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Sugar, Acid Soluble Polysaccharide, and Total Phenolic Contents in Tropical Legumes and Their Relationships with In Vitro Nutrient Fermentability A. Ikhwanti; A. Jayanegara; I. G. Permana; W. W. Wardani; Y. Retnani; A. A. Samsudin
Tropical Animal Science Journal Vol. 43 No. 4 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2020.43.4.331

Abstract

Tropical legume is a type of C4 plant that has been adaptive to hot environments. Therefore, tropical legumes require energy reserves in the form of sugar and starch. This study aimed to explain the relationship between sugar, starch, and tannin contents of tropical legumes and their in vitro fermentation profiles. Samples of Bauhinia purpurea, Pterocarpus indicus, Tamarindus indica, Calopogonium mucunoides, Macroptilium atropurpureum, and Stylosanthes guianensis were tested by proximate analysis, Van Soest, and in vitro nutrient fermentability. The in vitro fermentability consisted of dry matter degradability (DMD), organic matter degradability (OMD), volatile fatty acid (VFA), methane and ammonia concentrations, and gas production. The gas production kinetics were determined as gas production from soluble nutrient (a), maximum gas production (a+b), and rate of gas production (c). All samples were tested using the orthogonal contrast test to compare in vitro fermentability characteristics between the different types of legumes and their tannin contents. Results revealed that the average content of the legumes dry matter examined was 22% with CP content of 15% -28%. The legumes in this study had average content of 3.74% sugar, 21.86% ASP, and 0.3% total phenolics (dry matter basis). Acid soluble polysaccharides content in legumes had a positive correlation with degradability, ammonia concentration, and total gas production (p<0.05). However, sugar content did not have a correlation with the other in vitro fermentation characteristics. The exploration of sugar and acid soluble polysaccharides content in tropical legumes might be profitable as these nutrients could increase feeding efficiency. Sugar did not have a significant relationship to the characteristics of fermentation, on the contrary, acid soluble polysaccharides had a very close relationship to the characteristics of fermentation. The total phenolics had a close relationship with the production of VFA and ammonia. Tree legumes have better digestibility than shrub legumes.
Enteric Methane Emissions and Rumen Fermentation Profile Treated by Dietary Chitosan: A Meta-Analysis of In Vitro Experiments R. P. Harahap; D. Setiawan; Nahrowi Nahrowi; S. Suharti; T. Obitsu; A. Jayanegara
Tropical Animal Science Journal Vol. 43 No. 3 (2020): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2020.43.3.233

Abstract

Chitosan is a natural compound obtained from deacetylation of chitin, which is a biopolymer present in the exoskeleton of crustaceans such as crabs and shrimp. The present study aimed to perform a meta-analysis from published studies regarding the effects of chitosan on methane emission and rumen fermentation profile of in vitro batch culture experiments. A total of 41 studies from 12 articles were integrated into a database. Parameters included were gas production, methane emission, rumen fermentation characteristics, microbial population, nutrient digestibility, and fatty acid profile. Data were analyzed according to mixed model methodology in which different studies were treated as random effects and chitosan addition levels were treated as fixed effects. Results showed that chitosan addition was able to reduce enteric methane emissions (p<0.001). Such methane decrease was accompanied by a decline in the protozoa population (p<0.05) and a tendency of methanogen reduction (p<0.1). The increasing chitosan level was associated with a decrease in total VFA and ammonia concentrations (both at p<0.001). Chitosan addition decreased acetate proportion (p<0.001) while elevated propionate proportion (p<0.001). Chitosan was associated with an increase of dry matter digestibility, crude protein digestibility, and neutral detergent fiber digestibility (p<0.001). Chitosan increased concentrations of C18:3n3 (p<0.05), conjugated linoleic acid (p<0.01) and polyunsaturated fatty acids (p<0.01) while decreased concentration of saturated fatty acids (p<0.001). It can be concluded that chitosan addition can mitigate enteric methane emission and alters rumen fermentation profiles in a favorable direction.
Supplementation of Dietary Nano Zn-Phytogenic on Performance, Antioxidant Activity, and Population of Intestinal Pathogenic Bacteria in Broiler Chickens C. Hidayat; Sumiati Sumiati; A. Jayanegara; E. Wina
Tropical Animal Science Journal Vol. 44 No. 1 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.1.90

