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A Novel Variant of HOXA10 gene, Ser19Cys, among Patients with Endometriosis and its Relationship with the Severity of the Disease Pinda Hutajulu; Djaswadi Dasuki; Ahmad Hamim Sadewa; Totok Utoro
Indonesian Journal of Biotechnology Vol 18, No 1 (2013)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.502 KB) | DOI: 10.22146/ijbiotech.7864

Abstract

Endometriosis is a gynecological disease associated with inherited genetic traits. HOXA10 gene whichis expressed in uterine plays an important role in the pathogenesis of endometriosis. The protein affects thedevelopment of pinopodes as a biomarker of endometrial receptivity in endometriosis.The aim of this study isto examine if there is a mutation or polymorphism within HOXA10 gene among patients with endometriosis.Thirty twopatients and 32 healthy women were recruited as subjects of this study. The exon 2 of HOXA10which covers most of coding region was amplifi ed using PCR. The presence of a mutation or polymorphismwas detected by direct seguencing. The distribution of genotype and allele was analyzed using Chi square test with p<0.05 is considered as signifi cantly different. A novel heterozygous variant within exon 2 of HOXA10 which substitute an adenine into thymine was detected at base position 55. This missense alteration changed amino acid serine to cystein (Ser19Cys). Interestingly, this variant was detected in 12 endometriosis cases (38%) but none in control. Patients carry HOXA10 Ser19Cys variant were associated with dismenorea and more frequent in stage I endometriosis. The role of this variant in the function of HOXA10 protein and frequency among Indonesians need to be clarifi ed. We found a novel heterozygous HOXA10 gene variant, Ser19Cys.The genotype frequency is 38% among endometriosis patients but none in control. This variant found in patient with dismenore and endometriosis stage 1. Key words: HOXA10 gene, endometriosis, Ser19Cys polymophism
The influence of endometriosis peritoneal fluid on the development of mouse embryo in in vitro pre-implantation O. Wahyu, Djaswadi Dasuki Hasto Wardoyo, Ita Fauziah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Endometriosis was associated with various immunologic changes detected both in the endometrial eutopic and blood edge sample. Some researchers reported that women with endometriosis have an increased amount of peritoneum fluid. Endometrial environment and peritoneal fluid diffusing to the ovarial tube may affect sperm-ovum interaction, initial phase of embryonic development and reduce endometrial receptability. The correlation between endometriosis and early in vitro embryonic development has been reported in some studies showing controversial results; therefore it is necessary to carry out researches to clarify the influence of endometriosis on the early embryonic development. Objective: to identify the influence of peritoneal fluid of infertile women with endometriosis and normal peritoneum fluid of fertile women on the development of mouse embryo in in-vitro pre-implantation. Methods: Prospective, randomized controlled trial subjects are 2 cells of the Swiss albino strain embryos super-ovulating procedure on mouse, 2 embryonic cells resulted from in-vivo fertilization were taken out and in-vitro culture was done with the exposure of 10% or 2.5% cell-free supernatant peritoneum fluid of infertile women with endometriosis and 10% or 2.5% cell-free supernatant peritoneum fluid of normal fertile women as the control. The blastocyst growth was observed in the 72 hour culture and hatching blastocyst culture in 96 hours. Results: An amount of 231 embryos of 2 cells was obtained. After 72 hours, there were 103 blastocyst cultures in both groups. Blastocyst development rate (BDR) culture with to endometriosis peritoneum fluid exposure was lower compared with the normal peritoneum fluid exposure (32.2% vs 56.9%, RR 0.5, 95% CI: 0.42-0.77). Hatching rate (HRI of blastocyst culture with endometriosis peritoneum fluid was lower than that of normal peritoneum fluid (35.1 % vs 92.4%, RR 0.38, 95%CI: 0.24-0.59). The exposure to 10% endometriosis peritoneum fluid lowered BDR significantly compared with the normal peritoneum fluid, 7.7% vs 43.9% (RR 0.18,95% CI: 0.07-0.42); while the exposure to 2.5 % BDR was significantly different, (64% vs 74%, RR 0.87,95% CI: 0.66-1.13). Exposure to 10% compared to 2.5% normal endometriosis peritoneum fluid showed significantly decreased BDR (p 0.05). There was a significant interaction between type of peritoneum fluid (endometriosis vs normal) and the concentration of peritoneum fluid (10% vs 2.5%1 in decreasing BDR (RR 0.17,95% CI: 0.04-0.65). Conclusion: Exposure to endometriosis peritoneum fluid lowered the blastocyst development rate and hatching rate as compared with that to normal peritoneum fluid on the development of the in- vitro mouse embryo model. Key words: embryonic development - peritoneum fluid - endometriosis
Nonmosaic 45, XO karyotype in a woman with Turner syndrome without any cognitive, psychosocial or behavioral deficiencies (A Case report) Enny S Pamuji Djaswadi Dasuki
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

