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Selection of Isolates of Entomopathogenic Fungi and the Bioefficacy of Their Liquid Production against Leptocorisa oratorius Nymphs SITI HERLINDA; SRI INDAH MULYATI; . SUWANDI
Microbiology Indonesia Vol. 2 No. 3 (2008): December 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.708 KB) | DOI: 10.5454/mi.2.3.9

Abstract

Entomopathogenic fungi are fungi pathogenic to insects and are widely used as biocontrol agents for insect pests. The aim of this research was to study the virulence of Beauveria bassiana and Metarhizium sp. isolates and to evaluate the efficacy of liquid production of those fungi against Leptocorisa oratorius (rice bug). Twelve isolates of B. bassiana and five isolates of Metarhizium sp. were used in this research. Selection result of B. bassiana isolates on third-instar rice bug nymphs showed that the isolate KBC caused the highest mortality rate (93%), while the lowest (46%) was caused by the isolate BBY 725. The shortest time needed to produce 50% mortality (Lethal time, LT50) was 3.52 days (isolate KBC). The longest time (10.36 days) was produced by isolate SLSS. The mortality of rice bug nymphs caused by Metarhizium isolates was only 50-62%. The shortest LT50 of Metarhizium (5.75 days) was produced by isolate Mtm, while the longest (7.46 days) was produced by isolate Mpx. Bioefficacy tests on six kinds of liquid formations of entomopathogenic fungi indicated that all were effective, mostly with LT50 d” two days. The mortality rates of rice bug nymphs caused by bioefficacy of fungus liquid production was generally above 85% up to 100%. The liquid media for entomopathogenic fungi performed better compared with solid media (SDA), as indicated by the greater mortality rate and shorter LT50.
Identification and Selection of Entomopathogenic Fungi as Biocontrol Agents for Aphis gossypii from South Sumatra SITI HERLINDA; CHANDRA IRSAN; REKA MAYASARI; SELLY SEPTARIANI
Microbiology Indonesia Vol. 4 No. 3 (2010): December 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.401 KB) | DOI: 10.5454/mi.4.3.%p

Abstract

Aphid, Aphis gossypii is a vector of curly virus disease. The damage of chili due to its feeding  is only 35% and it can achieved 100% if the damage caused by the aphid as a vector.  The objectives of this research were to explore, to isolate, to identify, and to select entomopathogenic fungi as biocontrol agents for A. gossypii. The fungi were explored using insect bait in soil and collected infected insects from South Sumatra, Indonesia.  Then, the fungi were isolated and identified, and finally the bioefficacy tests were done using 1 x 106 conidia mL-1 against the third instar of A. gossypii. The explorations found 25 isolates of enthomopathogenic fungi consisting 10 isolates of Beauveria bassiana and 15 isolates of Metarhizium anisopliae. Selection of the fungi isolates on the aphid nymphs showed that isolate BPM isolated from Pseudoplusia chalcites caused the highest mortality rate (80.80%), while the lowest (47.20%) was caused by the isolate BAgTb isolated from A. gossypii.  The shortest time needed to produce 50% mortality (Lethal Time 50) was 2.54 days (isolate of Chrysodeixis chalcites from Muarasiban). The longest time (3.66 days) was produced by isolate of Tenebrio molitor from Tanjung Raja.
KELIMPAHAN DAN KEKAYAAN ARTROPODA PREDATOR PADA TANAMAN PADI YANG DIAPLIKASI BIOINSEKTISIDA BACILLUS THURINGIENSIS Chandra Irsan; Fina Sunariah; Siti Herlinda; Yuanita Windusari
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 16 No. 1 (2016): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.276 KB) | DOI: 10.23960/j.hptt.11642-50

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Abundance and species richness of the predatory arthropods on paddy treated with Bacillus thuringiensis bioinsecticide. Application of bioinsecticide from bacterial entomopathogen has not been reported yet that can decrease abundance and species richness of predatory arthropods, such as spiders and predatory insects. This research was aimed to analyze the abundance and species richness of predatory arthropods paddy fields applied by B. thuringiensis bioinsecticide on paddy in fresh swamp area. Areas observed were 2 ha paddy field’s at Situ Bagendit variety. Predatory arthropods inhabiting canopy were sampled using sweep but soil dwelling arthropods were trapped using pitfall traps. The result showed that abundance of spiders inhabiting canopy decreased significantly after B. thuringiensis bioinsecticide application on paddy compared to control without bioinsecticide but the abundance of predatory insects were not significantly affected by the bioinsecticide. Application of the bioinsecticide did not significantly affect the abundance and species richness of soil dwelling predatory arthropods, such as spiders and predatory insects.
POPULASI DAN SERANGAN KUTU PUTIH PEPAYA, PARACOCCUS MARGINATUS (HEMIPTERA: PSEUDOCOCCIDAE) PADA TANAMAN PEPAYA DI DAERAH DATARAN RENDAH SUMATERA SELATAN Rosdah Thalib .; Rossi Fachrullah Rozi; Triani Adam .; Khodijah . .; Siti Herlinda .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (872.28 KB) | DOI: 10.23960/j.hptt.214136-141