Abstract

Zinc is one of the essential minerals that are important for poultry. The disadvantage of Zn in the conventional form is its low bioavailability. One of the efforts to increase the bioavailability of Zn is to make it in a nano form. Nano Zn-Phytogenic (NZP), is a combination of Zn and phytogenic compounds of plants in nanoparticle size. The NZP was self-produced utilizing the green synthesis process of inorganic Zn and guava leave extract (Psidium guajava). The objective of this study was to evaluate the effects of supplementation NZP in diet on the performance, antioxidant status, and population of pathogenic intestinal bacteria (Escherichia coli and Salmonella sp) of broilers chicken. This study used 180 males and 180 females of Lohman broilers day old chick (DOC). The experiment was subjected to a completely randomized design with 6 (six) treatments and 5 (five) replications, and each experimental unit consisted of 12 DOCs (6 males and 6 females). The treatment given in this study were; R1= basal diet; R2= R1 + Zn Sulfate (90 mg Zn/kg) + 5.32 mg/kg guava leaf flour with 12.82% water content (added as a source of phytogenic compounds); R3= R1 + NZP (45 mg Zn/kg); R4= R1 + NZP (90 mg Zn/kg); R5= R1 + NZP (135 mg Zn/kg); R6= R1 + NZP (180 mg Zn/kg). The variables observed were performance, antioxidant activity in meat, and population of pathogenic intestinal bacteria (E. coli and Salmonella sp) of broiler chicken. The results showed that the addition of NZP up to a dose of 90 mg Zn/kg in the diet improved (p<0.05) body weight gain compared to the basal diet. The addition of NZP had no significant effect on the FCR. The addition of NZP increased (p<0.05) SOD activity in meat when compared with the dietary treatment without NZP. Groups of chicken fed NZP (R3, R4, R5, R6) had significantly (p<0.05) lower E. coli and Salmonella sp population. It could be concluded that the addition of NZP up to a dose of 90 mg Zn/kg in the diet of broiler chicken had positive benefits in improving performance, increasing antioxidant activity, and reducing pathogenic intestinal bacteria (E. coli and Salmonella sp).
A Meta-analysis of Antimicrobial Peptide Effects on Intestinal Bacteria, Immune Response, and Antioxidant Activity of Broilers M. M. Sholikin; A. T. Wahyudi; A. Jayanegara; J. Nomura; Nahrowi Nahrowi
Tropical Animal Science Journal Vol. 44 No. 2 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.2.188

Abstract

This study used a meta-analysis to systematically assess the effect of antimicrobial peptide (AMP) addition on the number of bacteria, immune responses, and antioxidant activity of broilers. The database was compiled from 29 post evaluation articles that were found in search engines consisted of 36 experiments and 111 data. The mixed model method was used to assess the effect of AMP, with AMP addition level as a fixed effect and experiment as a random effect. The fixed effect was tested for linear and quadratic models. The quadratic model was retained when significant at p<0.05 but turned into its corresponding linear model when insignificant. In the starter phase, AMP addition decreased the number of bacteria in the ileum (coliform and total aerobic bacteria (TAB); (p<0.05), the caecum (Clostridium spp., Escherichia coli, coliform, and lactic acid bacteria (LAB); p<0.05), and excreta (Clostridium spp.; p<0.1). Similarly, the number of bacteria also declined in the ileum (Escherichia coli, p<0.05; TAB, p<0.1), the caecum (LAB; p<0.1), and excreta (Clostridium spp.; p<0.05) of broilers in the finisher phase. There were significant improvements in immune response and antioxidant activity in starter broiler, as indicated by the titer of Newcastle disease (ND) antibody, bursal index, spleen index, and thymus index (p<0.05) due to AMP addition. Variables of immunoglobulin M (IgM), cluster of differentiation 4 (CD4), ND antibody titer, bursal index, spleen index, and thymus index were also significantly increased (p<0.05) while superoxide dismutase activity (SOD activity) tended to increase (p<0.1) in finisher broiler following the AMP addition. In short, AMP addition is able to suppress the number of pathogenic bacteria and increase the immune response and antioxidant activity of broilers.
Clinical Signs and Blood Variables of Pregnancy Toxemia Goats during Late Gestation and Postpartum A. A. Affan; A. Salleh; M. Zamri-Saad; A. Jayanegara; H. A. Hassim
Tropical Animal Science Journal Vol. 45 No. 1 (2022): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2022.45.1.84