A 22 year old woman with primary amenorrhea came to The Reproductive Endocrinology and Infertility Centre, University of Gadjah Mada, Dr.Sardjito Hospital. She had no abnormalities in cognitive, psychosocial or behavioral capacities, she had almost completed her university degree. On examination she had no secondary sexual development, no pathognomonic abnormal phenotype related to abnormal karyotype. There was no indication of family history associated ,;pecifically to the abnormal karyotype, phenotype feature of Turner syndrome and a specificThe patient karyotype analysis of blood sampling indicated 45XO, and it failed to demonstrate any mosaicism. Laparoscopic examination revealed a hypoplastic-lobulated-unicorns uterus, as streak gonad at the left side and no ovary at the other side.Keywords: Turner syndrome, non behavioral deficiencies. nonmosaic 45, XO karyotype, normal hormone level
Relationship between perimenopause symptoms and bone mineral density . Aida; Djaswadi Dasuki; Siswishanto Rukmono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 01 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.833 KB) | DOI: 10.19106/JMedScie004601201404

Abstract

The menopausal transition or perimenopause is associated with numerous symptoms. Womenentering perimonopause normally have menstrual irregular periods due to an imbalance of serumestradiol. Since the status of estrogen is known as an important determinant of bone massdensity, menopausal symptoms can be an effective indicator of bone status in women. Thisstudy aimed to evaluate the relationship between perimenopause symptoms and bone mineraldensity (BMD). This was an observational study using cross-sectional design conducted duringthree months periode from June to August 2010 in Kalangan Village, Banguntapan, BantulDistrict. Subjects were women between the aged 46-55 years who fulfilled the inclusion andexclusion criteria. After underwent history taking, subjects underwent physical examinationaccording to International Physical Activity Questionnaires (IPAQ). The BMD of subjects wasthan measured using dual-energy X-ray absorptiometry (DEXA). The relationship betweenperimenopause symptoms and BMD was then evaluated using multivariate analysis. The resultsshowed that perimenopause symptoms increase abnormal BMD of subjects 1.38 times higherthan those without perimenopause symptoms (RR=1.38; 95%CI 1.39-13.78; p=0.01). Inconclusion, the perimenopause symptoms is associated with the high proportion of abnormalBMD in women.
The influence of iron supplementation in pregnant women to the occurrence of low birth weight (LBW) babies in Palu, Central Sulawesi Sri Restu; Djaswadi Dasuki; R. Detty Siti Nurdiati Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 01 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.054 KB) | DOI: 10.19106/JMedScie004601201406

Abstract

Low birth weight (LBW) babies remains a major problem world wide as it causes a high rate ofneonatal morbidity and mortality. One effort to reduce the prevalence of LBW babies is byproviding iron supplementation to the pregnant women. The aim of this study was to examinethe relationship between iron supplementation program and prevalence of LBW babies in Palu,Central Sulawesi. This was an observational study with case control design. Subjects were allLBW babies from mothers that had accepted iron supplementation during pregnancy. Samplesize in this study was 87 for case and 87 for control. Bivariate analysis showed that ironsupplementation that not comply the program had a significant correlation with prevalence ofLBW babies (p=0.01). Other significant factors include abnormal hemoglobin level (p=0.01),body mass index (BMI) (p=0.02), educational level (p=0.02), multiparity (p=0.03), andgestational age (prematurity) (p=0.03). Multivariate analysis revealed that the strongest riskfactor for the occurrence of LBW babies was iron supplementation that not comply the program(OR= 3.82; 95% CI: 1.77- 8.22). Other risk factors were hemoglobin level (OR= 3.45; 95% CI:1.59-7.49), BMI (OR= 2.27; 95% CI: 1.05-4.91), gestational age (OR= 3.11; 95% CI: 1.45-6.67), multiparity (OR= 2.98, 95% CI: 1.36-6.51), and educational level (OR= 2.38, 95% CI:1.12-5.03). Based on the analysis, the strongest risk factors that affected the prevalence of LBWwas iron supplementation, abnormal hemoglobin level, gestational age, multiparity and educationlevel. In conclusion, iron supplementation during pregnancy that not comply with the programwas the strongest risk factor of LBW babies. The prevalence of LBW babies can be reduced bycontrolling of iron supplementation, hemoglobin level, BMI, gestational age, parity and education.
Zinc deficiency and school-age children’s memories . Setianingsih; Djaswadi Dasuki; Indria Laksmi Gamayanti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.861 KB) | DOI: 10.19106/JMedScie004603201403

