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All Journal Humaniora Agrotrop : Journal on Agriculture Science AGRITROP International Journal of Biosciences and Biotechnology HUMANIS Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology E-Journal Of Cultural Studies E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Paramita: Historical Studies Journal Jurnal Kajian Bali Berkala Kedokteran International Research Journal of Management, IT & Social Sciences IHiS (Indonesian Historical Studies) Journal of Maritime Studies and National Integration PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Entomologi Indonesia Jurnal Bali Membangun Bali Jurnal Insan Farmasi Indonesia Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Farmasi Komunitas Nandur Jurnal Pengabdian Masyarakat Ilmu Kesehatan
I NYOMAN WIJAYA
Jurusan/Prodi Agroekoteknologi Fakultas Pertanian Universitas Udayana
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UTILIZATION OF RHIZOSPHERE FUNGI TO CONTROL Fusarium oxysporum f.sp. capsici IN VITRO I Made Sudarma; Ni Made Puspawati; Ni Wayan Suniti; I Nyoman Wijaya; I Gusti Ngurah Bagus
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Fusarium caused wilt disease in chilli pepper and destroyed some farmer crops. Results ofpreliminary research has been discovered that the disease caused by Fusarium oxysporum f.sp. capsici.The alternative environmental friendly method is to find antagonist microbes which is located in therhizosphere of healthy pepper plants. This study aims to find out potentially antagonistic fungi tocontrol Fusarium wilt disease on pepper plants. The fungi were isolated by soil dilution method orviable plate count method on Potato Dextrose Agar medium with antibiotic Livoplaxacin (25%, w/v).Rhizosphere fungi from healthy pepper plants had been identified. A total of 63 spesies belong to 4genera included Penicillium (45 species), Aspergillus (6 species), Trichoderma (9 species) andCandida (3 species). The highest percentage of distribution of rhizosphere fungi are P. digitatum(47.63%), P. expansum (19,05%), T. harzianum (9,53%), A. nidulans, A. niger, Penicillium sp.,Candida albicans, and T. vitrens i.e 4,76% respectively. All of rhizosphere fungi colonies were foundto inhibit Fusarium oxysporum f.sp. capsici in vitro. The best inhibition was found in Aspergillusniger at 88.89 ± 2.2% followed by A. nidulans of 85,56 ± 1,6 %, T. harzianum at 84,45 ± 1,58% , andT. virens by 83,33 ± 1,2%, five days after inoculation. All of them have a very high inhibition criteria.
MOLECULAR IDENTIFICATION OF EXOTIC FRUIT FLY Bactrocera occipitalis (DIPTERA: TEPHRITIDAE) USING MITOCHONDRIAL CYTOCHROME OXIDASE I (COI) GENE I Putu Sudiarta; Dwi Martiningsia; I Nyoman Wijaya
International Journal of Biosciences and Biotechnology Vol 6 No 1 (2018)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.163 KB) | DOI: 10.24843/IJBB.2018.v06.i01.p03

Abstract

Some of fruit flies have been reported as the important pest on fruits and vegetables in the world. Agricultural Quarantine Agency Denpasar reported that there was new coming species (exotic) of fruit flies in Bali in 2014 based on the morphological identification, namely Bactrocera occipitalis. However Bactrocera dorsalis complex have similar morphological characters and have a less distinctive character for taxonomic identification, therefore it is difficult to identify fruit flies accurately. Based on that phenomena, the accurate identification is needed. One of the more accurate identification techniques is based on molecular identification using DNA-based barcode. To identify fruit flies, DNA-based barcode using mitochondrial cytochrome oxidase I (COI) gene has been conducted. PCR analysis using Fruit Fly MT-CO1-F (FFMT-CO1-F) 5’-GGAGCATTAATYGGRGAYG-3’ as forward primer and HCO 5’-TAAACTTCAGGGTGACCAAAAATCA-3’ as reverse primer was successfully amplified around 600 bp of COI gene of fruit flies. Based on similarity of sequence product, the species was identifiedas Bactrocera occipitalis and same result was revealed using morphological identification. Phylogenetic analysis of B. occipitalis based on COI genes showed that B. occipitalis from Bali were in the same groups with Bactrocera species from Tarakan and Philippines. In addition, Bactrocera occipitalis as exotic fruit fly is a new report in Bali, Indonesia.
INCREASING LOCAL FRUITS COMPETITIVENESS IN ENTERING THE TOURISM MARKET IN BALI I Gede Putu Wirawan; Ketut Srie Marhaeni Julyasih; Wayan Adiartayasa; I Nyoman Wijaya; I Putu Anom
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