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Populations and damage by the papaya mealybug Paracoccus marginatus (Hemiptera: Pseudococcidae) on papaya inlowland areas of South Sumatera. One of the main problems in papaya cultivation today is the presence of a newlyintroduced pest, the white papaya mealybug, Paracoccus marginatus (Hemiptera: Pseudococcidae). This study aimed toobserve the mealybug populations and its damage percentage in papaya plants and to identify its host plants in SouthSumatera. The study was carried out on the fruit and vegetable producing area in Indralaya, South Sumatera. The survey wasconducted on papaya plantation plots with a minimum size of 1000 m2. Data from the observation indicated that populationsof P. marginatus on papaya fruit were not significantly different to those on papaya leaves, except for the first observation inthe rainy season. In general, the percentages of mealybug damages were not affected by the season and the percentages ofdamage on the leaves were not different from those on the fruits of the papaya plants. Inventory of P. marginatus host plantson the area resulted in a total of 12 species of host plants dominated by dicotyledonous plant species. The host plants werefrom 11 families, i.e. Anacardiaceae, Caricaceae, Cucurbitaceae, Euphorbiaceae, Fabaceae, Myrtaceae, Rosaceae, Rutaceae,Sapindaceae, Sapotaceae, and Solanaceae.
Patogenisitas Isolat Beauveria bassiana dan Metarhizium anisopliae asal Tanah Lebak dan Pasang Surut Sumatera Selatan untuk Agens Hayati Scirpophaga incertulas Rosdah Thalib; Redi Fernando; . Khodijah; Dewi Meidalima; Siti Herlinda
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.207 KB) | DOI: 10.23960/j.hptt.11310-18

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Pathogeicity of Beauveria bassiana and Metarhizium anisopliae Isolates from Fresh Swamp and Tidal Lowland, South Sumatra for Scirpophaga incertulas Biological Agents. The objectives of the research weret o explore and to determine the pathogenicity of entomopathogenic fungi againts the larvae of Scirpophaga incertulas, and to measure conidial viability and density of the fungi. The method for fungi exploration used larvae of Tenebrio molitor baiting submerged in the soil. The soil was taken from fresh swampand tidal lowland rice in South Sumatra. From the exploration study, we found two species of entomopathogenic fungi: Beauveria bassiana and Metarhizium anisopliae. Mortality of S. incertulas larvae that had been treated topically with fungal conidia (1x106 conidia ml-1) varied among the isolates. The highest mortality (98.33%) caused by BPlus isolate of B. Bassiana and the lowest by MtmIn isolate of M. anisopliae (57.50%) and BTmTr isolate of B. bassiana (57.50%). The fungal colonies grew fast from the second day up to the fourth day after incubation but the growth became slow after the fifth day. The highest conidial density was resulted by BPcMs of B. bassiana isolate (63.33x106 conidia ml-1) but this density was not significantly different from that of the BPlus of B. bassiana isolate (63.11x106 conidia ml-1). The lowest conidial density found in BTmTr of B. bassiana isolate (20.97x106 conidia ml-1) . The isolate B. bassiana was more effective than M. anisopliae againt the larvae of S.incertulas.
Uji Efikasi Bioinsektisida Jamur Entomopatogen Berformulasi Cair terhadap Plutella xylostella (L.) Di Laboratorium Haperidah Nunilahwati; Siti Herlinda; Chandra Irsan; Yulia Pujiastuti; . Khodijah; Dewi Meidelima
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.748 KB) | DOI: 10.23960/j.hptt.11352-60