Abstract

Zinc deficiency is associated with cognitive and motor delay in children. Moreover, it is associatedwith deficits in activity and attention in nutritional deficiency children. This study was conductedto evaluate the correlation between zinc deficiency with memory of children. A cross sectionalstudy design was employed from June 1st to 30th, 2013 among school-age children in KlatenDistrict, Central Java who met inclusion and exclusion criteria. Sixty five eligible children wereassessed their health, socio-economic and nutritional status. Hemoglobin levels were measuredby the standart cyanoblue method. Plasma zinc levels were analyzed with flame atomic absorptionspectrophotometry (AAS). Short-term memory (STM) was measured using the instrument WechslerIntellegence Scale for Children (WISC0 subtest Digit Span Memory Test and long term memory(LTM) was assessed using the recall of narrative. Independent t-test was used to compare theSTM or LTM between groups of each independent factors. Linear regression analysis was usedto determine the independent factors associated with the STM or LTM. The STM scores of thechildren with zinc deficiency (6.1 ± 1.3) was significantly higher than those with normal zinclevel (10.7 ± 3.1) [p=0.0004; 95%CI= -6.98 – (-2.14)]. However, the LTM for both group ofthe children were not significantly different (p=0.658; 95%CI= -3.16 – 2.01). A significantcorrelations between zinc levels, hemoglobin level and socio-economic status with the STM scoreswere observed (p<0.05), whereas nutritional status was not (p>0.05). In contrast, no significantcorrelations between zinc levels, hemoglobin levels, socio-economic status, nutritional status andthe LTM scores was observed in the school age children (p>0.05). In conclusion, zinc deficiency isassociated with STM loss in the school age children, however it is not associated with LTM loss.Moreover, hemoglobin level and socio-economic status are found to be independent factors forSTM loss, however they are not independent factors for LTM loss.
The Correlation Of Age, BMI, FBS And Akt Protein Expression In Granulosa Cells Of Polycystic Ovarium Anovulatory Efriyan Imantika; Djaswadi Dasuki; Ita Fauziah Hanoum
JUKE Unila Vol 4, No 07 (2014)
Publisher : Fakultas Kedokteran Universitas Lampung

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Abstract

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PERBANDINGAN SKOR DISMENOREA PADA PASIEN ENDOMETRIOSIS YANG MENDAPATKAN TERAPI ABLASI LAPAROSKOPI DILANJUTKAN GnRH AGONIST VERSUS ABLASI LAPAROSKOPI SAJA DI RSUP DR. SARDJITO YOGYAKARTA Marta Isyana; Djaswadi Dasuki; Diah Rumekti
JURNAL KESEHATAN REPRODUKSI Vol 1, No 3 (2014)
Publisher : Fakultas Kedokteran, Kesehatan Masyarakat dan Keperawatan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (496.786 KB) | DOI: 10.22146/jkr.5752