Abstract

Pregnancy toxemia is one of the common metabolic diseases in ruminant, which has caused a huge economic impact on the dairy industry. Thus, this study aimed to describe the clinical and blood changes in pregnant goats following induction of pregnancy toxemia. Twelve pregnant goats were divided into control (n=3) and treatment (n=9) groups. The control was fed a diet with adequate energy while the treatment group was exposed to 50% reduction in the energy intake to induce pregnancy toxemia. Blood samples were collected at weekly intervals for biochemical analyses, which included glucose, beta-hydroxybutyrate (BHBA), free fatty acid (FFA), calcium, sodium, potassium, chloride, cortisol, and insulin. On days 20 (PK20) and 40 (PK40) post-induction, 3 induced and 1 control goat was slaughtered while the remaining 3 induced goats were provided with a normal balanced diet to allow recovery (PKRD). The induction resulted in acute pregnancy toxemia after 20 days with clinical signs including weakness, loss of body condition, and recumbency. At 40 days, chronic pregnancy toxemia resulted in signs such as incoordination and abortion. There was significant (p<0.05) decrease in the glucose, insulin, calcium, and potassium levels in the induced goats while the concentrations of BHBA, FFA, and cortisol were significantly (p<0.05) higher. Furthermore, the blood profiling was significantly (p<0.05) different between the PK20, PK40, and PKRD groups and was strongly associated with the presence of clinical signs and ketone bodies in the urine. The 50% reduction in energy intake resulted in acute pregnancy toxemia after 20 days and chronic pregnancy toxemia after 40 days. In conclusion, serum biochemical profile is a potential biomarker to assess the mild and severe pregnancy toxemia in does during the late gestation and postpartum period through changes in blood profiling.
Propolis Supplementation on Broiler Chicken Performances, Nutrient Digestibility, and Carcass Characteristics: A Meta-Analysis Sadarman; A. Irawan; C. Hidayat; Elfawati; M. M. Sholikin; R. P. Harahap; R. K. Rusli; R. Solfaine; A. Sofyan; Nahrowi; A. Jayanegara
Tropical Animal Science Journal Vol. 44 No. 4 (2021): Tropical Animal Science Journal
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5398/tasj.2021.44.4.425

Abstract

This meta-analysis was conducted to investigate the effect of dietary propolis supplementation on broiler performance, carcass characteristics, and nutrient digestibility evaluated at the starter and finisher phases. An online literature search was conducted using scientific platforms of Science Direct, PubMed, and Google Scholar to identify the studies utilizing propolis as dietary supplementation in broiler chickens. A total of 39 publications comprising of 58 experiments met the inclusion criteria. A mixed model procedure for meta-analysis was performed, considering the studies as a random effect and levels of propolis as a fixed effect. Regression analysis suggested that average daily gain (ADG), body weight, and average daily feed intake (ADFI) at starter and finisher phases were quadratically affected by supplementing propolis in the diet (p<0.01). Propolis also lowered the feed conversion ratio (FCR) throughout the periods (p<0.05). In addition, a positive effect of propolis inclusion was also observed on breast meat percentage (p<0.05). However, there was a negative linear relationship between propolis and the digestibility of dry matter (DMD) and organic matter (OMD) (p<0.01) at the starter period. Conversely, there was a linear increase in DMD (p<0.05) and a tendency to improve apparent metabolizable energy (AME) and phosphorus digestibility at the finisher period (p<0.10). Overall, it can be concluded that dietary propolis supplementation promoted better growth performance and feed efficiency when given at ~1.66-2.13 g/kg diets for starter and finisher phases in the broiler chickens. The effect of propolis on nutrient digestibility was positive at the finisher phase while it was detrimental at the starter phase.
Teknologi Penyiapan Pakan Protein Moderate dan Strategi Penyiapannya untuk Meningkatkan Produktivitas Maggot : Preparation Technology of Moderate Protein Feed and Its Strategy to Increase Maggot Productivity Isnaini Dafri; Nahrowi; A Jayanegara
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 1 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.1.25-29

