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UTILIZATION OF RHIZOSPHERE FUNGI TO CONTROL Fusarium oxysporum f.sp. capsici IN VITRO I Made Sudarma; Ni Made Puspawati; Ni Wayan Suniti; I Nyoman Wijaya; I Gusti Ngurah Bagus
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Fusarium caused wilt disease in chilli pepper and destroyed some farmer crops. Results ofpreliminary research has been discovered that the disease caused by Fusarium oxysporum f.sp. capsici.The alternative environmental friendly method is to find antagonist microbes which is located in therhizosphere of healthy pepper plants. This study aims to find out potentially antagonistic fungi tocontrol Fusarium wilt disease on pepper plants. The fungi were isolated by soil dilution method orviable plate count method on Potato Dextrose Agar medium with antibiotic Livoplaxacin (25%, w/v).Rhizosphere fungi from healthy pepper plants had been identified. A total of 63 spesies belong to 4genera included Penicillium (45 species), Aspergillus (6 species), Trichoderma (9 species) andCandida (3 species). The highest percentage of distribution of rhizosphere fungi are P. digitatum(47.63%), P. expansum (19,05%), T. harzianum (9,53%), A. nidulans, A. niger, Penicillium sp.,Candida albicans, and T. vitrens i.e 4,76% respectively. All of rhizosphere fungi colonies were foundto inhibit Fusarium oxysporum f.sp. capsici in vitro. The best inhibition was found in Aspergillusniger at 88.89 ± 2.2% followed by A. nidulans of 85,56 ± 1,6 %, T. harzianum at 84,45 ± 1,58% , andT. virens by 83,33 ± 1,2%, five days after inoculation. All of them have a very high inhibition criteria.
MOLECULAR IDENTIFICATION OF EXOTIC FRUIT FLY Bactrocera occipitalis (DIPTERA: TEPHRITIDAE) USING MITOCHONDRIAL CYTOCHROME OXIDASE I (COI) GENE I Putu Sudiarta; Dwi Martiningsia; I Nyoman Wijaya
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5’-GGAGCATTAATYGGRGAYG-3’ as forward primer and HCO 5’-TAAACTTCAGGGTGACCAAAAATCA-3’ as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.
INCREASING LOCAL FRUITS COMPETITIVENESS IN ENTERING THE TOURISM MARKET IN BALI I Gede Putu Wirawan; Ketut Srie Marhaeni Julyasih; Wayan Adiartayasa; I Nyoman Wijaya; I Putu Anom
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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The study focused on improving the fruit quality supported by handling of the cultivation, harvest and post-­??harve st handling such as fruit packing, transportation, storage, and others, as well as marketing strategiesand regulations that are ne eded locally, so local Bali fruits can compete better in the Bali tourism market.The purposes of this study were to find some problems face by the local fruits to e nter the tourism marke t inBali. The local fruits studied were citrus, salacca, mangoes, mangosteen, banana, and papaya. This study wasthrough multiple surveys such as, inte rviews with farme rs, whole salers, fruit suppliers, and the manage rsof the hote ls and restaurants. The results of this study found some local fruits have e nte red the tourismmarket in Bali, in addition, local people in Bali te nd to consume the imported fruits for some culturalceremony, events and others.The results of this study showthat the main problems in the local fruits suchas citrus and banana were harvesting, packaging, storage, and transportation. The laboratory studies foundthat some microbes and inse cts infe cted local fruits due to the lackof handling such as packaging, storage,and transportation. The findings of this study was local fruits such as orange s and bananas face manyobstacles ranging from harvest, post-­??harvest management, transportation, and the marketing strategies.Many problem has found on bananas such as damaged on storage, as well as citrus fruits. Improvements tothe appearance of the fruit has been tried to do by the application of emulsion of he rbal oils, oleic acid, wax,twee n, and the results showe d the fruits still in a fre sh condition afte r four weeks of treatme nt while theuntre ated fruits were rotten due tothe infe ction of microbes. The blackspots of insects ormicrobes infectionson the treated fruits can be eliminated as well. Some inse cts and microbial attacks were found that leads tofruit rot which can essentially be removed by tre atment with antimicrobial tre atment. This research tries tochart the effects on the competitiveness of local fruits, such as continuity of production, standardization andregulation which could be developed at the local level in the village community.
