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Kultur Jaringan Jeruk Keprok Tejakula (Citrus reticulata var. Tejakula) Menggunakan Tunas Muda dan Biji Serta Deteksi CVPD dengan Teknik Polymerase Chain Reaction (PCR) VLORA VERONICA SIPANGKAR; I NYOMAN WIJAYA; MADE SRITAMIN
Agrotrop : Journal on Agriculture Science Vol 10 No 1 (2020)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.488 KB) | DOI: 10.24843/AJoAS.2020.v10.i01.p06

Abstract

Tissue Culture Citrus (Citrus reticulata var. Tejakula) using Shoot and Seed and the Detection of CVPD with Polymerase Chain Reaction (PCR). The sample was taken in Pecatu Village, Kuta Selatan District, Badung Regency and continued with in vitro culture and PCR analysis at UPT. Genetic Resource and Molecular Biology Laboratory, was start from December of 2018 until May of 2019. The purpose of this research was to create a CVPD free Citrus reticulata var. Tejakula seedling from tissue culture. The sample was chosen based on visual characteristic. Two samples were taken from citrus plant that show CVPD symptoms and citrus plant that did not show CVPD symptoms. The seed and shoot were cultured using MS media for 12 week after planting (WAP). From this research showed that both explant from the shoot and seed were able to grow well. The explant from citrus sample that showed CVPD symptoms was infected by Liberibacter asiaticus and the explants from citrus that did not show CVPD symptoms was not invected by Liberibacter asiaticus, according to PCR analysis.
Serangan Penggerek Batang Padi dan Peran Musuh Alami dalam Mengendalikan Populasinya pada Persawahan Tanam Serentak dan Tidak Serentak WAYAN ADIARTAYASA; I NYOMAN WIJAYA
Agrotrop : Journal on Agriculture Science Vol 6 No 1 (2016)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Paddy Rice Stemborer Attact and The Role of Parasitoid in Control That Population atSimultaneous and Stagered Ricefield. The arrangement of suistable agriculture in Baliwhich appropriate with Bali governments mission : Bali Clean and Green Go to Organic. Theconcept of modern agriculture look like contradiction with the aspects suistainableagriculture. The aspect of cultivation have been done by local agriculture community in Balicall it Subak for many years, but the attack of yellow rice borer still happen, wich adversefarmers side, persist. The efforts control wich has been done, still rely on pesticides, becauseit can give quick effect, but it is not appropriate for suistainable agriculture. The othersolution to solve that issues with the integrated pest management (IPM) as the maincomponent of with is the role parasitoids. The experiment was carried out at simultaneousplanting area (Subak Sidakarya, Denpasar Selatan District) and at staggered planting area(Subak Buaji, Denpasar Timur District). The result of this experiment showed that damageintensity of rice stemborer at simmultaneus planting was lower than at staggered plantingarea. There are four species stemborer at simultaneous planting area and at staggered plantingareas, namely Scirpophaga incertulas, Sesamia inferens, Chilo suppressalis, and Chilopolychrysus. Three kinds of egg parasitoids were found to decrease the population of ricedstemborer. They were Tetrastichus schoenobii, Telenomus rowani and Trichogrammajaponicum.
Uji Pemangsaan dan Tanggap Fungsional Predator Chysoperla carnea Stephens (Neuroptera: Crysopidae) Terhadap Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) I WAYAN DIRGAYANA; I WAYAN SUPARTHA; I NYOMAN WIJAYA
Agrotrop : Journal on Agriculture Science Vol 11 No 1 (2021)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2021.v11.i01.p08

Abstract

Predation and Functional Response Test of Predator Chysoperla carnea Stephens (Neuroptera: Crysopidae) Against Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae). This study aims to evaluate the predatory capacity of C. carnea by measuring the rate of searching capacity and handling-time of one prey and its functional response to the population density of P. manihoti. The research was conducted at the Integrated Pest Management Laboratory (IPMLab), Faculty of Agriculture, Udayana University. The study was conducted from February to May 2019. Testing of functional responses used a randomized block design with 5 treatments (3, 6, 9, 12, 15 nymphs-3) each of which was repeated 10 times. The results showed that the prey searching-capacity when the population was low (3 nymphs-3) took longer (10.37 minutes), while when the population was high it took a short time (6.23 minutes). The length of time for handling one prey in the low population was 6.08 minutes, while in the high population it was 5.48 minutes. Predator C. carnea has a tpe-2 functional response to an increase in the population of P. manihoti nymphs with the equation Y = 4.32x / 1 + 1.973x (R2 = 0.980). The rate of predation increases sharply when the population of low increases, and decreases when the increase of prey population increases. C. carnea has the potential to be developed as a control agent for P. manihoti.
Uji Efektivitas Beberapa Konsentrasi Ekstrak Daun Kirinyuh (Chromolaena odorata L.) Terhadap Perkembangan Ulat Krop Kubis (Crocidolomia pavonana F.) I NYOMAN WIJAYA; I GEDE PUTU WIRAWAN; WAYAN ADIARTAYASA
Agrotrop : Journal on Agriculture Science Vol 8 No 1 (2018)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.35 KB) | DOI: 10.24843/AJoAS.2018.v08.i01.p02

