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The Use of Lactic Acid Bacteria as Ruminant Probiotic Candidates Based on In Vitro Rumen Fermentation Characteristics Roni Ridwan; Win Ariga Bungsu; Wulansih Dwi Astuti; Rohmatussolihat Rohmatussolihat; Nurul Fitri Sari; Rusli Fidriyanto; Anuraga Jayanegara; Indah Wijayanti; Yantyati Widyastuti
Buletin Peternakan Vol 42, No 1 (2018): BULETIN PETERNAKAN VOL. 42 (1) FEBRUARY 2018
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v42i1.23317

Abstract

This research was conducted to select, to identify LAB isolates and to investigate the effects of the LAB as probiotics candidate in the rumen fermentation. Nine isolates exhibited the potency as candidate probiotics for cattle. The experiment was arranged in randomized block design with ten treatments and three different times of in vitro as a block. The substrate consisted of 70% forage and 30% concentrate proportion. The substrate was incubated at 39oC using serum bottle of 100 ml capacity for fermentation. Approximately 0.75 g of substrates was put inside the serum bottle glass and filled with 73 ml of buffered rumen fluid and 2 ml of LAB inoculant. Gas production was measured every 2, 4, 6, 8, 10, 12, 24, 48 and 72 h of the incubation period. Gas production kinetic was estimated by the Ørskov’s equation. The LAB with the highest gas production, as probiotics candidate, were identified using partial 16S rDNA sequence. The results of this research indicated that nine LAB produced high gas production in the range of 193-198 ml compare to that of control (173 ml). The addition of LAB in rumen fermentation resulted in digestibility 65-75%, organic matter digestibility 51-73%, and 6.67-6.68 pH. Based on the molecular identification, 8 isolates are Lactobacillus plantarum and 1 of uncultured bacteria. The LAB strain 32 L. plantarum showed the best for a ruminant probiotic candidate based on the in vitro rumen fermentation characteristic.
AMMONIATION OF RICE STRAW AND SUPPLEMENTATION OF Paraserianthes falcataria AND Sapindus rarak ON IN VITRO RUMEN FERMENTATION AND METHANE PRODUCTION Anuraga Jayanegara; Nanang Krisnawan; Yeni Widyawati; Asep Sudarman
Buletin Peternakan Vol 41, No 4 (2017): BULETIN PETERNAKAN VOL. 41 (4) NOVEMBER 2017
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v41i4.25549

Abstract

This study aimed to observe the effects of rice straw ammoniation and supplementation of Paraserianthes falcataria and Sapindus rarak on rumen fermentation and methane production in vitro. Rice straw was ammoniated by adding 2% urea. Rice straw, ammoniated rice straw, P. falcataria leaves and S. rarak fruits were oven-dried and finely ground. Experimental treatments were arranged as follow: rice straw (T1), ammoniated rice straw (T2), T2 80% + P. falcataria 20% (T3), T2 60% + P. falcataria 40% (T4), T2 80% + P. falcataria 10% + S. rarak 10% (T5), and T2 60% + P. falcataria 20% + S. rarak 20% (T6). An amount of 1 g sample from each treatment was added with 100 ml rumen fluid and buffer mixture (1:2 v/v), and incubated in a water bath at 39 ºC for 48 h. The incubation was performed in four replicates and each replicate was represented by four incubation bottles. Results showed that urea treatment increased gas production of rice straw at 24 and 48 h, higher ammonia production, higher IVDMD, and lower methane production as compared to the untreated rice straw (P<0.05). Addition of P. falcataria or S. rarak at lower level produced similar ammonia concentration as ammoniated rice straw whereas their addition at higher level decreased ammonia concentration (P<0.05). Paraserianthes falcataria addition to ammoniated rice straw decreased protozoa population (P<0.05) and S. rarak further decreased the fauna population (P<0.05) as well. Inclusion of S. rarak at 20% DM (T6) produced the lowest methane production both at 24 and 48 h after incubation and lowest methane production per unit of DM degraded. It was concluded that ammoniation of rice straw improved its nutritional quality with lower methane production, and its mixture with P. falcataria and S. rarak further increased the quality and lower the production of methane, respectively.        
Influence of Hemicell® Addition on Diets Containing Different Levels of Crude Fiber on Performance of Laying Hens Muhammad Ridla; Annisa Imran; Anuraga Jayanegara; Widya Hermana; Allaily Tarman
Buletin Peternakan Vol 43, No 3 (2019): BULETIN PETERNAKAN VOL. 43 (3) AUGUST 2019
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v43i3.44618

