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Pengaruh Penambahan Nutrient (NPK dan Pupuk Urea) terhadap Bioethanol Hasil Fermentasi Biji Alpukat (Persea americana Mill.) Putri, Zakyya Septiana; Ruyani, Aceng; Uliyandari, Mellyta; Wardana, Rendy Wikrama; Sukarso, A. A.
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11283

Abstract

Bioethanol is an energy source obtained through fermentation. In bioethanol production, Persea americana seeds are used as a fermentation medium because they contain lots of carbohydrates. This research aims to determine the effect of additional nutrients (NPK and urea fertilizer) required by Saccharomyces cerevisiae by varying the nutritional variables in the form of NPK and urea fertilizer. The process of making bioethanol goes through stages: making Persea americana seed flour, hydrolysis process using 6% HCl, then fermentation process, and distillation process. This research was carried out with the addition of Nutrients in the form of NPK and Urea Fertilizer with 5 treatments, namely: A) 100% yeast (no addition of Nutrients); B) 100% NPK (4 g); C) 100% Urea Fertilizer (4 g); D) 50% NPK and 50% Urea Fertilizer (4 g NPK and 4 g Urea Fertilizer); and E) 75% NPK and 25% Urea Fertilizer (3 g NPK and 1 g Urea Fertilizer), and varying the length of fermentation time to 13, 19, and 25 days. Success parameters are assessed through the bioethanol yield produced by each treatment. The research results showed that the highest bioethanol (29.7 ± 0.47%) was composition E (NPK 75% and Urea Fertilizer 25%) with a fermentation time of 25 days.
Giving a combination of snails (Achatina fulica) and coconut dregs on the growth and protein content of maggots (Hermetia illucens) Purnama, Intan; Ruyani, Aceng; Yani, Ariefa Primair; Johan, Henny; Parlindungan, Deni; Nursa’adah, Euis
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica, Vol. 12: No. 2 (August, 2025)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v12i2.22660

Abstract

This study aims to determine the effect of providing a combination of snails and coconut dregs as feed on the growth and protein content of Black soldier Fly (BSF) maggots. The method used is an experiment with a Completely Randomized Design (CRD) consisting of five treatments and three replications. The treatments consist of: P1 (100% snails), P2 (75% snails: 25% coconut dregs), P3 (50%: 50%), P4 (25%: 75%), and P5 (100% coconut dregs). Maggot maintenance was carried out for 14 days with feeding carried out every 2 days in the morning at 10:00. The research data observed included Weight, Thickness, Length which were analyzed statistically using the ANOVA test and the test was continued with the Least Significant Difference (LSD) test, and the Kruskal wallis test if the data was not normal, temperature and humidity, Feed consumption, Specific growth rate (SGR), Waste reduction index (WRI), and Protein content and water content were analyzed using the automatic kjeldahl test. The results of the study showed that the provision of snail and coconut dregs feed had a very significant effect on the growth of maggots (P <0.05) both in weight and thickness, but had no significant effect (P>0.05) on the growth of maggot length. Treatment P1 (100% snail) gave the best results with an average weight of 0.09 g, a length of 13.4 mm, and the highest protein content of 27.49%. Treatment P5 (100% coconut dregs) showed the lowest results in all main parameters. It was concluded that snail feed was more optimal in supporting the growth and protein content of BSF maggots. The high animal protein content and soft texture are the main reasons, which show the potential of snails as a highly nutritious alternative feed for BSF maggot cultivation. Keywords: BSF Maggots; Coconut dregs; Growth; Snails
Kuntze Metabolite Profile and Antioxidant Activity of Vincetoxicum villosum (Blume) Kuntze Sari, Anjeli Rama; Ruyani, Aceng; Ismaini, Lily; Hartuti, Endah Dwi
Chimica et Natura Acta Vol 13, No 3 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n3.60012