Abstract

Effectiveness Test of Several Concentrations of Kirinyuh Leaf Extract (Chromolaena odorata L.) Toward Development of Cabbage Crop Caterpillar (Crocidolomia pavonana F.). This research was conducted at the Laboratory of Plant Pests and Diseases of the Faculty of Agriculture, Udayana University from April 2018 to August 2018 to test the extract of kirinyuh leaves (Chromolaena odorata) on the development of biology of cabbage crop caterpillar (Crocidolomia pavonana). The completely randomized design (CRD) was used in this study, with four types of treatment of leaf extract concentration of 10%, 20%, 30%, 40%, and control. Each treatment was repeated 10 times. Observations were completed every day of the larval stage until the larvae do not carry out any activity anymore (dead), or until they become imago. The results showed that (1) the leaf extract of C. odorata plant which was used as research material had the potential to suppress the biological development of C. pavonanadi larvae in the laboratory, (2) the application of leaf extract concentration of 10% and 20% had an influence on the development of larvae which had developed slow compared to concentrations of 30% and 40%, where larvae experience rapid development of only 9 observations and (3) the most effective test leaf extract treatment was the treatment of leaf extracts of C. odorata concentrations of 40% and 30% followed by leaf extract concentrations of 20% and 10%.
Komposisi Spesies Penggerek Batang Padi dan Parasitoid Telurnya pada Ketinggian Tempat yang Berbeda I NYOMAN WIJAYA; WAYAN ADIARTAYASA; KETUT AYU YULIADHI
Agrotrop : Journal on Agriculture Science Vol 11 No 1 (2021)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2021.v11.i01.p01

Abstract

Composition of Rice Stem Borer Species and Their Egg Parasitoids at Different Altitudes. Rice stem borer is one of the main pest of rice plants that always percolating and attack plants in the field. The pests can result in damage to plants and lost of the grain from season to season. The aim of this research is to know composition and dominance rice stem borer species and role of their eggs parasitoid at an altitude of different places. Research is done by survey based on the height of different altitude in rice plants two weeks after cropping up until eleven weeks after planting. Research conducted from April until August 2019 in rice field that belonging to farmers at Subak Cemagi Let, Cemagi Village and Subak Teba, Mengwi village, Badung regency, Bali province. Subak Cemagi Let are at an altitude of 15 meters above sea level whereas Subak Teba are at an altitude of 300 meters above sea level. Identification of Rice stem borer larvae and their eggs parasitoid done in Pest Laboratory, Agriculture Faculty of Udayana University. Found three species rice stem borer in the height 15 mdpl namely Scirpophaga incertulas; Sesamia inferens dan Chilo suppressalis, whereas in the height 300 mdpl found four species namely Scirpophaga incertulas, Sesamia inferens, Chilo suppressalis, dan Chilo polychrysus. S. incertulas was the most dominant at the two different altitude. Three species of eggs parasitoid that have role to suppress the development of rice stem borer at the different altitude namely Tetrastichus schoenobii, Telenomus rowani dan Trichogramma japonicum. T. schoenobii was the most dominant to suppress rice stem borer populations.
The Knowledge of Lombok Praya-Indonesia Regional Community on the Safety Use of Medicinal Plants Wiwied Ekasari; Suko Hardjono; Sugijanto Sugijanto; Juni Ekowati; I Nyoman Wijaya; Tri Widiandani
Berkala Kedokteran Vol 17, No 2 (2021)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.127 KB) | DOI: 10.20527/jbk.v17i2.11671