Abstract

The aim of this research was to prepare moderate protein diet and evaluate its quality to increase maggot productivity. This research was conducted from the egg stage to the pre-pupae stage. One gram of larval eggs was used for each replication. The substrates used were fruit waste, household waste, and concentrate diet. This study used a completely randomized design with 2 treatments and 6 replications. Treatments were P0 (control feed of fruit waste and household waste) and P1 (moderate protein treatment feed). Control feed and moderate protein treatment were given as much as 13 kg during maintenance. The variables measured were dry matter consumption (grams), maggot weight (grams), pre-pupae percentage, substrate reduction (%), FCR, bioconversion (%), and WRI (Waste Reduction Index). Data were analyzed using paired sample T test. The results showed that BSF larvae receiving moderate protein treatment were significantly higher (p<0.05) compared to control feed for substrate consumption parameters, substrate reduction, and WRI. The conclusion from this research is the quality of feed with moderate protein content of 18,30 % which was prepared using a crude protein and energy balance approach was better than feed based on papaya fruit waste and household waste seen from the aspect of increasing substrat reduction, improving performance, prepupa metamorphosis, and increasing bioconversion of BSF larvae. Key words: bioconversion, larvae BSF, maggot, performance, waste
Aktivitas Antioksidan Ekstrak Daun Sambiloto (Andrographis Paniculata) dan Ekstrak Kulit Manggis (Garcinia Mangostana) terhadap Aflatoksin B1 pada Jagung: Antioxidant Activity of Andrographis paniculata Leaves Extract and Garcinia mangostana Peel Extract to aflatoxin B1 in Corn Rima Shidqiyya Hidayati Martin; E B Laconi; A Jayanegara
Jurnal Ilmu Nutrisi dan Teknologi Pakan (Nutrition and Feed Technology Journal) Vol. 20 No. 1 (2022): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.20.1.30-37

Abstract

The objective of this study was to analyze the active compound of Garcinia mangostana (GME) and Andrographis paniculata (APE) extracts and the effectiveness of these extracts against aflatoxin B1 (AFB1) which had contaminated corn. The experimental design of this study was a completely randomized factorial design. Factor A was the addition of extract, A0=without addition, A1=addition of 0.08% GME, A2=addition of 0.16% GME, A3=addition of 0.08% APE, A4=addition of 0.16% APE. Factor B was the incubation period, B1=day 0, B2=day-2, B3=day-4. The research variables were extract yield, total phenolic content, antioxidant activity, corn moisture content and gross energy, AFB1 content, production and percentage of inhibition of AFB1. The data obtained were analyzed for variance and Duncan’s test. The results showed that GME and APE had yields of 16.68% and 5.49%, total phenolic content of 125.28 and 12.62 mg GAE g extract-1 and antioxidant activity of 29.82 and >200 ppm respectively. The addition of extract to each treatment during incubation significantly interacted with AFB1 production. However, the gross energy in each treatment was only affected by the incubation time, while the moisture content increased on the second day to 13.29%. It can be concluded that the addition of 0.16% GME could inhibit the production of AFB1 which was higher than the addition of other extracts during incubation. Key words: aflatoxin B1, Andrographis paniculata, corn, Garcinia mangostana
PHYSICAL AND CHEMICAL PROPERTIES OF ORGANIC MINERAL ADDITIVE FOR RUMINANT THROUGH BIOLOGICALLY INCORPORATED BY SACCHAROMYCES CEREVISIAE IN DIFERENCE SUBSTRATES Ahmad Sofyan; Hendra Herdian; Awistaros Angger Sakti; Gumilang Khairulli; Anuraga Jayanegara
Jurnal Kimia Terapan Indonesia Vol 17, No 2 (2015)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.832 KB) | DOI: 10.14203/jkti.v17i2.30

Abstract

This research was conducted to evaluate physical and chemical properties of organic mineral additive incorporated by Saccharomyces cerevisiae which was cultivated on different growth media consisted of cassava flour and rice bran, respectively. Treatment was arranged on completely randomized design consisted of rice bran without inoculants (RBo), rice bran with inoculants (RBi), cassava flour without inoculants (CFo), cassava flour with inoculants (CFi). Substrates were fortified by microminerals contained of Cu (100 ppm), Mn (100 ppm), Zn (100 ppm), I (10 ppm), Fe (2.5 ppm) and Co (2 ppm). Inoculation of S. cerevisiae could reduce fungal contamination. Physical characteristic of cassava flour was better in flavour and texture than rice bran in which tends to rancidity. Nutrients composition was similar in cassava and rice bran however crude fibre content in rice bran tends to be higher after fermentation. Mineral content (Zn and Fe) relatively decreased and in substrate supplemented by inoculant and rice bran had higher than cassava. In summary, micromineral was incorporated in cassava flour with inoculated S. cerevisiae had better than rice bran.Keywords: Organic mineral, cassava flour meal, S. cerevisiae, rice bran, ruminant.
Prediksi Kandungan Lignin pada Dedak Padi Bercampur Sekam Menggunakan Tekstur Statistik dan KNN Eylen Desy Novita; Aziz Kustiyo; Anuraga Jayanegara; Toto Haryanto; Hari Agung Adrianto
Jurnal Ilmu Komputer dan Agri-Informatika Vol 9 No 1 (2022)
Publisher : Departemen Ilmu Komputer, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jika.9.1.58-69