Abstract

This study was conducted to examine the effect of Hemicell® addition on diets containing different levels of crude fiber on performance and egg quality of laying hens. A total of 72 laying hens aged 21 weeks were randomly divided into 18 experimental units. A completely randomized design with factorial design 2×3 and 3 replications was employed in this study. Factor A was crude fiber level (5% and 8%) and factor B was the level of Hemicell®(0, 100x103 and 200x103 IU/kg ration).  Results showed that crude fiber significantly (P<0.01) increased feed consumption and decreased the performance of laying hens. The addition of Hemicell®in diets significantly (P<0.01) decreased feed consumption and increased the performance of laying hens. Hemicell®addition (100 IU) to a diet containing 8% crude fiber significantly (P<0.05) affected egg mass, feed conversion ratio, egg yolk score and egg weight. It can be concluded that diet with 8% crude fiber added with Hemicell® (100 IU) could increase egg mass, feed conversion ratio, egg weight and yolk color score.
EVALUASI RANSUM MENGANDUNG Indigofera zollingeriana TERHADAP ANAK KAMBING LEPAS SAPIH Suharlina Suharlina; D.A. Astuti; Nahrowi Nahrowi; A. Jayanegara; L. Abdullah
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 7 No 2 (2018): Pastura Vol. 7 No. 2 Tahun 2018
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1022.362 KB) | DOI: 10.24843/Pastura.2018.v07.i02.p02

Abstract

This study was objected to evaluate the nutritional values of ration containing Indigofera zollingeriana to post-weaning goat kids performans. Randomized completely design were used to four types of rations. The 16 heads of ettawah gradebreed post-weaning goad kids (14-21 body weight) were maintained during 8 weeks. I. zollingeriana forage were formulated into R1, R2, R3, and R4 concentrate feeds with proportion 0, 20, 40, and 60% I. zollingeriana, respectively. The rations were I0 (75% R1 + 25% napier grass), I20 (75% R2 + 25% napier grass), I40 (75% R3 + 25% napier grass), I60 (75% R4 + 25% napier grass), respectively. The variables observed were nutrient intake, digestibility, nitrogen utilization, blood nutrients, average daily gain (ADG) and feed efficiency. The data were analyzed using analysis of variance. The result showed that the crude protein (CP) intake of I40 was higher (P<0.05) than I0. The organic matter digestibility value of I20 was higher (P<0.05) than I60. The CP digestibility values of I20 and I40 were higher (P<0.05) than I0 and I60. The total digestible nutrient value of I0 and I20 were higher (P<0.05) than I40 and I60. The nitrogen (N) intake of I0 was less than (P<0.05) I40. The N retention of I40 was higher (P<0.05) than I0 and I60, while I20 was higher (P<0.05) than I0. The net nitrogen utilization of I20 and I40 were higher than I0 and I60. There were no significantly differences on total protein, albumin and immunoglobulin G values of blood serum. The cholesterol serum values of I40 and I60 were less (P<0.05) than I0 and I20. The ADG of I20 was higher (P<0.05) than I0 and I60. The feed efficiency of I20 was highest (P<0.05) than other rations. Key words: dairy goat, Indigofera zollingeriana, nitrogen utilization
KARAKTERISTIK FERMENTASI RUMEN TERHADAP BEBERAPA JENIS TANAMAN LEGUMINOSA Suharlina Suharlina; L. Abdullah; DA Astuti; Nahrowi Nahrowi; A Jayanegara
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 5 No 2 (2016): Pastura Vol. 5 No. 2 Tahun 2016
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.358 KB) | DOI: 10.24843/Pastura.2016.v05.i02.p02