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Abstract: The use of medicinal plants to maintain or overcome health problems has long been carried out by worldwide community. Along with the development of knowledge, data about the existence of undesirable effects or side effects associated with the use of medicinal plants that are not appropriate are obtained. This research is conducted to determine public knowledge about the safety several medicinal plants use. The study was conducted with a questionnaire technique in Lombok Praya, Indonesia. The results show that knowledge about the safety of the use of medicinal plants in breastfeeding mothers obtains the correct answer by 48%, knowledge about the parts of medicinal plants that are dangerous for consumption by 39% and knowledge about medicinal plants that can increase health risks by 21%. Whereas, the knowledges for the use of medicinal plants in pregnant women and the use of medicinal plants that are not right obtains very good results with correct answers of 79% and 81%. Based on further interviews, these good results are obtained because they are supported more by traditional use of the plant that has already existed in the area. Public understanding of the safety of some medicinal plants in Lombok Praya is still lacking. It is necessary to add knowledge about the use of medicinal plants that requires special attention, especially on plants that are often used by people in the area. Local health agencies need to disseminate information on the safety of medicinal plants that is wider and more sustainable. Keywords: Community, medicinal plant, safety, public understanding.
Dinamika Populasi Diaphorina citri Kuwayama (Homoptera: Psyllidae) dan Deteksi CVPD dengan Teknik PCR I NYOMAN WIJAYA; WAYAN ADIARTAYASA; MADE SRITAMIN; KETUT AYU YULIADHI
Jurnal Entomologi Indonesia Vol 7 No 2 (2010): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.745 KB) | DOI: 10.5994/jei.7.2.78