Abstract

Adulteration in rice bran happens quite high due to the expensive price of rice bran. Mixing the rice bran with husk could decrease the rice bran quality because the content of crude fiber and lignin cointained in husk are anti-nutrients. Lignin content can be estimated by the texture of rice bran mixed with husk image. This study aimed to analyze the texture of rice bran mixed with husk image using run length feature extraction method with k-nearest neighbour (KNN) classification. The images of rice bran mixed with husk were taken using Dino Capture digital microscope with magnification 200 times. The images were generated with the spatial resolution of 640×480 pixels in a bitmap format. Those images were converted from RGB into grayscale in preprocessing phase, then the result of grayscale images were enhanced using histogram equalization as image enhancement method. The training and testing was determined using 5-fold cross validation with 3 repetition. The result of KNN classification with 7 features showed the highest accuracy of 74.55%.
Co-Authors A S Tjakradidjaja A Sofyan A. A. Affan A. A. Samsudin A. A. Samsudin A. Anggraeny A. Atabany, A. A. E. Suryani A. Fitri A. GUNAWAN A. Ikhwanti A. Irawan A. Salleh A. Sofyan A. Sofyan A. Sofyan A. Sudarman A. T. Wahyudi A.K. Takyi A.O. Salih Abdulmana, Sahidan Achmadi, S. S. Aditya, S. Adli, D. N. Adriana, Savira Astri Afandi, Frendy Ahmad Ahmad Sofyan Ahmad Sofyan Ahmad Sofyan Allaily Tarman Almira Firna Fitrianti Ludfi Alya Nur Zahra Amin, M. F. Amirul, F. M. A. Andrian, Dedek Anjas Asmara Samsudin Annisa Imran Ardani, V. Arief Sabdo Yuwono Arief, I. I. Asep Sudarman Awistaros Angger Sakti Ayinda, R. S. K. Ayu Septi Anggraeni Aziz Kustiyo Bambang Pramudya Noorachmat, Bambang Pramudya Basri Jumin, Hasan Budi Tangendjaja C. Hidayat C. SUMANTRI Cecep Hidayat Chung, T K D M Sinaga D. A. Astuti D. A. Astuti D. A. Astuti D. Setiawan D.A. Astuti DA Astuti DADIK PANTAYA Daulai, M. S. Dayat Dayat, Dayat Dede Rosyada Dedy Supriadin Desrial DEWI APRI ASTUTI Dias Indrasti Didid Diapari Diky Ramdani Dwinarto, Bondan E B Laconi E B Laconi E Palupi E Wina E. B. Laconi E. B. Laconi E. B. Laconi E. B. Laconi, E. B. E. Damayanti E. Wina E. Wina E.B. Laconi E.M. Hoffmann Effendi, Leo Adhar Elfawati Endang Warsiki Endrawati, Yuni Cahya Eneh Maesaroh Erica B. Laconi Erika B. Laconi Eylen Desy Novita Fahmi, Melta Rini FARAH FAHMA Fauzan, RM Muhammad Nur Febriantoko, Ardiansah Febrina, D. Fhonna, Fenda Alvionita Fitri Ramadhani Fitri, Ainissya Gumilang Khairulli H. A. Hassim H. A. Hassim, H. A. H. Herdian H. Julendra H. Matsui H. P S Makkar H.P S Makkar H.P.S. Makkar H.P.S. Makkar Hanny Hafiar Harahap, R. S. Hari Agung Adrianto Harningsih, Intan Sri Hasanah, Q. N. Hazen, K. Hendra Herdian Hendra Herdian Heri Ahmad Sukria Heru Nugraha Hindun Syarifah, Hindun I G Permana I Komang Gede Wiryawan I. G. Permana I. Ikhsan Ikhwan Shodiq Syifaudin ILHAM Iman Hernaman Indah Wijayanti Irawan Sugoro