PELATIHAN PENULISAN ARTIKEL ILMIAH PENGABDIAN KEPADA MASYARAKAT KOMANG BUDAARSA; NYOMAN WIJAYA
Buletin Udayana Mengabdi Vol 6 No 1 (2007): Volume 6 No.1 - April 2007
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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ASBSTRACT Training of writing of sience article of community services have been conducted on 20 October 2006 followed 24 people, from 10 faculty in Udayana University. Target of this training to give skill and knowledge to a number of lecturer specially young lecturer in Udayana University about technique sience article of community services. After training expected by all participant can make real correct and good sience article of community services as according to existing guidance. Training conducted in the form of tutorial for the theory of discussion and, later then continued with direct practice make sience article of community services. Before executed training of participant given pretest which contents concerning writing of sience article of community services. The Tes meant to know how far knowledge and understanding of participant before given items. Result of tes indicate that knowledge of participant before given training is 82 %. Later then after given training become 100 %. Its meaning happened the make-up of understanding and knowledge counted 18% from participant to items the given. Have succeeded to be made 19 title sience article of community services which have followed real correct writing method. The article will be given high priority to be to be loaded in journal of Udayana Mengabdi next edition.
PENDIDIKAN DAN PELATIHAN PENGENDALIAN TERPADU HAMA PENGGEREK BATANG PADI DI KELURAHAN PENATIH, KECAMATAN DENPASAR TIMUR, KOTA DENPASAR : I Nyoman Wijaya
Buletin Udayana Mengabdi Vol 7 No 1 (2008): Volume 7 No.1 – April 2008
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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ABSTRACT Education and training on integrated pest management (IPM) of Rice Stem Borrer has been held at Penatih village, Denpasar regency on 15 st September 2007. The activities aimed to improve knowledge farmer of Rice Stem Borrer and factors influence the intensity, and integrated pest management of Rice Stem Borrer. The topics covered were symptom, bioecology, insect distribution and integrated management of Rice Stem Borrer. The training was attended by 25 participants from local farmer groups of Penatih village. The method used in activities were lectures, demontration and practical in the rice field. All participants participited anthusiastically and hope the have the next intensive traning of the rice culture.
KARAKTERISTIK MOLEKULER DAN FILOGENI LALAT BUAH Bactrocera occipitalis (Diptera:Tephritidae) DARI TARAKAN BERDASARKAN SEKUEN NUKLEOTIDA GEN COI Dwi Martiningsia; I Nyoman Wijaya; I Putu Sudiarta
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Bactrocera occipitalis is one species of group B. dorsalis complex. The main host of B. occipitalis is mango (Mangifera indica), guava (Psidium guajava), orange (Citrus sp), sapodilla (Achras zapota) and star fruit (carambola Averrhoa). Region of distribution B. occipitalis are Philippines, East Malaysia (Sabah), Brunei, Indonesia (Kalimantan). B. dorsalis complex has similar morphological and have a less distinctive character for taxonomic identification at immature life stages, it will be an obstacle for the export of fruit and vegetables from Indonesia related to quarantine the country of destination, and DNA-based barcode can solve this problem. Molecular Identification B. occipitalis Tarakan successfully amplified at 642 bp with COI gene. Based on sequence homology COI B. occipitalis Tarakan has a high of similarity with B. occipitalis Philippines is 99.4% -100%. The phylogenetic analysis of DNA sequences and genetic distance based on COI gene sequences showing too genetic closeness with B. occipitalis Philippines.