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Efficacy test of liquid bio-insecticide of entomopathogenic fungi in control against Plutella xylostella in the laboratory. The insect pest P. xylostella could reduce crop production of Brassicaceae. The aim of research was to test the efficacy liquid bio insecticide with active ingredient of Beauveria bassiana and Metarhizium anisopliae fungi to control P. xylostella. Bio-insecticide was applied by spraying on mustard leaves infested with 50 individuals of third instar larvae of P. xylostella and a density of 1x106 conidia ml-1. Larval mortality was observed every 2 hours and LT50 of larvae was calculated. The study showed that the highest percentage of mortality found in Mt ES and Mt ES (cf) isolates was 99.6%, the lowest mortality at Mt NES isolate was 96.80%. LT50 and LT95 values Bb ES were the lowest i.e. 2.04 days and 2.95 days. The highest LT50 and LT95 of Mt NES isolate were 2.24 days and 3.32 days. The liquid bio-insecticide of entomopathogenic fungus B. bassiana and M. anisopliae were effective to control the larvae of P. xylostella.
Keanekaragaman, Dominasi, Persebaran Spesies Penggerek Batang Padi dan Serangannya pada Berbagai Tipologi Lahan di Provinsi Jambi . Wilyus; Fuad Nurdiansyah; Asni Johari; Siti Herlinda; Chandra Irsan; Yulia Pujiastuti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 13 No. 1 (2013): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (491.931 KB) | DOI: 10.23960/j.hptt.11387-95

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Diversity, domination, and distribution of rice stem borer species and its damage in various land typologies in Jambi Province. The research was conducted to analyze the diversity, domination, species distribution of rice stem borers (RSB) and its damage on various land typologies in Jambi Province. The research was carried out using survey method, from December 2010 until June 2011. Samples of RSB were collected from tidal swamp in Tanjung Jabung Timur District, swampy area in Muaro Jambi District, rainfed lowland in Sarolangun District, irrigated lowland in Merangin District, and irrigated upland in Kerinci District and Sungai Penuh District. The results showed that there were five spesies of RSB found in Jambi Province. Scirpophaga incertulas Walker (yellow stem borer) was the most dominant of RSB, followed by Sesamia inferens Walker (pink stem borer), Chilo suppressalis Walker (striped stem borer), Chilo polychrysus Meyrick (dark-headed stem borer), and Scirpophaga innotata Walker (white stem borer). The distribution of S. incertulas, S. inferens, C. suppressalis and C. polychrysus were in all of rice field in Jambi Province, but that of S. innotata was limited over tidal swamp and rainfield lowland area. The RSB damage rate was lower than economic thereshold.
SPESIES PARASITOID LIPAPHIS ERYSIMI ASAL SUMATERA SELATAN DAN VARIASI PARASITISASINYA PADA TUMBUHAN INANG YANG BERBEDA Siti Herlinda; Erise Anggraini; Chandra Irsan; Abu Umayah; Rosdah Thalib; Triani Adam
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 2 (2012): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (136.038 KB) | DOI: 10.23960/j.hptt.212111-118

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Lipaphis erysimi (Hemiptera: Aphididae) is one of the main insect pests on brassicaceous crops. Aphids attack crops by sucking sap from its hosts. Densed population in the initial phase of crop growth could inhibit growing, loss in yield up to 100%. Experiments were aimed to explore and identify species of L. erysimi parasitoid in highland areas of South Sumatra, and to determine potency of the parasitoids to parasitize L. erysimi in laboratory. The highest parasitism level of L. erysimi (72.40%) was found on 200 aphids which reared on B. juncea. While, the second highest parasitism level (65.67%) was found on N. indicum. The number of mummies found on N. indicum was less than on B. juncea. Of all observations, every treatment on every tested plant showed that female D. rapae emerged more than male D. rapae (female bias). The two parasitoids species that parasitized L. erysimi were Diaeretiella rapae and Aphidius sp. Parasitisation at several location in South Sumatera was highly diverse. D. rapae was the most abundant parasitoid species on several high lands at South Sumatera.
EKSPLORASI, ISOLASI DAN SELEKSI JAMUR ENTOMOPATOGEN PLUTELLA XYLOSTELLA (LEPIDOPTERA: YPONOMEUTIDAE) PADA PERTANAMAN CAISIN (BRASSICA CHINENSIS) DI SUMATERA SELATAN Haperidah Nunilahwati; Siti Herlinda; Chandra Irsan; Yulia Pujiastuti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 1 (2012): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (692.329 KB) | DOI: 10.23960/j.hptt.1121-11