The study focused on improving the fruit quality supported by handling of the cultivation, harvest and post-­??harve st handling such as fruit packing, transportation, storage, and others, as well as marketing strategiesand regulations that are ne eded locally, so local Bali fruits can compete better in the Bali tourism market.The purposes of this study were to find some problems face by the local fruits to e nter the tourism marke t inBali. The local fruits studied were citrus, salacca, mangoes, mangosteen, banana, and papaya. This study wasthrough multiple surveys such as, inte rviews with farme rs, whole salers, fruit suppliers, and the manage rsof the hote ls and restaurants. The results of this study found some local fruits have e nte red the tourismmarket in Bali, in addition, local people in Bali te nd to consume the imported fruits for some culturalceremony, events and others.The results of this study showthat the main problems in the local fruits suchas citrus and banana were harvesting, packaging, storage, and transportation. The laboratory studies foundthat some microbes and inse cts infe cted local fruits due to the lackof handling such as packaging, storage,and transportation. The findings of this study was local fruits such as orange s and bananas face manyobstacles ranging from harvest, post-­??harvest management, transportation, and the marketing strategies.Many problem has found on bananas such as damaged on storage, as well as citrus fruits. Improvements tothe appearance of the fruit has been tried to do by the application of emulsion of he rbal oils, oleic acid, wax,twee n, and the results showe d the fruits still in a fre sh condition afte r four weeks of treatme nt while theuntre ated fruits were rotten due tothe infe ction of microbes. The blackspots of insects ormicrobes infectionson the treated fruits can be eliminated as well. Some inse cts and microbial attacks were found that leads tofruit rot which can essentially be removed by tre atment with antimicrobial tre atment. This research tries tochart the effects on the competitiveness of local fruits, such as continuity of production, standardization andregulation which could be developed at the local level in the village community.
Medan Perjuangan Sosial Intelektual Hindu Bali Dalam Menghadapi Tantangan Islam Politik Kementerian Agama Republik Indonesia 1951-1958 Melva Santi Sihaloho; I Nyoman Wijaya
Humanis Vol 21 No 1 (2017)
Publisher : Udayana University

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Abstract

Through the use of Social Struggle Field Boerdieu this study will not only talk about the position of Hindu Balinese intellectual groups. It includes discussing their tendency to reproduce, reshaping the Balinese social order in the face of the Islamic political group of the Ministry of Religious Affairs of the Republic of Indonesia (KAGRI). There are three research questions posed in this study, whose answers are sought by using the methodology of the political history, social history, and intellectual history of Kuntowijoyo’s. Besides, it is also used to assist the critical social science theory of poststructuralism, especially the generative structural theory of Boerdieu. The theory is used as the basis for thinking in digging and reconstructing the data and also placed within the framework of post-structuralism approach of Boerdieu style. In this way, the study was able to produce three conclusions from each of the research questions, which were then raised into a quintessence of conclusion that, in reproducing the social fabric of Balinese society in the face of the political Islamist group of the Ministry of Religious Affairs of the Republic of Indonesia (KAGRI) Balinese Hindu intellectuals put forward cultural thinking rather than economic thinking. Cultural thinking evolved from the habitus they had acquired since childhood. In facing the challenge of KAGRI political Islam group they are discouraging cultural thinking to the print media.
Biografi Rakyat Kecil, Sutikno: 1980-2018 Pendekatan Sejarah Post-Strukturalisme Akhmad Khoirul Munir; I Nyoman Wijaya; I Nyoman Sukiada
Humanis Vol 24 No 2 (2020)
Publisher : Udayana University