Identifikasi Mikoriza Vesikular Arbuskular pada Rhizosfer Tanaman Lamtoro (Leucaena leucocephala) dan Kaliandra (Calliandra calothyrsus) serta Perbanyakannya dengan Media Zeolit NI WAYAN PUSPARINI DHARMAPUTRI; I NYOMAN WIJAYA; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Vesicular Arbuscular Mycorrhizae Identification of Lamtoro (Leucaena leucocephala) and Kaliandra (Calliandra calothyrsus) Rhizosphere and Its Spore Multiplication in Zeolit MediaSongan village was located in the district of Kintamani, Bangli. The plants population were dominated by lamtoro and kaliandra. This plant was included in the Fabaceae (Leguminoseae) family which capable of forming nodules and symbiotic association to mycorrhizae. This study aimed to determine the types mycorrhizal of rhizosphere lamtoro and kaliandra plants and determine the effectiveness of zeolite media and corn symbiont plant in propagation of VAM. The experiment was conducted from Desember 2014 to Februari 2015, in the Laboratory of Genetic Resources Unit and Molecular Biology University of Udayana. Spore isolation was done by conducting wet sieving method. Roots colonization percentages were calculated by root staining method and spores multiplication through trapping culture method. The results showed that VAM spores found in the rhizosphere lamtoro plants are three genera that resembled Acaulospora, Glomus, and Scutellospora whereas in the rhizosphere of kaliandra plants found two genera that resembled Acaulospora and Glomus. Mycorrhizal structures found in the rhizosphere of lamtoro and kaliandra plants were vesicles and inner spores.VAM from the rhizosphere of lamtoro and kaliandra plants can be reproduced using zeolite media and corn symbionts.
Deteksi Penyakit Citrus Vein Phloem Degeneration (CVPD) Dengan Teknik Polymerase Chain Reaction (PCR) pada Daun Tanaman Jeruk Yang Memiliki Pola Gejala Klorosis Berbeda RIDA MELANI; WAYAN ADIARTAYASA; I NYOMAN WIJAYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.2, April 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Citrus Vein Phloem Degeneration (CVPD) Disease Detection With Polymerase Chain Reaction (PCR) Technique In Citrus Plant Leaves That Have Different Chlorosis Symptom Patterns Detection of Citrus Vein Phloem Degeneration (CVPD) disease with Polymerase Chain Reaction (PCR) on citrus leaves that have different pattern of chlorosis symptoms was conducted at laboratory of Genetic Resources and Biology Molecular, Udayana University. This study aims to determine the presence of Liberibacter asiaticus in citrus leaves that have different patterns of chlorosis symptoms using a specific primer 16S rDNA (OI1 and OI2c). DNA amplified on 1% agarose gel obtained DNA bands of 1160 bp on four samples with a pattern of symptoms of chlorosis.
Uji Efektifitas Ekstrak Daun Sirih (Piper Betle L.),Kirinyuh (Chromoloena Odorata) dan Tembelekan (Lantana Camara L.) Terhadap Populasi Nematoda Puru Akar (Meloidogyne Spp.) dan Pertumbuhan Tanaman Cabai (Capsicum Annuum L) MAGNA DWIPAYANA; I NYOMAN WIJAYA; MADE SRITAMIN
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Effectiveness Test of Extracts Of Betel Leaf (Piper Betle L.), Kirinyuh (Chromoloena Odorata) and Tembelekan (Lantana Camara L.) Against The Population of Root Knot Nematodes (Meloidogyne Spp.) and Plant Growth of Chili (Capsicum Annuum L.)Meloidogyne spp. was one of the parasitic nematode pests on crops of chili. Meloidogyne spp. was widespread in tropical regions and subtropik area.. Attacks of Meloidogyne spp. at the root can lower the production of the crops by 15 – 60 percent, even can reach 70 percent when the disease attacks vulnerable plants (Prihanto,1989). This research was carried out in a way controlling root knot nematodes on chili uses extract of 3 species of plants in a variety of concentrations with the aim to obtain a concentration from the plants that are very effective at suppressing the development of root knot nematodes and can suppress the population in the soil and as well to surpress the attacks on plant roots.The types of plants that will be used in this research is kirinyuh (Chromolaena odorata), tembelekan (Lantana camara) and betel leaf (Piper betle) in different concentrations to know their ability to suppress populations of root knot nematodes of chili. The leaves of the plant contains useful materials to suppress plant pests including root knot nematodes. From the three of leaf extract that have been used, the betel leaf extract effectively suppress the populations of nematodes on crops of chili for being able to put pressure against the population of the nematode in the soil or plant roots of chili. At the concentrations of 50 cc/polybag betel extracts are able to suppress the nematode population to 93% and on the concentration of 200cc/ polybag betel extracts was able to suppress populations of nematodes were 96.4%.