Abstract

Vincetoxicum villosum belongs to the Apocynaceae family. V. villosum is often used as a traditional medicine for treating jaundice, gallstones, kidney stones, hepatitis B, and liver diseases. This study aims to determine the metabolite profile and antioxidant activity of V. villosum to provide new information and serve as a reference for its safe use as a medicinal plant. The methods used to determine the metabolite profile involved GC-MS, and antioxidant activity was assessed using the DPPH method. GC-MS analysis showed that V. villosum leaf extract contains n-hexadecanoic acid, phytol, 9,12,15-octadecatrienoic acid (Z,Z,Z)-, and 2,4-di-tert-butylphenol which have potential antioxidant activity. The antioxidant activity in V. villosum leaves is classified as very weak, with  IC50 ranging 0.6 – 1.6 mg/mL, with the best antioxidant activity observed in polar solvents (ethanol extract) with a maceration time of 72 hours. The highest total phenol content was obtained using ethanol solvent with a maceration time of 72 hours, amounting to 416.17 mg GAE/g, and the highest total flavonoid content was obtained using ethyl acetate solvent with a maceration time of 24 hours, amounting to 168.78 mg QE/g
Study of dogania subplana aclimatization in the turtle learning center university of bengkulu Marliansa, Meiredo; Karyadi, Bhakti; Parlindungan, Deni; Ruyani, Aceng; Nursa'adah, Euis
JPBIO (Jurnal Pendidikan Biologi) Vol 10, No 2 (2025): November 2025
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v10i2.4953

Abstract

The forest softshell turtle (Dogania subplana) is a species increasingly threatened by habitat destruction and overexploitation. This study aims to identify the acclimatization process of D. subplana in an artificial environment, specifically, an aquarium at the Turtle Learning Center (TLC). The research was conducted over 30 days from July to August 2024. Observations were made on daily behavior, body temperature, and health condition, individual biometric measurements, and environmental (abiotic) parameters. The results showed that daily behaviors such as feeding, basking, and hiding occurred regularly, indicating the species' ability to adapt to a new environment. The turtles experienced an increase in body weight, while growth in carapace length and width was very slow. The body temperature of D. subplana varied in accordance with the surrounding environmental temperature, reflecting its dependence on ambient conditions. Some individuals experienced minor health issues, such as fungal infections, but the majority remained active and healthy. Environmental factors such as temperature, humidity, pH, dissolved oxygen, total dissolved solids, and light intensity were within ranges conducive to acclimatization. These findings suggest that the aquarium at the TLC is quite effective as an ex situ conservation facility in supporting the adaptation process of D. subplana  outside its natural habitat.
Effect honje forest fruit juice administration on hemoglobin levels following mercury chloride treatment in mice Iskandar, Aiza Sabina Putri; Ruyani, Aceng; Parlindungan, Deni; Johan, Henny; Mayub, Afrizal
JPBIO (Jurnal Pendidikan Biologi) Vol 10, No 2 (2025): November 2025
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v10i2.5561

Abstract

Exposure to mercury chloride (HgCl?) is known to induce oxidative stress, which negatively affects the hematological system, including a decrease in hemoglobin levels. This study aims to analyze the effect of honje fruit juice (Etlingera hemisphaerica), known for its high antioxidant content, on hemoglobin levels in mice (Mus musculus) exposed to mercury chloride. This study used an experimental method involving several groups of mice, namely group K1 (control), group K2 (given honje fruit juice), and group K3 (injected with mercury and given honje fruit juice). Hemoglobin levels were measured using a Sahli tube. The results showed that honje fruit juice significantly increased hemoglobin levels in mice exposed to mercury chloride compared to the control group. These findings indicate that the antioxidant content in honje fruit can help reduce the toxic effects of mercury chloride on the circulatory system. Therefore, honje fruit juice has the potential to be developed as a natural therapeutic agent in efforts to mitigate the toxic effects of mercury chloride.
Development of a Manual Book on Processing Flour of Mole Crab to Provide Students Science Process Skills Widia Gusti; Aceng Ruyani; Urip Santoso; Bhakti Karyadi; Euis Nursaadah
IJIS Edu : Indonesian Journal of Integrated Science Education Vol 7, No 2 (2025): July 2025
Publisher : UIN Fatmawati Sukarno Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29300/ijisedu.v7i2.6012