Abstract

PERBANDINGAN SKOR DISMENOREA PADA PASIEN ENDOMETRIOSIS YANG MENDAPATKAN TERAPI ABLASI LAPAROSKOPI DILANJUTKAN GnRH AGONIST VERSUS ABLASI LAPAROSKOPI SAJA DI RSUP DR. SARDJITO YOGYAKARTAMarta Isyana 1 , Djaswadi Dasuki2, Diah Rumekti3ABSTRACTBackground: Dysmenorrhea in endometriosis is a condition that adversely impacts the quality of life of women. The current treatment for dysmenorrhea in endometriosis is essentially palliative, since most of these treatment can only suppress disease progression and relieve its symptoms.Objective: To compare the difference in dysmenorrhea scores pre and post treatment of dysmenorrhea in endometriosis patients who received laparoscopic ablation followed with GnRH agonist versus laparoscopic ablation therapy alone.Method: This is an observational study with a retrospective cohort design. Endometriosis patients were identified through medical records at Dr Sardjito Hospital, Yogyakarta. Patients were categorized into laparoscopic ablation therapy followed by GnRH agonist group and laparoscopic ablation therapy only group. Evaluation of dysmenorrhoea scores were performed in 6 months after therapy.Result and Discussion: A total of 88 subjects (44 subjects in each group) were eligible and gave their consent to participate. Patients who received laparoscopic ablation therapy followed by GnRH agonist showed greater VAS difference pre and post treatment (6,27±0,22 vs 4,20±1,17,p<0,001) compared with only ablation laparoscopic. This difference was not affected by age, BMI, and endometriosis stage. Eleven of the 44 subjects who received laparoscopic ablation followed by GnRH agonists developed side effects. There were 7 people with hot flushes, 3 people with decreased bone mineral density and 1 people with dry skin, whereas no subject in laparoscopic ablation group alone experienced them.Conclusions: Laparoscopic ablation followed by GnRH agonist therapy was associated with greather difference in dysmenorrhea score pre and post treatment compared with laparoscopic ablation only. Laparoscopic ablation therapy followed with a GnRH agonist was associated with higher side effects.Keywords: endometriosis, laparoscopic ablation, GnRH agonist, visual analog scaleABSTRAKLatar Belakang: Dismenorea pada endometriosis adalah suatu kondisi yang memberikan dampak bermakna pada mutu kehidupan wanita. Penanganan dismenorea pada endometriosis saat ini pada hakikatnya masih belum berhasil menyembuhkannya, karena sebagian besar baru mampu menekan perkembangan penyakit dan menghilangkan gejalanya.Tujuan: Membandingkan selisih skor dismenorea sebelum dan setelah terapi pada pasien endometriosis yang mendapatkan terapi ablasi laparoskopi dilanjutkan GnRH agonist versus ablasi laparoskopi saja.Metode: Penelitian ini adalah penelitian observasional menggunakan rancangan penelitian kohort retrospektif. Pasien endometriosis diidentifikasi melalui rekam medis di RSUP DR Sardjito, Yogyakarta. Pasien dikelompokkan menjadi kelompok terapi ablasi laparoskopi dilanjutkan dengan GnRH agonist dan terapi ablasi laparoskopi saja.Evaluasi untuk skor dismenorea dilakukan pada jangka waktu 6 bulan setelah terapi. Hasil dan Pembahasan: Sebanyak 88 subyek (44 subyek dalam setiap kelompok) memenuhi kriteria penelitian dan memberikan persetujuan untuk diikutsertakan dalam penelitian. Pasien yang mendapatkan terapi ablasi laparoskopi dilanjutkan GnRH agonist menunjukkan selisih VAS sebelum dan setelah terapi yang secara signifikan lebih baik (6,22±0,22 vs 4,20±1,17;p<0,001) dibandingkan dengan ablasi laparoskopi saja. Perbedaan ini tidak dipengaruhi oleh umur, BMI, maupun derajat endometriosis. Sebelas dari 44 subyek yang mendapatkan ablasi laparoskopi dilanjutkan GnRH agonist mengalami efek samping, yaitu 7 orang mengalami hot flushes, 3 orang mengalami penurunan densitas masa tulang dan 1 orang mengalami kulit kering, sedangkan tidak ada subyek dalam kelompok ablasi laparoskopi saja yang mengalami efek samping.Kesimpulan: Terapi ablasi laparoskopi yang dilanjutkan dengan GnRH agonist berhubungan dengan selisih skor dismenorea sebelum dan setelah terapi yang lebih tinggi dibandingkan dengan terapi ablasi laparoskopi saja. Terapi ablasi laparoskopi yang dilanjutkan dengan GnRH agonist berhubungan dengan tingkat efek samping yang lebih tinggi.Kata kunci: endometriosis, ablasi laparoskopi, GnRH agonist, visual analog scale 1,2,3 Bagian Obstetri dan Ginekologi Fakultas Kedokteran Universitas Gadjah Mada
HUBUNGAN ANTARA STRESOR PSIKOSOSIAL DENGAN GANGGUAN MENSTRUASI PADA REMAJA SMP PASCA ERUPSI MERAPI DI KECAMATAN CANGKRINGAN KABUPATEN SLEMAN YOGYAKARTA Anik Dwi Marga; Sumarni Dw; Djaswadi Dasuki
JURNAL KESEHATAN REPRODUKSI Vol 2, No 3 (2015)
Publisher : Fakultas Kedokteran, Kesehatan Masyarakat dan Keperawatan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkr.12655