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Abstract

According to Kuntowijoyo writing biography can also be implemented against a person who comes from the unknown or participants. On that basis, study this takes up figure named Sutikno, a seacoast origin Bayuwangi East Java which currently domicile in Denpasar Bali by work as a mechanic motorcycle. A profession Sutikno will be the entrance in analysis, with an emphasis how he was that of a migrant (seacoast) generally in Bali can work as a mechanic workshop motorcycles with income that relatively success. By wearing a method/methodology Genealogi and theories post-strukturlism, especially the undirected Structural Generative Pierre Bourdieu and the Discourse-(Knowledge-Power )-Discipline Michel Foucault as a thought, the three had obtained. drawing conclusions. 1) With hard work and discipline, patient and keep trying not to despair Sutikno managed to achieve success even though through various obstacles and problems. 2) Factors behind the success of Sutikno mainly came from habitus and discipline and social private ownership of capital as a replacement for other capital. 3) Implication after sutikno pillars of success, that is a change from a social lower middle middle-class process for social climbed the stairs, characterized by differentiation consumption of goods that more luxurious and diverse than ever before.
PELATIHAN PENULISAN ARTIKEL ILMIAH PENGABDIAN KEPADA MASYARAKAT KOMANG BUDAARSA; NYOMAN WIJAYA
Buletin Udayana Mengabdi Vol 6 No 1 (2007): Volume 6 No.1 - April 2007
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

ASBSTRACT Training of writing of sience article of community services have been conducted on 20 October 2006 followed 24 people, from 10 faculty in Udayana University. Target of this training to give skill and knowledge to a number of lecturer specially young lecturer in Udayana University about technique sience article of community services. After training expected by all participant can make real correct and good sience article of community services as according to existing guidance. Training conducted in the form of tutorial for the theory of discussion and, later then continued with direct practice make sience article of community services. Before executed training of participant given pretest which contents concerning writing of sience article of community services. The Tes meant to know how far knowledge and understanding of participant before given items. Result of tes indicate that knowledge of participant before given training is 82 %. Later then after given training become 100 %. Its meaning happened the make-up of understanding and knowledge counted 18% from participant to items the given. Have succeeded to be made 19 title sience article of community services which have followed real correct writing method. The article will be given high priority to be to be loaded in journal of Udayana Mengabdi next edition.
PENDIDIKAN DAN PELATIHAN PENGENDALIAN TERPADU HAMA PENGGEREK BATANG PADI DI KELURAHAN PENATIH, KECAMATAN DENPASAR TIMUR, KOTA DENPASAR : I Nyoman Wijaya
Buletin Udayana Mengabdi Vol 7 No 1 (2008): Volume 7 No.1 – April 2008
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