Abstract

Penelitian ini dilaksanakan untuk mengevaluasi karakteristik fermentasi beberapa jenis leguminosa di dalam cairan rumen kambing secara in vitro. Desain penelitian menggunakan rancangan acak kelompok pada 7 jenis tanaman dalam 4 kelompok cairan rumen yang berbeda. Tanaman leguminosa yang digunakan antara lain, Alfalfa (Medicago sativa), Angsana (Pterocarpus indicus), Gamal (Gliricidia sepium), Indigofera (Indigofera zollingeriana), Kaliandra (Calliandra callothyrsus), Lamtoro (Leucaena leucochephala), dan turi (Sesbania grandiflora). Peubah yang diamati antara lain produksi gas total, kecernaan bahan kering, bahan organik, dan protein. Data dianalisis menggunakan analisis ragam (ANOVA). Hasil penelitian menunjukkan bahwa produksi gas tanaman indigofera dan alfalfa lebih memperlihatkan tren yang lebih tinggi dibandingkan empat tanaman lainnya. Kecernaan bahan kering (KCBK) dan bahan organik (KCBO) memperlihatkan perbedaan yang nyata (P<0,01) dimulai dari yang tertinggi adalah Indigofera, alfalfa, gamal, turi, lamtoro, kaliandra dan angsana. Terdapat interaksi yang nyata (P<0,05) antara perlakuan dan kelompok. Tanaman I zollingeriana memiliki karakteristik fermentasi rumen yang lebih baik dibandingkan tanaman leguminosa lainnya.Kata kunci: fermentasi rumen, in vitro, leguminosa
Analisis dan Komparasi Luaran Publikasi Ilmiah Sejumlah Perguruan Tinggi Muhammadiyah Milana Abdillah Subarkah; Anuraga Jayanegara
Jurnal Pendidikan Islam Vol. 12 No. 2 (2021)
Publisher : Universitas Muhammadiyah Prof Dr Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jpi.v12i2.7302

Abstract

This study aimed to analyze and to compare the output of scientific publications, both national and international, from a number of Muhammadiyah universities. The source of data used in this study was originated from the Science and Technology Index (SINTA) portal, and as many as 35 Muhammadiyah universities were the objects of this study. Data were analyzed using a correlation analysis and a multivariate analysis, namely principal component analysis (PCA). Results showed that the variables of Scopusindexed publication, SINTA-indexed publication, SINTA score, number of academic staffs, and the ratio between Scopus-indexed publication to the number of academic staffs had positive and significant correlations (P<0.001). The PCA results indicated that several universities, namely UM Surakarta, Ahmad Dahlan University and UM Yogyakarta were in a separate cluster from other Muhammadiyah universities, i.e., the superior cluster in terms of publication output. Other universities that close to themwere UM Malang and UHAMKA.
Increasing the Quality of Agricultural and Plantation Residues using Combination of Fiber Cracking Technology and Urea for Ruminant Feeds S. P. Dewi; M. Ridla; E. B. Laconi; A. Jayanegara
Tropical Animal Science Journal Vol. 41 No. 2 (2018): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1295.764 KB) | DOI: 10.5398/tasj.2018.41.2.137

Abstract

This experiment aimed to evaluate the decrease of the fiber fraction of some agricultural and plantation residues after being treated with Fiber Cracking Technology (FCT) and urea. The residues included rice straw, oil palm frond (OPF), oil palm empty fruit bunch (OPEFB), cocoa pod and coffee husk. They were added with 5% urea and incubated in FCT at temperature 135oC and pressure 2.3 atm for 2.5 h. The experimental treatments were arranged as a factorial design 5 × 2, in which the first factor was various agricultural and plantation residues (rice straw, OPF, OPEFB, cocoa pod and coffee husk) and the second factor was FCT application (untreated and treated with FCT + 5% urea), performed in 4 replicates. All treatments were subjected to Van Soest analysis and in vitro digestibility test. The decrease of fiber fraction was confirmed with Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR) methods. Results showed that FCT + 5% urea treatment decreased NDF, ADF, cellulose and lignin contents of all samples (P<0.05), and increased IVDMD and IVOMD in comparison to untreated samples (P<0.05). However, the treatment did not alter in vitro methane gas production and VFA profiles of the samples. Analyses using SEM, XRD and FTIR revealed that FCT + 5% urea treatment demolished cell wall component, decreased crystallinity index and cleaved fiber bonds. It was concluded that combination between FCT and urea 5% effectively enhances the quality of some fibrous feed materials.
Effects of Phloroglucinol on In Vitro Methanogenesis, Rumen Fermentation, and Microbial Population Density K. A. Sarwono; M. Kondo; T. Ban-Tokuda; A. Jayanegara; H. Matsui
Tropical Animal Science Journal Vol. 42 No. 2 (2019): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2019.42.2.121