Abstract

HUBUNGAN ANTARA STRESOR PSIKOSOSIAL DENGAN GANGGUAN MENSTRUASI PADA REMAJA SMP PASCA ERUPSI MERAPI DI KECAMATAN CANGKRINGAN KABUPATEN SLEMAN YOGYAKARTAAnik Dwi Marga 1, Sumarni DW 2, Djaswadi Dasuki 3ABSTRACTBackground: Merapi eruption can cause tremendous damage, not only physically but also mentallywhich will trigger the emergence of mental disorders in adolescence and even into adulthood. After theeruption of Merapi, adolescents and their families should move from shelters to permanent residence.Resettlement to permanent residence had resulted in a new variety of stressors. The new stressors wouldaffect the release of LH (luteinizing hormone) and FSH (follicle-stimulating hormone) which may affectthe occurrence of menstrual disorders. Menstrual disorder might interfere with learning achievement inadolescence.Objective: To determine the relationship between psychosocial stressors with menstrual disorders infemale junior high school students after the eruption of Merapi in Cangkringan Sleman YogyakartaMethod: This study is a observational with cross-sectional design and combined with a qualitativeapproach. Respondents in this research is class VII students who were menstruating in CangkringanSleman. Samplings method used was total sampling. The research instrument was a questionnairepsychosocial stressors (IPSP), menstrual disorder questionnaire (SPAF) and the behavioral aspects of thequestionnaire. Datas were analysed using Chi-Square test by looking at the value of prevalence ratio andlogistic regression at the significance level of p <0.05 with a confidence interval (CI) of 95%.Result and Discussion: There was a significant correlation between psychosocial stressors and menstrualdisorders (OR; 6.47; CI = 1.436 to 29.165)Conclusion: Adolescents who experienced severe psychosocial stressors six times more likely to experiencemenstrual disorders compared to adolescents who experience mild psychosocial stressorsKeywords: Psychosocial Stressors, menstrual disorders, post-eruption of Merapi, Youth,SMP.ABSTRAKLatar Belakang: Erupsi Merapi dapat menyebabkan kerusakan yang luar biasa, tidak hanya fisik tetapijuga mental yang akan memicu munculnya gangguan jiwa pada remaja bahkan sampai masa dewasa.Pasca erupsi Merapi, remaja dan keluarganya harus pindah dari huntara ke huntap. Perpindahan tempattinggal ke huntap inilah yang mengakibatkan berbagai stresor-stresor baru.. Stresor baru tersebut akanmempengaruhi pelepasan LH (Luteinizing Hormone) dan FSH (follicle-stimulating hormone) yang dapatmempengaruhi terjadinya gangguan menstruasi. Masalah menstruasi jika tidak mendapatkan penangananyang benar dapat mengganggu prestasi belajar remaja.Tujuan: Untuk mengetahui hubungan antara stresor psikososial dengan gangguan menstruasi pada remajaSMP pasca erupsi Merapi di Kecamatan Cangkringan Kabupaten Sleman YogyakartaMetode: Penelitian ini merupakan jenis penelitian non eksperimen dengan metode observasional denganrancangan cross-sectional yang dilengkapi dengan pendekatan kualitatif. Responden dalam penelitianini adalah Siswi kelas VII SMP yang sudah menstruasi di Kecamatan Cangkringan Kabupaten Sleman.Pengambilan sampel penelitian dengan total sampling. Instrumen penelitiannya adalah kuesioner stresorpsikososial (IPSP), kuesioner gangguan menstruasi (SPAF) dan kuesioner aspek perilaku. Analisis datamenggunakan Uji Chi-Square dengan melihat nilai RP (Rasio Prevalensi) dan regresi logistik pada tingkatkemaknaan p<0,05 dengan Confidence interval 95%.Hasil dan Pembahasan: Remaja yang mengalami stresor psikososial berat beresiko 6,47 kali lebih besaruntuk terjadi gangguan menstruasi (CI 95% 1,436-29,165)Kesimpulan: Remaja yang mengalami stresor psikososial berat mempunyai resiko 6 kali lebih besarmengalami gangguan menstruasi dibandingkan dengan remaja yang mengalami stresor psikososial ringanKata Kunci: Stresor psikososial, Gangguan menstruasi, Pasca Erupsi Merapi, Remaja, SMP.1 Anik Dwi Marga dari Magister Ilmu Kesehatan Masyarakat, Fakultas Kedokteran, UGM2 Sumarni DW dari Bagian Kedokteran Jiwa Fakultas Kedokteran, UGM3 Djaswadi Dasuki Bagian Obstetri Ginekologi, Fakultas Kedokteran, UGM
Pengetahuan tentang Infeksi Menular Seksual dengan Perilaku Seksual Tidak Aman pada Remaja Putri Maluku Tenggara Barat di Daerah Istimewa Yogyakarta Firmina Th Kora; Djaswadi Dasuki; Djauhar Ismail
JURNAL KESEHATAN REPRODUKSI Vol 3, No 1 (2016)
Publisher : Fakultas Kedokteran, Kesehatan Masyarakat dan Keperawatan UGM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jkr.13880