Irawan, A. Ismail Marzuki Ismail Marzuki Isnaini Dafri J. Nomura Johan, Arsyelina Husni Juniati, Indira K Becker K. A. Sarwono K. Becker K. Becker K. G. Wiryawan K. Nur, K. Khasrad . Krisnan, Rantan Kusuma, R. I. L. Abdullah L. Abdullah L. Abdullah L. Khotijah Laconi, Erika Budiarti Lendrawati Lendrawati Lendrawati, L. Leo, Jason Filbert Listyarini, K. M. Abdelbagi M. Adhyatma M. Kondo M. M. Sholikin M. Makmur M. Zamri-Saad Mafrika Ria Gita Solaya Mahmudah, Nurhayatun Martin, Rima Shidqiyya Hidayati Mauluddin, Dadan Mazlan, N. A. Melissa Melissa Milana Abdillah Subarkah Mira Andriani Mohamad Yamin Muhammad Alam Firmansyah Muhammad Arpah Muhammad Ridla Muis, Nurmaya Rahmawati Mukhtarom, Asrori Mulianda, R. Mutiara Rizky Raisa N. Laylli Nahri Idris Nahrowi Nahrowi Nahrowi Nanang Krisnawan Nanang Krisnawan Neni Sintawardani Nisrina, Aufa Novandri, B. Novia Qomariyah Nugraha Edhi Suyatma Nur Rochmah Kumalasari Nurfitriani, R. A. Nurrul Suri Aulia Nurul Fauziah Nurul Fitri Sari P. Yuliana Permana, I G Prameswara, Jordi Aditiya Prameswari, Febrina Pratama, Said Mirza Pristian Yuliana Pudji Muljono Qomariah, N. Qomariyah, Novia Qomariyah, Novia Qurrota A’yuni R A Nurfitriani R Ridwan R. Hana Nurfitrian Adjie R. K. Rusli R. P. Harahap R. P. Harahap R. Priyanto R. Ridwan R. S. H. Martin R. Solfaine Rahmatillah, Ririn Siti Raihan Raihan, Raihan Rakhmad Perkasa Harahap Rantan Krisnan Rardniyom, Chananpat Retnani, Y Retno Budi Lestari Retno Sri Hartati Mulyandari, Retno Sri Hartati Rima Shidqiyya Hidayati Martin Rini Purnawati Risyahadi, S. T. Rita Mutia Rizki Amalia Nurfitriani Rochimawati, Nur Riana Rohmah, Aenyfatchu Rohmatussolihat Rohmatussolihat Romantica, Krishna Prafidya Roni Ridwan Roni Ridwan Roni Ridwan Rudi Priyanto Rusli Fidriyanto Rusli, N. D. S. P. Dewi S. P. Dewi S. Suharti S. Suharti Saad, M. Z. Sabila, Maureen Sadarman Sadarman , Sadarman Sadarman Sadarman, S. Saeful Ansor Samadi Samadi Samadi Samadi Samsudin, A. A. Samsudin, Anjas Asmara Santi Ratnakomala Selly Anastassia Amellia Kharis Sholeha, Novia Amalia Sholikin, M. M. Silitonga, Rhoito Frista Sitti Wajizah Sitti Wajizah Suci Wulandari Sugiyono Sugiyono Suharlina Suharlina Suharti, Sri Sukria, H. A. Sumiati SUMINAR SETIATI ACHMADI Suryanegara, Lisman Syamsiyah, Dzorifatus T Sutardi T. Ban-Tokuda T. M. Syahniar T. Obitsu T. Sabhan T. Toharmat Tandio, Tjondroargo Tangendjaja, Budi Teuku Mujaddid Syahri Ar-Ridha Thamrin, Elfa Susanti Theo Mahiseta Syahniar Titi Candra Sunarti Toto Haryanto Tri Aminingsih Tri Rachmanto Prihambodo Tri Wijayanti Septiarini Tuti Suryati Usman Ali Utomo, D. W. Manalu W. W. Wardani Wardani, W. W. Wasmen Manalu Widjaja, H. Widya Hermana Wijayanti, I. Win Ariga Bungsu Wina, E Wulansih Dwi Astuti Y Retnani Y. Marlida Y. Retnani Y. Retnani Y. Yogianto, Y. Yantina, N. Yantyati Widyastuti Yantyati Widyastuti Yeni Widyawati Yeni Widyawati Yesi Chwenta Sari Yuli Retnani Yulianri Rizki Yanza Yuliansyah, Andika Boy Zain, M. Zulkarnain Zulkarnain Zulkifli, Sunarto