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Plutella xylostella is the most destructive insect pests of the brassicae family. The research objective was to explore, isolate and select entomopathogenic fungi as biological agents for control of P. xylostella. This study used 20 fungal isolates originating from soil and infected insects around the farmers’ field in lowland and highland of South Sumatra. The fungal isolates were tested to third instar larvae of P.xylostella. The suspension of entomopathogenic fungus was topical inoculated with a density of 1x106 conidia ml-1 on the test insect and five replicates. The result showed that the highest (83%) and the lowest (41%) mortality of the larvae P.xylostella was induced by fungal BPluS and BNIPTr, respectively. Moreover, the shortest (2.1 days) and the highest (4.3days) lethal times of the infected host were induced by fungal BPluS and BNIPTr, respectively.
POTENSI PARASITOID TELUR PENGGEREK BATANG PADI KUNING Scirpophaga incertulas Walker PADA BEBERAPA TIPOLOGI LAHAN DI PROVINSI JAMBI . Wilyus; Fuad Nurdiansyah; Siti Herlinda; Chandra Irsan; Yulia Pujiastuti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 1 (2012): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.547 KB) | DOI: 10.23960/j.hptt.11256-63

Abstract

The research was conducted to analyze the diversity, dispersal and domination of the egg parasitoid species of the yellow rice stem borer Scirpophaga incertulas Walker on several land typologies in Jambi Province. The study was conducted by survey method, from December 2010 until June 2011. Samples of egg parasitoids of S. incertulas were collected by baiting parasitoids with eggs of S. incertulas. The results of the research showed that there were three spesies of the egg parasitoids of S. incertulas found in Jambi Province, the most dominant was Telenomus rowani Gahan, followed by Trichogramma japonicum Ashmead and Tetrastichus schoenobii Ferriere. T. rowani and T. japonicum were found in all wetland ecosystems in Jambi Province (tidal swamp, swampy area, rainfed lowland, lowland technical irrigation, and upland technical irrigation), while T. schoenobii was found only in tidal swamp, swampy area, and lowland technical irrigation. The highest species diversity of egg parasitoid of S. incertulas was found on tidal swamp (Shannon index 1.047), followed by swampy area, lowland technical irrigation, rainfed and upland technical irrigation area. The average of proportion of egg masses parasitized by T. rowani, T. japonicum and T. Schoenobii were 22.58, 6.18 and 2.68% respectively. The average of individual eggs parasitized by T. rowani, T. schoenobii, and T. japnicum were 8.41, 1.67 and 1.47% respectively.
Co-Authors . Khodijah . Khodijah . Khodijah . Suwandi Abbellia, Dea Abu Umayah Abu Umayah Agie Nurwati AGUSMAN JAYA Ahmad Muslim Amarilisa Kusuma, Amarilisa Amelia Herwanti, Andriani Amin Hasan Andi Wijaya ANDI WIJAYA Andi Wijaya Anita Setyawati Anriani, Nurul Apriyani, Eka Apryanti, Hesti Ardellta, Nasya Aries Denta Putra Arini, Ririn Arrwais, Rhani Arsi Arsi Arsi Arsi Arum Setiawan Asdarina Asdarina Asmawati, Luluk Asni Johari Asti, Meirin Astry Wulandari Athalina, Ghita Aulia Chandra, Fachdilla Avinindy Inayda Devianti Az Zahra, Aisyah Dwi B. Gunawan Bambang Gunawan Bambang Gunawan Barokah Alburaidah, Muhammad Nur Benyamin Lakitan Carsilladepasha, Al Fath Chaeratunnisa, Elsa Chandra Irsan Chandra Irsan Chandra Irsan Chandra Irsan Chandra Irsan Chandra Irsan Chandra Irsan Chandra Irsan Chandra Irsan Chandra Irsan Chandra Irsan CHANDRA IRSAN Chandra Irsan Chandra Irsan Chandra Isran Cindi, Aulia Cinta, Lara