Abstract

This study investigated the effect of phloroglucinol (1,3,5-trihydroxybenzene) supplementation alone on methane production, rumen fermentation profiles, and microbial population structure of mixed in vitro cultures. Treatments included a control group containing a substrate with no supplement, and substrates supplemented with 2, 4, 6, 8, or 10 mmol/L of phloroglucinol. The results revealed that phloroglucinol was able to decrease methane production in a dose-dependent manner. The highest decrease was observed with 8 and 10 mmol/L supplementations. The relative quantity of methanogen was not affected by phloroglucinol, whereas genus Coprococcus was increased with increasing concentrations of phloroglucinol (p<0.05). Total gas production, dry matter digestibility (DMD), and NH3-N were significantly lowered by phloroglucinol (p<0.001). Total short-chain fatty acid (SCFA) concentration was not affected by phloroglucinol. Acetate proportion increased with the addition of phloroglucinol at the expense of propionate (p<0.001). This might indicate the redirection of [H] from methane to acetate, and might be related to methane inhibition.. Our study concluded that supplementation of phloroglucinol alone could decrease methane production by inhibiting nutrient digestibility in the rumen and by possible redirection of rumen fermentation to acetate production. Genus Coprococcus could be an important actor for phloroglucinol metabolism in the rumen.
Performance, Physiological Status, and Rumen Fermentation Profiles of Pre- and Post-Weaning Goat Kids Fed Cricket Meal as a Protein Source D. A. Astuti; A. Anggraeny; L. Khotijah; S. Suharti; A. Jayanegara
Tropical Animal Science Journal Vol. 42 No. 2 (2019): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2019.42.2.145

Abstract

This study was conducted in two experiments with the objectives: (1) to evaluate nutrient intake, physiological status, and performance of pre-weaning goat kids fed with milk replacer containing cricket meal, and (2) to evaluate nutrient intake, rumen fermentation characteristics, blood profiles, and production performance of post-weaning etawah crossbred goats fed with cricket meal as a substitution of soybean meal. Twelve pre-weaning goat kids (3.78±0.50 kg BW) were divided into 3 treatments (4 replicates per treatment), i.e., kids fed with goat milk (GM), cow milk (CM), and milk replacer containing cricket meal (MR). In the second experiment, 12 post-weaning goats (12-14 kg BW) were fed with 3 dietary treatments, i.e., control ration (CM-0), ration containing 15% cricket meal (CM-15), and ration containing 30% cricket meal (CM-30). Parameters measured during 2 months observations were nutrient intake, physiological status, fermentation profiles, blood profiles, and performance. Both experiments used a completely randomized design. Results showed that there were no significant differences in physiological status parameters in pre-weaning goat kids fed with GM, CM or MR. The performance of kids fed with GM was significantly higher (120 g/d) than those fed with CM and MR treatments (p<0.05). In the second experiment, results showed that fat intake in CM-30 was higher than the other treatments (p<0.05), meanwhile dry matter and nutrient intakes were similar. The average daily gain (ADG), feed efficiency, and rumen fermentation profiles were similar in all treatments. It is concluded that cricket meal can be used in milk replacer without any negative effect on health status. CM-30 ration has good palatability without any negative effect on the rumen fermentation profiles.
The Assays of Bacteria-Yeast Consortia as Probiotics Candidates and Their Influences on Nutrients Utilization of Quails Diet A. Sofyan; R. S. H. Martin; E. B. Laconi; A. Jayanegara; H. Julendra; E. Damayanti; A. E. Suryani
Tropical Animal Science Journal Vol. 42 No. 3 (2019): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2019.42.3.196