Deteksi Penyakit Citrus Vein Phloem Degeneration (CVPD) dengan Teknik Polymerase Chain Reaction (PCR) pada Tanaman Jeruk di Bali NI PUTU SWARI MEITAYANI; WAYAN ADIARTAYASA; I NYOMAN WIJAYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.2, April 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACTS Detection of Citrus Vein Phloem Degeneratin (CVPD) Disease by Polymerase Chain Reaction (PCR) Technique on Citrus Plant in Bali CVPD disease is one of many important disease on citrus plant caused by Liberobacter asiaticum. The mechanism of infection is not yet known, so that the efforts to control the disease have not been adequately. In the research, we tried to detect CVPD disease on citrus plant at several locations in the centre of the citrus plantation in Bali used the PCR technique. The results of reseach showed eleven from eighteen sample is positive have contain for the L. asiaticum that show size of DNA 1160 bp, because the size of 1160 bp DNA bands is expression by L. asiaticum, then the citrus leaf samples were detected positive for the L. asiaticum. Based on the results of the calculation incidence of the disease with percentage of attack is 54,38% and the intensity of CVPD diseased plants was 9,86% showed CVPD disease was spread over eight villages consist of Katung, Belancan, Bayung Gede, Awan, Catur, Pengotan, Pelaga and Petang. Keyword: Citrus Vein Phloem Degeneration (CVPD), Liberobacter asiaticum, Polymerase Chain Reaction (PCR)
Co-Authors Abdul Rahem Abdul Rahem Abdul Rahem, Abdul Ade Febrianto Umbu Romu Kolambani AGNESIA KURNIAWATI Akhmad Khoirul Munir Aldrik Joe Melvin Tarigan Ana Yuda ANAK AGUNG GEDE GARBA YOGANTARA Andi Hermansyah Andi Hermansyah Antonius Gamma Wardana Antony Lay Aqila Dira Eka Maretha Ar Rayyan, Muhammad Zhafir Argya Arsy Isa Rajendra Arie Sulistyarini Arie Sulistyarini Athayya Indy Nasywa Aulia, Shasky Saffira Awahita, Bahira Aisyah Ayu Anggreini Sitohang Azzahro, Sabrina Aulia Bakar, Nor Kartini Abu Bambang Subakti Z Bambang Tri Purwanto Brandon Budiatin, Aniek Setiya Candrakirana, Sonya Arini Catur Dian Setiawan Cha, Joo Young Chrismawan Ardianto Costa Cruz, Latizio Beni Da Dalfa, Dela Daniel Emmanuel Wijaya Dewi Melani Hariyadi Diana Putri Lestari Dikta, I Putu Gede Asnawa Djoko Agus Purwanto Dwi Martiningsia Dwi Martiningsia DWI WIDANINGSIH Einarel Yusufrizky Wibisono Ekawati, Ni Enjelina, Ni Desak Gede Rista Fakhriatun Nisa Fany Zumrotul Faizah Farah Mahdiyyah Farhan Hanang Ichsanto Farida Ifadotunnikmah Fatmawati, Anjar Felisya, Anggi Ratu Gayatri, Anak Agung Sagung Intan GEDE SUASTIKA GEDE WIJANA Gesnita Nugraheni Giantari, I Gusti Ngurah Alit Susanta Wirya Gusti Noorrizka V Gusti Noorrizka Veronika Ahmad Hadi Poerwono Hamdri, Apta Amalia Handayani, Rosita Hanni Prihhastuti Puspitasari Hayuningtyas, Eka Putri Hermansyah, Andi Husni dan Zulfanazli Pudjianto Alfian Rusdy I Dewa Nyoman Nyana I Gde Antha Kasmawan I GEDE BAGUS DWIPANANDA I GEDE PUTU WIRAWAN I Gede Sumaradana I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Siadi