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The research was conducted in the field of Taro village Gianyar regency and Katung village Bangli regency and at the Laboratory of Biotechnology Faculty of Agricultural Udayana University. The purpose of the research were to investigate population dynamic of Diaphorina citri Kuwayama and other insects that were assosiated with citrus cv. siam; and molecular detection of CVPD patogen in its vector, other phytophagous insects on citrus cv.siam and kemuning [Murraya paniculata (L.) Jack.] The result of this study showed that in six month period there was one peak of D. citri population which was associated with the flushing period of citrus at Taro as well Katung villages. The peak of D. citri population of Taro village was higher than that of Katung village. Two species of parasitoids and four spesies of predators were found in the field and may contribute in the suppression of D. citri population. The parasitoids identified were Tamarixia radiata Wat. and Diaphorencyrtus alligharensi Shaffe, whereas the predators were Curinus coeruleus Mulsant, Phiddipus sp., Menochilus sexmaculatus Fabricius and Oxyopes javanus Thorell. T. radiata has been proven to be an effective nymph parasitoid that could suppress the population development of D. citri in citrus orchads. The research revealed that kemuning could serve as a source of infection because it is also a host of CVPD disease as well as of D. citri. Toxoptera citricidus Kirkaldy and Maleuterpes dentipes Hell. were not proven to contain CVPD disease.
Isolasi dan Skrining Bakteri Selulolitik pada Feses Luwak I Putu Eko Arymanto Wira Kesuma; I Nyoman Wijaya; Made Sritamin
Nandur Vol 1 No 3 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Civet (Paradoxurus hermaphroditus) are carnivorous animals, but also often eat fruit and one of them is coffee cherries. The coffee cherries that are eaten are not completely digested, so the coffee beans come out with the civet feces. In the civet digestion there are various kinds of bacteria that help the digestive process, one of them is cellulolytic bacteria. It is possible that coffee beans in civet feces contain cellulolytic bacteria.This study aims to determine the presence of cellulolytic bacteria in civet feces. The results of bacterial isolation in civet feces obtained 6 isolates that had different morphological characters. Cellulolytic bacteria screening results showed that 2 out of 6 isolates were positive as cellulolytic bacteria, namely FL2 isolate with a cellulolytic index of 2.38 (high reaction) and FL3 isolate with a cellulolytic index of 1.24 (medium reaction). The isolates FL2 and FL3 had different morphological characters in colony shape, margins and color, but had similarities in elevation, gram color and cell shape.
Identifikasi Senyawa Fitokimia pada Daun Kersen (Muntingia calabura Linn.) dan Uji Toksisitasnya Terhadap Larva Udang Artemia salina Leach Ade Febrianto Umbu Romu Kolambani; Made Sritamin; I Nyoman Wijaya
Nandur Vol 1 No 4 (2021)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Singapore cherry (Muntingia calabura L.) is a plant that has shady trees, is often used as a shade and is easy to grow in various places. All parts of the cherry plant, including roots, bark, fruit, and leaves, contain secondary metabolites that can be used as traditional medicine. The purpose of this study was to determine the phytochemical compounds contained in cherry leaf extract and to determine the level of acute toxicity (LC50) using the Brine Shrimp Lethality Test (BSLT) method. Identification of phytochemical compounds was carried out by gas chromatography-mass spectrometry and acute toxicity test (LC50) was carried out using the BSLT method using the shrimp larvae test insect Artemia salina Leach. The acute toxicity test (LC50) consisted of 6 concentration treatments, namely 500 ppm, 250 ppm, 150 ppm, 100 ppm, 50 ppm, along with negative controls, each of which was repeated three times. The LC50 value is obtained from probit analysis in Microsoft Office Excel. There are 3 groups of bioactive compounds with the highest peak area in singapore cherry leaf extract, namely groups of flavonoids, phenols, and alkaloid. The result of acute toxicity test (LC50) of singapore cherry leaves extract was 84,029 ppm.
Identifikasi Senyawa Fitokimia Kulit Batang Kelor (Moringa oleifera Lamk) Dengan Metode GC-MS dan Uji Toksisitas Terhadap Larva Udang Artemia salina leach Antony Lay; I Gede Putu Wirawan; I Nyoman Wijaya
Nandur Vol 2 No 1 (2022)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Kelor (Moringa oleifera) is a plant that grows in tropical climates including Indonesia. All parts of this plant can be used as medicine and other natural products. The purpose of this study was to determine the bioactive compounds contained in moringa stem bark and the level of toxicity expressed by an LC50 value of less than 1.000 ppm so that it can be used as traditional medicine and other natural products. Identification of bioactive compounds in moringa stem bark using the GC-MS analysis method and toxicity test on Artemia salina Leach shrimp larvae using the Brine Shrimp Lethality Test (BSLT) method. Based on the results of the analysis using GC-MS showed that moringa bark extract contains 14 bioactive compounds. The highest bioactive compounds in the 3 peaks were secondary metabolites in the form of phenols and monoterpenoids. The toxicity test of moringa bark extract against Artemia salina Leach larvae showed that the higher the concentration, the higher the percentage of mortality of Artemia salina Leach, so that the calculated LC50 value of moringa bark extract was toxic, which was 149,689 ppm.