Abstract

This study aimed to assay L. plantarum (P1), S. cerevisiae (P2), and its combination (P3) as probiotics candidates and their effects on nutrient digestibility in Japanese quails (Cortunix cortunix japonica) diets. In vitro assays were employed to evaluate the antibacterial activities against pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella pullorum), sensitivity to antibiotics (i.e., streptomycin, penicillin, and erythromycin), stress on bile salt and acidity condition. Evaluation of probiotics on in vivo nutrient utilization was carried out by administration of probiotics to laying quails in drinking water with various treatments (R0, control; R1, L. plantarum; R2, S. cerevisiae; R3, combination of R1+R2; R4, commercial feed additive). Results showed that the highest inhibitory activity was performed by P2 on S. aureus (17.28 mm). Treatments P2 and P3 were resistant to all the tested antibiotics, while P1 was resistant to streptomycin and penicillin. P1 was tolerant to bile salts whereas P2 was tolerant to gastric acidity conditions. For the in vivo experiment, quails fed R1, R2, and R4 had higher nitrogen retention as compared to R0 (p<0.05). However, all treatments revealed similar metabolizable energy values. The cluster analysis showed that quails consuming the combination of L. plantarum and S. cerevisiae were within the same cluster with the control but different clusters from the individual probiotics across all parameters, indicating a possible antagonistic effect between the two species.  In conclusion, the inclusion of L. plantarum or S. cerevisiae inhibits pathogenic bacteria without influencing nutrient utilization of quail diet.
Co-Authors A S Tjakradidjaja A Sofyan A. A. Affan A. A. Samsudin A. A. Samsudin A. Anggraeny A. Atabany, A. A. E. Suryani A. Fitri A. GUNAWAN A. Ikhwanti A. Irawan A. Salleh A. Sofyan A. Sofyan A. Sofyan A. Sudarman A. T. Wahyudi A.K. Takyi A.O. Salih Abdulmana, Sahidan Achmadi, S. S. Aditya, S. Adli, D. N. Adriana, Savira Astri Afandi, Frendy Ahmad Ahmad Sofyan Ahmad Sofyan Ahmad Sofyan Allaily Tarman Almira Firna Fitrianti Ludfi Alya Nur Zahra Amin, M. F. Amirul, F. M. A. Andrian, Dedek Anjas Asmara Samsudin Annisa Imran Ardani, V. Arief Sabdo Yuwono Arief, I. I. Asep Sudarman Awistaros Angger Sakti Ayinda, R. S. K. Ayu Septi Anggraeni Aziz Kustiyo Bambang Pramudya Noorachmat, Bambang Pramudya Basri Jumin, Hasan Budi Tangendjaja C. Hidayat C. SUMANTRI Cecep Hidayat Chung, T K D M Sinaga D. A. Astuti D. A. Astuti D. A. Astuti D. Setiawan D.A. Astuti DA Astuti DADIK PANTAYA Daulai, M. S. Dayat Dayat, Dayat Dede Rosyada Dedy Supriadin Desrial DEWI APRI ASTUTI Dias Indrasti Didid Diapari Diky Ramdani Dwinarto, Bondan E B Laconi E B Laconi E Palupi E Wina E. B. Laconi E. B. Laconi E. B. Laconi E. B. Laconi, E. B. E. Damayanti E. Wina E. Wina E.B. Laconi E.M. Hoffmann Effendi, Leo Adhar Elfawati Endang Warsiki Endrawati, Yuni Cahya Eneh Maesaroh Erica B. Laconi Erika B. Laconi Eylen Desy Novita Fahmi, Melta Rini FARAH FAHMA Fauzan, RM Muhammad Nur Febriantoko, Ardiansah Febrina, D. Fhonna, Fenda Alvionita Fitri Ramadhani Fitri, Ainissya Gumilang Khairulli H. A. Hassim H. A. Hassim, H. A. H. Herdian H. Julendra H. Matsui H. P S Makkar H.P S Makkar H.P.S. Makkar H.P.S. Makkar Hanny