ABSTRACT Education and training on integrated pest management (IPM) of Rice Stem Borrer has been held at Penatih village, Denpasar regency on 15 st September 2007. The activities aimed to improve knowledge farmer of Rice Stem Borrer and factors influence the intensity, and integrated pest management of Rice Stem Borrer. The topics covered were symptom, bioecology, insect distribution and integrated management of Rice Stem Borrer. The training was attended by 25 participants from local farmer groups of Penatih village. The method used in activities were lectures, demontration and practical in the rice field. All participants participited anthusiastically and hope the have the next intensive traning of the rice culture.
KARAKTERISTIK MOLEKULER DAN FILOGENI LALAT BUAH Bactrocera occipitalis (Diptera:Tephritidae) DARI TARAKAN BERDASARKAN SEKUEN NUKLEOTIDA GEN COI Dwi Martiningsia; I Nyoman Wijaya; I Putu Sudiarta
Journal of Agricultural Science and Biotechnology Vol 6 No 1 (2017)
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Bactrocera occipitalis is one species of group B. dorsalis complex. The main host of B. occipitalis is mango (Mangifera indica), guava (Psidium guajava), orange (Citrus sp), sapodilla (Achras zapota) and star fruit (carambola Averrhoa). Region of distribution B. occipitalis are Philippines, East Malaysia (Sabah), Brunei, Indonesia (Kalimantan). B. dorsalis complex has similar morphological and have a less distinctive character for taxonomic identification at immature life stages, it will be an obstacle for the export of fruit and vegetables from Indonesia related to quarantine the country of destination, and DNA-based barcode can solve this problem. Molecular Identification B. occipitalis Tarakan successfully amplified at 642 bp with COI gene. Based on sequence homology COI B. occipitalis Tarakan has a high of similarity with B. occipitalis Philippines is 99.4% -100%. The phylogenetic analysis of DNA sequences and genetic distance based on COI gene sequences showing too genetic closeness with B. occipitalis Philippines.
Identifikasi Mikoriza Vesikular Arbuskular pada Rhizosfer Tanaman Lamtoro (Leucaena leucocephala) dan Kaliandra (Calliandra calothyrsus) serta Perbanyakannya dengan Media Zeolit NI WAYAN PUSPARINI DHARMAPUTRI; I NYOMAN WIJAYA; WAYAN ADIARTAYASA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Vesicular Arbuscular Mycorrhizae Identification of Lamtoro (Leucaena leucocephala) and Kaliandra (Calliandra calothyrsus) Rhizosphere and Its Spore Multiplication in Zeolit MediaSongan village was located in the district of Kintamani, Bangli. The plants population were dominated by lamtoro and kaliandra. This plant was included in the Fabaceae (Leguminoseae) family which capable of forming nodules and symbiotic association to mycorrhizae. This study aimed to determine the types mycorrhizal of rhizosphere lamtoro and kaliandra plants and determine the effectiveness of zeolite media and corn symbiont plant in propagation of VAM. The experiment was conducted from Desember 2014 to Februari 2015, in the Laboratory of Genetic Resources Unit and Molecular Biology University of Udayana. Spore isolation was done by conducting wet sieving method. Roots colonization percentages were calculated by root staining method and spores multiplication through trapping culture method. The results showed that VAM spores found in the rhizosphere lamtoro plants are three genera that resembled Acaulospora, Glomus, and Scutellospora whereas in the rhizosphere of kaliandra plants found two genera that resembled Acaulospora and Glomus. Mycorrhizal structures found in the rhizosphere of lamtoro and kaliandra plants were vesicles and inner spores.VAM from the rhizosphere of lamtoro and kaliandra plants can be reproduced using zeolite media and corn symbionts.