I Ketut Suada I KETUT SUMIARTHA I Komang Budaarsa I Komang Widya Purnama Yasa I Made Gede Anadhi I Made Mega Adnyana I MADE SUDARMA I MADE SUDARMA I Nyoman Darma Putra I NYOMAN RAI I Nyoman Suarka I NYOMAN SUKIADA I Nyoman sukiada I Nyoman Wijaya I Putu Agus Hendra Wibawa I Putu Anom I Putu Eko Arymanto Wira Kesuma I Putu Sudiarta I Wayan Ardika I WAYAN SASTONO I Wayan Suardiana I Wayan Supartha Ida Ayu Dwi Giriantari Iman, Evelin Nicole Juni Ekowati Kamila, Azzihra Rahma KETUT AYU YULIADHI Ketut Srie Marhaeni Julyasih Lesmana, Anindya Nadine Liza Pristianty LUH TINA ARYANTINI M. Rahmad M. Rahmad, M. Rahmad MADE SRITAMIN Madona Dwi Fitriani MAGNA DWIPAYANA MAHARDHIKA DWI PUTRA WIJAYA Mahardian Rahmadi Mancika, Ni Made Juliana Dewi Marcellino Rudyanto Mardani, Widya Tama Melva Santi Sihaloho Mintari, Gusti Ayu Willem MISBAHUL KHULUQ MOCH BINTANG RAMADHAN Mohammad Hefni Hidayat Mufarrihah, Mufarrihah Muharrom Riezky Prasetyo Munthe, Lambok Jenny Sagita Nababan, Evan Gabriel Nathganeila, Luthfia Andini Naufal Athaullah Aulia Naufal Dhifari Ramadhan Nawal Karina Basalamah Nazwa Zahira Anwar NEVIATY PUTRI ZAMANI NI KETUT JULI DWI JAYANTI Ni Made Puspawati Ni Made Regina Prasetya Putri NI MADE TRIGUNASIH NI NENGAH DARMIATI NI NYOMAN ARI MAYADEWI NI PUTU SWARI MEITAYANI NI WAYAN ARMINI NI WAYAN PUSPARINI DHARMAPUTRI NI WAYAN SUNITI Ningsih, Gusti Ketut Asti Dea Nona Au Waling Noorma Rosita Noorrizka V, Gusti Norine Nitya Ardiningrum Novayolla, Alsha Nurftatmelia Nurfadzilah, Puteri Gisyela NURUL KHOTIMAH NYOMAN AYU TRISNA ARI UTAMI OCTA FRANSISCA SITORUS Oktaviani, Faradila Putri Parwata, I Made Oka Pranata, Kadek Dwipayana Priyandani, Yuni Purwitasari, Neny PUTU PADMA MAHESWARI Qanita Afrannajla Rafid Khairi Santoso Rahayu, Titik Puji Rahmani, Risma Sekar RAMADHAN, MOCH BINTANG RIDA MELANI Rika Uyunul Mabilla RISMA IMROATUS SHOLIHAH Sabila, Bilqis Rif'atus Safitri, Zelda Santoso, Aina Cahyaning Sasadara, Maria Malida Vernandes Siahaan, Enjelina Silfa Fajriyatur Rosyidah Siti Rahmah SRI LESTARI Sri Lestari Sugiarto, Jennifer Angeline Sugijanto Sugijanto Sukaatmadja, I Suko Hardjono Sukorini, Anila Impian Sumarno . Suzana Syalsabila Aisyah Rahmawati Tarsat, Mosli Tho'ati, Fitri Nur Hidayatuth Timothy Alessandro Christaluary Lanapu Titania Fiska Ornelia Toetik Aryani Tri Widiandani TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Tristiana Erawati Tuan, Nguyen Thanh Tutiek Purwanti Umi Athiyah Umi Athiyah, Umi Ursulla Yulananda Anugraheni UTAMA, I WAYAN EKA KARYA Vanessa Emas Permata Sari VANI SILVANA Vaturrachma, Amelia Safira Putri VLORA VERONICA SIPANGKAR Wahyu Utami Wahyu Utami Wahyu Utami, Wahyu Walidah, Ziyana Wardah Zuhan Nafikhah WAYAN ADIARTAYASA WIDHIANTHINI WIDHIANTHINI Widiarsana Putra, I Made Widji Soeratri Wigunanda, I Wayan Surya Aditya Wiraatmaja, Wayan Wiwied Ekasari Yogi, Ida Bagus Putu Prajna YUDHA, I KADEK WISMA Yuni Priyandani Yuni Priyandani Yunita Nita Yusuf, Helmy Zhao, Tiejun Zulkamain, Bambang Subakti Zulkarnain, Bambang Subakti