Damanhuri, Damanhuri Daniel Happy Putra Dedeh Sumiati Dewi Meidalima Dewi Meidelima Dr. Susilawati Susilawati Dwitami, Egga Eka Yulistin Elfita Elfita Elfita Endang Susilowati Eneng Hikmah Dewi Puspita Erfansyah Erfansyah Erise Anggraini Erise Anggraini, Erise Erizal Sodikin Erna Siaga Fadlullah Fadlullah Fahmie Firmansyah Farid Ibnu Wahid Fila Sunariah Fina Sunariah Firdaus Sulaiman, Firdaus Firmansyah Firmansyah Fitra Gustiar, Fitra Fuad Nurdiansyah Ghani Prabawati Hadi, Buyung A. Hamzah Hasyim Haperidah Nunilahwati Harman Hamidson Harman Hamidson Harun, Muhammad Umar Hasbi Hasbi hasbi hasbi Hendi Firdaus Hendra Hendra Hendri Candro Nauli Manalu Hong Lau, Wei Humairoh, Balqis Ilaika Maulida Ilham Farid Ilmawan, Kalam Imam Nur Rahman Indayani Indayani, Indayani Irmawati Irmawati Irsan, Chandra Irsan Irwan Djumen Isti Rusdiyani Isti Rusdiyani Ita Ristika Sari Jamhari Jamhari Jati Purnama Achmad Jelly Milinia Puspita Sari Juliastuti, Juliastuti karenina, Tili Kartika Kartika Khodijah . . Khodijah Khodijah Khodijah Khodijah Khodijah Khodijah Khoirul Ikhsanudin Hanif Khoirul Ikhsanudin Hanif Komang Agus Darmawan Kurniawan, Andri Laila Kartini Lailaturrahmi Lailaturrahmi Laily Ilman Widuri, Laily Ilman Lilik Nur Kholidah Lina Budiarti LINA BUDIARTI Lindiana Lindiana Liska Berlian Lukmanul Hakim LULUK ASMAWATI M. Umar Harun Madya, Sakha Prawira Maharani, Shara Marieska Verawaty Marlina, Dewi Nini Maryati Maryati Maschamah, Rahmaningrum Meihana Meihana Meitha Sari, Dwi Merlisma Sari, Selvy Miftahul Jannah Milinia Puspita Sari, Jelly Mimma Gustianingtyas Muhammad Ammar Muhammad Darif Muhammad Umar Harun Muthia Amalia Cendrawati Nabiha Putra, Naka Rashad Nabila Adinda, Puspa Gemala Nabila, Ade Nandyta, Alifya Nesya Neni Yuliani Netaria, Netaria Ningrahayu, Seviyanti Ningrum, Rafika Cahya Noni Octariati Nugraha, Muhamad Ardian Nulhakim, Lukman Nur Fitriyani Nurhaeda Gailea Nurhakim, Lukman Nursalim, Yossi Aprian Oktaviani Oktaviani Otong Hidayat Padila Padila, Padila Panjaitan, Hendrik Haposan Prayudi, Rafi Prim Suardi Yudanto, Marcelinus Rido PURNAMA HIDAYAT Puspita Sari, Jelly Milinia Putri, Jeni Angela R Anwar Efendi R Putra Munandar Radix Suharjo Raffi, Muhammad Rafika Dewi Rahmawati, Aulya Ramayanti, Indri Ratna Sari RAUF, AUNU Redi Fernando REKA MAYASARI Reksa Adya Pribadi Rinda Fajrin Aldina, Rinda Fajrin Rindiani, Dellania Eka Riris Andriani Riska Resita Riski Anwar Efendi Risnu Munandar Riyanto Riyanto Riyanto Riyanto Rizkie, Lilian Roma Mantin Romli Ardie Rosdah Thalib Rosdah Thalib Rosdah Thalib Rosdah Thalib Rosdah Thalib . Rossi Fachrullah Rozi Roy Pardingotan Rury Rubykania S Imam Nugraha S. P. Estuningsih Salsabila, Sabrina Salsabilla Sirega, Septiana Rezeki Sari, Fitria Sari, Jelly Milinia Puspita Saripudin Saripudin Sekarweni, Hastin Wulan SELLY SEPTARIANI Sholih Sholih Siswandi Siswandi Siti Aminah Sitompul, Kristian B. Soemartono Sosromarsono Soleha Soleha SRI INDAH MULYATI Sudi Aryanto Suherman, Suherman Suherman, Suherman Sumikarsih, Eka Sumini Sumini, Sumini SUPARMAN . Suparman SHK suparman suparman Suparman Suparman Suparno, Suparno Suroso Mukti Leksono Susilawati . Susilawati Susilawati Susilawati, Susilawati Suwandi . Suwandi Suwandi Suwandi Suwandi Suwandi Suwandi Suwandi Suwandi Suwandi Suwandi Thosin, Thosin Tili Karenina Tri Astuti Triani Adam Triani Adam Triani Adam Triani Adam . Trianza Putri, Anya Triyanti, Yunita Ujang Jamaludin Utomo Kartosuwondo Vini Utami Wagiyanti Wagiyanti Waluyo Waluyo Weni, Hastin WS Wilyus Wilyus Wiwik Pratiwi Yuanita Windusari Yuanita Windusari Yudianti, Yudianti Yulia Pudjiastuti YULIA PUJIASTUTI Yulia Pujiastuti YULIA PUJIASTUTI Yulistin, Eka