hukum Pentingnya Rambu Lalu Lintas Keselamatan di Jalan Terhadap Siswa di Sekolah Dasar Negeri 3 Matang Kuli Kabupaten Aceh Utara. Setelah kegiatan penyuluhan hukum dilakukan, maka kesadaran anak-anak sejak usia dini mengenai pentingnya keselamatan di jalan, merupakan cara untuk membentuk pola pikir dan karakter pada anak-anak sehingga diharapkan anak-anak menjadi disiplin dalam berlalu lintas dan dampaknya dapat menimalisir terjadinya kecelakaan dijalan raya.
Analisis Pengelolaan Keuangan Dana Desa di Desa Tangru Kecamatan Malua Kabupaten Enrekang Husni; Sahade; Azwar Anwar
Menulis: Jurnal Penelitian Nusantara Vol. 1 No. 7 (2025): Menulis - Juli
Publisher : PT. Padang Tekno Corp

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59435/menulis.v1i7.554

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Penelitian ini bertujuan untuk menganalisis pengelolaan keuangan dana desa di Desa Tangru Kecamatan Malua Kabupaten Enrekang serta mengidentifikasi faktor-faktor penghambat dalam pengelolaannya. Pengelolaan keuangan desa menjadi instrumen penting dalam upaya mewujudkan tata kelola pemerintahan desa yang akuntabel, transparan, dan partisipatif. Penelitian ini menggunakan pendekatan kualitatif deskriptif dengan teknik pengumpulan data berupa wawancara dan dokumentasi terhadap aparat desa dan dokumen APBDes tahun 2020. Hasil penelitian menunjukkan bahwa pengelolaan dana desa di Desa Tangru telah mengacu pada Permendagri Nomor 113 Tahun 2014 dan Permendagri Nomor 20 Tahun 2018, namun masih ditemukan beberapa hambatan, seperti keterlambatan transfer dana, ketidaksesuaian realisasi anggaran, serta keterlambatan dalam penutupan buku. Oleh karena itu, perlu peningkatan kapasitas sumber daya manusia serta penguatan pengawasan internal.
Co-Authors A. R. Jatnika Abdul Azis Abdul Majid, Basrul Ade Yeni Afiqah Jumatul Saadah Afriani, Nita Ahmaniyah Akhyar Ali, Masri ALI, NURDIN Arianto Syahruddin Arif, Zainal Asmawati Aulia Husniati Azwar Anwar Badriah Badriah Bastiar Bukhari Dandi Bahtiar Desiekawati Deswita Sari Desywar Dian Pertiwi Dwi Yulia Efrida Efrida Elfira Yusri Fatahillah Fatma Azzahra Fatma Azzahra Febryanto, Muhammad Desto Habibi Firman syah, Firman Fitriana, Maya Hartini Hendra Permana Hudaya, Chairul I. Munandar Ida Rahmah Burhan Ihsan, Rafiq Ilham Juliyannur Indrawati Noor Kamila IRMA DEWI ISKANDAR Jarnawi Jarnawi Jepisa, Tomi Johari Kenny Khairatun Hisan Khairil Fata Khaulah Karimah Kurniawan, Eka L. Abdullah Lili Novri Yanti Linda Wati M. Nizar Machmud Masri Ibrahim Mayviza, Liza Monarisa Muammar Muarif Setiawan Muhammad Amin Muhammad Fadillah Ghifari Muhammad Hatta Muhammad Nur Muhammad Nur MUHAMMAD RIZAL Muhammad Saleh Muhibbuddin Muhibuddin Mukhtasar Munawar Rizki Jailani Munawir Mustafa Nasrah Nasruddin Nasution, Elvy Sahnur Novita Laily Shalatiah Nurarafah Nurjannah Pardosi, Sariman Putra, T. Edisah R. Priyanto Ramalinggam Rajamanickam Reza , Mohamad Reza, T. Surya Rikarni Ririn Rismawati Yaswir Romi Asmara Roswati Ruliati, Ruliati S. Nealma Sabri Safrina, Lia Sahade Satria Utomo Septiyanti Silma Farraha Siregar, Askari Guna Siregar, Fadhilaturrizqie Siswahyudianto Siti Safaria Sofia Ratna Awaliyah Fitri Sofyan Jafar Subaidi, Joelman Sulaiman Thalib Sumiadi Syahrizal Syarli, Setiadi Syarwani Syofiati Tating Rohaeti Teuku Yudi Afrizal tomi jepisa Tomi Jepisa Ujang Endang Ujuh Ilham Simak Vania Ilmi Basri Wahid , Irza WAHYUNI Yoshie Anto Chicamy Yulia, Dwi Yulianti Yunengsih, Yuyun Yusuf Zahara, Annisa Zainal Abidin Zelly Dia Zelly Dia Rofinda Zikriatul Ulya Zul Akli Zulfadhli Zulfan Zulhilmi Zuliani, Rafiza