Abstract

Background: Based on the Indonesian Demographic and Health Survey (IDHS, 2012), adolescents’ knowledge (females aged 15-24 years) about STI (Sexually Transmitted Disease) is still low under 50%. A total of 16.46% Indonesian adolescents have had sex, 46.26% of them have been doing it actively, first sex occurred in below 20 years of age.Objective: To determine the relationship between knowledge about sexually transmitted infections with unsafe sexual behavior.Method: This study is a quantitative and qualitative with cross-sectional study design. Subjects in this study were all female adolescents of West Southeast Moluccas who are continuing education in Yogyakarta. Univariable and bivariable data analysis was conducted using chi-square test statistic. Multivariable analysis was conducted using logistic regression to estimate the effective model.Result and Discussion: Low knowledge STI is practically and statistically related to unsafe sexual behavior with a p-value <0.05, RP 1.71 and CI 1.07 to 2.70. Low knowledge about STIs in adolescents increase the risk by 1.7 times for unsafe sexual behavior. Conclusion: 67,1% female adolescent who have unsafe sexual behavior. Female adolescents who have knowledge about STIs low 65.7%. Low knowledge STI significantly increases unsafe sexual behavior in adolescent girls. Unsafe sexual behavior conducted by adolescent female are sexual intercourse before the age of 20 not using condoms during sexual intercourse, and multiple sexual partner.Keywords: Knowledge of Sexually Transmitted Infections, Unsafe Sexual Behavior, Female Adolescent
Co-Authors . Aida Abdul Wahab Abrori, Abrori Abrori, Abrori Ade Ana Adelina Amelia Adi Adi Adinda Putri Sari Dewi Agus Sudiana Nurmansyah Agustin Endriyani, Agustin Ahmad Hamim Sadewa Ahsanudin Attamimi Almumtahanah, Almumtahanah Anak Agung Istri Sri Wiadnyani Andriana Kumala Dewi Anifah, Fulatul Anik Dwi Marga Annisa R Annisa Rahmawati ANNISA RAHMAWATI Annisa Rahmawati Annisa Rahmawati Arfan, Iskandar Ariani, Desti Dwi Arief Boediono Asep Novianto Asmar Yetty Zein Asmar Yetty Zein, Asmar Yetty Aswita, Aswita Atik Triratnawati Aulia, Ayuning Ayu Diana Meilantika Ayu Sugiarti Banun Kusumawardani Batubara, Irwan Bhakti, Wida Kuswida Blessilia, Eligia Cahya Skania, Pratiwi Cau Kim Jiu Cau Kim Jiu Dameria, Netty Katrina Daniel Happy Putra Dessy Hertati Detty Siti Nurdiati Dewi, Adinda Putri Sari Dewi, Fovilia Dewi, Ika Laksita Dewi, Suryani Puspa Dhesi Ari Astuti, Dhesi Ari Diah Fauri Yani Diah Rumekti Diah Rumekti Diah Rumekti Hadiati Dita Maria Virginia Djauhar Ismail Djauhar Ismail Dwi Hidayah Dwi Hidayah Edy Meiyanto Efriyan Imantika Elfrida Ratnawati Elisa Elisa Endang Purwaningsih Erlando, Zeri