Abstract

This study is an experimental model a group pretest-posttest design. This study aims to: 1) Describe the feasibility of the manual book in learning to train students science process skills based on the results of flour processing through validity tests and trials, 2) Describe the results of improving students science process skills based on the manual book of flour processing made from mole crab. Validation of the manual book was carried out by experts to determine the feasibility of the content, language and design aspects, the results is Aiken-V score of 0.86 (valid). The implementation of the use of manual books in learning was carried out on students majoring in culinary arts and industry at SMKN 7 Bengkulu. Students’ science process skills (SPS) (Observation, Interpretation, Hypothesize, Design an Experiment, Implementing Concept and Using tools and Materials) were measure Based on the results of the pre-test and post-test, Students majoring in Industry has n-gain score 0.41 (moderate) while the students majoring in culinary has n-gain score 0.70 (moderate). The use of manual book in learning is suitable for use as a teaching material for students because it can stimulate and improve students’ science process skills.
Local Potential of Kabau (Archidendron bubalinum (Jack) I.C Nielsen): A Comprehensive Literature of Traditional Uses, Phytochemistry, Pharmacology and Learning Resources Potential Erik Perdana Putra; Aceng Ruyani; Fitri April Yanti
IJIS Edu : Indonesian Journal of Integrated Science Education Vol 6, No 2 (2024): July 2024
Publisher : UIN Fatmawati Sukarno Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29300/ijisedu.v6i2.10075

Abstract

Kabau (Archidendron bubalinum (Jack) I.C. Nielsen) is a lesser-known legume native to Sumatra and surrounding regions that is widely used as food and traditional medicine, but its scientific and educational potential remains underexplored. This study presents a qualitative–descriptive literature review synthesizing ethnobotanical, phytochemical, pharmacological, and educational evidence on kabau, based on peer-reviewed national and international publications indexed in databases such as Scopus, PubMed, SpringerLink, Wiley Online Library, and Google Scholar. The review shows that kabau seeds and fruit pods contain diverse secondary metabolites, dominated by phenolics, flavonoids, terpenoids, fatty acids (e.g., linoleic and palmitic acids), and sulfur-containing amino acids related to djenkolic acid. These constituents underpin strong in vitro antioxidant activity and support preliminary evidence of antidiabetic, anti-hyperuricemic, and antimicrobial effects in enzyme-based assays and animal models. Ethnobotanical data from Bengkulu and other regions document kabau as a functional food, a remedy for metabolic and digestive disorders, and a component of local cultural identity. Drawing on this evidence, the article proposes a framework for integrating kabau into biology education as a contextual learning resource across topics such as plant morphology, ecology, ethnobotany, phytochemistry, health, and STEM-based inquiry. Integrating local biological resources like kabau is argued to promote scientific literacy, critical thinking, and sustainability awareness while strengthening students’ appreciation of local biodiversity. Further field, experimental, and design-based research is needed to standardize kabau-derived products and to evaluate their effectiveness and safety, as well as their impact on learning outcomes in science education.
The effect of salung leaf extract (Psychotria viridiflora) on blood glucose levels in mice Dara, Niki Dwi Dia; Ruyani, Aceng; Wardana, Rendy Wikrama; Mayub, Afrizal; Karyadi, Bhakti
Jurnal Penelitian Saintek Vol 30, No 2 (2025)
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/jps.v30i2.87757

Abstract

This research is crucial in the context of the rising global prevalence of diabetes, a metabolic disorder that significantly affects public health. This study aims to determine the effect of salung leaf extract (Psychotria viridiflora) on blood glucose levels in hyperglycemic mice (Mus musculus). The study used 25 mice (Mus musculus) weighing 20-40 grams, divided into 5 treatment groups (P). Each group consisted of 5 mice, including a negative control, positive control, and three experimental groups with the administration of P. viridiflora extract at various doses (0.0112; 0.0225; and 0.0337 g/kgBW). Blood glucose measurements were conducted four times, on days 0, 6, 12, and 18, which included an initial glucose measurement, post-alloxan induction, and subsequent measurements after treatment. Among the three tested doses, the highest dose (0.0337 g/kg BW) proved to be the most effective in reducing blood sugar levels in alloxan-induced M. musculus, with effectiveness comparable to the standard drug Glibenclamide. Thus, the extract of P. viridiflora leaves shows strong potential as an alternative herbal antidiabetic therapy for M. musculus.
Pengaruh Pemberian Ekstrak Daun Salung (Psychotria viridiflora) terhadap Glukosa Darah Mencit (Mus musculus) Niki Dwi Dia Dara; Aceng Ruyani; Rendy Wikrama Wardana; Afrizal Mayub; Bhakti Karyadi
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 8 No. 5 (2025): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/zj7jqp95