Deteksi Penyakit Citrus Vein Phloem Degeneration (CVPD) Dengan Teknik Polymerase Chain Reaction (PCR) pada Daun Tanaman Jeruk Yang Memiliki Pola Gejala Klorosis Berbeda RIDA MELANI; WAYAN ADIARTAYASA; I NYOMAN WIJAYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.2, April 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Citrus Vein Phloem Degeneration (CVPD) Disease Detection With Polymerase Chain Reaction (PCR) Technique In Citrus Plant Leaves That Have Different Chlorosis Symptom Patterns Detection of Citrus Vein Phloem Degeneration (CVPD) disease with Polymerase Chain Reaction (PCR) on citrus leaves that have different pattern of chlorosis symptoms was conducted at laboratory of Genetic Resources and Biology Molecular, Udayana University. This study aims to determine the presence of Liberibacter asiaticus in citrus leaves that have different patterns of chlorosis symptoms using a specific primer 16S rDNA (OI1 and OI2c). DNA amplified on 1% agarose gel obtained DNA bands of 1160 bp on four samples with a pattern of symptoms of chlorosis.
Co-Authors Abdul Rahem Abdul Rahem, Abdul Ade Febrianto Umbu Romu Kolambani AGNESIA KURNIAWATI Akhmad Khoirul Munir Ana Yuda ANAK AGUNG GEDE GARBA YOGANTARA Andi Hermansyah Antonius Gamma Wardana Antony Lay Ar Rayyan, Muhammad Zhafir Arie Sulistyarini Awahita, Bahira Aisyah Azzahro, Sabrina Aulia Bakar, Nor Kartini Abu Bambang Tri Purwanto Budiatin, Aniek Setiya Cha, Joo Young Chrismawan Ardianto Costa Cruz, Latizio Beni Da Dalfa, Dela Dewi Melani Hariyadi Dwi Martiningsia Dwi Martiningsia DWI WIDANINGSIH Enjelina, Ni Desak Gede Rista Fakhriatun Nisa Fany Zumrotul Faizah Farah Mahdiyyah Farida Ifadotunnikmah Fatmawati, Anjar GEDE SUASTIKA GEDE WIJANA Gesnita Nugraheni Gusti Ngurah Alit Susanta Wirya Gusti Noorrizka Veronika Ahmad Hamdri, Apta Amalia Handayani, Rosita Hayuningtyas, Eka Putri Hermansyah, Andi Husni dan Zulfanazli Pudjianto Alfian Rusdy I Dewa Nyoman Nyana I Gde Antha Kasmawan I GEDE BAGUS DWIPANANDA I GEDE PUTU WIRAWAN I Gede Sumaradana I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Siadi I Ketut Suada I KETUT SUMIARTHA I Komang Budaarsa I Made Gede Anadhi I Made Mega Adnyana I MADE SUDARMA I MADE SUDARMA I NYOMAN RAI I Nyoman Suarka I NYOMAN SUKIADA I Nyoman sukiada I Nyoman Wijaya I Putu Agus Hendra Wibawa I Putu Anom I Putu Eko Arymanto Wira Kesuma I Putu Sudiarta I Wayan Ardika I WAYAN SASTONO I Wayan Supartha Ida Ayu Dwi Giriantari Juni Ekowati KETUT AYU YULIADHI Ketut Srie Marhaeni Julyasih Lesmana, Anindya Nadine Liza Pristianty LUH TINA ARYANTINI MADE SRITAMIN MAGNA DWIPAYANA MAHARDHIKA DWI PUTRA WIJAYA Mahardian Rahmadi Mancika, Ni Made Juliana Dewi Melva Santi Sihaloho Mintari, Gusti Ayu Willem MISBAHUL KHULUQ MOCH BINTANG RAMADHAN Mohammad Hefni Hidayat Muharrom Riezky Prasetyo Nababan, Evan Gabriel Naufal Dhifari Ramadhan NEVIATY PUTRI ZAMANI NI KETUT JULI DWI JAYANTI Ni Made Puspawati NI MADE TRIGUNASIH NI NENGAH DARMIATI NI PUTU SWARI MEITAYANI NI WAYAN ARMINI NI WAYAN PUSPARINI DHARMAPUTRI NI WAYAN SUNITI Nona Au Waling Noorrizka V, Gusti Novayolla, Alsha Nurftatmelia NURUL KHOTIMAH NYOMAN AYU TRISNA ARI UTAMI OCTA FRANSISCA SITORUS Pranata, Kadek Dwipayana Priyandani, Yuni PUTU PADMA MAHESWARI Rahayu, Titik Puji RAMADHAN, MOCH BINTANG RIDA MELANI Rika Uyunul Mabilla RISMA IMROATUS SHOLIHAH Sabila, Bilqis Rif'atus Siti Rahmah Sri Lestari SRI LESTARI Sugiarto, Jennifer Angeline Sugijanto Sugijanto Suko Hardjono Sukorini, Anila Impian Sumarno . Suzana Tarsat, Mosli Titania Fiska Ornelia Toetik Aryani Tri Widiandani TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Tuan, Nguyen Thanh Umi Athiyah Umi Athiyah, Umi Ursulla Yulananda Anugraheni UTAMA, I WAYAN EKA KARYA VANI SILVANA VLORA VERONICA SIPANGKAR Wahyu Utami Wahyu Utami, Wahyu Walidah, Ziyana Wardah Zuhan Nafikhah WAYAN ADIARTAYASA WIDHIANTHINI WIDHIANTHINI Wiraatmaja, Wayan Wiwied Ekasari Yuda, Ana YUDHA, I KADEK WISMA Yuni Priyandani Zhao, Tiejun Zulkamain, Bambang Subakti Zulkarnain, Bambang Subakti