Co-Authors Abdul Rahem Abdul Rahem Abdul Rahem, Abdul Ade Febrianto Umbu Romu Kolambani AGNESIA KURNIAWATI Akhmad Khoirul Munir Aldrik Joe Melvin Tarigan Ana Yuda ANAK AGUNG GEDE GARBA YOGANTARA Andi Hermansyah Andi Hermansyah Antonius Gamma Wardana Antony Lay Aqila Dira Eka Maretha Ar Rayyan, Muhammad Zhafir Argya Arsy Isa Rajendra Arie Sulistyarini Arie Sulistyarini Athayya Indy Nasywa Aulia, Shasky Saffira Awahita, Bahira Aisyah Ayu Anggreini Sitohang Azzahro, Sabrina Aulia Bakar, Nor Kartini Abu Bambang Subakti Z Bambang Tri Purwanto Brandon Budiatin, Aniek Setiya Candrakirana, Sonya Arini Catur Dian Setiawan Cha, Joo Young Chrismawan Ardianto Costa Cruz, Latizio Beni Da Dalfa, Dela Daniel Emmanuel Wijaya Dewi Melani Hariyadi Diana Putri Lestari Dikta, I Putu Gede Asnawa Djoko Agus Purwanto Dwi Martiningsia Dwi Martiningsia DWI WIDANINGSIH Einarel Yusufrizky Wibisono Ekawati, Ni Enjelina, Ni Desak Gede Rista Fakhriatun Nisa Fany Zumrotul Faizah Farah Mahdiyyah Farhan Hanang Ichsanto Farida Ifadotunnikmah Fatmawati, Anjar Felisya, Anggi Ratu Gayatri, Anak Agung Sagung Intan GEDE SUASTIKA GEDE WIJANA Gesnita Nugraheni Giantari, I Gusti Ngurah Alit Susanta Wirya Gusti Noorrizka V Gusti Noorrizka Veronika Ahmad Hadi Poerwono Hamdri, Apta Amalia Handayani, Rosita Hanni Prihhastuti Puspitasari Hayuningtyas, Eka Putri Hermansyah, Andi Husni dan Zulfanazli Pudjianto Alfian Rusdy I Dewa Nyoman Nyana I Gde Antha Kasmawan I GEDE BAGUS DWIPANANDA I GEDE PUTU WIRAWAN I Gede Sumaradana I Gusti Ayu Diah Yuniti I Gusti Ngurah Bagus I Ketut Siadi I Ketut Suada I KETUT SUMIARTHA I Komang Budaarsa I Komang Widya Purnama Yasa I Made Gede Anadhi I Made Mega Adnyana I MADE SUDARMA I MADE SUDARMA I Nyoman Darma Putra I NYOMAN RAI I Nyoman Suarka I NYOMAN SUKIADA I Nyoman sukiada I Nyoman Wijaya I Putu Agus Hendra Wibawa I Putu Anom I Putu Eko Arymanto Wira Kesuma I Putu Sudiarta I Wayan Ardika I WAYAN SASTONO I Wayan Suardiana I Wayan Supartha Ida Ayu Dwi Giriantari Iman, Evelin Nicole Juni Ekowati Kamila, Azzihra Rahma KETUT AYU YULIADHI Ketut Srie Marhaeni Julyasih Lesmana, Anindya Nadine Liza Pristianty LUH TINA ARYANTINI M. Rahmad M. Rahmad, M. Rahmad MADE SRITAMIN Madona Dwi Fitriani MAGNA DWIPAYANA MAHARDHIKA DWI PUTRA WIJAYA Mahardian Rahmadi Mancika, Ni Made Juliana Dewi Marcellino Rudyanto Mardani, Widya Tama Melva Santi Sihaloho Mintari, Gusti Ayu Willem MISBAHUL KHULUQ MOCH BINTANG RAMADHAN Mohammad Hefni Hidayat Mufarrihah, Mufarrihah Muharrom Riezky Prasetyo Munthe, Lambok Jenny Sagita Nababan, Evan Gabriel Nathganeila, Luthfia Andini Naufal Athaullah Aulia Naufal Dhifari Ramadhan Nawal Karina Basalamah Nazwa Zahira Anwar NEVIATY PUTRI ZAMANI NI KETUT JULI DWI JAYANTI Ni Made Puspawati Ni Made Regina Prasetya Putri NI MADE TRIGUNASIH NI NENGAH DARMIATI NI NYOMAN ARI MAYADEWI NI PUTU SWARI MEITAYANI NI WAYAN ARMINI NI WAYAN PUSPARINI DHARMAPUTRI NI WAYAN SUNITI Ningsih, Gusti Ketut Asti Dea Nona Au Waling Noorma Rosita Noorrizka V, Gusti Norine Nitya Ardiningrum Novayolla, Alsha Nurftatmelia Nurfadzilah, Puteri Gisyela NURUL KHOTIMAH NYOMAN AYU TRISNA ARI UTAMI OCTA FRANSISCA SITORUS Oktaviani, Faradila Putri Parwata, I Made Oka Pranata, Kadek Dwipayana Priyandani, Yuni Purwitasari, Neny PUTU PADMA MAHESWARI Qanita Afrannajla Rafid Khairi Santoso Rahayu, Titik Puji Rahmani, Risma Sekar RAMADHAN, MOCH BINTANG RIDA MELANI Rika Uyunul Mabilla RISMA IMROATUS SHOLIHAH Sabila, Bilqis Rif'atus Safitri, Zelda Santoso, Aina Cahyaning Sasadara, Maria Malida Vernandes Siahaan, Enjelina Silfa Fajriyatur Rosyidah Siti Rahmah Sri Lestari SRI LESTARI Sugiarto, Jennifer Angeline Sugijanto Sugijanto Sukaatmadja, I Suko Hardjono Sukorini, Anila Impian Sumarno . Suzana Syalsabila Aisyah Rahmawati Tarsat, Mosli Tho'ati, Fitri Nur Hidayatuth Timothy Alessandro Christaluary Lanapu Titania Fiska Ornelia Toetik Aryani Tri Widiandani TRISNA AGUNG PHABIOLA TRISNA AGUNG PHABIOLA Tristiana Erawati Tuan, Nguyen Thanh Tutiek Purwanti Umi Athiyah Umi Athiyah, Umi Ursulla Yulananda Anugraheni UTAMA, I WAYAN EKA KARYA Vanessa Emas Permata Sari VANI SILVANA Vaturrachma, Amelia Safira Putri VLORA VERONICA SIPANGKAR Wahyu Utami Wahyu Utami Wahyu Utami, Wahyu Walidah, Ziyana Wardah Zuhan Nafikhah WAYAN ADIARTAYASA WIDHIANTHINI WIDHIANTHINI Widiarsana Putra, I Made Widji Soeratri Wigunanda, I Wayan Surya Aditya Wiraatmaja, Wayan Wiwied Ekasari Yogi, Ida Bagus Putu Prajna YUDHA, I KADEK WISMA Yuni Priyandani Yuni Priyandani Yunita Nita Yusuf, Helmy Zhao, Tiejun Zulkamain, Bambang Subakti Zulkarnain, Bambang Subakti