Abstract

This study aimed to determine the effect of salung (Psychotria viridiflora) leaf extract on blood glucose levels in hyperglycemic mice (Mus musculus). A total of 25 mice weighing 20–40 grams were divided into five treatment groups: P1 (negative control: feed and distilled water), P2 (alloxan 10 mg/kg BW + glibenclamide 0.1 mg/kg BW), P3 (alloxan 10 mg/kg BW + P. viridiflora leaf extract 0.0112 g/kg BW), P4 (alloxan 10 mg/kg BW + P. viridiflora leaf extract 0.0225 g/kg BW), and P5 (alloxan 10 mg/kg BW + P. viridiflora leaf extract 0.0337 g/kg BW). Blood glucose levels were measured four times—on days 0, 6, 12, and 18—covering baseline measurement, post-alloxan induction, and after treatment administration. The results showed that the highest dose (0.0337 g/kg BW) was the most effective in reducing blood glucose levels in alloxan-induced mice, with an effect comparable to the standard drug glibenclamide. In conclusion, P. viridiflora leaf extract has potential as an alternative herbal antidiabetic therapy in M. musculus.  Keywords: Antidiabetic, Blood Glucose, Extract Leaf, Hyperglycemia, Mus musculus, Psychotria viridiflora
Pemberian Jus Buah (Etlingera hemisphaerica) terhadap Pemulihan Eritrosit dan Leukosit Akibat Perlakuan Merkuri Klorida (HgCl2) pada Mencit (Mus musculus) Fatimah Azzahrah; Aceng Ruyani; Deni Parlindungan; Arsela Eko Listiono; Afrizal Mayub
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol. 8 No. 5 (2025): Bioedusains: Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/7ef7hb33