Hafiar Harahap, R. S. Hari Agung Adrianto Harningsih, Intan Sri Hasanah, Q. N. Hazen, K. Hendra Herdian Hendra Herdian Heri Ahmad Sukria Heru Nugraha Hindun Syarifah, Hindun I G Permana I Komang Gede Wiryawan I. G. Permana I. Ikhsan Ikhwan Shodiq Syifaudin ILHAM Iman Hernaman Indah Wijayanti Irawan Sugoro Irawan, A. Ismail Marzuki Ismail Marzuki Isnaini Dafri J. Nomura Johan, Arsyelina Husni Juniati, Indira K Becker K. A. Sarwono K. Becker K. Becker K. G. Wiryawan K. Nur, K. Khasrad . Krisnan, Rantan Kusuma, R. I. L. Abdullah L. Abdullah L. Abdullah L. Khotijah Laconi, Erika Budiarti Lendrawati Lendrawati Lendrawati, L. Leo, Jason Filbert Listyarini, K. M. Abdelbagi M. Adhyatma M. Kondo M. M. Sholikin M. Makmur M. Zamri-Saad Mafrika Ria Gita Solaya Mahmudah, Nurhayatun Martin, Rima Shidqiyya Hidayati Mauluddin, Dadan Mazlan, N. A. Melissa Melissa Milana Abdillah Subarkah Mira Andriani Mohamad Yamin Muhammad Alam Firmansyah Muhammad Arpah Muhammad Ridla Muis, Nurmaya Rahmawati Mukhtarom, Asrori Mulianda, R. Mutiara Rizky Raisa N. Laylli Nahri Idris Nahrowi Nahrowi Nahrowi Nanang Krisnawan Nanang Krisnawan Neni Sintawardani Nisrina, Aufa Novandri, B. Novia Qomariyah Nugraha Edhi Suyatma Nur Rochmah Kumalasari Nurfitriani, R. A. Nurrul Suri Aulia Nurul Fauziah Nurul Fitri Sari P. Yuliana Permana, I G Prameswara, Jordi Aditiya Prameswari, Febrina Pratama, Said Mirza Pristian Yuliana Pudji Muljono Qomariah, N. Qomariyah, Novia Qomariyah, Novia Qurrota A’yuni R A Nurfitriani R Ridwan R. Hana Nurfitrian Adjie R. K. Rusli R. P. Harahap R. P. Harahap R. Priyanto R. Ridwan R. S. H. Martin R. Solfaine Rahmatillah, Ririn Siti Raihan Raihan, Raihan Rakhmad Perkasa Harahap Rantan Krisnan Rardniyom, Chananpat Retnani, Y Retno Budi Lestari Retno Sri Hartati Mulyandari, Retno Sri Hartati Rima Shidqiyya Hidayati Martin Rini Purnawati Risyahadi, S. T. Rita Mutia Rizki Amalia Nurfitriani Rochimawati, Nur Riana Rohmah, Aenyfatchu Rohmatussolihat Rohmatussolihat Romantica, Krishna Prafidya Roni Ridwan Roni Ridwan Roni Ridwan Rudi Priyanto Rusli Fidriyanto Rusli, N. D. S. P. Dewi S. P. Dewi S. Suharti S. Suharti Saad, M. Z. Sabila, Maureen Sadarman Sadarman , Sadarman Sadarman Sadarman, S. Saeful Ansor Samadi Samadi Samadi Samadi Samsudin, A. A. Samsudin, Anjas Asmara Santi Ratnakomala Selly Anastassia Amellia Kharis Sholeha, Novia Amalia Sholikin, M. M. Silitonga, Rhoito Frista Sitti Wajizah Sitti Wajizah Suci Wulandari Sugiyono Sugiyono Suharlina Suharlina Suharti, Sri Sukria, H. A. Sumiati SUMINAR SETIATI ACHMADI Suryanegara, Lisman Syamsiyah, Dzorifatus T Sutardi T. Ban-Tokuda T. M. Syahniar T. Obitsu T. Sabhan T. Toharmat Tandio, Tjondroargo Tangendjaja, Budi Teuku Mujaddid Syahri Ar-Ridha Thamrin, Elfa Susanti Theo Mahiseta Syahniar Titi Candra Sunarti Toto Haryanto Tri Aminingsih Tri Rachmanto Prihambodo Tri Wijayanti Septiarini Tuti Suryati Usman Ali Utomo, D. W. Manalu W. W. Wardani Wardani, W. W. Wasmen Manalu Widjaja, H. Widya Hermana Wijayanti, I. Win Ariga Bungsu Wina, E Wulansih Dwi Astuti Y Retnani Y. Marlida Y. Retnani Y. Retnani Y. Yogianto, Y. Yantina, N. Yantyati Widyastuti Yantyati Widyastuti Yeni Widyawati Yeni Widyawati Yesi Chwenta Sari Yuli Retnani Yulianri Rizki Yanza Yuliansyah, Andika Boy Zain, M. Zulkarnain Zulkarnain Zulkifli, Sunarto