Erwan Syahrudin Ery purwanti Eva Mayandari Firmina Th Kora Hanafi Hanafi Handayani1, Leni Wahyu Hapis, Hapis Hapiz Haryanto Haryanto Herlin Fitriana Kurniawati Heru Pradjatmo Hidayah hidayah Hidayah, Hidayah Husnawati Husnawati Imran Imran Indah Budiastutik Indah Indah Indri Erhwani Indri Erwhani Indria Laksmi Gamayanti Indria Laksmi Gamayanti Irham Irham Irham Irham Irwan Taufiqurahman Isnaini Putri Ita Fauzia Hanoum, Ita Fauzia Ita Fauziah Hanoum K, Herlin Fitriana Kadarsih Soejono, Sri Kadarsih Soejono, Sri Kadek Yuke Widyantari Kardiatun, Tutur Kartini, Farida Khairillah, Yuyun Nisaul Khoir, Fathul Kora, Firmina Th Lamana, Aspia Legawati Legawati, Legawati Leni Wahyu Handayani1 Lestari Makmuriana Lestari Makmuriana Lestari, Lilis Lilik Ariyanti Lily Marliany Surjadi Lukita, Yenni M Taufik Madarina Julia Mardiana Mardiana Mardiani Mardiani Mardiyani, Ridha Marga, Anik Dwi Marlenywati Marlenywati Marsetyawan Soesatyo Marsetyawan Soesatyo Marta Isyana Marta Isyana Masitha, Wulan Mauana, Andi Mayarestya, Nita Putriasti Meisy Meisy Menik Sri Daryanti Menik Sri Daryanti, Menik Sri Meta Trisyya Mubasysyir Hasanbasri MULYOTO PANGESTU Mumtahanah, Al Mustofa Mustofa Nora Gracesara Nuniek Setyo Wardani Nur Ahlam Nadia Putri Nurhaya S Patui Nurmansyah, Agus Sudiana Nurmeini Nuruniyah Nuruniyah, Nuruniyah Nuzula, Rizka Firdausi Ova Emilia Parliani, Parliani Pinandari, Anggriyani W. Pinda Hutajulu Pinda Hutajulu, Pinda Purwanto, Dedy Putri, Nita Tri Putri, Nur Ahlam Nadia R. Sapto Hendri Boedi Soesatyo Rahayu, Kusnia Wati Rahayu, Meyi Setiawati Ramanda, Galih Dwiki Retno Mawarti, Retno Ridha Mardiani Ridha Mardiani Ridha Mardiani Ridha Mardiani riwayati riwayati Rizky, Ayu Rona Eka Kusuma Rosdarni Rosdarni Rosdarni Rosdarni, Rosdarni Rukmono Siswishanto Samad, Abdu Sari, Nurmila Selly K Setianingsih Setianingsih Setianingsih, S Shofwal Widad Siswanto Agus Wilopo Siti Masdah Sri Ariyanti Sri Ariyanti Sri Handayani Sri Haryati Sri Kadarsih S Sri Kadarsih S, Sri Sri Restu Sugiarti, Ayu Sulchan Sofoewan, Sulchan Sulistyaningsih, Sulistyaningsih - SUMARNI Sumarni Djoko Waluyo Sumarni Djoko Waluyo, Sumarni Djoko Sumarni DW Sumarni DW, Sumarni SUMIYATI SUMIYATI Suriadi Jais Susilawati, Budi Susilawati, Miya Sutrisno Sutrisno Sutrisno, Sutrisno Topan Iman Sanjaya Totok Utoro Totok Utoro Tri Hastuti Tri Hastuti tri wahyuni Tri Wahyuni Tri Wahyuni Tri Wahyuni Tunjung Wibowo Tunjung Wibowo Tuti Nuraini Tuti Nuraini Ujiana, Sugi Ultari, Cici Uswatul Hasanah Utami, Fitria Siswi Virarisca, Sheilla Vivit Wiyandani Wahyuhidaya, Pratika Wahyuni, Budi Wibowo, Yudi Herdianto Widya Asmara Wuriani Wuriani Yani, Diah Fauri Yayi Suryo Prabandari Yuanita, Irma Linda Yundari, Yundari Zerry Erlando