Abstract

This study aims to determine the effect of Etlingera hemisphaerica (honje) fruit juice on the recovery of erythrocyte and leukocyte counts in mice (Mus musculus) exposed to mercuric chloride (HgCl₂). The method used was an experimental design using male mice as test animals, divided into three groups: K1 (control), K2 (honje juice 0.3 mL/20 g BW for 3 days), and K3 (HgCl₂ 5 mg/kg BW + honje juice 0.3 mL/20 g BW for 3 days). The observed parameters were the number of erythrocytes and leukocytes before and after treatment. The results showed that honje juice administration increased the erythrocyte count in group K2, while in group K3, erythrocyte recovery was affected by mercury exposure, making the honje juice less effective, although the values remained within the normal range compared to the control group. In leukocyte count, group K2 showed a stabilizing effect, whereas group K3 experienced an increase due to HgCl₂ exposure. In conclusion, Etlingera hemisphaerica fruit juice has potential as a phytotherapy agent to repair hematological damage caused by heavy metal toxicity, although its effectiveness is influenced by the metabolic condition of the mice and the high concentration of mercury. Keywords: Etlingera hemisphaerica, Erythrocytes, Leukocytes, Mice, Mercuric Chloride
Co-Authors Abas Abas, Abas Abdul Rahman Abdul Rahman Singkam Adityah Wiradinata Afrizal Mayub Ananda, Resti Annisa Puji Astuti Ariefa Primair Yani Ariefa Primair Yani Arsela Eko Listiono ASTUTI, ANNISA PUJI Athika Ulfah Putri Bambang Prayogo, Bambang Bella, Anisa BHAKTI KARYADI Bhakti Karyadi Bhakti Karyadi BHAKTI KARYADI Budi Maryani Budiyanto Budiyanto Budiyanto Budiyanto Cally Piramida, Cally CHOIRUL MUSLIM CHOIRUL MUSLIM Choirul Muslim Cut Kandy Safiera Dara, Niki Dwi Dia Defianti, Aprina Deka Agustina Delviastri Widyana Deni Parlindungan Deni Parlindungan Deshinta Arrova Dewi Desy Hanisa Putri Johan Dewi Jumiarni Dewi, Novi Ratna Diah Suprihatin Dwi Mentari Dwita, Uci Rahma Elsi Adelia Fitri Elvida Sari Yunilarosi Elza Heryensi Endang Widi Winarni Enny Nugraheni Eria Irmawati Erik Perdana Putra Erna Yunita Sari Euis Nursa&#039;adah Fahmizal Fahmizal Fahmizal, Fahmizal Fannyda, Raidatul Fatimah Azzahrah Febrian, Intan Fema Anggriani Fitri April Yanti Fitri April Yanti, Fitri April Ginting, Nesty Apulina Br Hardiansyah, Akbari Harlita Hartuti, Endah Dwi Haryono, M. Henny Johan Henny Johan Heriwati Heriwati Herlina Herlina HERNANI - HERRY HARYANTO Hery Haryanto Hery Suhartoyo Hery Suhartoyo Hery Suhartoyo Heryensi, Elza Hilda Taurina Husein, Ahmad Saddam ICE PURNAMAWATI Ilahi, Fitratul Indah Maryani Irwan Koto, Irwan Irwandi Ansori Irwandi Ansori Iskandar, Aiza Sabina Putri Isytigfarini, Anisah Putri Iwan Setiawan Jabidi Jabidi Julianti, Apri Julita Pasaribu Kartika Sari Kasrina Kasrina Khairunnisa Kurniani, Riska Lazfihma Lazfihma Leditiya Lestari Lia Anggraini Lien A. Sutasurya Lily Ismaini Liya Agustin Umar M. Lutfi Firdaus M. Sutarno Marisadonna Asteria Marliansa, Meiredo Maryani, Budi Mayshah Purnamasari Mayub, Afrizal Melda Nuari Handini MUSNI MARNIS NADYA NADYA Naintyn Novitasari, Naintyn Niki Dwi Dia Dara Nurcahyani Nurhamidah Nurleli Nurleli Nurma Yunita Indriyanti Nursa'adah, Euis Nursaadah, Euis Nurul Astuty Yensi Nur’azizah Prihatiningsih Palindungan, Deni Parlindungan, Deni Primair Yani, Ariefa Purnama, Intan Putri Ramadan Lazuardi Putri, Desy Hanisa Putri, Zakyya Septiana R. R. Sri Astuti Rahyuni Rahyuni Rani, Silvia Reka Susanti Rendy Wikrama Wardana Rendy Wirakrama Reza Wahyuni Rica Yuniar Tanjung Rika Partika Sari Roni Sastiawan Safniyeti Safniyeti Santi Nurul Kamilah Saputra, Fajar Eko Sari, Anjeli Rama Sari, Fenty Fitrian Sari, Gina Aprella Sari, Intan Indah Sari, Nurma Yunita Selpiya, Mega Shabrina, Annida Nurul Sherlo Maulana Sipriyadi Siti Habiburrahmah Ash Shadar Siti Haryani Muayadah Siti Nurdianti Muhajir Sonia, Gina Sri Sudarwati Sukarso, A. A. Sukarso, A.A. Sumiyati - Sumpono Sumpono Sutarno Sutarno Sutarno Sutarno Sutarno, M. Sylvia Rianissa Putri Tendri Ayu Putri Tiyya Susanti Jumaitul Waini Tomi Hidayat, Tomi Trisna, Dwi Martha Uliyandari, Mellyta Untung Priadi Urip Santoso Wardana, Rendy Wikrama Widia Gusti Wiryono Wiryono Wiryono, Wiryono Wulandari, Cessi Yennita Yulinda Fitriani Yunita Arianti Zamzaili Zamzaili Zamzaili Zamzaili